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`FAXCENTER
`AY 2 5 2005
`
`M
`
`Attorney Docket No.-: Enz-7(P)(C3)
`IN THE UNITED STATES PATENT AND TRADEMARK OFFIC ’
`.
`.
`.
`In re Application of:
`Janms Stavrianopoulos et al.
`Serial No.:
`08/486,070
`Group Art Unit: 1631
`Filed:
`June 7, 1995
`SPE: Ardin H. MarscheI,.TIh.D "'"T"'“""
`For:
`NUCLEIC ACIDS FIXED OR IMMOBIIJZED T0 NON-POROUS
`SOLID SUPPORT, AND SYSTEM. ARRAY AND NON-POROUS -
`GLASS OR PLASTIC SOLID SUPPORT COMPRISING SUCH FIXED
`OR IMMOBILIZED NUCLEIC ACIDS
`.
`.
`Confirmation No.:
`CERTIFICATE OF FACSIMILE TRANSMISSION
`
`7;)
`O _<_
`:5 53- V”
`m —<
`C‘)
`<5
`4:
`2 '23 V”
`(D ‘En C3
`
`Commissioner for Patents
`P.0. Box 1450
`Alexandria, VA 22313-1450
`
`Sir:
`
`I hereby certify that the attached correspondence (total of 137 pages
`
`including this sheet) comprising:
`Amendment Under 37 C.F.R. §1.115
`(In Response To November 26, 2004 Office Action) [including Exhibits A, B & C];
`
`and Request For Extension Of Time (3 Months)
`was sent to the United States Patent and Trademark Office by telefax to (703)
`
`872-9306.
`
`Date: May 25, 2005
`
`mitted,
`
`onald C. Feclus, Reg. No. 32,567
`Natalie Bogdanos, Reg. No. 51,480
`Attorneys for Applicants
`Enzo Life Sciences, Inc.
`clo Enzo Biochem, Inc.
`527 Madison Avenue, 9th Floor
`New York, NY 10022-4304
`Telephone (212) 583-0100
`Facsimile (212) 583-0150
`
`Enz—7(P)(C3)
`
`1'1
`
`Page 1 of 100
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`BD Exhibit 1013
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`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
` ) RECEIVED
`Applicant(s):
`Stavrianopoulos, et al.
`)
`°E"TFW- F‘-X GENE“
`Serial No.:
`O8/486,070
`;
`MAY .2 5 2005
`Group Art Unit: 1631
`Filed:
`June 7, 1995
`§
`SPE: Ardin H. Marschel, Ph.D.
`1
`NUCLEIC ACIDS FIXED on IMMOBILIZED
`)
`TO NON-POROUS SOLID SUPPORT. AND
`svsrsnn, ARRAY AND NON-POROUS GLASS)
`on PLASTIC soup suppom‘ COMPRISING )
`sucu FIXED on IMMOBILIZED NUCLEIC AcIDs l
`
`For:
`
`527 Madison Avenue, 9"‘ Floor
`New York, NY 10022-4304
`
`May 25, 2005
`
`VIA FILED BY FA CSIMILE TRANSMISSION
`TO.‘ (703) 872-9306
`
`Mail Stop -- Amendment
`Commissioner for Patents
`
`P.0. Box 1450
`Alexandria, VA 22313-1450
`
`AMENDMENT UNDER 37 C.F.R. §1 .115
`(IN RESPONSE TO THE NOVEMBER 26, 2004 OFFICE ACTION)
`
`Dear Sirs:
`
`This paper (Amendment Under 37 C.F.R. §1.115) is responsive to the
`November 26, 2004 Office Action mailed in connection with the above-identified
`application. A response to the November 26, 2004 Office Action was initially due
`by February 26, 2004. This paper is accompanied by a Request For Extension 01‘
`Time (Three Months), and authorization for the fee therefor. Upon the expected
`granting of Applicants‘ extension request, the response deadline will be extended
`to May 26, 2005. Accordingly, this paper is being timely filed.
`
`Enz-7(P)(C3)
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`-
`
`Page 2 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`CERTIFICATE OF TRANSMISSION
`
`I hereby certify that this paper (Amendment Under 37
`c.F.R. §1.115 (In Response To The November 26, 2004
`Office Action) (along with any documents referred to as
`attached or enclosed) is being facsimile transmitted to
`(Fax No. 703-
`
`Reg. No. 32.557
`
`872-93OBl on May 2.5.
`
`{W 2's’ 205';
`Data
`
`Ronald
`
`. Fedus
`
`:
`
`Amendments to the Claims are reflected in the listing of claims which begins on
`
`page 3 of this paper.
`
`Remarks begin on page 45 of this paper.
`
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`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 3 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) —- May 25, 2005]
`
` : This listing of the claims below will replace all prior
`
`versions and listing of claims in this application.
`
`Listing of Claims:
`
`Claims 1-3143 (Canceled).
`
`Claim 3144.
`
`(Currently Amended} A non-porous solid support comprising one or .
`
`more aminelsi, hydroxyllsl or epoxidelsi thereon, wherein at least one single-
`
`stranded nucleic acid ig
`
`fixed or immobilized thereto in
`
`hybridizable form to said non-porous solid support via said one or more aminelsl,
`
`an
`
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`
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`
`
`
`Claim 3145.
`
`(Currently Amended) A non—porous solid support comprising one or
`
`more aminelsl, hydroxyllsi or epoxidelsl thereon‘ wherein at least one double-
`
`stranded nucleic acid ip fixed or immobilized theretee to said non—porous solid
`
`support via said one or more aminels), hydroxyllsl or epoxiclelsl, and wherein at
`least one nucleic acid strand of said at least one double-stranded nucleic acid has
`
`covalently attached thereto either:
`
`_
`
`ii)
`
`(iii
`
`a non—radioactive signaling moiety, or
`
`a non—radioactive bridging moiey covalently or non-covalently
`
`attached to a non-radioactive signaling moiety.
`
`
`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Pae 4 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3146.
`
`(Currently Amended) The non-porous solid support of e-laim claims
`
`3144, 9+ 3145, 3287, 3324 or 3325, wherein said non-porous solid support
`
`comprises glass or plastic.
`
`Claim 3147.
`
`(Currently Amended) The non-porous solid support of claim claims
`
`3144, et 3145, 3287, 3324 or 3325, wherein said non-porous solid support
`
`comprises a plate or plates, a well or wells, a microtiter well or microtiter wells, g
`
`depression or depressions, a tube or tubes, a cuvette or cuvettes, or a head or
`
` beads,
`
`Claim 3148-3149.
`
`(Canceled).
`
`Claim 3150.» (Currently Amended) The non-porous solid support of claim 3287 or
`3325
`comprising One
`or more amines .
`
`Claim 3151.
`
`(Currently Amended) The non-porous solid support of elaimpl__ai_rfi
`
`3144-,_e1'-3145,
`
`3287 3324 or 3325, wherein said non-porous solid support has
`,
`
`been treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3152.
`
`(Previously Presented) The non-porous solid support of claim 3151,
`
`wherein said surface treatment agent comprises an amine providing compound, an
`
`epoxy glue or solution, an acid solution, or ammonium acetate.
`
`Claim 3153.
`
`(Previously Presented) The non-porous solid support of claim 3151 ,
`
`wherein said blocking agent comprises Denhardt‘s solution.
`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser; No. 08/486,070 (Filed June 7, 1995)
`
`Page 5 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3154.
`
`(Currently Amended) The non-porous solid support of elai-m c_|agms;
`
`3144, er 3145 3287 3324 or 3325, wherein said
`
`fixation or
`
`“
`
`immobilization to said non-porous solid support is Covalent.
`
`(Currently Amended) The non-porous solid support of elai-as claims
`Claim 3155.
`3144, or: 3145, 3287, 3324 or 3325, wherein said
`fixation or
`
`immobilization to said non-porous solid support is non—covalent.
`
`Claim 3156.. (Canceled).
`
`Claim 3157.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`
`3145 3287 or 3325, wherein one strand of said at least one double-stranded
`
`nucleic acid is inelireetly fixed or immobilized to said non-porous solid support by
`
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`
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`
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`
`andtheother
`
`neeleie-aeiel strand of said at least one double—stranded nucleic acid is hybridized to
`
`, said one strand.
`
`Claim 3158.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3144 or 3324, wherein said nucleic acid eemprises i_§ DNA—er—RNA.
`
`Claim 3159.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3145, 3287 or 3325, wherein said nucleic acid eempr-lees i§ DNA .
`
`Claim 3160.
`
`(Previously Presented) The non-porous solid support of claim 3144,
`
`wherein said nucleic acid comprises a nucleic acid sequence complementary to a
`
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 6 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The non-porous solid support of claim claims
`Claim 3161.
`3145 or 3287, wherein one strand of said at least one double-stranded nucleic acid
`
`comprises a nucleic acid sequence of interest sought to be identified, quantified or
`
`sequenced.
`
`Claim 3162.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3144 or 3324, wherein said single-stranded nucleic acid is unlabeled.
`
`Claim 3163.
`
`(Currently Amended) The non-porous solid support of claim claims
`
`3144, as 3145, 3287, 3324 or 3325, wherein said non-porous solid support is
`
`transparent or translucent.
`
`(Currently Amended) The non-porous solid support of claim c:l_a@§
`Claim 3164.
`3145 or 3237, wherein said
` fi
`signaling moiety is quantifiable in or from a fluid or solution, said quantity being
`proportional to the amount or quantity of said label-elelabels non—radioactive
`
`'
`
`signaling moiety.
`
`(Currently Amended) The non-porous solid support of s-(aim gfiimg
`Claim 3165.
`3145 or 3287, wherein said non-porous solid support is transparent or translucent,
`and said
`non-radioactive si nalin moiet
`is
`quantifiable in or from a fluid or solution or in-er-threugla from said non-porous solid
`support, said quantity being proportional to the amount or quantity of said label-er
`labels non—radioactive signaling moiety.
`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 7 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26. 2004 Office Action) -- May 25, 2005]
`
`Claim 3166.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`non-radioactive
`3145. 3287 or 3325, wherein said
` m@y comprises a chromagen or a chromagenic compound,as—eelereel
`
`
`
`(Currently Amended) The non-porous solid support of elaim claims
`Claim 3167.
`3145, 3287 or 3325, wherein a non-radioactive signal from said nee-reelieaeti-ve
`e non-radioactive signaling moiety is quantifiable or detectable by
`photometric techniques
`
`Claim 3168.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3144 or 3324, comprising more than one single-stranded nucleic acid.
`
`(Currently Amended) The non-porous solid support of elaim _
`Claim 3169.
`3145 3287 or 3325, comprising more than one double-stranded nucleic acid.
`Q
`
`Claims 3170 - 3171 (Canceled).
`
`(Currently Amended) A system comprising a non-porous solid
`Claim 3172.
`support which comprises one or more amine(s), hydroxyus) or egoxide(s) thereon,
`wherein and at least one single-stranded nucleic acid is
`fixed or
`immobilized thereto in hybridizable form to said non— orous solid su
`ort via said
`one or more aminegs), hydro>_<yl(s) or egoxide(s).
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 8 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) A system comprising a non-porous solid
`Claim 3173.
`support which comprises one or more amine(s), hydroxylgs) or epoxidejs) thereon,
`wherein and at least one double-stranded nucleic acid is fixed or immobilized
`1;-her-eta-, to said non-porous solid support via said one or more amine(s), hydroxyl(s)
`or epoxide(s), and wherein a~t—least one nucleic acid strand of said double—stranded
`nucleic acid has covalently attached thereto either:
`
`(i)
`(ii)
`
`a non—radioactive signaling moiety, or
`a non-radioactive bridging moiety covalently or non—covalentIy
`attached to a non-radioactive signaling moiety.
`
`
`
`(Currently Amended) The system of elaim claims 3172‘ or 3173,
`Claim 3174.
`3279, 3280, 3281 or 3282, wherein said non-porous solid support comprises glass
`
`or plastic.
`
`(Currently Amended) The system of elaim claims 3172,_ or 3173,
`Claim 3175.
`3279, 3280, 3281 or 3282. wherein said non-porous solid support comprises a
`plate or plates, a well or wells, a microtiter well or microtiter wells, ggepI;s_sicip;
`depressions, a tube or tubes, a cuvette or cuvettes, or a bead or beads,-er—a—set—e£
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`
`(Canceled).
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`Stavrianopoulos et al., u.s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 9 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`‘
`
`9“
`
`(Currently Amended) The system of elaim claims 3172, ex: 3173,
`Claim 3179.
`3279, 3280, 3281 or 3282, wherein said non-porous solid support has been
`treated with a surface treatment agent, a blocking agent, or both.
`
`(Previously Presented) The system of claim 3179, whereinsaid
`Claim 3180.
`surface treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3181.
`
`(Previously Presented) The system of claim 3179. wherein said
`
`blocking agent comprises Denhardt‘s solution.
`
`(Currently Amended) The system of elairn claims 3172, as 3173,
`Claim 3182.
`3279, 3280, 3281 or 3282, wherein said
`fixation or
`immobilization to said non-porous solid support is covalent.
`
`(Currently Amended) The system of elaim claims 3172, er: 3173,
`Claim 3183.
`279 3280 3281 or 3282 wherein said
`fixation or
` __4
`immobilization to said non-porous solid support is non-covalent.
`
`Claim 3184.
`
`(Canceled).
`
`(Currently Amended) The system of elaim claims 3173, 3281 or
`Claim 3185.
`3282, wherein said one strand of said double-stranded nucleic acid is inelireetly
`fixed or immobilized to said non—porous solid support
` and the other nucleic acid strand of said
`double-stranded nucleic acid is hybridized to said one strand.
`
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`Stavrianopoulos et al., u.s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`‘
`
`Page 10 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3186.
`
`(Currently Amended) The system of elaim claims 3172 or 3280,
`
`wherein said nucleic acid is earner-ises-DNA—er—RNA.
`
`Claim 3187.
`(Currently Amended) The system of elaim claims 3173, 3281 or
`3282, wherein said nucleic acid i_s DNA .
`'
`
`(Previously Presented) The system of claim 3172, wherein said
`Claim 3188.
`nucleic acid comprises a nucleic acid sequence complementary to a nucleic acid
`sequence of interest sought to be identified, quantified or sequenced.
`
`(Currently Amended) The system of elaim claims 3173 or 3288,
`Claim 3189.
`wherein one strand of said at least one double—stranded nucleic acid comprises a
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3190.
`
`(Previously Presented) The system of claim 3172, wherein said
`
`single-stranded nucleic acid is unlabeled.
`
`(Currently Amended) The system of claim claims 3172, er 3173,
`Claim 3191 .
`3279, 3280, 3281 or 3282, wherein said non—porous solid support is transparent
`
`or translucent.
`
`(Currently Amended) The system of eleim claims 3173 or 3288,
`Claim 3192.
`non-radioactive signaling moiety is
`wherein said
`quantifiable in or from a fluid or solution, said quantity being proportional to the
`amount or quantity of said laleel—er—leleels non—radioactive signaling moiety.
`
`Enz-7(Pl(C3)
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 11 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The system of elaim claims 3173 or 3288,
`Claim 3193.
`wherein said non-porous solid support is transparent or translucent, and said nea-
`non-radioactive signaling moiety is quantifiable in or from
`a fluid or solution or in-er—tl+reegh from said&non—porous solid support, said quantity
`being proportional to the amount or quantity of said label-eelabels non-radioactive
`
`signaling moiety.
`
`(Currently Amended) The system of elaim claims 3173, 3281 or
`Claim 3194.
`3282, wherein said
`non-radioactive signaling moiety
`comprises a chromagen or a chromagenic compound
`--
`. . . . g
`-g
`-
`-v
`--
`-— -.
`-
`
`
`
`(Currently Amended) The system of elaim claims 3173, 3281 or
`Claim 3195.
`3282, wherein a non-radioactive signal from said
`non-radioactive signaling moiety is quantifiable or detectable by photometric
` ‘technique ,
`I.
`I1.
`'._
`
`Claim 3196.
`
`(Previously Presented) The system of claim 3172, comprising more
`
`than one single-stranded nucleic acid.
`
`(Currently Amended) The system of claim claims 3173, 3281 or
`Claim 3197,
`3282, comprising more than one double-stranded nucleic acid.
`
`Enz-7(P)(C3)
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`

`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`1‘
`
`llillll 39lld
`
`Page 12 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3198.
`nucleic acids
`
`(Currently Amended) An array comprising various single-stranded
`fixed or immobilized in hybridizable form to a non-
`
`
`
`(Currently Amended) An array comprising various double-stranded
`Claim 3199.
`fixed or immobilized to a non-porous solid
`nucleic acids
`support, wherein at least one nucleic acid strand of said various double-stranded
`nucleic acids comprises at least one non-radioactive elsemieaklalael signaling moiety
`which is quantifiable or detectable
`
`
`
`(Currently Amended) The array of elaim claims 3198L er 3199,
`Claim 3200.
`3300, 3301 , 3326 or 3327, wherein said non-porous solid support comprises glass
`
`or plastic.
`
`Claim 3201 . (Currently Amended) The array of elaim claims 3198‘ e+ 3199,
`3300, 3301, 3326 or 3327, wherein said non-porous solid support comprises a
`plate or glate , a well or wells, a microtiter well or microtiter wells, a depression or
`depressions, a tube or tubes, at cuvette or cuvettes, or a bead or beads,—er—a-set-45$
`I
`I
`I
`I
`
`Claim 3202 -3203.
`
`(Canceled).
`
`Enz—7(P)(C3)
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`Page 13 of 100
`
`

`
`0Z'l2(Iil55'l1ll1|lN0|l.llllll0 s 09l0lI89llZ1GlS0 x 9096Zl83SlNEl u0ll':lllX:l3'01dSll1llIlS xl3U1!1lU5!lliPO 0131593] PM E01873 900Zl9Zl9 1V G/lOll x'll3ll8l 35)Vd
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 13 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3204.
`3326 3327
`
`(Currently Amended) The array of claims 3198, 3199, 3300, 3301 ,
`
`comprising one or more amines .
`
`(Currently Amended) The array of elaim claims 3198, or: 3199,
`Claim 3205.
`3300, 3301, 3326 or 3327, wherein said non—porous solid support has been
`treated with a surface treatment agent, a blocking agent, or both.
`
`(Previously Presented) The array of claim 3205, wherein said surface
`Claim 3206.
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3207.
`
`(Previously.Presented) The array of claim 3205, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`(Currently Amended) The array of elaim claims 3198, 9% 3199,
`Claim 3208.
`3300, 3301 , 3326 or 3327, wherein said
`fixation or
`immobilization to said non—porous solid support is covalent.
`
`(Currently Amended) The array of elaim claims 34193, or 3199,
`Claim 3209.
`3300, 3301, 3326 or 3327, wherein said
`fixation or
`immobilization to said non-porous solid support is non—covalent.
`
`Claim 3210.
`
`(Canceled).
`
`Enz-7lP)(C3)
`
`8)
`
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`
`Page 14 of 100
`
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`
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`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 14 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The array of claim claims 3199 or 3301,
`Claim 3211.
`wherein ene-str-and the first strands of said double—stranded nucleic acids 94:
`5 are
`fixed or immobilized to said non-porous solid support by
`and the other nucleic ' ' '
`
`
`
`acid strands of said double-stranded nucleic acids are hybridized to said first
`
`strands.
`
`Claim 3212.
`
`(Currently Amended) The array of elaim claims 3198 or 3300,
`
`wherein said nucleic acids i§_eem1e¥i-se—DNA-er-RNA.
`
`Claim 3213.
`
`(Currently Amended) The array of elaim claims 3199 or 3301,
`
`wherein said nucleic acids E_ DNA .
`
`(Currently Amended) The array of claim claims 3198 or 3289,
`Claim 3214.
`wherein said nucleic acids comprise nucleic acid sequences complementary to
`nucleic acid sequences of interest sought to be identified. quantified or sequenced.
`
`(Currently Amended) The array of elaim claims 3199 or 3290,
`Claim 3215.
`wherein one strand of said double-stranded nucleic acids comprises a nucleic acid
`sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3216.
`
`(Currently Amended) The array of elaim claims 3198 or 3300,
`
`wherein said single-stranded nucleic acids are unlabeled.
`
`(Currently Amended) The array of elaim claims 3198,_e+= 3199,
`Claim 3217.
`3300, 3301 , 3326 or 3327, wherein said non-porous solid support is transparent
`
`or translucent.
`
`Enz-7(P)(C3)
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`
`Page 15 of 100
`
`

`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 03/486,070 (Filed June 7, 1995)
`
`Page 15 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November» 26, 2004 Office Action) -- May 25, 20051
`
`(Currently Amended) The array of s-laim claims 3199 or 3290,_
`Claim 3218.
`wherein said non-radioactive s-l=remieal—label signaling moiety is quantifiable in or
`from a fluid or solution, said quantity being proportional to the amount or quantity
`
`of said label or labels.
`
`(Currently Amended) The array of elaim giai_rn_s 3199 or 3290,
`Claim 3219.
`wherein said non-porous solid support is transparent or translucent, and said non-
`. radioactive el'-teas-ieal—l-aleel signaling moiety is quantifiable in or from a fluid or
`solution or in—er-tlareugh 1‘r_og1_ said non-porous solid support. said quantity being
`proportional to the amount or quantity of said label-er—labels non-radioactive
`
`signaling moiety.
`
`(Currently Amended) The array of elaim claims 3') 99. 3301, 3326 or
`Claim 3220.
`3327, wherein said non-radioactive eh signaling moiety comprises a
`chromagen or a chromagenic compound
`
`
`
`(Currently Amended) The array of elraim claims 3199, 3301 , 3326 or
`Claim 3221.
`3327, wherein a non-radioactive signal from said non-radioactive ehei=nieal—lalael
`signaling moiety is quantifiable or detectable by photometric techniques7
`
`
`
`Enz-7(P)lC3)
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`Stavrianopoulos et al., U.S. Pat. App). Ser. No. 08/486,070 (Filed June 7, 1995)
`
`3
`
`lllllll 30Vd
`
`Page 16 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 20051
`
`(Currently Amended) An array comprising various single-stranded
`Claim 3222.
`fixed or immobilized in hybridizable form to a non-
`nucleic acids
`porous solid support having wells or depressions
`
`
`
`(Currently Amended) An array comprising various double-stranded
`Claim 3223.
`fixed or immobilized to a non-porous solid support
`nucleic acids
`having wells or depressions, wherein at least one nucleic acid strand of each of
`said various double-stranded nucleic acids comprises at least one non-radioactive
`e signaling moiety which is quantifiable or detectable
`
`
`A _- —=
`-
`.
`I -:4.
`nllnc:
`-=
`
`
`
`(Currently Amended) The array of elaim claims 3222 or 3223,
`Claim 3224.
`wherein said non-porous solid support comprises glass or plastic.
`
`Claim 3225 — 3226.
`
`(Canceled).
`
`Claim 3227.
`
`(Currently Amended) ihe array of claims 3222 or 3223 ,
`wherein said non-porous solid
`
`support comprises one or more amines .
`
`Enz—7(P)(C3)
`
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`
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`Page 17 of 100
`
`

`
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`Stavrianopoulos et'al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`(BUZZ ifilld
`
`Page 17 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3228.
`
`(Currently Amended) The array of elaim claims 3222 or 3223,
`
`wherein said non-porous solid support has been treated with a surface treatment
`
`agent, a blocking agent, or both.
`
`Claim 3229.
`
`(Previously Presented) The array of claim 3228, wherein said surface
`
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3230.
`
`(Previously Presented) The array of claim 3228, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Claim 3231.
`
`(Currently Amended) The array of elaim claims 3222 or 3223,
`
`wherein said
`
`fixation or immobilization to said non-porous solid
`
`support is covalent.
`
`Claim 3232.
`
`(Currently Amended) The array of elaim claims 3222 or 3223,
`
`wherein said
`
`fixation or immobilization to said non-porous solid
`
`support is non—covalent.
`
`Claim 3233.
`
`(Canceled).
`
`(Currently Amended) The array of claim 3223, wherein ene-strand
`Claim 3234.
`the first strands of said double—stranded nucleic acids
`gr_e fixed or
`
`immobilized to said non-porous solid support
` and the other nucleic acid strands of said double-
`stranded nucleic acids are hybridized to said first strands.
`
`Enz-7(P)(C3)
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`
`

`
`llZ'lZi33l55‘WU|)N0|1l1llfl0s09l0989ZlZ1lllS3s90E6Zl81SlNGa0ll':lllXd3'0l.dSllillIlSul3W!11ll5!|:)PGlU9l59!l llldi01l2l?990llZl9Zl9lll(1Il3ll zclitzaard
`Stavrianopoulos et al., u.s. Pat. App). Ser. No. 08/486,070 (Filed June 7, 1995)
`’
`
`Page 18 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 20051
`
`Claim 3235.
`
`(Currently Amended) The array of claim 3222, wherein said nucleic
`
`acids are DNA.G9
`
`Claim 3236.
`
`(Currently Amended) The array of claim 3223, wherein said nucleic
`
`acids are DNA.
`
`(Currently Amended) The array of claim 3222, wherein said nucleic
`Claim 3237.
`acids comprise a gene seguence or pathogen seguence nueleieaeid-sequences
`l
`I
`.
`.|
`E.
`|
`I
`.l
`T I’
`
`(Currently Amended) The array of claim 3223, wherein one strand of
`Claim 3238.
`said double—stranded nucleic acids comprises a gene seguence or pathogen
`seguence
`
`seqaeneed.
`
`Claim 3239.
`
`(Canceled).
`
`Claim 3240.
`
`(Previously Presented) The array of claim 3222, wherein said single-
`
`stranded nucleic acids are unlabeled.
`
`Claim 3241 .
`
`(Currently Amended) The array of eleim claims 3222 or 3223,
`
`wherein said non—porous solid support is transparent or translucent.‘
`
`(Currently Amended) The array of claim 3223, wherein said non-
`Claim 3242.
`radioactive eh signaling moieg is quantifiable in or from a fluid or
`solution, said quantity being proportional to the amount or quantity of said la!ael—er-
`
`labels non-radioactive signaling moiety.
`
`Enz-7(P)(C3)
`
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`
`‘d
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`
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`Page 19 of 100
`
`

`
`lJZ'lIl$il95'll|lll)ll0|l.llllllCla09l0£89ZlZ1CllS3s90E6ZZ8lS|NCla0ll':lllX:l3'0l.dSll3llllS:l3Ul!llll5|W?ClUJ3l593] llldZ0l£l19900Zl9Zl9l.V(lll0ll zcuvzaovd
`Stavrianopoulos et al., us. Pet. App). Ser. No. 08/486,070 (Filed June 7, 1995)
`’
`
`Page 19 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The array of claim 3223, wherein said non-
`Claim 3243.
`porous solid support is transparent or translucent, and" said non-radioactive
`el=ier=aic—al-label signaling moiety is quantifiable in or from a fluid or solution or i++er-
`through figr_n_ said non-porous solid support, said quantity being proportional to the
`amount or quantity of said label-er—labels non-radioactive signaling moiety.
`
`Claim 3244.
`
`(Currently Amended) The array of claim 3223, wherein said non-
`
`radioactive ehemieal—laleel signaling moiety comprises a chromagen or a
`I
`
`chromagenic compound,
`.
`_ a: —.-a
`
` AIID:_A- _
`'
`
`I C
`
`(Currently Amended) The array of claim 3223, wherein a non-
`laim 3245.
`radioactive signal from said non-radioactive ehemieal-laleel signaling moiey is
`quantifiable or detectable by photometric techniques
`
`
`(Currently Amended) A non-porous glass or non-porous plastic solid
`Claim 3246.
`support comprising one or more amine(s), hydroxy|(s) or egoxide(s) thereon,
`wherein at least one single-stranded nucleic acid E eli+eetly—er-inelir-eetly fixed or
`immobilized thereto in hybridizable form to said non-porous solid support via said
`I
`I
`.
`I
`.
`I
`.
`I
`:-
`-
`
`one or more amine s
`
`h drox I s or e oxide s
`-
`-
`-....= -.
`,.
`
`
`n
`
`.
`
`n
`.
`
`I
`
`I
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`
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`
`

`
`0Z'9l33l93'|1lUll N0l.l.VllllC| a 09)0£89Z)ZiCllS0 x90l36Zl85SlNll x llll':lllX:l3'0.l.d5ll1llllS alawll lll5!MPC| W593] llld Z0?9l?9 9llllZl9Zl9 1V (1ll0l|xl£ll9Z Hfillld
`Stavrianopoulos et al., US. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 20 [Amendment Under 37 C.F.R. _§1.1 15 (In Response To The
`Novemberv26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) A non-porous glass or non-gorous plastic solid
`Claim 3247.
`support comprising one or more aminelsl, hydroxylls) or epoxidegsl thereon,
`wherein at least one double-stranded nucleic acid i_s
`fixed or
`immobilized thel=ete7to said non- orous
`lass or non- orous
`Iastic solid su
`ort
`via said one or more aminelsl, hydroxyllsl or epoxidegsl, and wherein at-least one
`nucleic acid strand of said double-stranded nucleic acid has covalently attached
`
`thereto either:
`
`(I)
`(ii)
`
`a non-radioactive s

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