U.S. Serial No. 15/729,564
`Response to Non-Final Office Action Filed November 26, 2019
`
`Attorney Docket No. 44854-701309
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`AMENDMENTS TO THE CLAIMS
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`This listing of claims replaces all prior versions and listing of claims in the above-
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`referenced patent application. The following amendments do not constitute an admission
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`regarding the patentability of the amended subject matter and should not be so construed.
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`Amendments to the claims were made for purposes of more clearly stating the claimed subject
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`matter and do not add new matter or alter the scope of the claims. Deletions are denoted by
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`[strikethreugh] and additions are denoted by underline.
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`Listing of the Claims
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`1.
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`(Currently Amended) A polynucleotide cDNA library, wherein the
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`polynucleotide cDNA library comprises at least 20,000 polynucleotides, wherein each of the at
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`least 20,000 polynucleotides is at least [[75]] m bases in length and synthesized based on
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`instructions provided in a computer readable non-transient medium for synthesis of preselected
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`cDNA sequences, wherein each of the at least 20,000 polynucleotides comprises a first overlap
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`region which is complementary to a second overlap region of another polynucleotide of the at
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`least 20,000 polynucleotides, such that a plurality of genes are formed when a subset of the at
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`least 20,000 polynucleotides are assembled, wherein the first overlap region comprises at least
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`10 bases in length, and wherein the at least 20,000 polynucleotides encode sequences with an
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`aggregate error rate of less than 1 in [[800]] w bases compared to the preselected cDNA
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`sequences received in the instructions provided in the computer readable non-transient medium.
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`2.
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`3.
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`4.
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`Cancelled.
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`Cancelled.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein the
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`first overlap region comprises a GC content of 35% to 65%.
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`5.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein the
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`first overlap region comprises 10 to 100 bases in length.
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`6.
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`7.
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`Cancelled.
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`(Original) The polynucleotide cDNA library of claim 1, wherein each of the at
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`least 20,000 polynucleotides is isolated.
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`8.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the
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`polynucleotide cDNA library comprises at least 60,000 polynucleotides.
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`-2-
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`

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`U.S. Serial No. 15/729,564
`Response to Non-Final Office Action Filed November 26, 2019
`
`Attorney Docket No. 44854-701309
`
`9.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the
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`polynucleotide cDNA library comprises at least 100,000 polynucleotides.
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`10.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the
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`polynucleotide cDNA library comprises at least 1,000,000 polynucleotides.
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`11.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 1000 genes are formed when a subset of the at least 20,000 polynucleotides are assembled.
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`12.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 10,000 genes are formed when a subset of the at least 20,000 polynucleotides are
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`assembled.
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`13.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 100,000 genes are formed when a subset of the at least 20,000 polynucleotides are
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`assembled.
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`14.
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`(Currently Amended) The polynucleotide cDNA library of claim 1, wherein
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`each polynucleotide is 8046600 100 to 300 bases in length.
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`15.
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`(Currently Amended) The polynucleotide cDNA library of claim 1, wherein
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`each polynucleotide is [[80]] m to 200 bases in length.
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`16.
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`17.
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`18.
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`Cancelled.
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`Cancelled.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein
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`each of the genes is at least 0.5 kb in length.
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`19.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein
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`each of the genes is at least 3 kb in length.
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`20.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein the
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`at least 20,000 polynucleotides are attached to a structure.
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`21.
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`(Original) The polynucleotide cDNA library of claim 20, wherein the structure is
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`a solid support.
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`22.
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`(Currently Amended) The polynucleotide cDNA library of claim 1, wherein the
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`at least 20,000 polynucleotides encode sequences with the aggregate error rate of less than 1 in
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`[[800]] w bases compared to the preselected cDNA sequences received in the instructions
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`provided in the computer readable non-transient medium without error correction.
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`23.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein the
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`first overlap region comprises 10 to 50 bases in length.
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`-3-
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`

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`U.S. Serial No. 15/729,564
`Response to Non-Final Office Action Filed November 26, 2019
`
`Attorney Docket No. 44854-701309
`
`24.
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`(Withdrawn / Currently Amended) A method for polynucleotide synthesis,
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`comprising:
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`a) providing instructions in a computer readable non-transient medium for
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`synthesis of preselected cDNA sequences; and
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`b) synthesizing at least 20,000 polynucleotides encoding for the preselected
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`cDNA sequences, wherein each of the at least 20,000 polynucleotides is at least [[75]]
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`m bases in length, wherein each of the at least 20,000 polynucleotides comprises a first
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`overlap region which is complementary to a second overlap region of another
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`polynucleotide of the at least 20,000 polynucleotides such that a plurality of genes are
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`formed when a subset of the at least 20,000 polynucleotides are assembled, wherein the
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`first overlap region comprises at least 10 bases in length, and wherein the at least 20,000
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`polynucleotides encode sequences with an aggregate error rate of less than 1 in [[800]]
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`w bases compared to the preselected cDNA sequences received in the instructions
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`provided in the computer readable non-transient medium.
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`25.
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`26.
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`Cancelled.
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`(Withdrawn) The method of claim 24, wherein at least 100,000 polynucleotides
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`are synthesized.
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`27.
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`(Withdrawn) The method of claim 24, wherein at least 750 genes are formed
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`when a subset of the at least 20,000 polynucleotides are assembled.
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`28.
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`(Withdrawn) The method of claim 24, wherein at least 10,000 genes are formed
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`when a subset of the at least 20,000 polynucleotides are assembled.
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`29.
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`(Withdrawn) The method of claim 24, wherein the first overlap region comprises
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`a GC content of 35% to 65%.
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`30.
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`(Withdrawn) The method of claim 24, wherein the first overlap region comprises
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`10 to 100 bases in length.
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`3 l.
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`(Withdrawn/Currently Amended) The method of claim 24, wherein each
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`polynucleotide is 8046600 100 to 300 bases in length.
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`32.
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`(Withdrawn/Currently Amended) The method of claim 24, wherein each
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`polynucleotide is [[80]] m to 200 bases in length.
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`33.
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`34.
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`kb in length.
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`Cancelled.
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`(Withdrawn) The method of claim 24, wherein each of the genes is at least 0.5
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`

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`U.S. Serial No. 15/729,564
`Response to Non-Final Office Action Filed November 26, 2019
`
`Attorney Docket No. 44854-701309
`
`35.
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`(Withdrawn) The method of claim 24, wherein each of the genes is at least 3 kb
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`inlengfli
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`36.
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`(Withdrawn) The method of claim 24, wherein the at least 20,000
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`polynucleotides are synthesized on a structure.
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`37.
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`38
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`(Withdrawn) The method of claim 36, wherein the structure is a solid support.
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`(“Mhmawn/CmTHMyAnwmkdymemammofdmm24“madn$%w%
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`W the at least 20,000 polynucleotides have—ne—er-rers encode seguences with the aggregate
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`error rate of less than 1 in 1000 bases compared to the preselected cDNA sequences received in
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`the instructions provided in the computer readable non-transient medium without error
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`correction.
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`39.
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`(Withdrawn) The method of claim 24, further comprising hybridizing each
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`polynucleotide said subset to at least another polynucleotide in said to form the plurality of
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`genes.
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