Listing of Claims:
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`CLAIMS
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`1.
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`A polynucleotide library, wherein the polynucleotide library comprisesat least
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`20,000 polynucleotides, wherein the at least 20,000 polynucleotides are synthesized based on
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`instructions provided in a computer readable non-transient medium, wherein the at least 20,000
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`polynucleotides encode sequences with an aggregate error rate of less than 1 in 800 bases
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`compared to the preselected sequences received in the instructions provided in the computer
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`readable non-transient medium, wherein each ofthe at least 20,000 polynucleotides comprises a
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`first overlap region which is complementary to a second overlap region of another polynucleotide
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`of the at least 20,000 polynucleotides, such that a plurality of longer nucleic acids are formed
`
`when a subset of the at least 20,000 polynucleotides are assembled, and wherein the subset of the
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`at least 20,000 polynucleotides comprises at least one barcode sequence.
`
`2.
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`The polynucleotide library of claim 1, wherein the at least 20,000 polynucleotides
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`encode sequences with an aggregate error rate of less than | in 1000 bases comparedto the
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`preselected sequences received in the instructions provided in the computer readable non-
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`transient medium.
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`3.
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`The polynucleotide library of claim 1, wherein the at least 20,000 polynucleotides
`
`encode sequences with the aggregate error rate of less than 1 in 800 bases compared to the
`
`preselected sequences received in the instructions provided in the computer readable non-
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`transient medium withouterror correction.
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`4.
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`The polynucleotide library of claim 1, wherein each ofthe at least 20,000
`
`polynucleotides comprises at least one barcode sequence.
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`5.
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`The polynucleotide library of claim 1, wherein each ofthe plurality of longer
`
`nucleic acids comprises at least one barcode sequence.
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`6.
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`The polynucleotide library of claim 1, wherein the at least one barcode sequence
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`comprises up to 50 bases in length.
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`7.
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`The polynucleotide library of claim 1, wherein the first overlap region comprises
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`10 to 100 basesin length.
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`8.
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`The polynucleotide library of claim 1, wherein the first overlap region comprises
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`10 to 50 basesin length.
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`9.
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`The polynucleotide library of claim 1, wherein the first overlap region comprises
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`12 to 25 basesin length.
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`10.—The polynucleotide library of claim 1, wherein thefirst overlap region comprises a
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`GC content of 35% to 65%.
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`WSGRDocketNo. 44854-701.311
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`11.—The polynucleotide library of claim 1, wherein each ofthe at least 20,000
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`polynucleotidesis isolated.
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`12.—The polynucleotide library of claim 1, wherein the polynucleotide library
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`comprisesat least 100,000 polynucleotides.
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`13.
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`The polynucleotide library of claim 1, wherein the polynucleotide library
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`comprisesat least 1,000,000 polynucleotides.
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`14.
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`The polynucleotide library of claim 1, wherein each polynucleotide is at least 25
`
`bases in length.
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`15.
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`The polynucleotide library of claim 1, wherein each polynucleotide is at least 100
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`bases in length.
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`16.—The polynucleotide library of claim 1, wherein each of the longer nucleic acids is
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`at least 0.5 kb in length.
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`17.
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`The polynucleotide library of claim 1, wherein the at least 20,000 polynucleotides
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`are attached to a structure.
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`18.—The polynucleotide library of claim 17, wherein the structureis a solid support.
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`19.
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`A method of nucleic acid assembly, comprising:
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`a) providing preselect sequencesfor at least 20,000 polynucleotides;
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`b) synthesizing the at least 20,000 polynucleotides, wherein each of the at least
`
`20,000 polynucleotides comprisesa first overlap region whichis
`
`complementary to a second overlap region of another polynucleotide ofthe at
`
`least 20,000 polynucleotides, and wherein the at least 20,000 polynucleotides
`
`encode sequences with an aggregate error rate of less than 1 in 800 bases
`
`compared to the preselected sequences; and
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`c) assembling a subset of the at least 20,000 polynucleotides to form a plurality
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`of longer nucleic acids, wherein the subset of the at least 20,000
`
`polynucleotides comprises at least one barcode sequence.
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`20.
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`A method of nucleic acid assembly, comprising:
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`a) providing preselect sequencesfor at least 10,000 nucleic acids;
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`b) synthesizing a plurality of polynucleotides, wherein each polynucleotide of the
`
`plurality of polynucleotides comprisesa first overlap region whichis
`
`complementary to a second overlap region of another polynucleotide of the
`
`plurality of polynucleotides, and wherein the plurality of polynucleotides
`
`encode sequences with an aggregate error rate of less than 1 in 800 bases
`
`compared to the preselected sequences;; and
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`c) assembling a subset of the plurality of polynucleotides to form the at least
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`10086336
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`WSGRDocketNo. 44854-701.311
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`10,000 nucleic acids, wherein the at least 10,000 nucleic acids comprise at
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`least one barcode sequence.
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`10086336
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`-243-
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`WSGRDocketNo. 44854-701.311
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