Paul Anthony Dalby, FRSC, AMIChemE
`Dept. Biochemical Engineering, University College London, Gower Street, London WC1E 6BT
`
`EDUCATION AND CAREER
`• 2016 - present Deputy Head, Department of Biochemical Engineering (Research), UCL.
`• 2016 - present Co-Director of EPSRC Future Targeted Healthcare Manufacturing Hub, UCL.
`• 2013 - present Professor at University College London, Dept. Biochemical Engineering
`Director of EPSRC Centre for Doctoral Training in Innovative Manufacturing, UCL.
`• 2012 - 2022
`Co-Director of EPSRC Centre for Innovative Manufacturing, UCL.
`• 2011 - 2016
`Reader at University College London, Dept. Biochemical Engineering
`• 2009 - 2013
`Senior Lecturer at University College London, Dept. Biochemical Engineering
`• 2007 - 2009
`Lecturer at University College London, Dept. Biochemical Engineering
`• 2000 - 2007
`Postdoctoral fellowship at University of Pennsylvania, School of Medicine
`• 1998 - 2000
`
`Protein design and evolution with Prof. W. F. DeGrado (member of the National Academy)
`
`Projects: Development of novel binding proteins
`
`
`
`In vitro evolution of new catalytic function
`
`Collaboration with Ronald H. Hoess (Dupont)
`
`Carried out phage display, protein engineering, purification, formulation and
`
`characterisation by CD, fluorescence, enzymology, computer modeling and bioinformatics
`Ph.D. Cambridge University, MRC Centre for Protein Engineering
`• 1994 - 1998
`
`Protein folding and engineering with Prof. Sir A. R. Fersht, FRS
`
`Projects: Folding pathway of Barnase
`
`My work involved molecular biology, PCR, mutagenesis, expression and purification,
`
` organic synthesis, circular-dichroism, fluorescence and UV-Visible spectroscopy,
`
`
`calorimetry, stopped-flow kinetics, temperature-jump, pulsed quench-flow and NMR.
`B.A. (Hons)/ M.A. Natural Sciences (First class)
`• 1991 - 1994
`
`Gonville & Caius College, Cambridge University
`
`Studied Chemistry, Biochemistry, Physics, and Mathematics
`
`Project: Synthesis of short antimicrobial peptides (organic chemistry)
`
`AWARDS
`• 2010
`• 2010
`• 2008-2010
`• 2001
`• 1994-1997
`• 1993-1994
`• 1993
`
`PROFESSIONAL AFFILIATIONS
`Scientific Advisory Board for UMabs, USA/China.
`• 2022-
`EPSRC Strategic Advisory Team for Manufacturing the Future.
`• 2020-
`EPSRC Healthcare Council-SAN-SAT Working Group.
`• 2020-
`Scientific Advisory Board for Leukocare, Germany.
`• 2020-
`Co-Founder of Roxijen Ltd.
`• 2020-
`UKRI FLF Peer Review College Member
`• 2018
`External examiner MSc Biological Engineering, University of Sheffield
`• 2017- 2020
`EPSRC College member
`• 2016-
`Elected Management Board Member of BBSRC BioProNet
`• 2015-
`Editorial Board Member of Scientific Reports (a Nature Journal)
`• 2015-
`Committee Member of the RSC Chemistry Biology Interface Division
`• 2014-2016
`
`IChemE Innovation and Excellence Award in Bioprocessing (for the BiCE team)
`Royal Society of Chemistry Rita and John Cornforth Award (for the BiCE team)
`Evonik European Science-to-Business Award for Industrial Biotechnology
`Second prize for young researcher at SCI: London's Chemistry meeting
`Gonville & Caius College Dunlop Scholarship
`Gonville & Caius College Senior Scholarship
`James Arthur Ramsay Prize in Chemistry & Biology
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`Panel Member of the Innovate UK IB Catalyst.
`Fellow of the Royal Society of Chemistry (Member since 2005)
`Steering Group Member of the BBSRC Bioprocess Research Industry Club (BRIC)
`Editor-in-Chief of 'Current Biotechnology', since its launch (issue 1: Feb 2012)
`Editor-in-Chief of the 'Open Biotechnology Journal' (Editorial board member since 2007)
`BBSRC Research Grant Panel Committee D as core member
`BBSRC Research Grant Panel Committee C as pool member
`Chair Royal Society of Chemistry - Biotechnology Group (Member since 2003)
`Editorial Board Member of ‘Enzyme Engineering'
`Editorial Advisory Board for 'Recent Patents in Biotechnology'
`Member of the Protein Society
`Associate Member of the Institute of Chemical Engineers
`Member of the American Chemical Society
`
`• 2014-2016
`• 2013-
`• 2013- 2016
`• 2011- 2014
`• 2010- 2014
`• 2011- 2014
`• 2010- 2011
`• 2008-
`• 2011- 2013
`• 2006- 2008
`• 2006- 2013
`• 2003-
`• 2001- 2005
`
`FACULTY, UCL, MULTI-INSTITUTIONAL ROLES
`• 2017 - 2021
`Faculty REF Impact lead
`• 2017 - 2021
`Dept Biochemical Engineering REF lead
`• 2016 -
`Chair of the UCL SLMS Technology Innovation Network for Biologics
`• 2016 -
`Member of Faculty of Engineering Sciences Research Directors Committee
`• 2015 - 2022
`Member of UCL CDT Directors Committee
`• 2012 - 2022
`Director of EPSRC Multi-Institutional Centre for Doctoral Training
`• 2008 -
`Genetic Modification Safety Committee
`• 2016 - 2017
`Member of Faculty of Engineering Sciences Research Degrees Committee
`• 2012 - 2014
`Chair of BBSRC Multi-Institutional Doctoral Training Programme Recruitment Panel
`• 2012 - 2014
`BBSRC Multi-Institutional Doctoral Training Programme Management Board
`• 2005 - 2008
`Engineering Faculty Undergraduate Degree Committee
`
`TEACHING ROLES
`Course coordinator MBI® in Antibody Targeted Therapy
`• 2017 -
`Course coordinator (Yr3), BENG0031. Chemistry for Biochemical Engineers
`• 2016 -
`• 2014 -
`BENG101P. Lectures (formulation, vaccines) Introduction to Biochemical Engineering
`Course coordinator (Yr2), BENG0013. Biochemistry for Biochemical Engineers
`• 2013 -
`• 2007 -
`MBI® in Design of Experiments for Bioprocess Optimisation. Case study lecture.
`• 2000 -
`G004/M002 Advanced Bioreactor Engineering - Lectures (MSc & Yr4)
`• 2000 -
`Research project supervision (Yr4, MSc, MRes & Biotechnology BSc)
`• 2012 - 2018
`BBSRC LIDO Doctoral Training Programme. Lecture- Biological design and engineering.
`Course coordinator (Yr 4), BENGM005/6, 3010 Undergraduate research projects
`• 2003 - 2017
`Course coordinator and lecturer, MBI® in biocatalysis
`• 2005 - 2017
`• 2001 - 2014
`BENG2006 Biocatalyst design lectures (Yr3 Biotechnology)
`Course coordinator (Yr1), BENG1001 Introduction to Biochemical Engineering
`• 2005 - 2014
`• 2000 - 2013
`G17 & BIOC2007 Biochemistry for Biochemical Engineers - Workshops (MSc & Yr2)
`• 2006 - 2009
`BIOC2008 Biochemistry for Biochemical Engineers - Workshops (Yr3)
`• 2003 - 2008
`BIOC1001 Biochemistry for Engineers - Industrial biocatalysis lectures (Yr1)
`• 2000 - 2008
`PhD/EngD training day coordinator
`• 2002 - 2004
`E129/E105/G23 - Affinity Chromatography for undergraduate and MSc
`Biochemical Engineering in the 21st Century - Coordinator (Yr1)
`• 2001 - 2005
`• 2000 - 2005
`MBI® in biocatalysis - Enzyme design lecture
`
`TUTORING ROLES AT DEPT BIOCHEMICAL ENGINEERING, UCL
`Chair of Departmental Research Committee
`• 2016 -
`Member of Departmental Finance Committee
`• 2016 -
`Departmental Tutor for Admissions to Doctoral Research
`• 2008 - 2016
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`Mentor to Darren Nesbeth, a new probationary lecturer in the Department
`Mentor to Martina Micheletti, a new probationary lecturer in the Department
`Chair of undergraduate departmental staff student consultative committee
`Crook (UG) and Beaker (PG) Society Liaison
`Departmental Tutor to Undergraduates
`Tutor to 1st year undergraduates
` Organised BE in 21st century seminars to help students see broader picture.
`Incorporated BE in 21st century seminars into E100 course to make examinable.
`
`Undergraduate Admissions tutor
` Developed recruiting strategies, including Video/CD, mailshot via UCAS
`
`Increased total number of applications from 80 to 160 per year
`Convenor of undergraduate departmental staff student consultative committee
`Undergraduate recruitment coordinator
`
`• 2010 - 2012
`• 2007 - 2009
`• 2005 - 2014
`• 2005 - 2008
`• 2005 - 2008
`• 2000 - 2007
`
`
`• 2000 - 2005
`
`
`• 2003 - 2005
`• 2002 - 2005
`
`PUBLICATIONS: H-index 30 (WoK), 35 (Google Scholar)
`
`Published (114) including 4 book chapters, and one book
`
`1. Zhang C, Berg A, Joe CCD, Dalby PA, Douglas AD (2023) Lyophilization to enable distribution of simian
`adenovirus-vectored vaccines without refrigeration. Nature Vaccines. in press.
`
`
`2. Zhang, H., Dalby, P.A. (2022) Stability convergence in antibody coformulations. Molecular Pharmaceutics
`19 (11) 4098-4110.
`
`
`3. Lee, W., Pradhan, S., Zhang, C., Venanzi, N., Li, W., Goldrick, S., Dalby, P.A. (2022) Directed evolution
`for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli. Scientific Reports
`
`
`4. Lee, W., Pradhan, S., Zhang, C., Venanzi, N., Li, W., Goldrick, S., Dalby, P.A. (2022) Directed evolution
`for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli. Research Square
`(pre-print): doi.org/10.21203/rs.3.rs-1522473/v1
`
`
`5. Xiaochen Lyu, Qichao Zhao, Julia Hui, Tiffany Wang, Mengyi Lin, Keying Wang, Jialing Zhang,
`Jiaqian Shentu, Paul A Dalby, Hongyu Zhang, Bo Liu (2022). The Global Landscape of Approved
`Antibody Therapies. Antibody Therapeutics 5(4) 233-257.
`
`
`6. Li Y, Dalby PA (2022) Engineering of enzymes using non-natural amino acids. Bioscience Reports 42(8)
`BSR20220168.
`
`
`7. Kellerman MAW, Almeida T, Rudd TR, Matejtschuk P, Dalby PA (2022) NMR Reveals Functionally
`Relevant Thermally-induced Structural Changes within the Native Ensemble of G-CSF. Molecular
`Pharmaceutics 19(9) 3242-3255.
`
`
`8. Lalaurie CJ, Splevins A, Barata TS, Bunting KA, Higazi DR, Zloh M, Spiteri VA, Perkins SJ, Dalby PA
`(2022) Elucidation of critical pH-dependent structural changes in Botulinum Neurotoxin E. Journal of
`Structural Biology 214(3) 107876.
`
`
`9. Yu HR, Ma S, Li YW, Dalby PA (2022) Hotspots making directed evolution easier. Biotechnol. Adv. 56,
`107926.
`
`
`10. Wood VE, Groves K, Wong LM, Kong L, Bird C, Wadhwa M, Quaglia M, Matejtschuk P, Dalby PA
`(2022) Protein engineering and HDX identifies structural regions of G-CSF critical to its stability and
`aggregation. Molecular Pharmaceutics 19(2), 616-629.
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`11. Phattaraporn Morris, Ribia García-Arrazola, Leonardo Rios-Solis, Paul A. Dalby. (2021) Biophysical
`characterization of the inactivation of E. coli transketolase by aqueous co-solvents. Scientific Reports.
`11(1), 23584.
`
`
`12. Vaud, S, Pearcy, N, Hanzevacki, M, Van Hagen, AMW, Abdelrazig, S, Safo, L, Ehsaan, M, Jonczyk, M,
`Millat, T, Craig, S, Spence, E, Fothergill, J, Bommareddy, RR, Colin, PY, Twycross, J, Dalby, PA,
`Minton, NP, Jager, CM, Kim, DH, Yu, JP, Maness, PC, Lynch, S, Eckert, CA, Conradie, A (Conradie,
`Alex) 2 Bryan, SJ (Bryan, Samantha J (2021) Engineering improved ethylene production: Leveraging
`systems biology and adaptive laboratory evolution. Metabolic Engineering. 67, 308-320.
`
`
`13. Groves, Kate; Ashcroft, Alison; Cryar, Adam; Sula, Altin; Wallace, Bonnie Ann; Stocks, Bradley; Burns,
`Christopher; Cooper-Shepherd, Dale; De Lorenzi, Ersilia; Rodriguez, Elizabeth ; Zhang, Hongyu; Ault,
`James; Ferguson, Jackie; Phillips, Jonathan; Pacholarz, Kamila; Thalassinos, Konstantinos; Luckau, Luise;
`Ashton, Lorna; Durrant, Oliver; Barran, Perdita; Dalby, Paul; Vicedo, Pau; Colombo, Raffaella; Davis,
`Rachel; Parakra, Rinky; Upton, Rosie; Hill, Sarah; Wood, Victoria; Soloviev, Zoja; Quaglia, Milena (2021)
`A reference protocol to assess analytical performance of higher order structural analysis measurements:
`results from an inter-laboratory comparison. Analytical Chem. 93 (26), 9041-9048.
`doi.org/10.1021/acs.analchem.0c04625
`
`
`14. Zhang H, Yang Y, Zhang C, Farid SS, Dalby PA (2021) Machine learning reveals hidden stability code in
`protein native fluorescence. Computational and Structural Biotechnology Journal. 19, 2750-2760.
`
`15. Cheng Zhang, Nuria Codina, Jiazhi Tang, Haoran Yu, Nesrine Chakroun, Frank Kozielski, Paul A. Dalby.
`(2021) Comparison of the pH- and thermally-induced fluctuations of a therapeutic antibody Fab fragment
`by molecular dynamics simulation. Computational and Structural Biotechnology Journal. 19, 2726-2741.
`
`16. Spiteri V, Doutch J, Rambo RP, Gor J, Dalby PA, Perkins SJ (2021) Solution structure of deglycosylated
`human IgG1 shows the role of CH2 glycans in its conformation. Biophysical Journal 200, 1814-1834.
`doi.org/10.1016/j.bpj.2021.02.038
`
`17. Hales JE, Aoudjane S, Aeppli G, Dalby PA (2021). Proof-of-concept analytical instrument for label-free
`optical deconvolution of protein species in a mixture. Journal of Chromatography A 1641, 461968.
`
`18. Colin PY, Dalby PA (2020) Functional and computational identification of a rescue mutation near the
`active site of an mRNA methyltransferase. Scientific Reports 10, (1) 21841
`
`19. Zhang H, Dalby PA (2020) Stability enhancement in a mAB:Fab protein coformulation. Scientific Reports
`10, 1-11. Doi.org/10.1038/s41598-020-77989-w
`
`20. Wood VE, Groves K, Cryar A, Quaglia M, Matejtschuk P, Dalby PA (2020) HDX and in-silico docking
`reveal that excipients stabilise G-CSF via a combination of preferential exclusion and specific hotspot
`interactions. Molecular Pharmaceutics 17 (12) 4637-4651. doi.org/10.1021/acs.molpharmaceut.0c00877
`
`21. Chauhan VM, Zhang H, Dalby PA, Aylott JW (2020) Advancements in the co-formulation of biologic
`therapeutics. J Controlled Release 327, 397-405.
`
`22. Wilkinson HC, Dalby PA (2020) Fine-tuning the activity and stability of an evolved enzyme active-site
`through non-canonical amino-acids. FEBS J 288, 1935-1955. DOI: 10.1111/febs.15560
`
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`23. Yu H, Dalby PA (2020) A beginner’s guide to molecular dynamics simulations and the identification of
`cross-correlation networks for enzyme engineering. Methods Enzymology. 643, 15-49.
`
`24. Wilkinson HC, Dalby PA (2020) The Two-Species Model of transketolase explains donor substrate-
`binding, inhibition and heat-activation. Scientific Reports 10, 4148.
`
`25. Yu H, Hernandez-Lopez RI, Steadman D, Mendez-Sanchez D, Higson S, Cazares-Korner A, Sheppard TD,
`Ward JM, Hailes HC, Dalby PA (2020) Engineering transketolase to accept both unnatural donor and
`acceptor substrates and produce α-hydroxyketones. Febs J. 287, 1758-1776. DOI: 10.1111/febs.15108
`
`26. Wilkinson HC, Dalby PA (2019) Novel insights into transketolase activation by cofactor binding identifies
`two native species subpopulations. Scientific Reports 9, 16116.
`
`27. Hales JE, Matmon G, Dalby PA, Ward JM, Aeppli G. (2019) Virus lasers for biological detection. Nature
`Communications 10:3594. DOI: 10.1038/s41467-019-11604-z
`
`28. Nuria Codina, Cheng Zhang, Nesrine Chakroun, and Paul A. Dalby. (2019) Insights into the stability of a
`therapeutic antibody Fab fragment by molecular dynamics and its stabilization by computational design.
`BioArXiv.
`
`29. Nuria Codina, David Hilton, Cheng Zhang, Nesrine Chakroun, Shahina S Ahmad, Stephen J Perkins, Paul
`A Dalby. (2019) An expanded conformation of an antibody Fab region by X-ray scattering, molecular
`dynamics and smFRET identifies an aggregation mechanism. Journal of Molecular Biology 431(7):1409-
`1425. doi: 10.1016/j.jmb.2019.02.009
`
`30. Mathew J. Robinson, Paul Matejtschuk, Colin Longstaff, Paul A. Dalby. (2019) Selective stabilisation and
`destabilisation of protein domains in tissue-type plasminogen activator using formulation excipients.
`Molecular Pharmaceutics. 16(2): 744-755. DOI: 10.1021/acs.molpharmaceut.8b01024
`
`31. Yu, H, Dalby, PA. (2018) Exploiting correlated molecular-dynamics networks to counteract enzyme
`activity-stability trade-off. Proc. Natl. Acad. Sci. USA 115 (52) E12192-E12200.
`
`32. Yu, H, Dalby, PA. (2018) Coupled molecular dynamics mediate long and short-range epistasis between
`mutations that affect stability and aggregation kinetics. Proc. Natl. Acad. Sci. USA 115 (47) E11043-
`E11052.
`
`33. Pandya A, Howard MJ, Zloh M, Dalby PA. (2018) An evaluation of the potential of NMR spectroscopy
`and computational modelling methods to inform biopharmaceutical formulations. Pharmaceutics 10 (4),
`165. DOI: 10.3390/pharmaceutics10040165
`
`34. Zhang C, Samad M, Yu H, Chakroun N, Hilton D, Dalby PA. (2018) Computational-design to reduce
`conformational flexibility and aggregation rates of an antibody Fab fragment. Molecular Pharmaceutics.
`15 (8), 3079-3092. DOI: 10.1021/acs.molpharmaceut.8b00186
`
`35. Robinson MJ, Matejtschuk P, Bristow AF, Dalby PA. (2018) Tm-values and unfolded fraction can predict
`aggregation rates for GCSF variant formulations, but not under predominantly native conditions. Molecular
`Pharmaceutics. 15 (1), 256-267. DOI: 10.1021/acs.molpharmaceut.7b00876
`
`36. Cardenas-Fernandez M, Bawn M, Hamley-Bennett C, Bharat PKV, Subrizi F, Suhaili N, Ward D, Bourdin
`S, Dalby PA, Hailes HC, Hewitson P, Ignatova S, Kontoravdi C, Leak D, Shah N, Sheppard TD, Ward JM,
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`Lye GJ. (2017) An integrated biorefinery concept for conversion of sugar beet pulp into value-added
`chemicals and pharmaceutical intermediates. Faraday Discussions, 202, 415-431.
`
`37. Haoran Yu, Yihan Yan, Cheng Zhang, Paul A. Dalby (2017) Two strategies to engineer flexible loops for
`improved enzyme thermostability. Scientific Reports, 7, 41212.
`
`
`38. Affaticati PE, Dai SB, Payongsri D, Hailes HC, Tittmann K, Dalby PA. (2016) Structural analysis of an
`evolved transketolase reveals divergent binding modes. Scientific Reports, 6, 35716
`
`39. Barata T.S., Zhang C., Dalby P.A., Brocchini S., Zloh M. (2016) Int J. Mol. Sci. 17(6):853. Identification
`of Protein–Excipient Interaction Hotspots Using Computational Approaches. doi:10.3390/ijms17060853
`
`40. P. Morris, L. Rios-Solis, R. García-Arrazola, G. J. Lye, P. A. Dalby. (2016) Enzyme and Microbial
`Technology 89:85-91. Impact of cofactor-binding loop mutations on thermotolerance and activity of E. coli
`transketolase. doi:10.1016/j.enzmictec.2016.04.003
`
`41. F. Subrizi, M. Cardenas-Fernandez, G. J. Lye, J. M. Ward, P. A. Dalby, T. D. Sheppard, H. C. Hailes
`(2016) Green Chemistry. 18:3158-3165. Transketolase catalysed upgrading of L-arabinose: the one-step
`stereoselective synthesis of L-gluco-heptulose. DOI: 10.1039/C5GC02660A
`
`42. Chakroun N, Hilton D, Ahmad S, Platt G, Dalby PA. (2016) Molecular Pharmaceutics. 13(2):307-319.
`Mapping the Aggregation Kinetics of a Therapeutic Antibody Fragment. DOI:
`10.1021/acs.molpharmaceut.5b00387
`
`43. Dawid Deszcz, Pierre Affaticati, Nadine Ladkau, Alex Gegel, John M. Ward, Helen C. Hailes, Paul A.
`Dalby. (2015) FEBS J. 13:2512-2526. Single active-site mutants are sufficient to enhance serine:pyruvate
`α-transaminase activity in an ω-transaminase. DOI: 10.1111/febs.13293
`
`
`44. Payongsri P, Steadman D, Hailes HC, Dalby PA. (2015) Enzyme Microb Technol 71:45-52. Second
`generation engineering of transketolase for polar aromatic aldehyde substrates.
`10.1016/j.enzmictec.2015.01.008
`
`
`45. Lucy E. Rayner, Gar Kay Hui, Jayesh Gor, Richard K. Heenan, Paul A. Dalby, Stephen J. Perkins (2015) J
`Biol Chem 290: 8420-8438. The solution structures of two human IgG1 antibodies show conformational
`stability and accommodate their C1q and FCγR ligands.
`
`
`46. Rios-Solis L, Morris P, Grant C, Odeleye A, Hailes HC, Ward JM, Dalby PA, Baganz F, Lye GJ (2015)
`Chem Eng Sci 122:360-372. Modelling and optimisation of the one-pot, multi-enzymatic synthesis of chiral
`amino-alcohols based on microscale kinetic parameter determination
`
`
`47. Du CJ, Rios-Solis L, Ward JM, Dalby PA, Lye GJ (2014) Biocatalysis and Biotransformation 32, 302-313.
`Evaluation of CV2025 w-transaminase for the bioconversion of lignin breakdown products into value-
`added chemicals: synthesis of vanillylamine from vanillin
`
`
`48. Chris Grant, Dawid Deszcz, Yu-Chia Wei, Rubéns Julio Martínez-Torres, Phattaraporn Morris, Thomas
`Folliard, Rakesh Sreenivasan, John Ward, Paul Dalby, John M. Woodley & Frank Baganz (2014) Scientific
`Reports 4, 5844. Identification and use of an alkane transporter plug-in for applications in biocatalysis and
`whole-cell biosensing of alkanes. Doi:10.1038/srep05844
`
`
`49. Lucy E. Rayner, Gar Kay Hui, Jayesh Gor, Richard K. Heenan, Paul A. Dalby and Stephen J. Perkins
`(2014) Journal of Biological Chemistry. 289(30):20740-20756. The Fab Conformations in the Solution
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`Structure of Human IgG4 restricts Access to its Fc Region: Implications for Functional Activity. doi:
`10.1074/jbc.M114.572404
`
`
`50. Dalby PA (2014). The role of directed protein evolution in synthetic biology. In Synthetic Biology, Volume
`1. pp 79-105. RSC Books. 10.1039/9781849737845-00079
`
`
`51. Sagar, D.M., Aoudjane, S., Gaudet, M., Aeppli, G., Dalby, P.A. (2013) Scientific Reports 3:2130. Optically
`Induced Thermal Gradients for Protein Characterization in Nanolitre-scale Samples In Microfluidic
`Devices. DOI:10.1038/srep02130
`
`
`52. Lucy E. Rayner, Nilufar Kadkhodayi-Kholghi, Jayesh Gor, Paul A. Dalby, Stephen J. Perkins, (2013) J.
`Mol. Biol. 425:506-523. The solution structure of rabbit IgG accounts for its interactions with the Fc
`receptor and complement C1q and its conformational stability.
`
`
`53. Polykarpou, E.M., Dalby, P.A., Papageorgiou, L.G. (2012) Biochemical Engineering Journal 67:186-193.
`Novel efficient optimisation systems for purification process synthesis.
`
`
`54. Polykarpou, E.M., Dalby, P.A., Papageorgiou, L.G. (2012) Chemical Engineering Research and Design.
`90(9):1262-1270. An MILP formulation for the synthesis of protein purification processes.
`DOI: 10.1016/j.cherd.2011.11.021.
`
`
`55. Grant, Y., Dalby, P.A., Matejtschuk, P. (2012) American Pharmaceutical Review. 15(2):75-80. Use of
`design of experiment and micro-scale down strategies in formulation and cycle development for
`lyophilization.
`
`
`56. Panwajee Payongsri, David Steadman, John Strafford, Andrew MacMurray, Helen C. Hailes, Paul A.
`Dalby (2012) Org. Biomol. Chem. 10(45):9021-9029. Rational substrate and enzyme engineering of
`transketolase for aromatics. DOI: 10.1039/c2ob25751c.
`
`
`57. Ordidge G.C., Mannall, G., Liddell, J., Dalby, P.A., Micheletti, M. (2012) Biotechnology Progress
`28(2):435-444. A generic hierarchical screening method for the analysis of microscale refolds using an
`automated robotic platform. DOI: 10.1002/btpr.1502
`
`
`58. Robert Patterson, Jessica Nerren, Michael Kogut, Pinar Court, Bernardo Villarreal-Ramos, Hans-Martin
`Seyfert, Paul A. Dalby, Dirk Werling (2012) Developmental & Comparative Immunology 37(1):107-114.
`Yeast-surface expressed BVDV E2 protein induces a Th1/Th2 response in naïve T cells.
`DOI: 10.1016/j.dci.2011.10.009
`
`
`59. Grant, Y., Matejtschuk, P., Bird, C., Wadhwa, M., Dalby, P.A. (2012) Biotechnol. Letts. 34(4):641-648.
`Rapid identification and optimisation of freeze drying formulation using microscale and design of
`experiment approaches: A case study using granulocyte colony stimulating factor (G-CSF). DOI:
`10.1007/s10529-011-0822-2
`
`
`60. John Strafford, Panwajee Payongsri, Edward G. Hibbert, Phattaraporn Morris, Sukhjeet S. Batth, David
`Steadman, Mark E. B. Smith, John Ward, Helen C. Hailes, Paul A. Dalby (2012) J. Biotechnol. 157(1):
`237-245. Directed evolution to re-adapt a co-evolved network within an enzyme.
`DOI: 10.1016/j.jbiotec.2011.11.017
`
`
`61. Polykarpou, E.M., Dalby, P.A., Papageorgiou, L.G. (2012) Biotechnology Progress. 27(6): 1653-1660.
`Optimal synthesis of chromatographic trains for downstream protein processing. DOI: 10.1002/btpr.670
`
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`62. Dalby PA, Ward JM, and Hailes HC (2011). C-C Bond Formation Using TDP Dependent Enzymes. In
`Comprehensive Chirality, Ed, Hisashi Yamamoto and Erick Carreira. Elsevier, Oxford, UK.
`
`
`63. L. Rios-Solis, M. Halim, A. Cázares, P. Morris, J. M. Ward, H.C. Hailes, P.A. Dalby, F. Baganz, G. J. Lye
`(2011) Biocatalysis and Biotransformation.29(5):192-203. A toolbox approach for the rapid evaluation of
`multi-step enzymatic syntheses comprising a “mix and match” E. coli expression system with microscale
`experimentation. DOI: 10.3109/10242422.2011.609589
`
`
`64. Parker, B.M., Taylor, I., Woodley, J.M., Ward, J.M., Dalby, P.A. (2011) J. Biotechnol. 155(4):396-405.
`Directed evolution of a thermostable L-aminoacylase biocatalyst. DOI:10.1016/j.jbiotec.2011.07.029
`
`
`65. Jahromi, R.R.F., Morris, P., Martinez-Torres, R.J., Dalby, P.A. (2011) J. Biotechnol.155(2):209-216.
`Structural stability of E. coli transketolase to temperature and pH denaturation. DOI
`10.1016/j.jbiotec.2011.06.023
`
`
`66. Dalby, P. and DeGrado B. (2011) Current Opinion in Structural Biology 21:450-451. Engineering and
`Design. DOI: 10.1016/j.sbi.2011.06.003
`
`
`67. Dalby, P.A. (2011) Current Opinion in Structural Biology 21:473-480. Strategy and success for the
`directed evolution of enzymes. DOI 10.1016/j.sbi.2011.05.003
`
`
`68. Lye, G.J., Dalby, P.A., Baganz, F., Ward, J.M and Hailes, H.C. (2011). Biocatalysis is the future… the
`future is now! The Chemical Engineer, issue 839 (May), 36-38.
`
`
`69. Mahendrarajah, K., Dalby, P.A., Wilkinson, B. Jackson, S.E. & Main, E.R.G. (2011) Anal Biochem
`411:155-157. A high throughput fluorescence chemical denaturation assay as a general screen for protein-
`ligand binding. DOI 10.1016/j.ab.2010.12.001
`
`
`70. Ahmad, S.S., Dalby, P.A. (2011) Biotechnol. Bioeng. 108(2):322-332. Thermodynamic parameters for salt-
`induced reversible protein precipitation from automated microscale experiments. (DOI: 10.1002/bit.22957)
`
`
`71. Hailes, H.C, Dalby, P.A., Lye, G. J., Baganz, F., Micheletti, M., Szita, N., Ward, J.M. (2010) Current
`Organic Chemistry. 14(17):1883-1893. α,α'-Dihydroxy Ketones and 2-Amino-1,3-diols: Synthetic and
`Process Strategies using Biocatalysts.
`
`
`72. James L. Galman, David Steadman, Sarah Bacon, Phattaraporn Morris, Lydia G. Crago, Mark E.B. Smith,
`John Ward, Paul A. Dalby and Helen C. Hailes (2010) ChemComm 46(40):7608-7610. α,α’-
`Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase
`enzymes.
`
`
`73. Abe, Y., Gor, J., Bracewell, D.G., Perkins, S.J., Dalby, P.A. (2010) Biochem. J. 432(1):101-111. Masking
`of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody
`function and therapy. (DOI: 10.1042/BJ20100641)
`
`
`74. Gaudet, M., Remtulla, N., Jackson, S.E., Main, E.R.G., Bracewell, D.G., Aeppli, G., Dalby, P.A. (2010)
`Protein Science. 19, 1544-1554. Protein denaturation and protein:drug interactions from intrinsic protein
`fluorescence measurements at the nanolitre scale. (DOI: 10.1002/pro.433)
`
`
`75. Cázares, A., Galman, J.L., Crago, L.G., Smith, M.E.B., Strafford, J., Ríos-Solís, L., Lye, G.J., Dalby, P.A.,
`Hailes, H.C. (2010) Organic & Biomolecular Chemistry. 8(6), 1301-1309. Non-alpha-hydroxylated
`aldehydes with evolved transketolase enzymes DOI: 10.1039/b924144b Cover of issue 8(6) 2010, March
`21.
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`MPI EXHIBIT 1025 PAGE 8
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`76. Smith, M.E.B., Chen, B.H., Hibbert, E.G., Kaulmann, U., Smithies, K., Galman, J.L., Baganz, F., Dalby,
`P.A., Hailes, H.C., Lye, G.J., Ward, J.M., Woodley, J.M., Micheletti, M. (2010) Org. Proc. Res. Dev.
`14(1), 99-107. A Multi-Disciplinary Approach Toward the Rapid and Preparative Scale Biocatalytic
`Synthesis of Chiral Amino Alcohols: A Concise Transketolase / ω-Transaminase-Mediated Synthesis of
`(2S,3S)-2-Aminopentane-1,3-diol. (10.1021/op900190y)
`
`
`77. Wohlgemuth R, Smith MEB, Dalby PA and Woodley JM (2010) Transketolases. In Encyclopedia of
`Industrial Biotechnology: Bioprocess, Bioseparation and Cell Technology, Ed, Michael C. Flickinger, Vol.
`7 pp 4746-4752. John Wiley and Sons, Inc, Hoboken.
`
`
`78. Grant, Y., Matejtschuk, P., Dalby, P.A. (2009) Biotech. Bioeng. 104(5), 957-964. Rapid optimisation of
`protein freeze-drying formulations using ultra scale-down and factorial design of experiment in
`microplates. (DOI 10.1002/bit.22448)
`
`
`79. Hailes, H.C., Dalby. P.A., Lye, G.J., Ward, J.M. (2009) Chim Oggi. 27(4), 28-31. Biocatalytic Approaches
`to Ketodiols and Aminodiols.
`
`
`80. Dalby. P.A., Baganz, F.B., Lye, G.J., Ward, J.M. (2009) Chim Oggi. 27(4), 18-22. Protein and pathway
`engineering in biocatalysis.
`
`
`81. Dalby, P. (2009) J. Biomolecular Screening 14(7), 900-900. High-Throughput Characterization of Proteins
`From Microtiter Plates to Microfluidics. 15th Annual Conference and Exhibition of the Society-of-
`Biomolecular-Sciences, APR 26-30, 2009 Lille, France.
`
`
`82. Paramesvaran, J., Hibbert, E.G., Russell, A.J., Dalby, P.A. (2009) Protein Engineering Design and
`Selection. 22(7), 401-411. Distributions of enzyme residues yielding mutants with improved substrate
`specificities from two different directed evolution strategies. (DOI 10.1093/protein/gzp020)
`
`
`83. Mannall, G.J, Myers, J.P., Liddell J., Titchener-Hooker, N.J., Dalby, P.A. (2009) Biotech. Bioeng. 103(2),
`329-340. Ultra scale-down of protein refold screening in microwells: Challenges, solutions and application.
`(DOI 10.1002/bit.22245)
`
`
`84. Polykarpou, E.M., Dalby, P.A. and Papageorgiou, L.G. (2009) An MINLP formulation for the synthesis of
`chromatographic protein purification processes with product loss. 19th European Symposium on Computer
`Aided Process Engineering – ESCAPE19. J. Jeżowski and J. Thullie (Editors)
`
`
`85. Smith, M.E.B., Hibbert, E.G., Jones, A.B., Dalby, P.A, and Hailes, H.C. (2008) Adv Syn Catal 350, 2631-
`2638. Enhancing and reversing the enantioselectivity of E. coli transketolase via single point
`mutations. (DOI 10.1002/adsc.200800489)
`
`
`86. Chen, B.H., Hibbert, E.G., Dalby, P.A., and Woodley, J.M. (2008) AIChE J. 58, 2155-2163. A new
`approach to bioconversion reaction kinetic parameter identification. (DOI 10.1002/aic.11545)
`
`
`87. Hibbert, E.G., Senussi, T., Smith, M.E.B., Costelloe, S.J., Ward, J.M., Hailes, H.C., and Dalby, P.A.
`(2008) J. Biotechnol. 134, 240-245. Directed evolution of transketolase substrate specificity towards an
`aliphatic aldehyde. (DOI 10.1016/j.jbiotec.2008.01.018)
`
`
`88. Aucamp, J.P., Martinez-Torres, R.J., Hibbert, E.G. and Dalby, P.A. (2008) Biotech. Bioeng. 99, 1303-1310.
`A microplate-based evaluation of complex denaturation pathways: Structural stability of E. coli
`transketolase. (DOI 10.1002/bit.21705) Cover of issue 99(6) 2008, April 15.
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`MPI EXHIBIT 1025 PAGE 9
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`89. Costelloe, S.J., Ward, J.M., and Dalby, P.A. (2008) J. Mol. Evol. 66, 36-49. Evolutionary analysis of the
`TPP-dependent enzyme family. DOI 10.1007/s00239-007-9056-2)
`
`
`90. Dalby, P.A., Aucamp, J.P., George, R. and Martinez-Torres, R.J. (2007) Biochem. Soc. Transact. 35, 1606-
`1609. Structural stability of an enzyme biocatalyst. DOI:10.1042/BST0351606
`
`
`91. Hibbert, E.G., Senussi, T., Costelloe, S.J., Lei, W., Smith, M.E.B., Ward, J.M., Hailes, H.C., and Dalby,
`P.A. (2007) Journal of Biotechnology 131, 425-432. Directed evolution of transketolase activity on non-
`phosphorylated substrates. (10.1016/j.jbiotec.2007.07.949)
`
`
`92. Hailes, H.C., Dalby, P.A. and Woodley, J.M. (2007) J. Chem. Technol. Biotechnol. 82, 1063–1066.
`Integration of biocatalytic conversions into chemical syntheses.
`
`
`93. Miller, O.J., Hibbert, E.G., Ingram, C.U., Lye, G.J., Dalby, P.A. (2007) Biotechnol. Letts. 29(11):1759-
`1770. Optimisation and evaluation of a generic microplate-based HPLC screen for
`transketolase. DOI: 10.1007/s10529-007-9435-1
`
`
`94. Martinez-Torres, R.J., Aucamp, J.P., George, R. and Dalby, P.A. (2007) Enzyme Microb. technol. 41, 653-
`662. Structural stability of E. coli transketolase to urea denaturation. (DOI:
`10.1016/j.enzmmictec.2007.05.019)
`
`
`95. Mannall, G. J., Titchener-Hooker, N. J. and Dalby, P. A. (2007) Biotech. Bioeng. 97, 1523-1534. Factors
`affecting protein refolding yields in a fed-batch and batch-refolding system. (DOI: 10.1002/bit.21377)
`
`
`96. Dalby, P. A. (2007) Recent Patents Biotechnol. 1 (1), 1-9. Engineering enzymes for biocatalysis.
`
`97. Ingram, C. U., Bommer, M., Smith, M. E. B., Dalby, P. A., Ward, J. M., Hailes, H.C., Lye, G. J. (2007)
`Biotech. Bioeng. 96, 559-569. One-pot synthesis of amino-alcohols using a de-novo transketolase and -
`alanine:pyruvate transaminase pathway in Escherichia coli. DOI: 10.1002/bit.21125
`
`
`98. Dimitriadou, E.K., Lye, G.J., Dalby, P.A. (2007) Redesign and altered ion-binding of a natural calcium
`regulated biosensor, photoprotein aequorin. Amino Acids 33 (3): XLVI-XLVI.
`
`
`99. Baganz, F., Chen, B., Dalby, P., Hibbert, E., Lye, G., Micheletti, M., Woodley, J., Kaulmann U., Ward, J.,
`Hailes, H., Smith, M. and Smithies, K. (2007) Accelerating biocatalytic process design: Integrating new
`tools from biology, chemistry and engineering. J. Biotechnol. 131 S78-S78.
`
`
`100. Chen, B. H., Sayar, A., Kaulmann, U., Dalby, P. A., Ward, J. M. and Woodley, J. M. (2006) Biocatal.
`Biotrans. 24, 449-457. Reaction m