November 22, I999
`
`THE SCIENTIST - I7
`
`New l.|ns on THE BI.oc|(
`
`LABCONSUMER ANNUAL REVIEW or THERMAL CYCLERS
`Bv GRANT MEISENHOLDER
`
`:0.3"C and can be controlled by
`block, sample, or algorithm meth-
`ods. A 1/-'l—sizc VGA screen pro»
`Vidcs a sitnplified graphic interface
`and illustrates the cycling run.
`Real—time PCR is possible using
`the optional optical module.
`fully
`The Gene Cyclerm is
`licensed for PCR and boasts -.1 small
`footprint. Pelrier heating and fan
`cooling keep cycling rates high, and
`temperature accuracy is ensured
`through software algorithms. A
`heated lid is standard and 100 pro»
`grams can bc stored in memory.
`
`
`
`CeEheid's SmartCycIer
`
`
`
`heated lids: standard (HLDX) and an ultralow condensa-
`tion configuration model (HLDHP).
`The PowcrBlock I (single block) and H (dual block)
`offer Pelrier heating and a small footprint. Two program-
`ming modes facilitate ease of use for simple (“Basic”) and
`advanced (“Linl<") applications. The singlc—blocl: model
`can be upgraded to dual blocks. Heated lids are optional.
`The water-cooled Singlefilock and DualBlock systems
`are the workhorses of Ericomp's line, with the flexibility to
`accommodate 0.5- and O.2—ml
`tubes, microplates, and
`slides. The low-cost systems featurc NTST traceable accu-
`racy, a five—year warranty, and across—the-block tempera-
`ture uniformity of :0.2°C. Optional items include heated
`lids and various block configurations.
`
`G-enex Lid. The small, uncomplicated Pacer cycler from
`Genex comes with a start and stop button and three LEDS
`to indicate cycling stages. This simple configuration is pos-
`sible because all of the programming and storage is per-
`formed on an accessory PC with the proprietary Preceptor
`software and then transferred to the machine before each
`run. Program storage space is limited, therefore, by the
`size of the computer rather than the cycler’s on—board
`memory. The Pcltier-cooled block can hold 16 05-1111 or
`20 0.2-ml tubes, two regular slides, or a mini 96-well plate.
`
`GL Applied Research The Genetic Thermal Cyclcr
`GTC-2 features two- and three—stcp amplification ability,
`built»in programmable autoextension, storage of up to 99
`protocols, and a 4-8-place sample block, among other fea-
`tures. Available options include a heated lid, a 96-well plate
`adapter, an 89-place sample block. that can hold up to 48
`D,5—ml and -‘l-l 0.Z~ml tubes, and a low—ternpcratI.u'c module.
`
`Hyba.-‘d Hybaid has just launched its Multibloclt system to
`complement
`three already-established, stylish,
`individual
`cyclers that fit a variety ofneeds. All ofits machines are PCR
`licensed, Pclticr cooled, and calibrated to NTST standards.
`The attractive PCR Sprint is claimed to be the lowest-
`priced licensed model available according to company lit-
`eratnre. It features fast ramping of up to 3°C/second,
`temperature control from 4—99°C, user-friendly program-
`ming, interchangeable blocks for 0.5 and 0.2 ml tubes, and
`a rli."ce—_vear guarantee. Hybaid’s PCR Express speeds up
`reactions involving its AGS Gold DNA polymerase with a
`onc»secon(l dwell time. Five control modes allow protocol
`flexibility—activc/simulated tube, simulated slide, and
`simulated block. An autoadjusting heated lid allows use of
`any plasticware and oil—f1-ee PCR in tubes and plates.
`Alphanumeric programming and advanced editing features
`simplify protocols, and four interchangeable blocks allow
`use of 0.2-rnl, 0.5—ml, .flat—l)l0cl(, and 38-l-well formats.
`The Ornn—F. cyclcr is a compact, multiformat, portable
`reactor with the ability to acconunodate 0.2-ml, 0.3-ml,
`and 0.5--ml tubes as well as 96-well plates. Active tube
`control and dynamic uniformity of 205°C across the plate
`allow precision and reproducibility between samples and
`experiments. An optional heated lid is available for oil-
`free cycling.
`In rim PCR protocols can be performed en masse with
`the Omnislide system, allowing simultaneous processing of
`THERMO FISHER EX. 1013
`THERMO FISHER EX. 1013
`
`EpEendorf’s Mastercycler gradient model
`
`Cepheid The SrnartCycler'" uses a unique cooling/he-ar-
`ing/optics/reaction module (the LCOREW) to perform
`amplification reactions with real-time monitoring. The
`company is working on modules that will handle all phas»
`es of sample preparation, reaction, and analysis, reducing
`the possibility of contamination.
`Corbett Research The new Rotor-Gene Real-Time
`Thermal Cyclet has two separate light sources to prevent
`spectral overlap in dual fluorescence studies. Samples can
`be loaded with reagents in the cap and reaction mix in the
`bottom of the tube. Centrifugal action mixes the samples
`and allows temperature uniformity. A 96-well adapter for
`use with Gcne—Disks will soon be available. Corbett also
`manufactures a Gradient ‘)6 VVell Cycler for microplatcs
`and a High Speed Capillary Cyclcr, as well as Cooled and
`Ambient 96 VVell Cyclers.
`
`Eppendor! Scientific Instruments The Mastercycler®
`series is sold with the PCR license. The company's gradi-
`ent model was the first on the market and offers a 96-
`position universal block that can accept 96 0.2-ml tubes, 77
`0.5-ml tubes, or one 96-well plate. Functions inc-udc selec-
`table gradients (from 1 to 20°C), Peltier heating, adjustable
`ramping, time extension, touchdown, program linking, and
`pause. A heated lid is
`included. According to Vincent
`Prezioso, product manager at Eppendorf Scientific,
`the
`uniform-rate cooling aspect allows users to accurately
`reproduce optimized conditions for each cycling pattern.
`The standard version Mastcrcycler includes all of the
`same features as the gradient model (without the gradient).
`The Mastercycler personal has a 25—posi'r_ion universal
`block and offers most of the features on the standard
`model. Personal memory cards allow users to program and
`retain their own cycle profiles and transfer them between
`any of the Mastcrcyclcr workstations.
`
`Ericomp The DeltaCyclcr T (single block) and H (dual
`block) systems are compact in design and use thermoelec-
`tric heating and cooling to minimize the accessory tubing
`that water—coolcd models require. A range of 4-“C to I 10°C
`with a temperature uniformity of :0.2°C allows the full
`range of protocols to be used. Options include two types of
`
`S 0 much has changed in the last l1alf—decadc in the
`
`world of gene ainplification. New breakthroughs and
`protocols have exceeded scientists’ wildest expecta-
`tions for applications of this essential technology. Is there a
`single life science lab in the country without a thermal
`cycler? From the high—end, ultIafast light—based cyclers to
`the tiny personal cyclers, there is a cyclcr to fit every need.
`VVay hack in the early years of PCR, “doing an amplifi-
`cation” meant simply that
`increasing the concentration of
`some target sequence of interest
`to use in insertion/
`ligation reactions. Quickly enough, scientists developed new
`ways to use the cycler. Now PCR-based methods exist for
`every aspect of biology that relies on genetic information.
`Forensic, genomic, and evolutionary studies have ben-
`efited greatly from the technique known as random ampli-
`fied polymorphic DNA (RAPD) PCR, which is used to
`fingerprint species from genomic DNA without prior
`sequence knowledge. Using arbitrary oligos, two Cycles of
`PCR with low soingcnty are followed by formal high-
`stringency PCR with specific primers. The reaction is then
`followed by RFLP or comparative analysis.
`Touchdown PCR was developed to increase the yield of
`specific products. in this method, the annealing tempera-
`ture is lowered one degree every second cycle to a lower
`limit or “touchdown" temperature. This touchdown tem-
`perature is then used for 10 cycles. This process ostensibly
`enriches for a specific product by taking advantage of dif-
`ferences in melting temperatures between correct and
`incorrect products by twofold per cycle.
`ll/iore recently,
`this technique has been adapted for genomic sequencing to
`elucidate DNA sequences from known peptide sequences.
`Long PCR is a method that allows amplification of
`long stretches of DNA, from 0.5 to 20 kb. An enzyme mix
`containing both proofreading and polymerase activities
`allows specific extension over great distances. Wl'1c11 used
`as an alternative to RFLP analysis, it shortens the process
`from six to eight weeks down to hours or days. It also may
`be used as an alternative to variable number of tandem
`repeats (VNTR) analysis.
`Chromosomal analysis has also benefited from PCR.
`DOP-PCR is a method that allows uniform amplification
`of sequences across a genome. Comparative DOP-PCR is
`used to detect chromosomal imbalances in genetic diseases
`and evolutionary studies. Amplified fragment length poly-
`morphism-PCR (AFLP-PCR) is used to detect allelic loss
`in aging and other studies. Single nucleotide polymor-
`phism (SNP) detection has made great strides with PCR.
`For hybrid cell studies researchers have developed Alu-
`PCR to detect human chromosomes in the hybrid cells.
`Scientists involved in the Human Genome Project
`have made great use of the cycle sequencing method.
`Developed as an alternative to the Sanger method, PCR-
`based sequencing uses femtomolar amounts of primers to
`start
`the elongation PCR,
`followed by sequencing. A
`major advantage is that this method can be used for linear
`double—stranded DNA, unlil<c the Sanger method. lt can
`also be used for cosmid, plaque, and colony DNA
`sequencing.
`Scientists studying RNA cxprcssion have not been left
`out of the PCR craze. Using reverse transcriptasc to pro»
`duce CDNA gave rise to RT—PCR. This method has been
`used to diagnose a variety ofinfectious and genetic diseases.
`Histologists quickly realized the advantage of having
`precise control over temperature and incubation time and
`adapted the cycler for use with 2'72 situ hybridization. One
`limitation oftraditional ISH is detection of low-copy num»
`her RNAs. This was overcome by the development of
`cycling—primed it: rim (cycling PRINS) labeling, which
`allowed researchers to gain deeper insight into the more
`ephemeral processes of the cell.
`PE Biosystems holds the exclusive license rights to the
`PCR technology. Anyone who wishes to perform automat-
`ed PCR using certain reagents and machines must first
`obtain a license. Fortunately, many reagents and machines
`include these rights in the purchase price, but the user
`must check with the supplier before performing the ampli-
`fication. This applies to research as well as applied fields. If
`the license is not supplied, PE Biosystems will provide one
`for a fee.
`This year’s field of cyclers includes many familiar
`names as well as some ingenious new models.
`
`Bio-Rad Bio—Rad’s newest model is the iCyclcr”‘, which
`boasts NIST accuracy over its full temperature range of
`
`

`
`I8 -THE SCIENTIST
`
`THERMAL CYCLERS
`
`November 22, I999
`
`COMPANY
`
`Pnooucr
`
`SP/icE REQUIRED
`WxDxH (cM)
`27x19x16
`GeneCycler‘"'
`10x21x9
`icycler”
`
`
`
`THERMAL
`CONTROL
`
`PCB
`LICENSE moms
`Yes
`Yes
`
`
`
`PnocnAMs/
`$TEPS/ CvcLEs
`100/5/300
`255
`
`|'!EAT HATE
`( G/sEc)
`1.5
`3.0
`
`Cg0L HATE
`( C/sEc)
`0.8
`2.0
`
`THERMAIE
`RAuoE( C)
`Amb—97
`4-105 90.3
`
`BASE
`Pmca
`Call
`
`100/99/99
`Peltier
`Yes
`
`Peltier
`Yes
`100/99/99
`
`Peltier
`Yes
`100/99/99
`
`Peltier
`No
`26/99/99
`4-110 10.5
`
`Peltier
`No
`26/99/99
`4-110 10.5
`Peltier
`No
`26/99/99
`4-110 -20.4
`Peltier
`No
`26/99/99
`4-110 30.4
`Resistivelwater
`No
`26/99/99
`Amb—1 10 :05
`Resistive/water
`No
`26/99/99
`Amt>—1 10 20.5
`
`
`
`
`
`Peltier
`
`Unlimited
`
`26x41x27
`26x41x27
`18x35x25
`
`
`
`32x33x19
`32x44x19
`32x33><19
`32x33x19
`36x41x15
`36x51x15
`
`Cali
`
`Enpendori Scientific Inc.
`800-421-9988
`www.eppendortsi.com
`
`
`
`MastercycIer®
`Mastercycler gradient
`Mastercycler personal
`
`Ericomp
`800-541-8471
`www.ericomp.com
`
`
`
`
`
`Genex
`
`44 1737 556617
`www.genex.ltd.uk
`
`Deltacycler I System
`Deltacycler ll System
`PowerB|ock i system
`PowerBlock ll System
`Sing|eBlock System
`TwinBlock System
`
`Pacer
`
`4-99 10.4
`4-99 :0.4
`
`
`
`4-99 10.5
`Amb +10 to 99
`
`
`
`
`99 programs
`
`>100/10/99
`99/10/99
`
`
`
`60/5/99
`36/10/99
`
`99/-/99
`
`Yes
`Yes
`
`Yes
`Yes
`
`GL Applied Research inc.
`847-223-2220
`members.aoI.com/glapplied/
`
`homepagehtm
`
`
`GTC-2 Genetic Thermal Cycler
`46x63x48
`Resistive/water
`
`
`
`
`Peltier
`Call
`Mu|tiBl0ck
` Hybaid
`
`888-449-2243
`Peltier
`PCR Express
`23x39x25
`www.hybaid-us.com
`Peltier
`18x30x23
`PCR Sprint
`
`Omn-E
`Electric/fan
`19x28x19
`
`
`
`
`
`
`
`litaho Technology
`Rapidcycler
`30x23x37
`
`800-735-6544
`www.idah0tec.c0m
`
`Light
`
`
`
`TC 1600
`
`Call
`
`Electric
`
`Call
`
`Call
`
`
`
`
`
`
`
`
`
`
`
`
`
`Smartcyclerw
`
`30><30x22
`
`DEM
`
`150 programs
`
`
`
`
`
`Bio-Rad
`800-424-6723
`wvvw.bio-rad.com
`
`cepheiii
`408-541-4191
`www.cepheid.com
`
`
`
`
`
`OEM
`
`Call
`
`Call
`
`
`
`
`
`
`
`
`
`No
`No
`No
`No
`
`Yes
`Yes
`Yes
`Yes
`
`Yes
`
`No
`
`Yes
`Yes
`Yes
`
`Intelligent Bio-Instruments
`617-354-3830
`wvvw.inteIligentbio.com
`
`Peltier—.Joule
`Peltier-Joule
`Peltier-Joule
`Peltier
`
`37x55x25
`24x33x24
`22x28x11
`24x28x27
`
`
`
`MJ Research
`888-735-8437
`www.mjr.com
`
`
`
`
`
`Tetrad
`DNA Engine Series
`Minicycler
`FTC-100
`
`DNA Thermal Cycler 480
`GeneAmp 2400
`GeneAmp 9600
`GeneAmp 9700
`
`33x52x30
`34x60x21
`Call
`Call
`
`Cartridge
`Cartridge
`Film heater
`Peltier
`
`PE Biosystems
`800-345-5224
`www.pebio.com
`
`
`
`
`Roche Molecular Biochemicals
`30x45x40
`Electric coil/air
`
`
`
`800-428-5433
`biochem.roche.com
`
`
`
`25X30x25
`Snark Technologies
`Snark 64 and 96 models
`
`800-966-7808
`www.snarktech.com
`
`
`
`St. John Associates
`BioTherm Biooven ill
`38x43x46
`Circulating air
`
`600-837-2023
`
`www.stjohnassociates.c0m
`
`Robotic hot block
`Robotic hot block
`Robotic hot block
`
`31x49x21
`31x49><21
`31x49x21
`
`
`
`
`
`Stratagene
`800-424-5444
`www.stratagene.com
`
`
`
`
`
`RoboCycler® 40 & 96
`Infinity Temperature Cycler
`Gradient Temperature Cycler
`
`
`
`
`
`Call
`
`Call
`
`$55,000
`
`-5-105 :04
`-5-105 :04
`-9-105 10.4
`0-100 $0.4
`
`
`
`-5-100 :05
`
`4-99 20.5
`4-99 :05
`
`4-99 :0.5
`
`
`400 programs
`200 programs
`80 programs
`340 programs
`
`Call
`
`350/no Iimiflno limit
`
`
`
`99/20/20
`
`4.4
`
`1.0
`
`3.2
`
`1.0
`
`0-105 20.5
`
`$3,495
`
`Amb—100
`
`$3,990
`
`
`
`
`
`
`
`
`99/16/99
`99/16/99
`99/16/99
`
`
`
`N0 ramping
`> No ramping
`No ramping
`
`No ramping
`No ramping
`No ramping
`
`Techne
`Genius
`22x42x24
`Peltier
`Call
`99/9/99
`2.8
`
`
`800-225-9243
`Progene
`16x35x17
`Peltier
`50/9/99
`2.8
`www.techneul<.co.ul<
`TouchGene
`22x42x26
`Peltier
`3/3/—
`2.6
`
`Whatman BiomeIra®
`Personal Cycler 20 & 48
`Call
`Peltier
`No
`100/99/99
`1 800-932-7250
`T3 Thermocycler
`Peltier
`No
`Call
`www.oiometra.com
`TGradient
`Peltier
`No
`100 per directory
`TRIO Thermoolock
`Peltier
`No
`30/12/99
`UNO ll Thermocycler
`Peltier
`Call
`Peltier
`Call
`T1 Thermocycler
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`2.0
`>1.5
`4.0
`1.5
`3.3
`4.0
`
`
`
`
`
`
`
`
`
`
`
`
`
`THERMO FISHER EX. 1013
`THERMO FISHER EX. 1013
`
`

`
`22, I999
`
`THE SCIENTIST -
`
`I 9
`
`The DNA Thermal Cycler 480 is designed for small
`sample volumes, reducing reagent cost. The system has a
`48»well block that uses O.2—ml tubes and a two—tempera—
`ture protocol for lower reagent requirements.
`The GeneAmp 5700 Sequence detection system cou-
`ples the workhorse GeneAmp System 9600 with an optical
`detection system and com utcr to allow the user to
`er-
`_
`,
`P
`P
`form real—time PCR.
`
`operation. The RoboCycler Infinity has interchangeable
`40—, 96-, and 38-’l—well blocks. An in Jitli adapter is available
`for the RoboCycler 40. The RoboCycler Gradient 40 and
`96 allow testing of up to 12 annealing temperatures in one
`run. A gradient range of up to 22“C across the block with
`a difference of up to Z"C between adjacent wells can be
`programmed. All of
`the features
`included in the
`RoboCycler 40 and 96 are present as well.
`
`slides. A number of accessory features aid in
`processing and flexibility.
`
`from Idaho
`Idaho Technologies The RapidCyclcr
`Technologies can amplify a 536 bp product in under 10
`minutes with a 30—cycle reaction. This speed is possible
`with a combination of photoheating, fan cooling, and thin~
`walled capillary reaction tubes. Thin—walled microcen»
`trifuge tubes may also be used. The reduced cycling times
`also result in reduced amounts of nonspecific products.
`Cycling times can be increased by programming in hold
`steps as desired. The unit carries a one~year parts and labor
`warranty. An optional buffer system allows finished sam-
`ples to be loaded directly onto a gel.
`
`Intelligent Bio-Instruments IBI offers the TC—1600
`high—thr0ughput cycler. It is capable of running 16 blocks
`for
`a
`total of 6,144 samples
`in 96/192/384—well
`microplates. Four separate cycling profiles can be mn
`simultaneously.
`ch The latest offerin
`MJR is the PTC—
`rugged chassis are
`ngine. The sleek desi I
`hangeable sample
`fan ltalian sports car.
`. Db
`blocks and heat pump assemblies a ow the user to change
`format easily between runs while retaining NTST—traceable
`accuracy. The lab need only btiy the single control unit and
`supplement it with the desired Alpha modules, such as
`dual, independently controlled blocks, microplate format,
`or a slide adapter for up to 16 slides. Thermal control is
`achieved by either block,
`in—sample probe, or calculated
`methods. Heating rates up to 3"C/second reduce reaction
`times while :0.4°C temperature homogeneity can be
`reached within 30 seconds at 90°C. The Hot Bonnet heat—
`ed lid controls pressure and height to accommodate vari—
`ous plasticwarcsi A Power Bonnet is available for robotic
`systems. Additionally, a single cycler can be networked to
`control up to 15 separate instruments. A PC can also con—
`trol and monitor cyclers remotely and export data to video
`screens or databases in real time.
`the
`For high—throughput applications or busy labs,
`PTCv225 DNA Engine Tetrad may be the way to go. This
`allows up to eight separate blocks to run synchronous or
`independent reactions. A compact footprint is maintained
`by housing the power supply separately outside tie chassis,
`good for tight spaces or robotic applications. All ofthe fea-
`tures of the PTC—200 are present in this model as well.
`The PTC—10O cyclers incorporate a Peltier heating ele—
`ment into a more economical model that can
`e config—
`ured to accommodate plates, slides, or tubes. An optional
`heated lid is available as well as slide griddle acapters for
`amplifying up to four slides at a time. A large memory that
`holds up to 320 programs means that even crowded labs
`\ have am lc
`ro rammin s ace.
` ~ycler, the
`/IiniCycler series may be just tie ticket.
`,ncorpora ion of the Peltier—Joule heat pump increases
`cycling speed with ramping rates up to 24°C/second. The
`temperature may be programmed to allow re rigeration
`after completion of the reaction. The compact, lightweight
`unit can be easily transported to different areas of the lab.
`Power failure protection resumes cycling after temporary
`power loss up to 12 hours.
`'lwo models are available for in rim work. The PTC-
`200/Z25 models mentioned above allow use 0 the Twin
`Tower block that can hold up to 16x2 slides. The PTC-
`l00—l6MS is a dedicated configuration of the PTC—l00
`series, able to hold up to 16 double— or sitigle—thickness
`slides. The sample block is made from oxygen—free copper
`milled from a single ingot and then plated in nickel for
`durability. Memory for 200 programs, power»failure pro-
`tection, printer port, and a two-year warranty are standard.
`
`PE Biosystems The GeneAJnp® System 2400 comes stan-
`dard with a heated lid for oil—free PCR and a Z4—well sam-
`ple block. Fast
`ramping and cooling with precise
`temperature control and acr0ss—the—blocl< uniformity allow
`high-quality, reproducible reactions. The graphical inter‘
`face
`simplifies
`and speeds
`tip programming. The
`GeneAmp 9600 offers many of the same features as the
`2400 but has a 96-well sample block.
`The newer GeneAmp 9700 comes in a variety of for-
`mats. The blocks can be interchanged to accommodate 60
`0.S—ml tubes, 96 O.2—ml tubes, a 96—well plate, or dual 384-
`well plates for high—throughput needs. The system features
`a small footprint, PCMCIA card and RS485 communica-
`tions ports, Peltier heating, graphical programming inter-
`face, and an optional MAX lVIode.
`a user can choose
`V\/"ith the MAX Mode option,
`between a v
`'
`tv of so V
`'
`rt modes. In 9600 emu—
`ion mod
`e systeiiinncling profiles like those
`und in th
`ystem 9600, achieving an average heating
`d cooling rate of 35°C/second. Under Variable Ramp
`ode, the power input can be adjusted to allow a range of
`mping rates for specialized research applications. In Nlax
`ode, heating and cooling rates can be increased up to
`C/second, and sample volumes can go up to 100 pl.
`
`
`
`Techne UK Technc’s TouchGene cycler is a compact unit
`with a touchscreen display for easy interfacing. Multiple
`block options allow labs to run a variety of protocols.
`Personal memory cards let researchers store as many as
`l00 separate programs, and each program can be pass—
`word protected. Fast thermal cycling via Peltier heating
`allows a 30—cycle program to be completed in less than
`one hour to increase overall throughput. A stan
`P
`versal qtiiclcrelease heated lid adjusts to keep
`mum presstire on the sample. The aiiticondetisa
`prevents sample loss onto the side walls and ii
`board PRT sensor monitors temperature to
`reproducil‘ between samples a
`control is possible using the RS232 p
`ware, allowing the researcher to pri
`and choose, start, and stop programs.
`The Genius
`ler also has multiple interc
`block formats,
`a larger block design for
`sample capacity
`throughput. The di
`is '
`LCD. The spr
`loaded lid autoniatl adjusts the
`presstire to the
`timum for the par
`r block and
`tubes. This tinit
`features external control through the
`RS232 port.
`The low—cost Progene cycler features a very small fei-
`print, high cooling and heating speed, wide thermal range,
`and a heated lid. Two intcrchaiigeable blocks are avail—
`able—one for 25 0.2—ml tubes and 5x5 inultiwell plates and
`the other for 20 0.5—ml tubes. A special feature is the abil~
`ity to program incremental/decremental segment holds for
`Long PCR and RAPD and incremental/decremental tem—
`perature for Touchdown PCR.
`Whatman Biometra® \/Vliatman Biometra offers a wide
`range of cyclers to suit most needs. The T1 Therniocycl er
`is the flagship model, with -a number ofliigh-end features.
`Peltier heating brings fast ramping speeds of tip to 4°C/sec-
`ond heating and 3°C/second cooling. Remote control of up
`to eight units is possible with the available PC software. Lid
`pressure is variable with “smart—lid” technology. A large
`display facilitates programming with the intuitive interface.
`Microplates can be sealed during the first cycle with the
`specially developed microplates and heat—sealing film.
`The TGradicnt can create a w"
`t
`ent—up to 40°C across the plate.
`an
`tures are incorporated in this model
`heating, a gold—plated silver block,
`a
`heated lid.
`The T3 Thermo
`the features found in
`a platform with th
`blocks in a
`range 0
`Blocks are available w
`combination.
`and
`an
`18/48-well
`Microplates, strips, and tubes can all be
`accommodated. As many as 150 pro—
`grams can be stored in the memoiy. The
`heated lids can also seal microplates.
`The UNO II is a modular tinit fe-a—
`turing four different
`interchangeable
`block configurations for plates, tubes,
`and slides. The gold—plated silver
`block disperses heat uniformly while
`allowing fast heating and cooling. The
`adjustable pressure heated lid allows
`microplates
`to be
`sealed 1'77
`Jitlt.
`External PC control is possible with the
`available software.
`VVhatman's smallest model,
`the Personal Cycler has
`interchangeable 20— and 48—well blocks. The unit holds
`100 programs and features pause (for hot starts) and
`adjustable ramping functions. A four~line, 80—character
`display allows information to be displayed in ftill rather
`than in shorthand notation. An optional heated lid is avail-
`able. Documentation of the run is possible through the
`'
`'
`‘
`ts.
`here is never
`11 technology ensure
`achincs to include in
`iinual review
`
`he accompanying ta
`ill help those
`
`thermal
`
`Stratagene The RohoCyclcr® is another in
`design. The robot arm moves the samples from
`grainmablc heat block to another. Despite the seeming
`
`delay in physically moving the samples from one block to
`Gimzt Mezireiz/Jnlrie/‘
`[[777 be —t>ii'i277/Jolrle/'@
`another, cycling times are actually reduced since the block
`t/Jt>~.ta'eiztz'.r2‘.m777.
`doesn't hate to change temperature also. This also trans»
`lates to even heating of the block, allowing very tight thcr—
`mal uniformity across the block and between samples
`(:0.1°C). An optional heated lid allows oil— and wax—frce
`
`Snark Technologies For those looking
`for performance on a
`limited budget,
`Snark Technologies offers die Snark 64
`and 96 models. Both feature a heated
`lid, NIST traceable accuracy (:0.5°C),
`fast heating and cooling rates (up to
`4°C/second), wide temperature range (0
`to 105C), parallel and serial ports, a
`two—ycar warranty, easy programming,
`and a small footprint. Model 64 holds 64
`0.5 ml
`tubes while model 96 holds a
`microtiter plate or 96 0.Z—ml tubes.
`St. John Associates The BioOven III is
`one of the most unique cyclers in this
`review. Heating and cooling are done by circulating warm
`or cool air in a chamber that contains a rotating sample
`rack that can be easily switched to accommodate a variety
`of sample tubes, plates, and slides. Up to 236 0.S—inl tubes,
`10 microtiter plates, 30 slides, or hundreds of glass capil-
`lary tubes can be loaded in each run. The simplified menu-
`driven interface also includes a
`format
`feature, which
`optimizes conditions based on the type of sample bein
`run. A serial port allows users to program, format,
`access data from an external computer.
`
`Snark 64 thermal cycler
`
`
`
`For More Inf
`Circle Reader . -
`
`ation
`icc #74
`
`THERMO FISHER EX. 1013
`THERMO FISHER EX. 1013
`
`
`
`
`
`
`
`Roche Molecular Biochemicals’ LightCyc|er
`
`Roche Molecular Biochemicals Roche has licensed the
`light technology from Idaho Technology and added some
`extra features to develop the LightCycler. With a combiv
`nation of a fan and halogen bulb to cool and heat thin-
`walled glass capillary tubes, a reaction can be performed in
`less than 30 minutes. One feature that differentiates this
`product front Idaho Technologys is a built—in microvol—
`time fluorimeter that allows a user to perform real—time
`quantitative PCR. Additionally, a user can measure melt—
`ing curves, detect imitations, and simultaneously detect
`multiple
`targets. Programming and
`monitoring are done from a desktop
`PC, allowing unlimited user—defined
`complex protocols.
`
`
`
`

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