U.S. Serial No. 15/603,013
`Response to Non-Final Office Action dated June 26, 2019
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`Attorney Docket No. 44854-701402
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`AMENDMENTS TO THE CLAIMS
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`This listing of claims will replace all prior versions, and listings, of claims in this
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`application. The following amendments do not constitute an admission regarding the
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`patentability of the amended subject matter and should not be so construed. Applicant reserves
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`the right to pursue the subject matter of the canceled claims in this or any other appropriate
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`patent application.
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`Listing of Claims:
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`1.
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`(Currently Amended) A nucleic acid cDNA library comprising nucleic acids
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`synthesized based on instructions provided in a computer readable non-transient medium for
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`synthesis of preselected cDNA sequences collectively encoding for at least 1000 genes, wherein
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`each of the at least 1000 genes is a different genomic M sequence that comprises at least one
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`intron, wherein the nucleic acids encode cDNA sequences with an aggregate error rate of less
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`than 1 in 800 bases compared to the preselected cDNA sequences received in the instructions
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`provided in the computer readable non-transient medium, wherein the nucleic acids comprise
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`sub sets of nucleic acids, and wherein each of the subsets of nucleic acids comprises gfi
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`fragment nucleic acids th_at:
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`(i) vafiLi-ng—voLi-Ehin—a—Eange—efllengths have a plurality of lengths,
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`(ii) collectively spanning gm at least 0.5 kb in length, and
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`(iii) collectively encoding encode cDNA sequence for one of the at least 1000 genes,
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`wherein at least one of the gene fragment nucleic acids in each of the subsets of nucleic
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`acids encodes cDNA sequence lacking the at least one intron.
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`2.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`nucleic acids encode cDNA sequences with an aggregate error rate of less than 1 in 1000 bases
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`compared to the preselected cDNA sequences received in the instructions provided in the
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`computer readable non-transient medium.
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`3.
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`(Original) The nucleic acid cDNA library of claim 1, wherein each of the nucleic
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`acids is isolated.
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`4.
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`(Original) The nucleic acid cDNA library of claim 1, wherein each of the nucleic
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`acids is purified.
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`5.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`-2-
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`
`
`U.S. Serial No. 15/603,013
`Response to Non-Final Office Action dated June 26, 2019
`
`Attorney Docket No. 44854-701402
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`nucleic acids collectively encode cDNA sequences for at least 10,000 genes.
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`6.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`nucleic acids collectively encode cDNA sequences for at least 100,000 genes.
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`7.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`nucleic acids collectively encode cDNA sequences for at least 1,000,000 genes.
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`8.
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`9.
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`Cancelled.
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`(Currently Amended) The nucleic acid cDNA library of claim 1, wherein each of
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`the subsets of nucleic acids comprises gene fragment nucleic acids collectively spanning at least
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`1 kb in length.
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`10.
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`(Currently Amended) The nucleic acid cDNA library of claim 1, wherein each of
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`the subsets of nucleic acids comprises gene fragment nucleic acids collectively spanning at least
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`3 kb in length.
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`11.
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`12.
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`Cancelled.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`nucleic acids are attached to a structure.
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`13.
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`(Original) The nucleic acid cDNA library of claim 12, wherein the structure is a
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`solid support.
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`14.
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`17.
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`-16. Cancelled.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`nucleic acids are synthesized by a process comprising chemical synthesis on a solid support.
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`18.
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`(Previously Presented) The nucleic acid cDNA library of claim 17, wherein the
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`solid support comprises a plurality of molecules bound to the solid support at loci, and wherein
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`the plurality of molecules comprise a reactive group capable of coupling to a nucleoside.
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`19.
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`(Previously Presented) The nucleic acid cDNA library of claim 17, wherein the
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`solid support is a plate.
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`20.
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`(Previously Presented) The nucleic acid cDNA library of claim 17, wherein the
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`solid support comprises silicon, polypropylene, or polydimethylsiloxane (PDMS).
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`21.
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`22.
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`Cancelled.
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`(Currently Amended) The nucleic acid cDNA library of claim [[24]] 1, wherein
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` - - each of the nucleic acids
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`comprises at least one barcode sequence for identification of each of said nucleic acids.
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`23.
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`(Currently Amended) The nucleic acid cDNA library of claim [[24]] 2, wherein
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`-3-
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`U.S. Serial No. 15/603,013
`Response to Non-Final Office Action dated June 26, 2019
`
`Attorney Docket No. 44854-701402
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`each of the nucleic acids comprises more than one barcode sequence.
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`24.
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`(Currently Amended) The nucleic acid cDNA library of claim [[1]] Q, wherein
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`
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`sand—nueleieaeids wherein the at least one barcode sequence comprises up to 50 bases in length.
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`25.
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`(Currently Amended) The nucleic acid cDNA library of claim 1, wherein each of
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`the nucleic acids is 80 to 200 bases pair—s in length.
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`26.
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`(Currently Amended) The nucleic acid cDNA library of claim 1, wherein the
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`plurality of lengths is nueleieaeids—vaiynig—withi-n—a—Pfiige-eflleng-ths—afe-ef at least 10 different
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`lengths.
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`27.
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`(Previously Presented) The nucleic acid cDNA library of claim 1, wherein the
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`nucleic acids are double stranded.
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`28.
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`(Currently Amended) A nucleic acid cDNA library comprising nucleic acids
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`synthesized based on instructions provided in a computer readable non-transient medium for
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`synthesis of preselected cDNA sequences collectively encoding for at least 1000 genes, wherein
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`each of the at least 1000 genes is a different genomic DNA sequence that comprises at least one
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`m, wherein the nucleic acids comprise subsets of nucleic acids, and wherein each of the
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`subsets of nucleic acids comprises gene fragment nucleic acids M:
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`(i) va-nyi-ng—wrthi-n—a—nange-eflleng-‘ehs have a plurality of lengths;
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`(ii) collectively spanning gm at least 0.5 kb in length; and
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`(iii) collectively eneedi—ng encode cDNA sequence for one of the at least 1000 genes;
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`wherein at least one of the gene fragment nucleic acids in each of the subsets of nucleic
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`acids encodes cDNA sequence lacking the at least one intron aherei—n—eneh—eflthe—at—lenst
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