Figure 1 A
`
`Blechooes
`
`
`
`voor come,SPRLTF‘i Fetterre
`
`Eleciodes
`
`i&
`
`
`
`
`Feng
`Cargmnia
`Bato
`Digg
`
`
`
`ERE
`theretenctatieetien
`ah
`AN
`NN
`\
`“
`4
`h
`A
`D
`Q
`O
`C
`:.
`Pat
`Q
`3
`e
`0
`0
`8
`
`8
`re
`ms
`
`6 E
`
`Oc/L
`
`lectroces,
`
` Preto
`
` TRINEToenh
`
`Ceram
`
`Pare
`Ceram
`
`
`
`
`
`8 © 8
`
`
`
`
`b50- 3009
`
`

`

`
`
`

`

`Figure 1 C
`
`GenePrinting and NanoReactor
`Technology
`
`inkjet DNAprinting
`
`*High throughput
`
`+ Low errorrate
`
`“Si nanoreactor gene assembly
`+ Pracisecontrol
`
`“Low reagent use
`
`Inkjet
`
`Silicon
`
`Nanoreactor
`
`
`
`Synthesis
`
`Assembly
`
`Reagents
`|
`
`OcC/Ee
`
`
`
`
`CycleTemperature
`Nanoreactor
`
`2Kbase gene
`g
`
`

`

`Figure? A
`
`Process FlowChart
`
`> Cloning necessary Oc/P
`
`* Allcorrect (Le, additional
`relatedvariants mayb¢
`present}
`
`Optionsfor sequence
`accuracy:
`
`> Nocloningnecessary
`
`* Only correct (no
`additional variants present}
`
`

`

`© 13k
`«Linear

`
`All Correct
`
`<1 is 10kberror OC/G
`
`

`

`4-3kb
`+
`» Plasmid
`
`© <7 in 100K) error o</9
`
`

`

`Figure 3
`
`Oligo Printer
`
`ha A
`NET
`Ban fom
`esténtion
`Acetamtnis, Ne
`
`a
`NSmee
`
`Waste Sak ‘i
`
`
`Oocle
`
`
`
`
`
`
`At oa
`SaRey
`
`,_
`
`
`
`
`
`SEF
`Seed,
`Pie Rete Se
`ake
`Mat
`SAR Sa CRA ee Doe eb ey an
`Osiginer De-block, Acetaritris AZ
`
`om,
`48 fmm
`apt moees,
`Wate
`
`

`

`Oligo Wafer Reactor
`
` ASO yr
`
`Reactors arrayed
`on L5mmpitch
`
`. |
`
`:
`
`:
`
`“Ny
`
`|
`
`CN A
`
`SONS
`
`WY
`
`RRARcsGi RR RETTeLAARN
`
`Crags
`
`Section
`
`ess
`-:
`::=
`s;:
`§x
`
`&s 4i$,*\4
`
`\ |
`
` ae
`
`wt"att
`ae
`
`wrPo
`wt
`ro
`aaa » ANA
`nS
`
`FunctionallyLlActiveArea
`
`\
`
`7 )
`
`SHna
`
`

`

`ron
`nto h
`
`|
`ign's
`
`0
`urface energy m
`
`
`
`
`

`

`Figure 6A
`
`BEd
`
`ree,
`
`*
`
`
`etreaee
`rteBeiner
`
`
`
`10/30
`
`
`
`

`

`a
`
`|
`I
`
`Figure 6B
`
`Aanquae, eobreiens Laneatt
`
`po|I
`
`oo0opoo00
`
`aoponan
`
`
`-AogoocudogggSSS)aSeeepooaaSeeSo0eboqg,
`-boo.a=weetHifi<=[=>
`
`obeeegbegggaeeacoaSSS
`oooompooooaSESS
`
`
`f
`
`/
`
`Sate«ceoPSY
`eee”
`
`NA(EPPPEPESEPODEDPEDOE*toSe~~
`
`EFABatBalBYBivee
`
`aa\Sima
`
`
`
`
`
`
`
`
`
`{EEE.
`
`
`
`
`
`
`
`
`
`weoe
`
` WBSs.aensAena
`
`11/30
`
`Ba
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`

`

`Figure 6C
`
`12/30
`
`

`

`13/30 =aedl
`
`fon
`
`Figure 7
`
`ae
`
`

`

`Figure 8 Of/FTL
`
`

`

`Flowcell Open
`
`FlowcellClosed
`
`Figure 9
`Waste Collector
`Flowcell Bod
`
`Of/GL
`
`~<—
`
`

`

`
`
`Figure 10
`
`Single Groove Vacuum Chuck
`
`
`Sintered Metal
`
`
`
`Receiving
`Thanmaliantel
`
`CA
`
`
`
`q
`
`b
`
`OC/9L
`
`Sintered Metal Chuck Cross Section
`
` Sonn Wafer
`Tsae 7 é
`“\.
`
`
`
`
`
`Vacant|oS\
`
`
`
`So
`
`Sintered Metal Insert
`at
`id
`La
`gre
`* Fined in Place with
`Adheshs
`
`“HoceivingLlement and
`Thermal Contrat
`
`

`

`OC/sAL
`
`
`
`‘ \\\)s
`
`Figure 11
`
`ok
`
`ARUN
`
`

`

`Figure 12
`
`Polymerase Chain Assembly
`
`OcC/SL
`
`Oligo poo!
`
`poycel
`
`yoyces
`Desired construct
`
`

`

`Figure 13
`
`Advantage of Long Oligos
`
`Short oligo (50bp}
`
`Longoligo (300bp)
`
`AAA
`
`SARA
`
` .

`
`Me
`‘
`
`SARA
`
` SEAR)
`
`SERRA
`
`
`AMAR
`
`
`
` ANT
`NARAi
`:
`ee ky GG Gg aa
`Por
`hoy
`
`AN‘
`
`i
`
`
`
`
` SE
`
`Oc/6GL
`
`No incorrect hyoridization possible
`
`apssembly
`
`AAA
`NAAN
`
`
`SAAN
`RANANNNARAANARANNAN
`
`SANNA,
`SANNA,
`
`Perfect construct
`
` +
`
`e
`
`+
`
`“
`
`Incorrect hybridization
`
`Te
`
`Tse
`
`HOA
`AAT
`
`
`SAANNAAANARNIAANAARRARAANY
`AMAA
`
`
`
`ROI
`SANAAAAARAANAAAAAAARARA,
`
`Incorrect construct
`
`
`
`RARE
`AA
`
`
`

`

`Figure 14
`
`PCA + Gibson: 2-10Kb assembly
`
` y
`
`ANASANE
`
`
`
`PCA SONA
`
`
`
`O0c/O02
`
`PCA
`
`ALINEEANENERRNNM
`
`
`
`SRR
`
`
`
`2-10kb
`{Gibson Assembly
`
`
`

`

`Of/LZ
`
`Error Correction: High Error Rate
`
`Mutation
`
`a
`
`re-hybridization
`
`Cenatureand
`
`\utetion cleaving
`| and chew backerror
`
`
`
`

`

`Figure 16
`
`
`
`yorrection: Low Error Rate Error(
`
`
`
`RSs pes
`Mutation
`
`ye
`
`ty Denature and
`
`
`
`re-hyridization
`
`
`
`
`
`Of/2S
`
`
`
`| Mutation
`| Cleaving
`1
`cos
`ur
`|
`Enzyme
`
`
`
`

`

`Molecular Barcoding & NGS QC
`
`Final gene
`
`SANNAENNENNANNAN
`
`Hybridizegenespecific
`
`Processimutile
`
`separate barcode
`
`Nygenesinparallelwitha
`Yaocprobes
`
`
`
`|eMiseq)
`[scmbybarcode
`
`
`Sequencingvtadlng
`|i
`|i
`
`Mi
`Confirm
`Confirm ——
`
`—
`
`
`
`aeNY
`
`
`
`Poolfrom all genes on water
`
`OC/ES
`
`adaptors
`
`Wsequencing adaptors
`
`’
`
`y
`
`Wsequence
`
`ysequence
`
`y sequence
`
`

`

`Figure 18
`
`
`
`
`
`nkjet assemblyof 10heads
`
`OL/re
`
`plates with 256 nozzles on
`——. 54umcenters
`- 100micronflyheight
`* Each head needsaccess to each
`well thattraverses
`
` \\
`
` \
`
`

`

`Figure 19
`
`ExeEOTAERREANEN
`x
`
`aeaneann
`NEY,
`
`om, Y
`
`haat
`
`25/30
`
`canenr%iiiccgattcihicaieirnnaaa
`teRUAAeALAatetaTtataeatatedteaaEmpgitpetedetienEe
`
`%nee%iraeSess
`Sig,AetrencnneersoaahntEL
`fg,eReeenenmninnee:
`
`“aLCAEAAEODPOOL-
`-:ee,
`
`)Ye
`
`Pron
`t
`
`
`
`
`
`
`

`

`Figure 20
`
`NANINROANONNRHAR,.
`‘x
`
`xx
`
`* \g8g R
`
`oosAa:
`
`
`
`i3ga
`AeHAANRRADRANG
`
`iy 2 ;
`
`*‘$:
`
` enact x
`xxxxxx\xxxxx\*
`
`CtPittaalata
`
`
`gpeininnseinsnecntinaeas
`
`g
`e
`
`
`
`
`HARASS
`
`
`
`
`z g$ LL.g
`
`Of/92
`
`8 ‘g3&S
`
`:gxx
`
`say |
`"
`awai
`
`ony
`ss
`
`Ss 8gS8g88g88s88s88x8g8x3
`
`sx
`Aniaannnnnininn
`
`sna:
`
`xt\‘‘ ‘‘xt
`
`ote
`
`eg
` SootnN
`oui
`
`
`
`LAeg,AN
`
`
`SEE
`
`
`
`
`Storage
`
`
`
`
` *
`
`s (ix
`
`cet
`JOUAANANNRAN
`
`AAA
`
`NIG
`zzgZ!
`
`Hapetapapapatetanaratar
`
`
`
`$ é z g
`
`

`

`Figure 21
`
`aarti
`
`
`AITORAIAIORAIOIMEAIRISRCE
` Henceeeeeben
`
`
`raecicnesiaraecarsintae
`
` gpeiteneeeeereeeey
` fad
`
`
`
`Zz
`
`PoeBasie
`
`FranSerenere,
`
`rn
`
`
`
`
`
`OC/AS
`
`MEASsans
`
` ne
`
` ayes
`MyKERN ES
`
`

`

`Figure 22
`
`
`Ai,
`
`
`
`
`
`43
`
`wow ENG
`
`aa2
`t:paw
`t
`eh8 ‘ ow
`
`x
`Seen
`af
`8.
`x
`
`\amamnnnnnnnnnnnn
`SarniaeNO,
`
` LectEOLEELALLY
`
`
` pecnteatascatddthet SURIMAMIMNS
`
`
`
`_pRASAAAUALGUAAAAAAAAAAAMASERRAERR
`
`Ae Shha He*s eeeeet
`
`a ASN.
`SVK NS
`woe GON
`
`x
`:
`x
`:
`PS
`wf Samay oem

`x Seroroormonnnanonnt
`:
`SSye
`a
`‘
`a
`,
`x s
`s
`x
`:
`3
`:
`7
`:
`"
`‘
`:
`sat
`
`,
`Armor!
`AMALANSEANENATLNSN
`
`Of/8z
`
`
`ay |g48g
`x
`s
`
`
`
`
`care
`ay
`A
`g
`
`
`MAN
`|
`*
`criemanneneonned
`
`sSNARNAANNRUNNARRDRRARCAANUNNRRR
`
`x \<x
`
`3sxi
`
`
`
`x:x:
`
`
`
`‘.
`
`ceieiaeieee
`
`
`:
`Man& NRE|
`
`
`w
`‘
`
`‘
`ulKier a"s
`
`
`pazgg%z¢%zgg&z$zzz
`:
`\caasareateas
`\
`Lass
`. MSHA
`"3
`x
`EAN f
`x

`ay
`En
`ae
`
`}
`UL
`eo.
`Be
`RRR
`i
`
`i
`“OETA
`x
`$
`
`Taviarnmnnnannnnnannnnnnnnnnnt
`
` RARERRRR%
`xxx
`EN SES
`|
`
`{ane|WAY sa
`MAN
`x ‘aiaaniiininatenin
`ANNANAANA‘
`
`
`
` ¥ ernths
`r
`SrosmmnnnanannninenntarcananaaANNNNN
`
`y .xx3xx x>
`
`iperrinnnnntee
`
`~
`
`NHN
`
`xxxxxx
`
`
`
`
`SOMAANNANANSI
`
`x»
`Jara
`
`Jairnec
`Zesepes
`
` JAMMU WW
`
`aeemecrennmcnnennnnnecnnanncannas
`
`g ;kx
`
`
`
`;
`kx
`Mi
`:
`
`i:
`
`xt
`
`Aeitentieaieoneantiia
`
`4
`
`‘
`
`PARMAR peti
`SAARI
`
`;
`
`

`

`Figure 23
`
`1. SOsubstrate with oxide
`
`2, Handle-sice photolithography
`
`3. Handle-sice deepRIE
`
`4. Photoresist strig
`
`5. Device-side photolithography
`
`6. Device-side deepRIE
`
`7, Photoresist & oxide strip
`
`8. Oxide growth
`
`Front-End Processin
`
`
`
` OfC/62
`
`

`

`Figure 24
`
`Back-End Processing
`
`
`. Front-end processing result
`
`_
`
`Inklet photoresist
`
`. Oxygen plasma etch back
`
`_ Passive functionalization
`
`. Photoresist strip
`
`6.
`
`Active functionalization
`
`
`
`
`Oec/O€e
`
`
`
`

Accessing this document will incur an additional charge of $.

After purchase, you can access this document again without charge.

Accept $ Charge

This document could not be displayed.

We could not find this document within its docket. Please go back to the docket page and check the link. If that does not work, go back to the docket and refresh it to pull the newest information.

Your account does not support viewing this document.

You need a Paid Account to view this document. Click here to change your account type.

Your account does not support viewing this document.

Set your membership status to view this document.

With a Docket Alarm membership, you'll get a whole lot more, including:

  • Up-to-date information for this case.
  • Email alerts whenever there is an update.
  • Full text search for other cases.
  • Get email alerts whenever a new case matches your search.

Become a Member

One Moment Please

The filing “” is large (MB) and is being downloaded.

Please refresh this page in a few minutes to see if the filing has been downloaded. The filing will also be emailed to you when the download completes.

Your document is on its way!

If you do not receive the document in five minutes, contact support at support@docketalarm.com.

Sealed Document

We are unable to display this document, it may be under a court ordered seal.

If you have proper credentials to access the file, you may proceed directly to the court's system using your government issued username and password.


Access Government Site

We are redirecting you
to a mobile optimized page.

We are unable to display this document.

HTTP Error 400: Found

Refresh this Document
Go to the Docket