Abstract
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`System for performing a flow-based assay. The system may comprise a droplet
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`generator to produce an emulsion including droplets in a carrier fluid. The system also
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`may comprise a thermocycler including two or more temperature-controlled zones and
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`also including a channel connected to the droplet generator for receiving the emulsion.
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`The channel may form a single-pass continuous fluid route traversing the temperature-
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`controlled zones multiple times, such that droplets passing through the channel are
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`thermally cycled. The system further may comprise a detection station downstream from
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`the thermocycler and configured to detect a signal from the droplets after such droplets
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`have been thermally cycled by passing through the channel.
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`253
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