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Mass (ug)
`
`Mass (ng)
`
`0.005
`0.005
`
`0.1
`0.1
`0.5
`0.5
`
`0.05
`
`5
`5
`
`100
`100
`500
`500
`
`Mass (pg)
`
`HGE
`1666.666667
`1666.666667
`
`Base pairs
`5.33333E+12
`5.33333E+12
`
`Fragment length (bp) No. of molecules
`136
`39,215,686,274.51
`
`186
`28673835125
`
`Moles
`6.51207E-14
`4.76151E-14
`
`5000
`5000
`
`1.00E+05
`1.00E+05
`5.00E+05
`5.00E+05
`
`33333.33333
`33333.33333
`166666.6667
`166666.6667
`
`1.06667E+14
`1.06667E+14
`5.33333E+14
`5.33333E+14
`
`50
`
`
`
`50,000
`
`
`
`16,667
`
`5.33333E+13
`
`700
`1330
`700
`1330
`
`200
`
`1.52381E+11
`80200501253
`7.61905E+11
`4.01003E+11
`
`2.5304E-13
`1.33179E-13
`1.2652E-12
`6.65896E-13
`
`2.66667E+11
`
`4.42821E-13
`
`Target DNA
`Nieuwerburgh
`Nieuwerburgh
`
`Meyer 2008
`Meyer 2008
`Meyer 2008
`Meyer 2008
`
`Meyer 2010
`
`Adapters
`Nieuwerburgh
`Meyer 2008
`Meyer 2010
`
`Volume (liter)
`
`1.00E-06
`2.00E-06
`0.0000002
`
`Concentration (uM) Molar concentration
`15
`0.000015
`500
`0.0005
`200
`0.0002
`
`Moles
`
`1.50E-11
`1.00E-09
`4.00E-11
`
`pmol
`15.00
`
`1,000.00
`
`40.00
`
`
`136 bp fragment
`186 bp fragment
`
`700bp fragment
`1330 bp fragment
`700bp fragment
`1330 bp fragment
`
`Reference
`Nieuwerburgh
`Nieuwerburgh
`
`Meyer 2008 (100 ng)
`Meyer 2008 (100 ng)
`Meyer 2008 (500 ng)
`Meyer 2008 (500 ng)
`
`200 bp fragment
`
`Meyer 2010
`
`Adapter (moles)
`1.50E-11
`1.50E-11
`
`Target DNA (moles)
`6.51207E-14
`4.76151E-14
`
`Adapter:DNA
`230.34
`
`
`315.03
`
`1.00E-09
`1.00E-09
`1.00E-09
`1.00E-09
`
`4.00E-11
`
`2.5304E-13
`1.33179E-13
`1.2652E-12
`6.65896E-13
`
`3,952
`
`
`7,509
`790
`
`
`1,502
`
`4.42821E-13
`
`
`
`90.33
`
`00001
`
`EX1075
`
`

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