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`
`Agilent Exhibit 1264
`Page 1 of 143
`
`
`
`

`

`PTC-0200 DNA Engine &
`PTC-0225 DNA Engine Tetrad
`Operations Manual
`
`
`Version 4.0Version 4.0
`Version 4.0
`
`Version 4.0Version 4.0
`
`MJ Research, Inc.
`
`Waltham, Massachusetts
`
`
`Agilent Exhibit 1264
`Page 2 of 143
`
`

`

`Copyright ©1999, MJ Research, Inc. All rights reserved. Reproduction in any form, either print or
`electronic, is prohibited without written permission of MJ Research, Inc.
`
`DNA Engine, DNA Engine Tetrad, Alpha, Hot Bonnet, Power Bonnet, Microseal, Chill-Out, Self-
`Seal and Twin Tower are trademarks of MJ Research, Inc.
`
`05434-05
`
`Thermal cyclers can be used for a number of purposes, including the polymerase chain reaction
`(PCR). PCR is covered by patents owned by Hoffmann-La Roche, Inc., and F. Hoffmann-La Roche,
`Ltd., who have granted exclusive and nonexclusive licenses for some types of applications. Roche
`and its licensees provide end-user licenses within their respective fields. These licenses have dif­
`ferent terms depending on the particular application of PCR, and different rules may apply in
`different countries. Anyone who intends to use MJ Research equipment to do PCR is encouraged
`to contact Hoffmann-La Roche for more information, at one of the addresses below:
`
`In the United States:
`
`In other nations:
`
`Kathe Kiehn
`Licensing Manager
`Roche Molecular Systems, Inc.
`1145 Atlantic Avenue
`Alameda, CA 94501 USA
`(510) 814-2970
`Fax: (510) 814-2977
`
`Dr. Agnieszka Junosza-Jankowski
`PCR Licensing Manager
`F. Hoffmann-La Roche Ltd.
`Building 222/350
`CH-4002 Basel, Switzerland
`41-61-687-3031
`Fax: 41-61-687-2113
`
`Agilent Exhibit 1264
`Page 3 of 143
`
`

`

`Contents
`
`
`Documentation Conventions ............................................................................................... iv
`
`
`Part I: The DNA Engine
`
`1. Introduction ................................................................................................................. 1-1
`
`2. Layout and Specifications .......................................................................................... 2-1
`
`3. Installation.................................................................................................................... 3-1
`
`4. Operation...................................................................................................................... 4-1
`
`5. Running Protocols ....................................................................................................... 5-1
`
`6. Creating Programs ...................................................................................................... 6-1
`
`7. Editing Programs ........................................................................................................ 7-1
`
`8. Using the Utilities........................................................................................................ 8-1
`
`9. Networking ..................................................................................................................9-1
`
`10. Maintenance ................................................................................................................. 10-1
`
`11. Troubleshooting ........................................................................................................... 11-1
`
`
`Part II: The DNA Engine Tetrad
`
`12. Information Specific to the DNA Engine Tetrad..................................................... 12-1
`
`
`Part III: Accessories
`
`13. The Remote Alpha Dock System .............................................................................. 13-1
`
`
`Appendix A: Safety Warnings and Guidelines ............................................................. A-1
`
`Appendix B: How a Peltier Heat Pump Works ............................................................. B-1
`
`Appendix C: How a Switching Power Supply Works ................................................. C-1
`
`Appendix D: Shipping Instructions for US Residents.................................................. D-1
`
`Appendix E: Warranties .................................................................................................... E-1
`
`Appendix F: Factory-Installed Protocols ........................................................................ F-1
`
`
`Index ..................................................................................................................................... In-1
`
`
`Agilent Exhibit 1264
`Page 4 of 143
`
`

`

`Documentation Conventions
`
`Typographic Conventions
`The names of keyboard keys are set in sans serif type and encased in
`
`double angle brackets:
`
`
`
`ExampleExample
`Example
`
`ExampleExample
`
`«Proceed»
`
`
`Items in programming menus are italicized:
`
`
`
`ExampleExample
`Example
`
`ExampleExample
`
`Select Edit from the Main Menu.
`
`
`Graphic Conventions
`The programming screens displayed in the LCD window are represented
`by a box containing four lines of text:
`
`
`ExampleExample
`Example
`
`ExampleExample
`
`Run: 2-STEP
`
` 1= 92.0 for 0:05
`
`Cycle: 1
`
`Calc: 65.0
`
`Terminology
`
`A programming option is termed “selected” when the cursor is positioned
`in front of it. Use the «Select» keys (see fig. 2-2) to move the cursor. In
`some screens selected items are also displayed in all-capital letters.
`
`iviviviviv
`
`Agilent Exhibit 1264
`Page 5 of 143
`
`

`

`Part I
`
`
`The DNA Engine
`
`
`Agilent Exhibit 1264
`Page 6 of 143
`
`

`

`Agilent Exhibit 1264
`
`Page 7 of 143
`
`Agilent Exhibit 1264
`Page 7 of 143
`
`

`

`1
`
`Introduction
`
`
`Meet the DNA Engine, 1-2
`
`Using This Manual, 1-2
`
`Important Safety Information, 1-3
`
`
`1-11-11-11-11-1
`
`Agilent Exhibit 1264
`Page 8 of 143
`
`

`

`DNA Engine & DNA Engine Tetrad Operations Manual
`
`Meet the DNA Engine
`Thank you for purchasing an MJ RESEARCH PTC-200 DNA Engine. De­
`signed by a team of molecular biologists and engineers, the DNA Engine
`will meet your needs for a versatile, easy-to-use, reliable, and compact
`programmable thermal cycler:
`• Interchangeable sample blocks—the Alpha unit family— that accom­
`modate many types of tubes, microplates, and slides
`
`• Hot Bonnet heated lid (or its remote-controlled version, the Power Bon­
`net) for oil-free cycling
`
`• Intuitive software with easy-to-read interface for quick and painless
`
`programming, editing, file management, password protection, and
`much more
`
`• Choice of calculated sample temperature control for highest speed
`and accuracy, or block or probe control for compatibility with proto­
`cols designed for a variety of instrument types
`
`• Space-saving design for easy setup and transportation
`
`• Instant Incubate feature for continuous-temperature incubations
`• Networking for up to 15 machines, for convenient remote operation
`and documentation of runs
`
`• Customizable factory-installed protocols
`When multiple users or high-throughput operations must be accommo­
`dated, the PTC-225 DNA Engine Tetrad is available. Essentially consist­
`ing of four DNA Engines placed in one chassis, the DNA Engine Tetrad
`has the same features and ease of use of the stand-alone DNA Engine.
`
`Using This Manual
`This manual contains all the information you need to operate your DNA
`Engine or DNA Engine Tetrad safely and productively:
`
`• Chapter 2 acquaints you with the physical characteristics of the DNA
`Engine.
`• Chapters 3–5 present the basics of installing and operating the DNA
`Engine.
`
`• Chapters 6 and 7 describe programming the DNA Engine.
`• Chapter 8 outlines the utilities available for the DNA Engine.
`
`• Chapter 9 describes how to network and remotely operate the DNA
`Engine and DNA Engine Tetrad.
`
`1-21-21-21-21-2
`
`Agilent Exhibit 1264
`Page 9 of 143
`
`

`

`Introduction
`
`• Chapter 10 explains the proper maintenance of the DNA Engine.
`
`• Chapter 11 offers troubleshooting information for the DNA Engine.
`
`• Chapter 12 describes the operation of the DNA Engine Tetrad.
`• Chapter 13 describes the operation of the RAD–0200 Remote Alpha
`Dock accessory.
`
`Important Safety Information
`Safe operation of the DNA Engine or DNA Engine Tetrad begins with a
`complete understanding of how the machine works. Please read this en­
`tire manual before attempting to operate the DNA Engine or DNA En­
`gine Tetrad. Do not allow anyone who has not read this manual to oper­
`ate the machine.
`
`The DNA Engine and DNA Engine Tetrad can generate enough heat to
`inflict serious burns and can deliver strong electrical shocks if not used
`according to the instructions in this manual. Please read the safety warn­
`ings and guidelines in appendix A, and exercise all precautions outlined
`in them.
`
`1-31-31-31-31-3
`
`Agilent Exhibit 1264
`Page 10 of 143
`
`

`

`Agilent Exhibit 1264
`
`Page 11 of 143
`
`Agilent Exhibit 1264
`Page 11 of 143
`
`

`

`2
`
`Layout and Specifications
`
`
`Front View, 2-2
`
`Control Panel, 2-2
`
`Back View, 2-3
`
`Bottom View, 2-3
`
`Alpha Units, 2-4
`
`Single-Block Models, 2-4
`
`Dual-Block Models, 2-4
`
`Slide Block, 2-4
`
`Power Bonnet Accessory, 2-4
`
`Specifications, 2-5
`
`Gradient Specifications, 2-6
`
`
`2-12-12-12-12-1
`
`Agilent Exhibit 1264
`Page 12 of 143
`
`

`

`DNA Engine & DNA Engine Tetrad Operations Manual
`
`Front View
`
`(Figure 2-1)
`
`Thumbwheel
`
`Alpha unit, lid closed
`
`Control panel
`
`CAUTION: Sample block(s) may be very hot !
`ATTENTION: Les blocs peuvent erte tres chauds !
`A Block Status
`B
`Heating
`Cooling
`
`DNA Engine
`
`Instant Incubate
`
`Power
`
`Block Display
`
`Select
`
`MJ RESEARCH
`
`PTC-200
`Peltier Thermal Cycler
`
`Pause
`
`Stop
`
`Proceed
`
`Cancel
`
`Air exhaust vents
`(also on other side)
`
`Air intake vents
`
`Control Panel
`(Figure 2-2)
`
`Block status lights
`
`Instant incubation key
`
`Power light
`
`2-22-22-22-22-2
`
`Block key
`
`DNA Engine
`
`CAUTION:Sample blocks may be very hot !
`^ `
`ATTENTION: Les blocs peuvent etre tres chauds !
`
`A
`
`Block Status
`Heating
`Cooling
`
`B
`
`Instant Incubate
`
`Block
`
`Select
`
`Power
`
`MJ RESEARCH
`
`PTC-200
`P eltier Thermal Cycler
`
`1
`4
`7
`.
`
`P ause
`
`2
`5
`8
`0
`
`3
`6
`9
`–
`
`Stop
`
`Proceed
`
`Cancel
`
`LCD window
`
`Left and right
`selection keys
`
`Proceed key
`
`Cancel key
`
`Agilent Exhibit 1264
`Page 13 of 143
`
`

`

`Layout and Specifications
`
`Air exhaust vents
`(also on other side)
`
`Air intake vents
`
`RS-232 port
`
`IEEE-488 port
`
`Parallel printer port
`
`Back View
`
`(Figure 2-3)
`
`Alpha unit
`
`Alpha unit handle
`
`Power cord jack
`
`Bottom View
`(Figure 2-4)
`
`Air intake vents
`
`Back
`
`Front
`
`2-32-32-32-32-3
`
`Agilent Exhibit 1264
`Page 14 of 143
`
`

`

`DNA Engine & DNA Engine Tetrad Operations Manual
`
`Alpha Units
`
`
`Single-Block Models
`60V Alpha: Holds 60 x 0.5ml tubes
`
`
`
` 96V Alpha: Holds 96 x 0.2ml tubes or one 96-well
`microplate
`192 Alpha: Holds one 192-well microplate
`
` 384 Alpha: Holds one 384-well microplate or
`
`
`one 96-well microplate
`
`Dual-Block Models
`
`30/30 Dual Alpha: Holds 2 x 30 x 0.5ml tubes
`
`30/48 Dual Alpha: Holds 1 x 30 x 0.5ml tubes
`and 1 x 48 x 0.2ml tubes
`
`48/48 Dual Alpha: Holds 2 x 48 x 0.2ml tubes or
`half plates
`
`Slide Block
`Twin Tower: Holds 2 x 16 standard slides
`
`Power Bonnet Accessory
`Permits remote control of Alpha unit lid opening; avail­
`able for Alpha models 96V and 384.
`
`2-42-42-42-42-4
`
`Agilent Exhibit 1264
`Page 15 of 143
`
`

`

`Specifications
`Thermal range:
`
`Accuracy:
`
`Thermal homogeneity:
`
`Ramping speed:
`
`Sample capacity:
`
`Line voltage:
`
`Frequency:
`
`Power:
`
`Fuses:
`
`Displays:
`
`Ports:
`
`Memory:
`
`Weight:
`
`Layout and Specifications
`
`–5˚ to 105˚C, but no more than 30˚C below ambient
`temperature
`±0.3˚C of programmed target @ 90˚C, NIST-traceable
`±0.4˚C well-to-well within 30 seconds of arrival at
`90˚C (for most Alpha units; see specifications for in­
`dividual Alpha units)
`Up to 3˚C/sec for all single- and dual-block Alpha
`units; 1.2˚C/sec for the Twin Tower
`
`Varies with installed Alpha unit
`100–240VAC rms (no adjustment needed among volt­
`ages within these ranges)
`
`50–60Hz single phase
`850W maximum
`
`Two 6.3A, 250V, 5 x 20mm
`
`One 20 x 4 LCD alphanumeric display
`One 25-pin 8-bit parallel interface printer port
`
`One 9-pin RS-232 serial port for printer or remote use
`
`One IEEE-488 bidirectional general purpose interface
`bus
`
`200 typical programs in up to 12 individual folders
`
`7.6–9.3kg, varies with installed Alpha unit (DNA
`Engine Tetrad: 29kg, including Alpha units and
`power supply)
`
`Size:
`
`24 x 35 x 17cm (DNA Engine Tetrad: 37 x 55 x 25cm)
`
`2-52-52-52-52-5
`
`Agilent Exhibit 1264
`Page 16 of 143
`
`

`

`Gradient Specifications (96V Alpha module only)
`
`+ 0.4°C of programmed target at end columns, 30
`Accuracy:
`seconds after the timer starts for the gradient step,
`NIST–traceable
`+ 0.4°C , well–to–well within column, within 30 sec­
`onds of reaching target temperature
`+ 0.4°C of actual well temperature
`30°C
`
`Column uniformity:
`
`Calculator accuracy:
`
`Lowest programmable
`temperature
`
`Highest programmable
`temperature
`
`Temperature differential
`range
`
`105°C
`
`1–24°C
`
`Agilent Exhibit 1264
`Page 17 of 143
`
`

`

`3
`
`Installation
`
`
`Packing Checklist, 3-2
`Setting Up the DNA Engine, 3-2
`Environmental Requirements, 3-2
`Power Supply Requirements, 3-3
`Air Supply Requirements, 3-3
` Ensuring an Adequate Air Supply, 3-3
`
`Ensuring That Air Is Cool Enough, 3-4
`Requirements for Robotics Installations, 3-5
`
`
`3-13-13-13-13-1
`
`Agilent Exhibit 1264
`Page 18 of 143
`
`

`

`DNA Engine & DNA Engine Tetrad Operations Manual
`
`Packing Checklist
`After unpacking the DNA Engine, check to see that you have received
`the following:
`
`• One DNA Engine base
`• One Alpha unit (more if additional units were ordered)
`
`• Two spare fuses
`
`• One power cord
`• One probe, if purchased
`
`• PTC-200 DNA Engine & PTC-225 DNA Engine Tetrad Operations Manual
`(this document)
`• Product registration card (US customers only)
`
`• Extended warranty application (US customers only)
`
`• DNA Engine Driver remote software request form
`If any of these components are missing or damaged, contact MJ RESEARCH
`or the authorized distributor from whom you purchased the DNA En­
`gine to obtain a replacement. Please save the original packing materials
`in case you need to return the DNA Engine for service. See appendix E
`for shipping instructions.
`
`Setting Up the DNA Engine
`The DNA Engine requires only minimal assembly: plugging in the power
`cord and inserting an Alpha unit. Insert the power cord plug into its jack
`at the back of the machine (see fig. 2-3 for location of jack), then plug the
`
`
` cord into an electrical outlet. With the machine turned off, insert an Alpha
`unit (see “Installing an Alpha Unit,” chapter 4).
`
`
`Caution:Caution:
`Caution: Do not insert or remove an Alpha unit with the DNA
`
`Caution:Caution:
`Engine turned on; electrical arcing can result. Read the
`safety warning in appendix A regarding electrical safety
`when inserting or removing an Alpha unit.
`
`Environmental Requirements
`Ensure that the area where the DNA Engine is installed meets the follow­
`ing conditions, for reasons of safety and performance:
`• Nonexplosive environment
`• Normal air pressure (altitude below 4000m)
`
`3-23-23-23-23-2
`
`Agilent Exhibit 1264
`Page 19 of 143
`
`

`

`Installation
`
`• Ambient temperature 5˚–31˚C
`• Relative humidity up to 90%
`
`• Unobstructed access to air that is 31˚C or cooler (see below)
`
`• Protection from excessive heat and accidental spills (Do not place
`the DNA Engine near such heat sources as radiators, and protect it
`from danger of having water or other fluids splashed on it, which
`can cause shorting in its electrical circuits.)
`
`Power Supply Requirements
`The DNA Engine requires 100–240VAC, 50–60Hz, and a grounded out­
`let. The DNA Engine can use current in the specified range without
`adjustment, so there is no voltage-setting switch.
`
`Power cords for outlets other than the US 120V outlet may be purchased
`from computer stores, since they are also used for most desktop computers
`and printers and meet international standard IEC-320. The power cord must
`be rated to carry at least 10A at 125V or 250V, depending on the voltage
`available in your nation. The quality of the power cord can be further en­
`sured by making certain it is inscribed with the trademark of UL, CSA, TUV,
`VDE, or another national testing agency.
`
`NNNNNote:ote:ote:ote:ote: Do not cut the supplied 120V power cord and attach a different con­
`nector. Use a one-piece molded connector of the type specified above.
`
`Air Supply Requirements
`The DNA Engine requires a constant supply of air that is 31˚C or cooler in
`order to remove heat from the Alpha unit’s heat sink. Air is taken in from
`vents at the front, back, and bottom of the machine and exhausted from
`vents on both sides (see figs. 2-1, 2-3, and 2-4). If the air supply is inadequate
`or too hot, the machine can overheat, causing performance problems, soft­
`ware error messages (particularly “HS Overheating” and “Slow Block Cy­
`cling”), and even automatic shutdowns. Special attention should be paid to
`airflow and air temperature in robotics installations of DNA Engines.
`
`Ensuring an Adequate Air Supply
`• Do not block the air intake vents.
`
`Position the DNA Engine at least 10cm from vertical surfaces and
`other thermal cyclers (greater distances may be required; see be­
`low). Do not put loose papers under the machine; they can be sucked
`into the air intake vents on the bottom of the machine.
`
`3-33-33-33-33-3
`
`Agilent Exhibit 1264
`Page 20 of 143
`
`

`

`DNA Engine & DNA Engine Tetrad Operations Manual
`
`• Do not allow dust or debris to collect in the air intake vents.
`The bottom air vents are particularly liable to collect dust and debris,
`sometimes completely clogging up. Check for dust and debris every
`few months, and clean the intake vents as needed. Remove light col­
`
`
` lections of dust with a soft-bristle brush or damp cloth. Severe col­
`lections of dust and debris should be vacuumed out. Turn the ma­
`chine off prior to cleaning or vacuuming air vents.
`Ensuring That Air Is Cool Enough
`• Do not position two or more DNA Engines (or other thermal cyclers)
`so that the hot exhaust air of one blows directly into the air intake
`vents of another.
`• Make sure the DNA Engine receives air that is 31˚C or cooler by mea­
`suring the temperature of air entering the machine through its air
`intake vents.
`
`Place the DNA Engine where you plan to use it, and turn it on. Try to
`reproduce what will be typical operating conditions for the machine
`in that location, particularly any heat-producing factors (e.g., nearby
`equipment running, window blinds open, lights on). Run a typical
`protocol (e.g., 2-Step) for 30 minutes to warm up the DNA Engine,
`then measure the air temperature at the back air intake vents. If more
`than one machine is involved, measure the air temperature for each.
`
`If the air intake temperature of any machine is warmer than 31˚C,
`use table 3-1 to troubleshoot the problem. Some experimentation
`may be required to determine the best solution when more than one
`cause is involved. After taking steps to solve the problem, verify that
`
`the temperature of the air entering the air intake vents has been low­
`ered, using the procedure outlined above.
`
`Table 3-1 Troubleshooting Air Supply Problems
`
`Cause
`
`Possible Remedies
`
`Air circulation is poor.
`
`Provide more space around machine or adjust room ventilation.
`
`Ambient air temperature Adjust air conditioning to lower ambient air temperature.
`is high.
`Machine is in warm part Move machine away from, or protect machine from, such heat
`of room.
`sources as radiators, heaters, other equipment, or bright sunlight.
`Machines are crowded.
`Arrange machines so that warm exhaust air does not enter intake
`vents.
`
`3-43-43-43-43-4
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`Page 21 of 143
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`

`

`Installation
`
`Requirements for Robotics Installations
`Robotics installations require special attention to airflow and air tempera­
`ture. Typically in these installations, DNA Engines and other thermal cy­
`clers are crowded into a small area, along with other heat-generating equip­
`ment. Overheating can quickly occur when many of these machines are
`operating at once, unless preventive measures are taken.
`
`Follow the procedures described above to ensure adequate airflow and
`an air intake temperature of 31˚C or cooler. Air intake temperature must
`be verified by measurement.
`
`Do not use oil to thermally couple sample vessels to the blocks of ma­
`chines in a robotics installation. Oil makes plates difficult to remove.
`
`3-53-53-53-53-5
`
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`Page 22 of 143
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`

`

`Agilent Exhibit 1264
`
`Page 23 of 143
`
`Agilent Exhibit 1264
`Page 23 of 143
`
`

`

`4 Operation
`
`
`Turning the DNA Engine On, 4-2
`
`Understanding the Main Menu, 4-2
`
`Using the Control Panel, 4-3
`
`Operation Keys, 4-3
`
`Status Indicator Lights, 4-3
`
`Using the Data Ports, 4-3
`
`Operating Alpha Units, 4-4
`
`Installing an Alpha Unit, 4-4
`
`Removing an Alpha Unit, 4-5
`
`Opening an Alpha Unit, 4-5
`
`Closing an Alpha Unit, 4-6
`
`Selecting the Correct Sample Vessel, 4-6
`
`0.5-ml Tubes, 4-6
`
`0.2-ml Tubes, 4-6
`
`Microplates, 4-6
`
`Sealing Sample Vessels, 4-7
`
`Sealing with Oil or Wax, 4-7
`
`Sealing with the Hot Bonnet and Caps or Film Sheets, 4-7
`
`Adjusting the Hot Bonnet’s Lid Pressure, 4-8
`
`Loading Sample Vessels into the Block, 4-9
`
`Using Oil to Thermally Couple Sample Vessels to the Block, 4-9
`
`Using the Optional Probe, 4-10
`
`Customizing the Probe Vessel, 4-10
`
`Adding the Oil, 4-11
`
`Loading and Connecting the Probe, 4-11
`
`Detecting a Faulty Probe, 4-12
`
`Appendix 4-A: Tube, Microplate, and Sealing System Selection Chart, 4-13
`
`Appendix 4-B: Safety Warning Regarding Use of 35S Nucleotides, 4-14
`
`Appendix 4-C: Using Silicone Oil in the Probe Tube, 4-16
`
`
`4-14-14-14-14-1
`
`Agilent Exhibit 1264
`Page 24 of 143
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`

`

`DNA Engine & DNA Engine Tetrad Operations Manual
`
`Turning the DNA Engine On
`Move the power switch to “1” (the “On” position). In most The fan will
`turn on, and the power light on the keyboard will glow red. In most
`cases a self-test of the heat pumps will begin running (see note below).
`Its progress is tracked on a screen in the LCD window:
`
`PTC-200:
`
` Self testing
`
`.
`
`This screen disappears in 1 minute. If a problem is detected, the display
`shows an error message.
`Note:Note:Note:Note:Note: If the Alpha unit’s block or heat sink is not at ambient tempera­
`ture (typically because the Alpha unit was recently in use), the machine
`will skip the self-test.
`
`If the self-test does not detect any problems, the Main Menu is displayed:
`
`PTC-200:
`
`_RUN
`
`
`
` Enter
`
` List
`
`
`
` Edit
`
` Files
`
`
`
` Setup
`
`The DNA Engine is now ready to execute programs.
`
`Understanding the Main Menu
`The Main Menu is the common access point to all programming and
`machine configuration screens:
`
`• Run: Executes a program.
`
`• Enter: Allows new programs to be entered.
`
`• List: Accesses utilities that display or print a program’s steps.
`
`
`• Edit: Allows modification of stored programs.
`
`
`• Files: Accesses file management utilities.
`
`• Setup: Accesses machine and networking configuration screens.
`
`
`4-24-24-24-24-2
`
`Agilent Exhibit 1264
`Page 25 of 143
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`

`

`Using the Control Panel
`The control panel (see fig. 2-2) includes operation keys, status indicator
`lights, an LCD window for displaying programming and machine status
`text, and a numeric keypad for entering values into programs.
`
`Operation
`
`Operation Keys
`• Select keys (left and right arrows): Move the cursor one space or op­
`tion to the left or right in the LCD window; display time and cycle
`information during a protocol run.
`
` • Proceed: Accepts a selected menu or screen option; during a protocol
`
`run, advances the program to its next step.
`• Cancel: Terminates a running protocol; during program creation or
`editing, cancels the last entry.
`
`• Stop: Terminates a running protocol.
`• Pause: Pauses a protocol during execution; accesses Japanese Katakana
`syllabary.
`
`• Instant Incubate: Initiates a program that sets up the DNA Engine as
`a simpl