`
`InnoPharma Exhibit 1109.0001
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`

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`M. L, §immons
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`Prentice—«Hall, 1110.; Engiewoad Cliffs, N. I.
`
`InnoPharma Exhibit 1109.0002
`
`

`

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`Library of Cmgmm Cm‘amg Namim‘ F’s-93526
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`Primed in the: united States M America
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`InnoPharma Exhibit 1109.0003
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`

`

`Wish“ xicfmfna‘isimfim
`
`32‘?
`
`with
`poampamtive cam is; m nmka: certain the mice art: placed mm
`Cieaubedding and fresh food 2:316. water sch theiz’ immadiéite {ziwimnmmt wili
`in? {:ézzducive :3 quick healing and as, minimum {3f infacticms. The mice sitaulci
`be obxewm mgufarig and camfldly, partiwiariy ifclipa which can bECOEm':
`very I‘m-aemfhrtable to the animaL are useci CUBS (321115ng apparent {iisu
`wmf‘ort gem bar: rammed and mew ones; applied. In sum: cams, prophyfactic
`therapy may be infiiicamd {wing E8 imam: inflections anti mm: expminwnial
`reamna. {)xytafracydim or what broad Syeatrmn antibiotics can be piaceé
`in the drinkingwatex a: men flushed into the Racism: site. We do not think
`that
`this; pmcwua‘e is ordinarily necessary or perhaps»: even dasimbfa A
`reagent gublicmicm on murme gplemgmmy ‘rapmting use of am elemmcautery
`deviiza instead of ligatian is; of interem {ERSEK, 1968}. Other surgiezfi we?»
`11iquesg.
`ESLI‘ESE‘I as; myasm-garya may be used and am pmbably the: methods 9f
`the future. The cryosurgicat approach requires oniy minimal Iigmimm.
`
`FLU I, D A BMINISTRATIQN
`
`intraperimneai
`
`intraperimmai adminimmfim} is ust far many different purpascs in the
`amuse. Aqesthetias, tum-31‘ transpmuis? experimwtat druga and many others
`are marine}? ifljecwd by We inh‘apetitoneai mum. The technique i3 simple
`0110::
`this baaic method of picking up and balding {he Fk’lfltlfis‘?
`is mastered‘
`We remmmend gently but firmly grasping the mause by Um skin at the back
`of the neck and careflmy gathering the slack skin bpiweam the thumb and
`farefiuger. Caution mum be EELka to avoid pumng the fikin €00 tightly and
`strangiing the mouse‘ With the skin held pmperEy, the: Imam can be lifted
`and tha mil ancimred between the small finger and paim of 1114:; hand. The
`mouse is 11m»; in a pmsitian ta ta eaaily injected by a number of router}. To
`inject intraparilouaatly, a {y-inah Z} is Efvgauga mafia is. adequatrz. The
`aim of [he net-2612 depends. mam {m the Viémsity 0fth mattirial being iiljt‘cicd
`than in} an}: miner masideratimz. The: nadir: simuld be introduced on a plane
`farming appz‘eximamiy 3 11‘3" angle with the abdaminaf surfaca and siighiiy
`to the right 01' Daft of 1m: midiiam. The angie is important because it is easy fit)
`imam tin: mm:er info the urinary bladdgr car mm the intestine if the thigh: i5
`me great. With 30111:: practice, this {echlfique Ea easily mastered.
`
`Inwamuscular
`
`Intramuaeufar injectims in mice are fairiy diffimflt hmansa 0f rim Is.th (if
`a large: muscle mass. The usual site for intramuscufar injections L3
`in 1316
`
`InnoPharma Exhibit 1109.0004
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`

`

`l 23
`
`Tecfmiqtags
`
`prlzgerior 83;th mlmclggrmp. All-inch Eé-gaugqgmdk: i5 adequate. The same
`general restraining techniqna tan be mad if" anlysme parson is (bring the.
`mark. Other metlmds 0f restraint far intramuficular injections include plastic
`tubgs with halal cut in them large enmlgh 1-2:» allow ills lag and thigh t0 be
`gcnlly pulled through and injected.
`(Damian must be used in iniramuscular
`injfiatimas that the malls is :10: 91.13th la deep or 100 hard, far it may pass
`campletely thmugh the muscle mass. The madle slmulcfi be dimctetl perpen-
`dicular to the Sagitial plane... 03' pointed in a very slightly posterity: clireciian.
`
`Suhamtaneuus
`
`Subcutaneous injections in mice are prabably the easieaal {if all to give.
`The mama can be injectficl subcutaneouslly on Either the
`0|: the ventral
`Silk:: and a :lg-inch 22~gauge nasdle is adgquate far mast preparmiong. If the
`quantity of injmied material is fairly large 21ml {10:33 mm interfere with experi»
`mezztal pmmwl, multiple injection sites are advisable. The rate Ufaljswption
`is prebably reduced considfimbly from the fntraperitoneal er intramussular
`injealimng. As with Glhm‘ methods, tha regtraim 02" {ha animal is of great
`importance.
`
`Intratharacic
`
`lntrathoracic injectiuns: can he made in mice: with a slightly hem: or
`curved finch LEE-gauge media I: gilmllvzl be inserted between the ribs at
`approximately the: midpflint {3f the rib Gaga. Caution must be tat-1331 m insm‘i
`it at an angle, thus. preventing injection dirently inm 11mg tissue.
`lmmthomcic
`methods are net used rmtlnaly, and, unless there is a specific exparimemal
`reason is use tine mathed, the intraperimneal route 33 easier and the speed 9f
`amerptian is; similar.
`
`Intravemus
`
`The usual sit-l: of intravenous injecllcsn in mice iswlfge lateral tail vein.
`The mice can be rastraimed in a number of difieram’. ways. The simplest is m
`pass the tail through a limb slm in a small plasltlc shialcl.
`lf the animals
`have been warmed under light bulbs fm‘ appraximalaly ll} minis the wins will
`haw: expanded and inlravenous infiectiam can behacmmplislhed using, a
`lag-incl“: 24~gauga needliz.
`lt is a tachnique mm requires practice. but can be
`dorm {bulinely once: ihe akill
`is devaloped. As with all material
`injected
`intmwmusly, ll slmuld not contain exlmnwus maéerial that may 211:: as 311
`embalism and kill the animal.
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`InnoPharma Exhibit 1109.0005
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