-
`-
`0Z'C£3I$9-W)ii0l.WHn0a09i0£89ZiZ'0lS3s90€fill8'SiiI0
`
`2 IHHXJE-Oidsn-HASxI3ii||l1ii5|iii90M91523]i'idZ0'£i'9900Zl9Z!9liiGi\Dii
`n
`'
`-
`-
`-
`-
`
`2
`
`ism
`
`save
`
`.
`
`figcgwso
`FAX CENTER
`MAY 2 5 2005
`
`Attorney Docket No.-: Enz-7(P)(C3)
`IN THE UNITED STATES PATENT AND TRADEMARK OFFIC ‘
`'71 F8 Application of:
`Jamie Stavrianopouios et ai.
`Serial No.:
`08/486,070
`Group Art Unit: 1631
`Filed:
`June 7, 1995
`SPE: Ardin H. Marschei,75h.D ”"""”""
`For:
`NUCLEIC ACIDSVFIXED OR IMMOBIIJZED TO NON-POROUS
`SOLID SUPPORT, AND SYSTEM, ARRAY AND NON-POROUS ~
`GLASS OR PLASTIC SOLID SUPPORT COMPRISING SUCH FIXED
`OR IMMOBILIZED NUCLEIC ACIDS
`.
`.
`Confirmation No.:
`v
`‘
`GERTIFICATE OF FACSIMILE TRANSMISSION
`
`jfij
`O W’
`2'5 3 V“
`m -S C?‘
`\
`I
`C5
`‘"4
`<<
`2 '55’ Q
`U3
`an
`
`Commiseioner for Patents-
`P.O. Box ‘I450
`Alexandria, VA 22313-1460 ’
`
`Sir:
`
`I hereby certify that the attached correspondence (total of 137 pages
`
`including this sheet) comprising:
`Amendment Under 37 C.F.R. §1.11 5
`(In Response To November 26, 2004 Office Action) [including Exhibits A, B & C1;
`
`and Request For Extension Of Time (3 Months)
`was sent to the United States Patent and Trademark Office by telefax to (703)
`
`872-9306.
`
`Date: May 25, 200E
`
`spectfuliy s mitted,
`
`
`onaid C. Fedus, Reg. No. 32,567
`Natalie Bogdanos, Reg. No. 51,480
`Attorneys for Appiicants
`Enzo Life Sciences, Inc.
`clo Enzo Biochem, Inc.
`527 Madison Avenue, 9th Floor
`New York, NY ‘I 0022-4304-
`Teiephone (212) 583-0100
`Facsimiie (212) 583-0150
`
`Enz—7(F'IIC3I
`
`i
`
`‘ii W9‘
`»
`
`0“
`
`Page 1 Of1OO
`
`1
`WJHOOIB OZN3
`
`wm=9
`
`SO0Z'§Z'AVW
`
`BD EXHIBIT 1013
`
`
`-
`0Z'C£3I$9-W)ii0l.WHn0a09i0£89ZiZ'0lS3s90€fill8'SiiI0
`
`2 IHHXJE-Oidsn-HASxI3ii||l1ii5|iii90M91523]i'idZ0'£i'9900Zl9Z!9liiGi\Dii
`n
`'
`-
`-
`-
`-
`
`2
`
`ism
`
`save
`
`.
`
`figcgwso
`FAX CENTER
`MAY 2 5 2005
`
`Attorney Docket No.-: Enz-7(P)(C3)
`IN THE UNITED STATES PATENT AND TRADEMARK OFFIC ‘
`'71 F8 Application of:
`Jamie Stavrianopouios et ai.
`Serial No.:
`08/486,070
`Group Art Unit: 1631
`Filed:
`June 7, 1995
`SPE: Ardin H. Marschei,75h.D ”"""”""
`For:
`NUCLEIC ACIDSVFIXED OR IMMOBIIJZED TO NON-POROUS
`SOLID SUPPORT, AND SYSTEM, ARRAY AND NON-POROUS ~
`GLASS OR PLASTIC SOLID SUPPORT COMPRISING SUCH FIXED
`OR IMMOBILIZED NUCLEIC ACIDS
`.
`.
`Confirmation No.:
`v
`‘
`GERTIFICATE OF FACSIMILE TRANSMISSION
`
`jfij
`O W’
`2'5 3 V“
`m -S C?‘
`\
`I
`C5
`‘"4
`<<
`2 '55’ Q
`U3
`an
`
`Commiseioner for Patents-
`P.O. Box ‘I450
`Alexandria, VA 22313-1460 ’
`
`Sir:
`
`I hereby certify that the attached correspondence (total of 137 pages
`
`including this sheet) comprising:
`Amendment Under 37 C.F.R. §1.11 5
`(In Response To November 26, 2004 Office Action) [including Exhibits A, B & C1;
`
`and Request For Extension Of Time (3 Months)
`was sent to the United States Patent and Trademark Office by telefax to (703)
`
`872-9306.
`
`Date: May 25, 200E
`
`spectfuliy s mitted,
`
`
`onaid C. Fedus, Reg. No. 32,567
`Natalie Bogdanos, Reg. No. 51,480
`Attorneys for Appiicants
`Enzo Life Sciences, Inc.
`clo Enzo Biochem, Inc.
`527 Madison Avenue, 9th Floor
`New York, NY ‘I 0022-4304-
`Teiephone (212) 583-0100
`Facsimiie (212) 583-0150
`
`Enz—7(F'IIC3I
`
`i
`
`‘ii W9‘
`»
`
`0“
`
`Page 1 Of1OO
`
`1
`WJHOOIB OZN3
`
`wm=9
`
`SO0Z'§Z'AVW
`
`BD EXHIBIT 1013
`
`
`
`0l‘l3£1i55'll1llli llolmlilll 2 09l0€8§ZlZ5CllSD x 9fllI6Zl85Slil0 2 0il':iilX:l3'0l.dSil3iii\S slalllll. lll5lW30 W9l5?3i llld l0'Ei7'9 §ilDZi9Zl9 iii (JADE! Z8ll9 Bfliid
`
`IN THE UNlTED STATES PATENT AND TRADEMARK OFFICE
`
`
`
`Applicantlslz
`Serial No.;
`.
`Filed:
`For:
`
`Stavrianopoulos, er al.
`O8/486,070
`June 7, 1995
`‘NUCLEIC ACIDS FlXED on IMMOBILIZED
`
`,
`)
`i
`i
`i
`
`)
`To NON-POROUS soup supponr. AND
`SYSTEM. ARRAY AND NON~POROlJS GLASS )
`on PLASTIC sour) SUPPORT compmsms
`)
`sucu FIXED onuvnvioeruzso NUC:LEiC ACIDS l
`
`RECEIVED
`“mm” “X CENTER
`MAY .2 5 2005
`Group Art Unit: 1631
`SPE: Ardin H. Marschel, Ph.D.
`“
`
`A
`
`E
`
`527 Madison Avenue, 9"‘ Floor
`New York, NY 10022-4304-
`
`May 25, 2005
`
`VIA FILED B Y FA CSIMILE TRANSMISSION
`TO: I703)‘ 872-9306‘
`
`Mail Stop -- Amendment
`Commissioner for Patents
`P.O. Box 1450
`Alexandria, VA 22313-1450
`
`AMENDMENT UNDER 37 C.F.R. §'l .115
`(IN RESPONSE TO THE NOVEMBER 26. 2004 OFFICE ACTION)
`
`Dear Sirs:
`
`This paper (Amendment Under 37 C.F.R. §1.1‘l5) is responsive to the
`November 26, 2004 Office Action mailed in connection with the above-identified
`application. A response to the November 28, 2004 Office Action was initially due
`by February 26, 2004. This paper is accompanied by a Request For Extension Of
`Time (Three Months), and authorization for the fee therefor. Upon the expected
`granting of Applicants‘ extension request, the response deadline will be extended
`to May 26, 2005. Accordingly, this paper is being timely filed.
`
`Enz-7(PllC3l
`
`9'3
`
`Pagezofloo
`
`lZi7S'ON
`
`wmoieozwa
`
`.
`
`lNd§l2»9
`
`saoztmvw
`
`
`
`0Z'€€3(55‘WU1) Noilifiiflfl x O9i0€8§ZiZ50iS3 2 9ii€6Zl85SiNO x 0il':iiiX:i3'0ldSfl3iii\S x[3WL1 1li5lW’«0 W593] Uid 30181739 90011935 N GAD}! .icuz 39W
`Stavrianopouios et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`-
`
`Page 2 [Amendment Under 37 C.F.R. §1.1i5 (in Response To The
`November 26, 2004 Office Action) —— May 25, 2005]
`
`
`
`CETiFlCATE OF TRANSMISSION
`
`I hereby cartily that this paper (Amendment Under 37
`C.F.R. §1.115 (in Response To The November 26, 2004
`Office Action) (along with any documents referred to an
`attached or enclosed) is being facsimile transmitted to
`the United States" Patent and Trademm (Fax No. 703-
`872-9306) on May 25.
`
`
`
`
`
`
`
`‘
`
`
`
`
`
`My 25' 2023';
`Date
`
`Honaid
`..F8dU8
`
`Reg.No. 32,567 /
`
`
`
`
`Amendments to the Claims are reflected in the listing of ciaims which begins on
`
`page 3 of this paper.
`
`Remarks begin on page 45 of this paper.
`
`' Enz-7(Pi((33i
`
`L
`‘d
`M179 ‘ON
`Page?) OHOO
`
`w3H313
`O
`
`OZN3
`
`:
`i’id§’V 9
`
`-
`V
`9006 El AVW
`
`
`
`s llll'JllX:l3'0l.dSlli2lllS 1 Will lllfill/ilill l1J3l5li3l llld Zlli€i'39 9GOZl9Zl9 H (MD?! : [EH8 Sillld
`0Z'€€1l99‘l'1lllilll)l.lll'h‘lllJxll9i(liI89llZ3CllSl'lx9D96Zl3?Sllll0
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 3 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) —— May 25, 2005]
`
` : This listing of the claims below will replace all prior
`
`versions and listing of claims in this application.
`
`Listing of Claims:
`
`Claims 1-3143 (Canceled).
`
`Claim 3144.
`
`(Currently Amended) A non~porous solid support comprising one or .
`
`more aminelsl. hydroxyllsl or egoxidelsl thereon. wherein at least one single-
`
`stranded nucleic acid is
`
`fixed or immobilized thereto in
`
`hybridizable form to said non-porous solid suggort via" said one or more amlnelsl,
`hydrogllsl or egoxidelsl.
`c
`o u
`s
`
`
`
`
`
`
`
`
`
`u 1 u — I ~ ‘ I 0 - : In i 1‘Q
`
`
`
`
`
`
`
`
`
`
`Claim 3145.
`
`(Currently Amended) A non—porous solid support comprising one or
`
`more aminels), hydroxyllsl or epoxidelsl thereon‘ wherein at least one double»
`
`stranded nucleic acid i_:_3_ fixed or immobilized theeetee to said non—Qorous solid
`
`suggort via said one or more aminelsl, hydroxylls) or epoxidelsl, and wherein at-.
`
`least one nucleic acid strand of said at least one double-stranded nucleic acid has
`
`covalently attached thereto either:
`
`_
`
`ill
`
`(ill
`
`a nomradioactive signaling moiety, or
`
`a non-radioactive bridging moiety covalently or non-covalently
`
`attached to a non—radioactive signaling moiety.
`
`
`
`Enz—7(PllC3)
`
`8'd
`lZi§‘ON
`Pagemfloo
`
`wauooiaozwa
`
`-
`;
`wd9l>9
`
`-
`.
`900% SZAVW
`
`
`
`0l‘£€3(35'“1"1)N0llVili)0 s09)0€89Zl«Z5GlS3x 90€6Zl3§Slli0 u UIi’:iiiX:i3'OldSi)3iii‘lS s [will IUWPG M33593] Nd 3059139 900ZI9ll9 W 0:108 u [W5 39\l'd
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 4 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3146.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3144,, er 3145. 3287, 3324 or 3325, wherein said non-porous solid support
`
`comprises glass or plastic.
`
`Claim 3147.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3144‘ or 3145, 3287, 3324 or 3325, wherein said non—porous solid support
`
`comprises a plate or plates, a well or wells, a microtiter well or microtiter wells, a
`.__...e..._...
`depression or depressions, a tube or tubes, a cuvette or cuvettes, or a bead or
`beads .
`
`Claim 3148-3149.
`
`(Canceled).
`
`Claim 3160.‘ (Currently Amended) The non—porous solid support of claim 3287 or
`3325
`comprising es-mpsiseone
`or more amines .
`
`Claim 3151.
`
`(Currently Amended) The non—porous solid support of elaim gl_g_i_m_s
`
`3144; or 3145. 3287, 3324 or 3325, wherein said non-porous solid support has
`been treated with a surface treatment agent, a blocking agent, or both.
`
`(Previously Presented) The non-porous solid support of claim 3151,
`Claim 3152.
`wherein said surface treatment agent comprises an amine providing compound, an
`
`epoxy glue or soiution, an acid solution, or ammonium acetate.
`
`(Previously Presented) The non-porous solid support of claim 3151.
`Claim 31 53.
`wherein said blocking agent comprises Denhardt‘s solution.
`
`Enz-7(P)(C3)
`
`6
`
`'d
`
`Pages Ofloo
`
`M179 'Oll
`
`W3H30l8 OZN3
`
`:
`
`M917 9
`
`'
`
`-
`
`9005 95 /\VW
`
`
`
`llZ'lllI1i35‘WWlNOl1ii‘2i(l0 x ll9l»lll.'89ZiZ5GlS0 x 9U96ZZ85Slli0 x lJll':lilX:l3‘0ldSll5llIlS « l3Wl.l il-l5ilKl?Cl UJ3i593l l'ld Z01€i39 9l1flZl‘:‘li9 1)! (M03 x lilllll Efiilld
`Stavrianopoulos et al., U.S. Pat. Appl. Ser; No. 08/486,070 (Filed June 7, 1995)
`
`Page 5 (Amendment Under 37 C.F.R. §1.1i5 (in Response To The
`November 26, 2004 Office Action) —- May 25, 2005]
`
`Claim 3154.
`
`(Currently Amended) The non—por0us solid support of claim claims '
`
`3144) 9.: 3145 3287 3324 or 3325, wherein said
`
`fixation or
`
`immobilization to said non)-porous solid support is covalent.
`
`Claim 3155.
`(Currently Amended) The non—porous solid support of claim claims
`3144‘ er 3145, 3287, 3324 or 3325, wherein said
`fixation or
`
`immobilization to said non—porous solid support is non—covaIent.
`
`Claim 3156.. (Canceled).
`
`(Currently Amended) The non-porous solid support of eiaim claims
`Claim 3157.
`
`3145 3287 or 3325, wherein one strand of said at least one double-stranded
`
`nucleic acid is indireetly fixed or immobilized to said non-porous solid support by
`and the other
`neeieieaeid strand of said at least one double~stranded nucleic acid is hybridized to
`
`. said one strand.
`
`Claim 3158.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3i 44 or 3324, wherein said nucleic acid eempr-ises is DNA£r—RNA.
`
`Claim 3159.
`
`(Currently Amended) The non-porous solid support of eiaim claims
`
`3145: 3287 or 3325, wherein said nucleic acid eemeeises i_s_ DNA .
`
`Claim 3160.
`
`(Previously Presented) The non-porous solid support of claim 3144,
`
`wherein said nucleic acid comprises a nucleic acid sequence complementary to a
`
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`Enz-7(P)lC3)
`
`oi
`‘a
`lz:79'oN
`Pageéofloo
`
`WHO’
`3
`018 OZNB
`
`:
`win 9
`
`-_
`-
`9005 $5 /WW
`
`
`
`llZ'l2€1(35'lllUll NOILVHHO x 09l0€89ZlZ30lS3 s90lI6ZZ83SlllClsllll':lllX$3'0ldSliRll'lSulilllllllllfillfililll1l3l3li3]l‘ldZ01£l399ll0Zl9Zl9llf GAO?! llllll Elfllld
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`8
`
`Page 6 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The non-porous solid support of elaim claims
`Claim 3161.
`3145 or 3287, wherein one strand of said at least one double-stranded nucleic acid
`
`comprises a nucleic acid sequence of interest sought to be identified, quantified or
`
`sequenced.
`
`Claim 3162.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3144 or 3324, wherein said slngle—stranded nucleic acid is unlabeled.
`
`Claim 3163.
`(Currently Amended) The non-porous solid support of elaim claims
`3144‘ er 3145, 3287. 3324 or 3325, wherein said non-porous solid support is
`
`transparent or translucent.
`
`(Currently Amended) The non-porous solid support of el-aim c_lza_ifl1_s
`Claim 3164.
`3145 or 3287, wherein said
`non'—[ggioactive
`signaling moiety is quantifiable in or from a fluid or solution, said quantity being
`proportional to the amount or quantity of said label-er—lalaels non—radioactive
`
`'
`
`signaling moiety.
`
`‘
`(Currently Amended) The non~porous solid support of e-laim <:l_ai_r_n_'s_
`Claim 3165.
`3145 or 3287, wherein said non-porous solid support is transparent or translucent,
`and said
`non-radioactive si nalin moiet
`is
`quantifiable in or from a fluid or solution or in-er-through f_rg_rr_1 said non-porous solid
`support, said quantity being proportional to the amount or quantity of said laleel—er
`labels non-radioactive signaling moiety.
`
`Enz-7(P)(C3)
`
`ii
`
`‘a
`
`Pagwoflofl
`
`lZl79'0ll
`
`I
`
`A
`
`ll3H30l8OZN3
`
`.
`
`.
`
`wa9t9
`
`.
`
`.
`
`QOOZQZAVIN
`
`
`
`'
`'
`:
`ll0l1V3l'lG : ll§l0E89ZlZ1UlS0 x 90E6ZZ85SlllU xUlH2lX:l3‘0.ldSll?illlS xlillllllll5l|r(9€ll1l3l5‘33]l‘ldZ0?£l19900Zl9Zl9.lll HASH 1.9HZl 39Vd
`0Z'lI€3l59'WWl
`Stavrianopoulos et al.. U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 7 [Amendment Under 37 C.F.R. §1.l15 (in Response To The
`November 26. 2004 Office Action) -- May 25, 20051
`
`Claim 3166.
`
`(Currently Amended) The non-porous solid support of elaim claims
`
`3145, 3287 or 3325, wherein said
`
`non-radioactive
`
`signaling moiety comprises a chromagen or a chromagenic compound
`
`
`
`Claim 3167.
`
`(Currently Amended) The non—porous solid support of claim claims
`
`3145, 3287 or 3325, wherein a non-radioactive signal from said nee-r-aelieastive
`ehemieal-laiaei non—radioactive signaling moiety is quantifiable or detectable by
`
`photometric techniques
`
`(Currently Amended) The non—porous solid support of'eleim claims
`Claim 3168.
`3144 or 3324, comprising more than one single—stranded nucleic acid.
`
`Claim 3169.
`(Currently Amended) The non-porous solid support of elaim _gl_a_i_r;s
`3145, 3287 or 3325, comprising more than one double—stranded nucleic acid.
`
`Claims 3170 - 3171 (Canceled).
`
`(Currently Amended) A system comprising a non-porous solid
`Claim 3172.
`support which com rises one or more amine s
`h Vdrox l s or e oxide s thereon
`wherein and at least one singlestranded nucleic acid is
`fixed or
`
`Enz-7(Pl(C3)
`
`at
`
`‘a
`
`Pagegofmo
`
`lZl7§'Oll
`
`l*l3H30l8 ozma
`
`.
`
`waltz-9
`
`.
`
`.
`
`900% 9a AVW
`
`
`
`DZ'££3(55’Wl1l)ll0llVllllClxU9lflli89ZlZ3lllS3 2 9.0E6Zl8iSlNCl uUl)':lllX:lEl'OldSll1llIlS xiaulll lll5lMPG lU3l3P3l llld 3038739 900Zl9ll9 ll! Clllilll
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 8 {Amendment Under 37 C.F.R. §1.l15 (In Response To The
`November 26, 2004- Office Action) -— May 25, 2005]
`
`llilllll Bflild
`
`(Currently Amended) A system comprising a non—porous solid
`Claim 3173.
`support which comgrises one or more aminels), hydroxyl(s) or egoxide(s) thereon,
`wherein and at least one double-stranded nucleic acid is fixed or immobilized
`ti-‘mete-, to said non-porous solid support via said one or more amlnelsl, hydroxylls)
`or epoxidels), and wherein eeleast one nucleic acid strand of said double-stranded
`nucleic acid has covalently attached thereto either:
`
`(i)
`(ii)
`
`a nomradioactive signaling moiety, or
`a non-radioactive bridging moiety covalently or non-covalently
`attached to a non-radioactive signaling moiety.
`
`
`
`(Currently Amended) The system of eleim claims 3172‘ er 3173,
`Claim 3174.
`3279, 3280, 3281 or 3282, wherein said non—porous solid support comprises glass
`
`or plastic.
`
`(Currently Amended) The system of eleim claims 3172,_ 9: 8173,
`Claim 3175.
`3279, 3280, 3281 or 3282, wherein said non—porous solid support comprises a
`plate or plates, a well or wells, a microtiter well or microtiter wells, a de ression or
`depressions, a tube or tubes, a cuvette or cuvettes, or a bead or beads
`I
`I
`'
`V
`
`Claims 3176-3178.
`
`(Canceled).
`
`Enz—7(PllC3)
`21
`‘
`-
`Page9“Of1‘05g9 ON
`
`t
`
`A
`
`l’l3H3OI8 OZN3
`
`'
`
`.
`Wdli-9
`
`SO0Z'SZ'AvlN
`
`
`
`I3Z'£i33i55‘l1ll1liN0ll‘iiinGs09i0£8§ZlZ3GlS0:90€6Zl83Slli0s0ll'£ilX:l3'0ldSil53flSsi3Ulll
`iufillllva (M15931 llld Z05l3i’9§l)0Zl9Zl9 1))’ am mm asrd
`Stavrianopoulos et aI., u.s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`'
`
`Page 9 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3179.
`(Currently Amended) The system of elaim claims 3172, eta 3173,
`3279, 3280, 3281 or 3282, wherein said non-porous solid support has been
`treated with a surface treatment agent, a blocking agent, or both.
`
`(Previously Presented) The system of claim 3179, wherein-said
`Claim 3180.
`surface treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3181.
`
`(Previously Presented) The system of claim 3179, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Claim 3182.
`
`(Currently Amended) The system of elaim claims 3172, on 3173,
`
`
`
`279 3280 3281 or 3282 wherein said fixation or
`
`immobilization to said non-porous solid support is covalent.
`
`Claim 3183.
`
`(Currently Amended) The system of eiaim claims 3172, e): 3173,
`
`fixation or
`3279, 3280, 3281 or 3282, wherein said
`immobilization to said non-porous solid support is non-covalent.
`
`Claim 3184.
`
`(Canceled).
`
`(Currently Amended) The system of eleim claims 3173, 3281 or
`Claim 3185.
`3282, wherein said one strand of said doublestranded nucleic acid is indirectly
`fixed or immobilized to said non—porous solid support
` and the other nucleic acid strand of said
`double-stranded nucleic acid is hybridized to said one strand.
`
`Enz—7(Pl(C3)
`
`)1
`"ii W9‘
`PagemOf1000“
`
`l*lElH0Ol8OZNEl
`
`.
`watt-9
`
`‘
`sooc'9z'ivw
`
`
`
`llZ'E93(55‘wWiN0l1VHllG : 09l0989ZlZ10lS0 :9lll.‘6ZZ35Sllll1 i0lHllX:l3'0.l.dSll5llr\S aiawll lllfill-‘PG W593] llld Z03£i'39 9DOZl9Zl9 1V 0/133 [W91 39Vd
`Stavrianopoulcs et al., U.S. Pet. App). Ser. No. 08/486,070 (Filed June 7, 1995)
`t
`
`Page 10 [Amendment Under 37 C.F.Fl. §1.115 (In Response To The
`November 26, 2004 Office Action) —— May 25, 2005]
`
`Claim 3186.
`
`(Currently Amended) The system of eleim claims 3172 or 3280,
`
`wherein said nucleic acid Q eeneerises-DNA—er—RNA.
`
`(Currently Amended) The system of elaim claims 3173, 3281 or
`Claim 3187.
`3282, wherein said nucleic acid is DNA .
`'
`
`(Previously Presented) The system of claim 3172, wherein said
`Clalm 3188.
`nucleic acid comprises a nucleic acid sequence complementary to a nucleic acid
`sequence of interest sought to be identified, quantified or sequenced.
`
`(Currently Amended) The system of elaim claims 3173 or 3288,
`Claim 3189.
`wherein one strand of said at least one double-stranded nucleic acid comprises a
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`* Claim 3190.
`
`(Previously Presented) The system of claim 3172, wherein said
`
`single—stranded nucleic acid is unlabeled.
`
`(Currently Amended) The system of claim claims 3172, as 3173,
`Claim 3191 .
`3279, 3280, 3281 or 3282, wherein said non—porous solid support is transparent
`
`or translucent.
`
`(Currently Amended) The system of eleiee claims 3173 or 3288,
`Claim 3192.
`non-radioactive signaling moiety is
`wherein said
`quantifiable in or from a fluid or solution, said quantity being proportional to the
`amount or quantity of said laeel—er—leleels non—radioactive signaling moiety.
`
`Enz-7{P}(C3)
`
`'9
`‘d
`91
`Page1]Ofl]5gg0ll
`
`-
`Vl3H30l8OZN:l
`
`.
`wait-9
`
`9001 '96 "WW
`
`
`
`GZ'lI€1l33'WlN0llllllflG:09lll£39ZlZ1lllS3a9096Zl81Slll0z0ll':lllX:l3'0ldSll3HIlSshllllllllillllillalU9lS93]llld201E159900Zl9Zl9l‘l'Oll0lixZlIll9l 3i>l1d
`Stavrianopoulos at al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 11 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The system of elaim claims 3173 or 3288,
`Claim 3193.
`wherein said non-porous solid support is transparent or translucent, and said nea-
`non-radioactive signaling moiety is quantifiable in or from
`a fluid or solution or in—er——%hreegh from said non—porous solid support, said quantity
`being proportional to the amount or quantity of said lalsel~er—laleels non-radioactive
`
`signaling moiety.
`
`(Currently Amended) The system of elalm claims 3173, 3231 or
`Claim 3194.
`3282, wherein said
`non-radioactive signaling moiety
`comprises a chromagen or a chromagenic compound $e
`
`
`
`(Currently Amended) The system of elaim claims 3173, 3281 or
`Claim 3195.
`3282, wherein a nonwadioactive signal‘ from said
`non-radioactive. signaling moiety is quantifiable or detectable by photometric
` In I
`a
`
`.
`
`‘
`
`‘technique ,
`.
`
`.
`
`.
`
`Claim 3196.
`
`(Previously Presented) The system of claim 3172, comprising more
`
`than one single-stranded nucleic acid.
`
`Claim 3197.
`
`(Currently Amended) The system of
`
`claims 3173, 3281 or
`
`3282, comprising more than one double-stranded nucleic acid.
`
`. Enz-7(P)(C3)
`
`911;
`
`Pagelzofmo N
`
`151790
`
`w3Hooiaozii3
`
`.
`
`wast-9
`
`900Z'9Z‘A‘v’lll
`
`
`
`oz-ccilss-ww) llolmlnu . 09ll)£89ZlZ1lJlSO I 90l36il8iSlllll iwi-aaxasoldsntais uliilllllillfilllllilll1l9l59El)l‘ldZ01€)1990flZl9Zl9lVl]il32l mm 39lld
`Stavrianopoulos et al., u.s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`'1
`
`Page 12 [Amendment Under 37 C.F.R. §1.1 15 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) An array ‘comprising various single-stranded
`Claim 3198.
`fixed or immobilized in hybridizable form to a non-
`nucleic acids
`porous solid supponrwmm
`
`
`(Currently Amended) An array comprising various doubie—stranded
`Claim 3199.
`fixed or immobilized to a non-porous solid
`nucleic acids
`support, wherein at least one nucleic acid strand of said various double-stranded
`nucleic acids comprises at least one non-radioactive ehemie~sl—laeel signaling moiety
`
`
`
`(Currently Amended) The array of elaim claims 3198L st: 3199,
`Claim 3200.
`3300, 3301, 3326 or 33_2_'_/4 wherein said non—porous solid support comprises glass
`
`or plastic.
`
`(Currently Amended) The array of elaim claims 3198‘ er 3199,
`Claim 3201.
`3300, 3301., 3326 or 3327. wherein said non—porous solid support comprises a
`plate or glates, a well or walls, a microtiter well or microtiter wells, §_d§Q[e_§§i_gr;_Q[
`depressions, a tube or tubes, a cuvette or cuvettes, or a bead or beads
`,
`,
`,
`,
`s~ar—beads.
`
`Claim 3202 -3203.
`
`(Canceled).
`
`Enz-7(F)lC3)
`
`ll
`'
`--
`d W9 0'4
`y Page 13 0f100
`
`y
`
`wamoia OZN3
`
`.
`rider-9
`
`sooztmvw
`
`
`
`0Z'EE3i55'W) Nolmlflfl s (l9l0889lll1illS0 x 90E6Z281SlllCl r 0ll'JilX:lEi'0ldSll3il/lS 2 Will lU5!l«iPO 1193993] llld E03719 900Zl9Zl9 1V G/(OH :’£Eil8l 39Vd
`Stavrianopoulos at al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 13 [Amendment Under 37 C.F.R. §a1.1'l5 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The array of claims 3198, 3199, 3300, 3301 ,
`Claim 3204.
`3326 3322 1..
`saga,
`'
`.
`.l
`.
`.
`1..
`1.
`.
`.
`
`comprising one or more amines .
`
`(Currently Amended) The array of elaimclaims 3198,, as 3199,
`Claim 3205.
`3300, 3301, 3326 or 3327, wherein said non—porous solid support has been
`treated with a surface treatment agent, a blocking agent, or both.
`
`(Previously Presented) The array of claim 3205, wherein said surface
`Claim 3206.
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3207.
`
`(Previously.Presented) The array of claim 3205, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`(Currently Amended) The array of elaire claims 3198, as 3199,
`Claim 3208‘.
`3300, 3301, 3826 or 3327, wherein said
`fixation or
`immobilization to said non—porous solid support is covalent.
`
`(Currently Amended) The array of ,elaim claims 34198, e»: 3199,
`Claim 3209.
`3300, 3301, 3326 or 3327, wherein said
`fixation or
`immobilization to said non-porous solid support is nomcovaient.
`
`Claim 3210.
`
`(Canceled).
`
`Enz—7lPNC3)
`
`8!
`
`'d
`
`Page 14 01,100
`
`lZi7S '0N
`
`l’l3H30l8 OZN3
`
`.
`
`l*ld8i7'9
`
`9005 '95 '/WW
`
`
`
`0Z'l)l:i(55'lWl)llOl1VilnGxll9l0l389ZlZ?(ilS0a90£6ZZ81SllllJ:0ll':lilXd3-OldsllillllsslallllllllfiilliliflUJ9i5l?El] llldZ01£i199llllZl9Zl9lllllllliilxllllllilRiliid
`Stavrianopculos et al., U.S, Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 14 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`‘
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) The array of claim claims 3199 or 3301,
`Claim 3211.
`wherein ene-strand the first strands of said double~stranded nucleic acids can
`seeeeneeeie are
`fixed or immobilized to said non-porous solid support by
`and the other nucleic
`acid strands of said double-stranded nucleic acids are hybridized to said first
`
`strands.
`
`Claim 3212.
`
`(Currently Amended) The array of elaim claims 3198 or 3300,
`
`wherein said nucleic acids is__eemerise—DNA—er—-RNA.
`
`(Currently Amended) The array of eiaim claims 3199 or 3301,
`Claim 3213.
`wherein said nucleic acids §mmedseDNA .
`
`(Currently Amended) The array of claim claims 3198 or 3289,
`Claim 3214.
`(wherein said nucleic acids comprise nucleic acid sequences complementary to
`nucleic acid sequences of interest sought to be identified. quantified or sequenced.
`
`(Currently Amended) The array of eiaim claims 3199 or 3290,
`Claim 3215.
`wherein one strand of said double~stranded nucleic acids comprises a nucleic acid
`sequence of interest sought to be identified, quantified or sequenced.
`
`(Currently Amended) The array of claim claims 3198 or 3300,
`Claim 3216.
`wherein said single-stranded nucleic acids are unlabeled.
`
`(Currently Amended) The array of eleim claims 3198Ler 3199,
`Claim 3217.
`3300, 3301 , 3326 or 3327, wherein said non-porous solid support is transparent .
`
`or translucent.
`
`Enz-7(P)(C3)
`
`61
`
`‘d
`
`Page15Of]g00N
`
`lZ'€«‘
`
`1
`
`.
`
`W3HI)0l8OZN3
`
`..
`
`waew SO0E'§Z'/will
`
`
`
`ii0il‘i8lll] r ll9l0lZ89ZlZ30|83 iillllifillliisliifl :0ll'3iiX:l3'0l.dSll1HAS ai3W|l iil5iW3('l UJ3l9P3i llld Z03lIl19900Zl9Zl9lV Cllliiii 19Hill
`0Z'9€7i55'WWi
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`x
`
`am“
`
`Page 15 [Amendment Under 37 C.F.R. §l .1 15 (In Response To The
`November» 26. 2004 Office Action) -- May 25, 20051
`
`(Currently Amended) The array of elaim claims 3199 or 3290,_
`Claim 3218.
`wherein said non-radioactivee signaling moiety is quantifiable in or
`from a fluid or solution, said quantity being proportional to the amount or quantity
`
`of said label or labels.
`
`(Currently Amended) The array of eiaim gl§i;n_s 3199 or 3290,
`Claim 3219.
`wherein said non—porous solid support is transparent or translucent, and said non-
`
`. radioactive ehenai<:+il4abel signaling moiety is quantifiable in or from a fluid or
`
`solution or ir=H3i=-tlareugh mm said non~porous solid support, said quantity being
`
`proportional to the amount or quantity of said eB non-radioactive
`
`signaling moiety.
`
`(Currently Amended) The array of elaim claims 3199. 3301, 3326 or
`Claim 3220.
`3327, wherein said non—radioactivem signaling moiety comprises a
`I
`
`chromagen or achromagenic compound,
`
`
`
`-s--'.-:.-: .
`
`
`-:..
`
`‘e
`
`'-
`
`
`Claim 3221 .
`
`(Currently Amended) The array of eleim claims 3199, 3301 , 3326 or
`
`3327, wherein a non-radioactive signal from said non-radioactive me
`
`signaling moiety is quantifiable or detectable by photometric techniques;
`
`
`
`Enz-7(P)iC3i
`
`OZ
`
`‘
`
`Page 156 Ofilagg 0“
`
`'
`
`.
`
`.
`
`W3HO0l8 OZN3
`
`wa8r=9
`
`§OOZ'SZ'AVll\l
`
`
`
`0Z'E£3(35‘Wl llolmlllfl v 09l0E89ZlZ30lS0 2 9UE6Zl83SlN0 x 0ll'i2lXJ3'01dSllill}lS 1: [W1 lllfillriifl 131915933 Nd Z0'€l'9 §UOZl9Zl§ lll GAGE llillll §9Vd
`
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`x
`
`Page 16 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) An array comprising various single-stranded
`Claim 3222.
`fixed or immobilized in hybridizable form to a non—
`nucleic acids
`porous solid support having wells or depressions
`
`
`
`Claim 3223. "(Currently Amended) An array comprising various double-stranded
`nucleic acids
`fixed or immobilized to a non—porous solid support
`having wells or depressions, wherein at least one nucleic acid strand of each of
`said various double-stranded nucleic acids comprises at least one non-radioactive
`% signaling moiety which is quantifiable or detectable
`
` --- ..-.--‘..e---=:..
`
`-
`
`(Currently Amended) The array of claim claims 3222 or 3223,.
`Claim 3224.
`wherein said non—porous solid support comprises glass or plastic.
`
`Claim 3225 + 3226.
`
`(Canceled).
`
`Claim 3227.
`
`(Currently Amended) ihe array of claims 3222 or 3223 ,
`wherein said non—porous solid
`support comprises one or more amines %.
`
`Enz—7(P)(C3l
`
`ll
`
`“a
`
`Page17Ofl0090N
`
`in '
`
`_
`
`.
`
`W3H30l80ZN3
`
`.
`
`wast-9
`
`SO0Z'SZ’AVW
`
`
`
`0Z'€€1l55'WUll N0llVllll0 i09llll$89ZlZi(llS0 x 9fl95Zl85Sll((J i 01l':l2lX:l3'0ldS(l3li\S ¥ Will lll5ll:)Pi] Wills?!) Nd Z05£)19900Zl§Zl9 lV(lll3H lllllll 39lld
`Stavrianopoulos et)al., U.S. Pat. Appl. Ser. No. O8l486,070'(Filed June 7, 1995)
`
`Page 17 [Amendment Under 37 ’C.F.R. §1.‘l 15 (in Response To The
`November 26. 2004 Office Action) -- May 25, 2005]
`
`Claim 3228.
`
`(Currently Amended) The array of eleim claims 3222 or 3223,
`
`wherein said non—porous solid support has been treated with a surface treatment
`
`agent, a blocking agent, or both.
`
`Claim 3229.
`
`(Previously Presented) The array of claim 3228, wherein said surface
`
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3230.
`
`(Previously Presented) The array of claim 3228, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Claim 3231.
`
`(Currently Amended) The array of claim claims 3222 or 3223,
`
`wherein said
`
`fixation or immobilization to said non-porous solid
`
`support is covalent.
`
`Claim 3232.
`
`(Currently Amended) The array of elaim claims 3222 or 3223.
`
`wherein said
`
`fixation or immobilization to said non~porous solid
`
`support is non—covalent.
`
`Claim 3233.
`
`(Canceled).
`
`Claim 3234.
`
`(Currently Amended) The array of claim 3228, wherein esestrand
`
`the first strands of said double-—stranded nucleic acids
`
`a_r_e_ fixed or
`
`immobilized to said non-porous solid support
`
` and the other nucleic acid strands of said double
`
`stranded nucleic acids are hybridized to said first strands.
`
`Enz-7(P)(C3)
`
`iaizs‘oii
`"i
`ii
`Pagelgomo
`
`~
`iiaiiooii 0Zll3
`
`.
`.
`;
`wiei9 soon at AVW
`
`
`
`oz-cszlss-wwl llollliiinli2l19i0l389ZlZ3illS0i:90lI6Zl81S|ilG:0ll':iilX:l&0ldSni?lllS»i3l1lll iufillea iuaisigj mzo:cy:g9ooz)9g;;1y W mmw
`
`Stavrianopoulos et al., u.s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`‘
`
`Page 18 [Amendment Under 37 C.F.R. ‘ii .116 (in Response To The
`November 26, 2004 Office Action) -- May 25, 20051
`
`Claim 3235.
`
`(Currently Amended) The array of claim 3222, wherein said nucleic
`
`acids are DNA.
`
`Claim 3236.
`
`(Currently Amended) The array of claim 3223, wherein said nucleic
`
`acids ge_D&\_._
`
`(Currently Amended) The array of claim. 3222, wherein said nucleic _
`Claim 3237.
`acids comprise a gene seguence or pathogen seguencemQ
`I
`I~
`.‘
`>
`,
`F’:
`-F
`"
`
` .
`
`(Currently Amended) The array of claim 3223, wherein one strand of.
`Claim 3238.
`said doublestranded nucleic acids comprises a gene seguence or Qathogen
`seguence
`‘
`3
`._ " V,
`"
`
`
`
`
` .
`
`Claim 3239.
`
`(Canceled).
`
`Claim 3240.
`
`(Previously Presented) The array of claim 3222, wherein said single
`
`stranded nucleic acids are unlabeled.
`
`(Currently Amended) The array of eiaim claims 3222 or 3223,
`Claim 3241.
`wherein said non—porous solid support is transparent or translucent.‘
`
`(Currently Amended) The array of claim 3223, wherein said non-
`Claim 3242.
`radioactive% signaling moiety is quantifiable in or from a fluid or
`solution, said quantity being proportional to the amount or quantity of said iai9el—er
`
`iaeels non-radioactive signaling moiety.
`
`Enz-7iP)(C3)
`
`in '
`‘a
`er
`Pagelgomgoi
`
`V
`
`lNilHOOl8 OZN3
`
`.
`wan-9
`
`.
`sooz SE’/\VW
`
`
`
`0Z'E£3(59'Wll1i NOILVHHG 2 fl9l0€89ZiZ1UlS0 :. 9lJE6ZZ33SliiG : 0ll':iElX:i3'0ldSfl5il/lS : iaulll ilifillliifl lU3i5l?3i iild 30591739 90031909 iii GAD! 8 [SIM HOW
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 19 [Amendment Under 37 C.F.R. §1.115 (in Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3243.
`
`(Currently Amended) The array of claim 3223, wherein said non-
`
`porous solid support is transparent or translucent, and said non-radioactive
`eheraic—al—labei signaling moiety is quantifiable in or from a fluid or solution or in—er
`through from said non-porous solid support, said quantity being proportional to the
`amount or quantity of said melabeis non-radioactive signaling moiety.
`
`Claim 3244.
`
`(Currently Amended) The array of claim 3223, wherein said non-
`
`radioactive eiaeea-ieai—iaeei signaling moiety comprises a chromagen or a
`
`
`
`Claim 3245.
`(Currently Amended) The array of claim 3223, wherein a non—
`radioactive signal from said non-radioactive eheeaie~al-laieel si nalin moie
`is
`quantifiable or detectable by photometric techniques
`..
`;--.4
`no
`
`
`
`
`
`Claim 3246.
`
`(Currently Amended) A non-porous glass or non-porous plastic solid
`
`support comprising one or more amineisi, hydroxyusi or epoxide(s) thereon,
`wherein at least one single-stranded nucleic acid § ¢maw fixed or
`
`immobilized thereto in hybridizable form to said non-porous solid support via said
`one or more arnine(e), h\__{dr0xyl(s) or egoxlde(s)
`¥.
`.|..i..}§.
`‘
`;
`I1.‘
`in‘
`I
`I
`l
`o
`luv
`‘
`
`'
`
`i
`
`E:
`
`I.
`i
`
`I
`
`l
`
`F:
`
`I
`
`A
`
`.3
`
`llv
`
`K:
`
`n-
`
`Enz-7(P)(C3)
`
`it
`
`"a ms‘
`
`Pagezoomo 0”
`
`l’l3H30i8OZNEl
`
`.
`
`wan-9 sooztmvw
`
`
`
`0Z'€€3l53'WWl N0l.l‘lillilG s 09l[llI89ElZ30lS3 : 9096Zl85SlllG V 0lHl:lX.-l3‘0ldSll3liIiS : l3W!.l l‘l5!lll9Gl1l3l5?3l llld Z01Si'?9 CUDZIGZIG ll! M38 3 [W93 39Vd
`Stavrianopoulos at al., US. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 20 [Amendment Under 37 C.F.R. _§1.1 15 (In Response To The
`Novemberv26, 2004 Office Action) -- May 25, 2005]
`
`(Currently Amended) A non-porous glass or non-porous plastic solid
`Claim 3247.
`support comprising one or more amine s
`h drox l s or e oxide s thereon
`wherein at least one double-‘stranded nucleic acid is
`fixed or
`immobilized thereto; to said non— orous
`lass or non- orous
`lastic solid su
`ort
`via said one or more amineisl, hydroxyllsl or epoxide(sl, and wherein atireast one
`nucleic acid strand of said double—stranded nucleic acid has covalently attached
`
`thereto either:
`
`(i)
`(ii)
`
`a non-radioactive signaling moiety, or
`a non-radioactive bridging moiety covalently or non-covalently .
`attached to a non-radioactive signaling moiety
`
`
`
`(Currently Amended) The non-porous glassor non-porous plastic
`Claim 3248.
`solid support of claim gl_ai_n3_s 3246 or 3247, wherein said non-porous
Accessing this document will incur an additional charge of $.
After purchase, you can access this document again without charge.
Accept $ Charge
Still Working On It
This document is taking longer than usual to download. This can happen if we need to contact the court directly to obtain the document and their servers are running slowly.
Give it another minute or two to complete, and then try the refresh button.
A few More Minutes ... Still Working
It can take up to 5 minutes for us to download a document if the court servers are running slowly.
Thank you for your continued patience.
This document could not be displayed.
We could not find this document within its docket. Please go back to the docket page and check the link. If that does not work, go back to the docket and refresh it to pull the newest information.
Your account does not support viewing this document.
You need a Paid Account to view this document. Click here to change your account type.
Your account does not support viewing this document.
Set your membership
status to view this document.
With a Docket Alarm membership, you'll
get a whole lot more, including:
- Up-to-date information for this case.
- Email alerts whenever there is an update.
- Full text search for other cases.
- Get email alerts whenever a new case matches your search.
One Moment Please
The filing “” is large (MB) and is being downloaded.
Please refresh this page in a few minutes to see if the filing has been downloaded. The filing will also be emailed to you when the download completes.
Your document is on its way!
If you do not receive the document in five minutes, contact support at support@docketalarm.com.
Sealed Document
We are unable to display this document, it may be under a court ordered seal.
If you have proper credentials to access the file, you may proceed directly to the court's system using your government issued username and password.
Access Government Site
