Abstract
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`Method of performing a multiplexed digital assay. The method may include (1)
`
`partitioning a sample containing at least two distinct targets into droplets; (2) amplifying
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`the at least two distinct targets within the droplets; (3) detecting light from the droplets
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`indicative of the presence or absence of each distinct target in each droplet; and (4)
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`calculating a level of each distinct target based on the detected light.
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`253
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