`US007790869B2
`
`c12) United States Patent
`Ju et al.
`
`(IO) Patent No.:
`(45) Date of Patent:
`
`US 7,790,869 B2
`*Sep.7,2010
`
`(54) MASSIVE PARALLEL METHOD FOR
`DECODING DNA AND RNA
`
`(75)
`
`Inventors: Jingyue Ju, Englewood Cliffs, NJ (US);
`Zengmin Li, New York, NY (US); John
`Robert Edwards, New York, NY (US);
`Yasuhiro Itagaki, New York, NY (US)
`(73) Assignee: The Trustees of Columbia University
`in the City of New York, New York, NY
`(US)
`Subject to any disclaimer, the term ofthis
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 321 days.
`
`( *) Notice:
`
`This patent is subject to a terminal dis(cid:173)
`claimer.
`
`(21) Appl. No.: 11/810,509
`
`(22) Filed:
`
`Jun.5,2007
`
`(65)
`
`Prior Publication Data
`
`US 2008/0319179 Al
`
`Dec. 25, 2008
`
`Related U.S. Application Data
`
`(60)
`
`Continuation of application No. 10/702,203, filed on
`Nov. 4, 2003, now Pat. No. 7,345,159, which is a
`division of application No. 09/972,364, filed on Oct. 5,
`2001, now Pat. No. 6,664,079, and a continuation-in(cid:173)
`part of application No. 09/684,670, filed on Oct. 6,
`2000, now abandoned.
`
`(60)
`
`Provisional application No. 60/300,894, filed on Jun.
`26, 2001.
`
`(51)
`
`Int. Cl.
`C07H 21104
`C07H 21100
`C07H 19104
`Cl2Q 1/68
`
`(2006.01)
`(2006.01)
`(2006.01)
`(2006.01)
`
`(52) U.S. Cl. .................... 536/23.1; 536/25.3; 536/26.6;
`435/6
`(58) Field of Classification Search ..................... 435/6;
`536/23.1, 25.3, 26.6
`See application file for complete search history.
`
`(56)
`
`References Cited
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`
`(Continued)
`
`Primary Examiner-Jezia Riley
`(74) Attorney, Agent, or Firm-John P. White; Cooper &
`DunhamLLP
`
`(57)
`
`ABSTRACT
`
`This invention provides methods for attaching a nucleic acid
`to a solid surface and for sequencing nucleic acid by detecting
`the identity of each nucleotide analogue after the nucleotide
`analogue is incorporated into a growing strand of DNA in a
`polymerase reaction. The invention also provides nucleotide
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`able chemical group to cap the -OH group at the 3'-position
`of the deoxyribose.
`
`33 Claims, 28 Drawing Sheets
`
`NH,
`
`H
`
`,& " '~ ' ,
`
`I
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`0
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`OR
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`7
`
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`
`I
`
`3,.oi,A-,,., i....,~,2~nm
`
`Page 1
`
`Illumina Ex. 1013
`IPR Petition - USP 10,435,742
`
`
`
`US 7,790,869 B2
`Page 2
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`Appl. No. 11/894,808.
`PCT International Publication No. WO 08/069973 A2 Ju et al., pub(cid:173)
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