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`HEALTH SCIENCES LIBRARY
`UNIVERSITYOFWISCONSIN
`
`SIKTY-SIKTA
`annual meeting
`of the
`American Association
`for Cancer Research
`
`PROCEEDINGS
`
`ELEVENTH
`annual meeting
`of the
`American Society
`ot Clinical Oncology
`MAY ZIl, IGA
`San Diego, Calfornio
`
`
`
`Volume 16: March 1975
`
`CELGENE 2126
`CELGENE 2126
`APOTEX v. CELGENE
`“APOTEX v. CELGENE
`IPR2023-00512
`IPR2023-00512
`
`

`

`Proceedings of the
`American Association for Cancer Research
`and
`American Society of Clinical Oncology
`
`Sidney Weinhouse, Editor
`
`Margaret Foti, Managing Editor
`
`
`AMERICAN ASSOCIATION FOR CANCER RESEARCH, INC.
`
`OFFICERS
`
`(1974-1975)
`
`President, Van R. Potter
`
`Vice President, Charlotte Friend
`
`Secretary-Treasurer, Hugh J. Creech
`
`BOARD OF DIRECTORS
`
`Term Expiring May 1975
`
`ThomasC, Hall
`G. A. LePage
`
`Carl G. Baker
`Paul Calabresi
`
`Term Expiring May 1976
`
`Van R., Potter
`Frank M. Schabel, Jr.
`
`Charlotte Friend
`Ludwik Gross
`
`Term Expiring May 1977
`
`Seymour S. Cohen
`George H. Hitchings
`
`Mortimer L. Mendelsohn
`Elizabeth C. Miller
`
`Ex Officio
`
`Sidney Weinhouse
`Hugh J. Creech
`
`
`AMERICAN SOCIETY OF CLINICAL ONCOLOGY, INC.
`
`OFFICERS
`
`(1974-1975)
`
`President, Rose Ruth Ellison
`
`President Elect, Joseph R. Bertino
`
`Immediate Past President, Bayard D. Clarkson
`
`Secretary-Treasurer, Audrey E. Evans
`
`BOARD OF DIRECTORS
`
`Daniel Bergsagel
`
`Vincent T. DeVita
`
`Albert H. Owens, Jr.
`
`
`Copyright 1975 by Cancer Research, Inc. Published for Cancer Research, Inc., and the
`American Association for Cancer Research, Inc., by The Williams & Wilkins Co., Baltimore,
`Md. 21202, and included in subscriptions to the journal CANCER RESEARCH.
`
`

`

`617 REMISSION INDUCTION IN REFRACTORY MYELO-
`BLASTIC LEUKEMIA WITH CONTINUOUS INFU-
`SION OF 5-AZACYTIDINE. W.R. Vogler, D. Miller
`and J.W. Keller. Emory Univ., Atlanta, Ga.
`30322 and Duke Univ., Durham, N.C. 27710
`(For Southeastern Cancer Study Group)
`Rapid infusions of 5-azacytidine in
`doses of 100mg/M2 or greater produce severe
`nausea, vomiting and occasional diarrhea,
`thus limiting its therapeutic use.
`In this
`study doses of 50-400mg/M2/day were infused
`continuously for 5-day courses at intervals
`of 14-25 days. The use of Ringer's lactate
`and frequent (q3-I2hr) changes of solutions
`insured drug stability. The majority of
`patients, all with refractory myeloblastic
`Jeukemia, received 150mg/M2/day. Doses were
`escalated by 50mg/M2 increments in 6 of 22
`patients. All 22 patients received at least
`2 courses of treatment. Complete remissions
`occurred in 7(32%) and partial remissions in
`3(14%).
`In general
`the drug was well
`toler-
`ated, however, nauSea and vomiting became
`troublesome at doses of 200mg/M2 or more.
`The results indicate that 5-azacytidine
`given by continuous infusion is an active
`agent in the treatment of refractory myelo-
`blastic leukemia and worthy of further in-
`vestigation.
`
`61
`
`CYLINDROID LAMELLA-PARTICLE COMPLEXES IN
`MALIGNANT LYMPHOMA OF NORTHERN PIKE (E.
`lucius). Clyde J. Dawe, William G. Banfield,
`Cecil W. Lee, Herman J. Michelitch, and Ron
`Sonstegard. National Institutes of Health,
`Bethesda, Md. 20014 and Univ. of Guelph,
`Ontario, Canada N1G 2W1
`Malignant lymphoma in northern pike is
`enzootic in North America, Ireland, and Sweden.
`Experimental and field studies have indicated
`the disease is transmissible. We report intra-
`cytoplasmic cylindroid structures visualized by
`transmission electron microscopy in pike lymph-
`oma cells. Cylindroid bodies range from 2 to
`4 um.
`in length. and 0.8 to 1.0 pm.
`in diameter.
`Their walls are composed of alternating layers
`of dense lamellae and dense particles.
`The
`dense lamellae average about 90 A° in thickness
`and the particles about 260 A? in diameter.
`Often the inner and outer surfaces of the walls
`of the cylindroids are covered by two wavy
`membranes enclosing a space similar to the
`perinuclear cisterna.
`The cylindroids in pike
`lymphoma cells resemble those described in
`human "hairy cell" leukemia and in EB virus-
`transformed human embryo cells. Their sig-
`nificance and manner of development are not
`understood.
`One concept is that the particles
`are RNA and the membranes are part of the
`endoplasmic reticulum: Another possibility is
`that the particles are DNA and the membranes
`are derived from nuclear membrane.
`
`AACR Abstracts, 1975
`
`IMPROVEMENT
`619 MULTIPLE LEUKOCYTE WASHING:
`IN CELL~MEDIATED IMMUNITY IN LUNG CAN-
`CER. Patrick C. Marabella, Hiroshi Takita,
`Mitsuru Takada and Jun Minowada. Roswell
`Park Memorial
`Institute, Buffalo, N.Y. 14203
`This study was undertaken to investigate
`the possibility of a "blocking factor"! adhe-
`rent
`to leukocytes in lung cancer.
`The leukocyte migration inhibition test
`using leukocytes isolated from heparinized
`peripheral blood of 28 previously untreated
`lung cancer patients was employed.
`The leu-
`kocytes were washed comparatively three and
`six times.
`The antigen used was a tumor
`extract of a cultured cell
`line of lung car-
`cinoma. Twenty-five of the 28 cases had im-
`proved percent
`inhibition with six times
`wash.
`The overall percent migration inhi-
`bition of the three times washed group was
`-1.3 + 4.4% and that of the six times washed
`group being 13.9 + 4.6%.
`The 11
`localized
`cases had percentinhibitions of 14.3 + 4.5
`and 26.4 + 7.1% for three and six times
`washed groups respectively.
`The 17 dissem-
`inated cases had percent
`inhibition of -12.5
`+ 6.8% and 4.5 + 3.9% for three and six times
`washed.
`~
`It appears that multiple washing of
`leukocytes improves in vitro cellular immunity
`in lung cancer patients and moreso in dissem-
`inated localized cases.
`Impaired cellular
`immunity in lung cancer may be due to some
`"blocking factor’! adherent
`to the leukocytes.
`
`620 PATTERNS OF SPLEEN CELL (SC) MIGRATION
`IN VITRO IN SPONTANEOUSLY METASTASIZING
`AND NON-METASTASTZING MAMMARY TUMOR (MT) HOSTS:
`POSSIBLE IMMUNE ESCAPE MECHANISM. U. Kim and
`G. Montessori. Roswell Park Memorial Institute,
`Buffalo, N. Y. 14203
`In vitro demonstration of positive lym
`phocyte cytotoxicity in patients with a rapidly
`growing or widely disseminated cancer is one of
`the most puzzling phenomena in tumor immunology.
`To gain a better understanding of this immune
`response in a well defined experimental systen,
`the patterns of SC migration and migration in-
`hibition were studied by the standard MIF test
`procedure with 4 spontaneonsly metastasizing,
`antigen-shedding and in 3 non-metastasizing,
`non-shedding rat MT.
`The SC from non-metasta-
`sizing MT hosts migrated as well as normal SC
`or was often slightly stinmmlated in the blank
`culture media. However, SC migration from: the
`metastasizing MT hosts was markedly inhibited
`(40-60%) in the media indicating that their
`physical capacity to migrate was impaired. By
`incubating the SC from the former group with
`their respective MT extract, their migration
`was inhibited by 30-40%. On the other hand,
`the inhibited SC migration in the latter group
`was usually restored to normal levels or even
`stimulated upon exposure to their own MT ex-
`tract. This inhibition or stimulation was
`tumor specific.
`The significance of "blocking" and "un-
`blocking" effects of specific MT antigens will
`be discussed in terms of both antigen-antibody
`on the lymphocyte surface and the relative mi-
`gration patterns between SC and thymus cells.
`
`MARCH 1975
`
`155
`
`