`
`1.
`
`A method of analysis, the method comprising:
`
`selecting a device having a port connected to a chamber;
`
`placing a sample-containing fluid into the port;
`
`creating a pressure differential that drives the sample-containing fluid from the
`
`port to the chamber and separates the sample-containing fluid into droplets;
`
`forming a two-dimensional monolayer of the droplets in the chamber; and
`
`imaging at least a portion of the monolayer.
`
`2.
`
`The method of claim 1, wherein the step of placing includes a step of
`
`placing the sample-containing fluid as a continuous phase into a well of the device.
`
`3.
`
`The method of claim 1, wherein the droplets are aqueous droplets that are
`
`separated from one another by an immiscible carrier liquid.
`
`4.
`
`The method of claim 1, wherein the step of forming includes a step of
`
`collecting the droplets in a chamber having a height corresponding to a diameter of the
`
`droplets.
`
`5.
`
`The method of claim 1, wherein the step of creating a pressure differential
`
`includes a step of applying positive gas pressure or negative gas pressure to a port of
`
`the device.
`
`6.
`
`The method of claim 1, further comprising a step of thermally cycling the
`
`monolayer to promote nucleic acid amplification in a subset of droplets of
`
`the
`
`monolayer.
`
`7.
`
`The method of claim 1, wherein the step of imaging includes a step of
`
`detecting fluorescence from the monolayer.
`
`256
`
`
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