I lllll llllllll Ill lllll lllll lllll lllll lllll 111111111111111111111111111111111
`US010010567B2
`
`c12) United States Patent
`Bruheim et al.
`
`US 10,010,567 B2
`(IO) Patent No.:
`(45) Date of Patent:
`*Jul. 3, 2018
`
`(54) BIOEFFECTIVE KRILL OIL
`COMPOSITIONS
`
`(71) Applicant: AKER BIOMARINE ANTARCTIC
`AS, Stamsund (NO)
`
`(72)
`
`Inventors: Inge Bruheim, Valda (NO); Snorre
`Tilseth, Bergen (NO); Daniele
`Mancinelli, Orsta (NO)
`
`(73) Assignee: AKER BIOMARINE ANTARCTIC
`AS, Stamsund (NO)
`
`( *) Notice:
`
`Subject to any disclaimer, the term ofthis
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 15 days.
`
`This patent is subject to a terminal dis(cid:173)
`claimer.
`
`(21) Appl. No.: 15/589,605
`
`(22) Filed:
`
`May 8, 2017
`
`(65)
`
`Prior Publication Data
`
`US 2017/0246219 Al
`
`Aug. 31, 2017
`
`2250/708; A61K 31/202; A61K 31/685;
`A61K 35/612; A61K 9/0053; A61K
`9/4825; A61K 31/215; A61K 31/23;
`A61K 31/235; A61K 31/575; A61K 9/48;
`CllB 3/006; A23L 33/115
`See application file for complete search history.
`
`(56)
`
`References Cited
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`Related U.S. Application Data
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`FOREIGN PATENT DOCUMENTS
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`
`Int. Cl.
`A61K 9100
`A61K 9148
`A61K 311202
`A61K 311215
`A61K 311575
`A61K 311685
`A61K 351612
`A23L 331115
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`CPC .......... A61K 351612 (2013.01); A23L 331115
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`
`AU
`BR
`
`2002322233
`212003
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`8701265
`(Continued)
`
`OTHER PUBLICATIONS
`
`Institution Decision, Inter Partes Review Case No. IPR2017-00745,
`U.S. Pat. No. 9,078,905 B2, dated Aug. 16, 2017.
`Institution Decision, Inter Partes Review Case No. IPR2017-00747,
`U.S. Pat. No. 9,078,905 B2, dated Aug. 16, 2017.
`Institution Decision, Inter Partes Review Case No. IPR2017-00746,
`U.S. Pat. No. 9,028,877 B2, dated Aug. 16, 2017.
`Institution Decision, Inter Partes Review Case No. IPR2017-00748,
`U.S. Pat. No. 9,028,877 B2, dated Aug. 16, 2017.
`Action Closing Prosecution, 348 Patent, dated May 14, 2013.
`(Continued)
`
`Primary Examiner - Deborah K Ware
`(74) Attorney, Agent, or Firm - Casimir Jones S.C.
`
`ABSTRACT
`(57)
`This disclosure discloses new krill oil compositions charac(cid:173)
`terized by having high amounts of phospholipids, astaxan(cid:173)
`thin esters and/or omega-3 contents. The krill oils are
`obtained from krill meal using supercritical fluid extraction
`in a two stage process. Stage 1 removes the neutral lipid by
`extracting with neat supercritical C02 or C02 plus approxi(cid:173)
`mately 5% of a co-solvent. Stage 2 extracts the actual krill
`oils by using supercritical C02 in combination with approxi(cid:173)
`mately 20% ethanol. The krill oil materials obtained are
`compared with commercially available krill oil and found to
`be more bioeffective in a number of areas such as anti(cid:173)
`inflammation, anti-oxidant effects, improving insulin resis(cid:173)
`tances and improving blood lipid profile.
`
`20 Claims, 19 Drawing Sheets
`RIMFROST EXHIBIT 1001 Page 0001
`
`

`

`(56)
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`
`RIMFROST EXHIBIT 1001 Page 0003
`
`

`

`US 10,010,567 B2
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