MAY. 25. 2005 6: 08PM
`
`ENZO BIOCHEM
`
`RECEIVED
`CENTRAL FAX CENTER
`
`Attorney Docket No.: Enz-7(P)(C~~AY 2 5 Z005
`
`.. ...... .
`
`NO. 5419
`
`P. 1
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`In re Application of:
`
`Serial No.:
`
`Filed:
`
`For:
`
`08/486,070
`
`June 7, 1996
`
`Group Art Unit: 1631
`
`SPE: Ardin H. Marschel, Ph.D
`
`NUCLEIC ACIDS FIXED OR IMMOBILIZED TO NON-POROUS
`SOLID SUPPORT, AND SYSTEM, ARRAY AND NON-POROUS
`GLASS OR PLASTIC SOLID SUPPORT COMPRISING SUCH FIXED
`OR IMMOBILIZED NUCLEIC ACIDS
`
`Confirmation No.:
`
`CERTIFICA TE OF FACSIMILE TRANSMISSION
`
`Commissioner for Patents
`P.O. Box 1450
`Alexandria, VA 22313-1450
`
`Sir:
`
`I hereby certify that the attached correspondence (total of 137 pages
`
`including this sheet) comprising:
`
`Amendment Under 37 C.F.R. § 1.115
`(In Response To November 26, 2004 Office Action) [including Exhibits A, B & C]j
`
`and Request For Extension Of Time (3 Months)
`
`was sent to the United States Patent and Trademark Office by telefax to (703)
`872-9306.
`
`Date: May 25, 2005
`
`onald C. Fedus, Reg. No. 32,567
`Natalie Bogdanos, Reg. No. 51,480
`Attorneys for Applicants
`Enzo Life Sciences, Inc.
`c/o Enzo Biochem, Inc.
`527 Madison Avenue, 9th Floor
`New York, NY 10022-4304
`Telephone (212) 583-0100
`Facsimile (212) 583-0150
`
`C)
`""1:;J
`r:1
`
`i::
`-<
`2 ~
`(""),....."
`~ g c..n
`
`Enz-7(P)(C3)
`
`PAGE 1/131 t RCVD AT 5125/2005 6:06:41 PM ~astem ~ight rune] I SVR:USPTO.fFXRF·1/1 1 DNIS:87293D6 t CSID:212583D150 t DURATION (mmo$s):32-14
`
`Enzo Exhibit 2021
`BD v. Enzo
`Case IPR2017-00172
`
`Exhibit 2021 Page 1
`
`

`

`MAY. 25. 2005 6: 09PM
`
`ENZO B roCH EM
`
`NO. 5419
`
`P. 6
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`Applicant{s) :
`
`Stavrianopoulos, at al.
`
`Serial No.:
`
`08/486,070
`
`Group Art Unit: 1631
`
`Filed:
`
`June 7, 1995
`
`SPE: Ardin H. Marschel, Ph.D.
`
`For: NUCLEIC ACIDS FIXED OR IMMOBILIZED
`TO NON·POROUS SOLID SUPPORT, AND
`SYSTEM, ARRAY AND NON·POROUS GLASS)
`OR PLASTIC SOLID SUPPORT COMPRISING
`)
`SUCH FIXED OR IMMOBILIZED NUCLEIC ACIDS)
`------------------------------)
`
`VIA FILED BY FACSIMILE TRANSMISSION
`TO: (703) 872-9306
`
`Mail Stop -- Amendment
`Commissioner for Patents
`P.O. Box 1450
`Alexandria, VA 22313-1450
`
`527 Madison Avenue, 9th Floor
`New York, NY 10022-4304
`May 25, 2005
`
`AMENDMENT UNDER 37 C.F.R. §1.115
`(IN RESPONSE TO THE NOVEMBER 26, 2004 OFFICE ACTION)
`
`Dear Sirs:
`
`This paper (Amendment Under 37 C.F.R. § 1.115) is responsive to the
`
`November 26, 2004 Office Action mailed in connection with the above-identified
`
`application. A response to the November 26, 2004 Office Action was initially due
`
`by February 26, 2004. This paper is accompanied by a Request For Extension Of
`
`Time (Three Months), and authorization for the fee therefor. Upon the expected
`
`granting of Applicants' extension request, the response deadline will be extended
`
`to May 26, 2005. Accordingly, this paper is being timely filed.
`
`Enz-7(P)(C3)
`
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`
`Exhibit 2021 Page 2
`
`

`

`ENZO B JOe H EM
`MAY. 25. 2005 6:09PM
`P. 7
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 2 [Amendment Under 37 e.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`CERTIFICATE OF TRANSMISSION
`
`I hereby cl;lrtify that this paper (Amendment Under 37
`C.F,R. §1.115 (In Aespon$$ To The Novembar 26,2004
`Office ActiDn) (along with any documents referred to 85
`attached or encloGEKI) Is baing facsimile transmitted to
`the United States Patent and TrademBr
`(rax No. 703-
`872·9306) on May 2.5,
`'Yl*2 2~ lOoS'
`
`Date
`
`Amendments to the Claims are reflected in the listing of claims which begins on
`
`page 3 of this paper.
`
`Remarks begin on page 45 of this paper.
`
`Enz-1(PI (C31
`
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`
`Exhibit 2021 Page 3
`
`

`

`MAY. 25. 2005 6: 09PM
`ENZO B JOCHEM
`P. 8
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 3 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Amendments to the Claims: This listing of the claims below will replace all prior
`
`versions and listing of claims in this application.
`
`Usting of Claims:
`
`Claims 1-3143 (Canceled).
`
`Claim 3144. (Currently Amended) A non-porous solid support comprising one or
`
`more amine(s), hydroxyHs) or epoxide(s) thereon, wherein at least one single(cid:173)
`
`stranded nucleic acid is eJire9tI'l er indirectly fixed or immobilized thereto in
`
`hybridizable form to said non-porous solid support via said one or more amine(s).
`
`hydroxvl(s) or epoxide(s). r'tI'Iherein when said RHsleie aeld is indirestly fi~ced or
`
`imrneeilizBd ta said nen peF01:lS selid SUlSllSlert, said indirect thEatien ar iR'lR'lobilization
`is net t.o a 8BII fbEed .w situ to said naA perol;Js selid sl:l~fJert.
`
`Claim 3145. (Currently Amended) A non-porous solid support comprising one or
`
`more amine(s), hydroxyl(s) or epoxide(s) thereon, wherein at least one double(cid:173)
`
`stranded nucleic acid is fixed or immobilized thereto, to said non-porous solid
`
`support via said one or more amlne(s), hydroxyl(s) or epoxide(s), and wherein M
`
`least one nucleic acid strand of said at least one double-stranded nucleic acid has
`
`covalently attached thereto either:
`
`(j)
`
`Iii)
`
`a non-radioactive signaling moiety, or
`
`a non-radioactive bridging moiety covalentlY or non-covalently
`
`attached to a non-radioactive signaling moiety.
`
`GOFflf)r'ises at least ene non radieactive chemieal label which is Cifuantifiasle or
`
`eeteetaele, 'JHher:ein when saie nueleie aoiEl is indirectly fheee! or iR'lR'lobllizeei to said
`
`neR f:loreus SEllid SUISlf:lort, said indirect fixatien or' immobilization is RBt to a eell
`tbEed .w s#w to said AOA poroblS solia suppert.
`
`Enz-7(P)(C3)
`
`PA~ 81131 a RCVD AT 512512005 6:06:41 PM ~astem Daylight Tlll1e]· SVR:USPTO.fFXRF·111 1 DNIS:8n9306' CSID:2125830150 I DURATION (mm-ss):32.14
`
`Exhibit 2021 Page 4
`
`

`

`ENZO BIOCHEM
`MAY. 25. 2005 6: 10PM
`P. 9
`NO. 5419
`Stavrianopoulos et ai" U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 4 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3146. (Currently Amended) The non-porous solid support of eJ.atm claims
`
`3144.L ef 3145,3287.3324 or 3325, wherein said non-porous solid support
`
`comprises glass or plastic.
`
`Claim 3147. (Currently Amended) The non-porous solid support of eJ.a.i.FR claims
`3144L ,* 3145/ 3287, 3324 or 3325, wherein said non-porous solid support
`comprises a plate or plates, a well or wells, a microtiter well or microtiter wells, £!
`
`depression or depressions, a tube or tubes, a cuvette or cuvettes, or a bead or
`
`beads) er a -set of said plates, wells, elepressions, tubes, swvettes or beads.
`
`Claim 3148-3149. (Canceled).
`
`Claim 3150. (Currently Amended) The non-porous solid support of claim 3287 or
`
`3325 :31 q 9, ""herein saiel reactive sites or BiRding sites comprising sornprise one
`
`or more amines, l=1'ldro)@/ls or epo>ddes.
`
`Claim 3151. (Currently Amended) The non-porous solid support of wI-aiR:t claims
`
`3144.L GF 3145, 3287, 3324 or 3325, wherein said non-porous solid support has
`
`been treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3152. (Previously Presented) The non-porous solid support of claim 3151,
`
`wherein said surface treatment agent comprises an amine providing compound, an
`
`epoxy glue or solution, an acid solution, or ammonium acetate.
`
`Claim 3153. (Previously Presented) The non-porous solid support of claim 3151,
`
`wherein said blocking agent comprises Denhardt's solution.
`
`Enz-7(P)(C3)
`
`PAGE 91131 a RCVD AT 512512005 6:06:41 PM ~astem Daylight rone] a SVR:USPTO-EFXRF·111 & DNIS:8729306 * CSID:2125830150' DURATION (mm-ss):32.14
`
`Exhibit 2021 Page 5
`
`

`

`ENZO BroCHEM
`MAY. 25. 2005 6: 10PM
`P.
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`10
`
`Page 5 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 20051
`
`Claim 31 54. (Currently Amended) The non-porous solid support of eJ.ai.m claims
`
`3144,t. <* 3146,3287,3324 or 3325, wherein said elireet SF imfirest fixation or
`immobilization to said non-porous solid support is covalent.
`
`Claim 31 55. (Currently Amended) The non-porous solid support of ek:Hm claims
`
`3144,t.ef 3145r 3287,3324 or 3325, wherein said direst sr iAelireet fixation or
`
`immobilization to said non-porous solid support is non-covalent.
`
`Claim 3156. (Canceled).
`
`Claim 3157. (Currently Amended) The non-porous solid support of eJatm. claims
`
`3145 ( 3287 or 3325, wherein one strand of said at least one double-stranded
`
`nucleic acid is ineiireetly fixed or immobilized to said non-porous solid support by
`
`S8REhvieR R'Isriaizatien or l:ly hyarieiiaation te a eem[;:)leFFlemary and the other
`
`nl:leleie aeid strand of said at least one double-stranded nucleic acid is hybridized to
`
`said one strand.
`
`Claim 3158. (Currently Amended) The non-porous solid support of eJ.ai.m claims
`3144 or 3324, wherein said nucleic acid eomprises is DNA or RNA.
`
`Claim 3159. (Currently Amended) The non-porous solid support of claim claims
`
`3145, 3287 or 3325, wherein said nucleic acid SSFAflFises is DNA, RNA eF betR.
`
`Claim 3160. (Previously Presented) The non-porous solid support of claim 3144,
`
`wherein said nucleic acid comprises a nucleic acid sequence complementary to a
`
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`Enz-1(P)(C3)
`
`PAGE 101131- RCVD AT 512512005 6:06:41 PM ~astem Da~ight Timel l SVR:USPTO.£FXRf.1J1I ONIS:872930& I CSI0:2125830150 I DURATION !mm-ss):32.14
`
`Exhibit 2021 Page 6
`
`

`

`MAY. 25.2005 6: 10PM
`ENZO B JOCHEM
`NO. 5419
`P.
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`11
`
`Page 6 [Amendment Under 37 e.F,R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3161. (Currently Amended) The non-porous solid support of eJ.a.im claims
`
`3145 or 3287, wherein one strand of said at least one double-stranded nucleic acid
`
`comprises a nucleic acid sequence of interest sought to be identified, Quantified or
`
`sequenced.
`
`Claim 3162. (Currently Amended) The non-porous solid support of ~ claims
`3144 or 3324, wherein said single-stranded nucleic acid is unlabeled.
`
`Claim 3163. (Currently Amended) The non-porous solid support of eJa.i.ffi claims
`3144.L 9f 3145,3287,3324 or 3325, wherein said non-porous solid support is
`
`transparent or translucent.
`
`Claim 31 64. (Currently Amended) . The non-porous solid support of claim claims
`
`3145 or 3287, wherein said nOR raelieaetiva sA8R=tisal lal:Jel non-radioactive
`signaling moiety is quantifiable in or from a fluid or solution, said quantity being
`proportional to the amount or quantity of said lal3el Gr laeels non-radioactive
`
`signaling moiety.
`
`Claim 3165. (Currently Amended) The non-porous solid support of claim claims
`
`3145 or 3287 I Wherein said non-porous solid support is transparent or translucent,
`
`and said non raaiaaetive shemieal laBsl non-radioactive signaling mOiety is
`
`quantifiable in or from a fluid or solution or iF! ar thrGI;J§h from said non-porous solid
`support, said quantity being proportional to the amount or Quantity of said lal:Jel of
`labels non-radioactive signaling moiety.
`
`Enz-7(P}(C3)
`
`PAGE 111131 t RCVD AT 5J2512otl5 6:06:41 PM [Eastern Da~ight Timel' SVR:USPTO.£FXRF·111 t DNIS:8729306 1 CSID:2125830150 t DURATION (mrn-ss):32.14
`
`Exhibit 2021 Page 7
`
`

`

`MAY. 25. 2005 6: 10PM
`ENZO B JOCHEM
`P.
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`12
`
`Page 7 [Amendment Under 37 e.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) _. May 25, 2005]
`
`Claim 3166. (Currently Amended) The non-porous solid support of ~ claims
`
`3140. 3287 or 3320, wherein said nan raelioacti'/e eAemisaJ label non-radioactive
`
`signaling moiety comprises a chromagen or a chromagenic compound, a ealarea
`
`aye 8SFHfl9~Ad, a fluoF9§eA or a fluafesseAt eOFAI;)EHIAS, a sRemiluFAineseent
`
`e.eFAJ3ounel, a ehelatin€J eeffif3E:lllne, aA eni!ylT\e aF an SA2?(FRatie cemflollns, a
`coenzyme, Q£ i:Jiotin, iminoeietiA, a f!iaf3ten or a Ii~anel.
`
`Claim 3167. (Currently Amended) The non-porous solid support of eJ.a.im. claims
`3145, 3287 or 3325, wherein a non-radioactive signal from said non raelioastiye
`shemieol lalael non-radioactive sIgnaling moiety is quantifiable or detectable by
`
`photometric techniques, speetreflAstoffiotrie teshniquos, colorimetric tesRAiEt~es,
`
`fl~oFometFie tssRFliques or sRemilumineseent tssAniques.
`
`Claim 3168. (Currently Amended) The non-porous solid support of &J.a.i.m. claims
`
`3144 or 3324, comprising more than one single-stranded nucleic acid.
`
`Claim 31 69. (Currently Amended) The non-porous solid support of eJ.a+FR claims
`
`3145,3287 or 3325, comprising more than one double-stranded nucleic acid.
`
`Claims 3170 - 3171 (Canceled).
`
`Claim 3172. (Currently Amended) A system comprising a non-porous solid
`support which comprises one or more amine(s), hydroxvJ(s) or epoxide(s) thereon,
`
`wherein aA9 at least one single-stranded nucleic acid is eiFeetl'l er indirestJy fixed or
`
`immobilized thereto in hybridizable form to said non-porous solid support via said
`
`one or more amine(s), hydroxyJ(s) or epoxideCsl. , 'NRerein when saia A\::Ielaie aaid is
`
`iRe/iFactl.,. fbEes SF immebiliOlea to said nOR porous solie! s~f:'IJ3ert, saie! imJireet
`
`fi~Eati8R SF immebilizatieA is not te a sell fixeel fR 51tH t8 saiel nen pora~s-Golid
`
`Enz-7(P)(C3)
`
`PAGE 121131 * RCVD AT 512512005 6:06:41 PM [Eastern Da~ight Timel * SVR:USPTO.fFXRf·1I1 t DNIS:872930& * CSID:2125830150 l DURATION (mm-ssj:32.14
`
`Exhibit 2021 Page 8
`
`

`

`ENZO B JOCHEM
`MAY. 25. 2005 6: 11 PM
`P.
`NO. 5419
`Stavrianopoulos et aI., u.s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`13
`
`Page 8 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 20051
`
`Claim 3173. (Currently Amended) A system comprising a non-porous solid
`
`support which comprises one or more amine(s) ( hydroxyl(s) or epoxide(s) thereon,
`
`wherein -afH:i at least one double-stranded nucleic acid Is fixed or immobilized
`thereto, to said non-porous solid support via said one or more amine(s), hydroxylCsl
`
`or epoxide(s), and wherein at least one nucleic acid strand of said double-stranded
`
`nucleic acid has covalently attached thereto either:
`a non-radioactive Signaling moietyc or
`a non-radioactive bridging moiety covalently or non-covalently
`attached to a non-radioactive signaling moiety.
`
`Iii)
`
`(i)
`
`o8FAf3rises at least or:\e non raElioaetive ehemisallabel v/hioR is ~l:JaR1:i:f'iable or
`deteetable, ane wherein wRen saiel Al;loleio aeid is lnelireotl'l fi:x:ea er iFFlFAOeili,es ~e
`
`aaia non ""OFOI;IS selia sl:lJ3J3eFt, said indiFeet fixation or imFRebilizatien is not to a eell
`
`fixeellR sitl;l te saiel non ""orel:ls selie! 5ul'l""ert.
`
`Claim 3174. (Currently Amended) The system of eJ.ai:m. claims 3172.t. eF 3173,
`
`3279, 3280. 3281 or 3282, wherein said non-porous solid support comprises glass
`
`or plastic.
`
`Claim 3175. (Curr~ntly Amended) The system of 6Jai.m claims 3172.t. eF 3173,
`3279, 3280. 3281 or 3282. wherein said non-porous solid support comprises a
`plate or plates, a well or wells, a microtiter well or microtiter wells, a depression or
`
`depressions, a tube or tubes, a cuvette or cuvettes, or a bead or beads, eF a set of
`
`oaiEi ""Iates, 'Neils, eef3ressions, ll:lbes, el;lvet1:es eF beaEis.
`
`Claims 3176-3178. (Canceled),
`
`Enz-7(PHC3)
`
`PAGE 131131 * RCVD AT 512512005 6:06:41 PM (Eastern Da~ight Time) t SVR:USPTO.£FXRF·1I1 * DNIS:8729306' CSID:2125830150 a DURATION ImJlloSS):32.14
`
`Exhibit 2021 Page 9
`
`

`

`MAY. 25. 2005 6: 11 PM
`ENZO B IOCHEM
`P. 14
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08f486,070 (Filed June 7, 1995)
`
`Page 9 [Amendment Under 37 C.F.R. §1.115 (In Response To The
`November 26, 2004 Office Action) •• May 25, 2005]
`
`Claim 3179. (Currently Amended) The system of ~ claims 3172L &F 3173,
`3279r 3280r 3281 or 3282, wherein said non-porous solid support has been
`
`treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3180. (Previously Presented) The system of claim 3179, wherein said
`
`surface treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3181. (Previously Presented) The system of claim 3179, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Claim 3182. (Currently Amended) The system of eJaim. claims 3172L eF 3173,
`3279r 3280, 3281 or 3282. wherein said direst SF inelireSf fixation or
`
`immobilization to said non-porous solid support is covalent.
`
`Claim 3183. (Currently Amended) The system of GJaH:R claims 3172.1. ef 3173,
`
`3279, 3280, 3281 or 3282, wherein said diFect or indirect fixation or
`
`immobilization to said non-porous solid support is non-covalent.
`
`Claim 31 84. (Canceled).
`
`Claim 3185. (Currently Amended) The system of ~ claims 3173, 3281 or'
`3282, wherein saie one strand of said double-stranded nucleic acid is iF\direetly
`fixed Or' immobilized to said non-porous solid support ey samh'vieA A't'eriei2lation or
`19.,. hyerielizatien 1:0 a e9R!1~leFflen1;ary and the other nucleic acid strand of said
`
`double-stranded nucleic acid is hybridized to said one strand.
`
`Enz·7(PHC3)
`
`PAGE 141131 t RCVD AT 512512005 6:06:41 PM [Eastern Oa~ight Timel t SVR:USPTO-EFXRF,1I1 t ONIS:8729306 a CSID:2125830150 I DURATION (mrn-ss):32.14
`
`Exhibit 2021 Page 10
`
`

`

`MAY. 25:2005 6:11PM
`
`ENZO B lOCHEM
`
`P. 15
`NO. 5419
`Stavrianopoulos et a!., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 10 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3186. (Currently Amended) The system of ~ claims 3172 or 3280,
`
`wherein said nucleic acid i.§. eeffi~Fises DNA aF RNA.
`
`Claim 3187. (Currently Amended) The system of eJ.atm. claims 3173, 3281 or
`3282, wherein said nucleic acid is GeFA~rises DNA, RNA, 9r se.tR..
`
`Claim 3188. (Previously Presented) The system of claim 3172, wherein said
`
`nucleic acid comprises a nucleic acid sequence complementary to a nucleic acid
`
`sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3189. (Currently Amended) The system of GJ.ai.m. claims 3173 or 3288,
`
`wherein one strand of said at least one double-stranded nucleic acid comprises a
`
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3190. (Previously Presented) The system of claim 3172, wherein said
`
`single-stranded nucleic acid is unlabeled.
`
`Claim 3191. (Currently Amended) The system of ~ claims 3172L eF 3173,
`3279, 3280, 3281 or 3282, wherein said non-porous solid support is transparent
`
`or translucent.
`
`Claim 3192. (Currently Amended) The system of eIaim claims 3173 or 3288,
`
`wherein said Ilen raaioaetive sFtemieal label non-radioactive Signaling moiety is
`
`quantifiable in or from a fluid or solution, said quantity being proportional to the
`
`amount or quantity of said label SF labels non-radioactive Signaling moiety.
`
`Enz-7(P)(C3)
`
`PAGE 151131 3 RCVD AT 512512005 6:06:41 PM ~astem Da~ight Time] I SVR:USPTO.fFXRF.111I DNIS:8729308 R CSID:2125830150 I DURATION (mll»S):32.14
`
`Exhibit 2021 Page 11
`
`

`

`ENZO B IOCHEM
`MAY. 25. 2005 6: 11 PM
`P.
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7. 1995)
`
`16
`
`Page 11 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) .~ May 25, 2005]
`
`Claim 3193. (Currently Amended) The system of ~ claims 3173 or 3288,
`
`wherein said non-porous solid support is transparent or translucent, and said AeA(cid:173)
`
`raeHoaetive sl=1emieal label non-radioactive signaling moiety Is quantifiable in or from
`
`a fluid or solution or i-A-er through from said non-porous solid support, said quantity
`
`being proportional to the amount or quantity of said label or labels non·radioactive
`
`signaling moiety.
`
`Claim 3194. (Currently Amended) The system of &IaifR. claims 3173, 3281 or
`
`3282, wherein said AeA raaioastive ehemisal label non-radioactive signaling moiety
`comprises a chromagen or a chromagenic compound, a Golarea a'te eOFAJ:)o~F1a, a
`flu8Fe90n or a fll:loreseent GeFR~OUAa, a shsmilumiAesGent semj3ounel, a sRelatiRg
`
`GOFApound, an enzyme or aA eAzymatis sOR'lpound, a seen:Z:yFAe, or biotir.,
`
`iminebiotin, a hapten sr a liijana.
`
`Claim 3195. (Currently Amended) The system of eJa+ffi claims 3173, 3281 or
`
`3282, wherein a non-radioactive signal from said Aon raeieaetlve eheFRisal label
`
`non-radioactive signaling moiety is quantifiable or detectable by photometric
`
`techniques, sJ:leetreJal=lotemetrie tesAniques, selorimetrie teehAiElues. fh::loFometrie
`
`teehniql:Jes or GhemilumineseeFtt tesRniques.
`
`Claim 3196. (Previously Presented) The system of claim 3172, comprising more
`
`than one single-stranded nucleic acid.
`
`Claim 3197. (Currently Amended) The system of maim claims 3173, 3281 or
`
`3282, comprising more than one double·stranded nucleic acid.
`
`Enz-7(PUC3)
`
`PAGE 161131 t RCVD AT 512512005 6:06:41 PM ~astem Da~ight Timel l SVR:USPTO-EFXRF.1I1I DNIS:8729306· CSID:2125830150 & DURATION (mlJloSS):32.14
`
`Exhibit 2021 Page 12
`
`

`

`MAY. 25. 2005 6: 12PM
`ENZO B IOCHEM
`P.
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`17
`
`Page 12 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005}
`
`Claim 3198. (Currently Amended) An array comprising various single-stranded
`nucleic acids elirostly SF inairectly fixed or immobilized in hybridizable form to a non(cid:173)
`porous solid support, ',,,,'herein wf:1eA Baiel nl;loleis aoids are inaires:t:ly thEea SF
`
`immebilizea ta ssiel nan paraus salia Sl::IraraaFt, said inelireet fixa1:ien aF immabiliaatien
`is nat 1:9 a eell tixeEi if} situ tasaia Rem parous selia sl::Jppert.
`
`Claim 3199. (Currently Amended) An array comprising various double-stranded
`
`nucleic acids eliFeetly aF indirestly fixed or immobilized to a non-porous solid
`support, wherein at least one nucleic acid strand of said various double-stranded
`
`nucleic acids comprises at least one non-radioactive sAemieal label signaling moiety
`which is quantifiable or detectable , anel ""JheFein ... vhen said nueleiG aeies are
`indiros:t:ly fixed aF iffim9bilized 1;9 saiEl Rsn f:l0fSUS selie sl::IJlJgert, saiel iAelirest
`fh{atien SF iFRfR8eilizatien is not t8 a cell 'bEed iR sfH:i to soia Ren I3Sr9l:lS ooHa
`
`Claim 3200. (Currently Amended) The array of ~ claims 3198,L e.f 3199,
`
`3300, 3301« 3326 or 3327 c wherein said non-porous solid support comprises glass
`
`or plastic.
`
`Claim 3201. (Currently Amended) The array of ~ claims 3198L Sf 3199,
`3300. 3301 r 3326 or 3327 r wherein said non-porous solid support comprises a
`
`plate or plates, a well or wells, a microtiter well or microtiter wells, a depression or
`
`depressions, a tube or tubes, a cuvette or cuvettes, or a bead or beads, or a set of
`
`said Jillates, wells, elepressiBAs, tUBes, euvettes or eeaels.
`
`Claim 3202 -3203. (Canceled).
`
`Enz-1(P)(C3)
`
`PAGE 17/131* RCVD AT 512512005 6:06:41 PM ~astem Da~ight runel a SVR:USPTO-EFXRF.1I1 a DNIS:8729306 a CSID:2125830150 a DURATION (mm-ssj:32.14
`
`Exhibit 2021 Page 13
`
`

`

`MAY. 25. 2005 6: 12PM
`ENZO B JOCHEM
`P.
`NO. 5419
`Stavrianopoulos et aI., U.S. Pat. Appl. Serf No. 08/486,070 (Filed June 7, 1995)
`
`18
`
`Page 13 [Amendment Under 37 C.F.A. § 1.115 (In Response To The
`November 26, 2004 Office Action) f t - May 25, 2005]
`
`Claim 3204. (Currently Amended) The array of claims 3198, 3199, 3300, 3301,
`3326 3327 claim a20a, ' .... l:IereiR sais reactive sitos or biRding-sites comprise
`comprising ono or more amines, h'lelro)(yls or B)30)Eieies.
`
`Claim 3205. (Currently Amended) The array of ~ claims 3198,L ef 3199,
`3300, 3301, 3326 or 3327. wherein said non-porous solid support has been
`treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3206. (Previously Presented) The array of claim 3205, wherein said surface
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3207. (Previously Presented) The array of claim 3205, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Claim 3208. (Currently Amended) The array of e.J.afm. claims 3198L &F 3199,
`3300, 3301, 3326 or. 3327, wherein said direct OF indir=eet fixation or
`
`immobilization to said non-porous solid support is covalent.
`
`Claim 3209. (Currently Amended) The array of elafm. claims 3198L ef 3199,
`3300, 3301 ( 3326 or 3327! wherein said direst er indireet fixation or
`
`immobilization to said non-porous solid support is non·covalent.
`
`Claim 3210. (Canceled).
`
`Enz-7(P)(C3)
`
`PAGE 181131 1 RCVD AT 512512005 6:06:41 PM ~astem Da~igflt Timel' SVR:USPTO.fFXRF·1f1' DNIS:8729305* CSID:2125830150 t DURATION (mlJloSS):32.14
`
`Exhibit 2021 Page 14
`
`

`

`ENZO BIOCHEM
`MAY. 25.2005 6:12PM
`P.
`NO.5419
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`19
`
`Page 14 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 26, 2005]
`
`Claim 3211. (Currently Amended) The array of £f.a+m. claims 3199 or 3301,
`
`wherein one stranEj the first strands of said double-stranded nucleic acids 6f
`
`seEilolenees-is srn;! iRelirestly fixed or immobilized to said non-porous solid support &y
`
`eanEhvisR l=Iybridizatien OF by l=IybriaiilostioA to s9mplementaF¥ and the other nucleic
`
`acid strands of said double-stranded nucleic acids are hybridized to said fjrst
`
`strands.
`
`Claim 3212. (Currently Amended) The array of etaHTl claims 31 98 or 3300,
`
`wherein said nucleic acids ~sol'l1prise DNA or RNA.
`
`Claim 3213. (Currently Amended) The array of clatm claims 3199 or 3301,
`
`wherein said nucleic acids i§..eoFl'l,uise DNA~Nl\i or 98th.
`
`Claim 3214. (Currently Amended) The array of ~ claims 3198 or 3289,
`
`wherein said nucleic acids comprise nucleic acid sequences complementary to
`
`nucleic acid sequences of interest sought to be identified, quantified or sequenced.
`
`Claim 3215. (Currently Amended) The array of eJ.a..i.m. claims 3199 or 3290,
`
`wherein one strand of said double-stranded nucleic acids comprises a nucleic acid
`
`sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3216. (Currently Amended) The array of eJ.atm claims 3198 or 3300,
`
`wherein said single-stranded nucleic acids are unlabeled.
`
`Claim 3217. (Currently Amended) The array of elaim claims 3198 L Sf' 3199,
`
`3300, 3301, 3326 or 3327 r wherein said non-porous solid support is transparent
`
`or translucent.
`
`Enz-7(PHC3)
`
`PAGE 19/131 1 RCVD AT 512512005 6:06:41 PM [Eastern Da~jght Timel ~ SVR:USPTO.£FXRF·1f1 ' DNIS:87293061 CSID:2125830150· DURATION (mrn-ss):32.14
`
`Exhibit 2021 Page 15
`
`

`

`MAY. 25. 2005 6: 12PM
`ENZO B IOCHEM
`NO. 5419 P.20
`Stavrianopoulos at aI., U.S. Pat. Appl. Ser. No. 08f486,070 (Filed June 7,1995)
`
`Page 15 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3218. (Currently Amended) The array of claifR claims 3199 or 3290,
`
`wherein said non-radioactive ehsm;eal label signaling moiety is quantifiable in or
`
`from a fluid or solution, said quantity being proportional to the amount or quantity
`
`of said label or labels.
`
`Claim 3219. (Currently Amended) The array of eJ.afm claims 3199 or 3290,
`
`wherein said non-porous solid support is transparent or translucent, and said non(cid:173)
`
`radioactiv.e ehemieal labol signaling moiety is quantifiable in or from a fluid or
`
`solution or in SF through from said non-porous solid support, said quantity being
`
`proportional to the amount or quantity of said laeel or labels non·radioactive
`
`Signaling moiety.
`
`Claim 3220. (Currently Amended) The array of GJ.aH:A. claims 3199, 3301, 3326 or
`
`3327 r wherein said non-radioactive ehemieal laeol signaling mOjety comprises a
`chromagen or a chromagenic compound.,..a--selored dye eompo\;fna, a fluoFogen or a
`fl-tJor9scent eompollnd, a sheFFlih,iffiineseeFlt compound, a GRolatin\i GOFFlpeUna, aA
`enzYFAO or an 8nzymatie eompouArh a soenZYffie, or eiotin, iminol:iliotin, a hapten SF
`a Iigane.
`
`Claim 3221. (Currently Amended) The array of GkHm claims 3199, 3301! 3326 or
`
`3327, wherein a non-radioactive signal from said non·radioactive ehemioallaael
`
`Signaling moiety is quantifiable or detectable by photometric techniques,
`
`speetropAotometric teehniElues, oolorimetric teehniEluos, fluSFometrio teehniques or
`
`sAemilumineseent teehniElues.
`
`Enl-7(P)(C3)
`
`PAGE 201131 t RCVD AT 512512005 6:06:41 PM ~astem Da~ight Timep SVR:USPTO.£FXRf.111I DNIS:8729306* CSID:2125830150 I DURATION ImflloSS):32.14
`
`Exhibit 2021 Page 16
`
`

`

`MAY. 25. 2005 6: 13PM
`
`ENZO B JOCHEM
`P.
`NO. 5419
`Stavrianopou[os et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`21
`
`Page 16 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November 26, 2004 Office Action) -- May 26, 2005] ,
`
`Claim 3222. (Currently Amended) An array comprising various single-stranded
`
`nucleic acids directly or ineiirestl', fixed or immobilized in hybridizable form to a non(cid:173)
`
`porous solid support having wells or depressions, wherein 'NMen said Al:Joleio aeids
`
`are IRdir:e6~1't' fines or immobilized to said \,,1 ell 9 or aef;'lrsssions of said nOR porol:ls
`
`solid sl:Jf'l~ert( s~id ;AsireGt fixation or iFflm9BiIlzation is Rot to a eell fixes fn situ to
`saia 'Neils or aef;'lressions af said non f;'I9FOUS solia Sl:IPJ*)ft.
`
`Claim 3223. (Currently Amended) An array comprising various double-stranded
`
`nucleic acids direetly or inaireetly fixed or immobilized to a non-porous solid support
`
`having wells or depressions, wherein at least one nucleic acid strand of each of
`
`said various double-stranded nucleic acids comprises at least one non-radioactive
`
`e.J:lel'flieal label signaling moiety which is quantifiable or detectable, anE! 'A'hereiA
`
`when saiEJ Aucleis asids are indir8s11y fheee or immel3ili2:ed to saiEi lo'Volls or
`
`eeJ3ressiofls of said non f'l0Fe~S solie S~f;)f;)ert, said indirect 1ixa:f:isA or imFFlobililatiofl
`
`is Rot to a sell flxeEi jn s/w 10 saia wells OF def,3ressions af saia Aon JilOre\:l9 solia
`
`Claim 3224. (Currently Amended) The array of ~ claims 3222 or 3223,
`
`wherein said non-porous solid support comprises glass or plastic.
`
`Claim 3225 - 3226. (Canceled).
`
`Claim 3227. (Currently Amended) The array of claims 3222 or 3223 elaiRt 222e,
`
`" ... herein said reaetive sites SF l=JinaiR~ sites eamprise wherein said non-porous solid
`
`support comprises one or more amines, hydroxyls sr epoxides.
`
`Enz-7(P){C3)
`
`PAGE 21/131 1 RCVD AT 512512005 6:06:41 PM [Eastern Da~ight Timel a SVR:USPTO.fFXRF·1/1 1 DNIS:8729306 -CSID:2125830150 a DURATION Imm-ss):32.14
`
`Exhibit 2021 Page 17
`
`

`

`MAY. 25. 2005 6: 13PM
`ENZO B IOCHEM
`NO. 5419 P.22
`5tavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995J
`
`Page 17 [Amendment Under 37 C.F.R. § 1.115 (In Response To The
`November ·26, 2004 Office Action) -- May 25, 2005]
`
`Claim 3228. (Currently Amended) The array of eJa1m. claims 3222 or 3223,
`
`wherein said non-porous solid support has been treated with a surface treatment
`
`agent, a blocking agent, or both.
`
`Claim 3229. (Previously Presented) The array of claim 3228, wherein said surface
`
`treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution or ammonium acetate.
`
`Claim 3230. (Previously Presented) The array of claim 3228, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Claim 3231. (Currently Amended) The array of eIittm claims 3222 or 3223,
`
`wherein said direet SF indireet fixation or immobilization to said non-porous solid
`
`support is covalent.
`
`Claim 3232. (Currently Amended) The array of eIai-f:R. claims 3222 or 3223,
`
`wherein said direst ar indirest fixation or immobilization to said non-porous solid
`
`support is non-covalent.
`
`Claim 3233. (Canceled).
`
`Claim 3234. (Currently Amended) The array of claim 3223, wherein ane s:l:rand
`
`the first st