UNITED STATES PATENT AND TRADEMARK OFFICE
`
`BEFORE THE PATENT TRIAL AND APPEAL BOARD
`
`AGILENT TECHNOLOGIES, INC.
`Petitioner,
`v.
`BIO-RAD LABORATORIES, INC.,
`Patent Owner
`
`U.S. Patent No.: 8,236,504
`Filing Date: June 30, 2010
`Issue Date: August 7, 2012
`Title: Systems and Methods For Fluorescence Detection With a Movable Detection
`Module
`
`IPR Trial No.: TBD
`
`DECLARATION OF DR. JAMES P. LANDERS, PH.D. IN SUPPORT OF
`PETITION FOR INTER PARTES REVIEW OF U.S. PATENT NO.
`8,236,504 UNDER 35 U.S.C. §§311-319 AND 37 C.F.R. §42.100 et seq.
`
`Agilent Exhibit 1208
`Page 1 of 327
`
`

`

` 
`
`
`
`TABLE OF CONTENTS
`
`Page
`
`INTRODUCTION ......................................................................................... 1 
`I. 
`SUMMARY OF MY OPINIONS ................................................................. 3 
`II. 
`III.  QUALIFICATIONS AND BACKGROUND ............................................... 6 
`IV.  TECHNOLOGY BACKGROUND ............................................................. 10 
`V.  UNDERSTANDINGS OF LAW ................................................................. 21 
`VI.  THE ’504 PATENT ..................................................................................... 24 
`VII.  PROSECUTION HISTORY ....................................................................... 29 
`VIII.  PERSON OF ORDINARY SKILL IN THE ART....................................... 33 
`IX.  CLAIM CONSTRUCTION ........................................................................ 35 
`A. 
`“Heating element” / “Heater” .............................................................. 36 
`B. 
`“Wells” ................................................................................................ 39 
`PRIORITY DATE ....................................................................................... 40 
`A. 
`The terms “heating element” (claim 1) and “heater”
`(claim 13) are not adequately described in their parent
`applications, and thus the claims are not entitled to the
`benefit of an earlier priority date ......................................................... 41 
`The terms “a plurality of openings” (claim 1) or “a
`plurality of transparent portions” (claim 13) are not
`adequately described in their parent applications, and
`thus the claims are not afforded the benefit of an earlier
`priority date ......................................................................................... 44 
`XI.  STATE OF THE ART ................................................................................. 45 
`A. 
`Thermal Cycling PCR Machines......................................................... 46 
`B. 
`Use of Fluorescent Labeling and Detection to Perform
`
`X. 
`
`B. 
`
` 
`
`‐ii-
`
`Agilent Exhibit 1208
`Page 2 of 327
`
`

`

`C. 
`
`F. 
`
` 
`
`XII. 
`
` 
`
`Real-Time PCR ................................................................................... 47 
`Overview of International Patent Publication WO
`2004/104547 (“Kordunsky”) ............................................................... 50 
`D.  Overview of Publication No. CN1379236A (“Li”) ............................ 51 
`E. 
`Overview of U.S. Patent No. 5,528,050 to Miller, et al.
`(“Miller”) ............................................................................................. 54 
`Overview of U.S. Patent No. 6,730,883 to Brown, et al.
`(“Brown”) ............................................................................................ 56 
`G.  Overview of U.S. Patent No. 5,125,748 to Bjornson, et
`al. (“Bjornson”) ................................................................................... 60 
`H.  Overview of U.S. Patent No. 5,784,152 to Heffelfinger
`(“Heffelfinger”) ................................................................................... 61 
`INVALIDITY OF ALL CLAIMS OF THE ’504 PATENT ........................ 63 
`A.  Ground I: Claims 1-20, 22 of the ’504 Patent are
`anticipated by Kordunsky .................................................................... 64 
`1. 
`Claim 1 ...................................................................................... 65 
`a) 
`[Element 1pre] - “A fluorescence detection
`apparatus for analyzing samples located in a
`plurality of wells in a thermal cycler, the
`apparatus comprising” .................................................... 65 
`[Element 1a] - “a support structure
`attachable to the thermal cycler; and” ............................ 65 
`[Element 1b] - “a shuttle movably mounted
`on the support structure” ................................................. 65 
`[Element 1c] - “a detection module attached
`to the shuttle, the detection module
`including:” ...................................................................... 65 
`[Element 1d] - “a housing having an
`opening oriented toward the plurality of
`wells” .............................................................................. 66 
`[Element 1e] - “an excitation light generator
`disposed within the housing ........................................... 66 
`‐iii-
`
`b) 
`
`c) 
`
`d) 
`
`e) 
`
`f) 
`
`Agilent Exhibit 1208
`Page 3 of 327
`
`

`

`g) 
`
`h)
`
`i)
`
`j)
`
`k)
`
`[Element 1f] - “an emission light detector
`disposed within the housing,” ......................................... 66
`[Element 1g] - “wherein, when the support
`structure is attached to the thermal cycler,” ................... 67
`[Element 1h] - “a heating element is
`disposed between the detection module and
`the sample wells and” ..................................................... 67
`[Element 1i] - “the shuttle is movable to
`position the detection module in optical
`communication with different wells of the
`plurality of wells” ........................................................... 67
`[Element 1j] - “through a plurality of
`openings extending through the heating
`element.” ......................................................................... 67
`Dependent Claims 2-12 ............................................................. 68
`[Claim 2] The fluorescence detection
`a)
`apparatus of claim 1 wherein an excitation
`optical path from the excitation light
`generator to the opening has a fixed length
`and a detection optical path from the
`opening to the emission light detector has a
`fixed length. .................................................................... 68
`[Claim 3] The fluorescence detection
`apparatus of claim 1 further comprising: a
`calibration element disposed such that the
`detection module is movable so as to be
`positioned in optical communication with
`the calibration element, wherein the
`calibration element provides a known
`fluorescence response. .................................................... 68
`[Claim 4] The fluorescence detection
`apparatus of claim 3 wherein the calibration
`element is located between two or more of
`the plurality of wells. ...................................................... 69
`[Claim 5] The fluorescence detection
`apparatus of claim 3 wherein the calibration
`element is located in an area peripheral to
`the plurality of wells. ...................................................... 69
`‐iv-
`
`2.
`
` 
`
`b)
`
`c)
`
`d)
`
`Agilent Exhibit 1208
`Page 4 of 327
`
`

`

` 
`
` 
`
`3. 
`
`e) 
`
`f) 
`
`g) 
`
`h) 
`
`i) 
`
`[Claim 6] The fluorescence detection
`apparatus of claim 1 wherein positioning of
`the detection module with respect to the
`wells is controlled by an external computer. .................. 69 
`[Claim 7] The fluorescence detection
`apparatus of claim 1 wherein operation of
`the excitation light generator and the
`emission light detector is controlled by an
`external computer. .......................................................... 69 
`[Claim 8] The fluorescence detection
`apparatus of claim 1 wherein the detection
`module is detachably attached to the shuttle. ................. 70 
`[Claim 9] The fluorescence detection
`apparatus of claim 1 wherein the excitation
`light generator comprises a light-emitting
`diode. .............................................................................. 70 
`[Claim 10] The fluorescence detection
`apparatus of claim 1 further comprising at
`least two stepper motors mounted on the
`support structure, the stepper motors being
`operative to move the shuttle in at least two
`dimensions. ..................................................................... 70 
`[Claim 11] The fluorescence detection
`apparatus of claim 1 wherein the detection
`module includes at least two emission light
`detectors. ......................................................................... 70 
`[Claim 12] The fluorescence detection
`apparatus of claim 1 wherein the detection
`module includes at least two excitation light
`generators. ....................................................................... 71 
`Claim 13 .................................................................................... 71 
`a) 
`[Element 13pre] “A thermal cycler
`apparatus comprising:” ................................................... 71 
`[Element 13a] “a thermal cycler having an
`exterior housing and a plurality of sample
`wells for holding reaction vessels” ................................. 71 
`[Element 13b] “a heater to prevent
`‐v-
`
`j) 
`
`k) 
`
`b) 
`
`c) 
`
`Agilent Exhibit 1208
`Page 5 of 327
`
`

`

`d)
`
`e)
`
`f)
`
`g)
`
`h)
`
`i)
`
`j)
`
`k)
`
`condensation from forming on a surface of
`the reaction vessels when the reaction
`vessels are in the sample wells,” .................................... 71
`[Element 13c] “the heater having a plurality
`of transparent portions to permit optical
`communication with each of the plurality
`sample wells;” ................................................................. 72
`[Element 13d] - “a support structure
`disposed inside the exterior housing on an
`opposite side of the heater from the sample
`wells” .............................................................................. 72
`[Element 13e] - “a shuttle movably mounted
`on the support structure; and” ......................................... 72
`[Element 13f] - “a detection module
`attached to the shuttle, the detection module
`including: a module housing having an
`opening that is oriented toward the plurality
`of sample wells when the thermal cycler is
`in an operating state;” ..................................................... 73
`[Element 13g] - “an excitation light
`generator disposed entirely within the
`module housing; and” ..................................................... 73
`[Element 13h] - “an emission light detector
`disposed entirely within the module
`housing;” ......................................................................... 73
`[Element 13i] - “when the thermal cycler is
`in the operating state, the shuttle is movable
`to position the detection module in optical
`communication with different sample wells
`of the plurality of sample wells” .................................... 73
`[Element 13j] - “through the transparent
`portions of the heater.” ................................................... 74
`Dependent Claims 14-20 and 22 ............................................... 74
`[Claim 14] The thermal cycler apparatus of
`a)
`claim 13 wherein the excitation light
`generator comprises a light-emitting diode. ................... 74
`[Claim 15] The thermal cycler apparatus of
`‐vi-
`
`4.
`
` 
`
`b)
`
`Agilent Exhibit 1208
`Page 6 of 327
`
`

`

` 
`
` 
`
`c) 
`
`d) 
`
`e) 
`
`f) 
`
`g) 
`
`claim 13 further comprising at least two
`stepper motors mounted on the support
`structure, the stepper motors being operative
`to move the shuttle in at least two
`dimensions. ..................................................................... 74 
`[Claim 16] The thermal cycler apparatus of
`claim 13 further comprising a fitting on an
`exterior surface of the housing of the
`detection module, the fitting adapted to
`attach the detection module to the shuttle,
`wherein the fitting provides only electrical
`and mechanical connections. .......................................... 75 
`[Claim 17] The thermal cycler apparatus of
`claim 13 wherein the detection module
`includes at least two emission light
`detectors. ......................................................................... 75 
`[Claim 18] The thermal cycler apparatus of
`claim 13 wherein the detection module
`includes at least two excitation light
`generators. ....................................................................... 75 
`[Claim 19] The thermal cycler apparatus of
`claim 13 wherein movement of the shuttle
`and operation of the excitation light
`generator and the emission light detector are
`controlled by an external computer such that
`emission light is measured while the shuttle
`is in motion. .................................................................... 75 
`[Claim 20] The thermal cycler apparatus of
`claim 13 further comprising: a plurality of
`optical components defining an excitation
`optical path for light of an excitation
`wavelength from the excitation light
`generator to the opening of the housing and
`a detection optical path for light of a
`detection wavelength from the opening of
`the housing to the emission light detector,
`wherein all of the optical components of the
`excitation optical path and the detection
`optical path are disposed within the housing
`‐vii-
`
`Agilent Exhibit 1208
`Page 7 of 327
`
`

`

` 
`
` 
`
`h) 
`
`B. 
`
`C. 
`
`of the detection module. ................................................. 76 
`[Claim 22] The thermal cycler apparatus of
`claim 13 wherein the plurality of transparent
`portions of the heater includes a plurality of
`holes extending through the heater and
`aligned with the sample wells. ........................................ 76 
`Ground II: Claim 21 would have been obvious over
`Kordunsky ............................................................................................ 77 
`Ground III: Claim 21 would have been obvious over
`Kordunsky in view of Li ...................................................................... 78 
`D.  Ground IV: Claims 1-10, 13-16 and 19-22 Would
`Have Been Obvious Over Li in View of Brown in
`Further View of Bjornson ................................................................... 82 
`1. 
`Independent claim 1 .................................................................. 86 
`a) 
`[Element 1pre] - “A fluorescence detection
`apparatus for analyzing samples located in a
`plurality of wells in a thermal cycler, the
`apparatus comprising” .................................................... 86 
`[Element 1a] - “a support structure
`attachable to the thermal cycler; and” ............................ 86 
`[Element 1b] - “a shuttle movably mounted
`on the support structure” ................................................. 86 
`[Element 1c] - “a detection module attached
`to the shuttle, the detection module
`including:” ...................................................................... 87 
`[Element 1d] - “a housing having an
`opening oriented toward the plurality of
`wells” .............................................................................. 88 
`[Element 1e] - “an excitation light generator
`disposed within the housing ........................................... 88 
`[Element 1f] - “an emission light detector
`disposed within the housing,” ......................................... 88 
`[Element 1g] - “wherein, when the support
`structure is attached to the thermal cycler,” ................... 89 
`[Element 1h] - “a heating element is
`‐viii-
`
`b) 
`
`c) 
`
`d) 
`
`e) 
`
`f) 
`
`g) 
`
`h) 
`
`i) 
`
`Agilent Exhibit 1208
`Page 8 of 327
`
`

`

`2. 
`
` 
`
` 
`
`j) 
`
`k) 
`
`b) 
`
`disposed between the detection module and
`the sample wells and” ..................................................... 89 
`[Element 1i] - “the shuttle is movable to
`position the detection module in optical
`communication with different wells of the
`plurality of wells” ........................................................... 90 
`[Element 1j] - “through a plurality of
`openings extending through the heating
`element.” ......................................................................... 91 
`Dependent Claims 2-12 ............................................................. 91 
`a) 
`[Claim 2] “The fluorescence detection
`apparatus of claim 1 wherein an excitation
`optical path from the excitation light
`generator to the opening has a fixed length
`and a detection optical path from the
`opening to the emission light detector has a
`fixed length.” .................................................................. 92 
`[Claim 3] The fluorescence detection
`apparatus of claim 1 further comprising: a
`calibration element disposed such that the
`detection module is movable so as to be
`positioned in optical communication with
`the calibration element, wherein the
`calibration element provides a known
`fluorescence response.” .................................................. 92 
`[Claim 4] The fluorescence detection
`apparatus of claim 3 wherein the calibration
`element is located between two or more of
`the plurality of wells.” .................................................... 93 
`[Claim 5] The fluorescence detection
`apparatus of claim 3 wherein the calibration
`element is located in an area peripheral to
`the plurality of wells. ...................................................... 93 
`[Claim 6] The fluorescence detection
`apparatus of claim 1 wherein positioning of
`the detection module with respect to the
`wells is controlled by an external computer. .................. 93 
`[Claim 7] The fluorescence detection
`‐ix-
`
`c) 
`
`d) 
`
`e) 
`
`f) 
`
`Agilent Exhibit 1208
`Page 9 of 327
`
`

`

` 
`
` 
`
`3. 
`
`g) 
`
`h) 
`
`i) 
`
`apparatus of claim 1 wherein operation of
`the excitation light generator and the
`emission light detector is controlled by an
`external computer. .......................................................... 94 
`[Claim 8] “The fluorescence detection
`apparatus of claim 1 wherein the detection
`module is detachably attached to the
`shuttle.” ........................................................................... 94 
`[Claim 9] The fluorescence detection
`apparatus of claim 1 wherein the excitation
`light generator comprises a light-emitting
`diode. .............................................................................. 97 
`[Claim 10] The fluorescence detection
`apparatus of claim 1 further comprising at
`least two stepper motors mounted on the
`support structure, the stepper motors being
`operative to move the shuttle in at least two
`dimensions. ..................................................................... 98 
`Independent Claim 13 ............................................................... 99 
`a) 
`[Element 13pre] - “A thermal cycler
`apparatus comprising:” ................................................... 99 
`[Element 13a] - “a thermal cycler having an
`exterior housing and a plurality of sample
`wells for holding reaction vessels” ................................. 99 
`[Element 13b] - “a heater to prevent
`condensation from forming on a surface of
`the reaction vessels when the reaction
`vessels are in the sample wells,” .................................... 99 
`[Element 13c] - “the heater having a
`plurality of transparent portions to permit
`optical communication with each of the
`plurality sample wells;” ................................................ 100 
`[Element 13d] - “a support structure
`disposed inside the exterior housing on an
`opposite side of the heater from the sample
`wells” ............................................................................ 100 
`[Element 13e] - “a shuttle movably mounted
`‐x-
`
`b) 
`
`c) 
`
`d) 
`
`e) 
`
`f) 
`
`Agilent Exhibit 1208
`Page 10 of 327
`
`

`

` 
`
` 
`
`4. 
`
`g) 
`
`h) 
`
`i) 
`
`j) 
`
`k) 
`
`on the support structure; and” ....................................... 101 
`[Element 13f] - “a detection module
`attached to the shuttle, the detection module
`including: a module housing having an
`opening that is oriented toward the plurality
`of sample wells when the thermal cycler is
`in an operating state;” ................................................... 101 
`[Element 13g] - “an excitation light
`generator disposed entirely within the
`module housing; and” ................................................... 101 
`[Element 13h] - “an emission light detector
`disposed entirely within the module
`housing;” ....................................................................... 102 
`[Element 13i] - “when the thermal cycler is
`in the operating state, the shuttle is movable
`to position the detection module in optical
`communication with different sample wells
`of the plurality of sample wells” .................................. 102 
`[Element 13j] - “through the transparent
`portions of the heater.” ................................................. 102 
`Dependent claims 14-16 and 19-22 ........................................102 
`a) 
`[Claim 14] “The thermal cycler apparatus of
`claim 13 wherein the excitation light
`generator comprises a light-emitting diode.” ............... 103 
`[Claim 15] “The thermal cycler apparatus of
`claim 13 further comprising at least two
`stepper motors mounted on the support
`structure, the stepper motors being operative
`to move the shuttle in at least two
`dimensions.” ................................................................. 103 
`[Claim 16] “The thermal cycler apparatus
`of claim 13 further comprising a fitting on
`an exterior surface of the housing of the
`detection module, the fitting adapted to
`attach the detection module to the shuttle,
`wherein the fitting provides only electrical
`and mechanical connections.” ...................................... 103 
`
`b) 
`
`c) 
`
`‐xi-
`
`Agilent Exhibit 1208
`Page 11 of 327
`
`

`

` 
`
` 
`
`E. 
`
`d) 
`
`e) 
`
`[Claim 19] “The thermal cycler apparatus of
`claim 13 wherein movement of the shuttle
`and operation of the excitation light
`generator and the emission light detector are
`controlled by an external computer such that
`emission light is measured while the shuttle
`is in motion.” ................................................................ 105 
`[Claim 20] “The thermal cycler apparatus of
`claim 13 further comprising: a plurality of
`optical components defining an excitation
`optical path for light of an excitation
`wavelength from the excitation light
`generator to the opening of the housing and
`a detection optical path for light of a
`detection wavelength from the opening of
`the housing to the emission light detector,
`wherein all of the optical components of the
`excitation optical path and the detection
`optical path are disposed within the housing
`of the detection module.” .............................................. 106 
`[Claim 21] “The thermal cycler apparatus of
`claim 13 wherein the detection module is
`positioned such that the opening is below
`the plurality of sample wells.” ...................................... 106 
`[Claim 22] “The thermal cycler apparatus of
`claim 13 wherein the plurality of transparent
`portions of the heater includes a plurality of
`holes extending through the heater and
`aligned with the sample wells.” .................................... 107 
`Ground V: Claims 11-12 and 17-18 Would Have Been
`Obvious Over Li in View of Brown, in Further View of
`Bjornson and Miller........................................................................... 107 
`1. 
`[Claim 11] The fluorescence detection apparatus
`of claim 1 wherein the detection module includes
`at least two emission light detectors. ......................................110 
`[Claim 12] The fluorescence detection apparatus
`of claim 1 wherein the detection module includes
`at least two excitation light generators. ..................................110 
`‐xii-
`
`f) 
`
`g) 
`
`2. 
`
`Agilent Exhibit 1208
`Page 12 of 327
`
`

`

` 
`
`F. 
`
`3. 
`
`4. 
`
`[Claim 17] “The thermal cycler apparatus of
`claim 13 wherein the detection module includes
`at least two emission light detectors.” ....................................110 
`[Claim 18] “The thermal cycler apparatus of
`claim 13 wherein the detection module includes
`at least two excitation light generators.” .................................110 
`Ground VI: Claim 19 Would Have Been Obvious Over
`of Li in View of Brown and Bjornson, in Further View
`of Heffelfinger ................................................................................... 110 
`1. 
`[Claim 19] “The thermal cycler apparatus of
`claim 13 wherein movement of the shuttle and
`operation of the excitation light generator and
`the emission light detector are controlled by an
`external computer such that emission light is
`measured while the shuttle is in motion.” ...............................112 
`XIII.  CONCLUSION ......................................................................................... 113 
`
` 
`
`
` 
`
`
`
`‐xiii-
`
`Agilent Exhibit 1208
`Page 13 of 327
`
`

`

` 
`
`
`APPENDIX A
`
`
`
` 
`
` 
`
`LIST OF APPENDICIES
`
`
`
`Claim Charts
`
`
`
`‐xiv-
`
`Agilent Exhibit 1208
`Page 14 of 327
`
`

`

`Exhibit List
`
`1201 Kordunsky, et al., U.S. Patent No. 8,236,504, “Systems and methods
`for fluorescence detection with a movable detection module” (Filed:
`Jun. 30, 2010; Issued: Aug. 7, 2012)
`
`1202 Kordunsky et al., U.S. Patent No. 7,148,043, “Systems and methods
`for fluorescence detection with a movable detection module” (Filed:
`May 8, 2003; Issued: December 12, 2006)
`
`1203 Kordunsky, et al., U.S. Patent No. 8,835,118, “Systems and methods
`for fluorescence detection with a movable detection module” (Filed:
`Jul. 5, 2012; Issued: Sept. 16, 2014)
`
`1204 Kordunsky, et al., U.S. Patent Application No. 10/431,708 filed May
`8, 2003, “Systems and methods for fluorescence detection with a
`movable detection module” (File History for U.S. Patent No.
`7,148,043)
`
`1205 Kordunsky, et al., U.S. Patent Application No. 12/827,521, filed Jun.
`30, 2010, “Systems and methods for fluorescence detection with a
`movable detection module” (File History for U.S. Patent No.
`8,236,504)
`
`1206 Kordunsky, et al., U.S. Patent Application No. 13/542,587, filed Jul.
`5, 2012, “Systems and methods for fluorescence detection with a
`movable detection module” (File History for U.S. Patent No.
`8,835,118)
`
`1207 Kordunsky et al., U.S. Patent Application No. 11/555,642 filed Nov.
`1, 2006 “Systems and methods for fluorescence detection with a
`movable detection module” (File History for U.S. Patent No.
`7,749,736)
`
`1208 Declaration of Professor James P. Landers
`
`1209
`
`Curriculum Vitae of Professor James P. Landers
`
`1210 Kordunsky, et al., International Publication No. WO2004/104547,
`“Systems and methods for fluorescence detection with a movable
`detection module” (Filed: May 10, 2004; Published: Dec. 2, 2004)
`‐xv-
`
`Agilent Exhibit 1208
`Page 15 of 327
`
`

`

`Exhibit List
`
`1211
`
`1212
`
`Li, et al., Chinese Publication No. CN1379236, “Fluorescence
`quantitative PCR analyzing system” (Filed: Apr. 12, 2001; Published:
`Nov. 13, 2002)
`
`Certified Translation of Li, et al., “Fluorescence quantitative PCR
`analyzing system” Chinese Publication No. CN1379236 (Filed: Apr.
`12, 2001; Published: Nov. 13, 2002) (Certification Document
`Included)
`
`1213 Miller, et al., U.S. Patent No. 5,528,050, “Compact scan head with
`multiple scanning modalities” (Filed: Jul. 24, 1995; Issued: Jun. 18,
`1996)
`
`1214
`
`1215
`
`1216
`
`1217
`
`1218
`
`Randrianarivo, et al., U.S. Patent No. 6,630,108, “Optical Measuring
`Head, in Particular For Automatic Chemical or Biological Reaction
`Analyzer” (Filed Oct. 4, 1999, Issued Oct. 7, 2003)
`
`Randrianarivo, et al., PCT WO00/22418, “Optical Measuring Head,
`in Particular For Automatic Chemical or Biological Reaction
`Analyzer”
`
`Certified Translation of Randrianarivo, et al., PCT WO00/22418,
`“Optical Measuring Head, in Particular For Automatic Chemical or
`Biological Reaction Analyzer”
`
`Bjornson, et al., U.S. Patent No. 5,125,748, “Optical Detection
`Module for Use in an Automated Laboratory Work Station” (Filed:
`May 10, 1991; Issued Jun. 30, 1992)
`
`Brown, et al., U.S. Patent No. 6,730,883, “Flexible heating cover
`assembly for thermal cycling of samples of biological material” (Filed
`Oct. 2, 2002; Issued May 4, 2004)
`
`1219 Heffelfinger, et al., U.S. Patent 5,784,152, “Tunable excitation and/or
`tunable detection microplate reader” (Filed: Oct. 11, 1996; Issued:
`Jul. 21, 1998)
`
`1220 Heffelfinger, et al., U.S. Patent No. 6,043,506, “Multi parameter
`scanner” (Filed: Dec. 30, 1997; Issued: Mar. 28, 2000)
`
`‐xvi-
`
`Agilent Exhibit 1208
`Page 16 of 327
`
`

`

`1221
`
`Exhibit List
`Pantoliano, et al., U.S. Patent 6,303,322, “Method for identifying lead
`compound” (Filed: Jan. 5, 2000; Issued: Oct. 16, 2001)
`
`1222 Gambini, et al., PCT Publication WO 99/60381, “Instrument for
`Monitoring Polymerase Chain Reaction of DNA,” (Filed: May 17,
`1999; Published Nov. 25, 1999)
`
`1223
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`1224
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`1225
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`1226
`
`Iwasaki, et al., Japanese Publication No. JP2001-242081, “DNA chip
`reading head and DNA chip reader” (Filed: Feb. 29, 2000; Published:
`Sep. 7, 2001)
`
`Certified Translation of Iwasaki, et al., Japanese Publication No.
`JP2001-242081, “DNA chip reading head and DNA chip reader”
`Japanese Publication No. JP2001-242081 (Filed: Feb. 29, 2000;
`Published: Sep. 7, 2001) (Certification Document Included)
`
`Fitzgerald, D., “Up to Speed on PCR” The Scientist, Vol. 14, Issue
`23, pp. 31-33 (Nov. 27, 2000)
`
`Lauermann, L., “Advances in PCR Technology” Animal Health
`Research Reviews, Vol. 5, Issue 2, pp. 247-248 (Dec. 2004)
`
`1227 Meisenholder, G., “New Lids on the Block - LabConsumer Annual
`Review of Thermal Cyclers” The Scientist, Vol. 13, Issue 23, pp. 17-
`19 (Nov. 22, 1999)
`
`1228
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`1230
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`S. Back, “A Full Plate: UV/Vis Microplate Readers” The Scientist
`(July 6, 1998), available at https://www.the-scientist.com/technology-
`profile/a-full-plate-uvvis-microplate-readers-56929
`
`Bouma, et al, U.S. Patent No. 5,585,242, “Method for Detection of
`Nucleic Acid Using Total Internal Reflectance” (Filed: Aug. 31,
`1995; Issued Dec. 17, 1996)
`
`Ririe, et al., International Publication No. WO03/007677, “Thermal
`cycling system and method of use” (Filed: Jul. 16, 2002; Published:
`Jan. 30, 2003)
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`1231
`
`Reid, et al., U.S. Publication No. 2003/0106682, “Side-wall heater for
`thermocycler device” (Filed: Oct. 1, 2002; Published: Jun. 12, 2003)
`
`‐xvii-
`
`Agilent Exhibit 1208
`Page 17 of 327
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`

`

`Exhibit List
`
`1232
`
`Ackley, H., U.S. Patent 6,003,775, “Generic handheld symbology
`scanner with modular optical sensor” (Filed: Jun. 11, 1997; Issued:
`Dec. 21, 1999)
`
`1233 Kawakami, H., U.S. Patent 6,520,625, “Carriage and recording
`apparatus” (Filed: Aug. 17, 2000; Issued: Feb. 18, 2003)
`
`1234
`
`Constans, A., “Some Like It Hot: A Thermal Cycler Roundup” The
`Scientist, Vol. 15, Issue 24, pp. 32-35 (Dec. 10, 2001)
`
`1235 Mitoma, et al., U.S. Patent No. 6,144, 448 “Fluorescense detecting
`apparatus” (Filed: Oct. 30, 1996; Issued: Nov. 7, 2000)
`
`1236 Vuong, U.S. Patent No. 6,448,089, “Multiwell Scanner and Scanning
`Method” (Filed Oct. 12, 1999, Issued Sept. 10, 2002)
`
`1237 Oldham, et al., International Publication No. WO02/06796,
`“Scanning system and method for scanning a plurality of samples”
`(Filed: Jul. 13, 2001; Published: Jan. 24, 2002)
`
`1238 Wittwer, et al., U.S. Patent 6,140,054, “Multiplex genotyping using
`fluorescent hybridization probes” (Filed: Sep. 30, 1998; Issued: Oct.
`31, 2000)
`
`1239
`
`Berndt, et al., U.S. Patent 5,595,708, “System for detecting bacterial
`growth in a plurality of culture vials” (Filed: Nov. 18, 1994; Issued:
`Jan. 21, 1997)
`
`1240 Giebeler, et al., U.S. Patent 6,316,774, “Optical system for a scanning
`fluorometer” (Filed: Mar. 23, 1999; Issued: Nov. 13, 2001)
`
`1241
`
`Fernandes, et al., U.S. Patent 5,436,718, “Mutli[sic.]-functional
`photometer with movable linkage for routing optical fibers” (Filed:
`Jul. 30, 1993; Issued: Jul. 25, 1995)
`
`1242 Diggle, et al., “Automation of Fluorescence-Based PCR for
`Confirmation of Meningococcal Disease” Journal of Clinical
`Microbiology, Vol. 39, No. 12, 2001, pp. 4518-4519
`
`‐xviii-
`
`Agilent Exhibit 1208
`Page 18 of 327
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`

`

`Exhibit List
`
`1243 Higuchi, et al., European Patent No. EP0640828, “Monitoring
`multiple reactions simultaneously and analyzing same” (Filed: Aug.
`16, 1994; Issued: Jun. 10, 2000)
`
`1244 Woudenberg, et al., U.S. Patent No. 5,928,907, “System for real time
`detection of nucleic acid amplification products” (Filed: Dec. 2, 1996;
`Issued: Jul. 27, 1999)
`
`1245
`
`1246
`
`1247
`
`1248
`
`1249
`
`1250
`
`1251
`
`1252
`
`1253
`
`IPR2017-00054, -00055, Transcript of Deposition of Dean Neikirk,
`Ph.D. conducted August 22, 2017
`
`IPR2017-00054, Petition (Paper No. 3)
`
`IPR2017-00054 Declaration of Richard Mathies, Ph.D. (Thermo
`Fisher Ex. 1002)
`
`IPR2017-00054 Preliminary Patent Owner Response (Paper No. 7,
`Jan. 19, 2017)
`
`IPR2017-00054 Institution Decision (Paper No. 8, Mar. 17, 2017)
`
`IPR2017-0