`
`Description
`
`Designation: Marburg strain [ATCC 6051U, CCM 2216, CCRC 10255, CCUG 163B, CFBP 4228, CIP 52.65,
`DSM 10, IAM 12118, IFO 12210, IFO 13719, IFO 16412, IMET 10758, JCM 1465, LMG 7135, NCAIM B.01095,
`NCCB 32009, NCCB 53016, NCCB 70064, NCFB 1769, NCIB 3610, NCTC 3610, NRRL B4219, NRS 1315, NRS
`744, VKM B501]
`Deposited Name: Bacillus subtilis (Ehrenberg) Cohn
`Product Description: Type strain. Bacteriophage host.
`
`Propagation
`
`Medium
`ATCC® Medium 3: Nutrient agar or nutrient broth
`
`Growth Conditions
`Temperature: 30°C
`Atmosphere: Aerobic
`
`Propagation Procedure
`1. Open the freezedried vial according to enclosed instructions.
`2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or
`1.0 mL pipette. Rehydrate the entire pellet.
`3. Aseptically transfer this aliquot back into the broth tube. Mix well.
`4. Use several drops of the suspension to inoculate a #3 slant, and/or plate.
`5. Incubate all tubes and plates at 30°C for 24 hours.
`
`
`
`Notes
`
`On #3 agar two colony types are observed. 1) Circular, entire, low convex, shiny and opaque. 2) Irregular,
`rough, flat and opaque. The two colony types by 16S sequencing are identical genus and species. Growth
`is observed after 24 hours. Growth may be poor in broth incubated statically, but heavy on agar.
`This strain has been cited for use in bacterial resistance testing of latex paint (Machemer WE, 1979), blood
`screening for phenylketonuria (Guthrie R, 1961), and accumulation of metal ions from aqueous solutions
`(Brierley JA et al., 1990). This strain has also been cited as able to produce isoprene (Kuzma J et al., 1995).
`Available as Uniplus™ ATCC® 6051U™
`Additional information on this culture is available on the ATCC® web site at www.atcc.org.
`
`References
`
`References and other information relating to this product are available online at www.atcc.org.
`
`Biosafety Level: 1
`
`Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in
`the current publication of the Biosafety in Microbiological and Biomedical Laboratories from the U.S.
`Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes
`for Health.
`
`ATCC Warranty
`
`The viability of ATCC® products is warranted for 30 days from the date of shipment, and is valid only if the
`product is stored and cultured according to the information included on this product information sheet. ATCC
`lists the media formulation that has been found to be effective for this strain. While other, unspecified media
`may also produce satisfactory results, a change in media or the absence of an additive from the ATCC
`recommended media may affect recovery, growth and/or function of this strain. If an alternative medium
`formulation is used, the ATCC warranty for viability is no longer valid.
`
`Disclaimers
`
`This product is intended for laboratory research purposes only. It is not intended for use in humans.
`While ATCC uses reasonable efforts to include accurate and uptodate information on this product
`sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature
`and patents are provided for informational purposes only. ATCC does not warrant that such information has
`
`Product Sheet
`Bacillus subtilis subsp.
`subtilis (ATCC® 6051™)
`
`Please read this FIRST
`
`Storage Temp.
`Frozen: 80°C or
`colder
`FreezeDried: 2°C
`to 8°C
`Live Culture: See
`Propagation
`Section
`
`Biosafety Level
`1
`
`
`
`
`
`Intended Use
`
`This product is intended for research use only. It is not
`intended for any animal or human therapeutic or
`diagnostic use.
`
`Citation of Strain
`
`If use of this culture results in a scientific publication, it
`should be cited in that manuscript in the following
`manner: Bacillus subtilis subsp. subtilis
`(ATCC® 6051™)
`
`American Type Culture Collection
`PO Box 1549
`Manassas, VA 20108 USA
`www.atcc.org
`
`800.638.6597 or 703.365.2700
`Fax: 703.365.2750
`Email: Tech@atcc.org
`
`Or contact your local distributor
`
`PARAGON - EXHIBIT 2025
`
`
`
`Page 2 of 2
`
`been confirmed to be accurate.
`This product is sent with the condition that you are responsible for its safe storage, handling, and use.
`ATCC is not liable for any damages or injuries arising from receipt and/or use of this product. While
`reasonable effort is made to ensure authenticity and reliability of strains on deposit, ATCC is not liable for
`damages arising from the misidentification or misrepresentation of cultures.
`Please see the enclosed Material Transfer Agreement (MTA) for further details regarding the use of this
`product. The MTA is also available on our Web site at www.atcc.org
`
`Additional information on this culture is available on the ATCC web site at www.atcc.org.
`© ATCC 2013. All rights reserved. ATCC is a registered trademark of the American Type Culture Collection. [08/02]
`
`Product Sheet
`Bacillus subtilis subsp.
`subtilis (ATCC® 6051™)
`
`Please read this FIRST
`
`Storage Temp.
`Frozen: 80°C or
`colder
`FreezeDried: 2°C
`to 8°C
`Live Culture: See
`Propagation
`Section
`
`Biosafety Level
`1
`
`
`
`
`
`Intended Use
`
`This product is intended for research use only. It is not
`intended for any animal or human therapeutic or
`diagnostic use.
`
`Citation of Strain
`
`If use of this culture results in a scientific publication, it
`should be cited in that manuscript in the following
`manner: Bacillus subtilis subsp. subtilis
`(ATCC® 6051™)
`
`American Type Culture Collection
`PO Box 1549
`Manassas, VA 20108 USA
`www.atcc.org
`
`800.638.6597 or 703.365.2700
`Fax: 703.365.2750
`Email: Tech@atcc.org
`
`Or contact your local distributor