U.S. Serial No. 15/602,991
`Response to Final Office Action Filed December 19, 2018
`
`Attorney Docket No. 44854-701308
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`AMENDMENTS TO THE CLAIMS
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`This listing of claims replaces all prior versions and listing of claims in the above-
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`referenced patent application. The following amendments do not constitute an admission
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`regarding the patentability of the amended subject matter and should not be so construed.
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`Amendments to the claims were made for purposes of more clearly stating the claimed subject
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`matter and do not add new matter or alter the scope of the claims. Deletions are denoted by
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`[strikethreugh] and additions are denoted by underline.
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`Listing of the Claims
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`1.
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`(Currently Amended) A polynucleotide cDNA library, wherein the
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`polynucleotide cDNA library comprises at least 20,000 polynucleotides, wherein each of the at
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`least 20,000 polynucleotides [[are]] is at least 75 bases in length and synthesized based on
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`instructions provided in a computer readable non-transient medium for synthesis of preselected
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`cDNA sequences, wherein at least 80% of the at least 20,000 polynucleotides have no errors
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`compared to the preselected cDNA sequences received in the instructions provided in the
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`computer readable non-transient medium, wherein each of the at least 20,000 polynucleotides
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`comprises a first overlap region which is complementary to a second overlap region of another
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`polynucleotide of the at least 20,000 polynucleotides, such that a plurality of genes are formed
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`when a subset of the at least 20,000 polynucleotides are assembled, and wherein the first overlap
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`region comprises at least 10 bases in length.
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`2.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 89% of the at least 20,000 polynucleotides have no errors compared to the preselected
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`cDNA sequences received in the instructions provided in the computer readable non-transient
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`medium.
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`3.
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`(Previously presented) The polynucleotide cDNA library of claim 1, wherein
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`each of the at least 20,000 polynucleotides is isolated.
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`4.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the
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`polynucleotide cDNA library comprises at least 50,000 polynucleotides.
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`5.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the
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`polynucleotide cDNA library comprises at least 100,000 polynucleotides.
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`6.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the
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`-2-
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`
`U.S. Serial No. 15/602,991
`Response to Final Office Action Filed December 19, 2018
`
`Attorney Docket No. 44854-701308
`
`polynucleotide cDNA library comprises at least 1,000,000 polynucleotides.
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`7.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 750 genes are formed when a subset of the at least 20,000 polynucleotides are assembled.
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`8.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 2000 genes are formed when a subset of the at least 20,000 polynucleotides are assembled.
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`9.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 10,000 genes are formed when a subset of the at least 20,000 polynucleotides are
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`assembled.
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`10.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 100,000 genes are formed when a subset of the at least 20,000 polynucleotides are
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`assembled.
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`11.
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`12.
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`Cancelled.
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`(Previously presented) The polynucleotide cDNA library of claim 1, wherein the
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`first overlap region comprises a GC content of 35% to 65%.
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`13.
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`(Previously presented) The polynucleotide cDNA library of claim 1, wherein the
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`first overlap region comprises 10 to 100 bases in length.
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`14.
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`(Currently Amended) The polynucleotide cDNA library of claim 1, wherein
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`each polynucleotide is [[30]]& to 500 bases in length.
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`15.
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`(Currently Amended) The polynucleotide cDNA library of claim 1, wherein
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`each polynucleotide is at—least—L’é 80 to 200 bases in length.
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`16.
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`(Original) The polynucleotide cDNA library of claim 1, wherein each
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`polynucleotide is at least 100 bases in length.
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`17.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein
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`each of the genes is at least 0.5 kb in length.
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`18.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein
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`each of the genes is at least 3 kb in length.
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`19.
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`(Original) The polynucleotide cDNA library of claim 1, wherein the at least
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`20,000 polynucleotides are attached to a structure.
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`20.
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`(Original) The polynucleotide cDNA library of claim 19, wherein the structure is
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`a solid support.
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`21.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein at
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`least 80% of the at least 20,000 polynucleotides have no errors compared to the preselected
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`-3-
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`
`U.S. Serial No. 15/602,991
`Response to Final Office Action Filed December 19, 2018
`
`Attorney Docket No. 44854-701308
`
`cDNA sequences received in the instructions provided in the computer readable non-transient
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`medium without error correction.
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`22.
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`(Previously Presented) The polynucleotide cDNA library of claim 1, wherein the
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`first overlap region comprises 10 to 50 bases in length.
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`23.
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`(New) A method for polynucleotide synthesis, comprising:
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`a) providing instructions in a computer readable non-transient medium for
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`synthesis of preselected cDNA sequences, and
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`b) synthesizing at least 20,000 polynucleotides encoding for the preselected
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`cDNA sequences, wherein each of the at least 20,000 polynucleotides is at least 75 bases
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`in length, wherein each of the at least 20,000 polynucleotides comprises a first overlap
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`region which is complementary to a second overlap region of another polynucleotide of
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`the at least 20,000 polynucleotides such that a plurality of genes are formed when a
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`subset of the at least 20,000 polynucleotides are assembled, wherein the first overlap
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`region comprises at least 10 bases in length, and wherein at least 80% of the at least
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`20,000 polynucleotides have no errors compared to the preselected cDNA sequences in
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`the instructions provided in the computer readable non-transient medium.
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`24.
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`(New) The method of claim 23, wherein at least 89% of the at least 20,000
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`polynucleotides have no errors compared to the preselected cDNA sequences received in the
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`instructions provided in the computer readable non-transient medium.
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`25.
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`(New) The method of claim 23, wherein at least 100,000 polynucleotides are
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`synthesized.
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`26.
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`(New) The method of claim 23, wherein at least 750 genes are formed when a
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`subset of the at least 20,000 polynucleotides are assembled.
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`27.
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`(New) The method of claim 23, wherein at least 10,000 genes are formed when a
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`subset of the at least 20,000 polynucleotides are assembled.
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`28.
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`(New) The method of claim 23, wherein the first overlap region comprises a GC
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`content of35% to 65%.
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`29.
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`(New) The method of claim 23, wherein the first overlap region comprises 10 to
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`100 bases in length.
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`30.
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`(New) The method of claim 23, wherein each polynucleotide is 80 to 500 bases in
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`length.
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`3 1.
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`(New) The method of claim 23, wherein each polynucleotide is 80 to 200 bases in
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`-4-
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`U.S. Serial No. 15/602,991
`Response to Final Office Action Filed December 19, 2018
`
`Attorney Docket No. 44854-701308
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`length.
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`32.
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`in length.
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`length.
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`length.
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`33.
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`34.
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`35.
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`(New) The method of claim 23, wherein each polynucleotide is at least 100 bases
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`(New) The method of claim 23, wherein each of the genes is at least 0.5 kb in
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`(New) The method of claim 23, wherein each of the genes is at least 3 kb in
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`(New) The method of claim 23, wherein the at least 20,000 polynucleotides are
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`synthesized on a structure.
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`36.
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`37.
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`(New) The method of claim 35, wherein the structure is a solid support.
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`(New) The method of claim 23, wherein at least 80% of the at least 20,000
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`polynucleotides have no errors compared to the preselected cDNA sequences received in the
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`instructions provided in the computer readable non-transient medium without error correction.
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`38.
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`(New) The method of claim 23, further comprising hybridizing each
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`polynucleotide said subset to at least another polynucleotide in said to form the plurality of
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`genes.
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