November 2015
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`RICHARD BRUCE VAN BREEMEN
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`Professor of Medicinal Chemistry
`Department of Medicinal Chemistry and Pharmacognosy
`College of Pharmacy
`University of Illinois at Chicago
`833 South Wood Street
`Chicago, Illinois 60612-7231
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`DOB: 11-30-57
`Telephone: (312) 996-9353
`FAX:
`(312) 996-7107
`email: breemen@uic.edu
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`EDUCATION
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`1980-1985
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`Ph.D. 1985, in Pharmacology
`Johns Hopkins University School of Medicine
`Baltimore, Maryland
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`1976-1980
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`B.A. 1980, in Chemistry, with honors, German minor
`Oberlin College, Oberlin, Ohio
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`PROFESSIONAL EXPERIENCE
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`2014-present Matthias C. Lu Collegiate Professor of Pharmacy
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`2014-present Visiting Professor, Tongji School of Pharmacy, Huazhong University of Science
`and Technology, Wuhan, China
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`2000-present Professor of Medicinal Chemistry and Pharmacognosy
`College of Pharmacy, University of Illinois at Chicago, Chicago, IL
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`2002-present Academic Director, Mass Spectrometry, Metabolomics and Proteomics Facility,
`Research Resources Center, University of Illinois at Chicago, Chicago, IL
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`2010-present Director, Mass Spectrometry, Metabolomics and Proteomics Facility, University
`of Illinois Cancer Center, IL
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`2011-present Director, UIC/NIH Center for Botanical Dietary Supplements Research,
`University of Illinois at Chicago, Chicago, IL
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`2001-present Assistant to the Director of the Research Resources Center, University of Illinois
`at Chicago, Chicago, IL
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`2010-present Editorial Board, Assay and Drug Development Technologies
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`2010-present Editor-in-Chief emeritus, Combinatorial Chemistry & High Throughput
`Screening
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`2013-present Regional Editor, Biomedical Chromatography
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`2010-2012 Assistant Head for Curricular Affairs, Department of Medicinal Chemistry and
`Pharmacognosy, University of Illinois at Chicago, Chicago, IL
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`2006-2013
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`Editorial Board, Biomedical Chromatography
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`1997-2010
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`Editor-in-Chief, Combinatorial Chemistry & High Throughput Screening
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`1994-2000 Associate Professor of Medicinal Chemistry
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`College of Pharmacy, University of Illinois at Chicago, Chicago, IL
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`1986-1993 Assistant Professor of Chemistry, Member of the Biotechnology Faculty
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`Director of the Mass Spectrometry Laboratory for Biotechnology Research
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`North Carolina State University, Raleigh, NC.
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`1985-1986
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`1980-1985
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`Postdoctoral Fellow. Middle Atlantic Mass Spectrometry Laboratory, NSF
`Regional Instrumentation Facility, Johns Hopkins University School of Medicine,
`Baltimore, MD. (Laboratory of Catherine Fenselau and Robert Cotter)
`Visiting Assistant Professor of Chemistry
`North Carolina State University, Raleigh, NC.
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`Predoctoral research, Dept. of Pharmacology Johns Hopkins University School
`of Medicine. Baltimore, MD. Dissertation: "Electrophilic Reactions of 1-O-Acyl
`Glucuronides." (Advisor, Catherine Fenselau)
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`1979-1980 Honors research, Department of Chemistry, Oberlin College
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`Oberlin, OH. Synthesis of spirocyclic lactones as analogs of insect pheromones
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`1979
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`Research assistant, Department of Pharmacology, Toxicology Center, Univ. of
`Iowa, Iowa City, IA. Quantitation of metabolites of diphenylhydantoin in human
`serum using GC/MS. (Laboratory of L.J. Fischer)
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`HONORS AND AWARDS
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`NIH Predoctoral Fellowship, 1980-1984
`Phi Lambda Upsilon (Chemistry Honor Society)
`Award for Outstanding Paper at the Third North American Meeting of the International Society
`for the Study of Xenobiotics. San Diego, CA, October 21-25,1990
`Named by the Editors of Spectroscopy as one of 19 “Bright Young Stars” in analytical
`spectroscopy. (“Perspectives on 10 Years in Spectroscopy,” Spectroscopy, 10, 52-61, 1995)
`Invited speaker, 11th International Carotenoid Conference, Leiden, The Netherlands, 1996
`Invited speaker, International Convention on Pharmaceutical Sciences Commemorating the 50th
`Anniversary of the Pharmaceutical Society of Korea. Seoul, Korea, 2001
`Outstanding Paper Award, 88th American Oil Chemists Society Annual Meeting & Expo.
`Seattle, WA, May 11-14, 1997
`Linus Pauling Institute Seminar Series Speaker. Oregon State University, Corvallis, OR,
`February 16, 2006
`University Scholar Award. University of Illinois, 2003-2006
`AOAC Presidential Task Force on Dietary Supplements, 2009-2011
`AOAC International Harvey W. Wiley Award, 2008
`AOAC International Outstanding Dietary Supplement Methods Panel Chair, 2010
`Invited speaker, Charles E. Dohme Memorial Symposium, Johns Hopkins University School of
`Medicine. November 20, 2013
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`PUBLICATIONS
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`1. Papers
`1.
`Cotter RJ, van Breemen R, Yergey J, Heller D. Thermal, laser, and fast atom desorption. Int.
`J. Mass Spectrom. Ion Proc. 46, 395-8 (1983).
`van Breemen RB, Snow M, Cotter RJ. Time resolved laser desorption mass spectrometry, I.
`Desorption of preformed ions. Int. J. Mass Spectrom. Ion Phys. 49, 35-50 (1983).
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`Gibson W, van Breemen R, Fields A, LaFemina R, Irmiere A. D,L-α-
`Difluoromethylorinithine inhibits human cytomegalovirus replication. J. Virology. 50, 145-
`54 (1984).
`van Breemen RB, Tabet J-C, Cotter RJ. Characterization of oxygen-linked glucuronides by
`laser desorption mass spectrometry. Biomed. Mass Spectrom. 11, 278-83 (1984).
`van Breemen RB, Fenselau C. Acylation of albumin by 1-O-acyl glucuronides. Drug Metab.
`Dispos. 13, 318-20 (1985).
`van Breemen RB, Fenselau CC, Dulik DM. Activated Phase II metabolites: Comparison of
`alkylation by 1-O-acyl glucuronides and acyl sulfates. in Biological Reactive Intermediates
`III, ed. by JJ. Kocsis, DJ. Jollow, CM. Whitmer, JO Nelson, and R. Snyder. pp. 423-9,
`Plenum Publishing Corp., New York, 1986.
`van Breemen RB, Fenselau C. Reaction of 1-O-acyl glucuronides with 4-p-
`(nitrobenzyl)pyridine. Drug Metab. Dispos. 14, 197-201 (1986).
`van Breemen RB, Fenselau C, Mogilevsky W, Odell GB. Reaction of bilirubin glucuronides
`with serum albumin. J. Chromatogr. 383, 387-92 (1986).
`van Breemen RB, Fenselau C, Cotter RJ, Curtis A.J., Connolly G. Derivatives of
`dicyclopentadiene in ground water. Biomed. Environ. Mass Spectrom. 14, 97-102 (1987).
`Fenselau C, van Breemen R, Bradow G, Stogniew M. Acyl-linked glucuronides as reactive
`metabolites. Fed. Proc. 46, 2436-9 (1987).
`van Breemen RB, Martin LB, Schreiner AF. Comparison of electron impact, desorption
`chemical ionization, field desorption, and fast atom bombardment mass spectra of nine
`monosubstituted Group VI metal carbonyls. Anal. Chem. 60, 1314-8 (1988).
`van Breemen RB, Stogniew M, Fenselau C. Characterization of acyl-linked glucuronides by
`electron impact and fast atom bombardment mass spectrometry. Biomed. Environ. Mass
`Spectrom. 17, 97-103 (1988).
`van Breemen RB. Fast atom bombardment mass spectrometry with B/E linked scanning of
`ether- and thiophenol-linked glucuronides. In Cellular and Molecular Aspects of
`Glucuronidation, ed. by G. Siest, J. Magdalou, B. Burchell, vol. 173, pp. 211-9, Colloque
`INSERM/John Libbey Eurotext Ltd., 1988.
`van Breemen RB, Le JC. Enhanced sensitivity of peptide analysis by fast atom bombardment
`mass spectrometry using nitrocellulose as a substrate. Rapid Commun. Mass Spectrom. 3, 20-
`4 (1989).
`van Breemen RB, Martin LB, Schreiner AF. Formation of Negative ions of monosubstituted
`Group VIB pentacarbonyls during fast atom bombardment mass spectrometry. Org. Mass
`Spectrom. 25, 3-8 (1990).
`16. Goodlett DR, Armstrong FB, Creech RJ, van Breemen RB. Formylated peptides from
`cyanogen bromide digests identified by fast atom bombardment mass spectrometry. Anal.
`Biochem. 186, 116-20 (1990).
`van Breemen RB, Wheeler JJ, Boss WF. Identification of carrot inositol phospholipids by
`fast atom bombardment mass spectrometry. Lipids, 25, 328-334 (1990).
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`Freeman HS, van Breemen RB, Esancy J F, Hao Z, Ukponmwan DO, Hsu WN. Fast atom
`bombardment and desorption chemical ionization mass spectrometry in the analysis of
`involatile textile dyes. Textile Chemist and Colorist, 22, 23-28 (1990).
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`19. Martin LB, Schreiner AF, van Breemen RB. Characterization of cisplatin adducts of
`oligonucleotides by fast atom bombardment mass spectrometry. Anal. Biochem. 193, 6-15
`(1991).
`van Breemen RB, Martin LB, Le JC. "Continuous-flow fast atom bombardment mass
`spectrometry of oligonucleotides." J. Am. Soc. Mass Spectrom. 2, 157-163 (1991).
`van Breemen RB, Canjura FL, Schwartz SJ. High-performance liquid chromatography-
`continuous-flow mass spectrometry of chlorophyll derivatives. J. Chromatogr. 542, 373-383
`(1991).
`van Breemen RB, Bartlett MG, Tsou Y, Culver C, Swaisgood H, Unger SE. Degradation of
`peptide drugs by immobilized digestive proteases. Drug Metab. Dispos. 19, 683-690 (1991).
`van Breemen RB, Canjura FL, Schwartz SJ. Identification of chlorophyll derivatives by mass
`spectrometry. J. Agric. Food Chem. 39, 1452-1456 (1991).
`Freeman HS, Hao Z, Sokolowska-Gajda J, van Breemen RB. Matrix selection in the FAB
`mass spectrometric analysis of synthetic dyes. Dyes and Pigments, 16, 317-327 (1991).
`van Breemen RB, Huang C-H, Bumgardner CL. Continuous-flow fast atom bombardment
`and field desorption mass spectrometry of oligomers of 3,3,3-trifluorophenylpropyne. Anal.
`Chem. 63, 2577-2580 (1991).
`26. Bumgardner CL, Huang C-H, van Breemen RB. Characterization of oligomers of 3,3,3-
`trifluoro-1-phenylpropyne and 1-phenylpropyne by mass spectrometry. J. Fluor. Chem. 56,
`175-187 (1992).
`Schmitz HH, van Breemen RB, Schwartz SJ. Applications of fast atom bombardment mass
`spectrometry (FAB-MS) and continuous-flow FAB-MS to carotenoid analysis. Methods
`Enzymol. 213, 322-336 (1992).
`van Breemen RB, Davis RG. Rates of peptide proteolysis measured using liquid
`chromatography and continuous-flow fast atom bombardment mass spectrometry. Anal.
`Chem. 64, 2233-2237 (1992).
`van Breemen RB, Schmitz HH, Schwartz SJ. Continuous-flow fast atom bombardment liquid
`chromatography/mass spectrometry of carotenoids. Anal. Chem. 65, 965-969 (1993).
`Saljoughian M, Williams PG, Morimoto H, Goodlett DR, van Breemen RB. Tritiated
`diimide: A regio- and stereo-selective tritium labeling reagent. J. Chem. Soc., Chem.
`Commun. 414-416 (1993).
`31. Oommen TV, Petrie EM, van Breemen RB, Haney CA. Analysis of furanic compounds from
`cellulose aging by GC-MS, and attempts to correlate with degree of polymerization. CIGRE
`Paper 110-02, CIGRE Symposium on Diagnostic and Maintenance Techniques. Berlin,
`Germany, April 19-21. 1993.
`32. Blackburn RK, van Breemen RB. Degradation of the cyclic peptide antibiotic lysobactin by
`immobilized digestive proteases. Drug Metab. Dispos. 21, 573-579 (1993).
`van Breemen RB, Tsou Y, Connolly G. Oxidation of dicyclopentadiene in surface water.
`Biol. Mass Spectrom. 22, 579-584 (1993).
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`Spanos GA, Schwartz SJ, van Breemen RB, Huang C-H. High-performance liquid
`chromatography with light-scattering detection and desorption chemical-ionization tandem
`mass spectrometry of milk fat triacylglycerols. Lipids, 30, 85-90 (1995).
`van Breemen RB, Schmitz HH, Schwartz SJ. Fast atom bombardment tandem mass
`spectrometry of carotenoids. J. Agric. Food Chem. 43, 384-389 (1995).
`van Breemen RB, Jiang O, Tidwell RR, Hall JE, Brewer TG. Fast atom bombardment
`tandem mass spectrometry of the anti-parasitic agent pentamidine and its oxygenated
`metabolites. J. Mass Spectrom. 30, 549-556 (1995).
`van Breemen RB. Electrospray liquid chromatography-mass spectrometry of carotenoids.
`Anal. Chem. 67, 2004-2009 (1995).
`38. Thomas VR, Schreiner AF, van Breemen R, Xie TY, Chen CL, Gratzl JS. Photolytic
`dechlorination of 4-chlorophenol using an ArF* excimer laser. Holzforschung, 49, 139-145
`(1995).
`van Breemen RB. Advances in carotenoid analysis: Electrospray liquid chromatography-
`mass spectrometry using C30 reversed phase HPLC. Carotenoid News, 5, 8-9 (1995).
`van Breemen RB, Huang CH, Lu ZZ, Rimando A, Fong HHS, Fitzloff JF. Electrospray
`liquid chromatography/mass spectrometry of ginsenosides. Anal. Chem. 67, 3985-3989
`(1995).
`van Breemen RB. Innovations in carotenoid analysis using liquid chromatography/mass
`spectrometry. Anal. Chem. 68, 299A-304A (1996). *This paper was featured on the cover
`page of this issue of Analytical Chemistry.
`van Breemen RB, Huang C-H. High performance liquid chromatography-electrospray mass
`spectrometry of retinoids. FASEB J. 10, 1098-1101 (1996).
`van Breemen RB, Huang C-H, Tan Y, Sander LC, Schilling AB. Liquid chromatography/mass
`spectrometry of carotenoids using atmospheric pressure chemical ionization. J. Mass
`Spectrom. 31, 975-981 (1996).
`Shen L, Pisha E, Huang Z, Pezzuto JM, Krol E, Alam Z, van Breemen RB, Bolton JL.
`Bioreductive activation of catechol estrogen-ortho-quinones: Aromatization of the B ring in
`4-hydroxyequilenin markedly alters quinoid formation and reactivity. Carcinogenesis, 18,
`1093-1101 (1997).
`van Breemen RB, Huang C-H, Nikolic D, Woodbury CP, Zhao YZ, Venton DL. Pulsed
`ultrafiltration electrospray mass spectrometry: A new method for screening combinatorial
`libraries. Anal. Chem. 69, 2159-2164 (1997).
`46. Zhao YZ, van Breemen RB, Nikolic D, Huang C-R, Woodbury CP, Schilling A, Venton DL.
`Screening solution-phase combinatorial libraries using pulsed ultrafiltration/ electrospray mass
`spectrometry. J. Med. Chem. 40, 4006-4012 (1997).
`Shen L, Qiu S, van Breemen RB, Zhang F, Chen Y, Bolton JL. Reaction of the Premarin
`metabolite 4-hydroxyequilenin semiquinone radical with β’-deoxyguanosine: Formation of
`unusual cyclic adducts. J. Am. Chem. Soc. 119, 11126-11127 (1997).
`van Breemen RB. Liquid chromatography/mass spectrometry of carotenoids. Pure Appl.
`Chem. 69, 2061-2066 (1997).
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`49. Zhang HQ, Dixon RP, Marletta MA, Nikolic D, van Breemen R, Silverman RB. Mechanism
`of inactivation of neuronal nitric oxide synthase by N-allyl-L-arginine. J. Am. Chem. Soc.
`119, 10888-10902 (1997).
`50. Bolton JL, Pisha E, Shen L, Krol E, Iverson S, Huang C-R, van Breemen RB, Pezzuto JM.
`The reactivity of o-quinones which do not isomerize to quinone methides correlates with
`alkylcatechol-induced toxicity in human melanoma cells. Chem.-Biol. Interact. 106, 133-148
`(1997).
`Shen L, Qiu S, Chen Y, Zhang F, van Breemen RB, Nikolic D, Bolton JL. Alkylation of β’-
`deoxynucleosides and DNA by the Premarin metabolite 4-hydroxyequilenin semiquinone
`Radical. Chem. Res. Toxicol. 11, 94-101 (1998)
`van Breemen RB, Nikolic D, Xu X, Xiong Y, van Lieshout M, West CE, Schilling AB.
`Development of a method for quantitation of retinol and retinyl palmitate in human serum
`using high performance liquid chromatography-atmospheric pressure chemical ionization
`mass spectrometry. J. Chromatogr. A, 794, 245-251 (1998).
`van Breemen RB, Nikolic D, Bolton JL. Metabolic screening using on-line ultrafiltration
`mass spectrometry. Drug Metab. Dispos. 26, 85-90 (1998).
`van Breemen RB, Tan Y, Lai J, Huang C-R, Zhao X. Immobilized thymine chromatography-
`mass spectrometry of oligonucleotides. J. Chromatogr. A. 806, 67-76 (1998).
`55. Nikolic D, van Breemen RB. Screening for inhibitors of dihydrofolate reductase using pulsed
`ultrafiltration mass spectrometry. Comb. Chem. High Throughput Screen. 1, 47-55 (1998).
`56. Chang M, Zhang F, Shen L, Pauss N, Alam I, van Breemen RB, Blond SY, Bolton JL.
`Inhibition of glutathione S-transferase activity by the quinoid metabolites of equine
`estrogens. Chem. Res. Toxicol. 11, 758-765 (1998).
`Shaw LM, Korecka M, van Breemen R, Nowak I, Brayman KL. Analysis, pharmacokinetics
`and therapeutic drug monitoring of mycophenolic acid. Clin. Biochem. 31, 323-328 (1998).
`Ito A, Shamon LA, Yu B, Mata-Greenwood E, Lee SK, van Breemen RB, Mehta RG,
`Farnsworth NR, Fong HHS, Pezzuto JM, Kinghorn AD. Antimutagenic constituents of
`Casimiroa edulis with potential cancer chemopreventive activity. J. Agric. Food Chem. 46,
`3509-3516 (1998).
`59. Chen Y, Shen L, Zhang F, Lau SS, van Breemen RB, Nikolic D, Bolton JL. The equine
`estrogen metabolite 4-hydroxyequilenin causes DNA single-strand breaks and oxidation of
`DNA bases in vitro. Chem. Res. Toxicol. 11, 1105-1111 (1998).
`60. Chung HS, Chang LC, Lee SK, Shamon LA, van Breemen RB, Mehta RG, Farnsworth NR,
`Pezzuto JM, Kinghorn AD. Flavonoid constituents of Chorizanthe diffusa with potential
`cancer chemopreventive activity. J. Agric. Food Chem. 47, 36-41 (1999).
`Fast W, Nikolic D, van Breemen R, Marletta MA, Silverman RB. Mechanistic studies of
`the inactivation of inducible nitric oxide synthase by N5-(1-iminoethyl)-L-ornithine (L-
`NIO). J. Am. Chem. Soc. 121, 903-916 (1999).
`62. Nikolic D, Fan PW, Bolton JL, van Breemen RB. Screening for xenobiotic electrophilic
`metabolites using pulsed ultrafiltration-mass spectrometry. Comb. Chem. High Throughput
`Screen. 2, 165-176 (1999).
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`63. Zhang F, Chen Y, Pisha E, Shen L, Xiong Y, van Breemen RB, Bolton JL. The major
`metabolite of equilin, 4-hydroxyequilin, autoxidizes to an o-quinone which isomerizes to
`the potent cytotoxin 4-hydroxyequilenin-o-quinone. Chem. Res. Toxicol. 12, 204-213
`(1999).
`64. Korecka M, Nikolic D, van Breemen RB, Shaw LM. The apparent inhibition of inosine
`monophosphate dehydrogenase by mycophenolic acid glucuronide is attributable to the
`presence of trace quantities of mycophenolic acid. Clin. Chem. 45, 1047-1059 (1999).
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`Fu M, Nikolic D, van Breemen RB, Silverman RB. Mechanism of inactivation of -
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`aminobutyric acid aminotransferase by (S)-4-amino-4,5-dihydro-2-thiophenecarboxylic
`acid. J. Am. Chem. Soc. 121, 7751-7759 (1999).
`66. Xu X, Wang Y, Constantinou AI, Stacewicz-Sapuntzakis M, Bowen PE, van Breemen RB.
`Solubilization and stabilization of carotenoids using micelles: Delivery of lycopene to cells
`in culture.” Lipids, 34, 1031-1036 (1999).
`67. Hadden WL, Watkins RH, Levy LW, Regalado E, Rivadeneira DM, van Breemen RB,
`Schwartz SJ. Carotenoid composition of marigold (Tagetes erecta) flower extract used as
`nutritional supplement. J. Agric. Food Chem. 47, 4189-4194 (1999).
`68. Gu C, Nikolic D, Lai J, Xu X, van Breemen RB. Assays of ligand-human serum albumin
`binding using pulsed ultrafiltration and liquid chromatography-mass spectrometry. Comb.
`Chem. High Throughput Screen. 2, 353-359 (1999).
`69. Zhang F, Fan PW, Liu X, Shen L, van Breemen RB, Bolton JL. Synthesis and reactivity of a
`potential carcinogenic metabolite of Tamoxifen: 3,4-Dihydroxytamoxifen-o-quinone. Chem.
`Res. Toxicol. 13, 53-62 (2000).
`70. Chen Y, Liu X, Pisha E, Constantinou AI, Hua Y, Shen L, van Breemen RB, Elguindi EC,
`Blond SY, Zhang F, Bolton JL. A metabolite of equine estrogens, 4-hydroxyequilenin, induces
`DNA damage and apoptosis in breast cancer cell lines. Chem. Res. Toxicol. 13, 342-350
`(2000).
`71. Nikolic D, Habibi-Goudarzi S, Corley DG, Gafner S, Pezzuto JM, van Breemen RB.
`Evaluation of cyclooxygenase-2 inhibitors using pulsed ultrafiltration-mass spectrometry.
`Anal. Chem. 72, 3853-3859 (2000).
`72. Henry LK, Puspitasari-Nienaber NL, Jarén-Galán M, van Breemen RB, Catignani G, Schwartz
`SJ. Effects of ozone and oxygen on the degradation of carotenoids in an aqueous model system.
`J. Agric. Food Chem. 48, 5008-5013 (2000).
`73. Wang Y, Xu X, van Lieshout M, West CE, Lugtenburg J, Verhoeven MA, Creemers AFL,
`Muhilal, van Breemen RB. A liquid chromatography-mass spectrometry method for the
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`quantification of bioavailability and bioconversion of -carotene to retinol in humans. Anal.
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`Chem. 72, 4999-5003 (2000).
`74. Westenburg HE, Lee KJ, Lee SK, Fong HH, van Breemen RB, Pezzuto JM, Kinghorn AD.
`Activity-guided isolation of antioxidative constituents of Cotinus coggygria. J. Nat. Prod.
`63, 1696-1698 (2000).
`75. Hamm ML, Nikolic D, van Breemen RB, Piccirilli JA. Unconventional origin of metal ion
`rescue in the hammerhead ribozyme reaction: Mn2+-assisted redox conversion of β’-
`mercaptocytidine to cytidine. J. Am. Chem. Soc. 122, 12069-12078 (2000).
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`76. Li W, Gu C, Zhang H, Awang DVC, Fitzloff JF, Fong HHS, van Breemen RB. Use of high-
`performance liquid chromatography-tandem mass spectrometry to distinguish Panax
`ginseng C. A. Meyer (Asian ginseng) and Panax quinquefolius L. (North American
`ginseng). Anal. Chem. 72, 5417-5422 (2000).
`77. Hua Y, Wainhaus SB, Yang Y, Shen L, Xiong Y, Xu X, Zhang F, Bolton JL, van Breemen
`RB. Comparison of negative and positive ion electrospray tandem mass spectrometry for
`the
`liquid chromatography
`tandem mass
`spectrometry analysis of oxidized
`deoxynucleosides. J. Am. Soc. Mass Spectrom. 12, 80-87 (2001).
`78. Chang M, Shin YG, van Breemen RB, Blond SY, Bolton JL. Structural and functional
`consequences of inactivation of human glutathione S-transferase P1-1 mediated by the
`catechol metabolite of equine estrogens, 4-hydroxyequilenin. Biochemistry, 40, 4811-4820
`(2001).
`79. Liu J, Burdette JE, Xu H, Gu C, van Breemen RB, Bhat KP, Booth N, Constantinou AI,
`Pezzuto JM, Fong HH, Farnsworth NR, Bolton JL. Evaluation of estrogenic activity of plant
`extracts for the potential treatment of menopausal symptoms. J. Agric. Food Chem. 49,
`2472-2479 (2001).
`Park EJ, Lee D, Shin YG, Lantvit DD, van Breemen RB, Kinghorn AD, Pezzuto JM.
`Analysis of 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors using liquid
`chromatography-electrospray mass spectrometry. J. Chromatogr. B Biomed. Sci. Appl. 754,
`327-32 (2001).
`van Lieshout M, West CE, Muhilal, Permaesih D, Wang Y, Xu X, van Breemen RB,
`Creemers AF, Verhoeven MA, Lugtenburg J. Bioefficacy of (cid:533)-carotene dissolved in oil
`studied in children in Indonesia. Am. J. Clin. Nutr. 73, 949-58 (2001).
`82. Mitchell DJ, Nikolic D, Rivera E, Sablin SO, Choi S, van Breemen RB, Singer TP,
`Silverman RB. Spectrometric evidence for the flavin-1-phenylcyclopropylamine inactivator
`adduct with monoamine oxidase N. Biochemistry, 40, 5447-56 (2001).
`83. Nikolic D, van Breemen RB. DNA Oxidation induced by cyclooxygenase-2. Chem. Res.
`Toxicol. 14, 351-354 (2001).
`84. Zhang F, Yao D, Hua Y, van Breemen RB, Bolton JL. Synthesis and reactivity of the
`catechol metabolites from the equine estrogen, 8,9-dehydroestrone. Chem. Res. Toxicol. 14,
`754-763 (2001).
`85. Mitchell DJ, Nikolic D, van Breemen RB, Silverman RB. Inactivation of monoamine
`oxidase B by 1-phenylcyclopropylamine: Mass spectral evidence for the flavin adduct.
`Bioorg. Med. Chem. Lett. 11, 1757-1760 (2001).
`86. Mitchell DJ, Nikolic D, Jang MH, van Breemen RB, Hille R, Silverman RB. Inactivation
`of C30A trimethylamine dehydrogenase by N-cyclopropyl-α-methylbenzylamine, 1-
`phenylcyclopropylamine, and phenylhydrazine. Biochemistry, 40, 8523-30 (2001).
`87. Wang Y, Chang WY, Prins GS, van Breemen RB. Simultaneous determination of all-trans,
`9-cis, 13-cis retinoic acid and retinol in rat prostate using liquid chromatography-mass
`spectrometry. J. Mass Spectrom. 36, 882-888 (2001).
`Johnson BM, Bolton JL, van Breemen RB. Screening botanical extracts for quinoid
`metabolites. Chem. Res. Toxicol. 14, 1546-1551 (2001).
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`88.
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`93.
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`89. Garbis SD, Melse-Boonstra A, West CE, van Breemen RB. Determination of folates in
`human plasma using hydrophilic interaction chromatography-tandem mass spectrometry.
`Anal. Chem. 73, 5358-5364 (2001).
`90. Zhang F, Swanson SM, van Breemen RB, Liu X, Yang Y, Gu C, Bolton JL. Equine
`estrogen metabolite 4-hydroxyequilenin induces DNA damage in the rat mammary tissues:
`Formation of single-strand breaks, apurinic sites, stable adducts, and oxidized bases. Chem.
`Res. Toxicol. 14, 1654-9 (2001).
`91. Yao D, Zhang F, Yu L, Yang Y, van Breemen RB, Bolton JL. Synthesis and reactivity of
`potential toxic metabolites of Tamoxifen analogues: Droloxifene and Toremifene o-
`quinones. Chem. Res. Toxicol. 14, 1643-53 (2001).
`92. Chen L, Stacewicz-Sapuntzakis M, Duncan C, Sharifi R, Ghosh L, van Breemen R, Ashton
`D, Bowen PE. Oxidative DNA damage in prostate cancer patients consuming tomato sauce-
`based entrees as a whole-food intervention. J. Natl. Cancer Inst. 93, 1872-1879 (2001).
`Shin YG, van Breemen RB. Analysis and screening of combinatorial libraries using mass
`spectrometry. Biopharmaceut. Drug Dispos. 22, 353-72 (2001).
`94. Constantinou AI, Lantvit D, Hawthorne M, Xu XY, van Breemen RB, Pezzuto JM.
`Chemopreventive effects of soy protein and purified soy isoflavones on DMBA-induced
`mammary tumors in female Sprague-Dawley rats. Nutr. Cancer, 41, 75-81 (2001).
`95. Burdette JE, Liu J, Lantvit D, Lim E, Booth N, Bhat KPL, Hedayat S, van Breemen RB,
`Constantinou AI, Pezzuto JM, Farnsworth NR, Bolton JL. Trifolium pratense (red clover)
`exhibits estrogenic effects in vivo in ovariectomized Sprague-Dawley rats. J. Nutr. 132, 27-
`30 (2002).
`Shin YG, Bolton JL, van Breemen RB. Screening drugs for metabolic stability using pulsed
`ultrafiltration mass spectrometry. Comb. Chem. High Throughput Screen. 5, 59-64 (2002).
`Papadantonakis GA, Tranter R, Brezinsky K, Yang Y, van Breemen RB, LeBreton PR.
`Low-energy, low-yield photionization, and production of 8-oxo-β’-deoxyguanosine and
`guanine from β’-deoxyguanosine. J. Phys. Chem. 106, 7704-7712 (2002).
`98. Wang Y, Catana F, Yang Y, Roderick R, van Breemen RB. An LC-MS method for
`analyzing total resveratrol in grape juice, cranberry juice, and in wine. J. Agric. Food Chem.
`50, 431-5 (2002).
`van Breemen RB, Xu X, Viana MA, Chen L, Stacewicz-Sapuntzakis M, Duncan C, Bowen
`PE, Sharifi R. Liquid chromatography-mass spectrometry of cis- and all-trans-lycopene in
`human serum and prostate tissue after dietary supplementation with tomato sauce. J. Agric.
`Food Chem. 50, 2214-9 (2002).
`100. Liu X, Yao J, Pisha E, Yang Y, Hua Y, van Breemen RB, Bolton JL. Oxidative DNA damage
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`96.
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`97.
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`99.
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`induced by equine estrogen metabolites: Role of estrogen receptor-. Chem. Res. Toxicol. 15,
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`512-9 (2002).
`101. Constantinou AI, Lucas LM, Lantvit D, Hawthorne M, Xu X, Nho CW, Jeffery EH, Christov
`K, van Breemen RB, Pezzuto JM. Soy protein isolate prevents chemically-induced rat
`mammary tumors. Pharmaceut. Biol. 40(Supplement), 24-34 (2002). *This paper was cited
`by the NIH in the Annual Bibliography of Significant Advances in Dietary Supplement
`Research, 2002. Ed. by RB Costello and LG Saldanha. NIH Publication No. 04-5450,
`September 2006.
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`102. Johnson BM, Nikolic D, van Breemen RB. Applications of pulsed ultrafiltration-mass
`spectrometry. Mass Spectrom. Rev. 21, 76-86 (2002).
`103. Li W, Sun Y, Fitzloff JF, van Breemen RB. Evaluation of commercial ginkgo and Echinacea
`dietary supplements for colchicines using liquid chromatography-tandem mass spectrometry.
`Chem. Res. Toxicol. 15, 1174-8 (2002).
`104. Prins GS, Chang WY, Wang Y, van Breemen RB. Retinoic acid receptors and retinoids are
`up-regulated in the developing and adult rat prostate by neonatal estrogen exposure.
`Endocrinology, 143, 3628-40 (2002).
`105. Bowen PE, Chen LW, Stacewicz-Sapuntzakis M, Duncan C, Sharifi R, Ghosh L, Kim HS,
`Christov-Tzelkov K, van Breemen RB. Tomato sauce supplementation and prostate cancer:
`Lycopene accumulation and modulation of biomarkers of carcinogenesis. Exp. Biol. Med. 227,
`886-893 (2002).
`106. Yu C, Shin YG, Chow A, Li Y, Kosmeder JW, Lee YS, Hirschelman WH, Pezzuto JM, Mehta
`RG, van Breemen RB. Human, rat, and mouse metabolism of resveratrol. Pharm. Res. 19,
`1907-14 (2002).
`107. Burdette JE, Chen SN, Lu ZZ, Xu H, White BEP, Fabricant DS, Liu J, Fong HH, Farnsworth
`NR, Constantinou AI, van Breemen, RB, Pezzuto JM, Bolton JL. Black cohosh (Cimicifuga
`racemosa L.) protects against menadione-induced DNA damage through scavenging of
`reactive oxygen species: bioassay-directed isolation and characterization of active principles.
`J. Agric. Food Chem. 50, 7022-8 (2002).
`108. Yang Y, Nikolic D, Swanson SM, van Breemen RB. Quantitative determination of N7-
`methyldeoxyguanosine and O6-methyldeoxyguanosine in DNA by LC-UV-MS-MS. Anal.
`Chem. 74, 5376-5382 (2002).
`109. Yu C, Shin YG, Kosmeder
`JM, van Breemen RB. Liquid
`JW, Pezzuto
`chromatography/tandem mass spectrometric determination of inhibition of human cytochrome
`P450 isozymes by resveratrol and resveratrol-3-sulfate. Rapid Commun. Mass Spectrom. 17,
`307-313 (2003).
`110. van Lieshout M, West CE, van Breemen RB. Isotopic tracer techniques for studying the
`bioavailability and bioefficacy of dietary carotenoids, particularly (cid:533)-carotene, in humans: a
`review. Am. J. Clin. Nutr. 77, 12-28 (2003).
`111. Li W, Sun Y, Joseph J, Fitzloff JF, Fong HHS, van Breemen RB. p-Hydroxybenzoic acid alkyl
`esters in Andrographis paniculata herbs, commercial extracts, and formulated products. J.
`Agric. Food Chem. 51, 524-529 (2003).
`112. Fang L, Pajkovic N, Wang Y, Gu C, van Breemen RB. Quantitative analysis of lycopene
`isomers in human plasma using high-performance liquid chromatography-tandem mass
`spectrometry. Anal. Chem. 75, 812-817 (2003).
`113. Su B-N, Park EJ, Nikolic D, Santarsiero BD, Mesecar AD, Vigo JS, Graham JG, Cabieses
`F, van Breemen RB, Fong HSS, Farnsworth NR, Pezzuto JM, Kinghorn AD. Activity-
`guided isolation of novel norwithanolides from Deprea subtriflora with potential cancer
`chemopreventive activity. J. Org. Chem. 68, 2350-2361 (2003).
`114. Su B-N, Cuendet M, Nikolic D, Kristinsson H, Ingolfsdottir K, van Breemen RB, Fong HHS,
`Pezzuto JM, Kinghorn AD. NMR study of fumarprotocetraric acid, a complex lichen
`depsidone derivative from Cladonia furcata. Magn. Reson. Chem. 41, 391-394 (2003).
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`115. Gu J-Q, Li W, Kang Y-H, Su B-N, Fong HHS, van Breemen RB, Pezzuto JM, Kinghorn AD.
`Minor withanolides from Physalis philadelphica: Structures, quinone reductase induction
`activities, and liquid chromatography (LC)-MS-MS investigation of artifacts. Chem. Pharm.
`Bull. (Tokyo), 51, 530-539 (2003).
`116. Yao J, Li Y, Chang M, Wu H, Yang X, Goodman JE, Liu X, Liu H, Mesecar AD, van Breemen
`RB, Yager JD, Bolton JL. Catechol estrogen 4-hydroxyequilenin is a substrate and an inhibitor
`of catechol-O-methyltransferase. Chem. Res. Toxicol. 16, 668-675 (2003).
`117. Li W, Sun Y, Liang W, Fitzloff JF, van Breemen RB. Identification of caffeic acid derivatives
`in Actea racemosa (Cimicifuga racemosa, black cohosh) by liquid chromatography/tandem
`mass spectrometry. Rapid Commun. Mass Spectrom. 17, 978-982 (2003).
`118. Johnson BM, Qiu S-X, Zhang S, Zhang F, Burdette JE, Yu L, Bolton JL, van Breemen RB.
`Identification of novel electrophilic mammalian metabolites of Piper methysticum Forst.
`(Kava). Chem. Res. Toxicol. 16, 733-740 (2003).
`119. Liu X, Zhang F, Liu H, Burdett JE, Li Y, Overk CR, Pisha E, Yao J, van Breemen RB,
`Swanson SM, Bolton JL. Effect of halogenated substituents on the metabolism and
`estrogenic effects of the equine estrogen, equilenin. Chem. Res Toxicol. 16, 741-749 (2003).
`120. Kouloumbos VN, Tsipi DF, Hiskia AE, Nikolic D, van Breemen RB. Identification of
`photocatalytic degradation products of diazinon in TiO2 aqueous suspensions using
`GC/MS/MS and LC/MS with quadrupole time-of-flight mass spectrometry. J. Am. Soc.
`Mass Spectrom. 14, 803-817 (2003).
`121. Su B-N, Park EJ, Nikolic D, Vigo JS, Graham JG, Cabieses F, van Breemen RB, Fong HHS,
`Farnsworth NR, Pezzuto JP, Kinghorn AD. Isolation and characterization of miscellaneous
`secondary metabolites of Deprea subtriflora. J. Nat. Prod. 66, 1089-1093 (2003).
`122. Cheng X, Shin YG, Levine BS, Smith AC, Tomaszewski JE, van Breemen RB. Quantitative
`analysis of betulinic acid in mouse, rat and dog plasma using electrospray LC-MS. Rapid
`Commun. Mass Spectrom. 17, 2089-2092 (2003).
`123. Johnson BM, van Breemen RB. In vitro formation of quinoid metabolites of the dietary
`supplement Cimicifuga racemosa (black cohosh). Chem. Res. Toxicol. 16, 838-846 (2003).
`124. van Lieshout M, West CE, van de Bovenkamp P, Wang Y, Sun Y, van Breemen RB, M