Curriculum Vitae
`
`DAVID HOWARD SHERMAN
`Address
`
`University of Michigan
`Life Sciences Institute
`210 Washtenaw Avenue
`Ann Arbor, Michigan 48109-2216
`734-615-9907 (FAX: 734-615-3641)
`Email: davidhs@umich.edu
`
`Education
`
`B.A. in Chemistry (with Honors) from the University of California, Santa Cruz, CA: 1975 - 1978
`Research Advisor: Professor Phil Crews
`
`Ph.D. in Organic Chemistry from Columbia University, New York City, NY; 1978 - 1981
`Research Advisor: Professor Gilbert Stork
`
`Professional Appointments
`
`1981 - 1982
`1982 - 1984
`1984 - 1987
`1987 - 1990
`1990 - 1995
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`1995 – 2000
`
`1996 – 1998
`
`Postdoctoral, Yale University, New Haven, CT (R.E. Handschumacher)
`Postdoctoral, Massachusetts Institute of Technology, Cambridge, MA (H.N. Eisen)
`Research Scientist, Biogen Research Corp., Cambridge, MA
`Research Scientist, John Innes Institute, Norwich, U.K.
`Assistant Professor, University of Minnesota, Department of Microbiology and
`BioTechnology Institute
`Associate Professor, University of Minnesota, Department of Microbiology and
`BioTechnology Institute
`Director, University of Minnesota, Center for Microbial Physiology and Metabolic
`Engineering
`Senior Director, ChromaXome Corporation, San Diego, CA
`(sabbatical)
`Director, Microbiology, Immunology and Cancer Biology Graduate Program
`Founder and Chief Technical Consultant, Acera Biosciences, Inc.
`Director, UMN-NIGMS Biotechnology Training Program
`Professor, University of Minnesota, Department of Microbiology and BioTechnology
`Institute
`J. G. Searle Professor, University of Michigan, Department of Medicinal Chemistry
`Director, Center for Chemical Genomics, Life Sciences Institute, University of
`Michigan
`Professor, University of Michigan, Department of Chemistry
`2003 – present
`Professor, University of Michigan, Department of Microbiology & Immunology
`2003 – present
`2007 – present Hans W. Vahlteich Professor, University of Michigan, Department of Medicinal
`Chemistry
`Associate Dean for Research and Graduate Education (College of Pharmacy)
`2011 – 2016
`2016 – present Microbiology Spectrum Advisory Board, American Society for Microbiology Press
`1
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`1997
`1998 – 2001
`1999 – 2007
`2000 – 2003
`2000 – 2003
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`2003 – 2007
`2004 – 2014
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`2017 – present Member Editorial Board, Journal of Biological Chemistry, American Society for
`Biochemistry and Molecular Biology
`2021 – present Associate Editor, Engineering Microbiology (Elsevier Press)
`
`Honors and Awards
`
`
`1978
`
`1978
`
`1981
`1982 - 1984
`1984 - 1986
`1990 - 1992
`1992 - 1995
`2003 - 2007
`2005-
`
`American Chemical Society Award for Excellence in Undergraduate Research
`University of California, Santa Cruz, Honors in the Major (Chemistry)
`Pegram Award for Excellence in Graduate Research, Columbia University
`National Institutes of Health Postdoctoral Fellowship
`Myron A. Bantrell Postdoctoral Research Fellowship in Molecular Biology
`Eli Lilly Life Sciences Award
`Procter & Gamble University Exploratory Research Program
`John Gideon Searle Jr. Professorship, University of Michigan
`Co-chair, University of Miami Oceans and Human Health Center External Advisory
`Committee
`Hans W. Vahlteich Professorship, University of Michigan
`Elected Fellow, American Association for the Advancement of Science
`A. C. Cope Scholar Award, American Chemical Society
`Charles Thom Award, Society for Industrial Microbiology
`American Society for Microbiology Distinguished Lecturer (2015-2017)
`
`2007-
`2008
`
`2009
`
`2009
`
`2015
`
`
`Select Named Lectureships and Major Symposia:
`
`2006
`2007
`
`Gordon Cragg Symposium Lecture, Missouri Botanical Garden
`Abbott Laboratories Distinguished Lecture, Notre Dame Univ., Dept. of Chemistry &
`Biochemistry
`Joseph F. Bunnett Lecture, UC Santa Cruz Department of Chemistry and Biochemistry
`National Academy of Sciences Peru (Lima, Peru)
` J. Clarence Karcher Lecture, Department of Chemistry and Biochemistry, University of
`Oklahoma
`Plenary Lecture, Korean Society for Microbiology (Seoul, Korea)
` Plenary Lecture, International Symposium for Natural Product Research, (New York,
`NY)
`Plenary Lecture, Directing Biosynthesis III, (Nottingham, UK)
`Foster Colloquium Lecture, University of Buffalo, Department of Chemistry
`Plenary Lecture, University of Colorado Symposium on Translating Structural Biology to
`Medicine
`Plenary Lecture, Drexel Univ. School of Medicine, Symposium on Metagenomics &
`Synthetic Biology
`C. Richard Hutchinson Lecture, University of Wisconsin-Madison
`Distinguished Organic Research Lecture, University of British Columbia
`Madeleine Joullié Lecture, University of Pennsylvania, Department of Chemistry
`Melvin Calvin Lecture, University of California, Berkeley, Department of Chemistry
`Plenary Lecture, Genetics of Industrial Microorganisms Symposium (Wuhan, China)
`Plenary Lecture, International Symposium for the Biology of Actinomycetes (Jeju,
`Korea)
`Plenary Lecture, Bristol-Myers Squibb Company Green Chemistry Symposium
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`
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`2010
`2010
`2012
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`2012
`2012
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`2012
`2012
`2012
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`2013
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`2014
`2015
`2015
`2016
`2016
`2017
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`2017
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`2017
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`2019
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`2020
`2020
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`2020
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`
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`4th Annual General Electric Santé Lecture in Biochemistry, McGill University
`(Montreal, Canada)
`Keynote Speaker, Copenhagen Bioscience Conference; Natural Products Discovery,
`Biosynthesis and Application (Novo Nordisk Foundation, Copenhagen, Denmark)
`Leonard Katz Symposium, University of California, Berkeley (Berkeley, CA)
`Plenary Lecture, Korean Society for Biotechnology and Bioengineering (virtual via
`Zoom)
`Indiana University/Purdue University Indianapolis, Corteva, Eli Lilly Symposium (virtual
`via Zoom)
`
`
`Research Experience and Interests
`Undergraduate Research (U.C. Santa Cruz): A novel GC/MS method was developed for the identification
`and characterization of polysaccharide natural products from marine alga.
`
`Graduate Research (Columbia University): A general method was developed for construction of trans-
`hydrindanone ring systems, and the total synthesis of 11-keto steroids using an intramolecular Diels-Alder
`approach.
`
`Postdoctoral Research (Yale University): Studies of the immunosuppressive drug cyclosporin A were
`performed to determine its effects on thymocyte populations in mice.
`
`Postdoctoral Research (MIT): Studies were conducted on non-H-2 class I molecules of the mouse major
`histocompatibility complex. Our work showed that specific subpopulations of T cells expressed
`structurally diverse molecules encoded in the Qa-2 locus.
`
`Biogen Research Corporation (Cambridge, MA): A molecular genetic study was conducted on insulin-
`specific, class II MHC restricted T cell receptors in mice. Further biochemical and molecular genetic
`studies were conducted on Qa-2 encoded MHC molecules.
`
`John Innes Institute (Norwich, U.K.): A molecular genetic analysis of type II polyketide synthase systems
`was conducted to explore the identity and mechanism of construction of complex secondary metabolites
`in Streptomyces.
`
`University of Michigan (Ann Arbor, MI): Research interests include synthetic chemistry, bioorganic and
`biochemical studies of natural product biosynthetic pathways from marine and terrestrial microbes.
`Metabolic engineering and microbial genomic and proteomic technologies are being developed and
`utilized for analysis and production of novel biologically active molecules and identification of drug
`targets.
`
`Professional Memberships
`
`American Chemical Society
`1978 - Present
`American Association for the Advancement of Science
`1982 - Present
`American Society for Microbiology
`1987 - Present
`Society for Industrial Microbiology
`2000 – Present
`2015 – Present American Association of Pharmaceutical Scientists
`2015 – Present American Society for Pharmacognosy
`2016 – Present American Heart Association
`
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`Publications
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`1.
`
`Stork, Gilbert and David H. Sherman. 1982. "Efficient de novo construction of the indanpropionic
`acid precursor of 11-keto steroids. An improved internal Diels-Alder sequence," J. Amer. Chem.
`Soc. 104:3758-3759.
`
`
`2. Kranz, David M., David H. Sherman, Michael V. Sitkovsky, Mark S. Pasternack, and Herman N.
`Eisen. 1984. "Immunoprecipitation of cell surface structures of cloned cytotoxic T lymphocytes by
`clone-specific antisera," Proc. Natl. Acad. Sci. USA 81:573-577. PMCID: PMC344721
`
`
`3.
`
`
`4.
`
`Sherman, David H. 1984. "Increasing sensitivity of luminescent enzyme immunoassay," Trends in
`Biotechnology 2:1-2.
`
`Sherman, David H., David M. Kranz, and Herman N. Eisen. 1984. "Expression of structurally
`diverse Qa-2 encoded molecules on the surface of cloned cytotoxic T lymphocytes," Journal of
`Experimental Medicine 160:1421-1430. PMCID: PMC2187513
`
`
`5. Devlin, James J., Georg Widera, Andrew L. Mellor, Karen Fahrner, David H. Sherman, Elisabeth
`H. Weiss, and Richard A. Flavell. 1985. "Evolution and expression of the transplantation antigen
`gene family," Federation Proceedings 44:2736-2740. PMID:3926542
`
`
`6.
`
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`7.
`
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`8.
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`Sherman, David H., David M. Kranz, and Herman N. Eisen. 1985. "Qa-2 encoded molecules
`expressed on the surface of cloned cytotoxic T lymphocytes are structurally diverse," The Cell
`Biology of the MHC, H. Vogel and B. Pernis (eds.), Academic Press, Inc., New York.
`
`Flavell, Richard A., Hamish Allen, Linda C. Burkly, David H. Sherman, Gerald L. Waneck, and
`Georg Widera. 1986. “Molecular biology of the H-2 complex," Science 233:437-443.
`
`Sherman, David H., Paula S. Hochman, Robert Dick, Richard Tizard, K.L. Ramachandran, Richard
`A. Flavell, and Brigitte T. Huber. 1987. "A molecular analysis of antigen recognition by insulin
`specific T cell hybridomas from B6 wild type and bm12 mutant mice," Molecular and Cellular
`Biology 7:1865-1872. PMCID: PMC365290
`
`
`9. Waneck, Gerald L., David H. Sherman, Susan Calvin, Hamish Allen, and Richard A. Flavell. 1987.
`"Tissue-specific expression of a transfected Qa region gene (Q7b) encoding the Qa-2 alloantigen,"
`Journal of Experimental Medicine 165:1358-1370. PMCID: PMC2188306
`
`
`10. Sherman, David H., Gerald L. Waneck, and Richard A. Flavell. 1988. "Qa-2 antigen encoded by
`Q7b transfected R1.1 cells is biochemically indistinguishable from Qa-2 expressed on the surface of
`C57B1/10 mouse spleen cells," Journal of Immunology 140:138-142. PMID:3335777
`
`
`11. Waneck, Gerald L., David H. Sherman, Paul W. Kincade, Martin G. Low, and Richard A. Flavell.
`1988. "Molecular mapping of sites in Qa-2 required for attachment of the phosphatidylinositol
`membrane anchor," Proc. Natl. Acad. Sci. USA 85:577-581. PMCID: PMC279594
`
`
`12. Sherman, D.H., F. Malpartida, M.J. Bibb, H.M. Kieser, S.E. Hallam, J.A. Robinson, S. Bergh, M.
`Uhlen, T.J. 1988. "Cloning and analysis of genes for the biosynthesis of polyketide antibiotics in
`Streptomyces species," in Durand, G., Bobichon, L. and Florent, J. (eds.) Proceedings of the 8th
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`International Biotechnology Symposium, Paris. Societe Francaise de Microbiology, Vol. 1, pp. 123-
`137.
`
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`13. Sherman, David H., Francisco Malpartida, Maureen J. Bibb, Helen M. Kieser, Mervyn J. Bibb, and
`David A. Hopwood. 1989. "Structure and deduced function of the granaticin-producing polyketide
`synthase gene cluster from Streptomyces violaceoruber Tu22," EMBO Journal 8:2717-2725.
`PMCID: PMC401279
`
`
`14. Hopwood, David A. and David H. Sherman. 1990. "Molecular genetics of polyketides and its
`comparison to fatty acid biosynthesis," Annual Review of Genetics 24:37-66. PMID:2088174
`
`
`15. Hopwood, David A., David H. Sherman, Chaitan Khosla, Maureen J. Bibb, Thomas J. Simpson,
`Miguel A. Fernandez, Eduardo Martinez and Francisco Malpartida. 1990. "Hybrid pathways for the
`production of secondary metabolites," in Proceedings of the Sixth International Symposium on the
`Genetics of Industrial Microorganisms (GIM 90), Strassbourg, France .
`
`
`16. Sherman, David H., M.J. Bibb, T.J. Simpson, D. Johnson, F. Malpartida, M. Fernandez-Moreno, E.
`Martinez, C.R. Hutchinson and D.A. Hopwood. 1991. "Molecular genetic analysis reveals a putative
`bifunctional polyketide cyclase/dehydrase gene from Streptomyces coelicolor and Streptomyces
`violaceoruber, and a cyclase/O-methyltransferase from Streptomyces glaucesens," Tetrahedron,
`47:6029-6043.
`
`
`17. Arrowsmith, T.J., F. Malpartida, D.H. Sherman, D.A. Hopwood, A. Birch, J.A. Robinson. 1992.
`"Characterization of actI-homologous DNA encoding polyketide synthase genes from the monensin
`producer Streptomyces cinnamonensis." Mol. Gen. Genet. 234:254-264. PMID:1508151
`
`
`18. Sherman, D.H., Eung-Soo Kim, M.J. Bibb, and D.A. Hopwood, 1992. "Functional replacement of
`polyketide synthase genes in Streptomyces coelicolor by heterologous genes from a different
`polyketide pathway," J. Bacteriol. 174:6184-6190. PMCID: PMC207686
`
`
`19. Malmberg, Li-Hong, Sherman, D.H., Hu, Wei-Shou, 1993. "Analysis of rate-limiting reactions in
`cephalosporin biosynthesis," Annals of the New York Academy of Sciences 665:16-26.
`PMID:1416602
`
`
`20. Khosla, C., McDaniel, R., Ebert-Khosla, S., Torres, R., Sherman, D.H., Bibb, M.J. and Hopwood,
`D.A., 1993. "Genetic construction and functional analysis of hybrid polyketide synthases containing
`heterologous acyl carrier protein." J. Bacteriol. 175:2197-2204. PMCID: PMC204504
`
`
`21. Malmberg, L.-H., Hu, W.-S. and D.H. Sherman, 1993. "Precursor flux control through targeted
`chromosomal insertion of the lysine -aminotransferase gene in cephamycin C biosynthesis." J.
`Bacteriol. 175:6916-6924. PMCID: PMC206817
`
`
`22. Hopwood, D.A., C. Khosla, D.H. Sherman, M.J. Bibb, S. Ebert-Khosla, E.-S. Kim, R. McDaniel,
`W.P. Revill, R. Torres, and T.-W. Yu. 1994. "Toward an understanding of the programming of
`aromatic polyketide synthases: a genetics-driven approach," in R.H. Baltz, G.D. Hegeman, and P.L.
`Skatrud (Eds.), Industrial Microorganisms: Basic and Applied Molecular Genetics, American
`Society for Microbiology, Washington, D.C.
`
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`23. Kim, E.-S., D.A. Hopwood and D.H. Sherman, 1994. "Analysis of type II polyketide ß-ketoacyl
`synthase specificity in Streptomyces coelicolor A3(2) by trans complementation of actinorhodin
`synthase mutants." J. Bacteriol. 176:1801-1804. PMCID: PMC205275
`
`
`24. Kim, E.-S., Bibb, M.J., Butler, M.J., Hopwood, D.A., and D.H. Sherman. 1994. "Sequences of the
`oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus." Gene
`141:141-142 (1994) PMID:8163168
`
`
`25. Bibb, M.J., D.H. Sherman, S. Omura and D.A. Hopwood. 1994. "Cloning, sequencing and deduced
`functions of a cluster of Streptomyces genes probably encoding for biosynthesis of the polyketide
`antibiotic frenolicin." Gene 142:31-39. PMID:8181754
`
`
`26. August, P.A., Flickinger, M.C. and D.H. Sherman. 1994. "Cloning and analysis of a locus (mcr)
`involved in mitomycin C resistance from Streptomyces lavendulae." J. Bacteriol. 176:4448-4454.
`PMCID: PMC205660
`
`
`27. Malmberg, L.H., A. Khetan, D.H. Sherman and W.-S. Hu. 1994. “Metabolic engineering of
`cephalosporin biosynthesis in Streptomyces clavuligerus.” In Advances in Bioprocess Engineering.
`E. Galindo & O.T. Ramirez, Eds.: 413-416. Kluwer Pub.
`
`
`28. Kim, E.-S., Cramer, K., Shreve, A., and D.H. Sherman. 1995. "Heterologous expression of an
`engineered biosynthetic pathway: functional dissection of type II polyketide synthase components
`in Streptomyces species.” J. Bacteriol. 177:1202-1207. PMCID: PMC176724
`
`
`29. Crosby, J., Sherman, D.H., Bibb, M.J., Revill, W.P., Hopwood, D.A., and T.J. Simpson, 1995.
`"Polyketide synthase acyl carrier proteins from Streptomyces: Expression in Escherichia coli,
`purification and partial characterization." Biochimica et Biophysica Acta 1251:32-41.
`PMID:7647090
`
`
`30. Malmberg, L.-H., Hu, W.-S., and D.H. Sherman. 1995. “Effects of enhanced lysine -
`aminotransferase activity on cephamycin C biosynthesis in Streptomyces clavuligerus.” Applied
`Microbiol. Biotechnol 44: 198-205. PMID:8579831
`
`
`31. Khetan, A., L.-H. Malmberg, D.H. Sherman and W.-S. Hu. 1995. “Metabolic engineering of
`cephalosporin biosynthesis in Streptomyces clavuligerus.” Annals of the New York Academy of
`Sciences 782:17-24. PMID:8659893
`
`
`32. August, P.R., J.A. Rahn, M.C. Flickinger, and D.H. Sherman, 1996. “Inducible expression of the
`mitomycin C resistance gene product (MCRA) from Streptomyces lavendulae.” Gene 175:261 - 267.
`PMID:8917108
`
`
`33. Williams, M.D., A.M. Fieno, R.A. Grant and D.H. Sherman. 1996. “Expression and analysis of a
`bacterial poly(hydroxyalkanoate) synthase in insect cells using a baculovirus system.” Prot. Exp.
`Purif. 7:203-211. PMID:8812862
`
`
`34. Williams, M.D., J. Rahn, and D.H. Sherman. 1996. “Production of a polyhydroxyalkanoate
`biopolymer in insect cells with a modified eucaryotic fatty acid synthase.” Appl. Environ. Microbiol.
`62:2540-2546. PMCID: PMC168036
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`35. Sheldon, P.J., D.A. Johnson, P.R. August, H.-w. Liu and D.H. Sherman. 1997. “Characterization of
`a mitomycin resistance determinant (mrd) from the producing organism. Streptomyces lavendulae.”
`J. Bact. 179:1796 - 1804. PMCID: PMC178896
`
`
`36. Johnson, D., P.R. August, C. Shackleton, H.-w. Liu and D. H. Sherman. 1997. “Microbial resistance
`to mitomycins involves a redox relay mechanism.” J. Amer. Chem. Soc. 119:2576 - 2577.
`
`
`37. Sheldon, P.J., D. A. Johnson, P.R. August, H.-w. Liu and D. H. Sherman. 1997. “Cellular self-
`protection against the antitumor antibiotic mitomycin C in Streptomyces lavendulae.” Industrial
`Microorganisms: Basic and Applied Molecular Genetics (R. Baltz, G. Hegemann and P. Skatrud),
`Vol. 34, pp. 123 - 130.
`
`
`38. Van Pilsum, J.F., D.H. Sherman, T.V. Line, A. Bedekar and L. Ayala. 1997. “Sequence comparison
`and functional analysis of amidinotransferases from eukaryotes and prokaryotes.” In De Deyn, P.P.,
`Marescau, B., Qureshi, I.A., and Mori, A. (eds.) Guanidino Compounds in Biology and Medicine II,
`London: John Libbey and Company LTD; 111 - 120.
`
`
`39. Bedeker, B., R.M. Zink, D. H. Sherman, T.V. Line, and J. Van Pilsum. 1998. “The comparative
`amino acid sequences, substrate specificities and gene or cDNA nucleotide sequences of some
`procaryotic and eucaryotic amidinotransferases: implications for evolution.” Comp. Biochem.
`Physiol. 119:677-690. PMID:9787760
`
`
`40. Zhao, L., D. H. Sherman, and H.-w. Liu. 1998. “Biosynthesis of desosamine: molecular evidence
`suggesting ß-glucosylation as a self-resistance mechanism in methymycin/neomethymycin
`producing strain, Streptomyces venezuelae.” J. Amer. Chem. Soc. 120:9374-9375.
`
`
`41. Zhao, L., D. H. Sherman, and H.-w. Liu. 1998. “Biosynthesis of desosamine: Construction of a new
`methymycin/neomethymycin analog by deletion of a desosamine biosynthetic gene.” J. Amer.
`Chem. Soc. 120:10256-10257.
`
`
`42. Xue, Y., L. Zhao, H.-w. Liu and D. H. Sherman. 1998. “A gene cluster for macrolide antibiotic
`biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity.” Proc. Natl. Acad.
`Sci. USA 95:12111-12116. PMCID: PMC22793
`
`
`43. Xue, Y., D. Wilson, L. Zhao, H.-w. Liu, and D. H. Sherman. 1998. Hydroxylation of macrolactones
`YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces
`venezuelae. Chem. & Biol. 5:661-667. PMID:9831532
`
`
`44. Zhao, L., N.L.S. Que, Y. Xue, D. H. Sherman, and H.-w. Liu. 1998. “Mechanistic studies of
`desosamine biosynthesis: C-4 deoxygenation precedes C-3 transamination.” J. Amer. Chem. Soc.
`120:12159-12160.
`
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`45. Borisova, S., L. Zhao, D.H. Sherman and H.-w. Liu. 1999. Biosynthesis of desosamine: Construction
`of a new macrolide carrying a genetically designed sugar moiety. Organic Lett. 1:133-136.
`PMID:10822548
`
`
`46. Zhao, L., J. Ahlert, Y. Xue, Jon S. Thorson, David H. Sherman, and Hung-wen Liu. 1999.
`Engineering A Methymycin/Pikromycin-Calicheamicin Hybrid: Construction of Two New
`Macrolides Carrying a Designed Sugar Moiety. J. Amer. Chem. Soc. 121:9881-9882.
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`47. Mao, Y., M. Varoglu and D.H. Sherman. 1999. “Molecular characterization of two genes (mitAB)
`required for biosynthesis of the antitumor antibiotic mitomycin C.” J. Bacteriol. 181:2199-2208.
`PMCID: PMC93634
`
`48. Sheldon, P.J., Y. Mao, Min He and D.H. Sherman. 1999. “Mitomycin resistance in Streptomyces
`lavendulae includes a novel drug-binding protein-dependent export system.” J. Bacteriol. 181:2507-
`2512. PMCID: PMC93678
`
`49. Mao, Y., M. Varoglu and D. H. Sherman. 1999. Molecular characterization and analysis of the
`biosynthetic gene cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae
`NRRL 2564. Chem. & Biol. 6:251-263. PMID:10099135
`
`50. Khetan, A., L.-H. Malmberg, Y. S. Kyung, D.H. Sherman and W.-S. Hu. 1999. “Precursor and
`cofactor as a check valve for cephamycin biosynthesis in Streptomyces clavuligerus.” Biotechnology
`Progress 15:1020-1027. PMID:10585184
`
`51. Han, Lei, A. Khetan, W.-S. Hu, and D.H. Sherman. 1999. “Time-lapse microscopy reveals temporal
`and spatial expression of lysine--aminotransferase gene in Streptomyces clavuligerus.” Mol.
`Microbiol. 34:878-886. PMID:10594815
`
`52. Belcourt, M.F., P.G. Penketh, W.F. Hodnick, D.A. Johnson, D.H. Sherman, S. Rockwell and A.C.
`Sartorelli. 1999. “Mitomycin resistance in mammalian cells expressing the bacterial mitomycin C
`resistance protein MCRA.” Proc. Natl. Acad. Sci. USA 96:10489-10494. PMCID: PMC17916
`
`53. Xue, Y., and D.H. Sherman. 2000. Alternative modular polyketide synthase expression controls
`macrolactone structure. Nature 403:571-575. PMID:10676969
`
`54. Xue, Y., D. Wilson, and D.H. Sherman. 2000. “Genetic architecture of the polyketide synthases for
`methymycin and pikromycin series macrolides.” Gene 245:203-211. PMID:10713461
`
`55. He, M., M. Varoglu and D.H. Sherman. 2000. “Functional analysis of conserved amino acid residues
`in the actinorhodin -ketoacyl-ACP-synthase.” J. Bacteriol. 182:2619-2623. PMCID: PMC111329
`
`56. Chen, S. Y. Xue, D.H. Sherman, and K.A. Reynolds. 2000. “Mechanisms of molecular recognition
`in the pikromycin polyketide synthase.” Chemistry & Biology 7:907-918. PMID:11137814
`
`57. Khetan, A., W.-S. Hu, and D.H. Sherman. 2000. “Temporal and spatial distribution of lysine--
`aminotransferase in Streptomyces clavuligerus.” Microbiology 146:1869 – 1880.
`
`58. Kyung, Yung Sun, W.-S. Hu and D.H. Sherman. 2001. Analysis of temporal and spatial expression
`of the CcaR regulatory element in the cephamycin C biosynthetic pathway using green fluorescent
`protein. Mol. Microbiol. 40: 530-541. PMID:11359561
`
`59. Kyung, Y. S., D. H. Sherman and W.-S. Hu. 2001. Simultaneous analysis of spatio-temporal gene
`expression for cephamycin biosynthesis in Streptomyces clavuligerus. Biotechnol. Prog. 17: 1000-
`1007. PMID:11735432
`
`60. He, M., P. Sheldon and D. H. Sherman. 2001. “Characterization of a novel quinone reductase
`activity for the mitomycin C binding protein (MRD): functional switching from a drug
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`activating enzyme to a drug-binding protein. Proc. Natl. Acad. Sci. USA 98:926–931. PMCID:
`PMC14686
`
`
`61. Varoglu, M., Y. Mao and D. H. Sherman. 2001. Mapping the mitomycin biosynthetic pathway
`by functional analysis of the MitM aziridine N-methyltransferase. J. Amer. Chem. Soc.
`123:6712-6713. PMID:11439066
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`62. Penketh, P. G., W. F. Hodnick, M. F. Belcourt, K. Shyam, D. H. Sherman, and A. C. Sartorelli.
`2001. Inhibition of DNA Cross-linking by Mitomycin C by Peroxidase Mediated Oxidation of
`Mitomycin C Hydroquinone. J. Biol. Chem. 276:34445-34452. PMID:11457837
`
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`63. Xue, Y. and D. H. Sherman. 2001. Biosynthesis and combinatorial biosynthesis of pikromycin-
`related macrolides in Streptomyces venezuelae. Metabolic Engineering 3: 15-26. PMID:11162229
`
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`64. Chen, S., J. B. Roberts, D. H. Sherman and K. A. Reynolds. 2001. The S. venezuelae pikAV gene
`contains a transcription unit essential for expression of enzymes involved in glycosylation of
`narbonolide and 10-deoxymethynolide. Gene 263: 255-263. PMID:11223265
`
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`65. Wilson, D.J., Y. Xue, K. A. Reynolds and D. H. Sherman. 2001. Characterization and analysis of
`the PikD regulatory factor in the pikromycin biosynthetic pathway of Streptomyces venezuelae. J.
`Bacteriol. 183:3468-3475. PMCID:PMC99645
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`66. Sang-Jung Kim, Han-Young Kang, and David H. Sherman. 2001. Synthesis of triketide delta-
`lactones. Synthesis 12:1790-1793.
`
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`67. Zhang, Q. and D. H. Sherman. 2001. Isolation and structure determination of novamethymycin, a
`new bioactive metabolite of the methymycin biosynthetic pathway in Streptomyces venezuelae. J.
`Nat. Prod. 64:1447-1450. PMID:11720530
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`68. Baumann, R. P., W. F. Hodnick, H. A. Seow, M. F. Belcourt, S. Rockwell, D. H. Sherman and A.
`C. Sartorelli. 2001. Reversal of mitomycin C resistance by overexpression of bioreductive enzymes
`in Chinese hamster ovary cells. Cancer Research 61:7770-7776. PMID:11691791
`
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`69. Baumann, R.P., D. H. Sherman and A. C. Sartorelli. 2002. A novel selection marker for mammalian
`cell transfection. BioTechniques 32:1030-1036.
`
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`70. Yoon, Y.J., Beck, B. J., Kim, B.S., Kang, H.-Y., Reynolds, K. A. and David H. Sherman. 2002.
`Generation of multiple bioactive macrolides by hybrid polyketide synthases in Streptomyces
`venezuelae. Chem. Biol. 9:203-214. PMID:11880035
`
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`71. Beck, B. J., Yoon, Y.J., Reynolds, K.A. and David H. Sherman. 2002. The hidden steps of domain
`skipping: mechanistic studies of macrolactone ring size determination in the pikromycin modular
`polyketide synthase. Chem. Biol. 9:575-583. PMID:12031664
`
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`72. Kim, B.S., T. A. Cropp, G. Florova, Y. Lindsay, D. H. Sherman, and K. A. Reynolds. 2002. An
`unexpected interaction between the modular polyketide synthases, erythromycin DEBS1 and
`pikromycin PikAIV, leads to efficient triketide lactone synthesis. Biochemistry 41:10827-10833.
`PMID:12196022
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`73. Cropp, T.A., B.S. Kim, B.J. Beck, Y.J. Yoon, D.H. Sherman and K.A. Reynolds. 2002. Recent
`developments in the production of novel polyketides by combinatorial biosynthesis. In
`Biotechnology and Genetic Engineering Reviews Vol. 19, pp. 159-172. Intercept Ltd. Andover, UK
`PMID:12520877
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`74. Chang, Z., P. Flatt, W. H. Gerwick, V.-A. Nguyen, C. L. Willis and D. H. Sherman. 2002. The
`barbamide biosynthetic gene cluster: a novel marine cyanobacterial system of mixed PKS-NRPS
`origin involving an unusual trichloroleucyl starter unit. Gene 296:235-247. PMID:12383521
`
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`75. Kim, B. S., T. A. Cropp, B.J. Beck, D.H. Sherman and K.A. Reynolds. 2002. Biochemical evidence
`for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin. J. Biol. Chem.
`277:48028-48034. PMID:12368286
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`76. Martin TW, Dauter Z, Devedjiev Y, Sheffield P, Jelen F, He M, Sherman DH, Otlewski J,
`Derewenda ZS, Derewenda U. 2002. Molecular basis of mitomycin C resistance in Streptomyces:
`structure and function of the MRD protein. Structure (Camb). 2002 10(7):933-42. PMID:12121648
`
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`77. Sherman, D.H. 2002. New enzymes for “warheads”. Nature Biotechnol. 20: 984-985.
`PMID:12355111
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`78. Podust, L.M., Kim, Y., Arase, M., Neely, B.A., Beck, B.B., Bach, H., Sherman, D.H., Lamb, D.C.,
`Kelly, S.C., and Waterman, M.R. 2003. The 1.92A structure of the Streptomyces coelicolor A3(2)
`CYP154C1: A new monooxygenase that functionalizes macrolide ring systems. J. Biol. Chem. 278:
`12214-12221. PMID:12519772
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`79. Beck, B.J., Aldrich, C.C., Fecik, R.A., Reynolds, K.A. and D. H. Sherman. 2003. Iterative chain
`elongation by a pikromycin monomodular polyketide synthase. J. Amer. Chem. Soc. 125:4682-4683.
`PMID:12696866
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`80. Beck, B.J., Aldrich, C.C., Fecik, R.A., Reynolds, K.A. and D. H. Sherman. 2003. Substrate
`recognition and channeling activities of mono-modules from the pikromycin polyketide synthase. J.
`Amer. Chem. Soc. 125:12551-12557. PMID:14531700
`
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`81. Podust L. M., Bach, H., Kim, Y., Lamb, D. C, Arase, M., Sherman, D.H., Kelly, S.L., and M. R.
`Waterman. 2004. Comparison of the 1.85 A structure of CYP154A1 from Streptomyces coelicolor
`A3(2) with the closely related CYP154C1 and CYPs from antibiotic biosynthetic pathways. Protein
`Sci. 2004. 13:255-268. PMCID: PMC2286509
`
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`82. Salomon, C. E., N. A. Magarvey and D. H. Sherman. 2004. Merging the potential of microbial
`genetics with biological and chemical diversity: An even brighter future for marine natural product
`drug discovery. Natural Product Reports 21:105-121. PMID:15039838
`
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`83. Kim, B.-S., Sherman, D. H. and K. A. Reynolds. 2004. An efficient method for creation and
`functional analysis of libraries of hybrid type I polyketide synthases. Prot. Eng. Des. Sel. 17:277-
`284. PMID:15067106
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`84. Srinivasan, A., Bach, H., Sherman, D. H. and J. S. Dordick. 2004. Bacterial P450-catalyzed
`polyketide hydroxylation on a microfluidic platform. Biotechnol. Bioeng. 88(4):528-535.
`PMID:15459906
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`85. Chang, Z., Sitachitta, N., Rossi, J.V., Roberts, M.A., Flatt, P.M., Jia, J., Sherman, D.H., W. H.
`Gerwick. 2004. Biosynthetic pathway and gene cluster analysis of curacin A, an anti-tubulin natural
`product from the tropical marine cyanobacterium Lyngbya majuscula. J. Nat. Prod. 67:1356-1367.
`PMID:15332855
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`86. Magarvey, N.A., Keller J.M., Bernan, V., Dworkin, M., and David H. Sherman. 2004. Isolation and
`characterization of novel marine-derived actinomycete taxa rich in secondary metabolites. Appl.
`Env. Microbiol. 70(12):7520-7529. PMCID: PMC535209
`
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`87. Hildebrand, M., Waggoner, L.E., Liu, H., Sudek, S., Allen, S., Anderson, C., Sherman, D.H., and
`M. Haygood. 2004. bryA: an unusual modular polyketide synthase gene from the uncultivated
`bacterial symbiont of the marine bryozoan Bugula neritina. Chem. Biol. 11(11):1543-1552.
`PMID:15556005
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`88. Jeong, J.-C., Srinivasan, A., Grüschow, S., Bach, H., Sherman, D.H., and J.S. Dordick. 2005.
`Exploiting the reaction flexibility of a type III polyketide synthase through in vitro pathway
`manipulation. J. Amer. Chem. Soc. 127(1):64-65. PMID:15631450
`
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`89. Nishizawa, T., Aldrich, C.C. and D. H. Sherman. 2005. Molecular analysis of the rebeccamycin L-
`amino acid oxidase from Lechevalieria aerocolonigenes ATCC 39243. J. Bacteriol. 187:2084-2092.
`PMCID:PMC1064027
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`90. Fortman J.L., Sherman D. H. 2005. Utilizing the power of microbial genetics to bridge the gap
`between the promise and the application of marine natural products. ChemBioChem. 6:960-978.
`PMID:15880675
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`91. Aldrich, C. C., Beck, B. J., Fecik, R.A. and D. H. Sherman. 2005. Biochemical investigation of
`pikromycin biosynthesis employing native penta- and hexaketide chain elongation intermediates. J.
`Amer. Chem. Soc. 127: 8441-8452. PMID:15941278
`
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`92. Aldrich, C. C., Venkatraman L., Sherman, D. H. and R. A. Fecik. 2005. Chemoenzymatic synthesis
`of the polyketide macrolactone 10-deoxymethynolide. J. Amer. Chem. Soc. 127:8910-8911.
`PMID:15969542
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`93. Venkatraman L., Aldrich, C. C., Sherman, D. H. and R. A. Fecik. 2005. Formal total synthesis of
`the polyketide macrolactone narbonolide. J. Org. Chem. 70:7267-7272. [Designated one of 20 Most-
`Accessed Articles during July-September 2005] PMID:16122247
`
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`94. Lee, S. K., Basnet, D. B., Hong, J. S. J., Jung, W. S., Choi, C. Y., Lee, H. C., Sohng, J. K., Ryu, K.
`G., Kim, D. J., Ahn, J. S., Kim, B. S., Oh, H. C., Sherman, D. H., Yoon Y. J. 2005. Structural
`diversification of macrolactones by substrate-flexible cytochrome P450 monooxygenases. Adv.
`Synth. Catal. 347:1369-1378.
`
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`95. Sherman, D. H. 2005. The Lego-ization of polyketide biosynthesis. Nature Biotechnol. 23:1083-
`1084. PMID:16151397
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`96. Beck, Z.Q., Aldrich, C.C., Magarvey, N.A, Georg, G.I. and D.H. Sherman. 2005. Chemoenzymatic
`synthesis of cryptophycin/arenastatin natural products. Biochemistry 44:13457-13466. [Designated
`“Hot” article for Biochemistry, November 2005] PMID:16216069
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`97. Mehra S., Lian W., Jayapal K.P., Charaniya S.P., Sherman D.H., Hu W.S. 2006. A framework to
`analyze multiple time series data: A case study with Streptomyces coelicolor. J Ind Microbiol
`Biotechnol 33(2): 159-172. PMID:16217633
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`98.
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`Beck, Z.Q. and Sherman, D.H. 2006. Development of an internally quenched fluorogenic substrate
`for analysis of thioesterases. Anal. Biochem. 349(2):309-311. PMID:16360105
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`99. Nishizawa, T., Grüschow, S., Jayamaha, D.E., Nishizawa-Harada, C. and Sherman,