Curriculum vitae
`
`ALEXANDER M. KLIBANOV
`
`Date and Place of Birth:
`
`July 15, 1949, in Moscow (Russia)
`
`Nationality:
`
`Education:
`1974
`1971
`
`Naturalized U.S. Citizen (1983)
`
`Ph.D. in Chemical Enzymology, Moscow University
`M.S. in Chemistry, Moscow University
`
`2004
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`2001
`2001
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`2000
`2000
`1998
`1996
`1995
`1995
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`Honors:
`2015
`Tau Beta Pi’s Leonardo Da Vinci Lecturer, MIT
`2007-11 and 2014- Novartis Chair Endowed Professorship, MIT
`Roger and Georges Firmenich Endowed Professorship, MIT
`2012-13
`MIT Biological Engineering Senior Class Faculty Award
`2011
`Distinguished GRUM Lecturer in Drug Discovery & Development,
`2006
`University of Montreal (Canada)
`UNAM Distinguished Lecturer, National University of Mexico (Mexico
`City)
`Walter Enz Lecturer in Pharmaceutical Chemistry, University of Kansas
`Elected a Corresponding Fellow of the Royal Society of Edinburgh
`(Scotland’s National Academy of Science and Letters)
`Merck Distinguished Lecturer, Rutgers University
`Top 20 Biotechnology & Bioengineering Papers of the Last Forty Years
`Robert Lutz Lecturer, University of Virginia
`Perkin-Elmer Distinguished Lecturer, University of Pittsburgh
`Elected to the National Academy of Sciences of the U.S.A.
`Nathan O. Kaplan Memorial Lecturer in Biological Chemistry, University
`of California at San Diego
`R.W. Johnson PRI Lecturer, Pharmaceutical Research Institute
`Warren McCabe Lecturer, North Carolina State University
`Elected to the National Academy of Engineering of the U.S.A.
`Arthur C. Cope Scholar Award of the American Chemical Society
`Biotechnology Divisional Lectureship Award of the Institute of Food
`Technologists
`Charles Sabat Lecturer, Rutgers University
`Elected a Founding Fellow of the American Institute for Medical and
`Biological Engineering
`Louis C. Jordy Research Scholar Lecturer, Drew University
`International Enzyme Engineering Award
`Marvin J. Johnson Award of the American Chemical Society
`Monsanto Lecturer, Ohio State University
`N RC Distinguished Lecturer, Academia Sinica (Taiwan)
`I patieff Prize of the American Chemical Society
`Backer Lecturer, Groningen University (Holland)
`Dow Lecturer, University of Ottawa (Canada)
`Distinguished Scholar Lecturer, Hope College
`Leo Friend Award of the American Chemical Society
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`1994
`1994
`1993
`1993
`1993
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`1992
`1992
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`1992
`1991
`1991
`1990
`1990
`1989
`1989
`1988
`1987
`1986
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`Almirall EXHIBIT 2004
`Amneal v. Almirall
`IPR20I 8-00608
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`1984
`1984
`1982
`1981-1983
`1975
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`Who’s Who in Frontier Science and Technology
`Sohio Lecturer, Case Western Reserve University
`American Men and Women of Science
`Henry L. Doherty Career Development Professorship, MIT
`U.S.S.R. Ministry of Higher Education Prize
`
`Professional Experience:
`2007-11 and 2014- Novartis Chair Endowed Professor of Chemistry and Bioengineering, MIT
`
`2012-2013
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`2000-present
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`1988-present
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`1987-1988
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`1983-1987
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`1979-1983
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`1977-1979
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`1974-1977
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`Roger and Georges Firmenich Professor of Natural Products Chemistry
`Department of Chemistry, MIT
`
`Professor of Bioengineering
`Department of Biological Engineering, MIT
`
`Professor of Chemistry
`Department of Chemistry, MIT
`
`Professor of Applied Biochemistry
`Department of Applied Biological Sciences, MIT
`
`Associate Professor of Applied Biochemistry
`Department of Applied Biological Sciences (formerly
`Department of Nutrition and Food Science), MIT
`
`Assistant Professor of Applied Biochemistry
`Department of Nutrition and Food Science, MIT
`
`Postdoctoral Associate, Department of Chemistry
`University of California at San Diego
`
`Research Chemist
`Department of Chemistry, Moscow University
`
`Current Journal Editorial/Advisory Boards:
`"Biocatalysis and Biotransformation", "Applied Biochemistry and Biotechnology", "Open
`Chemistry Journal", "Biotechnology Progress", "Biotechnology & Bioengineering", "Microbial
`Biotechnology", "Open Journal of Pharmacology", "Nanocarriers", "Journal of Antivirals and
`Antiretrovirals", "Open Access Academic Books in Chemistry", "Journal of Biological Chemistry
`and Molecular Pharmacology", "Archives of Natural Products and Medicinal Chemistry", "Recent
`Patents in Biotechnology", "Current Pharmaceutical Biotechnology", and "Archives of Medical
`Biotechnology", "International Journal of Drug Design, Delivery and Safety"
`
`Professional Societies:
`U.S. National Academy of Sciences, U.S. National Academy of Engineering, American
`Chemical Society, American Institute for Medical and Biological Engineering
`
`Current Research Interests:
`Medicinal and formulation chemistry
`Enzyme chemistry and biotechnology
`Antimicrobial polymers
`Protein drug delivery
`Enzymes as stereoselective catalysts in organic syntheses
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`Stabilization and formulation of macromolecular pharmaceuticals
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`Publications:
`1. Varfolomeyev, S.D., Klibanov, A.M., Berezin, I.V. 1971. Light-initiated enzymic activity
`caused by photostereoisomerization of cis-4-nitrocinnamoyl-a-chymotrypsin. FEBS Lett. 15:
`118 -120.
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`2.
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`3.
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`4.
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`5.
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`6.
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`7.
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`8.
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`9.
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`Varfolomeyev, S.D., Klibanov, A.M., Martinek, K., Berezin, I.V. 1972. Light-sensitive catalysts.
`4-Nitrocinnamoyl residue as a chromophoric reporter group in the a-chymotrypsin active center.
`Dokl. Acad. Nauk SSSR 203: 616-619.
`
`Klibanov, A.M., Samokhin, G.P., Martinek, K., Berezin, I.V. 1974. Mechanochemistry of
`catalytic systems. Regulation by a mechanical action of the enzymatic properties of
`a-chymotrypsin covalently attached to a nylon fiber. Dokl. Acad. Nauk SSSR 218: 715-718.
`
`Berezin, I.V., Varfolomeyev, S.D., Klibanov, A.M., Martinek, K. 1974. Light and ultrasonic
`regulation of a-chymotrypsin catalytic activity. Proflavin as a light- and sound- sensitive
`competitive inhibitor. FEBS Lett. 39: 329-331.
`
`Berezin, I.V., Klibanov, A.M., Martinek, K. 1974. The mechanochemistry of immobilized
`enzymes. How to steer a chemical process at the molecular level by a mechanical device.
`Biochim. Biophys. Acta 364: 193-199.
`
`Berezin, I.V., Klibanov, A.M., Goldmacher, V.S., Martinek, K. 1974. Mechanochemistry of
`catalytic systems. Regulation by a mechanical action of the enzymatic activity of trypsin
`entrapped in polyacrylamide gel. Dokl. Acad. Nauk SSSR 218: 367-370.
`
`Klibanov, A.M., Martinek, K., Berezin, I.V. 1974. The effect of ultrasound on a-chymotrypsin.
`A novel approach to studying conformational transitions in active centers of enzymes.
`Biochemistry SSSR 39: 878-887.
`
`Berezin, I.V., Klibanov, A.M., Klyosov, A.A., Martinek, K., Svedas, V.K. 1975. The effect of
`ultrasound as a new method of studying conformational transitions in enzyme active centers.
`pH- and temperature-induced conformational transitions in the active center of penicillin
`amidase. FEBS Lett. 49: 325-328.
`
`Berezin, I.V., Klibanov, A.M., Martinek, K. 1975. Kinetic and thermodynamic aspects of
`catalysis by immobilized enzymes. Russ. Chem. Revs. 4.~4: 17-47.
`
`10. Martinek, K., Goldmacher, V.S., Klibanov, A.M., Berezin, I.V. 1975. Denaturing agents (urea,
`acrylamide) protect enzymes against irreversible thermoinactivation: a study with native and
`immobilized a-chymotrypsin and trypsin. FEBS Lett. 51 152-155.
`
`11. Martinek, K., Klibanov, A.M., Tchernysheva, A.V., Berezin, I.V. 1975. The stabilization of
`a-chymotrypsin by entrapment in polymethacrylate gels. Dokl. Acad. Nauk SSSR 223: 233-236.
`
`12.
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`Tchernysheva, A.V., Goldmacher, V.S., Klibanov, A.M., Martinek, K., Berezin, I.V. 1975. The
`catalytic activity and thermostability of a-chymotrypsin oligomers entrapped in cross-linked
`polymeric gels. Bull. Moscow Univ. 1.~9: 428-431.
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`13.
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`14.
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`15.
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`16.
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`17.
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`Tchernysheva, A.V., Martinek, K., Klibanov, A.M., Mevkh, A.T., Berezin, I.V. 1975. The
`catalytic properties and thermostability of a-chymotrypsin in different polymethacrylate gels.
`Izvestia Acad. Nauk SSSR, Ser. Khim. No.8: 1764-1768.
`
`Klibanov, A.M., Samokhin, G.P., Martinek, K., Berezin, I.V. 1976. Enzymatic mechano-
`chemistry: a new approach to studying the mechanism of enzyme action. Biochim. Biophys.
`Acta 438: 1-12.
`
`Berezin, I.V., Klibanov, A.M., Samokhin, G.P., Martinek, K. 1976. Mechanochemistry of
`immobilized enzymes: a new approach to studies in fundamental enzymology. Regulation by a
`mechanical device of the catalytic properties of enzymes covalently attached to elastic
`polymeric supports. Meth. Enzymol. 44: 558-571.
`
`Berezin, I.V., Klibanov, A.M., Goldmacher, V.S., Martinek, K. 1976. Mechanochemistry of
`immobilized enzymes: a new approach to studies in fundamental enzymology. Regulation by a
`mechanical device of the catalytic activity of enzymes trapped in polyacrylamide gel. Meth.
`Enzymol. 44: 571-576.
`
`Klibanov, A.M., Kazanskaya, N.F., Larionova, N.I., Martinek, K., Berezin, I.V. 1976. A
`comparative study of the dynamic structures of the active centers of proteolytic enzymes by the
`ultrasonic method. The effect of ultrasound on a-trypsin, I~-trypsin, and trypsinogen. J. Bioor.q.
`Chem. (Russ.)2: 828-836.
`
`18. Martinek, K., Goldmacher, V.S., Klibanov, A.M., Torchilin, V.P., Smirnov, V.N., Chazov, E.I.,
`Berezin, I.V. 1976. Thermal stabilization of a-chymotrypsin by covalent attachment to a
`complementary surface of a polymeric matrix. Dokl. Acad. Nauk SSSR 228: 1468-1471.
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`19.
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`20.
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`Poglazov, B.F., Samokhin, G.P., Klibanov, A.M., Levitsky, D.I., Martinek, K., Berezin, I.V. 1977.
`Mechanochemistry of the myosin molecule. Dokl. Acad. Nauk SSSR 234: 482-485.
`
`Berezin, I.V., Klibanov, A.M., Samokhin, G.P., Goldmacher, V.S., Martinek, K. 1977.
`Mechanosensitive and sound-sensitive systems as chemical amplifiers of weak signals. In:
`Biomedical Applications of Immobilized Enzymes and Proteins (T.M.S. Chang, ed.), vol. 2, pp.
`237-251, Plenum Press, New York.
`
`21.
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`Klibanov, A.M., Samokhin, G.P., Martinek, K., Berezin, I.V. 1977. A new mechanochemical
`method of enzyme immobilization. Biotechnol. Bioenq. 19: 211-218.
`
`22. Martinek, K., Klibanov, A.M., Samokhin, G.P., Semenov, A.M., Berezin, I.V. 1977. Preparative
`enzymatic synthesis in biphasic water-organic systems. J. Bioor.q. Chem. (Russ.) 3: 696-702.
`
`23.
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`Klibanov, A.M., Samokhin, G.P., Martinek, K., Berezin, I.V. 1977. A new approach to
`preparative enzymatic synthesis. Biotechnol. Bioen.q. 19: 1351-1361.
`
`24. Martinek, K., Klibanov, A.M., Goldmacher, V.S., Berezin, I.V. 1977. The principles of enzyme
`stabilization. I. Increase in thermostability of enzymes covalently bound to a complementary
`surface of a polymeric support in a multipoint fashion. Biochim. Biophys. Acta 485: 1-12.
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`25. Martinek, K., Klibanov, A.M., Goldmacher, V.S., Tchernysheva, A.V., Mozhaev, V.V., Berezin,
`I.V., Glotov, B.O. 1977. The principles of enzyme stabilization. II. Increase in thermostability of
`enzymes as a result of multipoint non-covalent interactions with a polymeric support. Biochim.
`Biophys. Acta 485: 13-28.
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`26.
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`27.
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`28.
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`29.
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`30.
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`31.
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`32.
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`33.
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`34.
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`35.
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`36.
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`37.
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`38.
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`Torchilin, V.P., Maksimenko, A.V., Smirnov, V.N., Martinek, K., Klibanov, A.M., Berezin, I.V.
`1978. The principles of enzymes stabilization. III. The effect of the length of intramolecular
`cross-linking agents on the thermostability of enzymes. Biochim. Biophys. Acta 522: 277-283.
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`Samokhin, G.P., Klibanov, A.M., Martinek, K. 1978. Photochemical immobilization of enzymes.
`Bull. Moscow Univ. 1!9: 433-436.
`
`Poglazov, B.F., Samokhin, G.P., Klibanov, A.M., Levitsky, D.I., Martinek, K., Berezin, I.V. 1978.
`The effect of mechanical stretching of myosin rod part (fragment LMM + HMM S-2) on the
`ATPase activity of myosin. Biochim. Biophys. Acta 524: 245-253.
`
`Klibanov, A.M., Semenov, A.N., Samokhin, G.P., Martinek, K. 1978. Enzymatic reactions in
`water-organic solutions. The criterion for selecting optimal organic co-solvents. J. Bioor.q.
`Chem. (Russ.)4: 236-242.
`
`Sinitsyn, A.P., Klibanov, A.M., Klesov, A.A., Martinek, K. 1978. The dependence of stability of
`immobilized glycoamylase on the method of immobilization. Appl. Biochem. Microbiol. (Russ.)
`14: 236-242.
`
`Klibanov, A.M., Mozhaev, V.V. 1978. On the mechanism of irreversible thermoinactivation of
`enzymes and possibilities for reactivation of "irreversibly" inactivated enzymes. Biochem.
`Biophys. IRes. Commun. 8._33: 1012-1017.
`
`Klibanov, A.M., Kaplan, N.O., Kamen, M.D. 1978. A rationale for stabilization of oxygen-labile
`enzymes: application to a clostridial hydrogenase. Proc. Natl. Acad. Sci. USA 7,5: 3640-3643.
`
`Torchilin, V.P., Maksimenko, A.V., Smirnov, V.N., Berezin, I.V., Klibanov, A.M., Martinek, K.
`1979. The principles of enzyme stabilization. IV. Modification of "key" functional groups in the
`tertiary structure of proteins. Biochim. Biophys. Acta 567: 1-11.
`
`Klibanov, A.M., Kaplan, N.O., Kamen, M.D. 1979. Chelating agents protect hydrogenase
`against oxygen inactivation. Biochim. Biophys. Acta 547: 411-416.
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`Klibanov, A.M. 1979. Stabilization of enzymes by immobilization. Anal. Biochem. 93: 1-25.
`
`Klibanov, A.M., Kaplan, N.O., Kamen, M.D. 1980. Thermal stabilities of membrane-bound,
`solubilized and artificially immobilized hydrogenase from Chromatium vinosum. Arch. Biochem.
`Biophys. 199: 545-549.
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`Klibanov, A.M., Puglisi, A.V. 1980. The regeneration of coenzymes using immobilized
`hydrogenase. Biotechnol. Lett. 2: 445-450.
`
`Klibanov, A.M., Kaplan, N.O., Kamen, M.D. 1980. Approaches to stabilization of hydrogenase
`and nitrogenase against oxygen inactivation. In: Enzyme Engineering V (H.H. Weetall and G.P.
`IRoyer, eds.), pp.135-142, Plenum Press, New York.
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`39.
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`40.
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`41.
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`42.
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`43.
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`44.
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`45.
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`46.
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`Klibanov, A.M., Alberti, B.N., Morris, E.D., Felshin, L.M. 1980. Enzymatic removal of toxic
`phenols and anilines from waste waters. J. Appl. Biochem. 2: 414-421.
`
`Khan, S.M., Klibanov, A.M., Kaplan, N.O., Kamen, M.D. 1981. The effect of electron carriers
`and other ligands on oxygen stability of clostridial hydrogenase. Biochim. Biophys. Acta 659:
`457-465.
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`Klibanov, A.M., Morris, E.D. 1981. Horseradish peroxidase for the removal of carcinogenic
`aromatic amines from water. Enzyme Microb. Technol. 3: 119-122.
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`Klibanov, A.M., Huber, J. 1981. Application of immobilized hydrogenase for the de-tritiation of
`water. Biotechnol. Bioenq. 2._33: 1537-1551.
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`Klibanov, A.M., Barta, T.E. 1981. Protection of immobilized sulfhydryl groups against auto-
`oxidation by alterations in their microenvironment. Appl. Biochem. Biotechnol. 6: 201-207.
`
`Alberti, B.N., Klibanov, A.M. 1981. Enzymatic removal of dissolved aromatics from industrial
`aqueous effluents. Biotechnol. Bioenq. Symp. 1"1: 373-379.
`
`Klibanov, A.M., Berman, Z., Alberti, B.N. 1981. Preparative hydroxylation of aromatic
`compounds catalyzed by peroxidase. J. Am. Chem. Soc. 103: 6263-6264.
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`Klibanov, A.M., Alberti, B.N., Zale, S.E. 1982. Enzymatic synthesis of formic acid from H2 and
`CO2 and production of hydrogen from formic acid. Biotechnol. Bioenq. 2.~4: 25-36.
`
`47. Maneepun, S., Klibanov, A.M. 1982. Stabilization of microbial proteases against autolysis
`using acylation with dicarboxylic acid anhydrides. Biotechnol. Bioenq. 2.~4: 483-486.
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`48.
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`49.
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`50.
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`51.
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`52.
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`53.
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`54.
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`Klibanov, A.M., Giannousis, P.P. 1982. Geometrically specific oxidation of I~-arylacroleins
`catalyzed by xanthine oxidase: the preparative potential. Biotechnol. Lett. 4: 57-60.
`
`Alberti, B.N., Klibanov, A.M. 1982. Preparative production of hydroquinone from benzoquinone
`catalyzed by immobilized glucose oxidase. Enzyme Microb. Technol. 4: 47-49.
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`Klibanov, A.M., Siegel, E.H. 1982. Geometric specificity of porcine liver carboxyl esterase and
`its application for the production of cis arylacrylic esters. Enzyme Microb. Technol. 4: 172-174.
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`Klibanov, A.M., Giannousis, P.P. 1982. Geometric specificity of alcohol dehydrogenases and
`its potential for separation of trans and cis isomers of unsaturated aldehydes. Proc. Natl. Acad.
`Sci. USA 79: 3462-3465.
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`Klibanov, A.M., Weare, N.M. 1982. Oxygen inactivation and stabilization of Azotobacter
`nitrogenase. In: From Cyclotrones to Cytochromes (N.O. Kaplan and A.B. Robinson, eds.),
`pp. 509-516, Academic Press, New York.
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`Zale, S.E., Klibanov, A.M. 1982. Application of immobilized hydrogenase to H2 storage in
`concentrated solutions of methyl viologen. Appl. Biochem. Biotechnol. 7: 317-323.
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`Klibanov, A.M., Alberti, B.N., Marietta, M.A. 1982. Stereospecific oxidation of aliphatic alcohols
`catalyzed by galactose oxidase. Biochem. Biophys. IRes. Commun. 10.~8: 804-808.
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`55.
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`56.
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`57.
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`58.
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`Pungor, E., Klibanov, A.M., Cooney, C.L., Weaver, J.C. 1982. Continuous 2HHO
`determination in H20 solutions by a computer-controlled mass spectrometer. Biomed. Mass
`Spectrom. 9: 181--185.
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`Klibanov, A.M. 1983. Unconventional catalytic properties of conventional enzymes:
`applications in organic chemistry. In: The Biological Basis of New Developments in
`Biotechnology (A. Hollaender, A.I. Laskin, and P. Rogers, eds.), pp. 497-518, Plenum Press,
`New York.
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`Klibanov, A.M. 1983. Biotechnological potential of the enzyme hydrogenase. Process
`Biochem. 18: 13-16.
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`Pelsy, G., Klibanov, A.M. 1983. Remarkable positional (regio-) specificity of xanthine oxidase
`and some dehydrogenases in the reactions with substituted benzaldehydes. Biochim. Biophys.
`Acta 742: 352-357.
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`59. Klibanov, A.M. 1983. Stabilization of enzymes against thermal inactivation. Adv. Appl.
`Microbiol. 29: 1-28.
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`60.
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`61.
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`62.
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`63.
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`64.
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`65.
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`66.
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`67.
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`68.
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`Klibanov, A.M., Chendrasekhar, A., Alberti, B.N. 1983. Enzymatic separation of cis and trans
`isomers of alicyclic alcohols. Enzyme Microb. Technol. 5: 265-268.
`
`Klibanov, A.M. 1983. Immobilized enzymes and cells as practical catalysts. Science 219:
`722-727.
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`Pelsy, G., Klibanov, A.M. 1983. Preparative separation of a- and I~- naphthols catalyzed by
`immobilized sulfatase. Biotechnol. Bioenq. 2,5: 919-928.
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`Zale, S.E., Klibanov, A.M. 1983. On the role of reversible denaturation (unfolding) in the
`irreversible thermal inactivation of enzymes. Biotechnol. Bioenq. 2,5: 2221-2230.
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`Klibanov, A.M., Tu, T.-M., Scott, K.P. 1983. Peroxidase-catalyzed removal of phenols from
`coal conversion wastewaters. Science 221: 259-261.
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`Atlow, S.C., Bonadonna-Aparo, L., Klibanov, A.M. 1984. Dephenolization of industrial
`wastewaters catalyzed by polyphenol oxidase. Biotechnol. Bioenq. 2._66: 599-603.
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`Cambou, B., Klibanov, A.M. 1984. Preparative production of optically active esters and
`alcohols using esterase-catalyzed stereospecific transesterifications in organic media. J. Am.
`Chem. Soc. 106: 2687-2692.
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`Dropsy, E.P., Klibanov, A.M. 1984. Cholinesterase-catalyzed resolution of D,L-carnitine.
`Biotechnol. Bioen.q. 2._66: 911-915.
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`Cambou, B., Klibanov, A.M. 1984. Lipase-catalyzed production of optically active acids via
`asymmetric hydrolysis of esters: effect of the alcohol moiety. Appl. Biochem. Biotechnol. 9:
`255-260.
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`69.
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`Zaks, A., Klibanov, A.M. 1984. Enzymatic catalysis in organic media at 100°C. Science 224:
`1249-1251.
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`70.
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`75.
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`76.
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`77.
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`78.
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`80.
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`82.
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`83.
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`84.
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`85.
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`86.
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`Cambou, B., Klibanov, A.M. 1985. Comparison of different strategies for the lipase-catalyzed
`preparative resolution of racemic acids and alcohols: asymmetric hydrolysis, esterification, and
`transesterification. Biotechnol. Bioenq. 2._66: 1449-1454.
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`Scollar, M.P., Sigal, G., Klibanov, A.M. 1985. Preparative resolution of D,L-threonine catalyzed
`by immobilized phosphatase. Biotechnol. Bioen,q. 27: 247-252.
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`Ahern, T.J., Klibanov, A.M. 1985. The mechanism of irreversible enzyme inactivation at 100°C.
`Science 228: 1280-1284.
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`Cesti, P., Zaks, A., Klibanov, A.M. 1985. Preparative regioselective acylation of glycols by
`enzymatic transesterification in organic solvents. Appl. Biochem. Biotechnol. 1"1 401-407.
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`Zaks, A., Klibanov, A.M. 1985. Enzyme-catalyzed processes in organic solvents. Proc. Natl.
`Acad. Sci. USA 82: 3192-3196.
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`Kazandjian, R.Z., Klibanov, A.M. 1985. Regioselective oxidation of phenols catalyzed by
`polyphenol oxidase in chloroform. J. Am. Chem. Soc. 107: 5448-5450.
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`Kirchner, G., Scollar, M.P., Klibanov, A.M. 1985. Resolution of racemic mixtures via lipase
`catalysis in organic solvents. J. Am. Chem. Soc. 107: 7072-7076.
`
`Lavin, A., Sung, C., Klibanov, A.M., Langer, R.S. 1985. Enzymatic removal of bilirubin from the
`blood: a potential treatment of neonatal jaundice. Science 230: 543-545.
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`Hammond, D.A., Karel, M., Klibanov, A.M., Krukonis, V.J. 1985. Enzymatic reactions in
`supercritical gases. Appl. Biochem. Biotechnol. 1"1 393-400.
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`Sung, C., Lavin, A., Klibanov, A.M., Langer, R. 1986. An immobilized enzyme reactor for the
`treatment of severe jaundice. Trans. Am. Soc. Artif. Intern. Or.qans. 31 264-269.
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`Kazandjian, R.Z., Dordick, J.S., Klibanov, A.M. 1986. Enzymatic analyses in organic solvents.
`Biotechnol. Bioen.q. 2._88: 417-421.
`
`Klibanov, A.M. 1986. Enzymes that work in organic solvents. CHEMTECH 1._66: 354-359.
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`Dordick, J.S., Klibanov, A.M., Marietta, M.A. 1986. Horseradish peroxidase-catalyzed
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`Sung, C., Lavin, A., Klibanov, A.M., Langer, R. 1986. An immobilized enzyme reactor for the
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`Zaks, A., Klibanov, A.M. 1986. Substrate specificity of enzymes in organic solvents vs. water is
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`Dordick, J.S., Marietta, M.A., Klibanov, A.M. 1986. Peroxidases depolymerize lignin in organic
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`Zale, S.E., Klibanov, A.M. 1986. Why does ribonuclease irreversibly inactivate at high
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`87. Grunwald, J., Wirz, B., Scollar, M.P., Klibanov, A.M. 1986. Asymmetric oxidoreductions
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`88.
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`95.
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`Therisod, M., Klibanov, A.M. 1986. Facile enzymatic preparation of monoacylated sugars in
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`Boeriu, C.G., Dordick, J.S., Klibanov, A.M. 1986. Enzymatic reactions in liquid and solid
`paraffins: application for enzyme-based temperature abuse sensors. Bio/Technol. 4: 997-999.
`
`Klibanov, A.M., Cambou, B. 1987. Enzymatic production of optically active compounds in
`biphasic aqueous-organic systems. Meth. Enzymol. 136C: 117-137.
`
`Ahern, T.J., Casal, J.l., Petsko, G.A., Klibanov, A.M. 1987. Control of oligomeric enzyme
`thermostability by protein engineering. Proc. Natl. Acad. Sci. USA 84: 675-679.
`
`Shaw, J.F., Klibanov, A.M. 1987. Preparation of various glucose esters via lipase-catalyzed
`hydrolysis of glucose pentaacetate. Biotechnol. Bioenq. 2!9: 648-651.
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`Casal, J.l., Ahem, T.J., Davenport, R.C., Petsko, G.A., Klibanov, A.M. 1987. Subunit interface
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`
`Dordick, J.S., Marietta, M.A., Klibanov, A.M. 1987. Polymerization of phenols catalyzed by
`peroxidase in non-aqueous media. Biotechnol. Bioenq. 3_0: 31-36.
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`Visuri, K., Klibanov, A.M. 1987. Enzymatic production of high fructose corn syrup (HFCS)
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`96. Margolin, A.L., Crenne, J.-Y., Klibanov, A.M. 1987. Stereoselective oligomerizations catalyzed
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`97.
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`99.
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`Volkin, D.B., Klibanov, A.M. 1987. Thermal destruction processes in proteins involving cystine
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`Therisod, M., Klibanov, A.M. 1987. Regioselective acylation of secondary hydroxyl groups in
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`Ahern, T.J., Klibanov, A.M. 1987. Analysis of processes causing thermal inactivation of
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`100. Margolin, A.L., Klibanov, A.M. 1987. Peptide synthesis catalyzed by lipases in anhydrous
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`101.
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`Barzana, E., Klibanov, A.M., Karel, M. 1987. Enzyme-catalyzed gas phase reactions. Appl.
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`102. Margolin, A.L., Tai, D.-F., Klibanov, A.M. 1987. Incorporation of D-amino acids into peptides
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`103. Chopineau, J., McCafferty, F.D., Therisod, M., Klibanov, A.M. 1988. Production of
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`104. Mullon, C.J.P., Klibanov, A.M., Langer, R. 1988. Kinetics of soluble bilirubin oxidase and
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`105. Riva, S., Chopineau, J., Kieboom, A.P.G., Klibanov, A.M. 1988. Protease-catalyzed
`regioselective esterification of sugars and related compounds in anhydrous dimethylformamide.
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`106.
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`107.
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`108.
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`Tomazic, S.J., Klibanov, A.M. 1988. Mechanisms of irreversible thermal inactivation of Bacillus
`a-amylases. J. Biol. Chem. 263: 3086-3091.
`
`Tomazic, S.J., Klibanov, A.M. 1988. Why is one Bacillus a-amylase more resistant against
`irreversible thermoinactivation than another? J. Biol. Chem. 263: 3092-3096.
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`Zaks, A., Klibanov, A.M. 1988. Enzymatic catalysis in non-aqueous solvents. J. Biol. Chem.
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`109. Riva, S., Klibanov, A.M. 1988. Enzymo-chemical regioselective oxidation of steroids without
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`
`110.
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`Zaks, A., Klibanov, A.M. 1988. The effect of water on enzyme action in organic media. J. Biol.
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`111. Russell, A.J., Klibanov, A.M. 1988. Inhibitor-induced enzyme activation in organic solvents.
`J. Biol. Chem. 263: 11624-11626.
`
`112. Weaver, J.C., Williams, G.B., Klibanov, A.M., Demain, A.L. 1988. Gel microdroplets: rapid
`detection and enumeration of individual microorganisms by their metabolic activity.
`Bio/Technoloqy 6: 1084-1089.
`
`113. Kitaguchi, H., Tai, D.-F., Klibanov, A.M. 1988. Enzymatic formation of an isopeptide bond
`involving the E-amino group of lysine. Tetrahedron Lett. 2.~9: 5487-5488.
`
`114. Sakurai, T., Margolin, A.L., Russell, A.J., Klibanov, A.M. 1988. Control of enzyme enantio-
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`
`115. Volkin, D.B., Klibanov, A.M. 1989. Mechanism of thermoinactivation of immobilized glucose
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`
`116. Chinsky, N., Margolin, A.L., Klibanov, A.M. 1989. Chemoselective enzymatic monoacylation of
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`
`117. Russell, A.J., Trudel, L.J., Skipper, P.L., Groopman, J.D., Tannenbaum, S.R., Klibanov, A.M.
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`118.
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`Barzana, E., Karel, M., Klibanov, A.M. 1989. Enzymatic oxidation of ethanol in the gaseous
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`
`119. Kitaguchi, H., Fitzpatrick, P.A., Huber, J.E., Klibanov, A.M. 1989. Enzymatic resolution of
`racemic amines: crucial role of the solvent. J. Am. Chem. Soc. 111: 3094-3095.
`
`120. Dorval, B.L., Chow, M., Klibanov, A.M. 1989. Stabilization of poliovirus against heat
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`121.
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`Barzana, E., Klibanov, A.M., Karel, M. 1989. A colorimetric method for the enzymatic analysis
`of gases: the determination of ethanol and formaldehyde vapors using solid alcohol oxidase.
`Anal. Biochem. 182: 109-115.
`
`122. Volkin, D.B., Klibanov, A.M. 1989. Minimizing protein inactivation. In: Protein Function - A
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`124.
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`125.
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`Klibanov, A.M. 1989. Enzymatic catalysis in anhydrous organic solvents. Trends Biochem.
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`Kanerva, L.T., Klibanov, A.M. 1989. Hammett analysis of enzyme action in organic solvents.
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`
`Burke, P.A., Smith, S.O., Bachovchin, W.W., Klibanov, A.M. 1989. Demonstration of structural
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`126. Kitaguchi, H., Klibanov, A.M. 1989. Enzymatic peptide synthesis via segment condensation in
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`127.
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`Braco, L., Dabulis, K., Klibanov, A.M. 1990. Design of novel receptors by molecular imprinting
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`
`128. Margolin, A.L., Klibanov, A.M. 1990. Enzymatic synthesis of peptides in anhydrous organic
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`129. Dorval, B.L., Chow, M., Klibanov, A.M. 1990. Lysine and other diamines dramatically stabilize
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`130.
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`Klibanov, A.M. 1990. Asymmetric transformations catalyzed by enzymes in organic solvents.
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`131. Adams, K.A.H., Chung, S.-H., Klibanov, A.M. 1990. Kinetic isotope effect investigation of
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`132.
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`Liu, W.R., Langer, R., Klibanov, A.M. 1991. Moisture-induced aggregation of lyophilized
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`133. Rubio, E., Fernandez-Mayorales, A., Klibanov, A.M. 1991. Effect of the solvent on enzyme
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`134. Chang, N., Hen, S.J., Klibanov, A.M. 1991. Protein separation and purification in neat dimethyl
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`
`135. Volkin, D.B., Staubli, A., Langer, R., Klibanov, A.M. 1991. Enzyme thermoinactivation in
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`
`136. Schulze, B., Klibanov, A.M. 1991. Inactivation and stabilization of subtilisins in neat organic
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`137.
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`Fitzpatrick, P.A., Klibanov, A.M. 1991. How can the solvent affect enzyme enantioselectivity?
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`
`138. Margolin, A.L., Fitzpatrick, P.A., Dubin, P.L., Klibanov, A.M. 1991. Chemo-enzymatic synthesis
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`
`139. Sluzky, V., Tamada, J.A., Klibanov, A.M., Langer, R. 1991. Kinetics of insulin aggregation in
`aqueous solutions upon agitation in the presence of hydrophobic surfaces. Proc. Natl. Acad.
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`140. Gardossi, L., Bianchi, D., Klibanov, A.M. 1991. Selective acylation of peptides catalyzed by
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`
`141. Kitaguchi, H., Ono, M., Itoh, I., Klibanov, A.M. 1991. Regioselective modification of lysine’s
`amino groups by proteases. A.qric. Biol. Chem. 5,5: 3067-3073.
`
`142. Dabulis, K., Klibanov, A.M. 1992. Molecular imprinting of proteins and other macromolecules
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`143. Chang, N., Klibanov, A.M. 1992. Chromatographic separation of proteins in neat organic
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`144.
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`145.
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`Tawaki, S., Klibanov, A.M. 1992. Inversion of enzyme enantioselectivity mediated by the
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`
`Fitzpatrick, P.A., Ringe, D., Klibanov, A.M. 1992. Computer-assisted modeling of subtilisin
`enantioselectivity in organic solvents. Biotechnol. Bioenq. 4_0: 735-742.
`
`146. Sluzky, V., Klibanov, A.M., Langer, R. 1992. Mechanism of insulin aggregation and
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`147.
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`Burke, P.A., Griffin, R.G., Klibanov, A.M. 1992. Solid-state NMR assessment of enzyme active
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`148. Klyachko, N.L., Klibanov, A.M. 1992. Oxidation of dibenzothiophene catalyzed by hemoglobin
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`149. Narayan, V.S., Klibanov, A.M. 1993. Are water-immiscibility and apolarity of the solvent
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`150. Dabulis, K., Klibanov, A.M. 1993. Dramatic enhancement of enzymatic activity in organic
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`151.
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`Terradas, F.,