A-498
`
`HyperCLDB
`
`18927105
`
`A-498 (human, kidney, carcinoma)
`
`Version 4.0.201701
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`P. Romano, A. Manniello et al. Nucl Acids Res
`For information, get in touch with: Dott.ssa
`Email:mariaassunta.manniello@hsanmartino.it
`2009 37:D925-D932.
`Assunta Manniello
`DOI: doi:10.1093/nar/gkn730; PMID:
`This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you
`will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g.,
`retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines).
`SPECIES: human
`TISSUE/ORGAN: kidney
`TUMOR: carcinoma
`DSMZ catalog code: ACC 55
`Morphology: epithelial-like, adherent cells growing as monolayers
`Description: human kidney carcinoma established from the kidney carcinoma of a 52-year-old man in 1973
`confirmed as human with IEF of AST, MDH, NP
`Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
`Depositor: Dr. H. Kirchner, Medical School of Hannover, Hannover, Germany
`General Restrictions
`Available in the following LABORATORY:
`German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
`90% MEM (with Earle's salts) + 10% FBS split confluent cultures 1:3 to 1:5 every 3-5 days using
`trypsin/EDTA; seed out at ca. 1.0 x 106 cells/80 cm 2 at 37C with 5% CO2 about 2-3 x 106 cells/80 cm 2;
`doubling time of > 60 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106
`cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI,
`microbiological culture, RNA hybridization, PCR assays;
`Validated by: cytogenetics 1 (hypertriploid with 7% polyploidy; 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9,
`-11, -14, +17, -18, -20, +21, +3m, dm/r (31%); der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q); sideline
`with der(3)t(3;3)(q27;p14)) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * immunological
`analysis (cytokeratin+ desmin- endothel- GFAP- neurofil- vimentin+) * isoenzymes (confirmed as human
`with IEF of AST, MDH, NP) * reverse transcriptase (negative)
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`http://bioinformatics.hsanmartino.it/cldb/cl164.html[3/29/2018 3:55:29 PM]
`
`NOVARTIS EXHIBIT 2088
`Breckenridge v. Novartis, IPR 2017-01592
`Page 1 of 2
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`A-498
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`See also
`
`Availability in cell line catalogues: ATCC: HTB 44, IZSBS: BS TCL 111, ICLC: HTL04005
`Bibliographic references:
`J Natl Cancer Inst 1973;51:1417-1423 - PMID: 4357758
`If you want to cite CLDB and HyperCLDB, please use the following
`For information, get in touch with:
`reference:
`Largo Rosanna Benzi, 10 - I-16132 Genova -
`ItalyDott.ssa Assunta Manniello
`Email:mariaassunta.manniello@hsanmartino.it
`
`P. Romano, A. Manniello, O. Aresu, M. Armento, M. Cesaro, B. Parodi.
`Cell Line Data Base: structure and recent improvements towards molecular authentication
`of human cell lines.
`Nucleic Acids Research 2009 37(Database issue):D925-D932.
`DOI: doi:10.1093/nar/gkn730; PMID: 18927105
`
`IRCCS AOU San Martino IST,
`c/o Centro Biotecnologie Avanzate, Torre D
`- Piano 2
`
`http://bioinformatics.hsanmartino.it/cldb/cl164.html[3/29/2018 3:55:29 PM]
`
`NOVARTIS EXHIBIT 2088
`Breckenridge v. Novartis, IPR 2017-01592
`Page 2 of 2
`
`