`
`ACHN ATCC ® CRL-1611 rM Homo sapiens kidney; derived from meta
`
`ACHN (ATCC®CRL-1611™)
`Organism:~~. human I Tissue: .!ill!ru!y; derived from metastatic site: pleural effusion Disease: renal cell adenocarcinoma
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`
`r;(~CUMENTATIOt,;
`
`Permits and Rostrlctlons
`
`Viow Permits
`
`Organism
`
`Tissue
`
`Homo sapiens, human
`
`kidney; derived from metastatic site: pleural effusion
`
`Product Format
`
`frozen
`
`Morphology
`
`epithelial
`
`Culture Properties
`
`adherent
`
`Blosafety Level
`
`Biosafety classification is based on US Public Health Service
`
`~ •. it Is the responsibility of the customer to ensure that
`
`their facilities comply with biosafety regulations for their own
`
`countiy
`
`Disease
`
`Ago
`
`Gender
`
`Ethnicity
`
`renal coll adenocarclnorna
`
`22 years
`
`male
`
`Caucasiar1
`
`Applications
`
`ACHN may be of use for antiproliforativo studies using human
`interferons or inte,ieron inducers.
`
`Storage Conditions
`
`liquid nitrogen vapor temperature
`
`https://www.atcc.org/Prod ucts/ AII/C R L-1611 . aspx? &p= 1 &rel =general information#general inform a lion
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`1/1
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`Breckenridge Exhibit 1158
`Breckenridge v. Novartis IPR2017-01592
`CRL-1611-ACHN
`Page 1
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`6/20/2018
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`ACHN ATCC ® CRL-1611 ™ Homo sapiens kidney; derived from meta
`
`ACHN (ATCC®CRL-1611™ )
`Organism: f:t.Qm.Q._fil/~,_rumi;in I lissue: kidney; derived from metastatic site: pleural effusion Disease: renal cell adenocarclnoma
`
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`
`Derivation
`
`Th0 ACHN cell line was initiated in Novombor. 1979 from tho
`
`malignant pleural effusion of a 22-year-old Caucasian male with
`
`widely metastatic renal adenocarcinoma (autopsy confirmed).
`
`Cells were seeded directly to culture flasks In Eagle's MEM with
`10% FBS, thon maintained and passaged 150 days in flasks.
`Cells were then inoculated subcutaneously into nude mice. After
`
`4 weeks, palpable, locally invasive tumors were noted. Both the
`
`original cells (ACHN) and those recovered from nude mouse
`tumors were growlh-lnt,lbiled by human Interferons.
`
`Clinical Data
`
`22 years
`Caucasian
`male
`
`Tumorlgenlc
`
`Yes
`
`Effects
`
`Comments
`
`Yes, in nudo mice
`(Tumors developed within 21 days at 100% frequency (5/5) in
`nude mice Inoculated subcutaneously with 10(7) cells)
`
`Growth Is Inhibited by human interferon [Hogan, T. F., personal
`communication].
`
`https://www.atcc.org/Products/AII/CRL-1611.aspx?&p=1 &rel=characteristics#characteristics
`
`1/1
`
`Breckenridge Exhibit 1158
`Breckenridge v. Novartis IPR2017-01592
`CRL-1611-ACHN
`Page 2
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`
`
`6/20/2018
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`ACHN ATCC ® CRL-1611 ™ Homo sapiens kidney; derived from meta
`
`ACHN (ATCC® CRL-1611 ™)
`Organism: ~piens,.lli!!lliill I Tissue: .!ill!.ru!y: derived from metastatic site: pleural effusion Disease: renal cell adenocarcinoma
`
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`
`Complota Growth Medium
`
`Tho base medium for this coll lino is Al'CC-formulatod Eagle's
`Minimum Essential Medium, Catalog No. 30-2003. To make the
`complete growth medium, add the following components to the
`base medium: fetal bovine serum to a final concentration of 10%.
`
`Subculturing
`
`Volumes are given for a 75 cm 2 flask. Increase or decrease t11e
`amount of dissociation medium needed proportionally for culture
`vessels of other sizes.
`1. Remove and discard culture medium.
`
`2. Briefly rinse the cell layer wit11 0.25% (w/v) Trypsin - 0.53 mM
`EOTA solution to remove all traces of serum which contains
`trypsin inhibitor.
`
`3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and
`observe cells under an Inverted microscope until cell layer Is
`dispersed (usually within 5 to 15 minutes).
`Note: To avoid clumping do not agitate the cells by hitting or
`shaking tho flask while waiting for the cells to detach. Cells
`that are difficult to detach may be placed at 37°C to facilitate
`dispersal.
`
`4. Add 6.0 to 8.0 ml of complete growth medium and aspirate
`cells by gently pipetting.
`
`5. Add appropriate aliquots of the cell suspension to new culture
`vessels.
`
`6. Incubate cultures at 37°C.
`
`Subcultivatlon Ratio: A subcultivation ratio of 1 :2 to 1 :3 is
`recommended
`Medium Renewal: 2 to 3 times per weak
`
`Cryopreservatlon
`
`Freeze medium: Complete growth medium, 95%; DMSO, 5%
`Storage temperature: liquid nitrogen vapor temperature
`
`Culture Conditions
`
`Atmosphere: air, 95%; carbon dioxide (CO2), 5%
`Temperature: 37°C
`
`https://www.atcc.org/Products/AII/CRL-1611 .aspx?&p= 1 &rel=culturemethod#culturemethod
`
`1/1
`
`Breckenridge Exhibit 1158
`Breckenridge v. Novartis IPR2017-01592
`CRL-1611-ACHN
`Page 3
`
`
`
`6/20/2018
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`ACHN ATCC ® CRL-1611 TM Homo sapiens kidney; derived from meta
`
`ACHN (ATCC®CRL-1611™)
`Organism: ~,mm§ • ..h.\unrul I Tissue: .!IB!ruly; derived from metastatic site: pleural effusion / Disease: renal cell adenocarclnoma
`
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`
`
`STR Profile
`
`Am0log0nin: X
`CSF1PO: 11
`DBS317: 12
`O16S539: 12,13
`O5S818: 12
`O7S820: 9,11
`THO1: 8
`TPOX: 8,11
`vWA: 16,17
`
`lsoenzymes
`
`G6PD, B
`
`https://www.atcc.org/Products/AII/CRL- 1611.aspx?&p=1 &rel=specifications#specifications
`
`1/1
`
`Breckenridge Exhibit 1158
`Breckenridge v. Novartis IPR2017-01592
`CRL-1611-ACHN
`Page 4
`
`
`
`6/20/2018
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`ACHN ATCC ® CRL-1611 TM Homo sapiens kidney; derived from meta
`
`ACHN (ATCC® CRL-1611™)
`Organism: Homo sapifil!li,..ill!l!lM. I llssue: kidru!Y.; derived from metastatic site: pleural effusion I Disease: renal cell adenocarcinoma
`
`HISTORY
`
`l':CCUMl:NTATICN
`
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`
`Name of Doposltor
`
`TF Hogan
`
`Deposltod As
`
`1-/omo sopiens
`
`Year of Origin
`
`November, 1979
`
`References
`
`Tumors developed within 21 d/Jys at 100% frequ0nc:y (5/5) In
`
`nude mice inoculated subcutanoousty with 10(7) col ts
`
`The ACHN cell tine was initiated In November, 1979 from the
`
`mallgnant pleural effusion of a 22-year-old Caucasian malo with
`
`widely metastatic renal adenocarcinoma (autopsy confirmed).
`
`Cells were seeded directly to culture flasks in Eagle's MEM with
`
`10% FBS, then maintained and passaged 150 days In flasks.
`
`Cells wero then inoculated subcutanoously into nude mice. After
`
`4 weeks, palpable, locally invasive tumors were noted. Both the
`
`original cells (ACHN) and those recovered from nude mouse
`
`tumors were growth-Inhibited by human Interferons.
`
`Kocl1evar J. Blockage of autonomous growth of ACHN cells by
`
`anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res.
`20: 2968-2972, 1990. PubMed: 2334900
`
`https://www.atcc.org/Products/AII/CRL-1611.aspx?&p=1 &rel=history#hlstory
`
`1/1
`
`Breckenridge Exhibit 1158
`Breckenridge v. Novartis IPR2017-01592
`CRL-1611-ACHN
`Page 5
`
`
`
`6/20/2018
`
`ACHN ATCC ® CRL-1611 TM Homo sapiens kidney; derived from meta
`
`ACHN (ATCC®CRL-1611™)
`Organism: HQmQ.fil!~,l:!.!.!rr!J!.n / lissue: .!ill!.!:!.!!y; derived from metastatic site: pleural effusion Disease: renal cell adenocarclnoma
`
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`
`Permits
`
`These permits may be required for shipping this product:
`
`Customers loc:atod In tho state) of Hawaii will neod to contact
`the Hawaii Department of Agriculture to determine if an Import
`Permit Is required. A copy of the permit or documentat1011 that
`a pormit is not roquired must bo sent to Al'CC in advance of
`shipment.
`
`Basic Documentation
`
`Product Sheet Certificate of AnalY.sls
`
`SOS
`
`References
`
`Tumors developed wit11in 21 days at 100% frequency (5/5) in
`
`nudu mice inoculated subcutaneously with 10(7) cells
`
`T11e ACHN cell line was initiated In November, 1979 from the
`
`malignant pleural effusion of a 22-year-old Caucasian male with
`
`widely metastatic renal adonocarcinoma (autopsy confirmed).
`
`Cells were seeded directly to culture flasks in Eagle's MEM with
`
`10% FBS, then maintained and passaged 150 days 111 flasks.
`
`Cells were then Inoculated subcutaneously into nude rnlce. After
`
`4 weeks, palpable, locally invasive tumors were noted. Both tho
`
`original cells (ACHN) and those recovered from nude mouse
`
`tumors were growth-inhibited by l1uman inte1fero11s.
`
`Kochevar J. Blockage of autonomous growth of ACHN cells by
`anti-renal cell carcinoma monoclonal antibody 5F 4. Cancer Res.
`
`20: 2968-2972, 1990. PubMed: 2334900
`
`https://www.atcc.org/Products/AII/CRL-1611.aspx?&p=1 &rel=documentation#documentation
`
`1/1
`
`Breckenridge Exhibit 1158
`Breckenridge v. Novartis IPR2017-01592
`CRL-1611-ACHN
`Page 6
`
`