WHATIS CLAIMED IS:
`
`CLAIMS
`
`1.
`
`A method for determining the probability of fragmentation of two genetically linked loci in a
`
`nucleic acid sample comprising:
`a. performing digital PCR (dPCR) on said sample, wherein the dPCR comprises separating
`
`the nucleic acid into separated units;
`
`b. determining a first sum of the numberof units with signal indicating the presence ofa first
`
`locus and the numberof units with signal indicating the presence ofthe first locus and a second locus;
`
`c. determining a second sum of the numberofunits with signal indicating the presence of the
`
`second locus and the numberofunits with signal indicating the presence ofthe first locus and the second
`
`locus;
`
`d. inputing the first and second sumsinto an algorithm to determine the percentage of the two
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`genetically linked loci in the sample that are fragmented.
`
`-17-
`
`WSGRDocket No. 38938-718.101
`
`

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