W0 03/004704
`
`PCT/NL02/00390
`
`90
`
`FIG 25. U-Z OS/Tet—Off/leXA—HPl cells were transfected With candidate
`
`Arabidopsis STAR elements and cultivated at low doxycycline concentrations.
`
`Total RNA was isolated and subjected to RT~PCR; the bands corresponding to
`
`the zeocin and hygromycin resistance mRNAs were detected by Southern
`
`blotting and quantified with a phosphorimager. The ratio of the zeocin to
`
`hygromycin signals is shown for transfectants containing zeocin expression
`
`units flanked by 12 different Arabidopsis STAR elements, the Drosophila scs
`
`element, or no flanking element.
`
`10
`
`Figure 26
`
`Sequences comprising STARl —— STAR65 (SEQ IDzl — 65)
`
`Sequences comprising STAR66 and testing set (SEQ ID266 — 84),
`
`Sequences comprising Arabidopsis STAR A1-A35 (SEQ ID:85-119).
`
`
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