`
`Sequence Listing was accepted.
`
`
`
`
`If you need help call the Patent ?1ectronic Qusiness Center at
`
`217—9197 (toll free).
`
`(866)
`
`Reviewer: Anjum, Durreshwar
`
`sec=38; ms=46l;
`[year=2018; month=4; day=30; hr=l2; min=ll;
`Timestamp:
`
`
`]
`
`

`

`Validated By CRFValidator v 1,0,5
`
`Application No:
`
`15121623
`
`Version No:
`
`10
`
`Input Set:
`
`Output Set:
`
`Started:
`
`2018—04—27 14:06:38.859
`
`Finished:
`
`2018—04—27 14:06:40.311
`
`0 min(s)
`
`l sec(s)
`
`452 ms
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
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`
`<7n3>
`?Q
`
`fld
`
`fld
`
`fld
`
`fld
`
`0 hr(s)
`175
`
`0 1
`
`75
`
`175
`
`flown
`
`50L
`
`flown
`
`50L
`
`Elapsed:
`
`Total Warnings:
`
`Total Errors:
`
`No. of SquDs Defined:
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`Actual SquD Count:
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`Error Description
`II
`
`
`II
`
`Ar
`
`II
`
`II
`
`II
`
`Ar
`
`Ar
`
`Ar
`
`II
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`Error code
`
`W
`
`W
`
`W
`
`W
`
`2u3
`
`2u3
`
`2H3
`
`2u3
`
`W
`
`W
`
`W
`
`W
`
`W
`
`W
`
`2u3
`
`2H3
`
`2u3
`
`2u3
`
`2H3
`
`2u3
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`II
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`II
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`II
`
`II
`
`II
`
`Ar
`
`Ar
`
`Ar
`
`Ar
`
`Ar
`
`Ar
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`fld
`
`fld
`
`fld
`
`fld
`
`fld
`
`fld
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`
`2u3
`
`2H3
`
`2u3
`
`II
`
`II
`
`II
`
`Ar
`
`Ar
`
`Ar
`
`W
`
`W
`
`W
`
`W
`
`
`
`
`
`
`
`
`
`or on
`
`or on
`
`
`
`
`
`
`
`flown
`
`flown
`
`flown
`
`
`
`
`
`50L
`
`50L
`
`50L
`
`
`
`fld
`
`fld
`
`fld
`
`II
`
`'C'a
`
`
`
`
`
`
`
`
`
`II
`
`'C'a
`II
`
`
`
`
`
`
`
`2u3
`
`II
`
`
`II
`
`Ar
`
`flown
`
`50L
`
`fld
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`
`
`
`
`
`
`
`
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`W
`
`W
`
`W
`
`W
`
`W
`
`W
`
`2u3
`
`2H3
`
`2u3
`
`2u3
`
`2H3
`
`2u3
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
`'C'a
`
`II
`
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`
`II
`
`'C'a
`
`II
`
`II
`
`II
`
`II
`
`II
`
`II
`
`Ar
`
`Ar
`
`Ar
`
`Ar
`
`Ar
`
`Ar
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`or on
`
`flown
`
`50L
`
`fld
`
`fld
`
`fld
`
`fld
`
`fld
`
`fld
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`
`<7n3>
`?Q
`
`

`

`Input Set:
`
`Output Set:
`
`Started:
`
`2018—04—27 14:06:38.859
`
`Finished:
`
`2018—04—27 14:06:40.311
`
`Elapsed:
`
`0 hr(s)
`
`0 min(s)
`
`1 sec(s) 452 ms
`
`Total Warnings:
`
`Total Errors:
`
`No. of SquDs Defined:
`
`Actual SquD Count:
`
`175
`
`0
`
`175
`
`175
`
`Error code
`
`Error Description
`
`This error has occured more than 20 times, will not be displayed
`
`

`

`
`
` O]
`
`*QUPNC‘ PISLING
`
`<110>
`
`MERUS N.V.
`
`G‘UIJ‘N, C‘CILIA A.W
`DE KRJIF, CORN L‘J AIS A.
`
` "{ROSBY, MARK
`
`
`
`
`
` .OGL‘ B‘RG, MAR<
`A
`
`BAKK
`R, AL‘XAND‘R B.H
`
`
`
`Antibody tqat binds Erbb—Z and
`
`
`Erbb—3
`
`/\ "30>
`
`<140>
`/
`("41>
`
`<150>
`/
`("51>
`
`
`
`/\ "50>
`<"51>
`
`/\ "50>
`<"51>
`
`A "60>
`
`<170>
`
`4096.0100002/DAS/PAC/E—H
`
`US 15/121,623
`2016—08—25
`
`PCT/NL15/050125
`2015—02—27
`
`3P 14157360.0
`2014—02—28
`
` 3P 14167066.1
`
`2014—05—05
`
`175
`
`PatentIn version 3.5
`
`1 3
`
`6
`DNA
`
`Artificial Sequence
`
`primer
`
`<210>
`<21l>
`<212>
`<213>
`
`<220>
`<223>
`
`<400>
`
`1
`
`aagctggcta gcaccatgga gctggcggcc ttgtgc
`
`2 3
`
`2
`DNA
`
`Artificial Sequence
`
`primer
`
`<210>
`<2ll>
`<212>
`<213>
`
`<220>
`<223>
`
`<400>
`
`2
`
`aataattcta gactggcacg tccagaccca gg
`
`3 3
`
`6
`DNA
`
`<210>
`<2ll>
`<212>
`
`

`

`<213> Artificial Sequence
`
`<220>
`
`<223>
`
`primer
`
`<400>
`
`3
`
`aagctggcta gcaccatgga gctgchgcc tggtac
`
`<210>
`<211>
`<212>
`
`4
`32
`DNA
`
`<213> Artificial Sequence
`
`<220>
`
`<223>
`
`primer
`
`<400>
`
`4
`
`aataattcta gactggcacg tccagaccca gg
`
`<210>
`<211>
`<212>
`
`5
`36
`DNA
`
`<213> Artificial Sequence
`
`<220>
`
`<223>
`
`primer
`
`<400>
`
`5
`
`aagctggcta gcaccatgag ggcgaacggc gctctg
`
`<210>
`<211>
`<212>
`
`6
`33
`DNA
`
`<213> Artificial Sequence
`
`<220>
`
`<223>
`
`primer
`
`<400>
`
`6
`
`aataattcta gattacgttc tctgggcatt agc
`
`7
`<210>
`<le> 370
`<212>
`DNA
`
`<213> Artificial Sequence
`
`<220>
`
`<223> MF2926: heavy chain variable region
`antibody
`
`sequence of an erbB—2 binding
`
`<220>
`<221>
`
`CDS
`
`<222>
`
`(20)..(370)
`
`32
`
`36
`
`33
`
`

`

`<400>
`
`7
`
`ggcccagccg gccatggcc cag gtc cag ctg cag cag tct gga cct gag ctg
`Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu
`1
`5
`10
`
`gtg aaa cct ggg gct tca gtg atg att tcc tgc aag gct tct ggt tac
`Val Lys Pro Gly Ala Ser Val Met Ile Ser Cys Lys Ala Ser Gly Tyr
`15
`20
`25
`
`tca ttc act ggc tac cac atg aac tgg gtg aag caa agt cct gaa aag
`Ser Phe Thr Gly Tyr His Met Asn Trp Val Lys Gln Ser Pro Glu Lys
`30
`35
`40
`
`agc ctt gag tgg att gga gac ata aat cct agc att ggt acg act gcc
`Ser Leu Glu Trp Ile Gly Asp Ile Asn Pro Ser Ile Gly Thr Thr Ala
`45
`50
`55
`
`cac aac cag att ttc agg gcc aag gcc aca atg act gtt gac aaa tcc
`His Asn Gln Ile Phe Arg Ala Lys Ala Thr Met Thr Val Asp Lys Ser
`60
`65
`70
`75
`
`tcc aac aca gcc tac atg cag ctc aag agc ctg aca tct gaa gac tct
`Ser Asn Thr Ala Tyr Met Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser
`80
`85
`90
`
`gga gtc ttt tac tgt gtt aga aga ggg gac tgg tcc ttc gat gtc tgg
`Gly Val Phe Tyr Cys Val Arg Arg Gly Asp Trp Ser Phe Asp Val Trp
`95
`100
`105
`
`ggc aca ggg acc acg gtc acc gtc tcc agt
`Gly Thr Gly Thr Thr Val Thr Val Ser Ser
`110
`115
`
`52
`
`100
`
`148
`
`196
`
`244
`
`292
`
`340
`
`370
`
`<210>
`<211>
`<212>
`
`8
`117
`PRT
`
`<213> Artificial Sequence
`
`<220>
`
`<223> Synthetic Construct
`
`<400>
`
`8
`
`Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala
`
`Ser Val Met Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr
`20
`25
`30
`
`His Met Asn Trp Val Lys Gln Ser Pro Glu Lys Ser Leu Glu Trp Ile
`35
`40
`45
`
`Gly Asp Ile Asn Pro Ser Ile Gly Thr Thr Ala His Asn Gln Ile Phe
`
`

`

`50
`
`55
`
`6O
`
`Arg Ala Lys Ala Thr Met Thr Val Asp Lys Ser Ser Asn Thr Ala Tyr
`65
`70
`75
`80
`
`Met Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Gly Val Phe Tyr Cys
`85
`9O
`95
`
`Val Arg Arg Gly Asp Trp Ser Phe Asp Val Trp Gly Thr Gly Thr Thr
`100
`105
`110
`
`Val Thr Val Ser Ser
`115
`
`<210>
`<211>
`<212>
`
`9
`16
`PRT
`
`<213> Artificial Sequence
`
`<220>
`<223> MF2926 CDRl
`
`<400>
`
`9
`
`Gly Tyr His Met Asn Trp Val Lys Gln Ser Pro Glu Lys Ser Leu Glu
`1
`5
`10
`15
`
`<210>
`<211>
`<212>
`
`10
`6
`PRT
`
`<213> Artificial Sequence
`
`<220>
`<223> MF2926 CDR2
`
`<400>
`
`10
`
`Asn Gln Ile Phe Arg Ala
`1
`5
`
`<210>
`<211>
`<212>
`
`11
`8
`PRT
`
`<213> Artificial Sequence
`
`<220>
`<223> MF2926 CDR3
`
`<400>
`
`ll
`
`

`

`Arg Gly Asp Trp Ser Phe Asp Val
`1
`5
`
`<210>
`<21l>
`<212>
`<213>
`
`<220>
`<223>
`
`<220>
`<221>
`<222>
`
`12
`379
`DNA
`
`Artificial Sequence
`
`MF2930:
`
`antibody
`
`heavy chain variable region sequence of an erbB—2 binding
`
`CDS
`
`(20) .. (379)
`
`<400>
`
`12
`
`ggcccagccg gccatggcc gag gtc cag ctg cag cag tct ggg gct gaa ctg
`
`52
`
`Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu
`1
`5
`10
`
`tac
`
`100
`
`ggc
`
`Gly
`
`148
`
`196
`
`244
`
`gtg
`Val
`
`aag
`
`Lys
`
`cct
`Pro
`
`acc
`
`Thr
`
`ttc
`Phe
`
`act
`Thr
`30
`
`gga
`
`Gly
`15
`
`tcc
`Ser
`
`gcc
`Ala
`
`tca
`(1
`oer
`
`gtg
`Val
`
`atg
`Met
`
`tcc
`(1
`oer
`
`tgt
`Cys
`
`aag
`
`Lys
`
`gtt
`Val
`
`atg
`Met
`20
`
`tat
`
`cct
`Pro
`
`ata
`Ile
`
`gcg
`Ala
`35
`
`tgg
`Trp
`
`atg
`Met
`
`aag
`
`Lys
`
`cag
`Gln
`
`gtt
`Val
`40
`
`tct
`(1
`oer
`
`25
`
`cat
`His
`
`gga
`
`aag
`
`Gly
`
`Lys
`
`cta
`Leu
`45
`
`agc
`Ser
`
`tac
`
`gag
`Glu
`
`tgg
`Trp
`
`att
`
`Ile
`
`gga
`
`Gly
`
`aat
`Asn
`50
`
`ttt
`
`cat
`
`Phe
`
`His
`
`cct
`Pro
`
`tac
`
`Ser
`55
`
`act
`
`Thr
`
`aag
`
`Lys
`
`Asp
`
`aac
`
`aca
`
`aaa
`
`aat
`Asn
`
`gaa
`Glu
`
`Asn
`
`ttc
`Phe
`
`60
`
`tct
`Ser
`
`agc
`Ser
`
`aca
`
`Thr
`
`gtc
`Val
`
`tac
`
`80
`
`aag
`uys
`65
`
`
`
`:tg
`.4911
`
`gag
`Glu
`
`ctc
`Leu
`
`agc
`Ser
`
`ggc
`
`aag
`
`Gly
`
`Lys
`
`gcc
`Ala
`
`Thr
`
`:tg
`seu
`
`70
`
`:ta
`.4911
`
`
`
`act
`Thr
`
`gta
`Val
`
`gaa
`Glu
`
`aca
`
`Thr
`
`tct
`Ser
`
`gat
`Asp
`
`tcc
`Ser
`75
`
`tct
`Ser
`
`Lys
`
`gac
`
`Asp
`90
`
`tat
`
`tac
`
`ttt
`
`Tyr
`
`Phe
`105
`
`gct
`Ala
`
`atg
`Met
`
`cga
`
`Arg
`85
`
`cca
`
`Pro
`
`gtt
`Val
`
`Ala
`
`tat
`
`tac
`
`Tyr
`
`tgt
`Cys
`
`gca
`Ala
`
`aga
`
`Arg
`
`agt
`Ser
`
`gac
`
`tac
`
`Tyr
`
`tgg
`Trp
`110
`
`ggt
`Gly
`
`caa
`
`Gln
`
`gga
`
`Gly
`
`acc
`
`Thr
`
`tcg
`Ser
`115
`
`acc
`
`Val
`
`Thr
`
`gtc
`Val
`
`tcc
`Ser
`
`agt
`Ser
`120
`
`<2lO>
`<21l>
`<212>
`<2l3>
`
`13
`120
`PRT
`
`Artificial Sequence
`
`292
`
`340
`
`379
`
`

`

`<220>
`<223>
`
`Synthetic Construct
`
`<400>
`
`l3
`
`Glu
`
`Val
`
`Gln Leu Gln
`
`Gln
`
`Ser
`
`Gly
`
`Ala
`
`Glu
`10
`
`Leu
`
`Val
`
`Pro
`
`Ala
`
`Gly
`15
`
`Val
`
`Met Met Ser
`20
`
`Val
`
`Gly
`
`Thr
`
`Phe
`
`Thr
`30
`
`Pro
`
`Ile
`
`Ala Trp Met
`35
`
`Gln
`
`Val
`40
`
`His
`
`Ser
`
`Leu
`
`Glu
`
`Trp
`
`Ile
`
`45
`
`Phe His Pro
`
`Asn
`5O
`
`Ser
`55
`
`Asp
`
`Thr
`
`Asn
`
`Glu
`
`Asn
`
`Phe
`
`Gly
`
`Lys Ala Thr
`
`Lys
`65
`
`Leu
`70
`
`Thr
`
`Val
`
`Glu
`
`Thr
`
`Val
`
`Leu
`
`Glu
`
`Leu Ser Arg
`85
`
`Leu
`
`Thr
`
`Ser
`
`Asp
`
`Asp
`9O
`
`Ser
`
`Ala
`
`Val
`
`95
`
`Ala
`
`Arg
`
`Ser Asn Pro
`
`Leu
`
`Ala
`
`Met
`
`Phe
`105
`
`Gly
`
`Gln
`
`Trp
`110
`
`Gly
`
`Thr
`
`Ser Val Thr
`115
`
`Val
`
`:1
`oer
`
`120
`
`<210>
`<211>
`<212>
`<2l3>
`
`<220>
`<223>
`
`14
`16
`PRT
`
`Artificial Sequence
`
`MF2930 CDRl
`
`<400>
`
`l4
`
`Ser Tyr Pro Ile Ala Trp Met Lys Gln Val His Gly Lys
`1
`5
`10
`
`Ser Leu Glu
`15
`
`l5
`
`6 P
`
`RT
`
`Artificial Sequence
`
`<210>
`<2ll>
`<212>
`<213>
`
`<220>
`
`

`

`<223>
`
`MF2930 CDR2
`
`<400>
`
`15
`
`Asn Glu Asn Phe Lys Gly
`1
`5
`
`<210>
`<21l>
`<212>
`<213>
`
`<220>
`<223>
`
`16
`11
`PRT
`
`Artificial Sequence
`
`MF2930 CDR3
`
`<400>
`
`16
`
`Ser Asn Pro Leu Tyr Tyr Phe Ala Met Asp Tyr
`1
`5
`10
`
`<210>
`<21l>
`<212>
`<213>
`
`<220>
`<223>
`
`<220>
`<221>
`<222>
`
`17
`385
`DNA
`
`Artificial Sequence
`
`MF1849:
`
`antibody
`
`heavy chain variable region sequence of an erbB—Z binding
`
`CD8
`
`(20) .. (385)
`
`<400>
`
`17
`
`ggcccagccg gccatggcc cag gtg cag ctg gtg gag tct ggg gga ggc gtg
`
`Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val
`1
`5
`10
`
`gtc
`Val
`
`cag
`Gln
`
`cct
`Pro
`
`ggg
`
`agg
`
`Arg
`
`Gly
`15
`
`tcc
`(1
`oer
`
`ctg
`Leu
`
`aga
`
`Arg
`
`ctc tcc
`Leu Ser
`20
`
`tgt
`Cys
`
`gca
`Ala
`
`gcc
`Ala
`
`gga
`
`Gly
`
`ttc
`Phe
`
`tct
`(1
`oer
`
`25
`
`52
`
`100
`
`acc
`
`tat
`
`cac
`
`cca
`
`ggc
`
`aag
`
`148
`
`ttc
`Phe
`
`Thr
`
`agc
`Ser
`
`agt
`Ser
`
`30
`
`ggc
`
`Gly
`
`atg
`Met
`
`His
`35
`
`tgg gtc
`Trp Val
`
`cgc
`
`Arg
`
`cag
`Gln
`
`gct
`Ala
`40
`
`Pro
`
`Gly
`
`Lys
`
`ggg
`
`Gly
`
`ctg
`Leu
`
`gag
`Glu
`
`tgg
`Trp
`
`gtg
`Val
`
`gca
`Ala
`
`gtt
`Val
`
`ata
`
`tca tat
`
`Ile
`
`Ser Tyr
`
`agt
`Ser
`
`aat
`Asn
`
`aaa
`
`tac
`
`Lys
`
`tat
`
`6O
`
`gac
`
`gca
`Ala
`
`tcc
`Ser
`
`gtg
`Val
`
`aag
`
`ggc
`
`cga
`
`Gly
`
`Arg
`
`Lys
`65
`
`ttc acc
`Phe Thr
`
`atc
`Ile
`70
`
`aga
`
`gac
`
`Arg
`
`Asp
`
`aat
`Asn
`
`tcc
`Ser
`75
`
`196
`
`244
`
`

`

`acg
`Thr
`
`292
`
`tac
`
`340
`
`385
`
`agc
`Ser
`85
`
`tac
`
`ctg
`Leu
`
`aga
`
`Arg
`
`gct
`Ala
`
`gag
`Glu
`
`tct
`Ser
`
`tac
`
`Tyr
`
`Gly
`
`tct
`Ser
`105
`
`gac
`
`90
`
`tct
`Ser
`
`agt
`er
`
`no
`
`tcc
`(1
`oer
`
`gtc
`Val
`120
`
`ctg
`Leu
`
`gtc
`Val
`
`acc
`
`Thr
`
`aag
`
`Lys
`
`aac
`
`Asn
`
`acg
`Thr
`
`ctg
`Leu
`
`tat
`
`80
`
`ctg
`Leu
`
`caa atg
`Gln Met
`
`aac
`
`Asn
`
`tat
`
`gcc
`Ala
`
`gtg
`Val
`
`tgt
`Cys
`
`gca
`Ala
`
`aaa ggt
`
`gac
`
`Lys Gly
`
`gcc
`Ala
`
`ttt
`Phe
`
`gat
`
`110
`
`tgg
`Trp
`
`ggc
`
`Gly
`
`caa ggt
`Gln Gly
`115
`
`100
`
`acc
`
`Thr
`
`<210>
`<21l>
`<212>
`<213>
`
`<220>
`<223>
`
`18
`122
`PRT
`
`Artificial Sequence
`
`Synthetic Construct
`
`<400>
`
`18
`
`Gln
`
`Val
`
`Gln
`
`Leu
`
`Val
`
`Glu
`
`Ser Gly
`
`Val
`
`Val
`
`Gln
`
`Pro
`
`Gly
`10
`
`Arg
`
`Gly
`15
`
`Ser
`
`Leu
`
`Arg
`
`Leu
`20
`
`Ser
`
`Cys
`
`Ala Ala
`
`Gly
`
`Phe
`
`Thr
`
`Phe
`
`Ser
`25
`
`Ser
`
`Ser
`30
`
`Met
`
`His
`35
`
`Val
`
`Arg
`
`Gln Ala
`40
`
`Pro
`
`Leu
`
`Glu
`
`Trp
`
`Val
`
`45
`
`Ala
`
`Val
`
`Ile
`
`Sex
`
`Ala
`
`Ser
`
`Val
`
`Lys
`65
`
`Gly
`
`Arg
`
`Phe
`
`Thr
`
`Ile
`70
`
`Ser Arg
`
`Asp
`
`Asn
`
`Ser
`75
`
`Asn
`
`Thr
`
`Leu
`
`Leu
`
`Gln
`
`Met
`
`Asn
`
`Ser
`
`Leu
`
`Arg Ala
`
`Glu
`
`Thr
`
`Ala
`
`Val
`
`85
`
`9O
`
`95
`
`Ala
`
`Ser Tyr
`
`Ser
`105
`
`Ser
`
`Ala
`
`Phe
`
`Tyr
`
`Trp
`
`Asp
`110
`
`Gly
`
`Gln
`
`Gly
`ll5
`
`Thr
`
`Leu
`
`Val
`
`Thr Val
`120
`
`Ser
`
`Ser
`
`<2lO>
`<21l>
`
`l9
`5
`
`

`

`<212>
`<213>
`
`<220>
`<223>
`
`PRT
`
`Artificial Sequence
`
`MF1849 CDR1
`
`<400>
`
`19
`
`Ser Tyr Gly Met His
`1
`5
`
`<210>
`<211>
`<212>
`<213>
`
`<220>
`<223>
`
`20
`17
`PRT
`
`Artificial Sequence
`
`MF1849 CDR2
`
`<400>
`
`20
`
`Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val Lys
`1
`5
`10
`15
`
`<210>
`<211>
`<212>
`<213>
`
`<220>
`<223>
`
`21
`13
`PRT
`
`Artificial Sequence
`
`MF1849 CDR3
`
`<400>
`
`21
`
`Gly Asp Tyr Gly Ser Tyr Ser Ser Tyr Ala Phe Asp Tyr
`1
`5
`10
`
`<210>
`<211>
`<212>
`<213>
`
`<220>
`<223>
`
`<220>
`<22l>
`<222>
`
`22
`385
`DNA
`
`Artificial Sequence
`
`MF2973: heavy chain variable region
`antibody
`
`sequence of an erbB—2 binding
`
`CDS
`
`(20)..(385)
`
`

`

`<400>
`
`22
`
`ggcccagccg gccatggcc cag gtg cag ctg aag cag tct ggg gct gag ctg
`Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu
`1
`5
`10
`
`gtg agg cct ggg gct tca gtg aag ttg tcc tgc aag gct tct ggc tac
`Val Arg Pro Gly Ala Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr
`15
`20
`25
`
`att ttc act ggc tac tat ata aac tgg ttg agg cag agg cct gga cag
`Ile Phe Thr Gly Tyr Tyr Ile Asn Trp Leu Arg Gln Arg Pro Gly Gln
`3O
`35
`40
`
`gga ctt gaa tgg att gca aaa att tat cct gga agt ggt aat act tac
`Gly Leu Glu Trp Ile Ala Lys Ile Tyr Pro Gly Ser Gly Asn Thr Tyr
`45
`50
`55
`
`tac aat gag aag ttc agg ggc aag gcc aca ctg act gca gaa gaa tcc
`Tyr Asn Glu Lys Phe Arg Gly Lys Ala Thr Leu Thr Ala Glu Glu Ser
`60
`65
`70
`75
`
`tcc agc act gcc tac atg cag ctc agc agc ctg aca tct gag gac tct
`Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser
`80
`85
`90
`
`gct gtc tat ttc tgt gca aga ggg ccc cac tat gat tac gac ggc ccc
`Ala Val Tyr Phe Cys Ala Arg Gly Pro His Tyr Asp Tyr Asp Gly Pro
`95
`100
`105
`
`tgg ttt gtt tac tgg ggc caa ggg act ctg gtc acc gtc tcc agt
`Trp Phe Val Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
`110
`115
`120
`
`52
`
`100
`
`148
`
`196
`
`244
`
`292
`
`340
`
`385
`
`<210>
`<211>
`<212>
`
`23
`122
`PRT
`
`<213> Artificial Sequence
`
`<220>
`
`<223> Synthetic Construct
`
`<400>
`
`23
`
`Gln Val Gln Leu Lys Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala
`1
`5
`10
`15
`
`Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Ile Phe Thr Gly Tyr
`20
`25
`30
`
`Tyr Ile Asn Trp Leu Arg Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile
`35
`40
`45
`
`

`

`Ala Lys
`50
`
`Ile
`
`Pro
`
`Gly
`
`Ser Gly Asn Thr
`55
`
`60
`
`Asn
`
`Glu
`
`Phe
`
`Arg Gly
`65
`
`Ala
`
`Thr
`
`Leu
`
`Thr Ala Glu Glu
`
`Ser
`
`Ser
`
`Ser
`
`Thr
`
`Ala
`
`70
`
`75
`
`Tyr
`80
`
`Met Gln
`
`Leu
`
`Ser
`
`Leu
`
`Ser
`85
`
`Thr Ser Glu Asp
`9O
`
`Ser
`
`Ala
`
`Val
`
`Phe
`95
`
`Ala Arg
`
`Gly
`
`His
`
`Pro
`100
`
`Asp Tyr Asp Gly
`105
`
`Pro
`
`Trp
`
`Phe
`
`Val
`110
`
`Trp
`
`Gly Gln
`
`Gly
`115
`
`Thr
`
`Leu
`
`Val
`
`Thr Val Ser Ser
`120
`
`<210>
`<211>
`<212>
`<213>
`
`<220>
`<223>
`
`24
`16
`PRT
`
`Artificial Sequence
`
`MF2973 CDRl
`
`<400>
`
`24
`
`Gly Tyr Tyr Ile Asn Trp Leu Arg Gln Arg Pro Gly Gln Gly Leu Glu
`1
`5
`10
`15
`
`25
`
`6 P
`
`RT
`
`Artificial Sequence
`
`MF2973 CDRZ
`
`<210>
`<211>
`<212>
`<213>
`
`<220>
`<223>
`
`<400>
`
`25
`
`Asn Glu Lys Phe Arg Gly
`1
`5
`
`<210>
`<211>
`<212>
`<213>
`
`<220>
`<223>
`
`26
`13
`PRT
`
`Artificial Sequence
`
`MFl973 CDR3
`
`<400>
`
`26
`
`