`British Journal of Cancer (2004) 90(Suppl
`e
`© 2004 Cancer Research UK All rights reserved 0007- 0920/04
`$25.00
`www.bjcancer.com
`
`Fulvestrant and the sequential endocrine cascade for advanced
`breast cancer
`
`
`
`
`
`S Johnston”!
`‘Department of Medicine (Breast Unit), The Royal Marsden Hospital NHS Trust & Institute of Cancer Research, Fulham Road, London SW3 6{f UK
`
`
`is common for patients to
`it
`Following relapse on endocrine therapy for advanced, hormone receptor-positive breast cancer,
`experience responsesto alternative endocrine agents. Fulvestrant (‘Faslodex’) is a new type of endocrine treatment — an oestrogen
`receptor (ER) antagonist with no agonist effects. Fulvestrant downregulates cellular levels of the ER resulting in decreased expression
`of the progesterone receptor. This unique mode of action means that it
`is important that fulvestrant is placed optimally within the
`sequence of endocrine therapies to ensure that patients gain maximum benefit. Fulvestrant has shown efficacy when used after
`progression on tamoxifen or anastrozole in postmenopausal women with advanced breast cancer. After progression on fulvestrant,
`subsequent endocrine treatments can produce responses in many patients, demonstrating that fulvestrant does not
`lead to
`crossresistance with other endocrine therapies. Responsesto fulvestrant have also been observed in patients heavily pretreated with
`prior endocrine therapy. Fulvestrant is a versatile endocrine agent that may be integrated into the therapeutic sequence prior to, or
`subsequent to, other hormonal therapies, and represents a valuable additional antioestrogen for the treatment of postmenopausal
`women with advanced breast cancer.
`
`British Journal of Cancer (2004) 90(Suppl 1), SIS5—S18. dorl0.1038/sj.bjc.6601632 www.bjcancer.com
`© 2004 Cancer Research U
`
`
`
`Keywords: breast cancer; endocrine therapy; sequencing; fulvestrant; 'Faslodex’
`
`The efficacy and tolerability advantages associated with the use of
`endocrine agents in the treatment of hormone receptor-positive
`advanced breast cancer have been clearly established in many
`clinical
`studies. However, despite an initial
`response, many
`patients will eventually experience disease progression and require
`further endocrine treatment options. In patients who respond to
`endocrine treatments, additional responses to further agents are
`common (Buzdar and Hortobagyi, 1998; Hortobagyi, 1998). This
`potential responsiveness to multiple endocrine therapies means
`that patients may continue to derive clinical benefit while avoiding
`the marked, and often distressing, adverse side effects associated
`with chemotherapy. This is a particularly important consideration
`in a predominantly elderly patient population who maybe least
`able to tolerate severe adverse events. Disease control
`is also
`important in this patient group for whom an absolute cure may not
`be achievable, and instead, prevention of disease progression and
`the maintenance of quality of life may be more important.
`The activity of sequential endocrine therapies is dependent upon
`them possessing different mechanisms of action.
`In this way,
`crossresistance between sequential therapies may be avoided.It is
`therefore important that, as new endocrine therapies with different
`mechanisms of action become available,
`they are integrated
`effectively into the sequential hormonal regimens toallow patients
`to derive maximum benefit.
`
`FULVESTRANT AND THE ENDOCRINE SEQUENCE
`CASCADE
`
`Fulvestrant (‘Faslodex’) is a new type of endocrine treatment - an
`oestrogen receptor
`(ER) antagonist with no agonist effects
`(Wakeling et al, 1991; Robertson et al, 2001). Fulvestrant binds
`to the ER but, due to its steroidal structure and long side-chain,
`induces a different conformational shape with the receptor to that
`achieved by the nonsteroidal antioestrogen tamoxifen. As a result
`of this, fulvestrant prevents ER dimerisation and leads to the rapid
`degradation of the fulvestrant- ER complex, producing the loss of
`cellular ER (Borras et al, 1996). As a result, fulvestrant (unlike
`tamoxifen) inhibits ER- DNA binding and produces abrogation of
`oestrogen-sensitive gene transcription (Dauvois et al, 1993).
`The unique mode ofaction of fulvestrant presents a useful
`addition to the endocrine agents currently available for use in
`sequential therapeutic regimens. Fulvestrant has been approved in
`the United States and Brazil
`for
`the treatment of hormone
`receptor-positive metastatic breast cancer
`in postmenopausal
`women with disease progression following antioestrogen therapy.
`An increasing numberof studies are demonstrating the versatility
`of fulvestrant for the treatment of advanced breast cancer (Howell
`et al, 2002; Osborne et al, 2002; Perey et al, 2002; Steger et al,
`2003a, b). An understanding and appreciation of these data will be
`important for determining the optimal placing of fulvestrant in the
`sequence cascade of hormonaltherapy.
`
`
`
`Efficacy post-tamoxifen
`
`*Correspondence: Dr SRD Johnston;
`E-mail: Stephen johnston@rmh.nthames.nhs.uk
`
`The efficacy of fulvestrant has been proven in two phase III trials
`conducted in postmenopausal patients with hormone-sensitive
`
`AstraZeneca Exhibit 2065 p. 1
`InnoPharma Licensing LLC v. AstraZeneca AB IPR2017-00904
`Fresenius-Kabi USA LLC v. AstraZeneca AB IPR2017-01910
`
`
`
`Fulvestrant and the sequential endocrine cascade
`S Johnston
`S16
`
`advanced breast cancer progressing on prior tamoxifen. In both
`these trials, the efficacy of fulvestrant was comparableto the highly
`selective,
`third-generation aromatase inhibitor (AI) anastrozole
`(‘Arimidex’) (Howell et al, 2002; Osborneet al, 2002). Fulvestrant
`is
`the only antioestrogen acting directly on ER that has
`demonstrated efficacy post-tamoxifen,
`illustrating the lack of
`crossresistance between these two therapies. This is in contrast to
`the selective oestrogen receptor modulators (SERMs) such as
`droloxifene,
`idoxifene,
`toremifene, and benzothiophene arzoxi-
`fene, all of which have shown minimal activity in tamoxifen-
`resistant disease (Johnston, 2001).
`
`Efficacy post-Al
`
`Endocrine therapy after progression on fulvestrant
`
`Two studies have provided evidence that the marked reduction in
`ER expression produced by fulvestrant
`is not associated with
`crossresistance to subsequent endocrine therapies (Howell, 2002;
`Vergote et al, 2003). These studies used the retrospective analysis
`of data derived from questionnaires sent to clinicians who were
`involved in trials of fulvestrant as first- or second-line therapy
`(Osborne et al, 2002; Robertson et al, 2002). This methodology
`imposes
`certain limitations on the analyses. However,
`the
`information obtained from these studies does provide further
`evidence with regard to establishing sequencing regimens.
`Responses to subsequent endocrine therapy in patients who
`progressed on fulvestrant or tamoxifen as first-line therapy for
`advanced disease have been examined in a retrospective analysis
`improved efficacy and tolerability, Als are
`Owing to their
`(Howell, 2002). The limitations of this analysis are illustrated by
`increasingly being used in thefirst-line treatment of breast cancer,
`the fact that while 170 patients derived CB on fulvestrant, follow-
`in both early and advanced disease (Nabholtz et al, 2000; ATAC
`up data on patients who received subsequent endocrine therapy
`Trialists’ Group, 2002; Mouridsen et al, 2003). Preclinical data
`were available for only 35 of these. In these patients, subsequent
`indicate that exposure to long-term oestrogen deprivation (similar
`endocrine therapy produced CB in 20 out of 35 (57%) patients,
`to that caused by Als) and subsequent development of acquired
`with Al-based therapy producing CB in 11 out of 22 (50%) patients
`resistance may be accompanied by adaptive increases in ER gene
`(Table 1). It is interesting to note that in those patients who failed
`expression and intercellular signalling, resulting in hypersensitiv-
`to derive CB from fulvestrant, subsequent endocrine therapy
`ity to low oestradiol levels (Jeng et al, 1998; Shim et al, 2000; Chan
`produced a similar numberof responses to those seen in patients
`et al, 2002; Martin et al, 2003). In this situation, tamoxifen may be
`whodid derive CB from fulvestrant (15 out of 35 (43%) and 20 out
`perceived as an agonist. Asaresult, it is important to establish the
`of 35 (57%), respectively; Table 2). While the selection of patients
`efficacy of fulvestrant after progression on Als. In vitro, fulvestrant
`and patient numbers included in this analysis are limited by the
`significantly inhibited the expression of genes such as c-myb and
`nature of the data collection, they do indicate that responses may
`c-myc in cells resistant to long-term oestrogen deprivation (Jeng
`be obtained with AIs and other endocrine therapies
`after
`et al, 1998) and may therefore be an appropriate therapeutic
`progression on fulvestrant.
`option after progression on Als.
`In another retrospective analysis, 186 patients in total derived
`Clinical data so far are limited, but preliminary results from an
`CB on second-line fulvestrant, although questionnaire-based
`ongoing phase II study have shown that fulvestrant produced
`follow-up data were available for only 54 of these patients (Vergote
`clinical benefit
`(CB, complete response (CR)-+ partial response
`et al, 2003). The results showed that treatment with endocrine
`(PR) + stable disease (SD) 24 weeks) in seven out of 17 (41%)
`therapy (predominantly Als) after second-line fulvestrant pro-
`patients who hadreceived, and had progressed on, prior treatment
`duced CB in 25 out of 54 (46%) patients and objective response
`with tamoxifen and an Al (Perey etal, 2002). These results suggest
`(OR, CR+ PR) in four out of 54 (7%) patients who obtained CB
`that in addition to producing responses after prior tamoxifen,
`with second-line fulvestrant (Table 1). In patients who failed to
`disease progression after anastrozole may not preclude subsequent
`derive CB from second-line fulvestrant, further endocrine therapy
`treatment with fulvestrant. Furthertrials in this setting are now in
`produced CB in 18 out of 51 (35%) patients and OR in one out of
`progress and are discussed later in this paper.
`51 (2%) patients (Table 2). Preliminary analyses demonstrated a
`median duration of response to subsequent therapy of 383 and 318
`days, for patients who did and whodid not derive CB from second-
`line fulvestrant,
`respectively. Further endocrine therapy after
`progression on fulvestrant
`is therefore a viable and effective
`therapeutic option, with responses seen in patients treated with
`
`Efficacy in heavily pretreated patients
`
`Many patients may receive a number of different endocrine
`therapies as well as chemotherapies during the course of their
`disease. Preliminary data are becoming available from centres
`using fulvestrant
`in Named Patient Programmes
`involving
`patients heavily pretreated with endocrine therapies including
`tamoxifen, anastrozole,
`letrozole,
`exemestane, and goserelin.
`In 67 postmenopausal women with metastatic breast cancer, 64
`of whom (96%) had progressed on one,
`two or three prior
`endocrine agents for advanced disease, fulvestrant produced CB in
`40 patients (60%) overall. A total of six patients (9%) derived a PR.
`Of these, one had received fulvestrant as first-line therapy for
`advanced disease,
`two had received fulvestrant as second-line
`therapy, and three had received it as third-line therapy. No
`objective responses were seen in patients receiving fourth-line
`fulvestrant therapy. This might suggest that fulvestrant produces
`better responses when given earlier in the treatment sequence
`(Steger et al, 2003a).
`Similar results have been obtained in a separate single-centre
`study. Postmenopausal women with metastatic breast cancer who
`had been heavily pretreated with prior hormonal
`therapy
`(including tamoxifen, Als, androgens, and high-dose oestrogens)
`and chemotherapy (including taxanes, capecitabine, doxorubicin,
`and cisplatin) were treated with fulvestrant; SD 224 weeks was
`achieved in eight out of 42 (19%) patients (Franco etal, 2003).
`
`Response to subsequent therapy in patients who derivedclinical
`Table |
`benefit (CB) from fulvestrant
`
`Numberofpatients
`
` CR PR SD 224 weeks PD Total
`
`Patients who derived CB from first-line fulvestrant
`Endocrine therapy total
`|
`2
`\7
`Aromatase inhibitors
`|
`|
`9
`Tamoxifen
`0
`|
`7
`Megestrol acetate
`0
`0
`|
`Medroxyprogesterone acetate
`0
`0
`0
`
`Patients who derived CB from second-line fulvestrant
`Endocrine therapy total
`Oo
`4
`2\
`Aromatase inhibitors
`0
`3
`16
`Megestrol acetate
`0
`|
`5
`
`5
`Ul
`2
`0
`2
`
`29
`27
`2
`
`35
`22
`10
`|
`2
`
`54
`46
`8
`
`(2002) with permission of Breast Cancer Research and
`Adapted from Howell
`Treatment (Vergote et ai, 2003). CR=complete response; PR= partial response;
`SD = stable disease; PD = progressive disease.
`
`British Journal of Cancer (2004) 90(Suppl 1), S$15-S18
`
`© 2004 Cancer Research UK
`
`AstraZeneca Exhibit 2065 p. 2
`
`
`
`Fulvestrant and the sequential endocrine cascade
`S Johnston
`S|7
`
`tamoxifen and megestrol acetate as well as Als such as anastrozole
`andletrozole.
`
`DISCUSSION
`
`It is important to be aware of the sequenceversatility of fulvestrant
`so that it may be effectively and appropriately incorporated into
`the endocrine sequence cascade. Fulvestrant has demonstrated
`efficacy in the
`treatment of postmenopausal women with
`advanced, hormone-sensitive breast cancer, with data indicating
`that fulvestrant exhibits this activity in both the post-tamoxifen
`
`Table 2 Response to subsequent therapy in patients who did not derive
`clinical benefit (CB) from fulvestrant
`
`Numberof patients
`
` CR PR SD 224 weeks PD Total
`
`Patients who did not derive CB from first-line fulvestrant
`Endocrine therapy total
`0
`3
`12
`Aromatase inhibitors
`0
`0
`8
`Tamoxifen
`0
`3
`2
`Megestrol acetate
`0
`0
`|
`Medroxyprogesterone acetate
`0
`0
`|
`
`20
`Ul
`7
`0
`2
`
`Patients who did not derive CB from second-line fulvestrant
`Endocrine therapy total
`0
`|
`\7
`33
`Aromatase inhibitors
`0
`|
`15
`26
`Megestrol acetate
`0
`0
`|
`5
`Medroxyprogesterone acetate
`0
`0
`|
`2
`
`35
`19
`12
`|
`3
`
`51
`42
`6
`3
`
`(2002) with permission of Breast Cancer Research and
`Adapted from Howell
`Treatment. (Vergote et ai, 2003). CR=complete response; PR= partial response;
`SD = stable disease; PD = progressive disease.
`
`First-line
`
`
`Tamoxifen
`
`Second-line Fulvestrant “Al
`
`Al
`Fulvestrant
`
`
`Al Tamoxifen Fulvestrant Tamoxifen
`
`Third-line
`
`Al Fulvestrant Tamoxifen Al
`
`MA
`
`Fulvestrant
`
`fulvestrant has
`and postanastrozole setting. More specifically,
`been shown to be at least as effective as anastrozole in women
`with
`hormone-sensitive
`disease who
`have
`progressed
`on
`first-line
`therapy (mainly tamoxifen)
`(Howell
`et
`al,
`2002;
`Osborne et al, 2002; Robertson et al, 2003), with preliminary
`data
`showing promising results
`after progression on Als
`(Perey et al, 2002). Fulvestrant has also been used in patients
`pretreated with several endocrine agents
`as well as chemo-
`therapy. In one report, an overall CB rate of 60% was obtained,
`although patients who were treated with fulvestrant earlier
`in the sequence appeared to obtain better
`responses
`than
`those whoreceived it after progression on three endocrine agents
`(Steger et al, 2003a).
`In patients who undergo disease progression on fulvestrant, the
`novel modeofaction of this new endocrine therapy ensures a lack
`of crossresistance to other current endocrine agents (Howell, 2002;
`Vergote et al, 2003). Thus,
`the early use of fulvestrant
`in the
`sequence of endocrine therapies may not limit later choices of
`endocrine therapy. Fulvestrant can,
`therefore, potentially be
`integrated into sequential endocrine regimens at a number of
`positions, including the second-line setting after tamoxifen, or,
`potentially, after Als (Figure 1). Endocrine therapies such as
`megestrol acetate or the steroidal Al exemestane may then be
`employed as necessary after progression on fulvestrant. In this
`way, the potentially most effective and well-tolerated agents are
`used earlier in the treatment sequence.
`To optimise the positioning of fulvestrant in the sequence of
`endocrine therapies, additional
`studies will be required to
`elaborate upon the data so far accrued. New phase II andIII
`clinicaltrials of fulvestrant in over 3000 patients are either planned
`or currently in progress (Table 3). These will investigate additional
`roles for fulvestrant in breast cancer therapy,either following prior
`nonsteroidal AI treatment or in combination with Als asfirst-line
`therapy. In addition,
`loading-dose fulvestrant regimens will be
`tested. Two randomised, controlled trials are comparing the
`efficacy and tolerability of fulvestrant vs exemestane in post-
`menopausal womenprogressing after long-term oestrogen depri-
`vation resulting from prior AI therapy. The primary aim of the
`Study Of Faslodex vs Exemestane with/without Arimidex (SOFEA)
`trial is to compare progression-free survival in patients who have
`progressed on a nonsteroidal AI, and who are subsequently treated
`with either
`fulvestrant plus continued anastrozole, or with
`fulvestrant alone. Secondary aims
`include a comparison of
`fulvestrant vs exemestane and an examination of biological
`
`markers of response. A further trial, the Evaluation of Faslodex
`Al = aromataseinhibitor
`vs Exemestane Clinical Trial
`(EFECT)
`is currently recruiting
`patients to assess the efficacy of fulvestrant vs exemestane in
`patients who have progressed on treatment with nonsteroidal Als.
`In addition, two trials (FACT and SWOG 226) will compare the
`efficacy of a combination of fulvestrant plus anastrozole with
`anastrozole alone in thefirst-line setting (Table 3). The results of
`
`Fourth-line=MA MA MA MA Tamoxifen MA
`
`
`
`
`
`
`
`
`
`MA = megestrol acetate
`
`the available
`fulvestrant within
`Proposed positions of
`|
`Figure
`endocrine therapies for the sequential
`treatment of postmenopausal
`women with hormone receptor-positive, advanced disease. Adapted from
`Carlson (2002) with permission of Breast Cancer Research and Treatment.
`
`Table 3 New phaseIl/Ill clinical trials of fulvestrant in over 3000 breast cancer patients
`
`
`Trial
`Phase
`Population
`Treatments
`Patients (n)
`
`Fulvestrant 250 mg
`Fulvestrant 250 mg
`Fulvestrant LD 250mg vs exemestane
`Fulvestrant LD 250mg + anastrozole vs exemestane
`Fulvestrant LD 250 mgtanastrozole vs anastrozole
`Fulvestrant 250 mg+anastrozole vs anastrozole
`Fulvestrant 250 mg+anastrozole vs anastrozole
`Fulvestrant HD vs tamoxifen
`
`
`
`
`
`89
`93
`660
`750
`558
`690
`120
`60
`
`
`
`
`
`NCCTG
`SAKK
`EFECT
`SOFEA
`FACT
`SWOG 226
`0057
`FAST
`
`|
`|
`
`|
`|
`
`|
`|
`|
`|
`
`
`
`Post-tamoxifen or post-Als
`
`Post-tamoxifen or post-Als
`Post-nonsteroidal Al
`Post-nonsteroidal Al
`First-line
`First-line
`Neoadjuvant
`Neoadjuvant
`
`NCCTG =North Central Cancer Treatment Group; SAKK=Swiss Group for Clinical Cancer Research; EFECT = Evaluation of Faslodex vs Exemestane Clinical Trial;
`SOFEA = Study Of Faslodex vs Exemestane with/without Arimidex, SWOG = Southwest Oncology Group; LD 250 mg = loading-dose schedule of fulvestrant: 500 mg day 0,
`250 mg days 14 and 28, fulvestrant 250 mg per monthly thereafter; HD = high-dose schedule of fulvestrant 750mg 2—3 weeks presurgery.
`
`© 2004 Cancer Research UK
`
`British Journal of Cancer (2004) 90(Suppl
`
`1}, $15-S18
`
`AstraZeneca Exhibit 2065 p. 3
`
`
`
`Fulvestrant and the sequential endocrine cascade
`S Johnston
`S18
`
`these will further define endocrine-sequencing
`trials such as
`strategies, particularly as Als move forward into the first-line or
`adjuvant settings.
`Currently available data therefore indicate that fulvestrant will
`be a useful therapeutic option that may extend the opportunity for
`
`using endocrine therapies before reliance upon cytotoxic che-
`motherapy is necessary. Fulvestrant is also a versatile endocrine
`therapy that maybe used at a variety of positions in the sequential
`use of endocrine therapy for postmenopausal women with
`advanced, hormone-sensitive breast cancer.
`
`REFERENCES
`
`ATACTrialists’ Group (2002) Anastrozole alone or in combination with
`tamoxifen versus tamoxifen alone for adjuvant treatment of postmeno-
`pausal women with early breast cancer:
`first results of the ATAC
`randomisedtrial. Lancet 359: 2131-2139
`Borras M,LaiosI, el Khissiin A, Seo HS, Lempereur F, Legros N, Leclercq G
`(1996) Estrogenic and antiestrogenic regulation of the half-life of
`covalently labeled estrogen receptor in MCF-7 breast cancer cells. J
`Steroid Biochem Mol Biol 57: 203-213
`Buzdar AU, Hortobagyi G (1998) Update on endocrine therapy for breast
`cancer. Clin Cancer Res 4: 527-534
`Carlson RW (2002) Sequencing of endocrine therapies in breast cancer -
`integration of recent data. Breast Cancer Res Treat 75(Suppl 1): S27- $32
`Chan CM, Martin LA, Johnston SR, Ali S, Dowsett M (2002) Molecular
`changes associated with the acquisition of oestrogen hypersensitivity in
`MCE-7 breast cancer cells on long-term oestrogen deprivation. J Steroid
`Biochem Mol Biol 81: 333-341
`Dauvois S, White R, Parker MG (1993) The antiestrogen ICI 182780
`disrupts estrogen receptor nucleocytoplasmic shuttling. J Cell Sci
`106(Part 4): 1377 - 1388
`Franco S$, Perez A, Tan-Chiu E, Frankel C, Vogel C (2003) Fulvestrant
`(Faslodex®) demonstrates clinical benefit in heavily pretreated post-
`menopausal women with advanced breast cancer: a single-center
`experience. Breast Cancer Res Treat 82(Suppl 1): S105 (abstract 429)
`Hortobagyi GN (1998) Progress in endocrine therapy for breast carcinoma.
`Cancer 83: 1-6
`
`Howell A (2002) Postmenopausal women with advanced breast cancer
`who progress on fulvestrant or
`tamoxifen retain sensitivity to
`further endocrine therapies. Breast Cancer Res Treat 76(Suppl 1): $72
`(abstract 251)
`Howell A, Robertson JFR, Quaresma Albano J, Aschermannova A, Mauriac
`L, Kleeberg UR, Vergote I, Erikstein B, Webster A, Morris C (2002)
`Fulvestrant, formerly ICI 182,780,
`is as effective as anastrozole in
`postmenopausal women with advanced breast cancer progressing after
`prior endocrine treatment. J Clin Oncol 20: 3396-3403
`Jeng MH, Shupnik MA, Bender TP, Westin EH, Bandyopadhyay D, Kumar
`R, Masamura S, Santen RJ (1998) Estrogen receptor expression and
`function in long-term estrogen-deprived human breast cancer cells.
`Endocrinology 139: 4164-4174
`Johnston SR (2001) Endocrine manipulation in advanced breast
`cancer:
`recent advances with SERM therapies. Clin Cancer Res 7:
`4376s - 4387s
`Martin LA, Farmer I, Johnston SR, Ali S, Marshall C, Dowsett M (2003)
`Enhanced estrogen receptor
`(ER) alpha, ERBB2, and MAPK signal
`transduction pathways operate during the adaptation of MCE-7 cells to
`long term estrogen deprivation. J Biol Chem 278: 30458 - 30468
`Mouridsen H, Gershanovich M, Sun Y, Perez-Carrion R, Boni C, Monnier
`A, Apffelstaedt J, Smith R, Sleeboom HP,Jaenicke F, Pluzanska A, Dank
`M, Becquart D, Bapsy PP, Salminen E, Snyder R, Chaudri-Ross H, Lang
`R, Wyld P, Bhatnagar A (2003) Phase III study of letrozole versus
`tamoxifen asfirst-line therapy of advanced breast cancer in postmeno-
`
`pausal women: analysis of survival and update of efficacy from the
`International Letrozole Breast Cancer Group. J Clin Oncol 21: 2101-2109
`Nabholtz JM, Buzdar A, Pollak M, Harwin W, Burton G, Mangalik A,
`Steinberg M, Webster A, von Euler M (2000) Anastrozole is superior to
`tamoxifen as first-line therapy for advanced breast cancer in post-
`menopausal women: results of a North American multicenter rando-
`mized trial. Arimidex Study Group. J Clin Oncol 18: 3758 - 3767
`Osborne CK, Pippen J, Jones SE, Parker LM, Ellis M, ComeS, Gertler SZ,
`MayJT, Burton G, Dimery I, Webster A, Morris C, Elledge R, Buzdar A
`(2002) Double-blind,
`randomized trial comparing the efficacy and
`tolerability of fulvestrant versus anastrozole in postmenopausal women
`with advanced breast cancer progressing on prior endocrine therapy:
`results of a North Americantrial. J Clin Oncol 20: 3386 - 3395
`Perey L, Thiirlimann B, Hawle H, Bonnefoi H, Ahern J, Pagani O,
`Goldhirsch A, Dietrich D (2002) Fulvestrant (‘faslodex’) as hormonal
`treatment
`in postmenopausal patients with advanced breast cancer
`progressing after treatment with tamoxifen and aromatase inhibitors.
`Breast Cancer Res Treat 76(Suppl 1): $72 (abstract 249)
`Robertson JF, Nicholson RI, Bundred NJ, Anderson E, Rayter Z, Dowsett M,
`Fox JN, Gee JM, Webster A, Wakeling AE, Morris C, Dixon M (2001)
`Comparison ofthe short-term biological effects of 7-alpha-[9-(4,4,5,5,5-
`pentafluoropentylsulfinyl)-nonylJestra-1,3,5,
` (10)-triene-3,17beta-diol
`(Faslodex) versus tamoxifen in postmenopausal women with primary
`breast cancer. Cancer Res 61: 6739 - 6746
`Robertson JF, Osborne CK, Howell A, Jones SE, Mauriac L, Ellis M,
`Kleeberg UR, Come SE, Vergote I, Gertler S, Buzdar A, Webster A,
`Morris C (2003) Fulvestrant versus anastrozole for the treatment of
`advanced breast carcinoma in postmenopausal women - a prospective
`combinedanalysis of two multicenter trials. Cancer 98: 229 - 238
`Robertson JFR, Howell A, Abram P, Lichinitser M, Elledge R (2002)
`Fulvestrant versus tamoxifen for the first-line treatment of advanced
`breast cancer (ABC) in postmenopausal women. Ann Oncol 13(Suppl 5):
`46 (abstract 1640)
`Shim WS, Conaway M, Masamura S, Yue W, Wang JP, Kmar R, Santen RJ
`(2000) Estradiol hypersensitivity and mitogen-activated protein kinase
`expression in long-term estrogen deprived human breast cancer cells in
`vivo. Endocrinology 141: 396-405
`Steger G, Bartsch R, Wenzel C, Pluschnig U, Locker G, Mader RM,Zielinski
`CC (2003a) Fulvestrant
`(‘Faslodex’) demonstrates clinical benefit
`in
`heavily pre-treated patients with metastatic breast cancer. Eur J Cancer
`1(Suppl 5): $135
`Steger G, Bartsch R, Wenzel C, Pluschnig U, Locker G, Mader RM,Zielinski
`CC (2003b) Fulvestrant beyond the second hormonal treatmentline in
`metastatic breast cancer. Proc Am Soc Clin Oncol 22: 20
`Vergote I, Robertson JFR, Kleeberg U, Burton G, Osborne CK, Mauriac L
`(2003) Postmenopausal women whoprogress on fulvestrant (‘Faslodex’)
`remain sensitive to further endocrine therapy. Breast Cancer Res Treat
`79: 207-211
`
`Wakeling AE, Dukes M, Bowler J (1991) A potent specific pure antiestrogen
`with clinical potential. Cancer Res 51: 3867 - 3873
`
`British Journal of Cancer (2004) 90(Suppl 1), S$15-S18
`
`© 2004 Cancer Research UK
`
`AstraZeneca Exhibit 2065 p. 4
`
`