C U R R I C U L U M V I T A E
`KENNETH ALLEN JOHNSON
`Roger Williams Centennial Professor of Biochemistry
`Department of Molecular Biosciences
`2500 Speedway, A5000, MBB 3.122
`The University of Texas at Austin
`Austin, Texas 78712
`
`
`
`ACADEMIC TRAINING
`
`
`University of Iowa
`
`BS
`
`University of Wisconsin Ph.D.
`
`1971 Chemistry, with highest honors and with highest
`distinction.
`1975 Molecular Biology. Advisor: Dr. Gary Borisy
`Thesis Title: The Mechanism of Microtubule
`Assembly
`Postdoc 1979 With Edwin W. Taylor
`University of Chicago
`
` OCCUPATIONAL RECORD
`1998 - present Roger Williams Centennial Professor, Institute for Cellular and Molecular
`Biology, Dept. of Chemistry & Biochemistry, University of Texas at Austin,
`Austin, TX
`Paul Berg Professor of Biochemistry
`Department of Biochemistry and Molecular Biology,
`The Pennsylvania State University, University Park, PA
` Associate Professor, Department of Molecular and Cell Biology,
`The Pennsylvania State University, University Park, PA
`Assistant Professor, Department of Biochemistry and Biophysics,
`The Pennsylvania State University, University Park, PA
`Postdoctoral Fellow, Department of Biophysics and Theoretical Biology, The
`University of Chicago, Chicago, IL (with Dr. Edwin W. Taylor)
`
`
`HONORS AND AWARDS
`2018
`Fellow of the Biophysical Society
`2017
`Distinguished Alumnus, Davenport Central High School, Davenport, Iowa
`2012
`Vincent du Vigneaud Honorary Lectureship, University of Rochester
`2007
`Fellow of the American Association for the Advancement of Science
`2000
`Joseph Coleman Memorial Lecturer, Yale University, October 30, 2000
`1998
`Roger Williams Professorship, University of Texas at Austin
`1989
`Pfizer Award in Enzyme Chemistry, American Chemical Society
`1987
`Paul Berg Professorship, Pennsylvania State University
`
`1987
`Penn State Faculty Scholar Medal for Life and Medical Sciences
`1983-1988
`American Heart Association Established Investigatorship
`1979
`Muscular Dystrophy Association Postdoctoral Fellowship
`1976-1979
`National Institutes of Health Postdoctoral Fellowship
`1971
`Phi Beta Kappa
`
`1987-1998
`
`1984-1987
`
`1979-1984
`
`1975-1979
`
`
`
`Columbia Ex. 2016
`Illumina, Inc. v. The Trustees
`of Columbia University
`in the City of New York
`IPR2020-01177
`
`

`

`C U R R I C U L U M V I T A E
`KENNETH ALLEN JOHNSON
`Roger Williams Centennial Professor of Biochemistry
`Department of Molecular Biosciences
`2500 Speedway, A5000, MBB 3.122
`The University of Texas at Austin
`Austin, Texas 78712
`
`
`
`ACADEMIC TRAINING
`
`
`University of Iowa
`
`BS
`
`University of Wisconsin Ph.D.
`
`1971 Chemistry, with highest honors and with highest
`distinction.
`1975 Molecular Biology. Advisor: Dr. Gary Borisy
`Thesis Title: The Mechanism of Microtubule
`Assembly
`Postdoc 1979 With Edwin W. Taylor
`University of Chicago
`
` OCCUPATIONAL RECORD
`1998 - present Roger Williams Centennial Professor, Institute for Cellular and Molecular
`Biology, Dept. of Chemistry & Biochemistry, University of Texas at Austin,
`Austin, TX
`Paul Berg Professor of Biochemistry
`Department of Biochemistry and Molecular Biology,
`The Pennsylvania State University, University Park, PA
` Associate Professor, Department of Molecular and Cell Biology,
`The Pennsylvania State University, University Park, PA
`Assistant Professor, Department of Biochemistry and Biophysics,
`The Pennsylvania State University, University Park, PA
`Postdoctoral Fellow, Department of Biophysics and Theoretical Biology, The
`University of Chicago, Chicago, IL (with Dr. Edwin W. Taylor)
`
`
`HONORS AND AWARDS
`2018
`Fellow of the Biophysical Society
`2017
`Distinguished Alumnus, Davenport Central High School, Davenport, Iowa
`2012
`Vincent du Vigneaud Honorary Lectureship, University of Rochester
`2007
`Fellow of the American Association for the Advancement of Science
`2000
`Joseph Coleman Memorial Lecturer, Yale University, October 30, 2000
`1998
`Roger Williams Professorship, University of Texas at Austin
`1989
`Pfizer Award in Enzyme Chemistry, American Chemical Society
`1987
`Paul Berg Professorship, Pennsylvania State University
`
`1987
`Penn State Faculty Scholar Medal for Life and Medical Sciences
`1983-1988
`American Heart Association Established Investigatorship
`1979
`Muscular Dystrophy Association Postdoctoral Fellowship
`1976-1979
`National Institutes of Health Postdoctoral Fellowship
`1971
`Phi Beta Kappa
`
`1987-1998
`
`1984-1987
`
`1979-1984
`
`1975-1979
`
`
`
`

`

`1971
`1967-1971
`
`Chemistry Faculty Undergraduate Scholar Award
`Thomas Dooley Memorial Scholarship
`
`
`COMMITTEES
`National and International Committees
`2015
`Chair, Enzyme Mechanisms Conference
`2001-2006
`Member of Editorial Board of the Journal of Biological Chemistry
`1996-2004
`External reviewer for DFG Priority Program on Molecular Motors (Germany)
`1998
`Ad Hoc member of AIDS Study Section, National Institutes of Health
`1997
`Program Chair, Biological Division of American Chemical Society
`1996
`Chair, Gordon Conference on Biopolymers
`1989-1998
`Brookhaven STEM/NIH Advisory Committee
`1992
`Chair, Gordon Conference on Enzymes, Coenzymes & Metabolic Pathways
`1987-1988
`American Heart Association Grant Review Panel
`1986-1991
`Member of Cell Biology Study Section, National Institutes of Health
`1986
`Review Panel for the New Jersey Dept. of Higher Education
`1985-1988
`Monitoring Editor for the Journal of Cell Biology
`1983
` Organizing committee for the Cytoplasmic Matrix Conference
`1983
`Ad Hoc member of Cell Biology Study Section, NIH
`University Committees
`2016-2019
`Head, Biochemistry Graduate Studies Committee
`2015-2018
`Faculty Workload Committee
`2013-2017
`Chair, Graduate Student Travel Award Committee
`2007-2010
`Member, Advisory Board of Institute for Cellular & Molecular Biology
`2006-2008
`Department of Chemistry and Biochemistry Promotion and Tenure Committee
`2006-2008
`College Science Promotion and Tenure Committee
`2005
`College Committee for MGM Department research space assignment
`2004
`Chair, College Review of Organized Research Units
`2004
`Chair, Review of Waggoner Alcohol Addiction Center
`1999-2004
`Institute for Cell & Molecular Biology Advisory Committee
`1999-2003
`Coordinator for Biochemistry Division of Chemistry & Biochemistry Dept.
`2002
`Chair, Chemistry & Biochemistry Search Committee
`2001
`Chair, Chemistry & Biochemistry Search Committee
`1999
`Chair, Chemistry & Biochemistry Search Committee
`1996
`College of Science Promotion and Tenure Committee
`1994-1996
`Departmental Promotion and Tenure Committee
`1994-1998
`Endowed Faculty Search Committee
`
`
`
`
`
`Page 2 of 28
`
`

`

`Faculty Search Committee, Chairman
`Endowed Faculty Position Search Committee, Chairman
`Faculty Search Committee
`Graduate Student Search Committee, Chairman
`College of Science Promotion and Tenure Committee
`Departmental Nominations Committee, Chairman
`Departmental Headship Search Committee, Chairman
`College of Science Dean Search Committee
`Departmental Graduate Candidacy, Chairman
`Departmental Promotion and Tenure Committee, Chairman
`Biochemistry Program Graduate Candidacy, Chairman
`Graduate Student Admissions
`Faculty Search Committee
`Graduate Student Admissions
`
`1993-1996
`1992
`1991
`1990
`1989
`1988-1989
`1987-1989
`1987-1989
`1987
`1987
`1986
`1985-1986
`1985-1986
`1980-1983
`
`2011-2014
`2008-2012
`2007
`
`2005
`
`2004
`
`2003-2004
`1994-2001
`
`1999-2001
`1996-1997
`
`1987-1990
`
`
`
`
`
`
`
`
`CONSULTING ACTIVITIES
`1987-present President, KinTek Corporation, State College, PA
`2005-present Consultant and Member of Scientific Advisory Board of Pacific
`Biosciences
`Consultant, Novartis Vaccines & Diagnostics, Emeryville, CA
`Consultant, Roche Pharmaceuticals, Palo Alto, CA and Nutley, NJ
`Fish & Richardson – Expert witness for patent lawsuit on the use of
`reverse transcriptase in PCR.
`Drinkler Biddle & Reath – expert witness on lawsuit relating to the
`toxicity of AZT in treating AIDS.
`Fish & Richardson – expert witness on patent infringement lawsuit
`involving use of polymerases in PCR
`Consultant for Applied Biosystems Group, Applera Corp
`Consultant for Applied Biosystems Division, Perkin Elmer Corporation,
`Foster City, CA
`Consultant for Schering-Plough
`Expert witness for PCR patent law for Finnegan, Henderson, Farabow,
`Garrett & Dunner, Washington, DC
`Consultant for Monsanto Agricultural Co., St. Louis, MO
`
`
`
`
`
`
`
`Page 3 of 28
`
`

`

`RESEARCH INTERESTS
`
`Research in the Johnson Lab is focused on three different areas. The projects are linked by the
`common thread of using transient kinetic methods to examine enzyme reaction pathways and
`to relate our kinetic and functional analysis to enzyme structure.
`
`
`HIV Reverse Transcriptase mechanism, fidelity, inhibition and drug resistance. In
`previous work we have established the elementary steps leading to correct nucleotide
`incorporation by HIV reverse transcriptase and have quantified the changes in individual kinetic
`constants occurring during misincorporation. In addition, we have determined the mechanism of
`action of a class of nonnucleoside inhibitors and characterized changes leading to resistance
`against these agents. In current work, we are continuing to explore the mechanisms of multiple
`drug resistance and had provided an understanding of the role of enzyme conformational
`changes in enzyme specificity. A better understanding of these phenomena at the structural
`and mechanistic level can lead to the development of better combination therapies in the
`treatment of AIDS.
`
`Mitochondrial DNA Polymerase mechanism, fidelity and inhibition by nucleoside
`
`analogs. Several studies point to the likely role of the mitochondrial DNA polymerase in the
`toxicity of nucleoside analogs used in the treatment of viral infections such as hepatitis and
`AIDS. We have established that the toxicity of nucleoside analogs is correlated with their
`incorporation into mitochondrial DNA by the mitochondrial polymerase, spanning six orders of
`magnitude. We are currently examining the role of mutations in the human mitochondrial DNA
`polymerase that are linked to heritable human diseases. Our studies include detailed kinetic
`analysis of mutants and unique physiological studies in humanized yeast where the yeast
`mitochondrial DNA polymerase is replaced with the human orthologue.
`
`
`Mechanism of RNA-dependent RNA replication by the Hepatitis C viral polymerase.
`Hepatitis C infect 3% of the worlds population and chronic infection leads to liver cirrhosis and
`cancer. We are currently working to understand the mechanisms of initiation and elongation of
`RNA polymerization and the mechanisms of action of drugs currently under development. Here
`we take advantage of our recent success in finding conditions for the formation of a stable,
`highly active elongation complex. We showed how the kinetics of incorporation versus
`nucleotide-dependent excision explain why Sofosbuvir (a UMP analog) is an effective drug,
`while a similar cytosine analog fails.
`
`
`Mechanistic basis for CRISPR-Cas9 specificity. CRISPR-Cas9 offers the promise of
`effective gene therapy, but it is limited by off-target effects. In our current work, we are
`investigating the kinetic and thermodynamic basis of enzyme specificity and evaluating several
`“high fidelity” variants. This work provides a guide for enzymes with improved specificity.
`
`
`SARS-CoV-2 RNA-dependent RNA polymerase. In our current work we have
`reconstituted the viral polymerase and have provide kinetic analysis to explain why Remdesevir
`is an effective inhibitor used to treat COVID-19. This work lays the foundation for development
`of new direct acting antiviral drugs.
`
`
`
`
`
`
`
`
`Page 4 of 28
`
`

`

`CURRENT RESEARCH GRANT SUPPORT
`
`
`NIH/NIAID: 1R01 AI110577-01A1 (Johnson)
`
`12/01/14 to 11/30/2020 (no
`Dynamics of Hepatis C viral RNA-dependent RNA replication
`
`cost extension)
` Hepatitis C virus (HCV) infects approximately 180 million people worldwide, including 4-5 million
`in the USA, and chronic infection leads to liver cirrhosis and cancer. The previous standard of care,
`involving 48 weeks of PEGylated interferon and ribavirin is only partially effective and causes
`significant side effects, but has been enhanced by antiviral nucleoside analogs. New nucleoside and
`nonnucleoside inhibitors have been developed that act directly against the HCV RNA-dependent
`RNA polymerase. In this proposal, we will use newly established methods to form an active
`polymerase-RNA elongation complex to establish the mechanism of action of these new inhibitors
`and quantify the effects of mutations leading to resistance. This research will provide a better
`foundation for the design and evaluation of more effective drugs to treat HCV infections.
`
`NIH/NIGMS: 1R01 GM114223-01 (Johnson)
`
`04/01/15 to 01/31/2021 (no
`Correlating defects in mitochondrial DNA replication to
`
`cost extension)
`physiology
`Mutations in the human mtDNA polymerase (Pol γ) have been correlated with various mitochondrial
`disorders, including mtDNA depletion syndrome, Alpers Syndrome, and progressive external
`opthalmoplegia (PEO), and many of the nucleoside analogs used to treat HIV infections have toxic
`side effects due to inhibition of mitochondrial DNA (mtDNA) replication. This research will provide a
`better understanding of the role of the mtDNA polymerase in diseases related to mitochondrial
`function, and will provide new information to define the molecular basis for nucleotide discrimination
`by the human mtDNA polymerase, the physiological basis for the toxicity of nucleoside analogs used
`to treat HIV infections, and the role of mtDNA polymerase and helicase mutations in heritable
`disorders.
`
`The Welch Foundation: F-1604 (Johnson)
`
`06/01/2008 to 05/31/2020
`Kinetics of Hepatitis C Viral RNA-dependent RNA Polymerase
`
`This grant provides seed money to develop new methods to examine the kinetic basis for nucleotide
`selectivity by the Hepatitis C viral RNA polymerase and the effectiveness of nucleoside analogs
`used to treat HCV infections.
`
`
`
`
`
`
`
`
`Page 5 of 28
`
`
`
`
`
`
`
`
`
`

`

`TEACHING
`
`
`COURSE NO.
`
`TITLE
`
`Biochem 41
`Biochem 401
`Biochem 425
`Biochem 451
`Biochem 503
`Biochem 525
`Biochem 496
`Biochem 600
`Chemistry 394
`Biochem 394
`
`YEARS
`TAUGHT
`1
`4
`3
`2
`2
`15
`17
`17
`16
`7
`
`TOTAL
`STUDENTS
`92
`901
`227
`49
`40
`420
`38
`55
`475
`150
`
`Introductory Biochemistry
`General Biochemistry
`Introductory Physical Biochemistry
`Senior Seminar
`Biochemical Problems
`Proteins and Enzymes
`Independent Study
`Graduate Research
`Chemistry of Enzymes
`Structure and Dynamics of Proteins
`and Nucleic Acids
`
`Annual Kinetics Workshop:
`For the past 15 years I have taught a 4-day intensive workshop on modern enzyme
`kinetics. With more than 450 graduates, the workshop is attended by 40-50 individuals
`each year with professors, graduate students and industrial research scientists in
`approximately equal proportions, from the US and Europe, and as far away as Russian
`and Malaysia. The workshop is funded by registration fees paid by the participants.
`
`Software development:
`Through KinTek Corporation, I have hired programmers and worked closely with them
`to write a computer program for fitting kinetic data based upon computer simulation.
`Based upon numerical integration of rate equations, the program allows data to be fit
`directly to a model, bypassing the simplifying assumptions required for mathematical
`modeling. Licenses for a professional version of the software are offered for sale to
`support the programming effort. A free student version is also available at
`http://www.kintekexplorer.com. The professional version is available free of charge to
`faculty and students of the University of Texas.
`
`
`
`
`
`
`
`
`
`
`
`
`Page 6 of 28
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`

`

`7.
`
`SEMINARS PRESENTED
`
`1. Princeton University, Department of Biology. “The Mechanism of ATP Hydrolysis by
`Actomyosin.” May 23, 1979
`2. University of Guelph, Department of Chemistry. Two seminars: “Thermodynamics of
`Microtubule Assembly” and “The Pathway of GTP Hydrolysis During Microtubule
`Assembly.” Dec. 1, 1980
`3. Friday Harbor Washington, International Meeting on The Mechanism and Control of Ciliary
`Movement. “Transient-State Kinetic Analysis of the Dynein ATPase.” Sept. 10, 1981
`4. Worcester Foundation for Experimental Biology, Shrewsbury, MA. “The Structure of Dynein
`ATPase and the Mechanism of Force Production for Microtubule Sliding.” Feb. 17, 1982
`5. Yale University, New Haven, CT, Department of Biology. “The Structure of the Dynein
`ATPase and the Mechanism of Force Production for Microtubule Sliding.” Feb. 19, l982
`6. University of Pennsylvania, Philadelphia, PA, Department of Biochemistry and Biophysics.
`“Kinetic and Structural Analysis of the Dynein ATPase.” May 25, 1982
`International Conference on Cilia and Flagella, Siena, Italy. “Structural and Mass Analysis
`of Dynein by Scanning Transmission Electron Microscopy.” July 12, l982
`8. Max Planck Institute for Medical Research, Heidelberg, West Germany, Department of
`Biophysics. “Structural and Kinetic Analysis of Dynein-microtubule Complex.” July 16,
`1982
`9. Woods Hole Marine Biological Laboratory, Woods Hole, MA. “Structural and Kinetic
`Analysis of Dynein.” Aug. 10, 1982
`10. Carnegie-Mellon University, Pittsburgh, PA. Department of Biological Sciences. “Kinetic
`and Structural Analysis of the Dynein-Microtubule Complex.” Sept. 29, 1982
`11. California Institute of Technology, Pasadena, CA, Division of Biology. “Structure and
`Kinetics of the Dynein ATPase.” Feb. 18, 1983
`12. Brandeis University, Waltham, MA. Department of Biochemistry. “Biochemistry of Dynein-
`microtubule Interaction.” Mar. 2, 1983
`13. National Institutes of Health, NIHLB, Bethesda, MD. “Structure and Kinetics of the Dynein
`ATPase.” Sept 19, 1983
`14. NICHD Testis Workshop Symposium, National Institutes of Health, Bethesda, MD.
`“Scanning Transmission Electron Microscopy of Dynein Arms.” Invited symposium
`lecture. Oct. 16, 1983
`15. Cytoplasmic Matrix Conference, Fogarty International Center, Bethesda, MD. “Mechanisms
`of Force Production for Intracellular Movements.” Invited symposium lecture. Oct. 19,
`1983
`16. Temple University School of Medicine. “Structure and Function of Dynein, the Motor for
`Ciliary Movement.” Mar. 21, 1984
`17. Woods Hole Marine Biological Laboratory, Woods Hole, MA. “Structure of Dynein and the
`Mechanism of the Microtubule-Dynein ATPase.” June 28, 1984
`18. American Chemical Society Meeting, Philadelphia, PA. Organizer and speaker at
`symposium on “Protein-Macromolecule Interactions.” “Structure and ATPase Mechanism
`of Dynein and its Role in Microtubule-Dependent Movements.” Aug. 27, 1984
`
`
`
`
`
`
`
`Page 7 of 28
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`

`

`19. U. S.-Japan Cooperative Science Program on Fundamental Problems of Movement of Cilia,
`Eukaryotic Flagella and Related Systems, Hakone, Japan. “Dynein Structure and the
`Pathway of the Microtubule-Dynein ATPase.” Sept. 7, 1984
`20. Hershey Medical Center, Pennsylvania State University, Hershey, PA, Dept. of Biological
`Chemistry. “Structure and Mechanism of the Dynein ATPase and its Interaction with
`Microtubules.” Sept. 24, 1984
`21. Northwestern University Medical School, Dept. of Cell Biology and Anatomy, Chicago, IL.
`“Structure of Dynein and the Mechanism of Interaction with Microtubules.” Oct. 22, 1984
`22. International Chemical Congress of Pacific Basin Societies, Honolulu, HI. “Microtubule-
`Dynein Interactions and the Pathway of ATP Hydrolysis.” Dec. 19, 1984
`23. University of Pittsburgh, School of Medicine, Dept. of Biochemistry, Pittsburgh, PA.
`“Structure of Dynein and the Pathway of the Microtubule-Dynein ATPase.” Mar. 21, 1985
`24. DESY, Max-Planck-Gesellschaft, Colloquim in “Molekulare Strukturbiologie,” Hamburg,
`West Germany. “Structure and Function of the Dynein ATPase.” May 13, 1985
`25. Yamada Conference on “Energy Transduction in ATPases.” Kobe, Japan. “Dynein
`Structure and the Pathway of the Microtubule-Dynein ATPase.” May 27, 1985
`26. Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Kimball Union
`Academy. “Dynein Structure and the Pathway of the Microtubule-Dynein ATPase.” July
`12, 1985
`27. Princeton University, Dept. of Chemistry, Princeton, NJ. “Structure and Mechanism of the
`Dynein ATPase.” Aug 13, 1985
`28. American Society for Cell Biology Meeting, Subgroup meeting on Dynein, organized and
`presented paper, Atlanta, GA. Nov 17, 1985
`29. Brandeis University, Dept. of Biochemistry, Waltham, MA. “Structure and Mechanism of the
`Dynein ATPase.” Dec 4, 1985
`30. SUNY-Buffalo, Cell Motility Minisymposium, Buffalo, NY. “Structure of Dynein and the
`Mechanism of Force Production for Microtubule-Dependent Movements.” Feb 17, 1986
`31. Johns Hopkins University School of Medicine, Dept. of Anatomy & Cell Biology, Baltimore,
`MD. “Structure of Dynein and the Mechanism of Force Production for Microtubule-
`Dependent Movements.” Mar 4, 1986
`32. University of Utah, Department of Biochemistry, Salt Lake City, Utah. “Structure and
`Mechanism of the Microtubule-Dynein ATPase.” Mar 11, 1986
`33. Harvard University, Dept. of Biology, Cambridge, MA. “Structure and mechanism of the
`Microtubule-Dynein ATPase.” Mar 19, 1986
`34. British Society for Cell Biology Meeting, Norwich, England. “Dynein and Related Proteins in
`Cell Motility.” BSCB Symposium. Apr 8, 1986
`35. State University of New York, Cellular and Developmental Biology, Stony Brook, NY.
`“Structure and Mechanism of the Dynein ATPase.” May 23, 1986
`36. Gordon Research Conference, Tilton, NH. “Mechanism of the Microtubule-Activated,
`Dynein ATPase.” July 24, 1986
`37. University of Colorado, Dept. of Molecular, Cellular and Developmental Biology, Boulder,
`CO. Two seminars: “Structure and Mechanism of the Microtubule-Dynein ATPase” and
`“Elementary steps in the DNA Polymerase I Reaction Pathway.” Sept. 25, 1986
`
`
`
`
`
`
`
`Page 8 of 28
`
`

`

`38. Monsanto Agricultural Co., St. Louis, MO. “Transient Kinetic Analysis of Enzyme Reaction
`Mechanisms: Implication for Inhibitor Design.” Oct. 17, 1986
`39. Scripps Clinic and Research Foundation, Dept. of Cellular and Developmental Immunology,
`La Jolla, CA. “Structure and Pathway of the Microtubule-Dynein ATPase.” March 4, 1987
`40. American Society of Biological Chemists, Annual Meeting, Philadelphia, PA. Invited
`Symposium Talk: “Dynein.” June 8, 1987
`41. Electron Microscope Society of America Annual Meeting, Baltimore, MD. Invited
`Symposium Talk: “Scanning Transmission EM of Dynein ATPases.” August 7, 1987
`42. Texas A & M, Dept. of Biochemistry and Biophysics, College Station, TX. “Elementary
`Steps in the DNA Polymerase I Reaction Pathway.” November 11, 1987
`43. University of Maryland, Department of Chemistry & Biochemistry, College Park, MD.
`“EPSP Synthase: Enzymatic Pathway and Mechanism of Action of the Herbicide,
`Glyphosate.” March 1, 1988.
`44. Gordon Research Conference on Cellular and Molecular Biology of the Plant and Fungal
`Cytoskeleton, Proctor Academy, Andover, NH. “Structure and Pathway of the
`Microtubule-Activated Dynein ATPase.” August 11, 1988
`45. International Conference on Force Production and Microtubule-Coupled Cell Movement,
`Stowe, VT. “Dynein Structure and Function.” August 23, 1988
`46. Bioscience Advisory Committee Meeting on Macromolecular Structure and Drug Design,
`Johnson and Johnson Conference Center, New Brunswick, NJ. “Alteration of Protein
`Structure and Effect on Biological Activity.” October 12, 1988
`47. Brandeis University, Department of Biochemistry, Waltham, MA. “EPSP Synthase:
`Enzymatic Pathway and Mechanism of Action of the Herbicide, Glyphosate.” October 26,
`1988
`48. Penn State, Hershey Medical Center, Cell Biology Program, Hershey, PA. “Structure and
`Mechanism of the Dynein ATPase.” March 15, 1989
`49. Institute for Cancer Research, Philadelphia, PA. “Mechanism of DNA Polymerase I.”
`March 16, 1989
`50. American Chemical Society Meeting, Dallas, TX, Pfizer Award Address. “Solving Enzyme
`Mechanisms by Transient Kinetics.” April 11, 1989
`51. Symposium in Molecular Biology, DNA-Protein Interactions, Pennsylvania State University,
`University Park, PA. “Pathway and Fidelity of DNA Polymerization.” July 27, 1989
`52. Department of Biochemistry, St. Louis University, St. Louis, MO. “Pathway and Fidelity of
`DNA Polymerase.” Sept. 6, 1989
`53. Pfizer Chemical Co., Groton, CN. “EPSP Synthase Mechanism: Implications for Inhibitor
`Design.” Oct. 10, 1989
`54. Fogarty International Center Symposium on “Kinetic Approaches to the Study of
`Physiological Functions, National Institutes of Health, Bethesda, MD. “DNA Polymerase:
`Kinetic Mechanism and Fidelity.” Oct. 18, 1989
`55. Department of Chemistry, University of Maryland, Baltimore, MD “Mechanism and Fidelity
`of DNA Replication.” Feb. 13, 1990
`56. Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH.
`“Substrate Channeling: Facts and Misinterpretations.” July 5, 1990
`
`
`
`
`
`
`
`Page 9 of 28
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`

`

`57. Gordon Research Conference on Mutagenesis. Plymouth State College, Plymouth, NH.
`“Mechanistic Basis of DNA Polymerase Fidelity." July 11, 1990
`58. Department of Biochemistry, Duke University Medical School, Durham, NC. “Mechanistic
`Basis for DNA Polymerase Fidelity.” Oct 5, 1990
`59. Department of Microbiology, New Jersey School of Medicine, Newark, NJ. “Mechanistic
`Basis for DNA Polymerase Fidelity.” Oct 23, 1990
`60. Department of Chemistry, Wayne State University, Detroit, MI. “Mechanistic Basis for DNA
`Polymerase Fidelity.” Oct 29, 1990
`61. Department of Biochemistry, Johns Hopkins School of Public Health, Baltimore, MD.
`“Mechanistic Basis for DNA Polymerase Fidelity.” Dec. 10, 1990
`62. Department of Pharmacology, Yale University Medical School, New Haven, CT.
`“Mechanistic Basis for DNA Polymerase Fidelity.” Dec. 18, 1990
`63. Department of Chemistry and Biochemistry, University of Delaware, Newark, DE.
`“Mechanistic Basis for DNA Polymerase Fidelity.” Sept. 6, 1991
`64. Department of Chemistry, Cornell University, Ithaca, NY. “Kinetic and Thermodynamic
`Basis for DNA Polymerase Fidelity.” Sept. 25, 1991
`65. Royal Society, London, England “Kinetic and Thermodynamic Basis for DNA Polymerase
`Fidelity.” Oct. 25, 1991
`66. Department of Biochemistry, University of Minnesota, Minneapolis, MN. “Kinetic and
`Thermodynamic Basis for DNA Polymerase Fidelity.” Jan 8, 1992
`67. National Institutes of Health, Bethesda, MD. “Mechanism and Inhibition of HIV Reverse
`Transcriptase.” April 7, 1992
`68. Boeringer-Ingelheim, Danbury, CT. “Mechanism and Inhibition of HIV Reverse
`Transcriptase.” Oct. 6, 1992
`69. Center for Advanced Research in Biotechnology, University of Maryland, Rockville, MD.
`“Mechanism and Inhibition of HIV Reverse Transcriptase.” Oct. 26, 1992
`70. Japanese Biophysical Society Meeting, Osaka, Japan. “Conformational Coupling in DNA
`Replication.” Nov. 6, 1992
`71. Brystol-Myers-Squibb Pharmaceutical, Princeton, NJ. “Mechanism and Inhibition of HIV
`Reverse Transcriptase.” Dec. 10, 1992
`72. Parke-Davis Pharmaceutical, Ann Arbor, MI. “Mechanism and Inhibition of HIV Reverse
`Transcriptase.” Jan 18, 1993
`73. University of Michigan, Dept. of Biochemistry, Ann Arbor, MI. “Mechanism and Inhibition of
`HIV Reverse Transcriptase.” Jan 19, 1993 (Annual graduate student invited speaker).
`74. Washington University, Dept. of Biochemistry and Molecular Biophysics, St. Louis, MO.
`“Mechanism and Inhibition of HIV Reverse Transcriptase.” Feb. 3, 1993
`75. National Institutes of Health, Bethesda, MD. “HIV Reverse Transcriptase Mechanism and
`Inhibition.” Feb. 19, 1993
`76. Department of Chemistry, University of Rochester, Rochester, NY. Albert Noyes/Mobay
`Memorial Lectures I: “Detection of Enzyme Intermediates: Lessons from EPSP Synthase
`and Tryptophan Synthase.” Feb. 22, 1993
`77. Department of Chemistry, University of Rochester, Rochester, NY. Albert Noyes/Mobay
`Memorial Lectures II: “DNA Polymerase Fidelity and Error Correction.” Feb 23, 1993
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`78. Department of Chemistry, University of Rochester, Rochester, NY. Albert Noyes/Mobay
`Memorial Lectures III: “HIV Reverse Transcriptase Mechanism and Inhibition.” Feb. 24,
`1993
`79. International Conference on Perspectives in AIDS Research, Heidelberg, Germany. “HIV
`Reverse Transcriptase Mechanism and Inhibition.” April 20, 1993
`80. Department of Biochemistry, Ohio State University, Columbus, OH. “HIV Reverse
`Transcriptase Mechanism and Inhibition.” May 18, 1993
`81. Department of Biochemistry, Temple University, Philadelphia, PA. “HIV Reverse
`Transcriptase Mechanism and Inhibition.” May 20, 1993
`82. Steenbock Symposium on Protein-DNA Interactions, Department of Biochemistry,
`University of Wisconsin, Madison, WI. “HIV Reverse Transcriptase Mechanism and
`Inhibition.” May 24, 1993
`83. Department of Human Biological Chemistry and Genetics, University of Texas Medical
`Branch, Galveston, TX. “HIV Reverse Transcriptase Mechanism and Inhibition.” Dec. 16,
`1993
`84. Biophysical Society Meeting, Invited Symposium Talk. “Mechanisms of DNA
`Polymerization.” Feb. 16, 1994
`85. Department of Chemistry and Biochemistry, University of Maryland-Baltimore County,
`Baltimore, MA. “Mechanisms of DNA Polymerization.” March 21, 1994
`86. Gordon Conference on Biopolymers, Newport, RI. “Energy Transduction Mechanisms.”
`June 27, 1994
`87. Rutgers University, Center for Advanced Biotechnology and Medicine. “HIV Reverse
`Transcriptase Mechanism and Inhibition.” July 16, 1994
`88. Biophysical Discussions Meeting, Airlie, VA. “Kinesin ATPase Mechanism.” October 22,
`1994
`89. Michigan State University, Department of Biochemistry, East Lansing, MI. “HIV Reverse
`Transcriptase Fidelity and the Mechanism of Inhibition by Non-nucleoside Inhibitors”.
`December 5, 1994
`90. Vanderbilt University, Nashville, KY. “Mechanism of HIV Reverse Transcriptase Inhibition
`by Non-nucleoside Inhibitors”. December 9, 1994
`91. Albert Einstein College of Medicine, Bronx, NY. “Mechanism of HIV Reverse Transcriptase
`and Mode of Inhibition by Non-nucleoside Inhibitors.” December 20, 1994
`92. Biophysical Society Meeting, San Francisco, CA. “The New Enzymology.” February 12,
`1994
`93. National Institutes of Health, National Heart, Lung and Blood Institute, Bethesda, MD.
`“Pathway of Processive ATP Hydrolysis by Kinesin.” March 27, 1995
`94. University of North Carolina, Department of Biochemistry, Chapel Hill, NC. “Pathway of
`Processive ATP Hydrolysis by Kinesin”. April 18, 1995
`95. Fidelity Conference. Sponsored by National Institutes of Environmental Health Science,
`Wrightsville Beach, NC. “Mechanism of HIV Reverse Transcriptase and Mode of Inhibition
`by Non-nucleoside Inhibitors.” September 12, 1995
`96. Brandeis University, Department of Biochemistry, Waltham, MA. “Pathway of processive
`ATP hydrolysis by kinesin.” December 6, 1995
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`97. University of Arizona, Department of Biochemistry, Tucson, AZ. “Mechanism and Inhibition
`of HIV Reverse Transcriptase.” September 6, 1996
`98. EMBO Workshop, Xanten, Germany, “Effects of RNA Secondary Structure on HIV Reverse
`Transcriptase.” October 3, 1996
`99. EMBO Workshop, Xanten, Germany, “Alternating Site Mechanism of Kinesin ATPase.”
`October 4, 1996
`100. Ohio State University, Columbus, OH. “Mechanism and Inhibition of HIV Reverse
`Trascriptase.” February 17, 1997
`101. Biophysical Society Meeting, New Orleans, LA. “HIV Reverse Transcriptase: Mechanism
`of Reading through hairpins.” March 2, 1997
`102. Salk Institute, La Jolla, CA . “HIV Reverse Transcriptase Mechanism, Inhibition and
`Resistance.” April 11, 1997
`103. Department of Biochemistry & Molecular Biology, Hershey Medical Center, Hershey, PA.
`“HIV Reverse Transcriptase mechanism, inhibition and resistance.” May 5, 1997.
`104. Department of Biochemistry & Biophysics, University of North Carolina, Chapel Hill, NC.
`“HIV Reverse Transcriptase Mechanism, Inhibition and Resistance.” June 23, 1997
`105. Gordon Conference on Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH.
`“Alternating Site Mechanism of the Kinesin ATPase.” July 14, 1997
`106. International Conference of Energy Transduction and Regulation, Bochum, Germany.
`“Alternating Site Mechanism of the Kinesin ATPase.” October 6, 1997
`107. Organon Teknika, Boxtel, Netherlands. “HIV Reverse Transcriptase Mechanism, Inhibition
`and Resistance.” October 8, 1997