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`CENTRAL FAX CENTE
`
`p_
`vNo_715o
`I
`F#ESP0NSE UNDER 37 0 FR, 51, Z 26
`EXPEDITED PI-?0CED(JRE
`
`Attorney Docket No.: Enz-7(P)IC3)
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`In re Application of:
`
`Jannis Stavrianopoulos et ai.
`
`Serial No.:
`
`Filed:
`
`For:
`
`08/486,070
`
`June 7, 1995
`
`Group Art Unit: 1631
`
`Primary Exam'r: John S. Brusca
`
`NUCLEIC ACIDS FIXED OR IMMOBILIZED. T0 NON-POROUS.
`SOLID SUPPORT, AND SYSTEM, ARRAY AND NON-POROUS
`GLASS OR PLASTIC SOLID SUPPORT COMPRISING SUCH FIXED
`OR IMMOBILIZED NUCLEIC ACIDS
`'
`
`I
`
`Confirmation No.:
`
`CERTIFIOA TE OF FA CSIMILE TRANSMISSION
`
`Commissioner for Patents
`P.O. Box 1460
`Alexandria, VA 2231 3-1450
`
`Sir:
`
`I. hereby certify that the attached correspondence (total of 52 pages
`
`including this sheet) comprising:
`Supplemental Amendment To Applicants‘ November 2,, 2005 '
`Amendment Under 37 C.F.R. §1.116
`Iinciuding Exhibits A, B & C)
`
`was sent to the United States Patent and Trademark Office by telefax to (5712)
`
`273-8300..
`
`‘Deter November 8, 2005
`
`
`
`bmitted, R spectfully
`
`
`onaid C. Fedus, Reg. No. 32,567
`Natalie Bogdanos, Reg. No. 51,480
`Attorneys for Applicants
`Enzo Life Sciences, Inc.
`clo Enzo Biochem, Inc.
`527 Madison Avenue. 9th Floor
`New York, NY 10022-4304
`Telephone I212) 583-0100
`Facsimile (212) 583-0150
`
`Enz-7 IPI I03)
`
`PAGE 1I52 * RCVD III 11i8I20D5 4:50:40 PM {Eastern Standard Time)‘ ASVRIIISPTO-£FXRF«GJ2i ‘ DIIIS:2?33300 ‘ CSID:2125830150 ‘ DURATION (mm-ss)'1I-I2
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`Page 1 of 52
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`BD EXHIBIT 1024
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`p_
`NQ7150
`3 N35 UNDER 37 0 FR § 7 7‘
`CENTRALFAXCENTE g
`0 8
`GROUP ART UNIT 7632
`
`2
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`Group Art Unit: 1631
`
`Primary Examiner: John S. Brusca
`
`527 Madison Avenue, 9?“ Floor
`New York, NY 10022-4304
`November 8, 2005
`
`
`
`l l ) l l l l l l
`
`Applicantlsl:
`
`Stavrianopoulos, at al.
`
`Serial No.:
`
`08l486,07O
`
`Filed:
`
`For:
`
`June 7. 1995
`
`NUCLEICTACIDS FIXED 012 IMMOBJLIZED
`TO NON-POROUS soup supeorrr, AND
`
`svsrsm. ARRAY AND NON-POROUS GLASS .)
`on PLASTIC soup SUPPORT compmsme )
`sucu FIXED on lMMOBlLlZED NUCLEIC ACIDS )
`______.____.....___,%__..____)
`
`TVIA FILED BY F5 QSIMILE TRANSMISSIQM
`T0.’ (703) 872-9306
`
`Mail Stop -7 Non—Fee Amendment
`Commissioner fo'r_Patents
`P.O. Box 1.450
`Alexandria, VA 22313-1450
`
`‘
`
`SUPPLEMENTAL AMENDMENT TO APPLICANTS‘ EOVEMBER 2, 2005
`AMENDMENT UNDEB 37 C.F.R. §‘l .116
`
`Dear-Sirs:~
`
`This paper (Supplemental Amendment) follows Applicants‘ November 2,
`2005 Amendment Under 37 C.F.R. §1.116, the latter heving been filed in response
`
`to the Office Action mailed on September 29, 2005 in connection with the above-
`
`identified application. Their November 2, 2005 Amendment Under 37 C.F.R.
`§1.116 was timely filed. This paper (Supplemental Amendment) is also being
`
`timely filed with no extension request or fee being due.
`
`Enz-7lPl(C3l
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`PAGE 2152 ‘ RCVD AT lll8l2005 4:50:40 PM [Eastern Standard Time] ‘ SVR:USPTO-£FXRF-6127 ‘ DlllS:273330ll ‘ CSlD:212583015ll‘ DURATION (mm~ss)'10-12
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`Page 2 of 52
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`NO. 7160
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`P.3
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`U.S. Pat. Appl. Ser. No. 08/486,070 (Wed June 7, 1995}
`Stevrianopoulos et al.,
`To Applicants’ November 2, 2005
`Page 2 [Supplemental Amendment
`Amendment Under 37 C.F.R. §1.11 6]
`-- November 8, 2005]
`
`
`
`CERTIFICATE OF TRANSMISSION
`
`
`
`
`I hereby certify that this paper (Supplemental
`Amendment To Applicants‘ November 2. 2005
`Amendment Under 37 O.F.R. E1 .1 16 (along with any
`documents referred to as attached or enclosed) is being
`
`
`facsimile transmitted to the United States Patent and
`Trademarks {Fax.No. 571-2733300) on November 8.
`2005.
`Nov? zoos’
`
`
`Date
`,
`Ronaid C. Fedus
`Reg. No. 32.567
`
`
`
`
`
`
`
`
`
`Amendments to the Specification and a New Abstract Of The Disclosure are
`requested as reflected in Exhibits A and C accompanying this paper.
`
`Remarks begin on page 42 of this paper.
`
`Enz-7(PHC3)
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`PAGE 352 ‘ RCVD AT 11812005 4:50:40 PM [Eastern standard Time] ' ‘SVR:USPTO-EFXRF-612?’ DNIS2738300‘ CSlD:2125830150 ' DURATION (mm-ss):10-12
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 3 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`— November 8, 2005]
`
`4
`
`New Title of the invention:
`
`Please change the title to:
`
`-- SYSTEM, ARRAY AND NON-POROUS SOLID SUPPORT COMPRISIN
`
`FIXED OR IMMOBILIZED NUCLEIC? ACIDS —-
`
`.
`
`Amendmengg so the Specification:
`
`_
`
`Replace page ’1 of the specification with new Page 1 provided as Exhibit A to this
`
`paper.‘ A marked-up version of Page 1 is also provided as Exhibit A.
`
`_
`
`New Abstract Di‘ The Disclosure:
`Replace the former Abstract with the new Abstract Of The Disclosures provided as
`Exhibit C to this paper.
`’
`
`amendments to the Claims: This listing of the claims below will replace all prior
`versions and listing of claims in this application}
`
`Listing of Claims:
`
`Claims 1-3143 (Canceled).
`
`
`
`‘ An identical claim for priority was made in Applicants‘ March 7, 2001
`Amendment Under 37 C.F.R. §1.’l 15 {see page 2 of that paper, copy provided as
`Exhibit B).
`'
`’
`2 No claims are being amended, canceled or added by this paper.
`
`Enz-7(P)(C3)
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`PAGE 052 ‘ RCVD AT 111812005 4:50:40 Phi [Eastern Standard Time] ‘ SVR:USPTO<EFXRF-612? ‘ DNISQ738300 ' CSID’2‘|25830150 ‘ DURATION (mm-ss)'10 12
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (filed June 7, 1995i
`Page 4 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. E31 .116]
`—- November 8, 2005]
`
`Claim 3144.
`
`(Previously Presented) A non‘—porous solid support comprising one or
`
`more amine(s), hydroxyllsi or epoxldelsi thereon, wherein at least one single-
`
`stranded nucleic acid is fixed or immobilized in hybridizable form to said non-porous
`
`solid support via said one or more aminelsi, hydroxylisi or epoxidelsi.
`
`Claim 3145.
`
`(Previously Presented) A non-porous solid support comprising one or
`
`more amine(s), hydroxyl(s) or epoxidelsl thereon, wherein at least one double-
`
`stranded nucleic acid is fixed or immobilized to said non-porous solid support via
`
`said one or more amlnelsi, hydroxyl(s) or epoxidelsl, and wherein one nucleic acid
`
`strand of said at least one double-stranded nucleic acid has covalently attached
`
`thereto either:
`
`(i)
`
`(ii)
`
`.a non-radioactive signaling moiety, or
`
`a non~radioactive bridging moiety covalently or non-covalently
`
`attached to a non-radioactive signaling moiety.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`Claim 3146.
`3146, 3287, 3324 or 3325, wherein said non~porous solid support comprises glass
`
`or plastic.
`
`(Previously Presented) The norrporous solid support of claims 3144,
`Claim 3147.
`3143, 3287, 3324 or 3325, wherein said non-porous "solid support comprises a A
`A
`plate or plates, a well or wells, a microtiter well or microtiter wells, a depression or
`
`depressions, a tube or tubes, or a cuvette or cuvettes.
`
`Claims 31488149.
`
`(Canceled).
`
`Claim 3150.
`
`(Previously Presented) The non-porous solid support of claim 3287 or
`
`3325, comprising one or more amines.
`
`Enz-7lPllC3)
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`Page 5 of 52
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`Stavrlanopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 5 (Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.1‘l6]
`
`-- November 8, 2005]
`
`Claim 3151.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`
`3145, 3287, 3324 or 3325, wherein said non-porous solid support has been
`
`treated with a surface treatment agent, .a blocking agent, or both.
`
`Claim. 3152.
`
`(Previously Presented) The non-porous solid support of claim 3‘) 51,
`
`wherein said surface treatment agent comprises an amine providing compound, an
`
`epoxy glue or solution, an acid solution, or ammonium acetate.
`
`Claim 3153.
`
`(Previously Presented) The non—porous solid support of claim 31 51,
`
`wherein said blocking agent comprises Denharclt's solution.
`
`Claim 31 54.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`
`3145, 3287, 3324 or 3325, wherein said fixation or immobilization to said non-
`
`porous solid support is covalent.
`
`(Previously Presented) The non-porous solid support of claims 3144.
`Claim 3155.
`3145, 3287, 3324 or 3325, wherein said fixation or immobilization to. said non-
`
`porous solid support is non-covalent.
`
`' Claim 3156. ‘(Canceied).‘
`
`Claim 3157.
`(Previously Presented) The non—porous solid support of claims 3145,
`3287 or 3325, wherein one strand of said at least one double—stranded nucleic acid
`
`is fixed or immobilized to said non—porous solid support and the other strand of said
`
`at least one double—stranded nucleic acid is hybridized to said one strand.
`
`Enz-7{P)(C3i
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`Stavrianopouios et ai., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 6 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R.
`5.? 1 .1 16]
`-- November 8, 2005}
`
`7
`
`(Previously Presented) The non-porous solid support of claims 3144
`Claim 3158.
`or 3324, wherein said nucleic acid is DNA.
`
`Claim 3159.
`
`(Previously Presented) The non-porous solid support of claims 3145,
`
`3287 or 3325, wherein said nucleic acid is DNA.
`
`(Previously Presented) The non-porous solidsupport of claim 3144,
`Claim 3160.
`wherein said nucleic acid comprises a nucleic acid sequence complementary to a
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`(Previously Presented) The non-porous solid supportof ciaims:314-5,
`Claim 3161,
`or 3287, wherein one strand of said at least one doubiestranded nucleic acid
`comprises a nucleic acid sequence of interest sought to be identified, quantified or
`
`sequenced.
`
`(Previously Presented) The non-porous solid support of claims, 3144
`I Claim 3162.
`or 3324, wherein said single-stranded nucleic acid is unlabeled. ‘
`
`(Previously Presented) The non-porous solid support of claims 3144,
`Claim 3163.
`3145, 3287, 3324 or 3325, wherein said non-porous solid support is transparent
`
`' ortranslucent.
`
`(Previously Presented) The non-porous solid support of claims 3145
`Claim 3164.
`or 3287, wherein said non-radioactive signaling moiety is quantifiable in or from a
`fluid or solution, said quantity being proportional to the amount or quantity of said
`non—radioactive signaling moiety.
`'
`
`Enz-7(P)(C3)
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`Stavrianopoulos et al., U.S. Pat. App). Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 7 [Supplemental Amendment To Applicants‘ November .2, 2005
`V-
`Amendment Under 37 C.F.R. §'l.116]
`—- November 8. 2005]
`
`8
`
`(Previously Presented) The non-porous solid support of claims 3145
`Claim 3165.
`or 3287, wherein said non-porous solid support is transparent or translucent. and
`said non-radioactive signaling moiety is quantifiable in or from a fluid or solution or
`
`from said non-porous solid support, said quantity being proportional to the _amount
`
`or quantity of said non—ra’dioactive signaling moiety.
`
`(Previously Presented) The non-porous solid support of claims 3145,
`Claim 3166.
`3287- or 3325, wherein ‘said non-radioactive signaling moiety comprises a
`
`chromagen or e chromagenic compound.
`
`(Previously Presented) The non-porous solid support of claims 3145,
`Claim 3167.
`3287 or 3325, wherein a non-radioactive signal from said non-radioactive signaling
`
`moiety is quantifiable or detectable by photometric techniques.
`
`(Previously Presented) The non—porous solid support of claims 3144
`Claim 3168.
`or 3324, comprising more than one single-stranded nucleic acid.
`
`(Previously Presented) The non—porous solid support of claims 3145.
`Claim 3169.
`3287 or 3325, comprising more than one double-stranded nucleic acid.
`
`Claims 3170-3171 (Canceled).
`
`(Previously Presented) A system comprising a non-porous solid
`Claim 3172.
`support which comprises one or more aminelsl, hydroxyiisi or epoxidels) thereon,
`wherein at least one single-stranded nucleic acid is fixed or immobilized thereto in
`hybridizabie form to said non—porous solid support via said one or more aminels),
`
`hydroxylisi or epoxide(s).
`
`Enz—7(P)(C3l
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`"‘°E 3‘52’“°V° “T1W2°°54=5°¢4° Pi») Eastern Standard Timersvnzusmsrxmlzvomszzmzou*cslo:2125s3o1so*ouriA11ou (mm-ss)'lD-12
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 8 "(Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.Fl. §1.1'i6]
`—- November 8, 2006]
`
`9
`
`(Previously Presented) A system comprising a non—porous solid
`Claim 3173.
`support which comprises oneaor more aminelsl, hydroxyllsl or epoxidelsi thereon,
`wherein at least one double-stranded nucleic acid is fixed or immobilized to said
`non-porous solid, support via said one or more amine(s), hydroxylisi or epoxidelsi,
`and wherein one nucleic acid‘ strand of said double’-stranded nucleic acid has
`
`covalently attached thereto either:
`(i)
`a non-radioactive signaling moiety, or
`(ii)
`a‘ no‘n-radioactive"bridging moiety covalently or nomcovalently
`attached to a non~radioactive signaling moiety.
`
`(Previously Presented) The system of claims 3172, 31 73, 3279,
`Claim 3174.
`3280. 3281 or 3282, wherein said non-porous solid support comprises glass or
`
`plastic.
`
`(Previously Presented) The system of claims 3172, 3173, 3.279.
`Claim 3175.
`3280, 3281 or 3282, wherein "said non-porous solid support comprises a plate .or
`plates, at well or wells, at microtlter well or» rnicrotiter wells, a depression or
`depressions, a tube or tubes, or a cuvette or cuvettes.
`
`Claims 3176-3178.
`
`(Canceled).
`
`(Previously Presented) The system of claims 3172. 3173, 3279,
`Claim 3179.
`3280, 3281 or 3282, wherein said non~porous solid support has been treated with
`a surface treatment agent, a blocking agent. Of both-
`
`(Previously Presented) The system of claim 3179, wherein said
`Claim 3180.
`surface treatment agent comprises an amine providing compound, an epoxy glue or
`solution, an acid solution or ammonium acetate.
`
`Enz-7lP)(C3)
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`Stavrlanopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 9 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116l
`-- November 8, 2005]
`
`Claim 3181.
`
`(Previously Presented) The system of claim 3179, wherein said
`
`blocking agent comprises Denhardfs solution.
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3182.
`3280, 3281 or 3282. wherein said fixation or immobilization to said non-porous
`
`solid support is covalent.
`
`(Previousiy.Pr_esented) The system of claims 3172, 3173, 3279,
`Claim 3183.
`3280, 3281 or 3282, wherein said fixation or immobilization to said non-porous
`
`solid "support. is non-covalent.
`
`Claim 3184.
`
`(Canceled).
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`Claim 3185.
`wherein one strand of said doublestranded nucleic acid is fixed or immobilized to
`said non-porous solid support and the other nucleic acid strand of said doubie~
`stranded nucleic acid is hybridized to said one strand.
`
`(Previously Presented) The system of claims 3172 or 3280, wherein
`Claim 3186.
`said nucieie acid as DNA.
`
`Claim 3187.
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`
`wherein said nucleic acid is DNA.
`
`(Previously Presented) The system of claim 3172, wherein said
`Claim 3188.
`nucleic acid comprises a nucleic acid sequence complementary to a nucleic acid
`sequence of interest sought to be identified, quantified or sequenced.
`
`Enz-'7(P)(C3l
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`PAGE 105? ’ RCVD AT 1ii8i2U054:5i:40Pir1[Easiem Standard Time] ‘ Si/R:USPTO-EXRF-5l2? ’ DNlSI2?383li0 * CSlD:2125830l50 ’ DURATION (mm~ss):1ii-12
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 10 [Supplemental Amendment To‘ Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116l
`-- November 8. 2005]
`
`ll
`
`Claim 31 89.. (Previously Presented) The system of claims 3173 or 3288, wherein
`one strand of said at least one double-stranded nucleic acid comprises a nucleic
`acid sequence of interest sought to be identified, quantified or sequenced.
`
`(Previously Presented) The system of claim 3172, wherein said
`Claim 3190.
`single—stranded nucleic acid is unlabeled.
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3191.
`3280, 3281 or 3282, wherein said non—porous solid support is transparent or
`
`translucent.
`
`(Previously (Presented) The system of claims 3.173 or 3288. wherein
`Claim 3192.
`said non-radioactive signaling moiety is quantifiable in or from a fluid or solution,
`said quantity being proportional to the amount or quantity of said non-radioactive
`
`signaling moiety.
`
`(Previously Presented) The system of claims 3173 or 3288, wherein
`Claim 3193.
`said non-porous solid support is transparent or translucent, and said non—radioactive
`signaling moiety is quantifiable in or from a fluid or solution or from said non-porous
`solid support, said quantityibeiné proportional to the amount or qiuantity of said
`non—radioactive signaling moiety.
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`Claim 3194.
`wherein said non—radioactive signaling moiety comprises a chromagen or a
`
`chromagenic compound.
`
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`PAGE 11152‘ RCVD AT l1l8l20D5 4:50:40 Plil [Eastem Standard Time)’ SVRIUSPTO-EFXRF-5127 “ DNlS:27383fl0 ‘CSlD:2125830l50‘ DURATIOH (mmss):1li-12
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`V Stavrianopoulos et ai., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 11 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.1‘l6]
`—- November 8, 2006]
`
`i2
`
`Claim 3195.
`
`(Previously Presented) The system of claims 8173. 3281 or 3282,
`
`wherein a non—radioactive signal from said non-radioactive signaling moiety is
`
`quantifiable or detectable by photometric techniques.
`
`Claim 3196.
`
`lPreviously Presented) The system of claim 3172, comprising more
`
`than one single-stranded nucleic acid.
`
`Claim 3197.
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`
`comprising more than one double—stranded nucleic acid.
`
`(Previously Presented) An array comprisingvarious single-stranded
`Claim 31 98.
`nucleic acids’ fixed or immobilized in hybridizable form to a non-porous solid
`
`support.
`
`(Previously Presented) An array comprising various double-stranded
`Claim 3199.
`nucleic acids fixed or immobilized to a non—poro_us solid support, wherein at least
`one nucleic acid strand of said various double-stranded nucleic acids comprises at
`least one non-radioactive signaling moiety which is quantifiable or detectable.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 330') ,
`Claim 3200.
`3326‘or‘3327, wherein said non-porous solid support comprises glassor pl‘ast'ic‘.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 330.1,
`Claim 3201.
`3326 or 3327, wherein said non-porous solid support comprises a plate or plates, a
`well or wells, as mlcrotiter well or microtiter walls, a depression or depressions, a
`
`tube or tubes, or a cuvette or cuvettes.
`
`Claims 3202-3203.
`
`(Canceled).
`
`Enz~7(P)lC3l
`
`,
`,
`.
`.
`-
`~-
`PliGEm52‘RC
`l/DATiil8l20ll54.50.40Plviliastemsiandardlimei SVR.USPTO-EFXRF-iilli*DlllS.2?383il0*CSlD:2i25830ii0 DURATl0ll(mm-ss):10-12
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`ENZOBIOCHEM
`N0.7l60
`P.
`, U.S. Pat. Appi. Ser. No. 08/486,070 (Filed June 7, 1995)
`Stavrianopoulos et al.
`Amendment To Applicants‘ November 2, 2005
`Page 12 {Supplemental
`Amendment Under 37 C.F.R. §‘l .1 16]
`-- November 8, 20061
`
`13
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301 ,
`Claim 3204.
`3326 3327, comprising one or more amines.
`
`Claim 3205.
`
`(Previously Pres
`
`ented) The array of claims 3198, 3199, 3300, 3301,
`-porous solid support has been treated with a
`
`3326 or 3327, wherein said non
`surface treatment agent. a blocking agent, or both.
`
`_
`
`(Previously Presented) The array of claim 3205, wherein said surface
`Claim 3206.
`treatment agent comprises an amine providing compound, an epoxy glue or
`solution, an acid solution or ammonium‘ acetate.
`
`(Previously Presented) The array of claim 3205, wherein‘ said
`Claim 3207.
`blocking agent comprises Denhardt's solution.
`
`Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3208.
`(Previously
`n-porous solid
`3326 or 3327, wherein said fixation or immobilization to said no
`support is covalent.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3209.
`33.26 or 3327, wherein said fixation or immobilization to said’ non-porous solid’
`
`support is non-covalent.
`
`Claim 3210.
`
`(Canceled).
`
`Enz-7(P}(C3)
`
`PAGE 13l52 ' RCVD AT 1ll8l20ll54:5il:-10 Pill [Easlem Standard Time] ’ SVR:USPl'0-EFXRF-5l2? “ DlllS:2738300 ’ CSlD:2i25830l50* DURATl0ll (mm-ss):10-12
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 03/486,070 (Filed June 7. 1995)
`Page 13 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.‘l 16]
`-- November 8, 2005]
`
`(Previously Presented) The array of claims 3199 or 3301 , wherein
`Claim 3211.
`the first strands of said double—stranded nucleic acids are fixed or immobilized to
`said non-porous solid support and the other nucleic acid strands of said double-
`stranded nucleic acids are hybridized to said first strands.
`
`Claim 3212.
`
`(Previously Presented) The array of claims 3198 or 3300, wherein
`
`said nucleic acids is DNA.
`
`Claim 3213.
`
`(Previously Presented) The array of claims 3199 or 3301, wherein
`
`said nucleic acids is DNA.
`
`(Previously Presented) The array of claims 3198 or 3289, wherein
`Claim 3214.
`said nucleic acids comprise nucleic acid sequences complementary to nucleic acid
`'sequences of interest sought to be identified. quantified or sequenced.
`
`(Previously Presented) The array of claims 3199 or 3290, wherein
`Claim 3215.
`one strand of said double-stranded nucleic acids comprises a nucleic acid sequence
`of interest sought to be identified, quantified or sequenced.
`
`(Previously Presented) The array of claims 3198 or 3300, wherein
`Claim 3216.
`said single—strande‘d nucleic acids’ are unlabeled.
`
`(Previously Presentedi The array of claims 3198. 3199, 3300,3301,
`Claim 3217.
`3326 or 3327, wherein said non-porous solid support is transparent or translucent.
`
`(Previously Presented) The array of claims 3199 or 3290, wherein
`Claim 3218.
`said norwadioactive signaling moiety is quantifiable in or from a fluid or solution,
`said. quantity being proportional to the amountor quantity of said label or labels.
`
`Enz-7lP)l C3)
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`PAGE 1452‘ RCVD Al‘ 11l3l2U05l:50:i0Plll[Easlem standard lime] * SVRIUSPTO-EFXRF-5i27 ‘ DN|S:2l38300 * CSlD:2l25330l50 ' DURATION (mm-ss)'l0-12
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`Stavrlanopoulos et al.,
`Page 14 [Supplemental
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`P.
`N0. 7160
`ENZO BIOCHEM
`US. Pat. Appl. Ser. No. 08/486,070 (Filed June 7. 1995)
`Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.1i6]
`-- November‘ 8, 2005]
`
`)5
`
`(Previously Presented) The array of claims 3199 or 3290, wherein
`Claim 3219.
`said non-poroussolid support is transparent or translucent, and said non-radioactive
`signaling moiety is quantifiable in or from a fluid or solution or from said non—porous
`solid support, said quantity being proportional to the amount or quantity of said
`non-radioactive signaling moiety.
`
`(Previously Presented) The array of claims 3199, 3331, 3326 or
`Claim‘ 3220.
`3327, wherein said non-radioactive signaling moiety comprises a chromagen or a
`
`chromagenlc compound.
`
`(Previously Presented) The array of claims 3199, 3301.. 3326 or
`Claim 3221.
`.3327, wherein a non-radioactive signal from said non-radioactive signaling moiety
`is quantifiable or detectable by photometric techniques.
`
`(Previously Presented) An array comprising various single-stranded
`Claim 3222.
`nucleic acids fixed or immobilized in hybridizable fonn to a non-porous solid support
`
`having wells or depressions.
`
`(Previously Presented) An array comprising various double-stranded
`Claim 3223.
`nucleic acids ilxed or immobiilzedlto a non—porous solid support having’ wells or
`wherein at least one nucleic acid strand of each of said various
`one non-radioactive signaling
`
`depressions,
`double-stranded nucleic acids comprises at least
`moiety which is quantifiable or detectable.
`
`(Previously Presented) The array of claims 3222 or 3223. wherein
`Claim 3224.
`said non—porous solid support comprises glass or plastic.
`
`Enz-7lPl(C3)
`
`PAGE l5l52 ’ RCVD AT 1ll8l2005 4:50:40 PM [Easiem Standard Time] ' SVRIUSPTUEXRF-5l27 “ DlllS:27383fl0 ‘ CSlD:2125830i50 * DURATl0ll (mm-ss)'l0-12
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`Page 15 of 52
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 15 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.l-‘l. §1.116]
`-- November 8. 2005}
`
`Claims 322543226.
`
`(Canceled).
`
`Claim 3227.
`
`(Previously Presented) The array of claims 3222 or 3223, wherein
`
`said non~porous solid support comprises oneor more amines.
`
`(Previously Presented) The. array of claims 3222 or 3223, wherein
`Claim 3228.
`said non-porous solid support has been treated with a surface treatment agent, a
`
`blocking agent, or both.
`
`(Previously Presented) The array of claim 3228, wherein said surface
`Claim 3229.
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3230.
`
`(Previously Presented) The array of claim 3228, wherein said
`
`blocking agent comprises De-nhardt's solution.
`
`(Previously Presented) The array of claims 3222 or 3223. wherein
`Claim 3231.
`said fixation or immobilization to said non-porous solid support is covalent’.
`
`(Previously-Presented) The array of claims 13222 or 3223, wherein
`Claim 3232.
`saidifixation or immobilization to said norrporous solid supporfis non-covalent.
`
`Claim 3233.
`
`(Canceled).
`
`(Previously Presented) The array of claim 3223, wherein the first
`Claim 3234.
`strands of said double—stranded nucleic acids are fixed or immobilized to said non-
`porous solid support and the other nucleic acid strands of said doublestranded
`nucleic acids are hybridized to said first strands.
`
`‘En:-7u=lic3)
`
`PRGE 16152 ‘ RCVD AT 1118/2005 4:50:40 Pill [Eastern Standard Time] ‘ SVRIUSPTO-EXRF-5l27 ” DNlS:2733300 ‘ CSlD:2125830l50’ DURATION (mm-ss)'10-12
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`, U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7. 1995)
`Stavrianopoulos et al.
`Amendment To Applicants’ November 2, 2005
`Page 16 [Supplemental
`Amendment Under 37 C.F.R. §1.116]
`—- November 8, 2005]
`
`(7
`
`Claim 3235.
`
`(Previously Presented) The array of claim 3222, wherein said nucleic
`
`acids are DNA.
`
`Claim 3236.‘ (Previously Presented) The array of claim 3228, wherein said nucleic
`
`acids are DNA.
`
`(Previously Presented) The array of claim 3222, wherein said nucleic
`Claim 3237‘.
`acids comprise a gene sequence or pathogen sequence.
`
`(Previously Presented) The array of claim 3223, wherein one strand
`Claim 3238,
`of said double—stranded nucleic acids comprises a gene sequence or pathogen
`
`sequence.
`
`Claim 3239.
`
`(Canceled).
`
`(Previously Presented) The array of claim 3222, wherein said single-
`Claim 3240’.
`stranded nucleic acids are unlabeled.
`
`(Previously Presented) The array of claim
`Claim 3241.
`slucent.
`’ said non’-porous solid support is transparent or tran
`
`’
`
`’
`
`S 3222 or 3223, wherein
`
`(Previously Presented) The array of claim 3223, wherein said non-
`Claim 3242.
`radioactive signaling moiety is quantifiable in or from a fluid or solution, said
`quantity being proportional to the amount or quantity of said non-radioactive
`signaling moiety.
`
`Enz-7(Pl(C3)
`
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`Page 17 of 52
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`Stevrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7. 1995)
`Page 17 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.1’l6]
`—— November 8,; 2005]
`
`18
`
`(Previously Presented) The array of claim 3223, wherein said non-
`Claim 3243.
`porous solid support is transparent or translucent, and said non-radioactive
`signaling moiety is quantifiable in or from a fluid or solution or from said non-porous
`solid support, said quantity being proportional. to the amount or quantity of said
`
`non-radioactive signaling moiety.
`
`Claim 3244.
`
`(Previously Presented) The array of claim 3223, wherein said non-
`
`(Previously Presented) The array of claim 3223, wherein a non-
`Claim 3245.
`radioactive signal from said non-radioactive signaling moiety is quantifiable or
`
`detectable by photometric techniques.
`
`(Previously Presented) A non-porous glass or non-porous plastic solid
`Claim 3246.
`support comprising one or more aminels), hydroxyllsl or epoxidelsl thereon,
`wherein at least one single-stranded nucleic acid is fixed or immobilized in
`hybridizable form to said non-porous solid support via said one or more aminels),
`
`hydroxylls) or epoxidels).
`
`(Previously Presented) A non—porous glass or non—porous plastic solid
`Claim 3247.
`support Icomlprisingmone or more aminels), hydroxylls) or epoxidelsl thereon,
`‘
`rein at least one double-stranded nucleic acid is fixed or immobilized to said
`whe
`minelsli
`non—porous glass or non-porous plastic solid support via said one or more a
`_ hydroxyllsl or epoxidels), and wherein one nucleic acid strand of said double-
`stranded nucleic acid has covalently attached thereto either:
`(i)
`a nomradioactlve signaling moiety, or
`(ill
`a non—radioactive bridging moiety covalently or non-covalently
`attached to a non—radioactive signaling moiety.
`
`Enz-7 (PMC3)
`
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`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995).
`Page 18 [Supplemental AmendmentATo Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.1i6]
`-- November 8., 2005]
`
`(Previously Presented) The non—porous glass or non-porous plastic
`Claim 3248.
`,7, wherein said non—porous glass or non-porous
`solid support of claims 3246 or 324
`a, well or wells, a microtiter well or
`plastic solid support comprises a plate or plates,
`microtiter wells, a depression or depressions, a tube or tubes, or a cuvette or
`cuvettes.
`
`Claims 3249-3251 .
`
`(Canceled).
`
`(Previously Presented) The non-porous glass or non-porous plastic
`Claim 3252.
`solid support of claims 3246 or 3247, wherein said non—porous glass or non-porous
`plastic solid support has been treated with a surface treatment agent, a blocking
`agent, or both.
`
`(Previously Presented) The non-porous glassor non-DOFOUS Plastic
`Claim 3253.
`claim 3252, wherein said surface treatment agent compri
`solid support of
`$65 an
`on or
`amine providing compound, an epoxy glue or solution, an acid soiutl
`
`ammonium acetate.
`
`(Previously Presented) The non—porous glass or non-porous plastic
`Claim 3254.
`solid support of exam" 3252, wherein‘ said blocking agent comprises Denhardt‘s~
`
`(Previously Presented) The non-porous glass. or nonporous plastic
`Claim 3255.
`3246 or 3247, wherein said fixation or immobilization to
`solid support of claims
`said non-porous glass or non—porous plastic solid support is covalent.
`
`Enz-7lP)(C3)
`
`PAGEl9l52‘RCVDAT11!8l20054:50:40PM
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`Si
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`[E65 em an
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`(lard
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`r
`-
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`lime] SVR.USPl’0-EfXRFr5l2l DNlS.2i38300 CSlD:2125830150‘DURAT|0N(mm-ss):10-12
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`Page 19 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment'Under 37 C.F.Fl. §i.116l
`-- November 8, 2006]
`
`20
`
`(Previously Presented) The non~porous glass or non-porous plastic
`Cla