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`molecular beacons
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`Introduction on Molecular Beacons
`
`Molecular beacons are single-stranded oligonucleotide hybridization probes that form a stem-and-loop structure.
`The loop contains a probe sequence that is complementary to a target sequence, and the stem is formed by the
`annealing of complementary arm sequences that are located on either side of the probe sequence. A fluorophore
`is covalently linked to the end of one arm and a quencher is covalently linked to the end of the other arm.
`Molecular beacons do not fluoresce when they are free in solution. However, when they hybridize to a nucleic
`acid strand containing a target sequence they undergo a conformational change that enables them to fluoresce
`brightly.
`
`In the absence of targets, the probe is dark, because the stem places the fluorophore so close to the
`nonfluorescent quencher that they transiently share electrons, eliminating the ability of the fluorophore to
`fluoresce. When the probe encounters a target molecule, it forms a probe-target hybrid that is longer and more
`stable than the stem hybrid. The rigidity and length of the probe-target hybrid precludes the simultaneous
`existence of the stem hybrid. Consequently, the molecular beacon undergoes a spontaneous conformational
`reorganization that forces the stem hybrid to dissociate and the fluorophore and the quencher to move away
`from each other, restoring fluorescence.
`
`click here to read more on molecular beacons
`
`July 10, 2024
`
` search
` www.molecular-beacons.org
`
` www
`
`Recent Publications from our group
`
`Ma MT, Jiang Q, Chen CH, Badeti S, Wang X, Zeng
`C, Evans D, Bodnar B, Marras SAE, Tyagi S,
`Bharaj P, Yehia G, Romanienko P, Hu W, Liu SL,
`Shi L, and Liu D (2024) S309-CAR-NK cells bind
`the Omicron variants in vitro and reduce SARS-
`CoV-2 viral loads in humanized ACE2-NSG
`mice. Journal of Virology: e0003824. PMID:
`38767356: PubMed Link
`
`Banada PP, Green R, Streck D, Kurathi R, Reiss R,
`Banik S, Montalvan I, Jones R, Marras SAE,
`Chakravorty S, and Alland D (2023) An expanded
`RT-PCR melting temperature coding assay to
`rapidly identify all known SARS-CoV-2 variants
`and sub-variants of concern. Scientific Reports
`13. 21927. PMID: 38081834: PubMed Link
`
`Ebraham L, Xu C, Wang A, Hernandez C, Siclari N,
`Rajah D, Walter L, Marras SAE, Tyagi S, Fine DH,
`Daep CA, and Chang TL (2023) Oral Epithelial
`cells expressing low or undetectable levels of
`human angiotensin-converting enzyme 2 are
`susceptible to SARS-CoV-2 virus infection in
`vitro. Pathogens 12. PMID: 37375533: PubMed
`Link
`
`Public Health Research Institute
`New Jersey Medical School - Rutgers, The State University of New Jersey
`© 2024 All rights reserved.
`
`https://molecular-beacons.org/MB_introduction.html
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`BIO-RAD EX.1037.001
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