Tom Huxford
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`CURRICULUM VITAE
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`Tom Huxford
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`NAME
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`DATE AND PLACE OF BIRTH
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`
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`ADDRESS AND PHONE
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`
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`July 29, 1971, Salem, Oregon, USA
`
`Department of Chemistry & Biochemistry
`San Diego State University
`5500 Campanile Drive
`San Diego, CA 92182-1030
`
`(619) 594-1606
`TEL
`(619) 594-1597
`LAB
`(619) 594-4634
`FAX
`e-mail: thuxford@mail.sdsu.edu
`web: http://www-rohan.sdsu.edu/~thuxford/
`
`
`PERSONAL DATA
`
`
`Citizenship: USA
`Family:
`Married to Glenda Auxiliadora Bojorge
`Children:
`Cyrus (son) and Aila (daughter)
`Languages:
`Fluent in English and Spanish
`
`
`EDUCATION
`
`1989-1995
`
`B.S. Honors Biochemistry with University Honors, Minor in Management
`Brigham Young University, Provo, Utah
`
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`1995-2001
`
`Ph.D. Chemistry (Biochemistry)
`University of California San Diego, La Jolla, California
`
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`PROFESSIONAL TRAINING AND POSITIONS
`
`1993-1995
`
`Research Associate, Analytical Chemistry Laboratory of Professor John D. Lamb
`Brigham Young University
`Honors Thesis: “Quantitative Analysis of Cyanide and Sulfide Ions from Basic Waste Solutions
`by Capillary Zone Electrophoresis”
`
`
`1995-2001 Graduate Student, Structural Biology Laboratory of Professor Gourisankar Ghosh
`University of California San Diego
`Dissertation: “Inactivation of Transcription Factor NF-κB through Its Association with the IκBα
`Inhibitor Protein”
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`Tom Huxford
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`2001-2005
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`Postdoctoral Fellow, University of California Universitywide AIDS Research Program,
`Structural and biophysical characterization of proteins and complexes involved in the NF-κB
`signal transduction pathway. Structures of Aplysia acetylcholine binding protein:ligand
`complexes.
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`2005-2010 Assistant Professor, Department of Chemistry & Biochemistry, San Diego State University
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`2005-2010 Assistant Professor, Molecular Biology Institute, San Diego State University
`
`2010-2014 Associate Professor, Department of Chemistry & Biochemistry, San Diego State University
`
`2010-2014 Associate Professor, Molecular Biology Institute, San Diego State University
`
`PRESENT POSITIONS
`
`2014-
`2014-
`2006-
`
`HONORS AND AWARDS
`
`1989
`1998
`2001
`2002
`
`Professor, Department of Chemistry & Biochemistry, San Diego State University
`Professor, Molecular Biology Institute, San Diego State University
`Director, San Diego State University Macromolecular X-ray Crystallography Facility
`
`Ezra Taft Benson Scholarship (University Presidential), Brigham Young University
`Predoctoral Fellowship, University of California Universitywide AIDS Research Program
`Postdoctoral Fellowship, University of California Universitywide AIDS Research Program
`Martin D. Kamen Prize, Awarded annually to the outstanding dissertation defended in the field
`of biochemistry at the University of California San Diego
`Research Scholar, American Cancer Society
`Outstanding Faculty Award, Nu Alpha Kappa Latino-Based Fraternity, San Diego State
`University
`Outstanding Faculty Award, Department of Chemistry & Biochemistry, San Diego State
`University
`Mortar Board Award for Leadership, Scholarship, Service, nominated by SDSU students
`Kimberly Ariza and Kyle Grant
`Favorite Faculty Member recognition by SDSU Residential Students
`Outstanding Faculty Award, nominated by College of Sciences Outstanding Student Linda
`Honaker
`Favorite Faculty Member recognition by SDSU Residential Students
`Favorite Faculty Member recognition by SDSU Residential Students
`
`2009
`2009
`
`2009
`
`2011
`
`2014
`2014
`
`2015
`2016
`
`PROFESSIONAL SERVICE
`
`2005-2013
`2006
`2006
`2007-2008
`
`Reviewer, Argonne National Laboratory Advanced Photon Source
`Textbook Reviewer, Tymoczko, Berg, and Stryer, Biochemistry: A Short Course, W.H. Freeman
`Reviewer, Utah State University New Faculty Research Grant Program
`Reviewer, California State University Program in Education and Research in Biotechnology
`(CSUPERB) Grant Programs
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`2007
`2008
`2009
`2009
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`2010
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`2010
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`2010
`
`2010
`2011
`2011
`2011
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`2011
`
`2011
`2011
`2011
`2012
`
`2012
`
`2012
`2012
`2012
`
`2012
`2013
`2013
`
`2013
`
`2013
`2013
`2013
`
`2014
`2014
`
`2014
`2014
`2014
`2015
`
`Reviewer, Singularity, California Educational Alliance science journal for High School Students
`Textbook Reviewer, Smith, General, Organic, & Biological Chemistry, McGraw-Hill
`Reviewer, European Journal of Medicinal Chemistry
`Reviewer, Academic Research Infrastructure Program: Recovery and Reinvestment (ARI-R2),
`National Science Foundation, Arlington, Virginia, October 7-8, 2009
`Reviewer, California State University Program in Education and Research in Biotechnology
`(CSUPERB) Grant Programs
`Reviewer, American Heart Association, Western States Affiliate, Peer Review Committee 3A,
`Burlingame, CA, April 22, 2010
`Reviewer, National Institutes of Health/National Institute of Diabetes and Digestive and Kidney
`Diseases, Diabetes, Endocrinology, and Metabolic Diseases B Subcommittee, San Diego, CA,
`June 16-18, 2010
`Reviewer, Journal of Signal Transduction
`Textbook Reviewer, Lodish, et al., Molecular Cell Biology, 7th Ed., W.H. Freeman
`Textbook Reviewer, Voet, Voet & Pratt, Fundamentals of Biochemistry, 4th Ed., Wiley
`Reviewer, Chemistry of Life Processes Program, National Science Foundation, Arlington,
`Virginia
`Textbook Writer/Editor, Lodish, et al., Molecular Cell Biology, 7th Ed., W.H. Freeman, End of
`chapter problems, Chapters 2 and 3
`Reviewer, Journal of Signal Transduction
`Reviewer, Biophyscial Journal
`Reviewer, Journal of Applied Crystallography
`Reviewer, California State University Program in Education and Research in Biotechnology
`(CSUPERB) Grant Programs, Pomona, CA, April 14, 2012
`Reviewer, American Heart Association, Proteins and Crystallography 4 Committee, April 18,
`2012
`Reviewer, PLoS Computational Biology
`Reviewer, Letters in Drug Design & Discovery
`Reviewer, American Heart Association, Proteins and Crystallography 2 Committee, October 15,
`2012
`Reviewer (ad hoc), Chemistry of Life Processes Program, National Science Foundation
`Reviewer, Chemistry of Life Processes Program, National Science Foundation, Virtual Panel
`Reviewer (ad hoc), Blue-Sky (Blanc) Programme, French National Research Agency, March 18,
`2013
`Reviewer, American Heart Association, Proteins and Crystallography 5 Committee, April 30,
`2013
`Reviewer, Journal of Inorganic Biochemistry
`Reviewer (ad hoc), Multiple Sclerosis Research Australia, August 15, 2013
`Reviewer, American Heart Association, Proteins and Crystallography 3 Committee, April 30,
`2013
`Reviewer, Stanford Synchrotron Radiation Light Source
`Reviewer, American Heart Association, Proteins and Crystallography 5 Committee, April 17,
`2014
`Reviewer, Cell Biochemistry and Function
`Reviewer, Chemistry of Life Processes Program, National Science Foundation, Virtual Panel
`Reviewer (ad hoc), Biotechnology and Biological Sciences Research Council, December, 2014
`Reviewer (ad hoc), Biotechnology and Biological Sciences Research Council, March, 2015
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`Reviewer, American Heart Association, Proteins and Crystallography 3 Committee, St.
`Petersburg, FL, April 16, 2015
`Reviewer (ad hoc), Biotechnology and Biological Sciences Research Council, August, 2015
`Reviewer, Scientific Reports
`Reviewer (ad hoc), Biotechnology and Biological Sciences Research Council, November, 2015
`Reviewer (ad hoc), Chemistry of Life Processes Program, National Science Foundation
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`Tom Huxford
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`2015
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`2010-2014
`2011-2013
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`2015
`2015
`2015
`2016
`
`UNIVERSITY SERVICE
`
`Coordinator, Pfizer Undergraduate Research Fellowship Program
`2005
`2006-2007 Member, Faculty Search Committee, Analytical Chemistry, Department of Chemistry &
`Biochemistry
`2006-2009 Member, Radiation Safety Committee, San Diego State University
`2006-2009 Member, Scholarship Committee, Department of Chemistry & Biochemistry
`2006-
`Member, Graduate Committee, Department of Chemistry & Biochemistry
`2007-2008 Member, Faculty Search Committee, Structural Biology, Department of Biology
`2008-
`Director, San Diego State University Structural Biology Program
`2008-
`Member, Student Research Committee, San Diego State University
`2010-2014 Member, Preprofessional Health Student Evaluation Committee, San Diego State University
`2010-2011 Member, Faculty Search Committee, Organic Chemistry, Department of Chemistry &
`Biochemistry
`Chair, Scholarship Committee, Department of Chemistry & Biochemistry
`Chair, Reappointment, Tenure, and Promotion Committee, Department of Chemistry &
`Biochemistry
`Member, Advisory Committee, SDSU/CIRM Stem Cell Internship Program
`2012-
`2013-2014 Member, Faculty Search Committee, Analytical Chemistry, Department of Chemistry &
`Biochemistry
`2013-2014 Member, Faculty Search Committee, Structural Biology, Department of Biology (Viromics
`Institute cluster hire)
`Member, Advisory Board, Barry M. Goldwater Scholarship
`2014-
`2014-2015 Member, Reappointment, Tenure, and Promotion Committee, College of Sciences
`2014-2015
`Chair, Faculty Search Committee, Physical Biochemistry, Department of Chemistry &
`Biochemistry
`2014-2015 Member, Faculty Search Committee, Immunology, Department of Biology
`2014-2015 Member, Joint Doctoral Program Executive Committee, Department of Biology
`2014-2015 Member, Faculty Search Committee, Computational Chemistry, Department of Chemistry &
`Biochemistry (Viromics Institute cluster hire)
`Member, Special Review Panel, College of Sciences Policy and Planning Committee
`2015
`Chair, Reappointment, Tenure, and Promotion Committee, College of Sciences
`2015-2016
`2015-2016 Member, Faculty Search Committee, Immunology, Department of Biology
`
`COURSES TAUGHT
`
`Chem 560A – General Biochemistry
`Fall 2005
`Spring 2006 Chem 560A – General Biochemistry
`Fall 2006
`Chem 365 – Biochemistry, Cell & Molecular Biology I
`Spring 2007 Chem 100 – Introduction to General Chemistry
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`Chem 100 – Introduction to General Chemistry
`Fall 2007
`Spring 2008 Chem 365 – Biochemistry, Cell & Molecular Biology I
`
`MBio 610 – Advanced Topics in Molecular Biology (Guest lecturer, Structural Biology)
`Summer 2008 Chem 365 – Biochemistry, Cell & Molecular Biology I
`Fall 2008
`Chem 365 – Biochemistry, Cell & Molecular Biology I
`
`MBio 610 – Advanced Topics in Molecular Biology (Guest lecturer, Structural Biology)
`Spring 2009 Chem 100 – Introduction to General Chemistry
`Fall 2009
`Chem 100 – Introduction to General Chemistry
`Spring 2010 Chem 560 – General Biochemistry
`
`
`Chem 763 – Cellular Regulation (Team taught with Dr. William Stumph)
`
`
`Chem 790 – Biochemistry Graduate Seminar
`
`
`MBio 610 – Advanced Topics in Molecular Biology (Guest lecturer, Structural Biology)
`Summer 2010 Chem 365 – Biochemistry, Cell & Molecular Biology I
`Fall 2010
`Chem 365 – Biochemistry, Cell & Molecular Biology I
`Spring 2011 Chem 560 – General Biochemistry
`
`
`Chem 790 – Biochemistry Graduate Seminar
`
`
`Chem 791 – Biochemistry Research Seminar
`
`
`MBio 610 – Advanced Topics in Molecular Biology (Guest lecturer, Structural Biology)
`Fall 2011
`Chem 365 – Biochemistry, Cell & Molecular Biology I
`Spring 2012 Chem 562 – Intermediary Metabolism
`
`
`Chem 790 – Biochemistry Graduate Seminar
`
`
`Chem 791 – Biochemistry Research Seminar
`Fall 2012
`Chem 100 – Introduction to General Chemistry
`Spring 2013 Chem 562 – Intermediary Metabolism
`
`
`Chem 790 – Biochemistry Graduate Seminar
`
`
`Chem 791 – Biochemistry Research Seminar
`Summer 2013 Chem 365 – Biochemistry, Cell & Molecular Biology I
`Fall 2013
`Chem 100 – Introduction to General Chemistry
`Spring 2013 Chem 763 – Cellular Regulation: Biophysical Biochemistry
`
`
`Chem 790 – Biochemistry Graduate Seminar
`
`
`Chem 791 – Biochemistry Research Seminar
`Fall 2014
`Chem 100 – Introduction to General Chemistry
`
`
`Chem 560 – General Biochemistry
`Spring 2015 Chem 562 – Intermediary Metabolism
`
`
`Chem 790 – Biochemistry Graduate Seminar
`Fall 2015
`Chem 100 – Introduction to General Chemistry
`
`
`Chem 560 – General Biochemistry
`Spring 2016 Chem 562 – Intermediary Metabolism
`
`
`Chem 790 – Biochemistry Graduate Seminar
`
`SEMINAR SPEAKERS HOSTED
`
`1) Dr. Bradley J. Nolen, Department of Molecular Cellular & Developmental Biology, Yale University.
`“Biophysical studies of Arp2/3 complexes and dynamics of actin filaments.” SDSU Molecular Biology
`Institute Seminar. December 7, 2006.
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`2) Dr. Case McNamara, Structural Biology/Drug Discovery, The Genomics Institute of the Novartis Research
`Foundation. “The twisted molecular design of the streptococcal M1 protein.” SDSU Structural Biology
`Program Seminar. December 3, 2008.
`3) Dr. Jeff Lee, Department of Immunology, The Scripps Research Institute. “Techniques, tools and tactics
`used in the structure determination of the Ebola virus glycoprotein.” SDSU Structural Biology Program
`Seminar. December 10, 2008.
`4) Dr. Melanie Cocco, Department of Molecular Biology and Biochemistry, University of California Irvine.
`“Proteins on the edge (of the lipid bilayer).” SDSU Department of Chemistry & Biochemistry Seminar.
`October 16, 2009.
`5) Dr. Ulrich Müller, Department of Chemistry & Biochemistry, University of California San Diego.
`“Catalytic RNAs for mRNA repair." SDSU Department of Chemistry & Biochemistry Seminar. April 9,
`2010.
`6) Dr. Hector Viadiu, Department of Chemistry & Biochemistry, University of California San Diego.
`“Structural studies of p53 regulation.” SDSU Department of Chemistry & Biochemistry Seminar. April 30,
`2010.
`7) Dr. Teru Nakagawa, Department of Chemistry & Biochemistry, University of California San Diego.
`“Molecular assembly of AMPA receptors.” SDSU Molecular Biology Institute Seminar. May 6, 2010.
`8) Dr. Nathan Gianneschi, Department of Chemistry & Biochemistry, University of California San Diego.
`“DNA, peptides and enzymes as building blocks for polymeric nanoscale materials.” SDSU Department of
`Chemistry & Biochemistry Seminar. November 12, 2010.
`9) Dr. Feng Guo, Department of Biological Chemistry, University of California Los Angeles School of
`Medicine. “Potential functions of heme and iron in microRNA biogenesis.” SDSU Department of
`Chemistry & Biochemistry Seminar. April 22, 2011.
`10) Dr. Maitrayee Das Gupta, Department of Biochemistry, Calcutta University. “Structure/Function
`similarities between a plant receptor-like kinase and the human Interleukin-1 Receptor Associated Kinase-
`4.” SDSU Department of Chemistry & Biochemistry Seminar. June 28, 2011.
`11) Dr. Bradley J. Nolen, Department of Chemistry and Institute of Molecular Biology, University of Oregon.
`“How small molecule inhibitors and regulatory proteins control actin filament nucleation mediated by
`Arp2/3 complex.” SDSU Department of Chemistry & Biochemistry Seminar. November 4, 2011.
`12) Dr. Scott B. Hansen, Laboratory of Molecular Neurobiology & Biophysics, Rockefeller University. “A
`crystal clear picture for PIP2 activation of the inward rectifying postassium channel Kir.” SDSU
`Department of Chemistry & Biochemistry Seminar. December 2, 2011.
`13) Dr. Randall T. Peterson, Charles and Ann Sanders Massachusetts General Hospital Reseach Scholar,
`Harvard Medical School. “Fish, heatmaps, and the discovery of neuroactive drugs.” SDSU Biological
`Sciences Seminar. February 6, 2012.
`14) Dr. Joel Griffitts, Department of Microbiology & Molecular Biology, Brigham Young University.
`“Understanding instability in the rhizobium-legume symbiosis.” SDSU Molecular Biology Institute
`Seminar. April 26, 2012.
`15) Dr. Malini Sen, Cancer Biology & Inflammatory Disorder Division, Indian Institute of Chemical Biology.
`“Role of Wnt signaling in monocyte/macrophage survival and phagocytosis.” SDSU Molecular Biology
`Insitute Seminar. September 20, 2012.
`16) Dr. Edith (Phoebe) Glazer, Department of Chemistry, University of Kentucky. “Exploiting coordination
`chemistry for the development of novel anticancer agents.” SDSU Department of Chemistry &
`Biochemistry Seminar. October 12, 2012
`17) Dr. Katherine Kantardjieff, College of Physical Science and Mathematics, California State University—San
`Marcos. “The (mis)conduct of science in the information age.” SDSU Department of Chemistry &
`Biochemistry Seminar. October 19, 2012
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`18) Dr. Simpson Joseph, Department of Chemistry & Biochemistry, University of California San Diego.
`“Mechanism of translational control by the Fragile X mental retardation protein.” SDSU Department of
`Chemistry & Biochemistry Seminar. October 26, 2012
`19) Dr. Phil Dawson, Department of Chemistry, The Scripps Research Institute. “Chemoselective approaches
`for the synthesis of proteins and assembly of nanoparticles.” SDSU Department of Chemistry &
`Biochemistry Seminar. February 8, 2013
`20) Dr. Ulrich Müller, Department of Chemistry & Biochemistry, University of California San Diego. “A new
`ribozyme for the RNA world.” SDSU Department of Chemistry & Biochemistry Seminar. May 3, 2013.
`21) Dr. Lei Wang, The Jack H. Skirball Center for Chemical Biology & Proteomics, The Salk Institute for
`Biological Sciences. “Genetically encoding unnatural amino acids to study signaling proteins.” SDSU
`Department of Chemistry & Biochemistry Seminar. May 10, 2013.
`22) Dr. Steven Johnson, Department of Microbiology and Molecular Biology, Brigham Young University.
`“Chromatin patterns, meta-shapes and transgene expression.” SDSU Biology Department Seminar. June
`24, 2013.
`23) Dr. Tapan Biswas, Department of Chemistry & Biochemistry, University of California San Diego.
`“Bacteriophage Lambda DNA integrase structure and mechanism.” SDSU Department of Chemistry &
`Biochemistry Seminar. October 11, 2013
`24) Dr. Erica Ollman Saphire, Department of Immunology & Microbial Science, The Scripps Research
`Institute. “Ebola virus matrix: Structural plasticity begets multiple functions in the virus life cycle.” SDSU
`Molecular Biology Institute Seminar. October 24, 2013.
`25) Dr. Pieter Dorrestein, Institute for Metabolomics Medicine, Skaggs School of Pharmacy & Pharmaceutical
`Sciences, Departments of Pharmacology, Chemistry and Biochemistry, University of California San Diego.
`“A ‘GoogleMAP’-type molecular view of microbes—from culture to people.” SDSU Department of
`Chemistry & Biochemistry Seminar. October 25, 2013
`26) Dr. Bog Stec, Sanford-Burnham Medical Research Institute. “Trapping and visualization of catalytic
`intermediates.” SDSU Department of Chemistry & Biochemistry Seminar. March 14, 2014
`27) Dr. Eileen J. Kennedy, Department of Pharmaceutical & Biomedical Sciences, College of Pharmacy,
`University of Georgia. “Design of chemically modified peptides to study regulation of protein kinases.”
`SDSU Department of Chemistry & Biochemistry Seminar. December 12, 2014
`28) Dr. Neal Devaraj, Department of Chemistry & Biochemistry, University of California San Diego.
`“Advancing bioorthogonal chemical reactions for applications in imaging and bottom-up synthetic biology.”
`SDSU Department of Chemistry & Biochemistry Seminar. March 13, 2015
`29) Dr. Jacky Chi Ki Ngo, School of Life Sciences, The Chinese University of Hong Kong. “Splicing kinase
`SRPK2 possesses distinct mechanisms for the phosphorylation of SR proteins.” SDSU Molecular Biology
`Insitute Seminar. June 23, 2015
`30) Dr. Roy Wollman, Department of Chemistry & Biochemistry, University of California San Diego.
`“Reliable signal transduction.” SDSU Department of Chemistry & Biochemistry Seminar. September 4,
`2015
`31) Dr. Nathan E. Lewis, Department of Pediatrics, University of California San Diego School of Medicine.
`“Elucidating post-translational modification function in the whole cell context: How the PTM tail wags the
`dog.” SDSU Department of Chemistry & Biochemistry Seminar. October 9, 2015
`32) Dr. Jacob Durrant, Department of Chemistry & Biochemistry, University of California San Diego.
`“Computer-aided drug design: From basic science to clinical applications.” SDSU Department of
`Chemistry & Biochemistry Seminar. January 22, 2016
`33) Dr. James McKerrow, Skaggs School of Pharmacy, University of California San Diego. “Developing drugs
`for neglected tropical diseases.” SDSU Department of Chemistry & Biochemistry Seminar. February 5,
`2016
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`34) Dr. Corey Dambacher, Department of Integrative Structural and Computational Biology, The Scripps
`Research Institute. “Electron microscopy analysis of the proteasome deubiquitinases from low to atomic
`resolution.” SDSU Department of Chemistry & Biochemistry Seminar. February 19, 2016
`
`
`EXTERNALLY FUNDED RESEARCH
`
`2005-2007
`
`
`2007
`
`
`
`
`2008
`
`
`2008-2011
`
`
`2009-2012
`
`
`2009-2013
`
`
`2009
`
`
`2009-2010
`
`
`2011-2014
`
`
`2013-2018
`
`Principal investigator, “Murr1 inhibition of NF-κB-dependent HIV transcription”
`Universitywide AIDS Research Program Idea Grant ID05-SDSUF-014, $125,000
`
`Principal investigator, “Biochemical characterization of human and fly IκB kinases”
`CSU Program in Education and Research in Biotechnology (CSUPERB)
`Faculty-Student Collaborative Research Seed Grant, $13,500
`
`Principal investigator, “Confirming target gene silencing by cytotoxic antisense
`oligonucleotides”
`Sky Genetics, Inc., $42,577
`
`Collaborator, “Structure of myosin chaperone UNC-45 and its role in skeletal muscle
`disease”—Dr. Sanford I. Bernstein, PI
`NIH/National Institute of Arthritis and Musculoskeletal and Skin Diseases
`R01AR055958, $540,000
`
`Principal investigator, “Structural biology of the IκB Kinase beta subunit”
`American Cancer Society Research Scholar Grant
`RSG-08-287-01-GMC, $600,000
`
`Collaborator, “Genetics and molecular biology of myosin”—Dr. Sanford I. Bernstein, PI
`NIH/National Institute of General Medical Sciences
`R01GM32443, $1,125,000
`
`Collaborator, “Structure based design of humanized monoclonal antibodies that recognize
`bioactive lipids”—Dr. Jon Wojciak, PI
`NIH/National Institute of General Medical Sciences
`R43GM088956-01, $113,129
`
`Collaborator, “ARRA: Pathways of lambda site-specific recombination”—Dr. Anca Segal, PI
`NIH/National Institute of General Medical Sciences
`R01GM052847-11S1, $195,378
`
`Principal investigator, “Chemistry and evolutionary conservation of a naturally occurring
`metalloantibody”
`NSF/Chemistry: Chemistry of Life Processes
`CHE-1112547, $400,000
`
`Collaborator, “Mechanism of myosin chaperone UNC-45: Structural, functional &
`genetic approaches”—Dr. Sanford I. Bernstein, PI
`NIH/National Institute of Arthritis and Musculoskeletal and Skin Diseases
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`2014-2015
`
`R01AR055958-04, $1,588,440
`
`Collaborator, “Genetics and molecular biology of striated muscle myosin”—Dr. Sanford
`I. Bernstein, PI
`NIH/National Institute of General Medical Sciences
`R01GM032443-28, $1,850,659
`
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`Principal investigator, “Measuring equilibrium binding affinity of biological
`macromolecules in soution by thermophoresis”
`DoD/Research and Education Program for Historically Black Colleges and Universities
`and Minority-Serving Institutions (HBCU/MI) Equipment/Instrumentation
`W911NF-14-1-0082, $143,238
`
`
`INTERNALLY FUNDED RESEARCH
`
`2005
`
`Principal investigator, “Three dimensional structure of two curariform neurotoxins bound to the
`Aplysia acetylcholine binding protein”
`SDSU University Grants Program FGIA and FDP programs, $9,000
`
`
`2010
`
`Principal investigator, “Identification of a novel functional domain of human STS-1”
`SDSU University Grants Program, $10,000
`
`
`GRADUATE STUDENTS SUPERVISED
`
`1) Dan V. Trinh, M.S. Chemistry & Biochemistry, Fall 2005-Fall 2007 “Biochemical
`characterization of IκBζ interactions with NF-κB/DNA complexes”
`2) Jacob D. Shaul, M.S. Cellular & Molecular Biology, Spring 2006-Spring 2008 “IKKβ
`phosphorylates IκBα with CK2-like specificity upon removal of C-terminal elements”
`3) Ghazal Farhang, M.S. Chemistry & Biochemistry, Spring 2007-Summer 2008 “Assaying
`transcriptional activation by NF-κB p50 Homodimer/IκBζ complexes on κB DNA promoters”
`4) Michaela Norrbom, M.S. Chemistry & Biochemistry, Spring 2007-Fall 2008 “Expression,
`purification, and in vitro characterization of the Drosophila melanogaster IκB Kinase β subunit”
`5) Jon Fleming, M.S. Cellular & Molecular Biology, Fall 2009-Summer 2010 “Crystallization and
`x-ray structure determination of the antigen binding portion of a humanized monoclonal anti-
`lysophosphatidic acid antibody”
`6) Jesal Patel, M.S. Chemistry & Biochemistry, Fall 2009-Fall 2010 “Biochemical characterization
`of a phosphate-binding domain in STS-1”
`7) Mark Villaluz, M.S. Chemistry & Biochemistry, Fall 2007-Fall 2010 “Purification and kinetic
`characterization of soluble Sphingomyelinases C and D”
`8) Danielle DiTirro, M.S. Cellular & Molecular Biology, Fall 2009-Spring 2011 “Large-scale
`expression and characterization of a human multi-subuit IKK complex”
`9) Frank Erasmus, M.S. Cellular & Molecular Biology, Fall 2011-Fall 2012 “Characterization of
`metal binding by the anti-sphingosine-1-phosphate metalloantibody LT1002”
`10) Deepthi Anipindi, M.S. Chemistry & Biochemistry, Fall 2010-Fall 2012 “X-ray crystal
`structures of two thermostable metal binding variants of Streptococcal Protein Gβ1 domain”
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`11) Ashlee King, M.S. Chemistry & Biochemistry, Fall 2011-Spring 2013 “Crystallization of
`nucleic acid complexes: NF-κB p50 homodimer bound to G-IRE sequences and Holliday
`junctions bound to inhibitors of recombination”
`12) Norman Zhu, Ph.D. Chemistry & Biochemistry, Fall 2005-Spring 2013 “Structure and
`biochemistry of IκBζ: A nuclear IκB protein”
`13) Arthur Hauenstein, Ph.D. Chemistry & Biochemistry, Spring 2006-Spring 2013 “The role of
`oligomerization in human IKK2 activation as revealed by structural and biophysical studies”
`14) James Caldwell, Ph.D. Chemistry & Biochemistry, Fall 2005-Fall 2013 “First x-ray crystal
`structure of an insect cell myosin”
`15) Scott Lashbrook, M.S. Chemistry & Biochemistry, Fall 2011-Fall2014 “In vitro analysis of
`tyrosine auto-phosphorylation by the IKK2 enzyme”
`16) Christy Milani, M.S. Cellular & Molecular Biology, Fall 2013-Spring 2015 “Ideal DNA binding
`site spacing by NF-κB p50 homodimer”
`17) W. Eric Rogers, Ph.D., Fall 2011
`18) Ellie Nguyen, M.S., Fall 2013
`19) Elinaz Farokhi, Ph.D., Fall 2014
`20) Samantha Cohen, Ph.D., Fall 2014
`21) Tom McDowell, M.S., Fall 2015
`
`UNDERGRADUTE STUDENT SPECIAL STUDY RESEARCH SUPERVISED
`
`1) Heath Metzler (Spring 2006) “Murr1”
`2) Yekaterina Y. Sharikova (Spring 2006) “Bioinformatic approach for studying IκB Kinases”
`3) Erin Takahashi (Spring 2006) “Differences in protein-protein interactions between IκBα and
`IκBζ”
`4) Sean Cahanding (Summer 2006) “Crystallization of a complex between NF-κB p65 homodimer
`and IκBα”
`5) Jason Abbott (Fall 2006) “IκBNS”
`6) Natalie Jamgochian (Fall 2006) “IκBζ shows stronger binding affinity for p50 NF-κB factor
`than p65”
`7) Danielle Lucas (Fall 2006) “Patenting research inventions in a university setting”
`8) Candice Alcantara (Spring 2007). “A C-terminal fragment of the Drosophila NF-κB protein
`Relish”
`9) Nicholas Brunn (Spring 2007) “Analysis of nuclear IκB binding to NF-κB dimers, with
`emphasis on Bcl-3 and IκBζ”
`10) Nicole Glenn (Spring 2007) “The cloning of N-terminal COMMD1 (1-108) and the expression
`and purification of Cul1-Rbx1 in BL21(DE3) E. coli”
`11) Roberto Martinez (Spring 2007) “Cloning of IκBNS”
`12) NikiSadat Mehdizadegan (Summer 2007) “The expression and purification of GST-IκBε”
`13) Brian Kim (Fall 2007) “The cloning and purification of NF-κB p50 and p65 protein constructs
`for application in future investigation”
`14) Javier Bravo (Fall 2007) “COMMD1 (Murr1) binds to the Cul1 component of SCF-Type
`ubiquitin-protein ligases”
`15) Daniel Unzicker (Spring 2008) “Cloning, expression, and purification of SMase C and D in
`BL21 (DE3) E. coli”
`16) May Oo (Spring 2008) “Expression and purification of GST-IκBNS for future investigations”
`
`Page 10 of 27
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`Tom Huxford
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`17) Ngan Le (Spring 2008) “Obstacles encountered during the purification of IκBε and proposed
`solutions”
`18) Marvin Minas (Spring 2008) “Transformation and expression of COMMD6 and p50 in BL21
`(DE3) E. coli”
`19) Jonathan Fleming (Fall 2008) “Identification of a minimal complex for IκBζ-assisted
`transcriptional activation” and “Purification of apo LT1009 Fab fragment”
`20) Vincent Siu (Fall 2008) “Interaction between HIV Vpr and human importin-α3”
`21) Skylar Clark (Fall 2008) “Expression and purification of corticotropin releasing hormone
`binding protein (CRH-BP) from SF9 cells”
`22) Patricia Crivello (Spring 2009) “The VP1 capsid protein from JC polyomavirus”
`23) Tony Dao (Fall 2008) “Protein purification of Loxosceles arizonica SMAse D”
`24) Jawan Iskander (Spring 2009) “Cloning the BK viral capsid in XL1-Blue E. coli cells”
`25) Kevin Carman (Spring 2009) “Expression and purification of Taq DNA polymerase in BL21
`(DE3) pLysS E. coli”
`26) Silvestre Rosas (Fall 2009) “Investigation into the binding of IκBζ to NF-κB”
`27) Zachary Gorden (Fall 2009) “Creating a BK-VP1 expression plasmid”
`28) Matthuse Ramsis (Spring 2009) “Purification of Sphingomyelinase D dimer”
`29) Valerie Engelke (Spring 2009) “Purification of the TPR domain of DmUNC-45 for x-ray
`crystallography”
`30) Michael Niemeyer (Spring 2009) “Cloning of Drosophila Hsp83 cDNA from S2 cells”
`31) Jacqueline Fritz (Fall 2010) “Recombinant expression and purification of Cactus fragment 1-
`124 and Full length: An IκB homolog in Drosophila melanogaster”
`32) Allen Washington, Jr. (Fall 2010) “Purification of active suppressor of T-cell receptor signaling
`(STS-1) phosphotyrosine binding motif”
`33) Ali Hejazi (Spring 2011) “Investigating the interaction of Drosophila UNC-45 and Hsp83”
`34) Maria Gutierrez (Spring 2011) “Phosphate dependency in interfacial metal bridging of an
`antibody and its antigen”
`35) Cayla Martin (Spring 2011) “Cloning of Green Fluorescent Protein-Tagged Drosophila
`melanogaster UNC-45”
`36) Cathrine Aivati (Fall 2011) “Structure-based design and cloning of a stable IKK2 construct”
`37) Ahmed Farah (Fall 2011) “Crystallization and x-ray diffraction of a mutant oligomeric protein
`G variant”
`38) Thaer Othman (Fall 2011) “Purification and crystallization of FbpA”
`39) Dina Yacoub (Fall 2011) “Measuring metal binding affinity of the LT1002 antibody by flame
`atomic absorption spectroscopy”
`40) Karen Nguyen (Spring 2012) “Expression and purification of NEMO”
`41) Sarah Albayati (Spring 2012) “Expression and purification of human lysine-specific
`demethylase 1 (LSD1) in E. coli”
`42) Rodaw Othman (Spring 2012) “Purification and crystallization of an hLSD1:CoREST1:
`Compound complex”
`43) Mahosoth Thosy (Spring 2012) “An expression and purification protocol for recombinant
`human STS-1 Shc-binding domain”
`44) Merta Cushing (Fall 2012) “The expression and purification of transcription factor Ets-1”
`45) Christy Milani (Fall 2012) “Crystallization of a p50 homodimer:G-rich IRE DNA complex”
`46) Megan Almazan (Fall 2013) “Hsp90 binding and interaction with UNC-45”
`47) Aaron Ward (Fall 2013) “Distinct metalloantibodies use a similar binding motif for bridging
`metal cations”
`
`Page 11 of 27
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`Exhibit 1003
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`Tom Huxford
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`Page 12
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`48) Abdigani Ali (Fall 2013) “Crystallization and x-ray diffraction of proteins”
`49) Daniela Gouran (Fall 2013) “Preparation of a GST-tagged UNC-45 expression plasmid”
`50) Hannah Lollar (Fall 2013) “Hsp90 interaction with UNC-45”
`51) Jeremy Borbon (Spring 2014) “Expression and purification of NF-κB p50 homodimers”
`52) Kayla Schenk (Spring 2014) “An assay for detecting phosphoesterase activity in an
`evolutionarily conserved metalloantibody”
`53) Joshua Beverly (Spring 2014) “Mutagenesis of IκBζ and binding to p50”
`54) Linda Honaker (Spring 2014) “Measuring NF-κB binding to κB DNA by MicroScale
`Thermophoresis”
`55) Arianne Salunga (Fall 2014) “Recombinant expression and purification of Drosophila LaminC
`protein”
`56) Lisa Acuna (Fall 2014) “Binding of κB sequence DNA by the NF-κB p50 homodimer”
`57) Thomas McDowell (Fall 2014) “Structural and functional elucidation of the Drosophila
`melanogaster IκB Kinase”
`58) Jeffrey Noblet (Fall 2014) “MicroScale Thermo