U.S. Serial No.: 15/187,714
`Response to Non-Final Office Action filed April 4, 2019
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`Attorney Docket No. 44854-701304
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`AMENDMENTS TO THE CLAIMS
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`This listing of claims will replace all prior versions, and listings, of claims in this
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`application. The following amendments do not constitute an admission regarding the
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`patentability of the amended subject matter and should not be so construed. Applicants reserve
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`the right to pursue the subject matter of the canceled claims in this or any other appropriate
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`patent application.
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`Claims:
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`1-22. (Cancelled)
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`23. (Currently Amended) A method for computer-assisted nucleic acid synthesis, comprising:
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`receiving instructions in a computer readable non-transient medium for synthesis
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`of cDNA sequences encoding for at least 750 genes,
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`processing the instructions in a computer and transmitting synthesis instructions
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`to a material deposition device, wherein the synthesis instructions provide for synthesis
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`of a plurality of polynucleotides that collectively encode for the cDNA sequences,
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`releasing synthesis reagents from the material deposition device to synthesize the
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`plurality of polynucleotides, wherein the plurality of polynucleotides encode sequences
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`with an aggregate error rate of less than 1 in [[800]] w bases without correcting errors
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`compared to the cDNA sequences received in the instructions in the computer readable
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`non-transient medium, and
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`assembling a plurality of nucleic acids from a subset of the plurality of
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`polynucleotides, wherein the plurality of nucleic acids comprise sequences encoded by
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`the cDNA sequences encoding for at least 750 genes.
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`24.
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`25.
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`(Cancelled)
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`(Previously Presented) The method of claim 23, wherein each nucleic acid of the
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`plurality of nucleic acids is isolated.
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`26.
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`(Previously Presented) The method of claim 23, wherein each nucleic acid of the
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`plurality of nucleic acids is purified.
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`27.
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`(Previously Presented) The method of claim 23, wherein each of the plurality of
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`polynucleotides extends from a surface.
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`28.
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`(Previously Presented) The method of claim 23, further comprising treating the
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`plurality of nucleic acids with an error correction enzyme.
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`-2-
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`U.S. Serial No.: 15/187,714
`Response to Non-Final Office Action filed April 4, 2019
`
`Attorney Docket No. 44854-701304
`
`29.
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`(Previously Presented) The method of claim 23, wherein each nucleic acid of the
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`plurality of nucleic acids is at least 0.5 kb long.
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`30.
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`(Previously Presented) The method of claim 23, wherein each nucleic acid of the
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`plurality of nucleic acids is at least 3 kb long.
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`3 1.
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`(Previously Presented) The method of claim 23, wherein the plurality of
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`polynucleotides comprises at least 60,000 polynucleotides.
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`32.
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`(Previously Presented) The method of claim 23, wherein the plurality of
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`polynucleotides comprises at least 100,000 polynucleotides.
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`33.
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`(Currently Amended) The method of claim 23, wherein the plurality of
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`polynucleotides comprises at least 609,000 polynucleotides.
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`34.
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`(Currently Amended) The method of claim 23, wherein the [[the]] plurality of
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`polynucleotides collectively encode and correspond to cDNA sequences for at least 1000 genes.
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`35.
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`(Previously Presented) The method of claim 23, wherein each polynucleotide is
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`30 to 500 bases in length.
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`36.
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`(Previously Presented) The method of claim 23, wherein each polynucleotide is at
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`least 25 bases in length.
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`37.
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`(New) The method of claim 23, wherein each polynucleotide is 80 to 200 bases in
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`length.
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`38.
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`(New) The method of claim 23, wherein the aggregate error rate is determined by
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`sequencing following amplification of the plurality of polynucleotides.
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`39.
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`(New) The method of claim 38, wherein the sequencing is performed using
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`Sanger sequencing method.
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`40.
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`(New) The method of claim 38, wherein the amplification is performed using a
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`DNA polymerase.
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