CA 02251265 1998-10-21
`
`Table 10: Fatty acid composition E. pacifica
`Solvent
`Saturated
`Unsaturated
`Mono
`22,54
`22,18
`22,11
`22,96
`22,98
`
`chio-meth
`acetone
`acetone
`ethanol
`
`26,18
`21,4
`19,09
`45,93
`45,96
`
`Di
`1,91
`1,75
`=2,03
`1,23
`1,24
`
`Unidentified
`
`Poly
`4,31
`4,67
`4,79
`2,72
`2,48
`
`H-Poly
`26,34
`24,52
`30,24
`11,11
`11,18
`
`18,72
`25,49
`21,72
`16,05 (500 pg/mL)
`16,15 (200 pg/mL)
`
`Data expressed in percentage of total fatty acids (%).
`
`\3
`
`0000020
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0451
`
`page 0451
`
`

`

`CA 02251265 1998-10-21
`
`TABLE 11. OPTIMAL CONDITIONS FORLIPID EXTRACTION OF
`AQUATIC ANIMAL TISSUES(suggested procedure)
`
`
`STEP
`
`CONDITIONS
`
`Grinding(if particles > 5mm)
`
`4°C
`
`Lipid extraction
`
`Filtration
`
`Washing
`
`Filtration
`
`Evaporation
`
`Oil-water separation
`
`Lipid extraction
`
`Filtration
`
`Evaporation
`
`sample-acetone ratio of 1:6 (w/v)
`2h (including swirling 20 min)
`4°C
`
`organic solvent resistantfilter
`under reduced pressure
`
`sample-acetone ratio of 1:2 (w/v)
`pure and cold acetone
`
`organic solvent resistantfilter
`under reduced pressure
`
`under reduced pressure
`
`4°C
`
`sample-ethano! ratio of 1:2 (w/v)
`pure ethanol
`30 min
`4°C
`
`organic solvent resistantfilter
`under reduced pressure
`
`.
`
`under reduced pressure
`
`20
`
`0000021
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0452
`
`page 0452
`
`

`

`CA 02251265 1998-10-21
`
`Bibliography
`
`Bowyer, D.E., Leat, W.M.F., Howard, A.N. and Gresham, G.A. 1962. The
`determination of the fatty acid composition of serum lipids separated by
`thin-layer chromatography; and a comparison with column chromatogra-
`phy. BBA. 70: 423-431
`
`Chandrasekar, B., Troyer, D.A., Venkatraman, J.T. and Fernandes, G. 1996.
`Tissue specific regulation of transforming growth factor beta by omega-3
`lipid-rich krill oi! in autoimmune murine lupus. Nutr Res. 16(3): 489-503
`
`Christensen, M.S., Hoy, C-E. and Redgrave, T.G. 1994. Lymphatic absorption
`of n-3 polyunsaturated fatty acids from marineoils with different intramole-
`cularfatty acid distributions. BBA. 1215: 198-204
`
`Difco laboratories. 1984. Difco Manual Dehydrated Culture Media and
`Reagents for Microbiology. 10™ ed. Detroit.
`
`Foich, J., Lees, M. and Sloane-Stanley, G.H. 1957. A simple method for the
`isolation and purification of totallipids from animaltissues. J. biol. Chem.
`226: 497-509
`
`Goodman Gilman, A., Goodman, L.L. and Gilman, A. 1980. The Pharmacological
`Basis of Therapeutics. 6" ed. Collier Macmillan Canada itd, Toronto.
`
`Heligren, L., Karistam, B., Mohr, V. and Vincent, J. 1991. Krill enzymes. A
`new concept forefficient debridement of necrotic ulcers. Int J Dermatol.
`30(2): 102-103
`
`Prawn Hatchery Food. 1997. http://Awww.kk-tech.com/Iillhtml
`
`Runge, J.A. and Joly, P. 1994. Rapport sur l'état des invertébrés en 1994-
`7:0 Zooplancton (Euphausiacés et Calanus) de|'Estuaire et du Golfe
`du Saint-Laurent.
`
`Sargent, J.R. 1997. Fish oils and humandiet. Br J Nutr.78 Supp! 1: S5-S13
`
`ZI
`
`0000022
`
`RIMFROST EXHIBIT 1024 page 0453
`
`RIMFROST EXHIBIT 1024
`
`
`page 0453
`
`

`

`CA 02251265 1998-10-21
`
`Although the present invention has been described herein
`
`above by way of preferred embodiments thereof, it can be modified, without
`
`departing from the spirit and nature of the subject invention as defined in the
`
`appendedclaims.
`
`0000023
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0454
`
`page 0454
`
`

`

`CA 02251265 1998-10-21
`
`WHAT.IS CLAIMED IS:
`
`1.
`
`A method for extracting lipids from an aquatic animal tissue
`
`comprising the stepsof:
`
`a) suspending said animal aquatic tissue in an organic solvent;
`
`b) extracting lipids by successive organic solvent treatment;
`
`and
`
`c)
`
`collecting said lipids in a first fraction and an organic
`
`insoluble fraction.
`
`2.
`
`The method of claim 1, wherein said organic solventof a)is
`
`acetone.
`
`3.
`
`The method of claim 1 or 2, wherein said organic solvent of b)
`
`is selected from at least one of acetone and alcohol.
`
`4.
`
`The method of claim 1, 2 or 3, wherein said organic insoluble
`
`fraction comprises a dry residue fraction which is enriched in protein.
`
`5.
`
`The method of claim 1, 2, 3 or 4, wherein said aquatic animal
`
`tissue is at least one tissue selected from the group consisting ofkrill tissue,
`
`Calanustissue andfish tissue.
`
`6.
`
`A lipid extract obtained by the methodofclaim 2, 3, 4 or 5.
`
`22
`
`0000024
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0455
`
`page 0455
`
`

`

`CA 02251265 1998-10-21
`
`7.
`
`8.
`
`A protein rich fraction obtained by the method of claim 4 or5.
`
`A lipid extract having the properties in accordance with the
`
`presentinvention.
`
`23
`
`0000025
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0456
`
`page 0456
`
`

`

`CA AAA TIAT 1998-10-21
`
`: 98-03-24 20:09:39
`Injection Date
`Sample Name a
`Acq. Operator
`: Chantal Beaudoin
`
`-
`Seq. Line :
`1
`Vial
`:
`1
`Inj
`:
`In} Volume : Manually
`
`Method
`Last changed
`
`|
`i
`|
`
`°
`5
`wo
`
`40 -
`-
`
`b
`
`<
`a
`
`wa
`0
`a
`~~
`-
`
`i
`
`{
`
`:
`|
`
`a
`
`:
`:
`i
`
`«
`|
`lewo
`l= =
`a0
`= |
`
`—_844..™ °Ls
`
`: C:\HPCHEM\1\METHODS\ALAIN2 .M
`: 98-03-24 19:56:07
`by Chantal Beaudoin
`(modified after loading)
`Méthode corrigée lors de l'installation de la nouvelle colonne 12 septembre
`1997. Température du four 170 degré C et purge flow = 150 ml/min. Flux dans
`la colonne : 4,0 ml/min. Augmentation de la température a 175 degré C et
`le
`purge flow est descendu a 140 ml/min,
`le 13 mars 1998.
`.
`
`FID1 A, of GEN00002.0
`i
`<3
`oo
`PAT
`Rae
`&
`| b
`|
`2
`:
`
`la
`|
`55 —
`i
`.
`i
`=
`
`;
`50-
`i
`6
`
`[=22.606-20:4(6,10,14,18)
`
`tTaPOFi,.
`¥:oe
`HADetebesetbeethawtedeeabanabatdaa bsBoteoO8
`
`
`Wet.BEciety
`62.225
`19.344-20:2
`zon5G5.876
`
`an-_
`
`—_—_
`
`>26.247
`D>40.655
`)}56.373
`MA
`
`
`ow
`oY
`ny
`AeTicea-t
`ie3Pe
`YLa
`
`24.103
`
`31.295
`
`49.721
`
`>°o
`
`3
`
`Figure 1: Gas-liquid chromatography of fatty acids from dry krill (chloroform-
`methanol).
`
`CONFIDENTIEL
`
`
`
`0000026
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0457
`
`page 0457
`
`

`

`.
`
`Injection Date
`Sample Name
`Acq. Operator
`
`
`CA 02251265 1998-10-21
`UNELUEN Lib
`: 98-03-25 20:00:46 -
`: 11
`: Chantal Beaudoin
`
`ao
`-
`Seq. Line :
`1
`Vial
`:
`1
`Inj
`:
`Inj Volume : Manually
`
`Method
`Last changed
`
`: C:\HPCHEM\1\METHODS\ALAIN2.M
`: 98-03-25 18:55:58
`by Chantal Beaudoin
`(modified after loading)
`Méthode corrigée lors de l'installation de la nouvelle colonne 12 septembre
`1997. Température du four 170 degré C et purge flow = 150 ml/min. Flux dans
`la colonne : 4,0 ml/min. Augmentation de la température a 175 degré C et
`le
`purge flow est descendu a 140 ml/min,
`le 13 mars 1998.
`FID1 A, of GENO0005.0
`
`a
`
`50
`
`=BAAD1st
`
`~~wT
`x
`
`xw
`
`
`|
`
`45
`40
`
`20
`
`ii ' | |I|
`
`3
`‘
`:
`
`o
`N
`t
`s
`|
`=
`35
`|
`2
`=
`|
`i
`os
`5
`x
`g
`|
`ie &§&
`FS
`|
`1
`lm *
`
`25
`fh
`~
`nw
`Ff
`5
`g. |!
`ff
`2
`Ae tee)
`8
`a
`A
`it —
`aM
`+
`7 art
`
`nO
`-
`
`\
`
`Figure 2: Gas-liquid chromatographyoffatty acids from dry krill (acetone).
`
`CONFIDENTIEL
`
`
`
`0000027
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0458
`
`page 0458
`
`

`

`CA 02251265 1998-10-21
`
`Injection Date
`Sample Name
`Acq. Operator
`
`: 98-04-01 18:48:05
`: 26
`: Chantal Beaudoin
`
`-
`Seq. Line :
`1
`Vial
`:
`1
`Inj
`:
`Inj Volume : Manually
`
`Method
`: C:\HPCHEM\1\METHODS\ALAIN2.M
`Last changed
`: 98-04-01 18:45:50
`by Chantal Beaudoin
`(modified after loading)
`Méthode corrigée lors de l'installation de la nouvelle colonne 12 septembre
`1997. Température du four 170 degré C et purge flow = 150 ml/min. Flux dans
`ta colonne : 4,0 ml/min. Augmentation de la température a 175 degré C et
`le
`2urge flow est descendu a 140 ml/min,
`
`12.140-18:3
`
`&
`
`
`
`24.071
`
`
`
`le 13 IMars 1998. FID1 A, of GEN00008.D
`en, 22.575-20:4(6,10,14,18)
`
`
`>-====62.250
`F>26.215 NSo
`
`
`
`Figure 3: Gas-liquid chromatographyoffatty acids from frozen krill (acetone).
`
`CONFIDENTIEL
`
`
`
`0000028
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0459
`
`page 0459
`
`

`

`tr,
`
`CA 02251265 peg lot
`
`Injection Date
`Sample Name
`Acq. Operator
`
`: 98-04-02 17:35:45
`: 28
`: Chantal Beaudoin
`
`-
`Seq. Line :
`1
`Vial
`:
`1
`Inj
`:
`In} Volume : Manually
`
`Method
`Last changed
`
`: C:\HPCHEM\1\METHODS\ALAIN2 .M
`>: 98-04-02 17:28:39
`by Chantal Beaudoin
`(modified after loading)
`Méthode corrigée lors de l'installation de la nouvelle colonne 12 septembre
`1997. Température du four 170 degré C et purge flow = 150 ml/min. Flux dans
`la colonne : 4,0 ml/min. Augmentation de la température a 175 degré C et
`le
`. purge flow est descendu a 140 ml/min,
`le 13 mars 1998.
`t
`FID? A, of GENO0009.D
`
`eoel48a
`
`oo
`™¢
`“=
`Oo=
`
`~
`-
`
`©
`1
`
`&§
`oan
`
`
`
`
` =~
`
`:
`.
`
`:
`:
`:
`
`'1
`
`¢net
`ns
`
`aa. ,
`9
`Sa
`mw
`=™
`N
`
`= A
`
`Figure 4: Gas-liquid chromatography of fatty acids from frozen krill (ethanol).
`
`CONFIDENTIEL
`
`
`
`0000029
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0460
`
`page 0460
`
`

`

`0000030
`
`— ss ya
`; of Calanus
`of
`neutral |
`ipi
`dsof
`SP:
`Si
`); Sample of other
`cholest
`Tol.
`nterest,
`e
`no.
`iga
`(acetone) and M. n
`icai
`orveg
`.
`C acid (90
`viv)
`10
`.
`
`oem wee
`
`‘‘c
`wn.‘a=raaa=<
`
`weaa-=
`
`1
`
`'
`
`==
`
`=a
`
`awane
`
`raeat
`
`eeraa
`
`santhye3aeh,
`
`payee
`
`arn
`ark
`
`°i
`
`i 1
`
`aceti
`
`~¢
`
`TENG
`
`wdhates
`
`rataTevt
`Hyra
`
`
`
`creesora
`
`as
`
`attaDeans
`
`ma
`
`
`
`Laeryeepe
`
`Prey
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0461
`
`page 0461
`
`

`

`0000031
`
`(a
`:
`ds of E
`ceton
`cifica
`ipi
`pa
`—_.
`ag(ace
`ackt
`one)
`cholesterol 20
`mg/mL, sample
`interest;Cai:
`(
`anus
`aceton
`e) and Calanus s
`Hexane-ethyl ethe
`F:
`d (90
`10:1
`viv)
`
`“>7)
`
`preaereres
`
`Te
`
`ee
`
`’ed
`
`erert
`
`
`
`eeretsSiedateeae
`
`iioe
`
`hosoaered
`
`seedobNedtwe
`
`e),
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0462
`
`page 0462
`
`

`

`Ss
`
`7 Parry ae
`
`NCOSerreSe
`
`eeRTOS
`
`=
`
`spt
`
`sp.
`ica
`
`(acetone);
`(acetone)
`
`0000032
`
`Soent ge
`hit3)
`ri
`raphy
`a85865
`fi
`chromatog
`Figure -7:-T!
`pids of
`ye
`EM
`Calanus s
`(
`ethanol)
`fm
`p:
`(ethanol) and egg (acetone).
`n
`norvegica
`vA).
`2
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0463
`
`page 0463
`
`

`

`0000033
`
`yhic
`ds ‘of. Calan
`sholip
`eeezio>
`nus S|
`0s
`SP.
`D.
`oles
`imL;-E-: paci
`esterol:20
`~s
`(
`tone)..
`egg (ace
`vN)
`22),
`)
`
`page 0464
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0464
`
`

`

`CA 02251265 1998-10-21
`
`8@
`
`9%
`
`vec02st91rlZlol89¥@
`
`
`
`NOLLOVULXC111NOANOLAOVNIAWLLNOLLV@NNIJOFONANTANI6TUN
`
`‘(Sjied|)
`
`
`
`
`
`*(y)auojaoNUyauNy}UONEQnoUy
`
`
`
`(%FUB)S62]WONTLIVA)£9,8I1]CpI)UYsUOTeUTALIDIIG
`
`
`
`*(A/M)62]JOOFFA2U0}298-9]dIES
`
`
`
`
`
`“(euseq sq819M Yea& UO “,) ppIy
`
`
`
`0000034
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0465
`
`page 0465
`
`

`

`
`
`
`
`NOLLOVULX4CIdI'INOINOLAOV40AWNTOAFALAOFONANTANI‘OLFUND
`
`CA 02251265 1998-10-21
`
`‘(aged“q)
`
`sz
`
`s‘I
`*(s1s¥q q8taM Ysa E UO ‘Y,) PRIA
`
`
`
`“GZjoaul)UOPEQnouU]
`
`ss
`
`ossyOvs¢oCSz0zsl
`
`...ol!bb:8:1b:19:1S:)m3=:
`.*(ryun)auojaoeJoaumnpoA
`
`
`
`
`*(%§UBY)$69]UORELIGA)sayRoqdps)OYsUOPEAyUII;0q
`
`
`
`
`
`0000035
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0466
`
`page 0466
`
`

`

`
`
`
`
`*(199881T)NOLLDVULXGAIdI'TNOIONVHLGNIQWNOLLV@NONIdOFONGNTANITTFUND
`
`CA 02251265 1998-10-21
`
`s‘Z
`
`*(stseq yy3rom Arp & U0 ‘ey,) PRL
`
`
`
`-(unu)jousyyauyaapTOREQn>UT
`
`
`
`“(ajm)921JOO18jounqy2-2jdureg
`
`Sel
`
`0z1
`
`sol06$l09SPofSI0
`
`
`
`(%§UID£83]VONELTEA)saNONdLYWy]sHONTUTULIAVIg
`
`
`
`
`
`0000036
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0467
`
`page 0467
`
`

`

`
`
`
`
`«(Gaoed“q)NOLLOVULXYidlNOTONVHLdJOAWQIOAABLJOFONANTANICTFANT
`
`CA 02251265 1998-10-21
`
`ee
`
`Ol
`
`“(siseq sy319m Aap & UO ‘%) PPA
`
`Szl
`
`
`+31gj(1m)tomesoume2tl
`00!SLosSZ
`(10a)UwyysJODWNoO.
`
`
`
`“uyurggJoaul)UOEQNIUT
`
`
`
`
`
`“(%§WEY)889]WOPEPIWA)sIwI_IMYA}UYsuo}wO]ULIZIAG
`
`
`
`
`
`
`
`0000037
`
`RIMFROST EXHIBIT 1024
`
`RIMFROST EXHIBIT 1024 page 0468
`
`page 0468
`
`

`

`®A+4H # F CIP)
`
`@ FF FF Hi BA 2 Ba’
`
`GBRMRRAR (AD
`
`H6O- 153779
`
`@Int ,Ct,4 @2308+§BRIG0ZE( 1985) 8 A138mazes TASHES
`
`
`A23L 1/42
`8412—4B
`.
`A 61K 9/48
`6742—4C
`31/355.
`7330—4C SEHR ik FRWHOM 1
`ADL
`OQRWOZA FMA
`
`
`
`(428)
`
`GOFF
`@Hi
`
`#A59— 10625
`RA Fas9(1984)1 24H
`f i RRPLEE 4 TAI BES
`@s% HH 2 #5
`@s Fe 8 fi
`SRETKIUL M4 M21M304
`On BAK BERMAKASH WRTRERAPH 1TA2638
`
`1. BHO
`oe HH Bh de
`
`2 WER ©
`Q eC F tyr peIenKevry sts rent aryrrres
`ZY PRSHLOMMC BML CLARAKK DEX
`SF YOASHAARCHAL AR Bm he
`Q TAINLY REY REBOAK MAB. 179% mH,
`Pet. Tat, tar eth, feo mmhEore
`RR th CD SMR OMB) ARMOR
`3% HE Bh fe dio
`
`A BHORM SMH
`KRAUL CS EY RL KEV Fe REE RT HR
`YA TY PRSROHMEERDL I SMM SD FH Re AB BD
`RMCBFZ’COCTHS,
`ME. ORO REB MCA OF RRA TY AOS,
`OUP EROREM MCR OTMR., AMR. OAM,
`KB. REORRMRH NF TAOC THEI DARMRMRBA,
`@QERLCHAK DBE LAS MMRROLGKREHE. BORR
`CRRELTCRKRAMDRHAORBOSA MIC MPme ce db.
`
`MCRAMHMREBOKMCL OTCHHHEALT ERM
`SRAOHEEADARAMTENCHRENTHS,
`
`ARHACOLIRREBED = -ADSHANKLO
`THO, OHMOKLEM SC. O2VAF U-— ABET
`CMR B eke. QM ROBE e ea
`TCMMOBAKE AT. OHPe Piel CHE PEO
`MAEM LF AGOMMEATSYZ or BL, DEB
`PYERGEHTAAY NY ATHY RMORME EMT So.
`QavzFr—-AEGFAYE COREE HLT SSO
`MRED SKEVYS YL, Mh RRR BS
`CHM, HOMAGE OMETAT S21 eK YZ
`ZY RAROKRVMEAREAMAARHEMM RAE
`He AEFA*OCHSA.
`,
`tedo>bob. ARBPRH, CSP YRSRLOK RY Y
`RA ayn yr SeYRMERMOAKMICBHELCZSEAA
`RRM EUM SF YOR TEND AICBA L tt HE HG BD & ch
`THs
`
`REALE MN CMA PSE REY EH, ARO MH.
`MAM. HWMORT + CMEBTFRASAWiUZ aw
`YS 7A- SCL OC RMT ADETCHSONAELOK
`MACHSM. CONMEKDRBANSZA~OTHAC,
`EH. COMMR’LRMBINAW.
`HREM 7 IT—- th. KM, eH reo RM
`wMmPprie sir EMEC RAN CHSH. COBH
`RAHN AZKWMFCHAAMOEHECTOR? BA
`FScCcEKHELC EH,
`
`HABACHPIT ACSI YREORG RM. AF ean WiC H
`
`| —363=
`
`000001
`
`AKBM 1074
`RIMFROST EXHIBIT 1024 page 0469
`RIMFROST EXHIRERBA7 Reso8d9S38
`
`

`

`AF SEKWEKPK HEHSHEODECLLIKGRE
`CHO. CHUPFCHHEA CORR BEHAOH DY
`MMOCLE LSS + EOMMMTHHENEM,
`BRL CRY RL OMT SKE Y eri, BBL
`ZMOMAATMUMETSEA ABR EMA, BMLCA.
`SHRAKTMERARARIY MM RARE Y FY
`MMe CH SM Teby. Te a-n SEL RB
`ERRRMAN RE SY EHTEL BRL
`TY EROPRVAELLCU TIT VN +e RREORKHHH
`VEFVLEMATSCERCTHES.
`COKRVYS YM, AF eEABRCHAS SMRKMSEH
`
`HK AO SMA S A Lei KBHTHD. COME
`THM RO CLEAR VY ST YOMMmBATHAASN AK,
`SGML EASY REKEUY FYE aMT RR
`Ayan yr & ( Cro:s) FRO BM Mi. 17 -Y Mh.XA
`Htho ae TT tthe Sv srMmMeoceaeazraAarr~y
`FLY RESKWEAATAKEMWMOM MM, BB
`
`VEEN SOMHSAMMOFRK LOTR HY
`ARYREMMLCHSHAMEHEMCAS,
`RBACHPHATDAWVY AY ee Y REBORN MOB
`ARM HFT RAAKCHAPFSMKHSKPK Hw Ss HH
`BMPR OLLIOKGECHO. ChuF CURT SZ
`YR ARTY ROAKA CTO +: MIKO DL oe
`OLLE EAT RY FTYROMHOTHRA NAD,
`ESI YERILEKR UY KF vRZ THN YAR
`
`TH 4g 60-153779(2)
`SRORVMCBAL CS SRAKKHE. BREE.
`CHEGKRHBORMRELCM RTE SOA AK SOT,
`AMEE. T2797 — Mh, Koh, ee th, ARM,
`RAMFORAMH MM CBRTSCL ATES.
`COLOR LCHENAREMKHM. ROC, BEE
`FE CTU SFY OAF eAnCHATS>
`COMAHKO—-HMELTM, HARKRHMEK ETSY,
`FURY, BPIUKE AMER MRBLA EFF A
`FRrACHMeREAL, FOR, ZANE MRBAET
`ABAOR MAR Re META.
`BFF YRS eA ORRARBL FFE -—K-ABSEE
`
`HCH.
`COLI LUN ONKRABH ORM HED RA. AUS
`DEIREMK, AUAF BO -AE TO. MMM AO
`BEEP AAL MEERUT ZSEOWnMAEAPT SUL Fe
`~BAL,
`CERF ST YRER EU TABBY NY AT YROKRM
`€RML, Uae a-nAe GP SH AROK MEAT
`BKB VSS VL. AMMAR L SAME LK
`MWRRLEORHLHTATIA SAY eae REBA
`FSZCOCH SHS, CHSEGHBEARAOHNE HB
`Cl et. MPT AF A-A EE. HE & RY
`MEHR ORCHET ERA, LHRH. BEBO
`CER MRHERAS. MRE RKA RO ihe 6U FH
`CMRF SH, MAME LCOS BALES
`LOUTHES.
`
`KRCKBHNORAMERT o
`6 FI 1.
`
`CAS YEQS MERMPLEKRV YS YY 25KE
`EMM Drm Otte eee A Ye 16
`
`S)SORMRCRAL. MEU TK mE, MELT
`EL
`“BH. ©9F > 6U RAM. TU sO RH,
`KYO RRMEB-KHRALL 7147 DRE LAER,
`SRO Z0InP OATH NMKCKBARML CXS HY
`BBtW Leo
`CORBCMEORAKKMERAL, LOS KH,
`KOECMWMEHLTKRA OH CHD Re Hr 6
`Re 2.
`CAS YR SU RRMPLOKRYY + + SOKRRHA
`&. BROT at AY key BC OBO fh Be KK
`Wl eras AL EAH AYr EY RAE 2G %
`EFI T7—-MeE lI -ORMMATHALA=1
`3X yr, eT YRAEROKRVY MIO MRRMCEMREL.,.
`
`EE
`
`HSV CKMR, RIF LCH-KBMLA.
`—H. eee + 6O BRB. FTV eK Y 50 RR. K
`10RRMEB-KAAL. FIX RELA K.
`BH 200mI OAS eEAKICHHMBALCLFIFtY RSE
`MBL.
`COBBSCMRORRRKHERAL. LeSR.
`ADE HR E HL CHK HAO Pe Hi he EHR.
`
`—364—
`
`000002
`
`RIMFROST EXHIBIT 1024 page 0470
`RIMFROST EXHIRIERBA7Re808bAS39
`
`

`

`(19) Japan Patent Office (JP)
`(12) Patent Gazette (A)
`(11) Japanese Patent Application Publication
`Number HEI 8 — 231391
`
`(51) Int. Cl 4 Classification Symbol Internal No.
`A23L 1/42
`8412 — 4B
`A61K 9/48
`6742 —4C
`31/355
`7330 —4C
`
`ADL
`
`(43) Publication Date August 13, 1985
`
`(54) Title of Invention
`
`(72) Inventor
`(72) Inventor
`(71) Applicant
`
`Request for Review Unrequested Number of Claims 1 (Total 2 Pages)
`Nutritional Supplement
`(21) Application Number
`Sho 59 — 10625
`(22) Application Date
`January 24, 1984
`Motuski Kenji
`Tokyo-to, Nakano-ku, UenoMiya 4 Choume 9 Ban 6 Go
`Fukuoka Ryuu
`Tama-shi Nagayama 4 No 4 No 21 No 304
`Honen Seiyu Co. Ltd.
`Tokyo-to, Chiyoda-ku, Otemachi 1 Choume 2 Ban 3 Go
`
`Specification
`1. Title of Invention
`Nutritional supplement
`2. Scope of Claims
`(1) A nutritional supplement being contained inside
`a gelatin capsule and comprising a nutritional liquid
`being formed by melting vitamin E, soybean
`lecithin in an oil having high eicosapentaenoic acid
`content.
`(2) A nutritional supplement of claim 1 wherein the
`oil having high eicosapentaenoic acid contentis a
`sea animaloil such as pilchard oil, mackerel oil,
`cuttlefish oil, krill oil, or mink oil.
`3. Detailed Explanation of the Invention
`The present invention relates to a new nutritional
`supplementhavingas its primary ingredients
`vitamin E, soybean lecithin, and an oil having high
`eicosapentaenoic acid content. In recent years, the
`demand for nutritional supplements has greatly
`increased due to factors such as (1) lack of
`nutritional balance brought about by a rich food
`culture, (2) pickiness and lack of food diversity
`causedby a selective food palette, (3) lack of
`nutrition brought about by a breakdownin balance
`of nutrition and health, exercise, and energy, and (4)
`the necessity for supplementary nutrition to
`correspond to an aging population. In particular, the
`desire to stem adult disease by the improvementof
`food life is strong, so people enjoy eating nutritional
`supplements.
`
`The present invention arises out of the
`aforementioned nutritional needs andits object is to
`provide a new nutritional supplement from the
`combination of vitamin E, which (1) prevents the
`aging of cells, (2) lowers cholesterol and prevents
`the hardeningofarteries, (3) prevents the
`occurrence of liquid fatty deposits and encourages
`cell life, (4) cleans and washesbloodvessels and
`prevents brain or heart blood clots; soybean lecithin,
`which (1) encourages the absorption of vitamin E
`and eicosapentaenoic acid, (2) reduces cholesterol
`and prevents hardening of blood vessels and oil
`having high eicosapentaenoic acid content, whichis
`effective anti-platelet aggregation, making it an
`anti-coagulant and acts to prevent the hardening of
`blood vessels.
`In other words, the present invention is a
`nutritional supplement consisting of vitamin E and
`soybeanlecithin melted in an oil having high
`eicosapentaenoic acid content in liquid form
`inserted into a gelatin capsule.
`
`Asfor the vitamin E used in the present invention,
`items obtained by publicly known methods such as
`concentration by chromatography or molecular
`distillation of plant oils are appropriate, but the
`method of production thereofis not limited, and the
`source of energy is also not limited.
`Vitamin E is included in great amounts in
`saffloweroil, rice oil, corn oil, and other oil from
`water dwelling plants. However, the concentration
`
`AKBM 1014
`RIMFROST EXHIBIT 1024 page 0471
`RIMFROST EXHIBIRAR847 |Pemsosb4540
`
`

`

`of the oil is at most 0.3%, so using any of these as-is is not desirable.
`
`‘The amount of vitamin E included in the present invention must be at
`least 1% of the liquid included in the capsule. If less than this
`concentration is achieved the activity of the vitamin in the body is
`reduced, and the benefits of vitamin E cannot be achieved.
`
`Also, soybean fatty substance containing soybeanoil obtained by
`dehydrating and drying gum produced by the production of soybean
`gumis generally acceptable for the soybeanlecithin used in
`conjunction with vitamin EF,butit is also acceptable to use lecithin
`that has been sugared and concentrated via acetone or alcohol, and it
`is also possible lo use separated lecithin that contains litle of no
`keratin.
`
`This soybean lecithin must be present in the liquid thatis inserted
`into the capsule in a concentration of at least 1%. As noted before,if
`the concentration falls bclow this level the effects of the substance
`will not be sufficient. In addition, for 011 with a high concentration of
`eicosapentaenoic acid (C20 : 5) that has vitamin E and soybean
`lecithin dissolved within it, an oil with a eicosapentaenoic acid
`content of 8% or higher may be used, such as that found in sea
`animaloils such as pilchard oil, mackerel oil, cuttlefish oil, krill oil,
`or mink oil, or an oil that was derived from oneof the above and had
`was concentrated with respect to eicosapentaenoic acid content.
`
`The concentration of oil having a high concentration of
`eicosapentaenoic acid in the present invention must be at least 10%
`of the volumeofliquid in the capsule. If this concentration 1s not
`achieved then the effect of the oil in the body will be lacking and as
`noted before the
`
`effects will not be sufficient. A nutritional supplement
`formed by melting vitamin F and soybean lecithin in oil
`with a high concentration of eicosapentaenoic acid, within
`the scope wherein it is possible to maintain the relative
`levels of the various substances, may be diluted with
`saffloweroil, rice oil, corn o11, soybean oil, or seaweed oil
`or other sea life oil. Mixed oil obtained in this way is
`inserted into a gelatin capsule under normal circumstances.
`As one example of the method of sealing the substance in
`a capsule, the mixed liquid will be sealed in a gelatin
`capsule in a certain amount, the gelatin formed by melting
`gelatin, glycerin, and water. After that, the entry hole will
`be heated and sealed to create the nutritional substance of
`the present invention.
`The form of the gelatin capsules may be spherical or in
`the form of a rugby ball.
`Because the nutritional supplement of the present
`invention obtained in this way contains vitamin E which
`prevents the aging of cells, lowers cholesterol numbers,
`prevents excess fat deposits, and has other positive side
`effects, as well as contains soybean lecithin, which acts to
`lower cholesterol and promote the absorption of
`eicosapentaenoic acid, and contains eicosapentaenoic acid,
`whichacts to prevent the hardening of blood vessels, the
`combined effects of the various ingredients act to reduce
`the cholesterol levels in the blood, prevent high blood
`pressure, reduce fat, and promote heart and brain function
`in brain and heart medical patients, and acts to treat and
`prevent adult circulatory diseases, and as such can besaid
`to functionas a nutritional supplement.
`
`Below, the embodiments of the present invention are explained.
`Embodiment 1.
`25 parts vitamin E and 25 parts soybean lecithin are dissolved into
`50 parts fish oil (containing 16% eicosapentaenoic acid), heated to
`around 60 degrees Celsius, and dried.
`Then 60 parts gelatin, 30 parts glycerin, and 10 parts water are
`uniformly mixed, arc made to take a film like form, are poured into a
`300 milligram capsule and put into a gelatin container. The
`aforementioned compound1s poured into this container, the entry
`point is heat scaled, and the nutritional supplement of the present
`invention is formed.
`
`[Embodiment 2
`30 parts vitamin F by weight and 30 parts soybean lecithin are
`dissolved into a 1:1 mixture of safflower oil and market concentrated
`
`eicosapentaenoic acid oil (produced by Nippon Yushi Co. Ltd., Sun
`Omega, and containing 25% eicosapentaenoic acid), the mixture
`being 40 parts. The liquid is heated to around 60 degrees Celsius and
`dried.
`Then 60 parts gelatin, 30 parts glycerin, and 10 parts water are
`uniformly mixed, are made to take a film like form, are poured into a
`300 milligram capsule and put into a gelatin container. The
`aforementioned compound is pouredinto this container, the entry
`point is heat sealed, and the nutritional supplement of the present
`invention is formed.
`
`-364-
`
`RIMFROST EXHIBIT 1024 page 0472
`RIMFROST EXHIBIRAR847 |PeRosh541
`000002
`
`

`

`(19) PBMSRT CJ P)
`
`(11)PFN
`BAIE8 — 231391
`(43) 43BRH én 8 4F(1996) 9 A108
`
`az) 9 Be ee EF Se BROAD
`
`(51) Int.CL.* aS=|AES FI Bewizen
`
`
`A61K_ 31/20
`AAM
`A61K 31/20
`AAM
`
`
`
`SAAR APR ARROKMS OL 4 4 A)
`
`(21) EAH
`
`fe— 36362
`
`(22) NB
`
`3A 7 AF (1995) 2 2A
`
`(71) HHA,
`
`
`
`392030380
`RARIMR
`FRA 4 TB 421
`(71) HERA 000173762
`SERETR
`fia RRRTA AT A 41
`592197599
`
`(71) HHL,
`
`AaeoR
`AURATHRARY 4 | E3832HH
`(2)RM —R
`PeaR) DAARSETRR 1 —28—10
`(2) Aw BA AIR
`SRAERT 3 — 5 —29
`RKRA ICS
`
`64 FRO) AiR
`
`
`(57) (3)
`
`
`
`
`[Bey] FERSwereTS. REAOA
`FACETS RES SZ
`(fie) RittsayReRa: LCS
`SIE BRL ST SREUE
`(3) WEIL SARUM. HA, SLABS
`OGL. SMM, hos Oe SSR, bE
`
`E72OE E ARPBREIOURF. FIRMIOWEPF.
`TRIBE OIEPSO FIBERPeeTA
`
`000001
`
`RIMFROST EXHIBIT 1024 page 0473
`RIMFROST EXHIB8247/pegosh451 4
`
`AKBM 1076
`
`

`

`
`
`
`
`CaraROFE ]
`(ack 1) Biyasda Re ake LOS
`APS CERRR SE T 4 ARIE.
`Cat 2] FRADE SRC
`&. RRIA 1 (CaCOIERCE
`
`CaS) RIED AREIC EL ABRROUIP .
`UAB. MARIROHHL. HbA S., BLUE
`SHRMCHA . HPRIA 2 (7CREARLSE
`Cae) RREIRAaE (2 E 4 FORRREO
`CHS. PRIA 1 (THROMAIACTaE.
`CHRIS]
`FURPRREOIRTP2*.ELS LE ABTARE
`
`FOUR. FIRTHF. BURrRRE OE
`
`PCH S. MORIA 1 (CHROREA.
`[SHA OPEAZREA]
`fo001]
`
`(messLovellsySh) ASTRALaE ICE3ERS
`UWS SRAIICED LL. BRICEEL <Ik, RAs
`BRtawmac lL CPate, Cee hot 4 eee
`WEAICATS LATHS!.
`fo002}
`
`
`CesT) PEOAO ORMEICCEY SS BEES
`SAORS a2 ELAb ETE
`Mk&woCwhS, PAR. AERO BMORPEE
`COUAER. AAVUSMARIRCOh aTAARICLO, BR
`
`
`ee OFSBYE LC RRLeH Ge SF Ld
`ASAE LCHHBS ATA, TORR DBD
`SEKUSE © LCR SAC Brees, SOReds
`LiMEL a Shree yr Om HBL se,
`
`BSCR. Saee. SRRSSORIEAe HS Ce
`BS < EOORIENT SRLS
`WW,
`[0003) R2VASHTVRIPOHOmee
`(226< RENTS PET. PI RYRA
`JPRS SAE AL TAC E BAST
`4, POOP, BompoAAeeehe ars
`CePWSHTHY. BAI.RECSSS!
`BEATSA. aCRaa URTA. UAE
`FPRERS AS Settee CRP 2 —
`
`AQ7 23-5) . 20>fPebPEseal CREPE 1 — 279
`
`
`
`8305) . WRIAAl CHRIS 7-35512
`=) SOT HMEMA SAWS, THEO, TERA
`
`F2-AQTABRHLRAGASHLY BLASBE
`Feaa Ose BOMRSROBFW 2 BS Betz
`LEWSItT SS APE OUGEIZOVC SARA
`mildest, £7e. BPE 1-2 7983085 Fa
`Ase ie OU YY DAF SI CEGATCAA
`DAV RFF SYOEOBIERAUIIT SF ECB
`SbLOTHA,
`fo004]
`[ORR L EGET SB) ARIS. BERS
`
`(2)
`
`HEPES —231391
`
`CIEETS. BIE)VREeR
`SAL EAS.
`foOo05]
`
`(eRe BRAS 4OTR] ARR IS, PRE
`
`ELORSAMGNWS FIPAS HL eeEB
`BCHT OL. CORREIAEC ICS iS EL
`Vid BACALL ICO S . ASRHA SERS SICB ore,
`[O006) F245. ARH. KAAoR
`eAEL CRASS CEE REL ST SER
`PRES HET S
`[O007 ) ARAORSVERUEES SAE
`ae. TaeCE. ePIC?
`WY IN4EROEE SEK Cl)
`Ald, BROWT. tA. SAARIROBIL, bos
`AAS. PE) HAVRECLAWS. #P
`BHENORPT. HORT. BexiHeoikPa) ae
`
`(GHASHA,
`
`
`
`[OO008 ) A3HHAIC AVS BaAaa RE
`WHER. OH DAP. FUEU RL UY
`HE DUDES. PALE BREA. ry SB
`(EASES BIRT SEACH S. CORDA LY
`Beet: Lis, BEL<aRa
`AyA GREER LO) OOS eM1 O%MLLE
`ALABPRS. CALIZOAMOBELITZL AVY,
`
`WIN PY a Pe Ve EOE OM Lee
`WH VF OCA VA EOEEAOURNabe
`fal, VEPRO. AP SU. 2 eT
`
`&Ofna EAT Ee Lee Lo
`BF SILA,
`[0009 ) ASSIAORRAOAcE
`
`OBSVUSBIGTS CECE S, POR
`SAL CISA, FURAN, LA, BEA Ze EO
`
`WIA AVE Oy PHOe aeFO
`
`
`
`EFS TALBCRA, Hee, JREOHSAMS LOC ERA
`LPTSHSIEMCES, CRE MBAULTTERA ICS
`BY, AAMAS iABSA Soe IZED
`aihicht 2 CEES RS, BicanoReitc £O Rpsctt
`BAK TSE LWHECHS, SRECSIAIS AVS
`Aid, ARORAMERSE AA BoA
`(HEREER > LC) OBLAIRS 1 O~1 OORE
`
`8%. WEL <SI50~1 O0CHE CHS.
`
`[O010) F3t8anay> UCik, AHRASSAY GEO
`
`Al) . CERT ABO GERAD)
`. FRRSA C282,
`
`ha—+F, AAR) | PMR CRC. HEARSE) eee
`
`
`
`feSRBC OS7 4 AARade OA SHA
`(0011) MAM, BOARS EURSAICH oT
`is. A CP: Wei. FUE AgeeOA FL
`Wee APTAS VER VP ALO A Hoke
`4A). AAA CP: ARALRPE RLF
`IVR LY XR FILER AAEE es) AR CS:
`
`
`
`000002
`
`RIMFROST EXHIBIT 1024 page 0474
`RIMFROST EXHIBR847Pem08b4515
`
`

`

`
`
`AFPULERVI RY AL PNP) AA P YS
`(fl: ER RY eAelO— A, ABR,
`bE ROS
`PORE A-A, BUR SDILERU EY) , FSU
`
`eA TRA AEM SIS
`(0012) FiaAle Bey 4 (claaishMeese
`Ly BR BES SITS CHRO ORARUAIS EO
`ZEHSVUZAP TU YERV TRL OAL PIP EEA
`TARA IZ CFTSESTUE LV. CHS ORLREA
`ib FREE PREVAFVENR-AVIYL—bh.L A
`PPIUNB, AP? IRS PILAR Ve EA
`
`IRAPESEACAGE LCRPE, AS VULOPe
`BQ-A. Abe, We ECS LC RE
`MAE TAIL LCKS!. Fre, APeee!
`(ZHANAeITS ERR
`PEENEEAAVWSA VU EU Y PULL YF Ua
`Sk i, AU ee EI CREL OBS FY
`CREEL Ray Te LAE TSO LBCRS,
`[0013] BORSA ORISA BET 4 clade
`peop e BRE YILE BL, PU EU SYEORATE
`
`Be 7b TARE LC BH Oy PH, BIA, Le
`VERMA TLUSL NAME TAR PREP.
`kKoFv Rk. BPUYIAA-—E SOL AREAP
`VA AP RUT AGE eI CT FALSE LOO
`BEM, CHEMISES EOFS, ee
`
`Al, HAA Ee MATRL EV,
`[OO14) S7ceestAlic KH 2 Cid, ARPECESTAN% fom
`L134 TSARVs LIBRO Cl: GEAFARek. AE
`Heiaek, POR YE PUL) . Ra Cal: Ae
`
`UVIEN— b 8 OEOFA) . p HESS
`Chl: APRS 7S ORE) . BER: CORA
`
`
`
`HEPES —231391
`
`
`SULERTES,
`
`
`[0016] &5tohAalle Ao Clk, aeeze vs LaPE
`OVA VW LIBRA Call: Pub — a. aR
`APN) . ReSe Ol: bP eR US
`2). SL{GA Cal : TAPER) Ze EPOBAa
`MESH, HBRSRS eas 4 chat
`AAAH. FRROKU, VPRUSL SUEUR RY
`EPL YP YAN SOAS & & Ode LCL
`LANL CAC CRAIT 4d. HERA ER UDF
`Aya, KEW EIR L ROB EDF LYECE
`PeTRuUTE,
`[0017] ath, batik Ad4a
`
`TOGBEIL. DHINSGRE. PIAS A ASE AS 1 ONR
`
`BUAMaraREze v3 La zekee PRIZE
`oCTbBETAC LE CRA,
`
`[O018) & < oRFRAOWIAEKCES , SAUE
`A (PIZITI O%LRLE) ORASTYBREA
`CNAOMECHSTOOK. RRPRIECHS LL
`Wa,
`
`[O01 9] AROFOEIRL)CH
`
`RIA Po RORas Boek ERS lz
`
`ORS A, fics—-A 4720, 100~2000
`
`
`me“MZECH O. -H 1 l~elicaiy CHeT
`4,
`
`
`
`
`(OO20)] LIF, AAAShePli2 EOPMICRA
`4,
`{0021]
`(eP]
`SAR 1. AEROSEER
`JaraRBS 1 3B Pvwe
`
`CPN? EPL) OO BooSot L
`C., fERORAMI ZINA TDHATOMEAYD APRILE
`1 O04@~ 2 OFFS (DIF, DHAHSBRY Wt) LD
`HAMPERS 6 > ATORhet L
`72. REC. TEROARAIIGES Hee cH Cel. Pee
`
`ARASH LWA 2 AS) BWR LC [apes 2 HAT
`L. DHAOAL HM Le, HEE 1 Zan
`Tv,
`[0022]
`(#1)
`
`AK DAME SHS
`[0015] RAE Ras 4 cea © ESS
`DHBHE AKBR(E? BU 72. FLA SLE PRUA
`YERKE RUA UY EROKSa RU Ae EA
`po HiGSA, HEP RUA 9 RR EP ORREAI
`“LEB ITEESTAREBKCLL ERP LO PY
`eS Be, Blevy ako, FY ARUY Le
`BFF ARU YY, CaF YEES CASPR
`L. FRARHOESAIE Lob kvl. vetearicLe
`Lhe RUYANA SO. BUELL
`Geb ye Ee INA CAH CFLES LEERSLA Eo
`#1. MAHERORBE
`
`
`
`ee|Pee|FE HB
`
` 1
`
`
`
`IaiERR
`
`TIMI Vea
`
`1
`
`2
`
`9
`
`0
`
`
`[0023] 28. JalSHAE tT SUGEOAE
`
`(a, HEA Aeee S PAHEPAA2 pl, FERAL
`
`000003
`
`RIMFROST EXHIBIT 1024 page 0475
`RIMFROST EXHIBIRAR847 |Pems08b4516
`
`
`
`

`

`(4)
`
`HEPES —231391
`
`ST U7HEPIA 3 Al EVOARRAS SUES
`30, ELCHMEATE 1 BIT HOR,
`FS
`Re PY eCRRRIZ BVT, RAK. LABS
`fA, SOS SHTICAD, BOBO, 1
`Bl,
`1 WCHore, EPR, COT, FERKOZEE
`WAG, See: FAM STZ:
`[0024] MERBl2.
`FAIPURRRIRPT OCSeaeat
`Ei
`
`aA1 ESO LC, TE, FURRar
`
`URE) 3 TEE 2 AI REOSe LA
`WAUSA be SPEERaE & CL
`fo, PRS, PRGA LH TRS > AOR 2 BIT, HH
`FRUCE Live. SEAS Olea,
`[0025]
`[#2]
`
`2.
`
`AMRBORMSE
`
`
`DHARS#H
`REE
`
`
`
`
`STARE 6rA|RRA|OARSat
`
`CRBRBALAR)
`
`
`AAA 6.243.3|6.943.0|3.442.9|3,143.3
`
`
`
`
`
`ABTARES 4,643.0|5.543.3|4142.9|2.6424
`
`
`
`
`
`
`
`
`
`
`EB 5.142.9|6.042.9|3.643.3|2.4428
`
`
`
`[0026 ] 48, 2-AWARBEYIO (SHE
`
`PE) AGRIC ISVS Cb RPCOA Ste,
`
`
`
`FOY KN YORS
`
`(72) 380 Bll B=k
`fis AERTR 1 — 2-12
`
`(72) She WR
`
`Hab A Sra7 S —27—-10
`
`000004
`
`RIMFROST EXHIBIT 1024 page 0476
`RIMFROST EXHTWR347 1PEM08O4517
`
`

`

`(19) Japan Patent Office (JP)
`
`(12) Patent Gazette (A)
`
`(51) Int. C16
`A61K 31/20
`
`Classification Symbol
`AAM
`
`Internal No.
`
`(11) Japanese Patent Application Publication Number
`Hei 8 — 231391
`(43) Publication Date September 10, 1996
`Technology Display Location
`FI
`A61K 31/20 AAM
`
`(21) Application Number
`(22) Application Date
`
`7 — 36362
`February 24, 1995
`
`
`
`Request for Review Unrequested Number of Claims 5 (Total 4 Pages)
`(71) Applicant
`392030380
`Kanagawa Kagaku Kenkyuujo Co. Ltd.
`Kanagawa-ken, Sumohara-shi, Nishi
`Onuma 4 Choume 4 Ban 1 Go
`000173762
`Sumo Central Chemical Research
`Location Foundation
`Kanagawa-ken, Sumohara-shi, Nishi
`Onuma 4 Choume 4 Ban | Go
`592197599
`Fuji Yakuhin Co. Ltd.
`Saitama-ken, Omiya-shi, Sakuragi Machi
`4 Choume 383 Banchi
`Ichira Yasawa
`Kanagawa_ken, Sumohara-shi, Kami-no-
`mori 1 — 28 —- 10
`Miyanaga Kazuo
`Gunma-ken, Macbashi-shi, Kanne Machi
`3-—5-29
`
`(71) Applicant
`
`(71) Applicant
`
`(72) Inventor
`
`(72) Inventor
`
`Continued onthe last page
`
`(54) [Title of the Invention] Medicine for Improvement of Dementia Symptoms
`
`(57) [Abstract]
`[Objective] To smoothly improve the symptoms of
`dementia and provide a medicine for said improvement
`without side effects.
`[Structure] A medicine for improvement of dementia
`symptomsthat has as a characteristic the inclusion of
`docosahexaenoic acid (DHA).
`[Effect] The medicine improves the following ailments
`caused by dementia: loss of will, delirium, worsening of
`human relationships, loitering, manic psychological
`episodes and/orthe reduction of powers of calculation,
`reduction ofjudgment, and reduction in the intellectual
`capacities and functioning of the higher functions.
`
`000001
`
`AKBM 1015
`RIMFROST EXHIBIT 1024 page 0477
`RIMFROST EXHIBE9347)pegos4518
`
`

`

`(2)
`
`[Scope of Claims]
`[Claim 1] A medicine for improvement of dementia symptoms
`being characterized by including as an active ingredient DHA.
`[Claim 2] A medicine for improvement of dementia symptoms
`of claim 1| that treats an adverse psychological state thatis
`dementia.
`[Claim 3] A medicine for improvement of dementia symptoms
`of claim 2 working to reduce will loss, delirium, worsening of
`humanrelationships, manic states, and/orloitering.
`[Claim 4] A medicine for improvement of dementia symptoms
`of claim 1 working to reduce the loss of higher functions and of
`judgment brought about by dementia.
`[Claim 5] A medicine for improvement of dementia symptoms
`of claim 1 working to reducethe loss of intellectual capacity,
`loss of facilities of calculation, loss of judgment, and/or loss of
`higher function due to dementia.
`
`[Detai