Page 1 of 2
`
`Description
`
`Designation: [CCEB 481, MDB strain BU 277, NCIB 8295, NCPPB 1965, NCTC 10332, NRRL B771, R. Hugh
`815]
`Deposited Name: Pseudomonas aeruginosa (Schroeter) Migula
`Product Description: Type strain. Positive control for molecular detection in bioaerosols. Quality control
`strain for API and Sensititre products. Used in bacterial resistance testing of adhesives and paint.
`
`Propagation
`
`Medium
`ATCC® Medium 3: Nutrient agar or nutrient broth
`
`Growth Conditions
`Temperature: 37°C
`Atmosphere: Aerobic
`
`Propagation Procedure
`1. Open vial according to enclosed instructions.
`2. From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0
`mL pipette and use to rehydrate the entire pellet.
`3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
`4. Use several drops of this suspension to inoculate an additional broth tube, a #3 agar slant and/or a
`plate.
`5. Incubate the tubes and plate at 37°C for 24 hours.
`
`
`
`Notes
`
`This strain produces two types of diffusible pigment that may mask each other:
`1. Fluorescein: diffusible, green or yellowgreen in color, and exhibits fluorescence with shortwave UV
`light (254 nm). Production is enhanced by growth in an irondeficient medium.
`2. Pyocyanine: diffusible, blue, a chloroformsoluble pigment characteristic of Pseudomonas aeruginosa.
`Incubate in the dark as broadspectrum light may inactivate this pigment.
`
`
`The basic requirements for pigment production are not well known, and thus color production by the
`pseudomonads has been an erratic property that may be lost with repeated subculturing. However, this strain
`has remained stable.
`Additional information on this culture is available on the ATCC® web site at www.atcc.org.
`
`References
`
`References and other information relating to this product are available online at www.atcc.org.
`
`Biosafety Level: 2
`
`Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in
`the current publication of the Biosafety in Microbiological and Biomedical Laboratories from the U.S.
`Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes
`for Health.
`
`ATCC Warranty
`
`The viability of ATCC® products is warranted for 30 days from the date of shipment, and is valid only if the
`product is stored and cultured according to the information included on this product information sheet. ATCC
`lists the media formulation that has been found to be effective for this strain. While other, unspecified media
`may also produce satisfactory results, a change in media or the absence of an additive from the ATCC
`recommended media may affect recovery, growth and/or function of this strain. If an alternative medium
`formulation is used, the ATCC warranty for viability is no longer valid.
`
`Disclaimers
`
`This product is intended for laboratory research purposes only. It is not intended for use in humans.
`While ATCC uses reasonable efforts to include accurate and uptodate information on this product
`
`Product Sheet
`Pseudomonas aeruginosa
`(ATCC® 10145™)
`
`Please read this FIRST
`
`Storage Temp.
`Frozen: 80°C or
`colder
`FreezeDried: 2°C
`to 8°C
`Live Culture: See
`Propagation
`Section
`
`Biosafety Level
`2
`
`
`
`
`
`Intended Use
`
`This product is intended for research use only. It is not
`intended for any animal or human therapeutic or
`diagnostic use.
`
`Citation of Strain
`
`If use of this culture results in a scientific publication, it
`should be cited in that manuscript in the following
`manner: Pseudomonas aeruginosa
`(ATCC® 10145™)
`
`American Type Culture Collection
`PO Box 1549
`Manassas, VA 20108 USA
`www.atcc.org
`
`800.638.6597 or 703.365.2700
`Fax: 703.365.2750
`Email: Tech@atcc.org
`
`Or contact your local distributor
`
`PARAGON - EXHIBIT 2027
`
`
`
`Description
`
`Designation: [CCEB 481, MDB strain BU 277, NCIB 8295, NCPPB 1965, NCTC 10332, NRRL B771, R. Hugh
`815]
`Deposited Name: Pseudomonas aeruginosa (Schroeter) Migula
`Product Description: Type strain. Positive control for molecular detection in bioaerosols. Quality control
`strain for API and Sensititre products. Used in bacterial resistance testing of adhesives and paint.
`
`Propagation
`
`Medium
`ATCC® Medium 3: Nutrient agar or nutrient broth
`
`Growth Conditions
`Temperature: 37°C
`Atmosphere: Aerobic
`
`Propagation Procedure
`1. Open vial according to enclosed instructions.
`2. From a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0
`mL pipette and use to rehydrate the entire pellet.
`3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
`4. Use several drops of this suspension to inoculate an additional broth tube, a #3 agar slant and/or a
`plate.
`5. Incubate the tubes and plate at 37°C for 24 hours.
`
`
`
`Notes
`
`This strain produces two types of diffusible pigment that may mask each other:
`1. Fluorescein: diffusible, green or yellowgreen in color, and exhibits fluorescence with shortwave UV
`light (254 nm). Production is enhanced by growth in an irondeficient medium.
`2. Pyocyanine: diffusible, blue, a chloroformsoluble pigment characteristic of Pseudomonas aeruginosa.
`Incubate in the dark as broadspectrum light may inactivate this pigment.
`
`
`The basic requirements for pigment production are not well known, and thus color production by the
`pseudomonads has been an erratic property that may be lost with repeated subculturing. However, this strain
`has remained stable.
`Additional information on this culture is available on the ATCC® web site at www.atcc.org.
`
`References
`
`References and other information relating to this product are available online at www.atcc.org.
`
`Biosafety Level: 2
`
`Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in
`the current publication of the Biosafety in Microbiological and Biomedical Laboratories from the U.S.
`Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes
`for Health.
`
`ATCC Warranty
`
`The viability of ATCC® products is warranted for 30 days from the date of shipment, and is valid only if the
`product is stored and cultured according to the information included on this product information sheet. ATCC
`lists the media formulation that has been found to be effective for this strain. While other, unspecified media
`may also produce satisfactory results, a change in media or the absence of an additive from the ATCC
`recommended media may affect recovery, growth and/or function of this strain. If an alternative medium
`formulation is used, the ATCC warranty for viability is no longer valid.
`
`Disclaimers
`
`This product is intended for laboratory research purposes only. It is not intended for use in humans.
`While ATCC uses reasonable efforts to include accurate and uptodate information on this product
`
`Product Sheet
`Pseudomonas aeruginosa
`(ATCC® 10145™)
`
`Please read this FIRST
`
`Storage Temp.
`Frozen: 80°C or
`colder
`FreezeDried: 2°C
`to 8°C
`Live Culture: See
`Propagation
`Section
`
`Biosafety Level
`2
`
`
`
`
`
`Intended Use
`
`This product is intended for research use only. It is not
`intended for any animal or human therapeutic or
`diagnostic use.
`
`Citation of Strain
`
`If use of this culture results in a scientific publication, it
`should be cited in that manuscript in the following
`manner: Pseudomonas aeruginosa
`(ATCC® 10145™)
`
`American Type Culture Collection
`PO Box 1549
`Manassas, VA 20108 USA
`www.atcc.org
`
`800.638.6597 or 703.365.2700
`Fax: 703.365.2750
`Email: Tech@atcc.org
`
`Or contact your local distributor
`
`PARAGON - EXHIBIT 2027
`
`
`
`Page 2 of 2
`
`sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature
`and patents are provided for informational purposes only. ATCC does not warrant that such information has
`been confirmed to be accurate.
`This product is sent with the condition that you are responsible for its safe storage, handling, and use.
`ATCC is not liable for any damages or injuries arising from receipt and/or use of this product. While
`reasonable effort is made to ensure authenticity and reliability of strains on deposit, ATCC is not liable for
`damages arising from the misidentification or misrepresentation of cultures.
`Please see the enclosed Material Transfer Agreement (MTA) for further details regarding the use of this
`product. The MTA is also available on our Web site at www.atcc.org
`
`Additional information on this culture is available on the ATCC web site at www.atcc.org.
`© ATCC 2015. All rights reserved. ATCC is a registered trademark of the American Type Culture Collection. [06/05]
`
`Product Sheet
`Pseudomonas aeruginosa
`(ATCC® 10145™)
`
`Please read this FIRST
`
`Storage Temp.
`Frozen: 80°C or
`colder
`FreezeDried: 2°C
`to 8°C
`Live Culture: See
`Propagation
`Section
`
`Biosafety Level
`2
`
`
`
`
`
`Intended Use
`
`This product is intended for research use only. It is not
`intended for any animal or human therapeutic or
`diagnostic use.
`
`Citation of Strain
`
`If use of this culture results in a scientific publication, it
`should be cited in that manuscript in the following
`manner: Pseudomonas aeruginosa
`(ATCC® 10145™)
`
`American Type Culture Collection
`PO Box 1549
`Manassas, VA 20108 USA
`www.atcc.org
`
`800.638.6597 or 703.365.2700
`Fax: 703.365.2750
`Email: Tech@atcc.org
`
`Or contact your local distributor
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