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`PATENT ABSTRACTS OF JAPAN
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`(11)Publication number :
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`04-057853
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`(43)Date of publication of application : 25.02.1992
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`(21)Application number: 02-170549
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`(22)Dateoffiling :
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`28.06.1990
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`(71)Applicant : CHLORINE ENG CORP LTD
`ITANO REITOU KK
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`(72)Inventor : TOKUMORI TSUNEO
`SUMIDA YOKO
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`TSUYAMA KOICHI
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`KUNISHIRO IYOKO
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`OKADA HARUO
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`TANI TOSHIFUMI
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`(54) METHOD FOR EXTRACTING AND SEPARATING COLORING MATTER FROM KRILL
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`(57)Abstract:
`PURPOSE: To prepare a reddish orange coloring matter
`having a high safety in a high concn. by extracting, with
`CO2 in a supercritical state, krill shells of which the
`protein has been decomposedbya protease.
`CONSTITUTION: Krill shells are treated with a protease
`to decomposethe protein in the shells and the treatment
`productis filtered. The residue offiltration is dried to give
`treated shells having a water content of 6-8% and a
`mean particle size of 200 um or lower. The treated shells
`are put into an extraction vessel 5. An extractant
`comprising a lig. CO2 in an amt. of 30-40 pts.wt. based
`on one pt.wt. treated shells having a coloring matter
`concn. of 30 mg/100 g is supplied through a
`supercooling apparatus 2 to a pump 3, pressurized at
`the pump 3 to 100-250 kg/cm2, heated with a heat exchanger 4 to 35-40°C to bring it into a
`supercritical state, and transferred to the extraction vessel 5 to extract an oil in the treated
`shells. After the pressure of the oil-contg. CO2 in the supercritical state is reduced to 40-60
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`http://www 19.ipdL.inpit.go.jp/PA 1/result/detail/main/wAAAw7aOhjDA404057853P1.hbtm
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`1/24/2011
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`AKER EXHIBIT 2014 Page 1
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`AKER EXHIBIT 2014 Page 1
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`kg/cm2 with a pressure reducing valve 6, the CO2is delivered through a selector valve 11 to
`the first separating vessel 7 to separate the oil, and recycled through a selector valve 13, a
`pressure reducing valve 9, a condense 10, a water separator 15, and a storage vessel 1 to
`the extraction vessel 5. Then, selector valves 11 and 13 are closed while selector valves 12
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`and 14 are opened, and the CO2 contg. the coloring matteris transferred to the second
`separating vessel 8, where the CO2 is evaporated to give a coloring matter with a concn. of
`2000-10000 mg/100g.
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`http://www 19.ipdL.inpit.go.jp/PA 1/result/detail/main/wAAAw7aOhjDA404057853P1.hbtm
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