`
`A-498 ATCC ® HTB-44 ™ Homo sapiens kidney carcinoma
`
`A-498 (ATCC® HTB-44 TM)
`Organism: fiQmQ...$apiens,..llli!nJill I Tissue: kidney Disease: carcinoma
`
`GENERAL INFORMATION
`
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`CULTURE ME1'HCD
`
`SPE.C!HCATION'S I H~STORY I DOC! j~1f.:NTA.TH)N
`
`- ,
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`·1
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`:
`I
`:
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`Permits and Restrictions
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`Vlow Porrnits
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`Organism
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`Tissue
`
`Product Format
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`Homo sapiens, human
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`kldnoy
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`frozen
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`Morphology
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`epithelial
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`Culture Properties
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`adherent
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`Blosafety Level
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`Biosafety classlflca/1011 Is based 011 U.S. Pubflc Health Service
`~ .. It is the responslbflity of tho customer to ensure that
`thoir facilities comply with blosafety regulations for their own
`
`Disease
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`Age
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`Gender
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`country.
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`carcinoma
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`52 years
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`female
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`Storage Conditions
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`liquid nitrogen vapor phase
`
`h tps://www.atcc.org/Products/AII/HTB-44.aspx?&p=1 &rel=generalinformatlon#generalinformation
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`A-498 ATCC ® HTB-44 TM Homo sapiens kidney carcinoma
`
`A-498 (ATCC® HTB-44 ™)
`Organism: Homo sa~,J:u!Jru!n I Tissue: .!ill!ru:!Y / Disease: carcinoma
`
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`Derivation
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`Clinical Data
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`Tumorlgenlc
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`Effects
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`Commonts
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`S. Aaronson isolated this line using techniques as described for
`ATCC HTB-41.
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`ferna!e
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`Yos
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`Yes, in nude mice; forms undifferentiated carcinoma; also forms
`tumors In anti thymocyte serum treatBd newborn mice
`
`https:/ /www.alee.org/Products/ All/HT B-44. aspx? &p= 1 &rel =characteristics#characteristics
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`A-498 ATCC ® HTB-44 rM Homo sapiens kidney carcinoma
`
`A-498 (ATCC® HTB-44™)
`Organism: ~IUQl!.£,..hllinJ!n I Tissue: .!lli!rulY. I Disease: carcinoma
`' CHARACT[R,:H!Ui ! CULTURE METHOD
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`r
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`l
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`{iPECIFICATONS
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`Complete Growth Medium
`
`The base medium for this cell lino is ATCC-formulated Eagle's
`Minimum Essential Medium, Catalog No. 30-2003. To make the
`complete growth medium, add !11<, following components to the
`base medium: fetal bovine serum to a final concentration of 10%.
`
`Subculturing
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`1. Remove and discard culture medium.
`
`2. Briefiy rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM
`EDTA solution to removo all traces of serum which contains
`trypsin inhibitor.
`
`3. Add 2.0 to 3.0 mL ofTrypsin-EDTA solution to fiask and
`observe cells under an Inverted microscopH until cell layer is
`dispersed (usually within 5 to Hi minutes).
`Note: To avoid clumping do 1101 agitate the cells by hitting or
`shaking the, fiask while waiting for the cells to detach. Cells
`that are difficult to detach may be placed at 37'C to facilitate
`dispersal.
`
`4. Add 6.0 to 8.0 ml of complete growth medium and aspirate
`cells by gently pipetting.
`
`5. Add appropriate aliquots of the cell suspension to new culture
`vessels.
`
`6. Incubate culturns at 37'C.
`
`Subcultivatlon Ratio: A subcullivatlon ratio of 1 :3 to 1 :8 Is
`recommended
`Medium Renewal: Twice per week
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`Cryopreservatlon
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`Freeze medium: culture rnediurn, 95%; DMSO, 5%
`Storage temperature: liquid nitrogen vapor phase
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`Culture Conditions
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`Atmosphere: air, 95%; carbon dioxide (CO2), 5%
`Temperature: 37'C
`
`https://www.atcc.org/Products/AII/HTB-44 .aspx?&p=1&rel=cu l1uremethod#culturemethod
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`6/20/20 18
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`A-498 ATCC ® HTB-44 rM Homo sapiens kidney carcinoma
`
`A-498 (ATCC® HTB-44 TM)
`Organism: /jQJJJ_Q_J,apiens, human / Tissue: kidney / Disease: carcinoma
`
`SPECIFICATIONS
`
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`STR Profllo
`
`lsoenzymes
`
`Amologonin: X
`CSF1PO: 1·1,12
`D13S317: 12
`D168539: 12
`D5S818: 11 ,13
`D7S820: 10,11
`TH01: 6,9.3
`TPOX: 8,11
`vWA: 18
`
`AK-1, 1
`ES-D, 2
`G6PD, B
`GL0-I,2
`Mo-2, 1
`PGM1, 1-2
`PGM3, 1
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`https://www.atcc.org/Products/AII/HTB-44.aspx?&p=1&rel=specifica!ions#specifications
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`Breckenridge Exhibit 1156
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`HTB-44-A498
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`6/20/2018
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`A-498 ATCC ® HTB-44 TM Homo sapiens kidney carcinoma
`
`A-498 (ATCC® HTB-44™)
`
`Organism: Homo sapiens, human / Tissue: ~Y / Disease: carcinoma
`
`I ,;ENEfl'\' 1NfCF.C1{,7' C':
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`Namo of Depositor
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`W Nelson-Floes
`
`Doposited As
`
`iiomo sapiens
`
`SPFC!!"' !C.\Tln\;B
`
`HISTORY
`
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`I
`
`References
`
`Fogh J, et al. Absence of Hela cell contamination In 169 cell lines
`derived from human tumors. J. Natl. Cancer Inst. 58: 209-214,
`1977. PubMed: ~
`
`Goodfellow M, et al. One hundred and twcmty-soven cultured
`human tumor cell lines producing tumors in nude mice. J. Natl.
`
`Cancer Inst. 59: 221-226, 1977. PubMed: I7210034
`
`Faust JB, Meokor TC. Amplification and oxprossion ot tho bcl-1
`
`gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463,
`
`1992. PubMed: 1568216
`
`Giard DJ, et al. 1n vitro cultivation of human tumors:
`establishment of cell lines derived from a series of solid tumors. J.
`Nall. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
`
`Fonh J. Cultivation, characterization, and identification of human
`tumor cells wlt11 emphasis on kidney, testis, and bladder tumors.
`Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
`
`htlps://www.atcc.org/Products/AII/HTB-44.aspx?&p=1 &rel=history#history
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`1/1
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`Breckenridge Exhibit 1156
`Breckenridge v. Novartis IPR2017-01592
`HTB-44-A498
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`A-498 ATCC ® HTB-44 TM Homo sapiens kidney carcinoma
`
`A-498 (ATCC® HTB-44 ™)
`Organism: ~~,..hilln.an / Tissue: !ill!ru!Y. / Disease: carcinoma
`
`Permits
`
`Theso permits may bo required for shipping this product:
`
`DOCUMENTATION
`
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`
`Basic Documentation
`
`References
`
`CustorrMrs located in the stat<1 of Hawaii wlll neod to contact
`t11e Hawaii Depa11ment of Agriculture to determine if an Import
`Permit is required. A copy of the permit or documentation that
`a permit is not required must be sent to ATCC in advance of
`sl1ipment.
`
`8
`
`Product Sheet Certificate of Analysis
`
`SDS
`
`Fogh J, et al. Absence of Hela cell contamination In 169 cell lines
`derived frorn hurnan tumors. J. Natl. Cancer Inst. 58: 209 .. 214,
`1977. PubMod: 833871
`
`Goodfollow M, et al. Orlfl hundred and twenty-soven cultured
`
`human tumor coll lines producing tumors in nude mice. ,I. Natl.
`
`Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
`
`Faust Jl3, Meeker TC. Amplification and expression of the bcl-1
`
`gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463,
`
`1992. PubMed: 1568216
`
`Giard DJ, et al. In vitro cultivation of human tumors:
`establishment of cell lines derived from a series of solid tumors. J.
`Natl. Cancer Inst. 51: 1417-1423 , 1973, PubMed: 4357758
`
`Fogh J. Cultivation, characterization, and identification of human
`tumor cells witt, emµhasis on kidney, testis, and bladder tumors.
`
`Natl. Cancer Inst. Monogr. 4fJ· fj.,9, 1978. PubMe,J: 571047
`
`https://www.atcc.org/Products/AII/HTB-44.aspx?&p=1&rel=documentation#documentation
`
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