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SECOND SUBSTITUTE AMENDMENT AND RESPONSE UNDER37 C.F.R. § 1.111
`Serial Number: 13/247,241
`Filing Date: September 28, 2011
`Re-issue of U.S. Patent No. 7,670,495
`
`Page 2
`Dkt: 3406.005USR
`
`IN THE CLAIMS
`
`1. (Allowed) A method for treating waste water comprising:
`
`providing a flow-through oxygenator comprising an emitter for electrolytic generation of
`
`microbubbles of oxygen comprising an anode separatedat a critical distance from a cathode and
`
`a powersourceall in electrical communication with each other,
`
`placing the emitter within a conduit; and
`
`passing waste water through the conduit.
`
`Claims 2-12 are cancelled.
`
`13. (New) A methodfor producing an oxygenated aqueous composition comprising:
`
`flowing water at a flow rate no greater than 12 gallons per minute through an electrolysis
`
`emitter comprising an electrical power source electrically connected to an anode electrode and a
`
`cathode electrode contained in a tubular housing,
`
`causing electricity to flow from the power source to the electrodes, and,
`
`producing the composition comprising a suspension comprising oxygen microbubbles
`
`and nanobubbles in the water, the microbubbles and nanobubbles having a bubble diameter of
`
`less than 50 microns, wherein:
`
`the anode electrode is separated at a critical distance from the cathode
`
`such that the critical distance is from 0.005 inches to 0.140 inches;
`
`the power source produces a voltage no greater than about 28.3 volts and
`
`an amperage no greater than about 13 amps,
`
`the tubular housing has an inlet and an outlet and a tubular flow axis from
`
`the inlet to the outlet:
`
`
`the water flowsin the inlet, out the outlet,
`is in fluid connection with the
`
`electrodes, and the water flowing into the inlet has a conductivity produced by the
`
`presence of dissolved solids such that the water supports plant or animallife.
`
`

`

`SECOND SUBSTITUTE AMENDMENT AND RESPONSE UNDER37 C.F.R. § 1.111
`Serial Number: 13/247,241
`Filing Date: September 28, 2011
`Re-issue of U.S. Patent No. 7,670,495
`
`Page 3
`Dkt: 3406.005USR
`
`14. (New) A method according to claim 13 wherein the housing contains at least one anode and
`
`at least one cathode, the electrodes are of a grid or solid design and are relatively positioned in
`
`cross section along the radius of the tubular housing with their long axes substantially parallel to
`
`the tubular water flow axis of the housing.
`
`15. (New) A method according to claim 13 wherein the housing has a side arm positioned at an
`
`angle relative to the tubular flow axis and the electrodes are located in the side arm.
`
`16. (New) A method according to claim 15 wherein the side arm contains a multiple numberof
`
`anode and cathode electrodes and the electrodes are plate shaped.
`
`17. (New) A method according to claim 14 wherein a multiple number of anode and cathodes
`
`are present andare of grid or solid design.
`
`18. (New) A method according to claim 13 wherein the water has a temperature no greater than
`
`about ambient temperature at the inlet and the water temperature is a factor for formation of the
`
`suspension.
`
`19. (New) A method according to claim 13 wherein the microbubbles and nanobubbles remain
`
`in the water at least in part for a period up to several hours.
`
`20. (New) A method according to claim 19 wherein the period for which the microbubbles and
`
`nanobubblesat least in part remain in the water is determined by containing the water with
`
`microbubbles and nanobubbles in a two and one half gallon aquarium reservoir container.
`
`21. (New) A method according to claim 13 wherein the microbubbles and nanobubbles
`
`supersaturate the water.
`
`22. (New) A method according to claim 13 wherein the bubble diameter of the microbubbles
`
`and nanobubblesis less than 0.0006 inches.
`
`

`

`SECOND SUBSTITUTE AMENDMENT AND RESPONSE UNDER37 C.F.R. § 1.111
`Serial Number: 13/247,241
`Filing Date: September 28, 2011
`Re-issue of U.S. Patent No. 7,670,495
`
`Page 4
`Dkt: 3406.005USR
`
`23. (New) A method according to claim 13 wherein the separation of electrodes is maintained
`
`by a nonconductive spacer.
`
`24. (New) A method according to claim 13 wherein the electrode separation distance is about
`
`0.045 to about 0.06 inches.
`
`25. (New) A method according to claim 13 wherein the microbubbles and nanobubblesare
`
`substantially incapable of breaking the surface tension of the water.
`
`26. (New) A method according to claim 13 wherein each anode and cathode electrode ofthe
`
`emitter is positioned so that substantially all points midway between opposing anode and cathode
`
`electrodes are closer to a surface of the tubular housing than to a center point within the tubular
`
`housing.
`
`27. (New) A method according to claim 26 wherein each anode and cathode electrode of the
`
`emitter are positioned so that the electrodes do not obstruct a water flow passage along the center
`
`of the tubular housing.
`
`

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