(12) United States Patent
`Cabilly et al.
`
`(10) Patent No.2
`(45) Date of Patent:
`
`US 6,331,415 B1
`Dec. 18, 2001
`
`US00633l415B1
`
`(75)
`
`(54) METHODS OF PRODUCING
`IMMUNOGLOBULINS, VECTORS AND
`TRANSFORMED HOST CELLS FOR USE
`THEREIN
`Inventors: Shmuel Cabilly, Monrovia; Herbert L.
`Heyneker, Burlingame; William E.
`H0lIIlES, Pacifica; Arthur D. Riggs, La
`Verne; Ronald B. Wetzel, San
`Francisco, all of CA (US)
`6
`V
`‘
`‘
`V
`6
`(73) Assignee: Genentigch, Inc., South San brancisco,
`CA (US
`( * ) Notice:
`Subject to any disclaimer, the term of this
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`
`(21) APPL N0‘: 07/205419
`(22)
`Filed:
`Jun. 10, 1988
`
`_
`_
`Related U.S. Application Data
`
`(63) Continuation of application No. 06/483,457, filed on Apr. 8,
`1983, now Pat. No. 4,816,567.
`
`(51)
`
`Int. Cl.7 ......................... .. C12N 15/13, C12N 15/00,
`C12N 15/63
`
`37723
`941313
`
`41767
`
`10/ 981 (SP) .
`12/ 931 (EP) ~
`Egg ~
`12/ 981 (511) .
`
`57107
`0068763
`53763
`0057107
`0073656
`075444
`73656
`75444
`A—073656
`088994
`83994
`£33253
`0125023
`194276
`196864
`234592
`255694
`324162
`550400
`08235
`62 201 581
`WO 86/01533
`
`8/ 982 GP) _
`1/ 983 (313) .
`1/ 933 (312) .
`3/ 983 (EP) .
`3/ 983 (313) .
`3/ 983 (,;P) .
`3/ 983 (3.13) .
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`7/ 989 (EP) .
`7/ 993 (EP) .
`3/ 987 (GB) .
`9/ 987 (JP)~
`3/ 986 (W0).
`OTHER PUBLICATIONS
`
`(52) U.S. Cl.
`
`..................... .. 435/69.6; 435/69.1; 435/69.7;
`435/70.1; 435/70.21; 435/711; 435/712;
`435/320; 435/252.1; 435/252.3; 435/252.33;
`435/254.11; 435/254.2; 435/254.21; 435/455;
`435/471; 435/483; 435/485
`_
`_
`(58) Field of Search ................................ .. 435/691, 69.7,
`435/71-1) 70-1) 71-2) 320) 261, 252-1) 352-3)
`81> 55> 59> 99'9> 252'33> 254'21> 483
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`Primary Examiner—Phillip Gambel
`(74) Attorney, Agent, or Firm—Burns, Doane, Swecker &
`Mathis, LLP
`
`(57)
`
`ABSTRACT
`
`.
`.
`.
`.
`The l1'lV6l']Il0l'] relates to processes for producing an immu-
`noglobulin or an immunologically functional immunoglo-
`bulin fragment containing at least the variable domains of
`the immunoglobulin heavy and light chains. The processes
`can use one or more vectors which produce both the heavy
`and light chains or fragments thereof in a single cell. The
`invention also relates to the vectors used to produce the
`immunoglobulin or fragment, and to cells transformed with
`the "°°t°rS'
`
`36 Claims, 19 Drawing Sheets
`
`Sanofi/Regeneron Ex. 1001, pg 1
`
`Merck Ex. 1001, pg 1
`
`Merck Ex. 1001, pg 1
`
`

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