Appln. N0.: 11/388,723
`Reply to Restriction Requirement of June 5, 2007
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`AMENDMENT
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`The following listing of claims replaces all prior listings of claims in this application.
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`1.
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`'
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`(Original) An apparatus for detecting an analyte in a biological fluid of a subject,
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`comprising:
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`a) a sample collection unit for introducing a biological fluid in fluid communication with a
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`plurality of reaction sites;
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`b) a plurality of reactant chambers carrying a plurality of reactants in fluid communication
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`with said reaction sites,
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`wherein said plurality of reaction sites comprise a plurality of reactants boundlthereto for
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`detecting said analyte; and
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`c) a system of fluidic channels to allow said biological fluid and said plurality of reactants to
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`flow in said apparatus, wherein at least one channel located between said plurality of reaction
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`sites comprises an optical barrier to reduce the amount of optical cross—talk between said
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`plurality of said reaction sites during detection of said analyte.
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`2.
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`(Original) The apparatus of claim 1, further comprising a plurality of waste chambers in
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`fluid communication with at least one of said reaction sites.
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`3.
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`(Original) The apparatus of claim 1 wherein each channel located between said plurality of
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`reaction sites comprises an optical barrier.
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`4.
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`(Original) The apparatus of claim 1 wherein the biological fluid is less than about 500
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`microliters.
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`5.
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`(Original) The apparatus of claim 1 wherein said optical barrier comprises a nonlinear
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`fluidic channel.
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`3134264_1.DOC
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`Atty. Docket No. 30696-712201
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`
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`Appln. No.: 11/388,723
`Reply to Restriction Requirement of June 5, 2007
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`6.
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`7.
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`8.
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`(Original) The apparatus of claim 1 wherein the fluid is less than about 50 microliters.
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`(Original) The apparatus of claim 1, wherein the reactants comprise immunoassay reagents.
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`(Original) The apparatus of claim 7, wherein said apparatus detects a plurality of analytes,
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`wherein the analytes are identified by distinct signals detectable over a range of 3 orders of
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`magnitude.
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`9.
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`(Original) The apparatus of claim 7, wherein the immunoassay reagents detect a polypeptide
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`glycoprotein, polysaccharide, lipid, nucleic acid, and a combination thereof.
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`10.
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`(Original) The apparatus of claim 7, wherein the immunoassay reagents detect a member
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`selected from the group consisting of drug, drug metabolite, biomarker indicative of a disease, tissue
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`specific marker, and biomarker specific for a cell or cell type.
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`11.
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`(Original) The apparatus of claim 1, wherein the detectable signal is a luminescent signal.
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`12.
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`(Original) An apparatus for detecting an analyte in a biological fluid of a subject, comprising
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`a) a sample collection unit for introducing a biological fluid in fluid communication with a
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`plurality of reaction sites, wherein said plurality of reaction sites comprise a plurality of
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`bound reactants for detecting said analyte;
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`b) a plurality of reactant chambers carrying a plurality of reactants in fluid communication
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`with said reaction sites; and
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`c) a system of fluidic channels to allow said biological fluid and said plurality of reactants to
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`flow in said apparatus,
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`wherein said bound reactants in at least one reaction site are unevenly distributed.
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`13.
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`(Original) The apparatus of claim 12, wherein said bound reactants in the at least one
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`reaction site are localized around the center of said reaction site.
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`3134264_1.DOC
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`Atty. Docket No. 30696-712201
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`-3-
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`Appln. No.: 11/388,723
`Reply to Restriction Requirement of June 5, 2007
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`14.
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`(Original) The apparatus of claim 13, wherein an outer edge of the at least one reaction site
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`is at a distance sufficiently far from said bound reactants to reduce signals unrelated to the presence
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`of said analyte.
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`15.
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`(Original) The apparatus of claim 12, further comprising a waste chamber in fluid
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`communication with said reaction sites.
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`16.
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`(Original) The apparatus of claim 14 wherein said unbound reactant is coupled to said edge
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`of said reaction site.
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`17.
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`(Withdrawn) A method of manufacturing a fluidic device for detecting an analyte in a
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`biological fluid of a subject, comprising:
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`a) providing a plurality of layers of a fluidic device;
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`b) ultrasonically welding said layers together such that a fluidic network exists between a
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`sample collection unit, at least one reactant chamber, at least one reaction site, and at least
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`one waste chamber.
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`18. (Withdrawn) The method of claim 17 wherein said at least one reaction chamber is vacuum
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`sealed.
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`19. (Withdrawn) The method of claim 17, wherein said fluidic device comprises a system of fluidic
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`channels to allow contact of said biological fluid with reactants in said at least one reaction site.
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`20. (Withdrawn) The method of claim 17, wherein said reaction site comprises an optical barrier to
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`reduce the amount of optical cross-talk between another reaction site on the fluidic device.
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`3134264_1.DOC
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`Atty. Docket No. 30696-712201
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