said analyte to generate an optically detectable signal; and measuring said optically detectable signal with a plurality
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`of detection spectral regions, wherein the presence of said optically detectable signal within a dynamic range of at
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`least one detection spectral region is indicative of the concentration of said analyte in said sample of biological fluid.
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`In an embodiment, the measuring is performed by an imaging device configured to measure a plurality of detection
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`spectral regions. In another embodiment, the imaging device is configured to measure the plurality of detection
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`spectral regions simultaneously. In another embodiment, the imaging device is configured to measure the plurality
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`of detection spectral regions sequentially.
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`[0023]
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`An aspect of the invention provides a method for increasing the accuracy of an assay comprising
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`imaging a sample in a first tip to determine the volume of the first sample; imaging one or more reagents in a second
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`tip to determine the volume of the one or more reagents; mixing the sample and the one or more reagents to form a
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`reaction mixture; imaging the reaction mixture; correcting a calibration based on said determined volumes of the
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`sample and the one or more reagents; and calculating a concentration of an analyte using the corrected calibration.
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`In an embodiment, the method further comprises imaging the reaction mixture to determine the volume of the
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`reaction mixture. In another embodiment, the imaging of the sample in the first tip is conducted using a camera
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`configured to capture a side profile of the first tip. In another embodiment, imaging of the one or more reagents in
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`the second tip is conducted using a camera configured to capture a side profile of the second tip. In another
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`embodiment, the height of the sample and the one or more reagents is calculated based on the captured profiles. In
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`another embodiment, determining the volume is based on the height of the sample and the one or more reagents and
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`the known cross-sectional areas of the sample and the one or more reagents respectively. In another embodiment,
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`the calibration is also based on the determined volume of the reaction mixture.
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`[0024]
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`Another aspect of the invention provides a setup, comprising: a vessel configured to accept and confine
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`a sample, wherein the vessel comprises an interior surface, an exterior surface, an open end, and an opposing closed
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`end; and a tip configured to extend into the vessel through the open end, wherein the tip comprises a first open end
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`and second open end, wherein the second open end is inserted into the vessel, wherein the vessel or the tip further
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`comprises a protruding surface feature that prevents the second open end of the tip from contacting the bottom of the
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`interior surface of the closed end of the vessel. In an embodiment, the surface feature is integrally formed on the
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`bottom interior surface of the vessel. In another embodiment, the surface feature comprises a plurality of bumps on
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`the bottom interior surface of the vessel. In another embodiment, the protruding surface feature is at or near the
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`closed end.
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`[0025]
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`Another aspect of the invention provides a sample processing apparatus comprising a sample
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`preparation station, assay station, and/or detection station; a control unit having computer-executable commands for
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`performing a point-of-service service at a designated location with the aid of at least one of said sample preparation
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`station, assay station and detection station; and at least one centrifuge configured to perform centrifugation of a
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`sample from a fingerstick. In an embodiment, the centrifuge is contained within the sample preparation station
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`and/or the assay station. In another embodiment, the computer-executable commands are configured to perform the
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`point-of—service service at a site selected from the group consisting of a retailer site, the subject’s home, or a health
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`assessment/treatment location.
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`4752259
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`-5-
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`WSGR Docket No. 30696—733201
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`