Curriculum Vitae
`
`Personal Details
`
`
`David Peter Joseph Hornby
`
`9th October 1958
`
`Married with two children
`
`British
`
`Department of Molecular Biology and
`Biotechnology
`The University of Sheffield
`Firth Court
`Western Bank
`Sheffield
`S10 2TN
`
`
`+44(0)114 222 4232
`
`dphornby@gmail.com
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`d.hornby@shef.ac.uk
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`
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` 9, Lessingham Road
`Widnes
`Cheshire
`WA8 9FU
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`+44(0)151 424 8931
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`+44(0)7887 622 153
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`http://www.sheffield.ac.uk/mbb/index
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`http://www.krebs.group.shef.ac.uk/
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`http://lifesciencesutc.co.uk/
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`http://utcinnovationlabs.blogspot.co.uk/
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`http://undergradtutorialblog.blogspot.co.uk/
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`https://molecules2market.blogspot.com
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`http://widnesscibar.blogspot.co.uk/
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`http://qcqcclub.blogspot.co.uk/
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`http://lutcbtec.blogspot.co.uk/
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`http://nlanotebook.blogspot.co.uk/
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`http://oneminutemicroblog.blogspot.co.uk/
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`Twitter
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`1
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` @dvdhrnby
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`Name
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`Date of birth
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`Marital status
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`Nationality
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`Work Address
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`Telephone (direct)
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`Email (personal)
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`Email (academic 1)
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`Address (home)
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`Telephone (home)
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`Mobile
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`Departmental web site
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`Krebs Institute web site
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`Life Sciences UTC web site
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`Blog Sites
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`MTX1003
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`2015
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`2013-present
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`2013-2015
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`2012-2013
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`2007- 2012
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`2008-2011
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`1999 - 2007
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`2011 - present
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`2004 – present
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`2002 – 2008
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`1999 - 2002
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`1998 - 1999
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`1997 - 1999
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`1994 - 1998
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`1993 - present
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`1992-1993
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`1990-1994
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`1990
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`1986 - 1990
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`Employment Record
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`Elected Fellow of the Royal Society of Biology
`
`Professor of Biochemistry
`Department of Molecular Biology and Biotechnology
`(University of Sheffield)
`
`Director of Research and Innovation
`Liverpool Life Science UTC (60% and 40% as above)
`
`Sabbatical visitor Liverpool School of Tropical Medicine
`
`Head of the Department of Molecular Biology and
`Biotechnology
`
`Director of Enterprise in Life Sciences
`
`Professor of Biochemistry
`Department of Molecular Biology and Biotechnology
`
`Acting Chair Bioserv UK Ltd.
`
`Director of Combigen Ltd
`
`Director of Protealysis Ltd
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`Director of the Transgenomic Research Laboratory
`
`Reader
`Department of Molecular Biology and Biotechnology
`
`Director of Invector Ltd
`
`Senior Lecturer
`Department of Molecular Biology and Biotechnology
`
`Visiting Scientist
`Department Molecular Biology
`The Scripps Research Institute
`California
`
`Study leave (Host Professor P. Wright)
` Department of Molecular Biology
` The Scripps Research Institute
` California
`
`Lecturer
`Department of Molecular Biology and Biotechnology
`
`EMBO fellowship (Host Dr. I. Mattaj)
`Gene Expression Program
`EMBL
`
`Temporary lecturer
` Department of Biochemistry
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`2
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`

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`
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`1985-1986
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`1984
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`1980-84
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`1977-80
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`Postdoctoral research associate (SNF)
`Biozentrum der Universitat Basel,
`Switzerland Supervisor Professor T.A. Bickle
`
`Postdoctoral research associate (SERC)
`Departments of Biochemistry, Sheffield (Dr. P.C.Engel)
`and Genetics, Leeds (Drs. J. Wootton and M. McPherson)
`
`PhD (Biochemistry)
`
`BSc Hons (Biochemistry)
`University of Sheffield
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`Publications
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`1. Hornby, D.P., Engel, P.C. & Hatanaka, S.-I. (1983). Beef liver glutamate dehydrogenase: A study of the
`oxidative deamination of alternative amino acid substrates retaining the correct spacing of the two carboxylate
`groups. Int. J. Biochem. 15, 495-500.
`2. Hornby, D.P. & Engel, P.C. (1984). Characterisation of Peptostreptococcus asaccharolyticus glutamate
`dehydrogenase purified by dye-ligand chromatography. J. Gen. Micro. 130, 2385-2394.
`3. Hornby, D.P. & Engel, P.C. (1984). A kinetic study of the oxidative deamination of L-glutamate by
`Peptostreptococcus asaccharolyticus glutamate dehydrogenase using a variety of coenzymes. Eur. J.
`Biochem., 143, 557-560.
`4. Hornby, D.P., Aitchison, M.J. & Engel, P.C. (1984). The kinetic mechanism of Ox-liver glutamate
`dehydrogenase in the presence of the allosteric effector ADP. Biochem. J., 223, 161-168.
`5. Rice, D.W., Hornby, D.P. & Engel, P.C. (1985). Crystallization of an NAD-dependent glutamate dehydrogenase
`from Clostridium symbiosum. J. Mol. Biol., 181, 147-149.
`6. Hornby, D.P., Engel, P.C. & Hatanaka, S.-I. (1985). Kinetic studies of Ox-liver glutamate dehydrogenase:
`Oxidative deamination of two glutamate analogues, L-threo--methylglutamate and L-amino--
`nitraminobutyrate in the presence of the allosteric effector ADP. Int. J. Biochem., 17, 851-854
`7. Rice, D.W., Baker, P.J., Farrants, G.W. & Hornby, D.P. (1986) The crystal structure of glutamate
`dehydrogenase from Clostridium symbiosum at 0.6nm resolution. Biochem. J., 242, 789-795.
`8. Hornby, D.P., Muller, M. & Bickle, T.A. (1986). High-level expression of the EcoP1 modification methylase
`gene and characterization of the gene product. Gene, 54, 239-245.
`9. Sharrocks, A. & Hornby, D.P. (1988). A rapid method for BAL31 deletion analysis. Nucl. Acids Res., 15, 8564.
`10. Humblin, M., Suri, B., Rao, D., Hornby, D.P., Eberle, H., Pripfl, T., Kennel, S. & Bickle, T.A. (1988) Nucleotide
`sequence of the EcoP1 restriction and modification operon and of the EcoP15 mod gene. J. Mol Biol., 200, 23-
`29.
`11. Sharrocks A.D. & Hornby D.P. (1991) Transcriptional analysis of the phage P1 restriction and modification
`genes. Mol. Microbiol., 5, 685-694.
`12. Sharrocks, A.D. & Hornby, D.P. (1991) The production of single-stranded probes for S1 mapping.
`Biotechniques, 10, 426-428.
`13. Dalton, A., Langston, S., Blackburn, G.M. & Hornby, D.P. (1992) Characterization and purification of a novel
`dATP binding protein in eukaryotes. Biochem. J., 287, 871-879.
`14. Kusiak, M., Price, C., Rice, D.W. & Hornby, D.P. (1992) The HsdS polypeptide of the Type I restriction enzyme
`EcoR124 is a sequence specific DNA binding protein. Mol. Microbiol., 12, 461-466.
`15. Taylor, C., Ford, K., Connolly, B.A., Hornby, D.P. (1993) Determination of the order of substrate addition to
`MspI DNA methyltransferase using a novel mechanism based inhibitor Biochem. J., 291, 498-504
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`16. Ford, K., Taylor, C., Connolly, B.A. & Hornby, D.P. (1993) The interaction of MspI DNA methyltransferase with
`S-adenosyl-L-methionine and DNA. J. Mol. Biol., 230, 779-786.
`17. Baldwin, G.S., Kelly, S., Connolly, B.A., Price, N.C. & Hornby, D.P. (1993) Ligand-induced conformational
`states of the cytosine-specific DNA methyltransferase M.HgaI-2. J. Mol. Biol., 235, 543-553.
`18. Ford, K., Waltho, J. & Hornby, D.P. (1994).Component analysis of the mammalian deoxy NTPase. Biochem.
`J., 298, 727-732.
`19. Syed, S. E.-H., Hornby, D.P. & Engel, P.C. (1993) Relative susceptibility of two cysteine residues in clostridial
`glutamate dehydrogenase to modification by thiol-specific reagents and the use of protection studies to
`measure coenzyme binding. Biochem. J., 298, 107-113.
`20. Blackburn, G.M., Hornby, D.P., Mekhalfia, A. & Shore, P. (1993) Synthesis and biological evaluation of (S)-
`Azaadomet, a stable isosteric acid isoelectronic analogue of AdoMet, S-adenosyl-L-methionine
`Col.Czech.Chem.Comun., 58, 116-119
`21. Soultanas, P., Andrews, P.D., Burton, D.R. & Hornby, D.P. (1993). Modulation of human DNA
`methyltransferase activity and messenger-RNA levels in the monoblast cell-line U937 induced to differentiate
`with dibutyryl-cyclic-amp and phorbol ester. Mol. Endocrinol., 11, 191-200.
`22. Hornby, D.P., Whitmarsh, A. Pinarbasi, H. Kelly, S.M., Price, N.C., Shore, P., Baldwin,G.S, Waltho, J. (1994).
`The DNA recognition subunit of a DNA methyltransferase is predominantly a molten globule in the absence of
`DNA. FEBS Lett., 355, 57-60.
`23. Ford, K.G., Alharrasy, W.S.& Hornby, D.P. (1995) Identification of direct-repeat-binding protein-1 (DRP-1), a
`DNA-binding protein that binds specifically to the malic enzyme gene promoter direct repeat element. Biochem.
`J., 311, 901-904
`24. Pinarbasi, E. Elliott, J. & Hornby, D.P. (1996) Activation of a yeast pseudo DNA methyltransferase by deletion
`of a single amino-acid. J. Mol. Biol., 257, 804-813
`
`25. Henderson, R.M., Schneider, S. Li, Q.A. Hornby, D.P., White, S.J., Oberleithner, H. (1996). Imaging ROMK1
`inwardly rectifying ATP-sensitive K+ channel protein using atomic-force microscopy. Proc.Nat. Acad.Sci. USA,
`93, 8756-8760.
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`26. Brewis, I.A., Clayton, R., Barratt, C.L., Hornby, D.P. & Moore H.D. (1996) Recombinant human zona pellucida
`glycoprotein 3 induces calcium influx and acrosome reaction in human spermatozoa. Mol. Hum. Reprod., 2,
`583-589
`27. Austin, N., Li, Q., Hornby, D.P., & White, S.J. (1996). Renal and extrarenal localisation of the RACTK1 K+ channel
`by immunofluorescence. J. Physiol., 497, 94.
`28. Austin, N., Li, Q.., Hornby, D.P. & White, S..J. (1996). Renal and extra renal localisation of ROMK and RACTK
`proteins by Immunocytochemistry J. American Soc. Nephrol., 7 A0139
`29. Chapman, N.R., Kessopoulou, E., Hornby, D.P., Cooke, I.D. & Barratt, C.L. (1997) The polypeptide backbone
`of recombinant human ZP3 induces acrosomal exocytosis in human spermatozoa. Biochem. J., 330, 839-845.
`30. Whitmarsh, A.J., Woolnough, M.J., Moore, H.D., Hornby, D.P. & Barratt, C.L. (1996) Biological activity of
`recombinant human ZP3 produced in vitro: potential for a sperm function test. Mol. Hum. Reprod., 2, 911-919
`31. Beesley, A.H., Hornby, D. & White, S.J. (1998) Regulation of distal nephron K+ channels (ROMK) mRNA
`expression by aldosterone in rat kidney. J. Physiol. (Lond) 509, 629-34
`32. Pinarbasi, E., Kan, M.S., Duran, C., Ford, G.C. & Hornby, D.P. (1998) Substitution of the conserved
`phenylalanine in the S-adenosyl-L-methionine binding site of M.MspI with tyrosine modifies the kinetic
`properties of the enzyme. Biol. Chem. 379, 591-594
`33. Hornby, D.P. & Ford, G.C. (1998) Protein mediated base flipping. Curr.Opinion Biotech. 9, 354-358
`34. Brewis, I.A., Clayton, R., Browes, C.E., Martin, M., Barratt, C.L., Hornby, D.P. & Moore, H.D. (1998) Tyrosine
`phosphorylation of a 95 kDa protein and induction of the acrosome reaction in human spermatozoa by
`recombinant human zona pellucida glycoprotein 3. Mol. Hum. Reprod., 4, 1136-44
`35. Hurd, P.J., Whitmarsh, A.J., Baldwin,G.S., Kelly,S.M., Waltho, J.P., Price, N.C., Connolly, B.A. & Hornby, D.P.
`(1999) Mechanism-based inhibition of C5-cytosine DNA methyltransferases by 2-H-pyrimidinone. J.Mol.Biol.
`286, 389-401
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`36. Harris, V.H.., Hamilton, A., Williams, D.M., & Hornby, D.P. (1999) Studying the function of methyltransferases
`using nucleotide analogue based random mutagenesis. Collection Symp.Series 2 209-212
`37. Thompson, M.J., Mekhalfia, A., Hornby,D.P. & Blackburn, G.M. (1999) Synthesis of two stable nitrogen
`analogues of S-adenosyl-L-methionine. J.Org. Chem. 64 7467-7473
`38. Matin, M. & Hornby, D.P. (2000) A positive selection vector combining tetracycline resistance that eliminates
`the need for bacterial plating. Anal. Biochem. 278, 46-51
`39. Ortega, B., Beesley, AH., Hornby, DP., White SJ. (2000) Tracing the Origin of the RACTK1 K K+ Channel.
`Biochem. Biophys.Res.Comm. 277, 147-151
`40. Dickman M. & Hornby, D.P. (2000) Isolation of Single-Stranded DNA Using Denaturing DNA Chromatography.
`Anal.Biochem. 284, 164-167.
`41. Rosler, A., Panayotou, G., Hornby, D.P., Barlow, T., Brown, T., Pearl, L.H., Savva,. R. & Blackburn, G.M.
`(2000) The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues.
`Nucleosides Nucleotides Nucleic Acids. 19, 1505-16
`42. Lee, S.E., Sidorov, A., Gourlain, T., Mignet, N., Thorpe, SJ., Brazier, J.A., Dickman, M.J., Hornby, D.P.,
`Grasby, J.A. & Williams, D.M. (2001) Enhancing the Catalytic Repertoire of Nucleic Acids: A Systematic Study
`of Linker Length and Rigidity. Nucleic Acid Res 29, 1565-1573.
`43. Pinarbasi H., Pinarbasis E. Hornby D.P. (2001) Substitution of the Conserved Cysteine with Glycine
`(Cys82Gly) of Agmenellum quadruplicatum Methylase AquI (M.Aqu1) is not Cytotoxic to E.coli. Turk. J. Biol. 25
`177-184
`44. Dickman, M.J., Matin, M.M. & Hornby, D.P. (2002) High-Throughput Analysis of Nucleic Acid Modification
`Reactions Using Ion-Pair Reverse-Phase High-Performance Liquid Chromatography. Anal. Biochem., 301 290-
`297
`45. Dickman, M.J., Conroy, M.J., Grasby, J.A. & Hornby, D.P. (2002) RNA Footprinting Analysis using ion pair
`reverse phase Liquid Chromatography. RNA, 8 247-251
`46. Matin, M.M., Andrews PW. & Hornby, D.P. (2002) Multi-dimensional differential display via ion-pair reversed-
`phase denaturing high performance liquid chromatography. Anal. Biochem., 304 47-54
`47. Matin, M.M., Baumer, A. & Hornby, D.P. (2002) An Analytical Method for the Detection of Methylation
`Differences at Specific Chromosomal Loci Using Primer Extension and Ion Pair Reverse Phase HPLC. Human
`Mutation, 20 305-311
`48. Bahrami, A., Dickman, M.J., Matin, M.M., Ashby, J., Brown, P.E., Conroy, M., Fowler, G.J.S., Rose, J.P., Sheikh,
`Q.I., Yeung, A. & Hornby, D.P. (2002) The use of fluorescent DNA-intercalating dyes in the analysis of DNA via
`ion-pair reversed-phase denaturing high performance liquid chromatography (IP RP DHPLC). Anal. Biochem.,
`309 248-252
`49. Dickman, M.J., Ingleston, S.M., Sedelnikova, S.E., Rafferty, J. B., Lloyd, R.G., Grasby, J.A. & Hornby, D.P.
`(2002) The RuvABC Resolvasome: Quantitative Analysis of RuvA and RuvC Assembly on Junction DNA. Eur.
`J. Biochem. 269 5492-5501
`50. Zhou, L., Cheng, X., Connolly, B.A., Dickman, M.J., Hurd, P.J. & Hornby, D.P. (2002) Zebularine: a novel DNA
`methylation inhibitor that forms a covalent complex with DNA Methyltransferases. J. Mol. Biol. 321 591-599
`51. Pinarbasi, H., Pinarbasi, E. & Hornby, D.P. (2002) Recombinant alpha and beta subunits of M.AquI constitute
`an active DNA methyltransferase. J. Biochem. Mol. Biol. 35 348-51.
`52. Ingleston, S.M., Dickman, M.J., Grasby, J.A., Hornby, D.P., Sharples, G.J., & Lloyd R.G. (2002) Holliday
`junction binding and processing by the RuvA protein of Mycoplasma pneumoniae. Eur J Biochem. 269 1525-
`33.
`53. Pinarbasi, H., Pinarbasi, E. & Hornby, D.P. (2003) The Small Subunit of M. AquI Is Responsible for Sequence-
`Specific DNA Recognition and Binding in the Absence of the Catalytic Domain. J. Bacteriol. 185 1284-8.
`54. Roberts, R.J., Belfort, M., Bestor, T, Bhagwat,, A.S., Bickle, T.A., Bitinaite, J., Blumenthal, R.M., Degtyarev,
`S.Kh., Dryden, D.T.F., Dybvig, K., Firman, K., Gromova, E.S., Gumport, R.I., Halford, S.E., Hattman, S.,
`Heitman, J., Hornby, D.P., Janulaitis, A., Jeltsch, A., Josephsen, J., Kiss, A., Klaenhammer, T.R., Kobayashi, I.
`Kong, H., Krüger, D., Lacks, S., Marinus, M.G., Miyahara, M., Morgan, R.D., Murray, N.E., Nagaraja, V.,
`Piekarowicz, A., Pingoud, A. , Raleigh, E., Rao, D.N., Reich, N., Repin, V. E., Selker, E.U., Shaw, P.C.,.Stein,
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`D.C., Stoddard, B.L., Szybalski, W., Trautner, T.A., Van Etten, J.L., Vitor, J.M.B., Wilson, G.G. & Xu, S.Y.
`(2003) A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their
`genes .Nucl. Acids Res. 31, 1805-1812
`55. Harris, V.H., Smith, C.L., Cummins, W.J., Hamilton, A.L., Hornby, D.P. & Williams, D/M. (2003) Recognition of
`base pairing by DNA polymerases during nucleotide incorporation: the properties of the mutagenic nucleotide
`dPTP. Org. Biomo.l Chem. 21; 2070-4.
`56. Harris, V.H., Smith C.L. Cummins, W.J., Hamilton, A.L., Adams, H., Dickman M., Hornby, D.P. & Williams D.M.
`(2003). The effect of Tautomeric Constant on the Specificity of Nucleotide Incorporation during DNA
`Replication: Support for the Rare Tautomer Hypothesis of Substitution Mutagenesis. J.Mol.Biol. 326, 1389-
`1401.
`57. Dickman, M.J., Sedelnikova S.E., Rafferty, J.B. & Hornby, D.P. (2004) Rapid analysis of protein nucleic acid
`complexes using MALDI TOF mass spectrometry and ion pair reverse phase liquid chromatography. J.
`Biochem.Biophys.Methods 58 39-48
`58. Dickman MJ, Hornby D.P. (2006) Enrichment and analysis of RNA centred on ion pair reverse phase
`methodology. RNA. 12, 691-6.
`59. Hanna C, Gjerde D, Nguyen L, Dickman M, Brown P, Hornby D.P. (2006) Micro-scale open-tube capillary
`separations of functional proteins. .Anal. Biochem. 350, 128-37.
`
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`60. Waghmare SP, Pousinis P, Hornby DP, Dickman MJ. (2009) Studying the mechanism of RNA separations
`using RNA chromatography and its application in the analysis of ribosomal RNA and RNA:RNA interactions.
`Journal of Chromatography, 1216, 1377-1382.
`
`
`61. Xu W, Liu L, Brown NJ, Christian S, Hornby D. (2012) Quantum dot- conjugated anti-GRP78 scFv inhibits
`cancer growth in mice. Molecules 17, 796-808.
`
`62. Qureshi MS, Sheikh QI ,Hill R, Brown PE ,Dickman MJ, Tzokov SB, Rice DW, Gjerde DT, Hornby DP.
`(2013) Affinity filtration coupled with capillary-based affinity purification for the isolation of protein
`complexes. Anal Biochem. 439 47-49.
`
`
`63. Xu W, Liu L & Hornby D. (2012). c-IAP1 binds and processes PCSK9 protein: linking the c-IAP1 in a TNF-
`α pathway to PCSK9-mediated LDLR degradation pathway. Molecules 17 12086-101.
`
`64. Snijders AP, Hautbergue GM, Bloom A, Williamson JC, Minshull TC, Phillips HL, Mihaylov SR, Gjerde
`DT, Hornby DP, Wilson SA, Hurd PJ, Dickman MJ (2015) Arginine methylation and citrullination of splicing
`factor proline- and glutamine-rich (SFPQ/PSF) regulates its association with mRNA. RNA. 21 347-59.
`
`65. Hornby DP et al (100 school students) Royal Society Special Edition. A new model organism for teaching
`omics in schools https://issuu.com/ysjournal/docs/issue_17
`
`
`
`
`Book chapters
`1. Hornby, D.P. (1987) Application of biological specificity in the purification of enzymes in EEC Handbook of
`Biomedical Research Techniques
`2. Hornby, D.P. (1991) DNA Methyltransferases. Meth. Mol. Biol., 12 1-16.
`3. Hornby, D.P., Ford, K. & Shore, P. (1991). Purification of DNA binding proteins. Techniques in Protein
`Purification., 11, 1-13
`4. Whitmarsh, A., Ford, K. & Hornby, D.P. (1993). Expression of DNA binding proteins in E.coli. Meth. Mol. Biol.,
`11, 273-285.
`5. Whitmarsh, A., Smith, J. & Hornby, D.P. (1994) Protein fusions as an aid to purification. in Highly selective
`separations in biotechnology. pp 204-229 Chapman & Hall
`6. Hurd, P.J. & Hornby, D.P. (1996) Expression systems and fusion proteins in Proteins Labfax, Ed. N.C. Price,
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`Academic Press.
`7. Hornby DP. DNA Methylation (2001) Encyclopedia of Life Sciences, Macmillan
`8. Gjerde, D.T., Hanna, C.P., Bonn, G. and Hornby, D.P. DNA Chromatography (2003) Encyclopaedia of
`Molecular Biology, Wiley.
`9. Hornby, D.P. & Sheikh, Q-I (2013) in Tools and Techniques in Biomolecular Science, Edited by Aysha Divan
`and Janice Royds Chapter 1 Gene Cloning Essentials Oxford University Press.
`Published Books
`Gjerde, D.T., Hanna, C.P. and Hornby, D.P.
`Gjerde, D.T., Hoang, L. and Hornby, D.P.
`
`Full Patent Applications
`
`DNA Chromatography (2002) Wiley
`RNA Chromatography (2009) Wiley.
`
`
`Method and systems for RNA analysis by matched ion polynucleotide
`chromatography (2000) USA
`
`Chromatographic method for RNA stabilization (2001) USA
`
`Apparatus and Method for separating and purifying polynucleotides
`(2001) USA
`
`Liquid Chromatographic Separation of Polynucleotides (2000) USA
`
`Methods and Reagents for Polynucleotide analysis (2000) USA
`
`Methods and Reagents for determination of Telomerase activity (2000)
`USA
`
`Methods and Reagents for Analysis of RNA structure and function
`(2000) USA
`
`Method for DNA foot-printing (2000) USA
`
`Method for detection of truncated proteins (2000) USA
`
`Method and system for the Preparation of cDNA (2001) USA
`
`Method for isolating single-stranded DNA (2001) USA
`
`Positive selection vectors (Invector) (2000) USA and UK
`
`Purification of recombinant proteins (2008) UK
`
`Media Activity relating to schools outreach for example
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`http://www.thesundaytimes.co.uk/sto/business/Industry/article1381878.
`ece
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`Year
`
`1983
`
`1985
`
`1985
`
`1986
`
`1988
`
`1988
`
`1989
`
`1989
`
`1990
`
`1990
`
`1990
`
`1990
`
`1990
`
`1990
`
`1990
`
`1991
`
`1991
`
`1991
`
`
`
`
`
`Lectures
`
`Venue
`
`Title
`
`Northern University Enzyme Group Meeting ,
`University of Sheffield
`
`Kinetic properties of a Bacterial
`Glutamate Dehydrogenase
`
`University of Regensburg, Germany
`
`Structure and Function of Glutamate
`Dehydrogenase
`
`Biozentrum, Department of Structural Biology,
`University of Basel, Switzerland
`
`Structure of a Bacterial Glutamate
`Dehydrogenase
`
`Biozentrum, University of Basel, Switzerland,
`Annual Research Symposium
`
`Nucleotide Sequence of the Phage P1
`Modification Gene
`
`Cystic Fibrosis Trust, Annual Meeting, University
`of Manchester
`
`Plasmid Profiling in Pseudomonas
`cepacia
`
`Department of Biology, University of York
`
`SERC Molecular Recognition Group (Young
`British and American Investigators, University of
`Leicester)
`
`DNA Methylation in Bacterial and Man
`
`
`DNA Recognition and Restriction
`Enzymes
`
`University of Sheffield (NACON II)
`
`Complex Restriction Enzymes
`
`School of Biology, University of Manchester
`
`MRC Molecular Embryology Unit, London
`
`University of Southampton
`
`Genetics and Enzymology of DNA
`Methylation
`
`Genetics and Enzymology of DNA
`Methylation
`
`Genetics and Enzymology of DNA
`Methylation
`
`Second New England Biolabs Workshop on
`Biological DNA restriction and modification, Berlin
`
`Genetic Fusion between Mspl and the
`Human DNA Methyltransferases
`
`EMBL, Heidelberg
`
`EMBL, Heidelberg
`
`Society for Experimental Biology Meeting,
`University of Warwick
`
`Institute of Molecular and Cellular Biology,
`University of Edinburgh
`
`Bacterial DNA methyltransferases
`
`Mammalian DNA Methyltransferases
`
`DNA Methylation in Man
`
`Enzymology of DNA Methylation
`
`Biophysics Laboratory, Portsmouth Polytechnic
`
`Enzymology of DNA Methylation
`
`Department of Biochemistry, University of Bristol
`
`Protein Kinase C structure and
`function
`
`
`8
`
`

`

`1992
`
`1993
`
`1993
`
`1994
`
`1994
`
`1994
`
`1994
`
`1994
`
`1994
`
`1996
`
`1997
`
`1997
`
`1997
`
`1999
`
`1999
`
`1999
`
`1999
`
`1999
`
`2000
`
`
`
`
`
`
`Department of Molecular Biology, The Scripps
`Research Institute, USA
`
`
`Enzymology of DNA Methylation
`
`FASEB Meeting, DNA Modification, Vermont,
`USA
`
`Dynamic aspects of DNA
`methyltransferases
`
`Max-Planck Institute for Molecular Genetics,
`Berlin
`
`Activating a ‘latent’ DNA
`methyltransferase by site-directed
`mutagenesis
`
`University of Glasgow
`
`University of Newcastle
`
`Dynamic aspects of DNA
`methyltransferase
`
`Dynamic aspects of DNA
`methyltransferase
`
`Fourth New England Biolabs Restriction and
`Modification workshop, Vilnius, Lithuania
`
`Dynamic aspects of DNA
`methyltransferase
`
`Biochemical Society Meeting, University of Cardiff Dynamic aspects of DNA
`methyltransferase
`
`Max-Planck Institute for Molecular Genetics,
`Berlin
`
`Dynamic aspects of DNA
`methyltransferase
`
`Max-Planck Institute for Molecular Genetics,
`Berlin
`
`Multi-specific DNA Methyltransferase
`
`German Biochemical Society Meeting, University
`of Giessen
`
`Mechanistic aspects of cytosine-
`specific DNA methyltransferases
`
`Fifth, New England Biolabs Restriction and
`Modification Workshop, Igls (Austria)
`
`Site specific deldtion induced by
`methyltransferase mutants
`
`Department of Biochemistry, University of
`Leicester
`
`
`Site specific deletion induced by an
`engineered DNA methyltransferase
`
`Department of Biochemistry, University of
`Newcastle
`
`Site specific deletion induced by an
`engineered DNA methyltransferase
`
`University of Sheffield (NACON III)
`
`Site specific deletion induced by an
`engineered DNA methyltransferase
`
`Department of Applied Biology, University of Hull Mutagenesis and mutation detection
`
`Nycomed, Amersham
`
`IBC Conference, Seattle
`
`National Cancer Institute, Maryland, USA
`
`Functional Genomics
`
`Functional Genomics via High
`Throughput DHPLC
`
`Novel approaches to nucleic acid
`purification and quantification
`
`Department of Chemistry Chemical Biology
`Symposium, University of Sheffield
`
`Nucleic acid enzymology on the
`genomic scale
`
`9
`
`

`

`
`2000
`
`2000
`
`2001
`
`2001
`
`2001
`
`2002
`
`2002
`
`2002
`
`2002
`
`2002
`
`2007
`
`2007
`
`2007
`
`2007
`
`2008
`
`2009
`
`2009
`
`2010
`
`
`
`
`Symposium “Biological significance of DNA-
`methylation” Max Planck Institute for Genetics,
`Berlin
`
`
`Multi-functional DNA methyltransferase
`
`Inaugural meeting of the North of England
`Structural Biology Centre, University of Leeds
`
`Functional Genomics of the eukaryotic
`nucleus
`
`Physiology Society Meeting, University of York
`
`Mutational Analysis by DHPLC
`
`Reproductive Biology Society, University of
`Birmingham
`
`University of Sheffield (NACON IV)
`
`Department of Chemistry University of York
`
`Institute of Analytical Chemistry and
`Radiochemistry, University of Innsbruck, Austria
`
`Mutational Analysis by DHPLC
`
`Mutational Analysis of DNA
`methyltransferases
`
`The role of DNA methyltransferases in
`genome stability
`
`Post genomic Molecular Biology
`
`Institute of Analytical Chemistry and
`Radiochemistry, University of Innsbruck, Austria
`
`Experimental proteomics
`
`School of Biology, University of Portsmouth
`
`Department of Biochemistry, UMIST
`
`Institute of Cancer Research (London)
`
`SPORT BBSRC symposium University of
`Sheffield
`
`Nucleic acid enzymology and genomic
`integrity
`
`Nucleic acid enzymology and genomic
`integrity
`
`New approaches for the isolation of
`TAP tagged protein complexes
`
`Are 50 year old resin based
`technologies good enough for the
`demands of contemporary
`biochemistry?
`
`FEBS conference RNA and DNA modification
`(Aussois, France)
`
`A Novel technique for the isolation of
`protein complexes
`
`Department of Molecular Biology, University of
`Kent
`
`A Novel technique for the isolation of
`protein complexes
`
`Knowledge Transfer Event, University of Madrid
`
`New England Biolabs, Boston, USA
`
`Measuring the success of University
`Commercialisation
`
`Error prone mutagenesis and its
`application in analysis of base flipping
`proteins
`
`Lecture tour Libya n Universities and Research
`Institutes
`
`Contemporary Molecular Biology
`Teaching and Research
`
`New England Biolabs European Conference,
`Bremen, Germany. Restriction and Modification
`10
`
`Meeting summing up
`
`

`

`NACON University of Sheffield
`
`Meeting summing up
`
`Lecture tour Libya n Universities and Research
`Institutes
`
`Contemporary Molecular Biology
`Teaching and Research
`
`
`
`
`
`150th Anniversary Symposium Sir Frederick
`Gowland Hopkins, Cambridge University
`
`Sir Hans Krebs in Sheffield
`
`
`2010
`
`2010
`
`
`
`2011
`
`2011
`
`2012
`
`2012
`
`2012
`
`University of Portsmouth
`
`University of Bristol
`
`University Manchester (TSB symposium)
`
`Bioline/Meridian (London)
`
`2013
`
`2014
`
`2015
`
`
`Liverpool School of Tropical Medicine
`
`Manchester SEB Meeting 2014
`
`
`
`University of Portsmouth
`
`Inducing chromosomal condensation
`with a mutant DNA Methyltransferase
`
`Embedding Enterprise in
`Undergraduate Biochemistry :
`“Molecules to Market at Sheffield”
`
`The application of M13 phage in
`synthetic biology
`
`Synthetic Biology: opportunities for
`reagent development
`
`Error prone mutagenesis the origins of
`chromothripsis in prokaryotes
`The application of error prone
`polymerases and cloning vectors for
`synthetic biology
`The application of error prone
`polymerases for exploring minimal
`requirements for catalysis in a range of
`enzymes
`
`
`Professional Taught Courses
`
`Centre for Professional Advancement (CfPA) Co-ordinator and demonstrator on the 4-day lecture course on Protein
`Science (General introduction to proteins, protein structure, recombinant DNA technology, protein expression, mass
`spectrometry, chromatography and electrophoresis) Amsterdam and New Jersey (1997-2002)
`
`
`
`
`
`
`
`11
`
`

`

`Award
`
`
`Royal Society Schools
`Fund (2014-2016)
`
`MRC 2005-2010
`
`Research Funding
`
`Project
`
`
`
`The Mealworm: a school friendly
`model organism
`
`
`
`Nitric oxide co-ordinates early defence
`responses through mitochondrial
`regulation
`
`Value
`
`
`£10 000
`
`
`£371, 244 (residual amount after
`Prof I Charles left the University
`from over £1m)
`
`PMF2 (2008-2010)
`
`Protein complex technology
`
`BBSRC (2008-2012)
`
`Doctoral Training Grant (PhD)
`
`BBSRC (2005-2008)
`(joint with David Rice)
`
`EPSRC (2004-2008)
`(joint awardee)
`
`SRIF2 (2004-2007)
`
`EPSRC (2003-2005)
`(joint awardee)
`
`PhyNexus (2002)
`
`Varian Inc (2002)
`
`BBSRC Structural Proteomics of
`Rational Targets (SPORT)
`
`
`Gel free Proteomics: High Throughput
`Microfluidic Shotgun Analysis for
`Organisms…
`
`Protein & Nucleic Acid Chemistry
`Facilities
`
`International Centre for Advanced
`Research in Identification Science
`
`Gel-free proteomics pilot study
`
`Nucleic acid analysis by DHPLC
`
`Bioline (2002-2005)
`
`Mutation detection enzymes
`
`Transgenomic (1999-2009)
`
`Establishment of a functional genomics
`laboratory
`
`Transgenomic (1999)
`
`Instrumentation for above
`
`Transgenomic (1999-2002)
`
`Staff budget
`
`BBSRC (1998-2001) with Dr J
`Grasby
`
`Use of SELEX to design novel enzyme
`activities
`
`Wellcome (1998-1999) with Dr
`Williams
`
`Catalytic DNA
`
`YCRC (1997-1998)
`
`Development of a novel PTT test
`
`Leukaemia Research Trust (1996-
`1998) with Dr K Ford (London)
`
`Targeting methylation for gene therapy
`
`12
`
`£38 000
`
`£1,450,200
`
`£597,160
`
`£135, 5812
`
`£250,000
`
`£59,087
`
`£50,000
`
`£60,000
`
`£60,000
`
`£800,000
`
`£400,000
`
`£600,000
`
`£120.000
`
`£50,000
`
`£20,000
`
`£96,000
`
`
`
`
`
`
`

`

`BBSRC (1995 – 1998 with Profs GM
`Blackburn and L Pearle (UCL)
`
`DNA repair enzymes
`
`Serono (1994-1995) with Dr CLR
`Barratt & Prof HD Moore
`
`Towards development of a
`contraceptive vaccine
`
`MRC (1993-1996) with Dr C Price
`
`DNA methylation in
`Schizosaccharomyces pombe
`
`MRC (1993-1996) with Prof H
`Moore and Dr C Barratt
`
`National Kidney Research Fund
`(1993-1995) with Dr SJ White
`
`SERC (1992-1995)
`
`Sheffield University Academic
`Development Fund (1992-1995)
`with Drs Engel, Barratt Profs Cooke
`& Bailey
`
`Characterisation of maturation factors
`that promote human sperm fertilization
`and developmental capacity
`
`Cloning of potassium channels from the
`distal nephrone of the human kidney
`
`
`Structure and function of a Nuclear
`DeoxyNTPase
`
`Comparative enzymology of DNA
`Methyltransferases Hgal M1 and M2
`
`SERC (1990-1993)
`
`Protein engineering of cytosine Specific
`DNA Methyltransferases
`
`SERC (1990-1993) with Prof GM
`Blackburn (Sheffield and Prof BA
`Connolly (Newcastle)
`
`YCRC (1990-1991)
`
`Wellcome Trust (1988-1991)
`
`YCRC (1988-1992)
`
`Cystic Fibrosis Trust (1988-1990)
`
`Molecular basis of adenine specific
`DNA methylation
`
`Molecular recognition of tumour
`promoters by protein kinase C
`
`Transcriptional control of rate hepatic
`malic enzyme gene
`
`Molecular studies on the Human DNA
`Methyltransferase gene
`
`Genetics of antibiotic resistance in
`Pseudomonas cepaciaI
`
`Nuffield Foundation (1986)
`
`Electrophoresis equipment grant
`
`Smith Kline Foundation (1986)
`
`Centrifuge equipment grant
`
`National Kidney Research
`Foundation (1996-98) Dr S White
`
`Control of expression of ROMK1
`variants in the kidney
`
`
`
`
`
`
`
`13
`
`£110,000
`
`£89,000
`
`£116,000
`
`£200,000
`
`£64,000
`
`£60,000
`
`£25,000
`
`£64,000
`
`£75,000
`
`£18,000
`
`£54,000
`
`£85,000
`
`£26,000
`
`£1,000
`
`£ 800
`
`£75,000
`
`
`
`

`

`Research staff and Collaborators
`
`
`Current staff
`
`Paul Brown (Senior Research Technician), Dr. Qaiser Sheikh (currently on secondment at the University of Sharjah),
`Yahya Aldawood( PhD year 4), Heba Alhamal (PhD year 4), Tom Grieves (PhD year 3) and three new PhD students
`from Libya (two jointly supervised with academics at the Liverpool School of Tropical Medicine)
`
`Former postdoctoral staff
`
`Around 20 postdoctoral staff have worked in my group and most hold academic or commercial positions in Science
`throughout the world.
`
`
`Postgraduate Training and Examination
`
`
`
`
`
`
`• Over 40 PhD students trained and graduated, many employed as academics: all engaged in research
`•
`Internal examiner for over 50 students and external examiner for over 40 students.
`
`Leadership, Management and Administration
`
`University 2007 to present
`
`Senior Committees
`
`
`• Research Services Review
`• External Relations Consultation Committee
`• Council Grievance Committee
`• Cross Cutting PVC Appointment Committee
`• Pay & Reward Review Group
`• HR Management Committee
`• Board of the Faculty of Pure Science
`• Member of the University Senate
`• Member of the University Council (2008-2011)
`• Institute of Cancer Studies Management Committee
`• Alumni Foundation Committee
`• HEIF 4 Management Group
`• Projects Working Group
`• University Health and Safety Management Group
`• Commercial Assessment Review Group (CAR)
`
`
`
`Departmental Committees
`
`
`• Chair of the Departmental Management Group
`• Teach