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`PATENT ABSTRACTS OF JAPAN
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`(11)Publication number:
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`04-057853
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`(43)Date of publication of application : 25.02.1992
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`(21)Application number : 02-170549
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`(71)Applicant : CHLORINE ENG CORP LTD
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`|TANO REITOU KK
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`(22)Date of filing :
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`28.06.1990
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`(72)lnventor : TOKUMORI TSUNEO
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`SUMIDA YOKO
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`TSUYAMA KOICHI
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`KUNISHIRO lYOKO
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`OKADA HARUO
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`TANI TOSHIFUMI
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`(54) METHOD FOR EXTRACTING AND SEPARATING COLORING MATTER FROM KRILL
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`(57)Abstract:
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`PURPOSE: To prepare a reddish orange coloring matter
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`having a high safety in a high concn. by extracting, with
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`CO2 in a supercritical state, krill shells of which the
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`protein has been decomposed by a protease.
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`CONSTITUTION: Krill shells are treated with a protease
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`to decompose the protein in the shells and the treatment
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`product is filtered. The residue of filtration is dried to give
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`treated shells having a water content of 6—8% and a
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`mean particle size of 200 pm or lower. The treated shells
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`are put into an extraction vessel 5. An extractant
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`comprising a liq. CO2 in an amt. of 30-40 pts.wt. based
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`on one pt.wt. treated shells having a coloring matter
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`concn. of 30 mg/100 g is supplied through a
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`supercooling apparatus 2 to a pump 3, pressurized at
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`
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`the pump 3 to 100-250 kg/cm2, heated with a heat exchanger 4 to 35-40°C to bring it into a
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`supercritical state, and transferred to the extraction vessel 5 to extract an oil in the treated
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`shells. After the pressure of the oil-contg. 002 in the supercritical state is reduced to 40-60
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`http://wwwl 9.ipd1.inpit.go.jp/PA1/resu1t/detai1/rnain/WAAAW7aOhjDA404057853P1 .htrn
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`AKER EXHIBIT 2014 Page 1
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`AKER EXHIBIT 2014 Page 1
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`kg/cm2 with a pressure reducing valve 6, the CO2 is delivered through a selector valve 11 to
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`the first separating vessel 7 to separate the oil, and recycled through a selector valve 13, a
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`pressure reducing valve 9, a condense 10, a water separator 15, and a storage vessel 1 to
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`the extraction vessel 5. Then, selector valves 11 and 13 are closed while selector valves 12
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`and 14 are opened, and the CO2 contg. the coloring matter is transferred to the second
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`separating vessel 8, where the CO2 is evaporated to give a coloring matter with a concn. of
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`2000-10000 mg/100g.
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`http://wwwl 9.ipd1.inpit.go.jp/PA1/resu1t/detai1/rnain/WAAAW7aOhjDA404057853P1 .htrn
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`AKER EXHIBIT 2014 Page 2
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`AKER EXHIBIT 2014 Page 2
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