`
`This listing of claims will replace all prior versions and listings of claims in the application.
`
`Listing of Claims
`
`1. (Currently Amended) A coating composition, comprising a coating that comprises an active
`
`enzyme, wherein the coating comprises an architectural coating, an automotive coating, a can
`
`coating,-a—sealant—eeati-ng; a chemical agent resistant coating, a camouflage coating, a traffic
`
`marker coating, an aircraft coating, 9La nuclear power plant coatingrer—a—eembinatien-thereef,
`
`wherein the active enzyme comprises an antimicrobial enzyme, a peptidase, an esterase, a
`
`petroleum lipolytic enzyme, or a combination thereof, and wherein the coating comprises an
`
`antimicrobial agent comprising an antimicrobial peptide, wherein the antimicrppial peptide
`
`comprises a peptide seguence of SEQ ID No. 40 or a fpnctionally eguiyplent cpnservative,
`
`
`
`more than a £2 difference in hydropathic value of thp the-Doolittle scale relative thereto.
`
`2. (Original) The coating of claim 1, wherein the antimicrobial enzyme comprises a lysozyme, a
`lysostaphin, a libiase, a lysyl endopeptidase, a mutanolysin, a cellulase, a chitinase, an a—agarase,
`
`an B-agarase, an N-acetylmuramoyl-L-alanine amidase, a lytic transglycosylase, a glucan endo-
`
`l,3-B-D—glucosidase, an endo-],3(4)-B-glucanase, a B—lytic metalloendopeptidase, a 3-deoxy-2-
`
`octulosonidase, a peptide—N4—(N-acetyl-B-glucosaminyl)asparagine amidase, a mannosyl-
`
`glycoprotein endo-B-N-acetylglucosaminidase, a t-carrageenase, a K-carrageenase, a X-
`
`carrageenase, an a-neoagaro-oligosaccharide hydrolase, an endolysin', an autolysin, a
`
`mannoprotein protease, a glucanase, a mannase, a zymolase, a lyticase. a lipolytic enzyme, or a
`
`combination thereof.
`
`3. (Original) The coating of claim I, wherein the antimicrobial enzyme comprises a lysozyme.
`
`4. (Withdrawn) The coating ofclaim 1, wherein the antimicrobial enzyme comprises a
`
`lysostaphin.
`
`3/61
`
`
`
`5. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a libiase.
`
`6. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a lysyl
`
`endopeptidase.
`
`7. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`mutanolysin.
`
`8. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`cellulase.
`
`‘:
`9. (Withdrawn) The coating of claim 8, wherein the cellulase comprises a a-cellulase.
`
`10. (Withdrawn) The coating of claim 8, wherein the cellulase comprises a B-cellulase.
`
`ll. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`chitinase.
`
`12. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises an a-
`
`agarase.
`
`‘ l3. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises an [3-
`
`agarase.
`
`l4. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises an N-
`
`acetylmuramoyl-L-alanine amidase.
`
`15. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises a lytic
`
`transglycosylase.
`
`4/61
`
`
`
`16. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a glucan
`
`endo-l ,3-B-D-glucosidase.
`
`17. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises an endo-
`
`.
`
`l,3(4)-B-glucanase.
`
`18. (Withdrawn) The coating ofclaim 1, wherein the antimicrobial enzyme comprises a B-lytic
`
`metalloendopeptidase.
`
`l9. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises a 3-
`
`deoxy-2-octulosonidase.
`
`20. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises a
`
`peptide-N4-(N-acetyl-B-glucosaminyl)asparagine amidase.
`
`21. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`mannosyl-glycoprotein endo-B-N-acetylglucosaminidase.
`
`22. (Withdrawn) The coating ofclaim 1, wherein the antimicrobial enzyme comprisesa 1—
`
`carrageenase .
`
`23. (Withdrawn) The coating of claim l, wherein the antimicrobial enzyme comprises a K-
`
`carrageenase .
`
`24. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a X-
`
`carrageenase.
`
`25. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises an a-
`
`neoagaro-oligosaccharide hydrolase.
`
`5/61
`
`
`
`26. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises an
`
`endolysin.
`
`27. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises an
`
`autolysin.
`
`28. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`mannoprotein protease.
`
`29. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`glucanase.
`II
`
`30. (Withdrawn) The coating of claim I, wherein the antimicrobial enzyme comprises a
`
`mannase.
`
`3 l. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`zymolase.
`
`32. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`lyticase.
`
`33. (Withdrawn) The coating of claim 1, wherein the antimicrobial enzyme comprises a
`
`lipolytic enzyme.
`
`34. (Withdrawn) The coating ofclaim 33, wherein the lipolytic enzyme comprises a
`
`phospholipase.
`
`35. (Previously Presented) The composition of claim I, wherein the antimicrobial enzyme
`
`catalyzes a reaction that degrades at least one member selected from the group of a cell wall and
`
`cell membrane.
`
`6/61
`
`
`
`36. (Withdrawn) The composition of claim 1, wherein the esterase comprises a lipolytic
`
`enzyme, a sulfuric ester hydrolase, a phosphoric triester hydrolase, or a combination thereof.
`
`37. (Withdrawn) The composition of claim 1, wherein the peptidase possesses esterase activity.
`
`38. (Withdrawn) The composition of claim 1, wherein the active enzyme comprises a plurality
`
`of active enzymes.
`
`39. (Withdrawn) The composition of claim 1, wherein the coating comprises an interior
`
`coating.
`
`40. (Withdrawn) The composition of claim 36, wherein the lipolytic enzyme comprises a
`
`carboxylesterase, a lipase, a lipoprotein lipase, an acylglycerol lipase, a hormone-sensitive
`
`lipase, a phospholipase A], a phospholipases A2, a phosphatidylinositol deacylase, a
`
`phospholipase C, a phospholipase D, a phosphoinositide phospholipase C, a phosphatidate
`
`phosphatase, a lysophospholipase, a sterol esterase, a galactolipase, a sphingomyelin
`
`phosphodiesterase, a sphingomyelin phosphodiesterases D, a ceramidase, a wax—ester hydrolase,
`
`a fatty-acyl-ethyl-ester synthase, a retinyl-palmitate esterase, a 1 l-cis-retinyl-palmitate
`
`hydrolase, an all-trans-retinyl-palmitate hydrolase, a cutinase, an acyloxyacyl hydrolase, or a
`
`combination thereof.
`
`41. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`combination of lipolytic enzymes.
`
`42. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`carboxylesterase derived from Actim'dia deliciosa, Aedes aegypti, Aeropyrum perm‘x,
`
`Alicyclobacillus acidocaldarius, Aphis gossypii, Arabidopsis thaliana, Archaeoglo'busvfulgidus,
`
`Aspergillus clavatus, Athalia rosae, Bacillus acidocaldarius, Bombyx mandarina, Bombyx mori,
`
`Bos taurus, Burkholderia gladioli, Caenorhabditis elegans, Canisfamiliaris, Cavia porcellus,
`
`Chloroflexus aurantiacus, Felis catus, Fervidobacterium nodosum, Helicoverpa armigera,
`
`Homo sapiens, Macacafascicularis, Malus pumila, Mesocricetus auratus, Mus musculus, Musca
`
`7/61
`
`
`
`ldomestica, Mycoplasma hyopneumoniae, Myxococcus xanthus, Neosartoryafischeri,
`
`Oryctolagus cuniculus, Paeonia suffiuticosa, Pseudomonas aeruginosa, Rattus norvegicus,
`
`Rubrobacter xylanophilus, Spodoptera exigua, Spodoptera litura, Sulfolobus acidocaldarius,
`
`Sulfolobus shibatae, Sulfolobus solfataricus, Sus scrofa, Thermotoga maritime, Thermus
`
`thermophilus, Vaccinium corymbosum, Vibrio harveyi, Xenopsylla cheopis, Yarrowia lipolytica,
`
`or a combination thereof.
`
`43. (Withdrawn) The composition of claim 42, wherein the lipolytic enzyme comprises a
`
`thermophilic carboxylesterase derived fiom Aeropyrum pernix, Alicyclobacillus acidocaldarius,
`
`Archaeoglobus fulgidus, Bacillus acidocaldarius, Pseudomonas aeruginosa, Sulfolobus
`
`shibatae, Sulfolobus solfataricus, Thermotoga maritime, or a combination thereof.
`
`44. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a lipase
`
`derived from Acinetobacter, Aedes aegypti, Anguillajaponica, Antrodia cinnamomea,
`
`Arabidopsis rosette, Arabidopsis thaliana, Arxula adeninivorans, Aspergillus niger, Aspergillus
`
`oryzae, Aspergillus tamarii, Aureobasidium pullulans, Avena sativa, Bacillus licheniformis,
`
`Bacillus sphaericus, Bacillus stearothermophilus, Bacillus subtilis, Bacillus thermocatenulatus,
`
`Bacillus thermoleovorans, Bombyx mandarina, Bombyx mori, Bos Taurus, Brassica napus,
`
`Brassica rapa,- Burkholderia cepacia, Caenorhabditis elegans, Candida albicans, Candida
`
`antarctica, Candida deformans, Candida parapsilosis, Candida rugosa, Candida thermophila,
`
`4
`
`Canis domesticus, Chenopodium rubrum, Clostridium beijerinckii, Clostridium botulinum, .
`
`Clostridium novyi, Danio rerio, Galactomyces geotrichum, Gallus gallus, Geobacillus,
`
`Gibberella zeae, Gossypium hirsutum, Homo sapiens, Kurtzmanomyces sp., Leishmania
`
`infantum, Lycopersicon esculentum L, Malasseziafurfur, Methanosarcina acetivorans, Mus
`
`musculus, Mus spretus, Mycobacterium tuberculosis, Mycoplasma hyopneumoniae, Myxococcus
`
`xanthus, Neosartoryafischeri, Oryctolagus cuniculus, Oryza sativa, Penicillium cyclopium,
`
`Phlebotomus papatasi, Pseudomonas aeruginosa, Pseudomonasfluorescens, Pseudomonas
`
`fragi, Pseudomonas sp, Rattus norvegicus, Rhizomucor miehei, Rhizopus oryzae, Rhizopus
`
`stolonifer, Ricinus communis, Samia cynthia ricini, Schizosaccharomyces pombe, Serratia
`
`marcescens, Spermophilus tridecemlineatus, Staphylococcus simulans, Staphylococcus xylosus,
`
`8/6]
`
`
`
`Sulfolobus solfataricus, Sus scrofa, Thermomyces lanuginosus, Trichomonas vaginalis, Vibrio
`
`harveyi, Xenopus laevis, Yarrowia lipolytica, or a combination thereof.
`
`45. (Withdrawn) The composition of claim 44, wherein the lipase comprises a thermophilic
`
`lipase derived from Acinetobacter calcoaceticus, Acinetobacter sp., Bacillus sphaericus,
`
`Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Candida
`
`rugosa, Candida thermophila, GeoBacillus thermoleovorans Toshki, Pseudomonasfragi,
`
`Staphylococcus xylosus, Sulfolobus solfataricus, or a combination thereof.
`
`46. (Withdrawn) The composition of claim 44, wherein the lipase comprises a psychrophilic
`
`lipase derived from Pseudomonasfluorescens.
`
`47. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`lipoprotein lipase derived from Capra hircus, Danio rerio, Felis catus,, Homo sapiens,
`
`Mesocricetus auratus, Mus musculus, Oncorhynchus mykiss, Pagrus major, Papio Anubis,
`
`Rattus norvegicus, Sparus aurata, Sus scrofa, Thunnus-orientalis, or a combination thereof.
`
`48. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises an
`
`acylglycerol lipase derived from Bacillus sp., Danio rerio, Homo sapiens, Leishmania infantum,
`
`Mus musculus, Mycobacterium tuberculosis, Penicillium camembertii, Rattus norvegicus,
`
`Solanum tuberosum, or a combination thereof.
`
`49. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`hormone sensitive lipase derived from Bos Taurus, Homo sapiens, Mus musculus, Rattus
`
`norvegicus, Spermophilus tridecemlineatus, Sus scrofa, Tetrahymena thermophila, or a
`
`combination thereof.
`
`50. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`phospholipase A. derived from Arabidopsis, Aspergillus oryzae, Bos Taurus, Brassica rapa,
`
`Caenorhabditis elegans, Capsicum annuum, Danio rerio, Homo sapiens, Mus musculus,
`
`9/61
`
`
`
`Nicotiana tabacum, Polistes annularis, Polybia paulisla, Rattus norvegicus, Serratia sp.,
`
`Vespula vulgaris, or a combination thereof.
`
`5 i. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`phospholipase A2 derived from Acanthaster planci, Adamsia carciniopado, Aedes aegypti,
`
`Aeropyrum pernix, Aipysurus eydouxii, Apis mellifera, Arabidopsis thaliana, Aspergillus
`
`nidulans, Austrelaps superbus, Bitis gabonica, Bos taurus, Bothriechis schlegelii, Bothrops
`jararacussu, BrachyDanio rerio, Bungarus caeruleus, Bungarusfasciatus, Canisfamiliaris,
`
`Cavia sp., Cerrophidion godmani, Chlamydomonas reinhardtii, Chrysophrys major, Crotalus
`
`viridis viridis, Daboia russellii, Danio rerio, Drosophila melanogaster, Echis carinatus, Echis
`
`ocellatus, Echis pyramidum leakeyi, Emericella nidulans, Equus caballus, Gallus gallus, Homo
`
`sapiens, Lapemis hardwickii, Laticauda semifasciata, Micrurus corallines, Mus musculus,
`
`Mytilus edulis, Naja kaouthia, Naja naja, Naja naja sputatrix, Nicotiana tabacum, Ophiophagus
`
`hannah, Ornithodoros parkeri, Ochtolagus cuniculus, Pagrus major, Patiria pectinifera,
`
`Polyandrocarpa misakiensis, Protobothrops mucrosquamatus, Rattus norvegicus, Sistrurus
`
`catenatus tergeminus, Trimeresurus borneensis, Trimeresurusflavoviridis, Trimeresurus
`
`gracilis, Trimeresurus gramineus, Trimeresurus okinavensis, Trimeresurus puniceus,
`
`Trimeresurus stejnegeri, Tuber borchii, Urticina crassicornis, Vipera russelli siamensis,
`
`Xenopus laevis, Xenopus tropicalis, or a combination thereof.
`
`52. (Withdrawn) The composition of claim 51, wherein the phospholipase A2 comprises a
`
`thermophilic phospholipase A2 derived from Aeropyrum pernix.
`
`53. (Withdrawn) The composition of claim 40, wherein the Iipolytic enzyme comprises a
`
`phospholipase C derived from Aedes aegypti, Aplysia californica, Arabidopsis thaliana, Asterina
`
`miniata, Bacillus cereus, Bacillus thuringiensis, Bos taurus, Caenorhabditis elegans,
`
`Chaetopterus pergamentaceus, Chlamydomonas reinhardtii, Coturnixjaponica, Danio rerio,
`
`Dictyostelium discoideum, Drosophila melanogaster, Gallus gallus, Homarus americanus,
`
`Homo sapiens, Loligo pealei, Lytechirzus pictus, Meleagris gallopavo, Misgurnus mizolepis, Mus
`
`musculus, Nicotiana tabacum, 0ryza sativa, Oryzias latipes, Petunia inflate, Pichia stipitis,
`
`Pisum sativum, Plasmodiumfalciparum, Rattus norvegicus, Strongylocentrotus purpuratus, Sus
`
`10/61
`
`
`
`scrofa, Toreniafimmieri, Toxoplasma gondii, Watasenia scintillans, Xenopus laevis, Zea mays,
`
`or a combination thereof.
`
`54. (Withdrawn) The composition of claim 53, wherein the phospholipase C comprises a
`
`thermophilic phospholipase C derived from Bacillus cereus.
`
`55. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`phospholipase D derived from Aedes aegypti, Arabidopsis thaliana, Arachis hypogaea, Bos
`
`taurus, Brassica oleracea, Caenorhabditis elegans, Cricetulus griseus, Cucumis melo var.
`inodorus, Cucumis sativus, Dictyostelium discoideum, Drosophila melanogaster, Emericella
`
`nidulans, Fragaria ananassa, Gossypium hirsutum, Homo sapiens, Lolium temulentum,
`
`Lycopersicon esculentum, Mus musculus, Oryza sativa, Papaver somm'ferum, Paralichthys
`
`olivaceus, Pichia stipitis, Pimpinella brachycarpa, Rattus norvegicus, Ricinus communis,
`Slreptoverticillium cinnamoneum, Vigna unguiculata, Vitis vinifera, Zea mays, or a combination
`
`thereof.
`
`56. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`phosphoinositide phospholipase C derived from Arabidopsis thaliana, Aspergillus clavatus,
`
`Aspergillusfumigatus, Brassica napus, Homo sapiens, Leishmania infantum, Mus musculus,
`
`Neosartoryafischeri, Physcomitrella patens, Pichia stipitis, Rattus norvegicus, Toxoplasma
`
`gondii, Trypanosoma brucei, Vigna unguiculata, Xenopus tropicalis, Zea mays, or a combination
`
`thereof.
`
`57. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`phosphatidate phosphatase derived 'from Saccharomyces'cerevisiae.
`
`58. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`lysophospholipase derived from Aedes aegypti, Argas monolakensis, Aspergillus clavatus,
`
`Aspergillusfumigatus, Bos Taurus, Cavia porcellus, Clonorchis sinensis, Danio rerio,
`
`Dictyostelium discoideum, Emericella nidulans, Giardia lamblia, Homo sapiens, Monodelphis
`
`domestica, Mus musculus, Neosartoryafischeri, Pichiajadinii, Pichia stipitis, Rattus
`
`11/6]
`
`
`
`norvegicus, Schistosomajaponicum, Schizosaccharomyces pombe, Sclerotinia sclerotiorum,
`
`Xenopus tropicalis, or a combination thereof.
`
`59. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a sterol
`
`esterase derived from Candida rugosa, Homo sapiens, Melanocarpus albomyces, Rattus
`
`norvegicus, or a combination thereof.
`
`60. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`galactolipase derived from Homo sapiens, Solanum tuberosum, Vigna unguiculata, or a
`
`combination thereof.
`
`61. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`sphingomyelin phosphodiesterase derived from Bacillus cereus, Homo sapiens, Pseudomonas
`
`sp., or a combination thereof.
`
`62. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`ceramidase derived from Homo sapiens, Pseudomonas, or a combination thereof.
`
`63. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a retinyl
`
`palmitate esterase derived from Bos Taurus.
`
`64. (Withdrawn) The composition of claim 40, wherein the lipolytic enzyme comprises a
`
`cutinase derived from Fusarium solanipisi, Moniliniafructicola, Pseudomonas putida, or a
`
`combination thereof.
`
`65. (Original) The composition of claim 1, wherein the active enzyme comprises a mesophilic
`
`enzyme, a psychrophilic enzyme, a thermophilic enzyme, a halophilic enzyme, or a combination
`
`thereof.
`
`66. (Original) The composition of claim 1, wherein the coating comprises a stimulator of"
`
`enzyme activity.
`
`12/6]
`
`
`
`67. (Original) The composition of claim 1, wherein the active enzyme comprises an
`
`immobilization carrier.
`
`68. (Previously Presented) The composition of claim 67, wherein the immobilization carrier
`
`comprises a reverse micelle, zeolite, Celite Hyflo Supercel, a resin, diatomaceous earth, a
`
`polyurethane foam particle, macroporous polypropylene Accurel® EP 100, a macroporous
`
`anionic resin bead, polypropylene membrane, acrylic membrane, nylon membrane, cellulose
`
`ester membrane, polyvinylidene difuoride membrane, filter paper, teflon membrane, ceramic
`
`membrane, a macroporous packing particulate, polyamide, cellulose hollow fibre, a
`
`polypropylene membrane pretreated with a blocked copolymer, an immunoglobin, agarose, a gel,
`
`or a combination thereof.
`
`69. (Withdrawn) The composition of claim 1, wherein the active enzyme comprises a purified
`
`active enzyme.
`
`70. (Original) The composition of claim 1, wherein the active enzyme comprises a cell-based
`
`particulate material.
`
`71. (Original) The composition of claim 70, wherein the cell—based particulate material
`
`comprises a microorganism-based particulate material.
`
`72. (Original) The composition of claim 70, wherein the cell-based particulate material is a
`
`whole cell particulate material.
`
`_
`
`73. (Original) The composition of claim 70, wherein the cell-based particulate material is a cell
`
`fragment particulate material.
`
`74. (Withdrawn) The composition of claim 1, wherein the active enzyme is prepared in a
`
`material that is attenuated.
`
`l3/6l
`
`
`
`75. (Original) The composition of claim 1, wherein the active enzyme is prepared in a material
`
`that is sterilized.
`
`76. (Withdrawn) The composition of claim I, wherein the active enzyme comprises a petroleum
`
`lipolytic enzyme.
`
`77. (Original) The composition of claim 1, wherein the active enzyme comprises about 0.1% to
`
`about 80% of the coating by weight or volume.
`
`78. (Original) The composition of claim 1, wherein the active enzyme comprises a particulate
`
`material.
`
`79. (Original) The composition of claim 78, wherein the average wet molecular weight or dry
`
`molecular weight of a primary particle of the particulate material is about 50 kDa to about l.5 x
`
`10'4 kDa.
`
`80. (Previously Presented) The composition of claim 79, wherein the average active enzyme
`
`content per primary particle of the particulate material is about 0.000000]% to about 100%.
`
`81. (Original) The composition of claim l, wherein the coating is about 5 um to about 5000 um
`
`thick upon a surface.
`
`82. (Original) The composition of claim 1, wherein the coating comprises a paint.
`
`83. (Withdrawn) The composition of claim I, wherein the coating comprisesa clear coating.
`
`84. (Withdrawn) The composition of claim 83, wherein the clear coating comprises a lacquer, a
`
`varnish, a shellac, a stain, a water repellent coating, or a combination thereof.
`
`85. (Withdrawn) The composition of claim 1, wherein the coating comprises a multicoat
`
`system.
`
`14/61
`
`
`
`86. (Withdrawn) The composition of claim 85, wherein the multicoat system comprises 2 to 10
`
`layers.
`
`87. (Withdrawn) The composition of claim 85, wherein one layer of the multicoat system
`
`comprises the active enzyme.
`
`88. (Withdrawn) The composition of claim 85, wherein a plurality of layers of the multicoat
`
`system comprise the active enzyme.
`
`89. (Withdrawn) The composition of claim 88, wherein at least one layer of said plurality of
`
`layers comprises a different preparation of the active enzyme than at least a second layer of said
`
`plurality of layers that comprises the active enzyme.
`
`90. (Withdrawn) The composition of claim 85, wherein each layer of the multicoat system is
`
`coating is about 5 um to about 5000 um thick upon a surface.
`
`91. (Withdrawn) The composition of claim 85, wherein the multicoat system comprises a
`
`sealer, a water repellent, a primer, an undercoat, a topcoat, or a combination thereof.
`
`92. (Withdrawn) The composition of claim 85, wherein the multicoat system comprises a
`
`topcoat.
`
`93. (Withdrawn) The composition of claim 92, wherein the topcoat comprises the active
`
`enzyme.
`
`94. (Previously Presented) The composition of claim 1, wherein the coating is capable of film
`
`formation.
`
`95. (Original) The composition of claim 94, wherein film formation occurs between about —
`
`10°C to about 40°C.
`
`15/61
`
`
`
`96. (Withdrawn) The composition ofclaim 94, wherein film formation occurs at baking
`
`conditions.
`
`97. (Original) The composition of claim 94, wherein the coating comprises a volatile
`
`component and a non-volatile component.
`
`98. (Original) The composition of claim 97, wherein the coating undergoes film formation by
`
`loss of part of the volatile component.
`
`99. (Withdrawn) The composition of claim 94, wherein film formation occurs by crosslinking
`
`of a binder.
`
`100. (Withdrawn) The composition of claim 99, wherein film formation occurs by crosslinking
`
`ofa plurality of binders.
`
`101. (Withdrawn) The composition of claim 94, wherein film formation occurs by irradiating
`
`the coating.
`
`102. (Withdrawn) The composition of claim 94, wherein the coating produces a self-cleaning
`
`film.
`
`103. (Withdrawn) The composition of claim 94, wherein the coating produces a temporary film.
`
`104. (Withdrawn) The composition ofclaim 1, wherein the coating is a non-film forming
`
`coating.
`
`105. (Original) The composition of claim 1, wherein the coating comprises architectural
`
`coating.
`
`16/61
`
`
`
`106. (Previously Presented) The composition of claim 105, wherein the architectural coating
`
`comprises an architectural wood coating, an architectural masonry coating, an architectural
`
`artist’s coating, an architectural plastic coating, an architectural metal coating, or a combination
`
`thereof.
`
`107. (Previously Presented) The composition of claim 105, wherein the architectural coating
`
`has a pot life of at least 12 months at about —10°C to about 40°C.
`
`108. (Previously Presented) The composition of claim 105, wherein the architectural coating
`
`undergoes film formation between about —10°C to about 40°C.
`
`109. (Withdrawn, Currently Amended) The composition of claim 1, wherein the coating
`
`comprises an automotive coating, ga can coating,—a—sealant—eeating,—er—a—eembifiatien—thereef.
`
`l 10. (Withdrawn) The composition of claim 109, wherein the coating undergoes film formation
`
`at baking conditions.
`
`1 1 1. (Withdrawn) The composition of claim 1, wherein the coating comprises a chemical agent
`
`resistant coating, a camouflage coating, a traffic marker coating, an aircraft coating, a nuclear
`
`power plant coating, or a combination thereof.
`
`1 12. (Withdrawn) The composition ofclaim 1, wherein the coating comprises a plastic coating.
`
`1 13. (Original) The composition of claim 1, wherein the coating comprises a water-home
`
`coating.
`
`1 14. (Original) The composition of claim 1 13, wherein the water-borne coating is a latex
`
`coating.
`
`1 15. (Original) The composition of claim 1 13, wherein the water-home coating has a density of
`
`about 1.20 kg/L to about 1.50 kg/L.
`
`17/61
`
`
`
`116. (Withdrawn) The composition of claim 1, wherein the coating comprises a solvent-bome
`
`coating.
`
`1 17. (Withdrawn) The composition ofclaim I 16, wherein the solvent-borne coating has a
`
`density of about 0.90 kg/L to about 1.2 kg/L.
`
`I 18. (Original) The composition of claim 1, wherein the coating has a low-shear viscosity of
`
`about 100 P to about 3000 P.
`
`119. (Original) The composition of claim 1, wherein the coating comprises a binder, a liquid
`
`component, a colorant, an additive, or a combination thereof.
`
`120. (Original) The composition of claim 1 19, wherein the coating comprises a binder.
`
`121. (Original) The composition of claim 120, wherein the binder comprises a thermoplastic
`
`binder, a thermosetting binder, or a combination thereof.
`
`122. (Original) The composition of claim 121, wherein the coating comprises a thermoplastic
`
`binder.
`
`123. (Previously Presented) The composition of claim 122, wherein the coating is capable of
`
`producing a film by thermoplastic film formation.
`
`124. (Withdrawn) The composition of claim 121, wherein the coating comprises a
`
`thermosetting binder.
`
`125. (Withdrawn) The composition of claim 124, wherein the coating is capable of producing a
`
`film by thermosetting film formation.
`
`126. (Original) The composition of claim 1 19, wherein the coating comprises a liquid
`
`component.
`
`18/61
`
`
`
`127. (Original) The composition of claim 126, wherein the liquid component comprises a
`
`solvent, a thinner, a diluent, a plasticizer, or a combination thereof.
`
`128. (Original) The composition of claim 126, wherein the liquid component comprises a liquid
`
`organic compound, an inorganic compound, water, or a combination thereof.
`
`129. (Previously Presented) The composition of claim 119, wherein the coating comprises a
`
`colorant.
`
`130. (Original) The composition of claim 129, wherein the colorant comprises a pigment, a dye,
`
`or a combination thereof.
`
`131. (Withdrawn) The composition of claim 1, wherein the coating comprises a metal surface
`
`coating.
`
`132. (Previously Presented) The composition of claim 1 19, wherein the coating comprises an
`
`additive.
`
`133. (Original) The composition of claim 132, wherein the additive comprises 0.000001% to
`
`20.0% by weight, of the coating.
`
`134. (Original) The composition of claim, 133, wherein said additive comprises an accelerator,
`
`an adhesion promoter, an antifoamer, anti-insect additive, an antioxidant, an antiskinning agent,
`
`a buffer, a catalyst, a coalescing agent, a corrosion inhibitor, a defoamer, a dehydrator, a
`
`dispersant, a drier, electrical additive, an emulsifier, a filler, a flame/fire retardant, a flatting
`
`agent, a flow control agent, a gloss aid, a leveling agent, a marproofing agent, a preservative, a
`
`silicone additive, a slip agent, a surfactant, a light stabilizer, a rheological control agent, a
`
`wetting additive, a cryopreservative, a xeroprotectant, or a combination thereof.
`
`135. (Original) The composition ofclaim 134, wherein the additive comprises a preservative.
`
`19/61
`
`
`
`136. (Original) The composition of claim 135, wherein the preservative comprises an in-can
`
`preservative, an in-film preservative, or a combination thereof.
`
`137. (Original) The composition of claim 135, wherein the preservative comprises a biocide, a
`
`biostatic, or a combination thereof.
`
`138. (Original) The composition of claim 137, wherein the biocide comprises a bactericide, a
`
`fungicide, an algaecide, or a combination thereof.
`
`139. (Original) The composition of claim 135, wherein the preservative comprises l-(3-
`
`ch|oroallyl)-3,5,7-triaza-l-azoniaadamantane chloride; 1,2-benzisothiazoline-3-one; 1,2-
`
`dibromo-2,4-dicyanobutane; l,3-bis(hydroxymethyl)-5,5-dimethylhydantoin; l-methyl—3,5,7-
`
`triaza-l-azonia-adamantane chloride; 2-bromo-2-nitropropane-l,3-diol; 2-(4-
`
`thiazolyl)benzimidazole; 2-(hydroxymethyl)-amino-2-methyl-l-pr0panol; 2(hydroxymethyl)-
`
`aminoethanol; 2,2—dibromo-3-nitrilopropionamide; 2,4,5,6—tetrachloro—isophthalonitrile; 2-
`
`mercaptobenzo-thiazole; 2-methyl-4-isothiazolin-3—one; 2-n-octyl-4-isothiazoline-3-one; 3-iodo-
`
`2-propynl N-butyl carbamate; 4,5—dichloro-2-N-0ctyl-3(2H)-isothiazolone; 4,4-
`
`dimethyloxazolidine; 5-chloro-Z-methyl~4-isothiazolin-3-one; 5-hydroxy-methyl-l-aza-3,7-
`
`dioxabicylco (3.3.0.) octane; 6-acetoxy-2,4-dimethyl-l ,3-dioxane; 7-ethyl bicyclooxazolidine; a
`
`combination of l,2-benzisothiazoline-3-one and hexahydro-l,3,5-tris(2-hydroxyethyl)-s-triazine;
`
`a combination of l,2-benzisothiazoline-3-one and zinc pyrithione; a combination of 2-
`
`(thiocyanomethyl—thio)benzothiozole and methylene bis(thiocyanate); a combination of 4-(2-
`
`nitrobutyl)-morpholine and 4,4’-(2-ethy|nitrotrimethylene) dimorpholine; a combination of 4,4-
`
`dimethyl-oxazolidine and 3,4,4-trimethyloxazolidine; a combination of 5-chloro-2-methyl-4-
`isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one; acombination of carbendazim and 3-iodo-
`2-propynl N-butyl carbamate; a combination of carbendazim, 3-iodo-2-propynl N-butyl
`carbamate and diuron; a combination of chlorothalonil and 3-iodo-2-propynl N—butyl carbamate;
`
`a combination of chlorothalonil and a triazine compound; a combination of tributyltin benzoate
`and alkylamine hydrochlorides; a combination of zinc-dimethyldithiocarbamate and zinc 2-
`
`mercaptobenzothiazole; a copper soap; a metal soap; a mercury soap; a mixture of bicyclic
`
`oxazolidines; a tin soap; an alkylamine hydrochloride; an amine reaction product; barium
`
`20/61
`
`
`
`metaborate; butyl parahydroxybenzoate; carbendazim; copper(11) 8-quinolinolate; diiodomethyl-
`
`p-tolysulfone; dithio-2,2-bis(benzmethylamide); diuron; ethyl parahydroxybenzoate;
`
`glutaraldehyde; hexahydro-l ,3,5-triethyl-s-triazine; hexahydro- 1 ,3,5-tris(2-hydroxyethyl)-s-
`
`triazine; hydroxymethyl-S,5-dimethylhydantoin; methyl parahydroxybenzoate; N-butyl—1,2-
`
`I
`
`benzisothiazolin-3-one; N-(trichloromethylthio) phthalimide; N-cyclopropyl-N-(l-
`
`dimethylethyl)-6—(methylthio)-1,3,5-triazine-2,4-diamine; N-trichloromethyl-thio-4-
`
`cyclohexene-l ,2-dicarboximide; p-chloro-m-cresol; phenoxyethanol; phenylmercuric acetate;
`
`poly(hexamethylene biguanide) hydrochloride; potassium dimethyldithiocarbamate; potassium
`
`N-hydroxy-methyl—N-methyl-dithiocarbamate; propyl parahydroxybenzoate; sodium 2—
`
`pyridinethiol-l -oxide; tetra-hydro-3,5-di-methyl-2H-1,3,5-thiadia2ine-2-thlone; tributyltin
`
`benzoate; tributyltin oxide; tributyltin salicylate; zinc pyrithione; sodium pyrithione; copper
`
`.
`
`pyrithione; zinc oxide; a zinc soap; or a combination thereof.
`
`140. (Withdrawn) The composition of claim 1, wherein the coating is a multi-pack coating.
`
`141. (Withdrawn) The composition of claim 140, wherein the multi-pack coating is stored in a
`
`two to five containers prior to application to a surface.
`
`142. (Withdrawn) The composition of claim 140, wherein about 0.000001% to about 100% of
`
`the active enzyme is stored in a container of the multi-pack coating, and at least one coating
`
`component is stored in another container of the multi—pack coating.
`
`143. (Original) The composition ofclaim 1, wherein the coating is a coating capable of being
`
`applied to a surface by a spray applicator.
`
`144. (Withdrawn) The composition of claim 1, wherein the active enzyme is
`
`microencapsulated.
`
`145. (Withdrawn) The composition of claim 1, wherein the coating comprises a pH indicator.
`
`21/61
`
`
`
`146. (Withdrawn) The composition of claim 1, wherein the esterase comprises a phosphoric
`
`triester hydrolase.
`
`147. (Withdrawn) The composition of claim 1, wherein the esterase comprises a plurality of
`
`phosphoric triester hydrolases.
`
`148. (Withdrawn) The composition of claim 1, wherein the active enzyme comprises an
`
`6816 rase.
`
`149. (Withdrawn) The composition of claim 1, wherein the active enzyme comprises a plurality
`
`of esterases.
`
`150. (Withdrawn) The composition of claim 1, wherein the active enzyme comprises a
`
`peptidase.
`
`151. (Withdr
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