Curriculum Vitae: Dr. Christian Schiineich
`
`Takeru Higuchi Distinguished Professor and Chair,
`Department ofPharmaceutical Chemistry,
`The University ofKansas
`
`Title:
`Date of birth:
`Place of birth:
`Nationality:
`Visa status:
`
`Address:
`
`Ph.D.
`26.03.1962
`Berlin. FRG
`Double Citizenship: German. US.
`US. Citizen
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`
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`stltute)
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`785 864-4880
`email: schoneic@ku.edu
`
`Marital status:
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`Married. 3 children.
`
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`School:
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`1968-1974:
`1974-1980:
`1980:
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`Universig:
`1981-1982:
`1982-1987:
`October 1987:
`January 1990:
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`Schweizerhof—Grundschule Zehlendorf, Berlin
`Amdt—Gynmasium Dahlem, Berlin
`Abitur
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`Material Sciences. Technical University Berlin
`Chemistry. Free University Berlin
`Diplom at the Free University Berlin
`Ph.D. in chemistry with "honors", Technical University Berlin
`
`MW
`"Quantitative kinetic investigations of the reversible H-atom transfer
`between radicals from thiols and biological relevant compounds".
`Research Director: Professor K.-D. Asmus
`
`Professional emploment:
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`11/1987 - 05/1991:
`05/1988 - 08/1988:
`05/1991 - 07/1992:
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`08/ 1992-07/1998:
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`08/1998-07/2003
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`08/2003 -present
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`08/2003-present
`01/2004-07/2004
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`01/2005-present
`01/2010-present
`08/201 l-preseut
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`Research Assistant at the Hahn-Meitner—Institute Berlin. Germany.
`Brunel University Uxbridge. London. England; Dept. of Biochemistry.
`Postdoctoral Fellow (DFG-fellowship): Department of Pharmaceutical
`Chemistry, University of Kansas. Lawrence. KS 66045. USA.
`Assistant Professor. Department of Pharmaceutical Chemistry.
`University of Kansas. Lawrence. KS 66047. USA.
`Associate Professor. Department of Pharmaceutical Chemistry.
`University of Kansas. Lawrence. KS 66047. USA.
`Courtesy Professor. Department of Chemistry
`University of Kansas. Lawrence. KS 66045. USA.
`Professor. Department of Pharmaceutical Chemistry
`Visiting Professor. Department of Inorganic Chemistry. ETH Ziirich.
`Switzerland
`
`Chair. Department of Pharmaceutical Chemistry
`Member. Board of Trustees. KU Center for Research
`Taker" Higuc/Ii Distinguished Professorfor Bioana/vtica/ Chemistry. Department of
`Pharmaceutical Chemistry
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`l
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`MAIA Exhibit 1004
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`MAIA V. BRACCO
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`IPR PETITION
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`"Young Investigator Travel Award" of the Baxendale Fund for attendance of
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` Miller-Conference in England, 1989.
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`"Young Investigator Award" of the Society For Free Radical Research (SFRR)
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`(awarded in Pasadena, CA, USA).
`
`Schering-Award (for the dissertation).
`
`Tiburtius-Award (for the dissertation).
`
`Postdoctoral fellowship of the Deutsche Forschungsgemeinschaft (DFG).
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`Postdoctoral Fellowship from Hoffmann-LaRoche, Nutley, NJ, USA.
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`"Young Investigator Award" of the Society For Free Radical Research (SFRR)
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`(awarded in Sydney, Australia).
`
`"Young Investigator Travel Award" of the Baxendale Fund for attendance of
` Miller-Conference in Italy, 1995.
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`Eli Lilly New Investigator Award in Pharmaceutics 1996 and 1997
`Pfizer Research Scholar Award
`Pfizer Research Scholar Award
`Pfizer Research Scholar Award
`Center for Teaching Excellence Award for Graduate Teaching
`Teacher of the Year, American Association of Colleges of Pharmacy
`Pfizer Research Scholar Award
`Elected Fellow of the American Association of Pharmaceutical Scientists (AAPS)
`Dolph Simons Award in Biomedical Sciences
`Named Takeru Higuchi Distinguished Professor for Bioanalytical Chemistry
`Assessor, Australian Research Council
`Senior Administrative Fellow, The University of Kansas
`Elected Vice-Chair of Gordon Conference on Oxygen Radicals 2020
`Elected Chair of Gordon Conference on Oxygen Radicals 2022
`
`Awards and Honors
`
`
`1989
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`1990
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`1991
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`1991
`1991-1992
`1991-1993
`1994
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`1995
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`1996, 1997
`2001
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`2002
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`2003
`2003
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`2003
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`2004
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`2005
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`2010
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`2011
`2013-present
`2014-2015
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`2018
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`2018
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`Editorial Positions
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`2002-present
`2008-present
`2009-present
`1998-2018
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`2000-2004
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`Past Editorial Positions
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`Editorial Advisory Board, Chemical Research in Toxicology
`
`Advisory Board
`2014-present
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`Professional Organizations
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`Society for Redox Biology and Medicine (SFRBM)
`American Chemical Society (ACS)
`Association of American Pharmaceutical Scientists (AAPS)
`American Society for Photobiology (ASP)
`
`Service
`
`2003-2006
`2005
`
`
`Recent University-wide service:
`
`Editorial Advisory Board J. Pharm. Sci.
`International Editorial Board, Free Radical Biology & Medicine
`Review Editor, Free Radical Research
`Scientific Advisory Board, Experimental Gerontology
`Associate Editor, AAPS PharmSci, now: The AAPS Journal
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`Scientific Advisory Board, Coriolis Pharma, Munich, Germany
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`American Heart Association, Heartland Affiliate, Research Committee
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` Member, Miller Trust Committee (Miller Conference on Radiation Chemistry)
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` Member, Campus Safety Task Force
`2013-2014
` Member, Work group on Driving Discovery and Innovation (DDI) of the
`2010-2012
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`University Strategic Planning Committee
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`
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` Member of the Stakeholder Group for the development of a university-wide faculty activity reporting
`2010-2012
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`system
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`
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`2009-present Member, Board of Trustees KU Center for Research
`2009-2010
` Member, Chancellor's Task Force on Faculty Research Engagement
`2008-present: Member, Internal Advisory Board of the Higuchi Biosciences Center
`2005-2006:
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`Chair, University Committee on Establishment of a Postdoctoral Association at KU
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`Financial Support: Christian Schöneich
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`Active support
`
`a) NIH/FDA (1U01FD005285), Development of an Integrated Mathematical Model for Comparative
`Characterization of Complex Molecule
`d) $ 1,000,000
`
`
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`b) 8.3%, PI, Multi-PI Grant
`
`
`c) 09/08/14-09/07/2017
`e) This grant supports the development of an algorithm which correlates experimental data on chemical and
`physical stability of biological macromolecules with biological activity
`f) No overlap
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`a) Genentech, Mechanisms of protein oxidation
`d) $ 1,074,500
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`b) 2%, PI
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`c) 05/01/10-06/30/2019
`e) This grant supports mechanistic research on thiyl radical-dependent protein oxidation
`f) No overlap
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`a) Genentech, Mechanisms of polysorbate oxidation
`d) $ 400,000
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`b) 2%, PI
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`c) 05/01/14-7/31/2018
`e) This grant supports mechanistic research on radical-dependent polysorbate oxidation
`f) No overlap
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`a) Genentech, Mechanisms of poloxamer oxidation
`d) $ 40,000
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`b) 2%, PI
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`c) 07/01/18-6/301/2019
`e) This grant supports mechanistic research on radical-dependent polysorbate oxidation
`f) No overlap
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`Completed projects in the last five years
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`a) NIH (N01HV00239), "Modification of cardiovascular proteins by metabolic disease"
`b) National Proteomics Center; 5%, PI of subcontract
`d) $ 291,000 for subcontract
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`c) 08/01/10-07/31/2015
`e) This grant supports the investigation of the role of reactive oxygen species in aging in vivo and in vitro.
`f) No overlap
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`a) NIH (3PO1AG12993), Program Project, "Role of Reactive Oxygen Species in Aging"
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`b) 16%, Project Leader of subproject #1 & Co-Director of the Core Facility (PI: E. Michaelis)
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`c) 04/01/1995-03/31/2014
`d) ca. $ 2.5 million for subproject #1
`e) This grant supports the investigation of the role of reactive oxygen species in aging in vivo and in vitro.
`f) No overlap
`
`a) NSF (CHE-0455575), New biologically relevant sulfur radical cation chemistry
`b) 8.33%, PI of subcontract
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`c) 08/01/10-07/31/14
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`d) $ 240,000 direct for subcontract
`e) This grant supported mechanistic studies on sulfur oxidation relevant to beta-amyloid and Alzheimer's Disease
`f) No overlap
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`a) Janssen, Understanding mechanisms of protein oxidation and its effect on physical behavior
`b) 2%, PI
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`c) 02/01/10-01/31/2015
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`d) $ 100,000
`e) This grant supports research on the degradation mechanisms of concentrated and diluted antibody formulations
`f) No overlap
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`a) FDA, Physicochemical and biological evaluations of different IgG1 Fc glycoforms as a model for
`biosimilar comparability analysis
`d) $ 105,000
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`c) 03/01/14-02/28/2015
`b) 2%, Co-PI
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`e) This grant supports the characterization of the effect of glycosylation on the physical and chemical stability of
`proteins
`f) No overlap
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`a) Amgen Inc., Light-induced degradation of proteins: role of thiyl radicals
`b) 2%, PI
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`c) 12/16/06-12/17/2012
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`d) $ 305,000
`e) This grant supports mechanistic research on thiyl radical-dependent protein oxidation
`f) No overlap
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`a) Bristol Myers Squibb, Peptide and protein disulfide bonds as targets for oxidative damage
`b) PI; Graduate Student Fellowship
`c) 05/01/10-04/30/13
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`
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`d) $ 120,000
`f) This grant focuses on mechanism of oxidative damage of disulfide bonds
`e) No overlap
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`in peptides and proteins”, Department of
`
`Invited lectures (since 1992): Christian Schöneich
`
`1. “Iron-thiolate induced oxidation of methionine to methionine sulfoxide in small and medium-sized model
`peptides. Intramolecular catalysis by histidine”, AAPS Meeting, San Antonio, USA, November 1992.
`2. “Methionine oxidation and methionine-catalyzed oxidation in peptides and proteins”, Upjohn Company,
`Kalamazoo, MI, USA, October 18, 1993.
`3. “Methionine oxidation and methionine-catalyzed oxidation
`Biochemistry, University of Kansas, November 1993.
`4. “Neighboring effects in the oxidative modification of peptides and proteins”, Department of Pharmaceutical
`Chemistry, University of Kentucky, Lexington, KY, USA, March 1, 1994.
`5. “Oxidation of methionine in model peptides: catalytic effect of the N-terminal amino group”, Higuchi Meeting,
`Lake of the Ozarks, MO, USA, March 13-16, 1994.
`6. “Free Radicals and Calcium Regulation”, 2nd SEP Symposium on Molecular Mechanisms of Aging, Kansas City,
`MO, USA, May 6-8, 1994.
`7. “Metal-catalyzed oxidation of peptides and proteins: neighboring group effects and catalysis by methionine”, 1st
`Protein Stability Conference, Breckenridge, CO, USA, July 16-21, 1994.
`8. “Methionine oxidation and methionine-catalyzed oxidation in peptides and proteins”, Amgen, Inc., Thousand
`Oaks, CA, USA, October 20, 1994.
`9. “Calmodulin oxidation by ROS alters protein dynamics and function”, 7th Biennial Scientific Meeting, Society
`For Free Radical Research, Sydney, Australia, November 6-10, 1994.
`10. “Can we use sequence and secondary structure to predict the oxidative degradation of proteins”, AAPS, KCDG,
`Kansas City, MO, USA, January 12, 1995.
`11. “Mechanisms of protein oxidation: relation to aging”, University of Kansas, Medical Center, Kansas City, MO,
`USA, February 14, 1995.
`12. Mechanisms of protein oxidation: relation to aging”, University of Düsseldorf, Germany, March 16, 1995.
`13. “Mechanisms of protein oxidation: relation to biological aging”, University of Texas, Medical Branch
`(UTMB), Galveston, TX, March 30, 1995.
`14. “Mechanisms of oxidation and strategies for stabilization of peptide drugs”, Pfizer Central Research, Groton,
`CT, May 19, 1995.
`15. “Mechanisms of protein oxidation: relation to aging”, The Procter & Gamble Company, Miami Valley
`Laboratories, Cincinnati, OH, June 13, 1996.
`16. “Oxidative stress, protein oxidation, and calcium regulation. Implications for apoptosis and biological aging”,
`Istituto Dermopatico Dell’Immacolata, Rome, Italy, July 4, 1995.
`17. Effect of sequence and structure on radical reactions with peptides and proteins: radiation chemistry vs. metal-
`catalyzed oxidation”, 10th International Congress on Radiation Research, Würzburg, Germany, August 27-
`September 1, 1995.
`18. “Mechanisms of free radical reactions with peptides and proteins”, 19th Miller Conference on Radiation
`Chemistry, Cervia/Milano Maritima, Italy, September 16-21, 1995.
`19. “Redox reactions of methionine in peptides and proteins: mechanisms and implications for biological aging”,
`University of Kansas, Analytical Colloquium, Department of Chemistry, October 23, 1995.
`20. “Redox reactions of methionine in peptides and proteins: mechanisms and implications for biological aging”,
`University of Kentucky, Department of Chemistry, Lexington, KY, USA, January 26, 1996.
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`21. “Redox reactions of methionine in peptides and proteins: mechanisms and implications for biological aging”,
`University of Notre Dame, Department of Chemistry and Biochemistry, and Radiation Laboratory, Notre
`Dame, IN, USA, February 1, 1996.
`22. “Mechanisms of metal-catalyzed oxidation of proteins”, Genentech, Inc., South San Francisco, CA, USA,
`March 11, 1996.
`23. “Diastereoselectivity and neighboring group effects in the oxidation of methionine-containing peptides”, 211th
`ACS National Meeting, New Orleans, LA, USA, March 24-28, 1996.
`24. “Mechanisms of free radical reactions with peptides and proteins”, 44th Annual Meeting of the Radiation
`Research Society, Chicago, IL, USA, April 14-17, 1996.
`25. “S-Nitrosothiols”, Kansas IdeA Worksession on “Nitric Oxide in the Nervous System”, Kansas City, MO, May
`24-26, 1996.
`26. “Diastereoselectivity in the free radical oxidation of methionine-containing peptides as a function of peptide
`sequence and sulfur-sulfur interactions”, 7th International Symposium on Organic Free Radicals, Lago di Garda,
`Italy, June 16-21, 1996.
`27. “Reactive oxygen species and biological aging: a mechanistic approach”, Institute for Biomedical Aging
`Research, Austrian Academy of Sciences, Innsbruck, Austria, July 25, 1996.
`28. “About the reaction of sulfur radical cations and superoxide: pH- and isotope effects, and biological relevance”,
`German Radiation Chemistry Meeting, Kloster Nimbschen, Germany, September 22-25, 1996.
`29. “Age-related modifications of skeletal muscle SR Ca-ATPase: the role of nitric oxide and peroxynitrite”, 1st
`International Conference on the Chemistry and Biology of Peroxynitrite, Ascona, Switzerland, May 11-16,
`1997.
`30. “Metal-catalyzed oxidation of human growth hormone”, Eli Lilly & Company, Indianapolis, IN, USA, June 2,
`1997.
`31. “Radical reactions of peptides and proteins and their significance for biological aging”, Max-Planck Group For
`Time-Resolved Spectroscopy, University of Leipzig, Germany, September 12, 1997.
`32. “Mechanisms of free radical reactions of histidine, tyrosine, and methionine in peptides and proteins:
`implications for biological aging”, International Conference on Pulse Investigations in Chemistry, Biology, and
`Physics, Szczyrk, Poland, September 13-19, 1997.
`33. “Metal-catalyzed oxidation of protein pharmaceuticals: mechanisms and preventive strategies”, Faculty of
`Pharmaceutical Sciences, Kyoto University, Kyoto, Japan, November 28, 1997.
`34. “Age-related modification of skeletal muscle SR Ca-ATPase isoforms. The role of nitric oxide and
`peroxynitrite, and physiological consequences”, 2nd International NILS Workshop on Longevity Sciences:
`Roles of proteins in aging and age associated disorders, Obu-shi, Aichi, Japan, November 29-30, 1997.
`35. "Age-related modifications of calmodulin and the skeletal muscle SR Ca-ATPase through reactive oxygen
`species: Mechanisms and functional consequences", Department of Physiology, The University of Texas Health
`Sciences Center, San Antinio, TX, USA, March 2, 1998.
`36. "On the reaction of superoxide with sulfide radical cations. Mechanisms and biological relevance". 215th ACS
`National Meeting (Division of Organic Chemistry), Dallas, TX, USA, March 29-April 2, 1998.
`37. "Age-related in vivo nitration and thiol modification of SR Ca-ATPase isoforms: chemical characterization,
`functional consequences, and in vivtro simulation", Symposium on Neurodegeneration, Aging and Oxidative
`Stress, Lawrence, KS, USA, May 26-27, 1998.
`38. "Reactions of sulfide radical cations with superoxide: mechanism, diastereoselectivity, and biological
`relevance". Workshop Highlights on Radical Reactivity; Bologna, Italy, July 3, 1998.
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`39. "Diastereoselective reactions of sulfide radical cation complexes with oxygen and superoxide radical anions",
`EUCHEM Conference on Organic Free Radicals; Grottaferrata, Italy, July 4-9, 1998.
`40. "Mechanisms of protein oxidation by reactive oxygen species. Relevance for the pharmaceutical and biological
`sciences", Department of Pharmaceutical Chemistry, University of Heidelberg, Germany, July 9, 1998.
`41. "Mechanisms of methionine oxidation in peptides and proteins", Scios Inc., Mountain View, CA, July 14, 1998.
`42. "Mechanisms of reactions of sulfide radical cation complexes with superoxide and oxygen", Workshop on
`Reactive Intermediates in Sulfur Chemistry; Poznan, Poland, August 23-26, 1998.
`43. "Reactive oxygen species in aging: mechanistic investigation using radiation and photochemistry", Institute for
`Nuclear Chemistry and Technology, Warsaw, Poland, September 11, 1998.
`44. "Free Radicals in Aging", Department of Chemistry, Analytical Chemistry Seminars, University of Kansas,
`Lawrence, KS, USA, September 21, 1998.
`45. "Therapeutic Proteins: stability, legal aspects, and new applications", Department of Pharmaceutical Chemistry,
`ETH Zürich, Switzerland, September 29, 1998.
`46. "Contributions of radiation chemistry to the solution of current problems in chemistry, biochemistry, and
`biology", Symposium in honor of the 30th anniversary of the Institute for Theoretical Chemistry and 25th
`anniversary of the Institute of Radiation Chemistry at the University of Vienna, Vienna, Austria, November 20,
`1998.
`47. “Mechanisms of protein oxidation as a tool for the development of stable formulations”, 3rd Symposium on the
`Analysis of Well Characterized Biotechnology Pharmaceuticals (WCBP), Washington, D.C., USA, January 5-
`8, 1999.
`48. “Effect of sequence and structure on the oxidation of methionine in peptides and proteins”, National Institutes
`of Health (NIHLB), Bethesda, MD, USA, January 7, 1999.
`49. “Peroxynitrite as a mediator of oxidative stress and biological dysfunction”, Gray Laboratory Cancer Research
`Trust, Mount Vernon Hospital, Northwood, England, January 14, 1999.
`50. “Understanding oxidation mechanisms as a tool for the rational design of oxidation-resistant drugs and drug-
`formulations”, Pfizer, Inc., Central Research Division, Groton, CT, USA, March 24, 1999.
`51. “Peroxynitrite modification of protein thiols: oxidation, nitrosylation, and S-glutathiolation of functionally
`important Cys residue(s) of the SR Ca-ATPase”, 2nd International Conference on the Chemistry and Biology of
`Peroxynitrite, Crete, Greece, May 15-19, 1999.
`52. “Protein oxidation: Mechanisms, analysis, and strategies for inhibition”, Symposium on Covalent Degradation
`of Proteins, Amgen, Inc., Thousand Oaks, CA, June 1, 1999.
`53. “Diastereoselective oxidation of peptides and proteins by reactive oxygen species”, 11th International Congress
`of Radiation Research, Dublin, Ireland, July 18-23, 1999.
`54. “Radiation damage to proteins and amino acids”, 6th International Congress on Amino Acids, Bonn, Germany,
`August 3-7, 1999.
`55. “Calcium homeostasis during biological aging: modulation through redox modification of calcium-regulating
`proteins”, Dept. of Biochemistry, University of Konstanz, Germany, August 5, 1999.
`56. “Mechanisms of protein oxidation as a tool for the development of stable formulations”, Chiron Corporation,
`Emeryville, CA, USA, August, 16, 1999.
`57. “Peroxynitrite modification of protein thiols”, 6th Annual Meeting of the Oxygen Society, New Orleans, LA,
`USA, November 18-22, 1999.
`58. “Mechanisms of methionine oxidation in peptides and proteins”, Searl, Skokie, IL, December 17, 1999.
`59. “The mysterious chemistry of biological aging”, Boston University Medical Center, Vascular Biology Unit,
`Boston, MA, February 29, 2000.
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`60. “Mechanisms and inhibition of protein oxidation”, Infimed Therapeutics, Cambridge, MA, March 1, 2000.
`61. “Reactive oxygen species and biological aging: Challenge and opportunity for radiation chemical research”,
`University of Vienna, Austria, March 20, 2000.
`62. “The mysterious chemistry of biological aging”, Institute for Biomedical Aging Research, Innsbruck, Austria,
`March 21, 2000.
`63. “New mechanisms of oxidation of methionine in peptides and proteins”, Biannual Meeting of German
`Radiation Chemists, Bad Honnef, Germany, March 22-25, 2000.
`64. "Biological aging and age-related diseases: challenges and opportunities for radiation chemistry-based free
`radical research", Gordon Research Conference " Radiation Chemistry", Plymouth , NH, June 24-30, 2000.
`65. "Selectivities of the reaction of NO and ONOO- with SERCA. Implications for aging and cardiovascular
`disease", Symposium on Reactive Oxygen and Nitrogen Species in Chemistry and Biology, ETH Zürich,
`Switzerland, July 13, 2000.
`66. "The role of thiyl and thioether radicals in DNA and protein damage", Consiglio Nazionale delle Ricerche,
`Bologna, Italy, July 14, 2000.
`67. "The mysterious chemistry of biological aging", University of Bayreuth, Germany, July 21, 2000.
`68. "Protein oxidation on the timescale of nanoseconds, seconds, and years", Bodega Marine Biology Institute (UC
`Davis), Bodega Bay, CA, November 15, 2000.
`69. "When radicals react with proteins: theory, aging, and pathology", University of Kansas Medical Center,
`Kansas City, MO, January 26, 2001.
`70. "When radicals react with proteins: theory, aging, and pathology", Boston University Medical Center, Whitaker
`Research Institute, Boston, MA, January 31, 2001.
`71. "Modification of SERCA2 Ca-ATPases by reactive nitrogen species", Gordon Research Conference
`"Biochemistry of Nitric Oxide", Ventura, CA, February 4-9, 2001.
`72. "Protein oxidation in solution, polymers, and the solid state", Pharmaceutical Conference of the Americas,
`Orlando, Fl, March 25-28, 2001.
`73. "Thiyl radicals as intermediates and chain carriers in the oxidation of proteins and DNA", Department of
`Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL, May 10, 2001.
`74. "Structural properties control Tyr nitration and Cys modification of SERCA by reactive nitrogen species", 3rd
`International Conference on Peroxynitrite and Reactive Nitrogen Species in Biology and Medicine, Asilomar
`Conference Grounds, Pacific Grove, CA, May 27-31, 2001.
`75. "Reactive oxygen species, NO, and protein oxidation during biological aging", Workshop on Processes of
`Aging in Plants", Bayreuth, Germany, June 22-23, 2001.
`76. "Effect of sequence and structure on the oxidation of cysteine, histidine, and methionine in proteins", Bayer
`Corporation, Berkeley, CA, November 16, 2001.
`77. "Molecular parameters controlling the oxidative degradation of pharmaceuticals", Bristol Myers Squibb, New
`Brunswick, NJ, February 5, 2002.
`78. "Oxidative inactivation of Ca-ATPase and characterization of sulfur oxidation sites in proteins", Gordon
`Conference, Oxygen Radicals, Ventura, CA, February 10-15, 2002.
`79. “Sulfur-centered free radicals in protein and nucleotide oxidation: relevance to biological aging and
`Alzheimer’s disease”, Department of Chemistry, University of Calgary, Canada, March 18, 2002.
`80. “Methionine radical cations and sulfuranyl radicals in -amyloid peptide toxicity and Alzheimer’s disease”, 18th
`International Radical Ion Conference, Heigenbrücken, Germany, June 16-21, 2002.
`81. "Molecular parameters controlling the oxidative degradation of pharmaceuticals", Novartis, East Hanover, NJ,
`September 13, 2002.
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`82. “Characterization of post-translational modifications in large membrane proteins and protein aggregates of low
`solubility: 3-nitrotyrosine on glycogen phosphorylase B and SERCA”, 2nd North American Bioanalytical
`Forum, Kansas City, MO, September 29-October 2, 2002.
`83. “Characterization of post-translational modifications in large membrane proteins and protein aggregates of low
`solubility: 3-nitrotyrosine on glycogen phosphorylase B and SERCA”, Midwest Regional ACS Meeting,
`Lawrence, KS, October 23-25, 2002.
`84. “Molecular parameters controlling the oxidative degradation of pharmaceuticals”, Merck, West Point, PA,
`December 2, 2002.
`85. Methionine radical cations and sulfuranyl radicals in -amyloid peptide toxicity and Alzheimer’s disease”,
`Stokes Research Institute, Children’s Hospital of Philadelphia, University of Pennsylvania, Philadelphia, PA,
`December 3, 2002.
`86. “Selective post-translational modification of proteins during oxidative stress: proteomics and reaction
`mechanisms”, Proteomics Workshop, University of Kansas, Medical Center, January 29, 2003.
`87. “Sequence and conformation control selective free radical reactions in peptides and proteins: a free radical
`mechanism of Alzheimer's disease beta-amyloid toxicity?”. Department of Chemistry, University of Rochester,
`NY, March 7, 2003.
`88. “Benefits of a mechanistic and structures approach to protein instability”, 2nd Annual Conference of the
`Institute for International Research (IIR) “Protein and Peptide Formulation Strategies”, Boston, MA March 31-
`April 2, 2003.
`89. “Why develop an ICAT reagent for the identification and quantification of 3-nitrotyrosine in vivo?”, Boston
`University Medical Center, April 2, 2003.
`90. “Reactive oxygen species and selective protein oxidation in biological aging and disease: lessons from
`proteomic studies and physical-organic chemistry”, Gill Lecture 2003, Department of Chemistry, Indiana
`University, Bloomington, IN, April 9, 2003.
`91. “Conformational control of sulfur radical formation and reactivity in peptides and proteins: implications for
`protein stability, NO biochemistry, and Alzheimer's disease”, Plenary Lecture at European Young Investigator
`Conference at Słubice, Poland, May 7-11, 2003.
`92. “Age-dependent selective protein modification and repair”, Workshop on proteomic approaches to oxidative
`stress and biological aging, Pacific Northwest National Laboratories, Richland, WA, May 27-28, 2003.
`93. “Conformational dynamics of Alzheimer’s b-amyloid peptide support free radical formation and neurotoxicity:
`mechanism and proof of concept”, Protein Stability Conference, Breckenridge, CO, July 17-19, 2003.
`94. “Oxidative and photolytic reactions of peptides and proteins in solution and the solid state”, IBC 3rd
`International Conference Formulation Strategies for Biopharmaceuticals, Philadelphia, PA, September 22-24,
`2003.
`95. “Proteomics of posttranslational protein modifications in aging and disease”, Higuchi Biosciences Center
`Science Talks, Lawrence, KS, November 18, 2003.
`96. “Free radical generation by the Alzheimer’s disease -amyloid peptide: novel mechanisms of methionine
`oxidation”, Plenary Lecture at the 10th Annual Meeting of the Society For Free Radical Biology and Medicine,
`Seattle, WA, November 20-24, 2003.
`97. “Free radical generation by the Alzheimer’s disease -amyloid peptide: a reaction between organic sulfide and
`Cu(II)?”, Federal Institute of Technology (ETH) Zürich, Switzerland, April 6, 2004.
`98. “Biological targets of peroxynitritre in vivo and physiological implications”, Federal Institute of Technology
`(ETH) Zürich, Switzerland, May 4, 2004.
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`10
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`99. “Oxidative and photolytic degradation of peptides and proteins in solution, polymers and the solid state”,
`GPEN Meeting, Kyoto, Japan, May 26-28, 2004.
`100. “Are peptide and protein radicals involved in pathologic processes?”, ISOFR 9 (International Symposium on
`Organic Free Radicals), Porto Vecchio, Korsika (France), June 6-11, 2004.
`101. “Molecular parameters controlling oxidative degradation of pharmaceuticals in solution and the solid state”,
`Boehringer Ingelheim, Ridgefield, CT, September 8, 2004.
`102. “Oxidative protein modifications in cerebellar aging”, 3rd North American Bioanalytical Forum (NABF),
`Kansas City, MO, September 12-15, 2004.
`103. “Oxidative and photolytic degradation of peptides and proteins in solution and the solid state”, IBC’s 4th
`Annual Formulation Strategies for Protein Therapeutics, Boston, MA, October 4-6, 2004.
`104. “Proteomic analysis of age-dependent protein nitration in vivo”, Oxidative Post-Translational Modifications in
`the Cardiovascular System, Boston, MA, October 6-8, 2004.
`105. “Proteomic characterization of protein targets for reactive nitrogen species in aging tissue”, Nathan Shock
`Center Conferences on Aging, Bandera, TX, October 14-17, 2004.
`106. “Oxidative and photolytic degradation of peptides and proteins”, Nektar Therapeutics, San Carlos, CA, October
`25, 2004.
`107. “Proteomic and mechanistic analysis of protein oxidation in biological aging”, National Institutes of Health,
`NHLBI, Bethesda, MD, November 1, 2004.
`108. “Proteomic and mechanistic analysis of protein oxidation in biological aging”, Pacific Northwest National
`Laboratories, Richland, WA, November 16, 2004.
`109. “Oxidative and photolytic degradation of proteins in solution and the solid state”, Eli Lilly, Indianapolis, IN,
`December 3, 2004.
`110. “Protein targets for NO-dependent modification in vivo: a proteomic and mechanistic analysis”, NitroMed,
`Lexington, MA, December 6, 2004.
`111. “Proteomic and mechanistic analysis of protein oxidation in biological aging”, Oklahoma Medical Research
`Foundation, Oklahoma City, OK, December 9, 2004.
`112. “Reactive oxygen species and free radicals: from physiologic necessity to pathologic burden”, Strathclyde
`University, Glasgow, Scotland, March 14, 2005.
`113. “Reactive oxygen species and free radicals: from physiologic necessity to pathologic burden”, Oklahoma
`University Health Sciences Center, Medicine Endocrinology, Oklahoma City, OK, April 21, 2005.
`114. “Oxidative and photolytic degradation of peptides and proteins”, Pfizer Global Biologics, St. Louis, MO, June
`15, 2005.
`115. “Reactive oxygen species and free radicals: from physiologic necessity to pathologic burden”, University of
`Calgary, Calgary, Canada, June 30, 2005.
`116. “Thiyl and sulfide radical reactions in the selective oxidative modification of peptides and proteins: signaling,
`regulation and biological damage”, Gordon Research Conference on Free Radical Reactions, Holderness
`School, Plymouth, NH, July 3-8, 2005.
`117. “Redox modification of protein tyrosine and cysteine residues: proteomic and mechanistic studies",
`Cardiovascular Proteomics Center, Boston University Medical School, Boston, MA, July 8, 2005.
`118. “Oxidative modification of proteins in vitro and in vivo: analytical and mechanistic challenges”, 16th
`International Symposium on Pharmaceutical and Bioanalytical Analysis (PBA), Baltimore, Maryland, August
`28-31, 2005.
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`11
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`119. “Peptide and protein radicals in biologic pathologic processes: reaction mechanisms studied by radiation- and
`photochemistry and mass spectrometry”, 24th Miller Conference on Radiation Chemistry, La Londe les Maures,
`France, September 10-15, 2005.
`120. “Mechanistic characterization of protein oxidation hot spots”, Department of Pharmaceutical Sciences,
`University of Colorado Health Sciences Center, Denver, Colorado, January 25, 2006.
`121. “Protein modification by reactive oxygen and reactive nitrogen species in vivo and in vitro”, SULFRAD
`Meeting, Paris, France, February 1-5, 2006.
`122. “Defining molecular pathways for protein dysfunction in aging and disease using global and targeted
`proteomics”, University of Arizona, School of Pharmacy, Department of Medicinal Chemistry, Tucson, AZ,
`March 9, 2006.
`123. “Oxidation mechanisms of methionine important for -amyloid toxicity, Alzheimer’s disease, and the
`characterization of protein oxidation hot spots”, 20th International Symposium on Radical Ion Reactivity, Villa
`Mondragone (Monteprozio Catone), Rome, Italy, July 2-6, 2006.
`124. “Protein oxidation hot spots controlled by structural parameters”, ACS ProSpectives Conference Successful
`protein therapeutics: the interconnection of formulation, development and manufacturing, San Diego, CA, July
`23-26, 2006.
`125. “Selective protein degradation through light, peroxides, and transition metals: reaction mechanisms and
`structure-reactivity relationships”, Amgen, Inc., Thousand Oaks, CA, September 19, 2006.
`126. “Proteomic analysis of age-dependent protein tyrosine nitration: a novel ICAT method selective for 3-
`nitrotyrosine”, 2nd Meeting on Oxidative Post-translational Modifications of Proteins in Cardiovascular
`Disease, Boston, MA, October 3-6, 2006.
`127. “Oxidative protein modifications in aging: proteomic and functional studies, and development of new analytical
`methodology”, CS&B Seminar Series, University of Texas Health Sciences Center, San Antonio, TX, October
`31, 2006.
`128. “Post-translational modificatio