1/65
`
`Fig“ i
`
`ffim
`
`SAMPLE
`
`: NNENANANNN
`
`:
`
`AAA
`
`NNQNLET
`
`’ NENENATaNN
`
`
`
`
`
`‘“““““““““““““““
`
`““““““““
`
`QETEQTfiQN
`
`
`
`.“““““““““““““““
`
`“““““.“““5:6
`
`QATA
`
`ANALYSES
`
`,
`
`
`
`Fig“ i
`
`ffim
`
`SAMPLE
`
`: NNENANANNN
`
`:
`
`AAA
`
`NNQNLET
`
`’ NENENATaNN
`
`
`
`
`
`‘“““““““““““““““
`
`““““““““
`
`QETEQTfiQN
`
`
`
`.“““““““““““““““
`
`“““““.“““5:6
`
`QATA
`
`ANALYSES
`
`,
`
`
`
`2/65
`
`
`
`
`
`mwmmmhmdfl
`mmmmgxz‘qfigMzgguwammzagfimfimgAAAAAAAAAAAEmma”figsfimm
`
`
`
`
`
`Nfi.WEwzszEszumy?“
`
`g8.flmfizmmxaMafia3%mmgma
`
`wszQMMmzm
`
`
`
`mUWQmmfihzmwaamhmzm
`
`
`
`
`
`MQimnumwzmflag.mmfifimhzav
`
`
`
`
`
`“““““““““““““““““swfiwgmmxhmmmmmawmfifl
`
`
`
`Mwamanmmmamafimfiw
`
`
`
`mmfigmammmmd
`
`$me
`
`muggy”
`
`magnumH
`
`
`“gmmmwmgm
`
`
`
`
`4/65
`
`
`
`
`
`m§§SEQ
`
`
`
`wfifimmmwhfiammm
`
`wmmH“““““““““““““““““““““““““““““““““““““““““H“““““““““““““““““““““““““““““““““““““““‘
`
`H 5/65
`
`whmgwmammw
`
`
`I“““““““““““““4aaaaaaaaagageafiseaefiM
`ammmwm3mm“.@an
`mmaaww Awfifimmgfium
`.mefi‘‘
`
`mmm‘uw“““““““““““““““““““““““““““““““““““““““““““““““““““““““““““““‘,mammk
`
`
`
`
`ffiggs§3
`
`6/65
`
`322
`
`K»Q1Q
`Q “““““““““““““““$13“
`
`
`Q
`Q
`'TRANSPQRT
`314
`§““““%
`’GENERAWE§““““Q§ QQQRE
`QQNQENTRQQE
`QRQPLETS>
`
`
`TQ
`,““““Q,
`QRQPLEng “ggRgpLETSE _
`QRQPLE?S
`’ REAQTEGN
`'
`
`
`
`
`
`SUE
`2% QQQ
`LgQQQQQQQQ REAST
`'
`‘mETEQT ”DRQPLETS
`
`
`
`
`
`
`
`’REQERVQQQ
`
`fTRANSFERi
`v QRQPLETQ§
`¥RGM ;
`
`56838
`
`
`
` ‘
`
`‘_____ REACTEQN »
`
`QQQ"
`
`
`
`W65
`
`
`928
`
`‘
`‘
`E
`,DE?ECTQR§
`I
`mwmwmwm:“““““““3??
`qugygy
`g
`
`,
`
`g
`§,934
`
`‘
`
`§§fl
`
`Q58
`
`
`a
`+
`x
`£3§5§§§T£
`‘32
`“““““““““““““““““
`g
`9-. M
`g §RQPLET g
`g
`‘
`gQENEfiEGRg
`“““““““““““““““““l—““““1
`
`g
`g
`
`iPRESSQRE’
`gamma
`
`
`
`
`
`Fig“ 15
`
`
`
`miW ”3935 mm 1655
`W
`i
`I
`AijTQSAMpLER ““““
`
`WWWWWWN
`SETE
`
`““““
`
`WASTE!
`““““CQLLECHQN
`
`W85Q
`
`W163i)
`
`
`
`Figs,
`
`E. §
`
`103%
`\&
`’gfiy‘i
`
`”36??
`
`f :31“-
`
`W65
`
`1935
`\
`
`FACKET 2 (1:36:22)
`
`“E 068
`
`’E {355
`
`WELL3
`
`WELL as
`
`QACKET 3 (”3 $64)
`
`‘zfiwfwE‘§
`aw.,Xfig”?Um
`
`
`
`
`
`
`3.9/55
`
`
`
`
`
`E3565
`
`
`
`
`
`3.2/55
`
`
`Nfifigflmmmk
`
`\gmmfimammxww
`
`mzfimmafifimma
`
`
`
`
`
`
`méa
`
`1239
`
`1239€252
`
`12301244 1242
`
`
`
`3.4/55
`
`
`
`1284
`
`{’3 272
`
`
`
`
`
`
`
`
`
`3.6/55
`
`WWW”
`
`
`
`3.7/55
`
`
`
`
`
`EMAGER
`“3338 13%
`
`M 382
`
`
`
`3.8/55
`
`
`
`1433
`
` AAAAAAA
`
`144a
`
`1444
`
`v/
`
`AAAAAAA
`
`VALVE
`
`”$4355
`K
`
`
`
`AAAAAAAA
`
`@448
`
`WASTE;
`CQLLECTEQN
`
`K‘MSQ
`
`
`
`
`‘
`
`fiNJECTEQN “““““
`VALVE
`
`
`‘
`
`
`
`
`‘ CGLLEQTKEN
`
`3.9/55
`
`AUTQSAMPLER
`
`““““
`
`
`
`
`
`29/55
`
`mmww/y
`
`ggum
`
`..mung,
`
`@233QO
`
`
`
`“““““““““
`
`
`
`$.33,@zmmnommm
`
`gag?“
`
`mfimm
`
`
`
`
`
`zefimgugggzmgmmmggmag
`
`‘
`
`
`mgg”23$me,gmfi@30szmeWmmgmmgfimgg
`
`
`
`
`
`
`
`
`
`
`
` m QTHER
`
`REAGENT QHAMEER$
`
`
`
`22/55
`
`1?28
`
`by
`
`K
`3:
`
`”a
`
`g
`
`V
`
`Figs 4§
`
`1?4%d 1?4Qe
`mm
`1740b"? ?4§§;/\ ;
`"gig “W429
`”flfffiW
`
`j‘maak
`
`I mm
`s“ ”””””
`
`g: Manama ““““
`
`Fig 4324
`12?G 1?36
`
`1?38
`
`1?28
`
`
`}0.
`
`,ea
`
`€744
`
`:17498
`
`”HM-0b
`1?4QC
`
`1?4Qۤ
`
`1?4Q$
`
`
`
`
`
`23/55
`
`
`“32749
`“EM-{3&3 3“}?4QQ 12’4Gd 1?4Ge
`
`
`
`
`
`
`
`Fig, 48%
`
`fig 48C
`
`{189%
`
`1815\
`
`
`
`
`
`2fi/55
`
`
`
`REAGEggCEEfiBERS “315% §
`N: 912
`
`
`
`26/55
`
`$thE.
`
`mmmmS85
`
`
`
`
`
`N.mammmaéuEmmfim
`
`
`
`27/55
`
`
`"Pm“ \
`Him“
`
`
`
`~88Q8888
`REAGENTPUMPS
`
`
`
`338188838188838188cfi2188 §
`8
`8
`88888 81888 81888 81888 88888§
`
`
`
`
`
`
`28/55
`
`
`
`
`
`29/55
`
`
`
`
`
`
`
`
`
`
`
`
`
`32/55
`
`mt“.p
`
`MMMm,_m““_m_w_mM_nnnflfl__‘_‘~
`N\\\\\\\\\§§%‘§\\\\\\‘N\\$\VN\\_.\\\\\‘\\\\\V
`
`
`
`a
`
`“a-. ‘3.
`a5
`a
`0
`5:
`2““ g I I» g»
`H
`d.
`9“
`as
`Q,“
`"
`9a
`.9“ 5
`l
`I
`s
`5
`
`n :
`
`3
`3
`‘
`
`i
`a
`s
`a
`
`
`
`f
`
`mI‘
`
`m
`
`
`
`3fi/55
`
`“
`'
`Fag“ ”$3
`
`Efiflfi
`
`if
`
`31%
`
`PRQVEQENQ A HGT-“START TEM?ERATURE TS A FLUifi
`
`PRQVESENQ A QENATURENG TEM?ERATURE ”K? A FLEJEEIE
`
`TEMPERATfiRE TQ “THE FLUKE
`
`PRQVEQENG A FQLYMERASE EXTENSEQN
`
`
`
`36/55
`
`
`
`
`
`12:: m
`
`a\ x
`
`32%8
`
`
`
`\ {w
`
`3228 ~
`
`’ 3234
`
`
`
`39/55
`
`Fig, 728
`
`3238
`LAMNAL
`
`:E
`
`:
`
`
`
`49/55
`
`Fig 8
`
`$5 ““““““““““““““““““““““““““““““““““ ““““““““““““““““““““““““““““““““““““““““““““““““““““““““““
`
`
`
`"fEMPERATURE(QC) “5m
`
`m2:3
`
`"WEQ
`
`AVERAGE
`
`Fmaa
`
`VELQCEW
`
`5%
`
`:::
`
` $5 ‘ ‘
`
`
`9.32
`9.633
`3&4
`(3.05
`@336
`ARC—LENGTH (RAWANS;
`
`
`
`4E/55
`
`
`
`
`
`42/55
`
`ANNEAL
`
`
`
`EXTENQ
`
`
`
`Mfififigfikm.
`
`3256§\\_
`
`
`
`
`
`3
`Fig 843333;;
`MELT 3'3
`
`3
`
`33/33
`
`33333
`K”3:233 33333 3
`ANNEAL 3|3 3/32133 EXTENE
`
`333333
`
`K32GGH33333 3
`
`Fig 8%333443
`
`333233
`
`{3206333333
`
`Fig 8§333333
`
`
`
`
`
`
`
`44/55
`
`
`7 3213f EXTENQ
`
`3%255f
`
`325535
`
`£32991?
`
`
`
`{32mg
`
`“255$:
`
`3%256g
`(32%;: EXTENfi
`
`
`
`
` 3334“
`
`
`
`a
`Figs: 8%
`
`3254?}
`
`32583
`
`“
`
`4&‘55
`
`{329951
`
`
`
`
`
`
`
`
`
`
`
`48/55
`
`WFLUQRESQENCE »
`
`T§ME{$)
`
`
`
`
`
`
`
`
`
`
`
`51/55
`
`Figs 1 SQ
`
`QQ44
`
`Fig“ E. {E E.
`
`
`445%
`
`4455K?“
`
`
`
`
`52/55
`
`
`
`
`
`53/55
`
`
`
`‘LN,~AX
`a‘<>§3
`NEfifém
`efy
`
`4562
`
`4554
`
`$4553¢fl
`
`§
`i
`;
`
`f”
`4585
`
`IIIIIIIIIIIIII I;
`fifilfiflfiflflflfifilfi.fifi
`Ills-lllllg-IIII
`flfiflfllflflfiflflg‘fiflflflflfii o
`lulu-A-l-mfiullnm
`
`o
`
`6’
`
`
`
`54/55
`
`EXTRACT TARGET ENFORMA‘E‘BQN
`
`
`
`GQMPARE EEETERMENEB GONFEDENQE CQNBENQN
`““““““““““““““““““
`““““““““““““““““““
`
`
`
`gamma
`
`wamffiaia
`
`CQNHEENSE CQNmTaQN
`
`
`
`
`I
`
`ATTAENEQ
`
`
`
`
`QRQPLET
`“““ W;;;‘GENERA?§QN PARAMETER
`
`AmuSTMENT
`
`
`
`
`»~4822
`3
`ADJUST MRQLET
`
`PARAMETERS)
`
`“““““
`
`TARGET MQLECULE CQNCENTRATEQN
`
`
`
`56/55
`
`figs 1 Q8
`
`3&6;
`
`§mmwMEAsUREm§
`;
`§fiS$QQRAM§i
`
`COU »»»»»»»»»»»»»:3:»»»»»a.
`
`
`?é@
`6&3
`25%
`3am
`4Q
`fiéfi
`MEASQRE QF iNTENSEW
`
`aéfi
`
`90%
`
`Figs: mg
`
`339;
`
`3
`:meEAsuREQ
`HESTQGRAM .
`
`3
`
`g
`
`5
`
`immfiTGRfiEfizi
`...... FET QRQER 4
`i““fiTQRfiER?5
`
`g
`
`Sm
`32mgé
`CQU
`
`,
`:3
`
`
`
`
`
`5&0
`85%
`?§a
`afifi
`QGG
`23%
`3am
`43%
`MEASQRECEfiNTENSfiY
`
`
`
`LEEREEEQUALS
`
`57/55
`
`fl LMS RESEQUALS
`
`{mm
`
`am;
`
`{mag
`
`2%
`
`CQLLECT mm mam QRQPLETS
`
`‘«4§$§
`
`4mm
`
`QETERMENE THE AQTUAL ERAGTTQN 0E
`
`TARGETwQQNTAENTNQ ERQELETS FRGM EATA
`
`, ESTEMATE TARQET CQNCENTRATEQN BY MENEMEZTNG A MEASURE
`GE THE EEEEERENCE BETWEEN THE THEQRETECAL ERACTEGN
`
`
`
`
`
`AME} THE FRACTEQN EETERMENEE ERQM THE EATA
`
`
`
`Fig. i m
`
`58/55
`
`5?13
`K»
`
`5mg
`~
`NEéATEVE
`
`
`
`SEGNAL ~~~~~~~~~~~~~~
`
`57%
`P SETEVE
`W26
`FAL§E
`NEQATEVE
`
`»
`
`:
`
`5mg
`FALéE
`PQSETEVE
`5M4
`
`+
`f
`
`~~~~~~~~~~~~~ ; ~~~~~~~~~~ “mm-THRESHGLQ
`
`—THRESHQLQ
`
`CAMBRATEQN ...........
`REAQENTS
`
`{WA .........
`5275*?
`“59“».ANALYZER
`
`
`
`
`
`Figs: 1 E S
`ETQE
`55232 {3ng
`
`5784
`
`5?88
`CHANN E... E
`
`
`‘5?95
`
`KSTQE
`
`ESTES
`
`QU NCHER
`
`
`
`ETQG
`
`
`
`{3H NNEL 2
`
`
`
`
`
`
`
`
`
`FQRWARQ
`
`
`
`
`‘EE
`
`PRQBE:
`
`STQAEX
`
`
`
`
`
`. EREMERE:
`
`
`
`
`REVERSE
`
`2 FALSE
`
`(58E 3
`
`,
`
`mama.
`
` Fig E w
`EQSETEVES
`(CHANNELE)
`
`{WEmE
`
`wwmmmvwwww—"fihTHRESE—EQLB
`EgE
`EEEEE 5314
`
`
`SEE
`EEEEE
`EEEEEEEEEE
`
`
`TRANSFQRM g x
`TEME
`25%;
`Eg
`{5812
`
`
`
`(CHANNEL2)
`$EGNAL
`
`
`
`
`
`
`
`EEEEE
`
`EEEEEE2EEEEEEE
`
`EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
`
`{5818
`{EFALSE
`PQSETEVES
`
`“““
`
`Q
`
`L”
`
`0:1:
`
`
`EEEEEE
`
`
`
`
`
`Fig” i .3 g
`535%
`
`535:2
`TfiQN
`
`CALEB
`
`5854
`T ST
`
`KW» F—A_W
`:
`: Rf5858
`§ §§ 5§§Q
`53642 5862
`SEGNAL
`
`4/
`
`5885
`
`EALSé:
`
`(T53?)
`
`MQRMALEZEB3563ka
`
`5890
`
`
`
`Eggs E. 2 E»
`
`‘ 5‘?56
`
`SAMFELE
`
`““““““““““
`
`
`
`;
`‘
`
`i
`i
`
`;
`
`i
`s
`
`AMPLaFaaAfiQN
`REAGENTS
`_
`
`QGNTRGL
`AMPUFKAHQN
`
`REAGENTS
`
`§
`
`CQNTRQL
`
`TEWLATE
`
`i
`
`[5?43
`{591%
`
`{5-244
`g
`
`{5mg
`g
`
`‘
`
`{5mg
`g
`
`“““““““
`
`““““““““
`
`““““““““ m.
`
`““““““““““““““““““““““““““““““““““““““‘
`
`53755Figs E 22 {5?4Q
`
`
`
`
`
`REAGENTS
`‘““““““““““““
`‘“““““““““““““
`
`
`
`CQNTRQL
`AMPLEFECATEQN
`,,,,,,,,,,,,,,,,,, 2
`
`
`REAGENTS
`
`
`[5&23
`
`{53?44
`
`{5?45
`
`{5748
`
`AMPLEFiCATEQN
`
`“““““““133;”:
`
`““““““““a»
`
`‘33“
`
`Figs 1 2.3
`
`FRQBE E:
`
`Vi}:
`
`3333::
`
`AK«$92
`mFGRWARB‘S
`REVERSE 1
`
`PNMERSE:
`
`PRGBE 2;
`
`““““““““““
`
`53388
`CH NNEL “E
`5?58\
`5““Nflwgi QUEN6§ER1
`
`5?9a
`
`
`
`
`5?§a
`
`
`CH NNELZ {5933;
`
`
`5933 i
`5”35\QYE2 QuENaHééz
`%
`
`
`
`
`g
`
`
`
`
`~mmmmmFQRWARQ2 §
`:
`
`
`PREMERS.
`REVERSE 2
`
`
`
`
`62/55
`
`Fig“ E 24
`
`Fig“ E253
`
`iNPflBfFKEN:
`
`
`
`Ni§§N§HBY§K§NZ
`
`5§4Q
`
`
`
`
`5944
`\
`
`NQEEST
`
`$fi3$¥ffifl§8
`
`
`
`NQTES?
`
`PQSWEVES
`
`
`
`
`
`
`TfifiE
`
`NQ QQNTRQL
`
`mamvgg
`
`Figs 1 2%
`5755
`g
`:- '
`§
`SAMPLE
`g““““““““E
`>
`
`{5?44
`i
`g
`
`fiffigéfi
`
`{596%
`
`AMPLEFECATiQN
`REAGENTS
`
`i
`
`>
`
`g
`
`{5?45
`
`{5‘34
`
`:
`REAGENTS
`
`CQNTROL
`
`AMPUFmfifiQN
`
`§
`
`§,,,,i
`
`QQNTRQL
`
`TEmaaLATE
`
`émw£
`
`'
`
`i
`
`E
`
`
`
`63/55
`
`
`
`
`
`
`60:2
`
` {EYE ”E 4* {EYE 2
`MEXQETAHQN MGM”?
`8014
`
`6&22
`
`
`
`SENQLEA
`“6929
`EXQETATEQN
`3 K5?48
`SQTEQS
`3
`
`
`5943
`
`565i)
`FggLSE
`N EGATEVE
`
`TRANSFQRM
`
`
`
`6&‘55
`
`TRANSFQRM
`
`
Accessing this document will incur an additional charge of $.
After purchase, you can access this document again without charge.
Accept $ Charge
This document could not be displayed.
We could not find this document within its docket. Please go back to the docket page and check the link. If that does not work, go back to the docket and refresh it to pull the newest information.
Your account does not support viewing this document.
You need a Paid Account to view this document. Click here to change your account type.
Your account does not support viewing this document.
Set your membership
status to view this document.
With a Docket Alarm membership, you'll
get a whole lot more, including:
- Up-to-date information for this case.
- Email alerts whenever there is an update.
- Full text search for other cases.
- Get email alerts whenever a new case matches your search.
One Moment Please
The filing “” is large (MB) and is being downloaded.
Please refresh this page in a few minutes to see if the filing has been downloaded. The filing will also be emailed to you when the download completes.
Your document is on its way!
If you do not receive the document in five minutes, contact support at support@docketalarm.com.
Sealed Document
We are unable to display this document, it may be under a court ordered seal.
If you have proper credentials to access the file, you may proceed directly to the court's system using your government issued username and password.
Access Government Site
