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`A purified inactivated Japanese encephalitis virus vaccine made in Vero cells - PubMed
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`A purified inactivated Japanese encephalitis virus
`vaccine made in Vero cells
`
`Abstract
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`. 2001 Aug 14;19(31):4557-65. doi: 10.1016/s0264-410x(01)00208-0.
`A K Srivastava , J R Putnak, S H Lee, S P Hong, S B Moon, D A Barvir, B Zhao, R A Olson, S O Kim,
`W D Yoo, A C Towle, D W Vaughn, B L Innis, K H Eckels
`Affiliations
`PMID: 11483284 DOI: 10.1016/s0264-410x(01)00208-0
`
`Vaccine
`1
`A second generation, purified, inactivated vaccine (PIV) against Japanese encephalitis (JE) virus
`was produced and tested in mice where it was found to be highly immunogenic and protective. The
`JE-PIV was made from an attenuated strain of JE virus propagated in certified Vero cells, purified,
`and inactivated with formalin. Its manufacture followed current GMP guidelines for the production
`of biologicals. The manufacturing process was efficient in generating a high yield of virus,
`essentially free of contaminating host cell proteins and nucleic acids. The PIV was formulated with
`aluminum hydroxide and administered to mice by subcutaneous inoculation. Vaccinated animals
`developed high-titered JE virus neutralizing antibodies in a dose dependent fashion after two
`injections. The vaccine protected mice against morbidity and mortality after challenge with live,
`virulent, JE virus. Compared with the existing licensed mouse brain-derived vaccine, JE-Vax, the
`Vero cell-derived JE-PIV was more immunogenic and as effective as preventing encephalitis in
`mice. The JE-PIV is currently being tested for safety and immunogenicity in volunteers.
`MedGen
`PubChem Compound (MeSH Keyword)
`Elsevier Science
`The Lens - Patent Citations
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`11/10/22, 8:02 PM
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`11483284
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`PMID- 11483284
`OWN - NLM
`STAT- MEDLINE
`DCOM- 20010927
`LR - 20191210
`IS - 0264-410X (Print)
`IS - 0264-410X (Linking)
`VI - 19
`IP - 31
`DP - 2001 Aug 14
`TI - A purified inactivated Japanese encephalitis virus vaccine made in Vero
`PG - 4557-65
`AB - A second generation, purified, inactivated vaccine (PIV) against Japanes
` encephalitis (JE) virus was produced and tested in mice where it was fou
` highly immunogenic and protective. The JE-PIV was made from an attenuate
` of JE virus propagated in certified Vero cells, purified, and inactivate
` formalin. Its manufacture followed current GMP guidelines for the produc
` biologicals. The manufacturing process was efficient in generating a hig
` of virus, essentially free of contaminating host cell proteins and nucle
` The PIV was formulated with aluminum hydroxide and administered to mice
` subcutaneous inoculation. Vaccinated animals developed high-titered JE v
` neutralizing antibodies in a dose dependent fashion after two injections
` vaccine protected mice against morbidity and mortality after challenge w
` virulent, JE virus. Compared with the existing licensed mouse brain-deri
` vaccine, JE-Vax, the Vero cell-derived JE-PIV was more immunogenic and a
` effective as preventing encephalitis in mice. The JE-PIV is currently be
` tested for safety and immunogenicity in volunteers.
`FAU - Srivastava, A K
`AU - Srivastava AK
`AD - Walter Reed Army Institute of Research, Division of Communicable Disease
` Immunology, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
`FAU - Putnak, J R
`AU - Putnak JR
`FAU - Lee, S H
`AU - Lee SH
`FAU - Hong, S P
`AU - Hong SP
`FAU - Moon, S B
`AU - Moon SB
`FAU - Barvir, D A
`AU - Barvir DA
`FAU - Zhao, B
`AU - Zhao B
`FAU - Olson, R A
`AU - Olson RA
`FAU - Kim, S O
`AU - Kim SO
`FAU - Yoo, W D
`AU - Yoo WD
`FAU - Towle, A C
`AU - Towle AC
`FAU - Vaughn, D W
`AU - Vaughn DW
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`FAU - Innis, B L
`AU - Innis BL
`FAU - Eckels, K H
`AU - Eckels KH
`LA - eng
`PT - Journal Article
`PL - Netherlands
`TA - Vaccine
`JT - Vaccine
`JID - 8406899
`RN - 0 (Japanese Encephalitis Vaccines)
`RN - 0 (Vaccines, Inactivated)
`RN - H2D2X058MU (Cyclic GMP)
`SB - IM
`MH - Animals
`MH - Chlorocebus aethiops
`MH - Cyclic GMP/biosynthesis
`MH - Drug Evaluation, Preclinical
`MH - Encephalitis Virus, Japanese/genetics/*immunology/isolation & purificati
`MH - Encephalitis, Japanese/*prevention & control
`MH - Female
`MH - Japanese Encephalitis Vaccines/administration & dosage/*biosynthesis/iso
` purification
`MH - Mice
`MH - Mice, Inbred ICR
`MH - Serial Passage
`MH - Vaccines, Inactivated/administration & dosage/biosynthesis/isolation &
` purification
`MH - Vero Cells
`MH - Virus Replication
`EDAT- 2001/08/03 10:00
`MHDA- 2001/09/28 10:01
`CRDT- 2001/08/03 10:00
`PHST- 2001/08/03 10:00 [pubmed]
`PHST- 2001/09/28 10:01 [medline]
`PHST- 2001/08/03 10:00 [entrez]
`AID - S0264-410X(01)00208-0 [pii]
`AID - 10.1016/s0264-410x(01)00208-0 [doi]
`PST - ppublish
`SO - Vaccine. 2001 Aug 14;19(31):4557-65. doi: 10.1016/s0264-410x(01)00208-0
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