Stavrianopoulos et al.
`
`Serial No.:
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`08/486,070
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`Filed:
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`June 7, 1995
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`For: ARRAYS AND SYSTEMS COMPRISING
`ARRAYS FOR GENETIC ANALYSES AND
`01HER APPLICATIONS
`(As Previously Amended)
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`HAND CARRY
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`Mail Stop Amendment
`Commissioner for Patents
`P.O. Box 1450
`Alexandria, VA 22313-1450
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`Group Art Unit: 1631
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`Ex'r: Ardin H. Marschel, Ph.D.
`
`October 31, 2003
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`AMENDMENT UNDER 37 C.F.R. §1.115
`(FOLLOWING OFFICE ACTION OF JULY 2, 2003)
`
`Dear Sirs:
`
`This paper is directed to the July 2, 2003 Office Action. A response to the July 2, 2003
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`Office Action was originally due October 2, 2003. This paper is accompanied by a Petition for a
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`One-Month Extension of Time with a check in the amount of $110. Accordingly, this paper is
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`timely filed.
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`Please amend the above-identified Application as follows. Amendments to the Abstract
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`begin on page 2 of this paper. Amendments to the claims begin on page 3 of this paper.
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`Remarks begin on page 84 of this paper.
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`Enz-7(P)(C3)
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`BD Exhibit 1003
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`

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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 2 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`In The Abstract:
`
`Please delete the existing Abstract and replace it with the new Abstract shown in attached
`
`Appendix A.
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`Enz-7(P)(C3)
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`

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`' Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 3 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`In The Claims:
`
`Please cancel claims 1576-2160 and enter new claims 2161-3143.
`
`Claims 1-2160 (cancelled).
`
`Claim 2161. (NEW) A non-porous solid support comprising a single-stranded nucleic acid fixed
`or immobilized in hybridizable form thereto.
`
`Claim 2162. (NEW) The solid support of claim 2161, wherein said solid support comprises
`siliceous matter or polymeric material.
`
`Claim 2163. (NEW) The solid support of claim 2162, wherein said siliceous solid support
`comprises glass.
`
`Claim 2164. (NEW) The solid support of claim 2163, wherein said solid support comprises a
`plate, a well or wells, depressions, tubes, cuvettes, beads, or a set of said plates, wells,
`depressions, tubes, cuvettes or beads.
`
`Claim 2165. (NEW) The solid support of claim 2163, wherein said glass comprises a plate or a
`set of plates.
`
`Claim 2166. (NEW) The solid support of claim 2164, wherein said plate or plates comprise
`rnicrotiter well plates.
`
`Claim 2167. (NEW) The solid support of claim 2164, wherein said wells comprise rnicrotiter
`wells.
`
`Claim 2168. (NEW) The solid support of claim 2162, wherein said polymeric solid support
`comprises plastic.
`
`Claim 2169. (NEW) The solid support of claim 2168, wherein said plastic comprises
`polyethylene, polypropylene, polystyrene or polyepoxide.
`
`Claim 2170. (NEW) The solid support of claim 2168, wherein said plastic comprises a plate, a
`well or wells, depressions, tubes, cuvettes, beads, or a set of said plates, wells, depressions, tubes,
`cuvettes or beads.
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 4 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`Claim 2171. (NEW) The solid support of claim 2170, wherein said plate or plates comprise
`polystyrene plate(s).
`
`Claim 2172. (NEW) The solid support of claim 2170, wherein said wells comprise microtiter
`wells.
`
`Claim 2173. (NEW) The solid support of claim 2170, wherein said wells comprise polystyrene
`microfilter wells.
`
`Claim 2174. (NEW) The solid support of claim 2170, wherein said wells comprise removable
`wells.
`
`Claim 2175. (NEW) The solid support of claim 2161, wherein said solid support comprises
`more than one surface.
`
`Claim 2176. (NEW) The solid support of claim 2162, comprising reactive site(s) or binding
`site(s) thereon.
`
`Claim 2177. (NEW) The solid support of claim 2175, comprising reactive site(s) or binding
`site(s) on said more than one surface.
`
`Claim 2178. (NEW) The solid support of claim 2162, wherein said solid support has been
`treated with a surface treatment agent.
`
`Claim 2179. (NEW) The solid support of claim 2178, wherein said surface treatment agent
`comprises an amine providing compound, an epoxy glue or solution, an acid solution, or
`ammonium acetate.
`
`Claim 2180. (NEW) The solid support of claim 2179, wherein said amine providing compound
`comprises an amino-substituted hydrophobic polymer.
`
`Claim 2181. (NEW) The solid support of claim 2179, wherein said amine providing compound
`comprises dodecadiamine (DDA), polylysine (PPL), y-aminopropyltriethoxysilane or epoxy glue
`or solution.
`
`Claim 2182. (NEW) The solid support of claim 2179, wherein said amine providing compound
`comprises polylysine (PPL).
`
`Claim 2183. (NEW) The solid support of claim 2178, wherein said surface treatment agent
`comprises an epoxy glue or solution.
`
`Claim 2184. (NEW) The solid support of claim 2178, wherein said surface treatment agent
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 5 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`comprises an acid solution.
`
`Claim 2185. (NEW) The solid support of claim 2184, wherein said acid solution exposes a
`hydroxyl group or hydroxyl groups on said solid support.
`
`Claim 2186. (NEW) The solid support of claim 2176, wherein said reactive site(s) or binding
`site(s) comprise one or more amine, polyamine, amino-substituted or amino-derivatized groups
`thereon.
`
`Claim 2187. (NEW) The solid support of claim 2186, wherein said amine, polyamine, amino(cid:173)
`substituted or amino-derivatized groups result from treatment of said solid support with a surface
`treatment agent.
`
`Claim 2188. (NEW) The solid support of claim 2187, wherein said surface treatment agent
`comprises dodecadiamine (DDA), polylysine (PPL), y-aminopropyltriethoxysilane, or epoxy glue
`or solution.
`
`Claim 2189. (NEW) The solid support of claim 2161, wherein said nucleic acid is fixed or
`immobilized to an amine or polyamine on said solid support.
`
`Claim 2190. (NEW) The solid support of claim 2176, wherein said reactive site(s) or binding
`site(s) have been provided by a coating solution.
`
`Claim 2191. (NEW) The solid support of claim 2190, wherein said coating solution comprises
`dodecadiamine (DDA), polylysine (PPL), y-aminopropyltriethoxysilane, epoxy glue or solution,
`or acid solution.
`
`Claim 2192. (NEW) The solid support of claim 2176, wherein said reactive site(s) or binding
`site(s) comprise hydroxyl groups.
`
`Claim 2193. (NEW) The solid support of claim 2192, wherein said hydroxyl groups result from
`treatment of said solid support with an acid solution.
`
`Claim 2194. (NEW) The solid support of claim 2176, wherein said solid support has been
`treated with a blocking agent.
`
`Claim 2195. (NEW) The solid support of claim 2194, wherein said blocking agent comprises
`Denhardt's solution.
`
`Claim 2196. (NEW) The solid support of claim 2161, wherein said nucleic acid is fixed or
`immobilized directly to said solid support.
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 6 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`Claim 2197. (NEW) The solid support of claim 2196, wherein said direct fixation or
`immobilization to said solid support is covalent.
`
`Claim 2198. (NEW) The solid support of claim 2196, wherein said direct fixation or
`immobilization to said solid support is non-covalent.
`
`Claim 2199. (NEW) The solid support of claim 2161, wherein said nucleic acid is fixed or
`immobilized indirectly to said solid support.
`
`Claim 2200. (NEW) The solid support of claim 2199, wherein said indirect fixation or
`immobilization to said solid support is covalent.
`
`Claim 2201. (NEW) The solid support of claim 2199, wherein said indirect fixation or
`immobilization to said solid support is non-covalent.
`
`Claim 2202. (NEW) The solid support of claim 2161, wherein said nucleic acid is fixed or
`immobilized to said solid support by sandwich hybridization.
`
`Claim 2203. (NEW) The solid support of claim 2175, wherein said nucleic acid is fixed or
`immobilized to one of said reactive site(s) or binding site(s).
`
`Claim 2204. (NEW) The solid support of claim 2161, wherein said nucleic acid comprises
`DNA or RNA.
`
`Claim 2205. (NEW) The solid support of claim 2161, wherein said nucleic acid comprises a
`nucleic acid sequence complementary to a nucleic acid sequence of interest or sought to be
`identified or quantified or sequenced.
`
`Claim 2206. (NEW) The solid support of claim 2205, wherein said nucleic acid sequence of
`interest or sought to be identified or quantified or sequenced is isolated or derived from a
`biological or non-biological sample.
`
`Claim 2207. (NEW) The solid support of claim 2206, wherein said biological sample comprises
`blood, urine, feces, saliva, pus, semen, serum or tissue.
`
`Claim 2208. (NEW) The solid support of claim 2206, wherein said non-biological sample
`comprises fermentation broth or culture media.
`
`Claim 2209. (NEW) The solid support of claim 2205, wherein said nucleic acid sequence of
`interest or sought to be identified or quantified or sequenced comprises a gene or gene sequence,
`or a pathogen or pathogenic sequence.
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`

`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 7 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`Claim 2210. (NEW) The solid support of claim 2205, wherein said nucleic acid sequence of
`interest or sought to be identified or quantified or sequenced comprises one or more non(cid:173)
`radioactive chemical labels which comprise a non-radioactive signaling moiety or moieties which
`are quantifiable or detectable.
`
`Claim 2211. (NEW) The solid support of claim 2205, wherein said complementary nucleic acid
`sequence is unlabeled.
`
`Claim 2212. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label is the non-radioactive signaling moiety.
`
`Claim 2213. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label comprises a non-radioactive signaling moiety which is quantifiable in or from a fluid or
`solution or in or through said solid support, said quantity being proportional to the amount or
`quantity of said label or labels.
`
`Claim 2214. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label comprises a non-radioactive signaling moiety which is quantifiable in or from a fluid or
`solution or in or through said solid support, quantity being proportional to the amount or quantity
`of said label or labels.
`
`Claim 2215. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label is attached directly to said nucleic acid.
`
`Claim 2216. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label is attached directly to said nucleic acid.
`
`Claim 2217. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label is attached indirectly to said nucleic acid.
`
`Claim 2218. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label is attached indirectly to said nucleic acid.
`
`Claim 2219. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label is attached covalently to said nucleic acid.
`
`Claim 2220. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label is attached covalently to said nucleic acid.
`
`Claim 2221. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label is attached non-covalently to said nucleic acid.
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`Enz-7(P)(C3)
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`

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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 8 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`Claim 2222. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label is attached non-covalently to said nucleic acid.
`
`Claim 2223. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label is attached through one or more nucleotides in said nucleic acid.
`
`Claim 2224. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label is attached through one or more nucleotides in said nucleic acid strand.
`
`Claim 2225. (NEW) The solid support of claim 2223, wherein said attachment is indirectly
`through a bridging entity or a formation of a complex.
`
`Claim 2226. (NEW) The solid support of claim 2224, wherein said attachment is indirectly
`through a bridging entity or a formation of a complex.
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 9 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`Claim 2227. (NEW) The solid support of claim 2225, wherein said bridging entity or complex
`is covalently attached.
`
`Claim 2228. (NEW) The solid support of claim 2226, wherein said bridging entity or complex
`is covalently attached.
`
`Claim 2229. (NEW) The solid support of claim 2225, wherein said bridging entity or complex
`is non-covalently attached.
`
`Claim 2230. (NEW) The solid support of claim 2226, wherein said bridging entity or complex
`is non-covalently attached.
`
`Claim 2231. (NEW) The solid support of claim 2225, wherein said bridging entity or complex
`comprises biotin and avidin, biotin and streptavidin, a sugar and a lectin, or an antigen and an
`antibody.
`
`Claim 2232. (NEW) The solid support of claim 2226, wherein said bridging entity or complex
`comprises biotin and avidin, biotin and streptavidin, a sugar and a lectin, or an antigen and an
`antibody.
`
`Claim 2233. (NEW) The solid support of claim 2210, wherein said non-radioactive signaling
`moiety of said non-radioactive chemical label is covalently attached thereto.
`
`Claim 2234. (NEW) The solid support of claim 2210, wherein said non-radioactive signaling
`moiety of said non-radioactive chemical label is directly attached thereto.
`
`Claim 2235. (NEW) The solid support of claim 2210, wherein said non-radioactive signaling
`moiety of said non-radioactive chemical label is indirectly attached thereto.
`
`Claim 2236. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label comprises biotin, iminobiotin, a hapten or a ligand.
`
`Claim 2237. (NEW) The solid support of claim 2210, wherein said non-radioactive chemical
`label comprises an indicator molecule.
`
`Claim 2238. (NEW) The solid support of claim 2237, wherein said indicator molecule
`comprises a chromagenic compound, a fluorescent compound or a chemiluminescent compound.
`
`Claim 2239. (NEW) The solid support of claim 2212, wherein said non-radioactive chemical
`label comprises an indicator molecule.
`
`Claim 2240. (NEW) The solid support of claim 2239, wherein said indicator molecule
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 10 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`comprises a chromagenic compound, a fluorescent compound or a chemiluminescent compound.
`
`Claim 2241. (NEW) The solid support of claim 2210, wherein a quantifiable or detectable non(cid:173)
`radioactive signal is produced directly from said non-radioactive signaling moiety.
`
`Claim 2242. (NEW) The solid support of claim 2212, wherein a quantifiable or detectable non(cid:173)
`radioactive signal is produced directly from said at least one non-radioactive chemical label.
`
`Claim 2243. (NEW) The solid support of claim 2241, wherein said directly produced non(cid:173)
`radioactive signaling moiety comprises a chromagen, a fluorogen or a chemiluminescent
`compound.
`
`Claim 2244. (NEW) The solid support of claim 2242, wherein said directly produced non(cid:173)
`radioactive signaling moiety comprises a chromagen, a fluorogen or a chemiluminescent
`compound.
`
`Claim 2245. (NEW) The solid support of claim 2210, wherein said non-radioactive signaling
`moiety is indirectly produced.
`
`Claim 2246. (NEW) The solid support of claim 2212, wherein said non-radioactive signaling
`moiety is indirectly produced.
`
`Claim 2247. (NEW) The solid support of claim 2245, wherein said non-radioactive signaling
`moiety is indirectly produced by an enzyme or enzymatic reaction.
`
`Claim 2248. (NEW) The solid support of claim 2247, wherein said enzyme or enzymatic
`reaction comprises alkaline phosphatase, acid phosphatase, peroxidase, B-n-galactosidase or
`glucose oxidase.
`
`Claim 2249. (NEW) The solid support of claim 2247, wherein said enzyme or enzymatic
`reaction fluoresces or produces a color change upon contact with a chromagen.
`
`Claim 2250. (NEW) The solid support of claim 2249, wherein said chromagen comprises 4-
`methylumbelliferyl phosphate when said enzyme comprises alkaline phosphatase, wherein said
`chromagen comprises bis(4-methylumbelliferyl) phosphate, 3-0-methylfluroescein, flavone-3-
`diphosphate triammonium salt, or p-nitrophenyl phosphate 2Na when said enzyme comprises
`acid phosphatase, wherein said chromagen comprises tyramine hydrochloride, 3-(p(cid:173)
`hydroxyphenyl) propionic acid, p-hydroxyphenethyl alcohol, 2'2-azino-di-3-ethylbenzthiazoline
`sulfonic acid (ABTS), a-phenylenediamine 3HC1, o-dianisidine, 5-aminosalicylic acid, p-cresol,
`3,3'-dimethyloxybenzidine, 3-methyl-2-benozthiazoline hydrazone or tetramethyl benzidine
`when said enzyme comprises peroxidase, wherein said chromagen comprises o-nitrophenyl ~-D­
`galactopyranoside or 4-methylumbelliferyl-~-D-galactoside when said enzyme comprises ~-D-
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 11 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`galactosidase, and wherein said chromagen comprises 2'2-azino-di-3-ethylbenzthiazoline
`sulfonic acid (ABTS) when said enzyme comprises glucose oxidase.
`
`Claim 2251. (NEW) The solid support of claim 2246, wherein said non-radioactive signaling
`moiety is indirectly produced by an enzyme or enzymatic reaction.
`
`Claim 2252. (NEW) The solid support of claim 2251, wherein said enzyme or enzymatic
`reaction comprises alkaline phosphatase, acid phosphatase, peroxidase, E-n-galactosidase or
`glucose oxidase.
`
`Claim 2253. (NEW) The solid support of claim 2251, wherein said enzyme or enzymatic
`reaction fluoresces or produces a color change upon contact with a chromagen.
`
`Claim 2254. (NEW) The solid support of claim 2253, wherein said chromagen comprises 4-
`methylumbelliferyl phosphate when said enzyme comprises alkaline phosphatase, wherein said
`chromagen comprises bis(4-methylumbelliferyl) phosphate, 3-0-methylfluroescein, flavone-3-
`diphosphate triammonium salt, or p-nitrophenyl phosphate 2Na when said enzyme comprises
`acid phosphatase, wherein said chromagen comprises tyramine hydrochloride, 3-(p(cid:173)
`hydroxyphenyl) propionic acid, p-hydroxyphenethyl alcohol, 2'2-azino-di-3-ethylbenzthiazoline
`sulfonic acid (ABTS), a-phenylenediamine 3HC1, o-dianisidine, 5-aminosalicylic acid, p-c_resol,
`3,3'-dimethyloxybenzidine, 3-methyl-2-benozthiazoline hydrazone or tetramethyl benzidine
`when said enzyme comprises peroxidase, wherein said chromagen comprises o-nitrophenyl ~-D­
`galactopyranoside or 4-methylumbelliferyl-~-D-galactoside when said enzyme comprises ~-D­
`galactosidase, and wherein said chromagen comprises 2'2-azino-di-3-ethylbenzthiazoline
`sulfonic acid (ABTS) when said enzyme comprises glucose oxidase.
`
`Claim 2255. (NEW) The solid support of claim 2210, wherein sad non-radioactive signaling
`moiety comprises an enzyme, a co-enzyme, a chelating compound, a chromagenic compound, a
`fluorescent compound or a chemiluminescent compound.
`
`Claim 2256. (NEW) The solid support of claim 2212, wherein said non-radioactive signaling
`moiety comprises an enzyme, a co-enzyme, a chelating compound, a chromagenic compound, a
`fluorescent compound or a chemiluminescent compound.
`
`Claim 2257. (NEW) The solid support of claim 2210, wherein a non-radioactive signal is
`generated or generatable from said at least one non-radioactive chemical label by a means
`comprising chromagenic means, fluorescent means or chemiluminescent means.
`
`Claim 2258. (NEW) The solid support of claim 2212, wherein a non-radioactive signal is
`generated or generatable from said at least one non-radioactive chemical label by a means
`comprising chromagenic means, fluorescent means or chemiluminescent means.
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`Enz-7(P)(C3)
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`

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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 12 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`Claim 2259. (NEW) The solid support of claim 2210, wherein said at least one non-radioactive
`chemical label comprises a colored compound, a chemiluminescent compound or a fluorescent
`compound.
`
`Claim 2260. (NEW) The solid support of claim 2212, wherein said at least one non-radioactive
`chemical label comprises a colored compound, a chemiluminescent compound or a fluorescent
`compound.
`
`Claim 2261. (NEW) The solid support of claim 2259, wherein said colored compound
`comprises a dye.
`
`Claim 2262. (NEW) The solid support of claim 2260, wherein said colored compound
`comprises a dye.
`
`Claim 2263. (NEW) The solid support of claim 2210, wherein a non-radioactive signal from
`said non-radioactive signaling moiety is quantifiable or detectable by photometric means.
`
`Claim 2264. (NEW) The solid support of claim 2212, wherein a non-radioactive signal from
`said non-radioactive signaling moiety is quantifiable or detectable by photometric means.
`
`Claim 2265. (NEW) The solid support of claim 2263, wherein said photometric means
`comprise photometric techniques, spectrophotometric techniques, colorimetric techniques,
`fluorometric techniques or chemiluminescent techniques.
`
`Claim 2266. (NEW) The solid support of claim 2264, wherein said photometric means
`comprise photometric techniques, spectrophotometric techniques, colorimetric techniques,
`fluorometric techniques or chemiluminescent techniques.
`
`Claim 2267. (NEW) The solid support of claim 2161, wherein said solid support is transparent
`or translucent.
`
`Claim 2268. (NEW) A set comprising solid supports of claim 2161.
`
`Claim 2269. (NEW) A non-porous system which comprises solid supports of claim 2161.
`
`Claim 2270. (NEW) The system of claim 2269, wherein said system is transparent or
`translucent.
`
`Claim 2271. (NEW) The solid support of claim 2161, comprising various nucleic acids in
`hybridizable form fixed or immobilized thereto.
`
`Claim 2272. (NEW) The solid support of claim 2271, wherein said various nucleic acids
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 13 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`comprise an array.
`
`Claim 2273. (NEW) A non-porous solid support comprising reactive site(s) or binding site(s)
`thereon, and at least one single-stranded nucleic acid strand or sequence thereof fixed or
`immobilized in hybridizable form to said reactive site(s) or binding site(s).
`
`Claim 2274. (NEW) The solid support of claim 2273, wherein said solid support comprises
`siliceous matter or polymeric material.
`
`Claim 2275. (NEW) The solid support of claim 2274, wherein said siliceous solid support
`comprises glass.
`
`Claim 2276. (NEW) The solid support of claim 2275, wherein said glass comprises a plate, a
`well or wells, depressions, tubes, cuvettes, beads, or a set of said plates, wells, depressions, tubes,
`cuvettes, or beads.
`
`Claim 2277. (NEW) The solid support of claim 2275, wherein said glass comprises a plate or a
`set of plates.
`
`Claim 2278. (NEW) The solid support of claim 2276, wherein said plate or plates comprise
`microtiter well plates.
`
`Claim 2279. (NEW) The solid support of claim 2276, wherein said wells comprise microtiter
`wells.
`
`Claim 2280. (NEW) The solid support of claim 2274, wherein said polymeric solid support
`comprises plastic.
`
`Claim 2281. (NEW) The solid support of claim 2280, wherein said plastic comprises
`polyethylene, polypropylene, polystyrene or polyepoxide.
`
`Claim 2282. (NEW) The solid support of claim 2280, wherein said plastic comprises a plate, a
`well or wells, depressions, tubes, cuvettes, beads, or a set of said plates, wells, depressions, tubes,
`cuvettes or beads.
`
`Claim 2283. (NEW) The solid support of claim 2282, wherein said plate or plates comprise
`polystyrene plates.
`
`Claim 2284. (NEW) The solid support of claim 2282, wherein said wells comprise microtiter
`wells.
`
`Claim 2285. (NEW) The solid support of claim 2282, wherein said wells comprise polystyrene
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`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 14 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`microfilter wells.
`
`Claim 2286. (NEW) The solid support of claim 2282, wherein said wells comprise removable
`wells.
`
`Claim 2287. (NEW) The solid support of claim 2273, wherein said solid support comprises
`more than one surface.
`
`Claim 2288. (NEW) The solid support of claim 2287, comprising reactive site(s) or binding
`site(s) on said more than one surface.
`
`Claim 2289. (NEW) The solid support of claim 2273, wherein said solid support has been
`treated with a surface treatment agent.
`
`Claim 2290. (NEW) The solid support of claim 2289, wherein said surface treatment agent
`comprises an amine providing compound, an epoxy glue or solution, an acid solution, or
`ammonium acetate.
`
`Claim 2291. (NEW) The solid support of claim 2290, wherein said amine providing compound
`comprises an amino-substituted hydrophobic polymer.
`
`Claim 2292. (NEW) The solid support of claim 2290, wherein said amine providing compound
`comprises dodecadiamine (DDA), polylysine (PPL), y-aminopropyltriethoxysilane or epoxy glue
`or solution.
`
`Claim 2293. (NEW) The solid support of claim 2290, wherein said amine providing compound
`comprises polylysine (PPL).
`
`Claim 2294. (NEW) The solid support of claim 2289, wherein said surface treatment agent
`comprises an epoxy glue or solution.
`
`Claim 2295. (NEW) The solid support of claim 2289, wherein said surface treatment agent
`comprises an acid solution.
`
`Claim 2296. (NEW) The solid support of claim 2295, wherein said acid solution exposes a
`hydroxyl group or hydroxyl groups on said solid support.
`
`Claim 2297. (NEW) The solid support of claim 2273, wherein said reactive site(s) or binding
`site(s) comprise one or more amine, polyamine, amino-substituted or amino-derivatized groups
`thereon.
`
`Claim 2298. (NEW) The solid support of claim 2297, wherein said amine, polyamine, amino-
`
`Enz-7(P)(C3)
`
`

`

`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 15 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
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`substituted or amino-derivatized groups result from treatment of said solid support with a surface
`treatment agent.
`
`Claim 2299. (NEW) The solid support of claim 2298, wherein said surface treatment agent
`comprises dodecadiamine (DDA), polylysine (PPL), y-aminopropyltriethoxysilane, or epoxy glue
`or solution.
`
`Claim 2300. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence is fixed or immobilized to an amine or polyamine on said solid support.
`
`Claim 2301. (NEW) The solid support of claim 2273, wherein said reactive site(s) or binding
`site(s) have been provided by a coating solution.
`
`Claim 2302. (NEW) The solid support of claim 2301, wherein said coating solution comprises
`dodecadiamine (DDA), polylysine (PPL), y-arninopropyltriethoxysilane, epoxy glue or solution,
`or acid solution.
`
`Claim 2303. (NEW) The solid support of claim 2275, wherein said reactive site(s) or binding
`site(s) comprise hydroxyl groups.
`
`Claim 2304. (NEW) The solid support of claim 2303, wherein said hydroxyl groups result from
`treatment of said solid support with an acid solution.
`
`Claim 2305. (NEW) The solid support of claim 2273, wherein said solid support has been
`treated with a blocking agent.
`
`Claim 2306. (NEW) The solid support of claim 2305, wherein said blocking agent comprises
`Denhardt's solution.
`
`Claim 2307. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence is fixed or immobilized directly to said solid support.
`
`Claim 2308. (NEW) The solid support of claim 2307, wherein said direct fixation or
`immobilization to said solid support is covalent.
`
`Claim 2309. (NEW) The solid support of claim 2307, wherein said direct fixation or
`immobilization to said solid support is non-covalent.
`
`Claim 2310. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence is fixed or immobilized indirectly to said solid support.
`
`Claim 2311. (NEW) The solid support of claim 2310, wherein said indirect fixation or
`
`Enz-7(P)(C3)
`
`

`

`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 16 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
`
`immobilization to said solid support is covalent.
`
`Claim 2312. (NEW) The solid support of claim 2310, wherein said indirect fixation or
`immobilization to said solid support is non-covalent.
`
`Claim 2313. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence is fixed or immobilized to said solid support by sandwich hybridization.
`
`Claim 2314. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence is fixed or immobilized to one of said reactive site(s) or binding site(s).
`
`Claim 2315. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence comprises DNA or RNA.
`
`Claim 2316. (NEW) The solid support of claim 2273, wherein said nucleic acid strand or
`sequence comprises a nucleic acid sequence complementary to a nucleic acid sequence of interest
`or sought to be identified or quantified or sequenced.
`
`Claim 2317. (NEW) The solid support of claim 2316, wherein said nucleic acid sequence of
`interest or sought to be identified or quantified or sequenced is isolated or derived from a
`biological or non-biological sample.
`
`Claim 2318. (NEW) The solid support of claim 2317, wherein said biological sample comprises
`blood, urine, feces, saliva, pus, semen, serum or tissue.
`
`Claim 2319. (NEW) The solid support of claim 2317, wherein said non-biological sample
`comprises fermentation broth or culture media.
`
`Claim 2320. (NEW) The solid support of claim 2316, wherein said nucleic acid sequence of
`interest or sought to be identified or quantified or sequenced comprises a gene or gene sequence,
`or a pathogen or pathogenic sequence.
`
`Claim 2321. (NEW) The solid support of claim 2316, wherein said nucleic acid sequence of
`interest or sought to be identified or quantified or sequenced comprises one or more non(cid:173)
`radioactive chemical labels which comprise a non-radioactive signaling moiety or moieties which
`are quantifiable or detectable.
`
`Claim 2322. (NEW) The solid support of claim 2316, wherein said complementary nucleic acid
`sequence is unlabeled.
`
`Claim 2323. (NEW) The solid support of claim 2321, wherein said non-radioactive chemical
`label is the non-radioactive signaling moiety.
`
`Enz-7(P)(C3)
`
`

`

`Stavrianopoulos et al.
`Serial No.: 08/486,070
`Filed: June 7, 1995
`Page 17 [Amendment (Following Office Action of July 2, 2003)- October 31, 2003]
`
`Claim 2324. (NEW) The solid support of claim 2321, wherein said non-radioactive chemical
`label comprises a non-radioactive signaling moiety which is quantifiable in or from a fluid or
`solution or in or through said solid support, said quantity being proportional to the amount or
`quantity of said label or labels.
`
`Claim 2325. (NEW) The solid support of claim 2323, wherein said non-radioactive chemical
`label comprises a non-radioactive signaling moiety which is quantifiable in or from a fluid or
`solution or in or through said solid support, said quantity being proportional to the amount or
`quantity of said label or labels.
`
`Claim 2326. (NEW) The solid support of claim 2321, wherein said non-radioactive chemical
`label is attached directly to said nucleic acid.
`
`Claim 2327. (NEW) The solid support of claim 2323, wherein said non-radioactive chemical
`label is attached directly to said nucleic acid.
`
`Claim 2328. (NEW) The solid support of claim 2321, wherein said non-radioactive chemical
`label is attached indirectly to said nucleic acid.
`
`Claim 2329. (NEW) The solid support of claim 2323, wherein said non-radioactive chemical
`label is attached indirectly to said nucleic acid.
`
`Claim 2330. (NEW) The solid support of claim 2321, wherein said non-radioactive chemical
`label is attac